Alerta

Application of salmonella bacteriophage vBSalDSP13 and rapid detection method of salmonella bacteriophage vBSalDSP13

Resumen de: CN122214285A

The invention discloses application of a salmonella bacteriophage vBSalDSP13 and a rapid detection method of the salmonella bacteriophage vBSalDSP13. The preservation number of the bacteriophage vBSalDSP13 is GDMCC No: 67876-B1, and the preservation number of the bacteriophage vBSalDSP13 The virulent bacteriophage vBSalDSP13 provided by the invention is wide in host spectrum, can split up to 25 salmonella serotypes, can split two strains of escherichia coli at the same time, and has a characteristic molecular target. The bacteriophage SP13 has good thermal stability, pH stability and chloroform tolerance, is not easy to inactivate in the application process, and has a good inhibition effect on target bacteria in milk and chicken. In conclusion, the bacteriophage SP13 can be used as a single inhibitor or combined with other pathogenic bacteria bacteriophages to form an inhibitor for preventing and controlling pathogenic bacteria such as salmonella and the like.

一种Ulp1酶双抗夹心ELISA试剂盒及应用

Resumen de: CN120992924A

The invention belongs to the technical field of Ultrap1 enzyme quantitative detection, and particularly relates to an Ultrap1 enzyme double-antibody sandwich ELISA (enzyme-linked immuno sorbent assay) kit and application. Comprising an elisa plate coated with an anti-Ultrap1 enzyme mouse monoclonal antibody, an anti-Ultrap1 enzyme rabbit polyclonal antibody, an HRP-labeled goat anti-rabbit antibody, a diluent, a cleaning solution, a confining solution, a developing solution and a stop solution. According to the present invention, the complex of the Ulp1 enzyme and the SUMO tag protein is adopted as the immunogen to simultaneously immunize mice and New Zealand rabbits so as to prepare different species of the good affinity Ulp1 enzyme antibodies, and then the optimal reaction condition is further screened to develop the stable double-antibody sandwich ELISA detection kit with characteristics of high specificity and strong sensitivity, the defects in the prior art can be effectively overcome.

一种htrA基因敲除的减毒沙门氏菌重组工程菌株及其应用

Resumen de: CN122214226A

本发明公开了一种htrA基因敲除的减毒沙门氏菌重组工程菌株及其应用，对减毒沙门氏菌VNP20009进行基因组遗传改造，将基因组中的htrA基因敲除，获得ΔhtrA‑VNP菌株。通过回补性表达带有/不带有信号肽的HtrA蛋白，首次证实信号肽对于HtrA蛋白的功能发挥至关重要。无信号肽的htrA基因回补菌株与ΔhtrA‑VNP菌株生物学表型无显著差异。本发明所述的表达带有信号肽的HtrA蛋白的ΔhtrA‑VNP菌株，HtrA蛋白通过调控细菌胞外多糖水平，一方面影响菌株的免疫原性与抗癌效果，另一方面影响菌株对抗巨噬细胞的杀伤效果，进而影响细菌在体内的分布特性。本发明基于htrA基因活性作用模式，建立了瘤内时空特异性启动表达带有信号肽的HtrA蛋白的ΔhtrA‑VNP菌株，实现更高效、安全与精准的活体微生物抗肿瘤治疗。

一种细菌复合疫苗与顺铂的协同抗肿瘤组合及其应用

Resumen de: CN122182751A

本发明公开了一种细菌复合疫苗与顺铂的协同抗肿瘤组合及其应用，属于肿瘤药物制备技术领域。所述抗肿瘤组合包括以下成份：细菌复合疫苗和顺铂。本发明通过细菌复合疫苗和顺铂的协同配合，在对肿瘤模型的试验小鼠进行治疗时发现，相较于单一的细菌复合疫苗治疗组和单一的顺铂治疗组，二者的协同用药对肿瘤细胞增殖的抑制作用显著上升，具有良好的抗肿瘤效果。

COMPOSITIONS AND METHODS FOR TREATMENT OF CANCER USING BACTERIA

Resumen de: US20260158079A1

Provided herein are compositions comprising substantially non-viable Gram-negative bacterial organisms that have a substantial reduction in endotoxin activity and/or pyrogenicity and methods for treating a cancer using the same. Also provided are methods for treating cancer provided herein, comprising administering to a mammal diagnosed with cancer, substantially non-viable Gram-negative bacteria having a substantial reduction in endotoxin activity and/or pyrogenicity, in an amount sufficient to inhibit growth or metastasis of the cancer. An additional method is provided comprising administering viable or non-viable Gram-negative bacterial organisms that have a genetic defect that results in a substantial loss of lipopolysaccharide within the outer membrane of the bacteria. Further provided are methods for reducing endotoxin activity and/or pyrogenicity in Gram-negative bacteria comprising treatment with polymyxin and glutaraldehyde.

DNA CONSTRUCT FOR EXPRESSING SIGNALING SUBSTANCE FOR CANCER TREATMENT, AND CANCER TREATMENT METHOD USING SAME

Resumen de: AU2024374864A1

Cyclic dinucleotides, which are a type of signaling substance for cancer treatment, are secondary messengers of intracellular events initiated by GPCR activation and act as a stimulator of interferon genes (STING), and thus can have a significant effect on tumor suppression. Meanwhile, when cancer occurs in a subject, angiogenesis and cell growth proceed at a very high rate in the body, and thus an oxygen-deficient environment is created due to incomplete angiogenesis in cancer tissues, which may be very suitable for the proliferation of anaerobic bacteria such as Salmonella sp. strains or E. coli. Therefore, the present invention relates to a DNA construct into which is introduced a gene for an enzyme that synthesizes a signaling substance for cancer treatment, e.g., a cyclic dinucleotide, or to a strain transformed with a vector comprising the DNA construct. The DNA construct or the strain transformed with a vector comprising the DNA construct, according to the present invention, targets cancer in a subject and then secretes C-di-AMP or C-di-GMP synthase in the surrounding environment of the cancer, and thus can very effectively prevent or treat cancer and, at the same time, can diagnose cancer in real time.

Bacillus subtilis as well as screening method and application thereof

Resumen de: CN122168470A

The invention discloses bacillus subtilis as well as a screening method and application thereof. The bacillus subtilis is named as bacillus subtilis BS61, is preserved in the China General Microbiological Culture Collection Center on September 4, 2025, and has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35815. The invention further discloses a preparation method of the bacillus subtilis. The screening method comprises phenotypic screening and genome screening, the phenotypic screening is to test the acid resistance, cholate resistance, high temperature resistance and artificial gastrointestinal fluid resistance of the strain, and reveal the acid resistance, cholate resistance, high temperature resistance and artificial gastrointestinal fluid resistance of the BS61 strain; the antibacterial effect on various pathogenic bacteria such as escherichia coli K88 and salmonella typhimurium is obviously superior to that of a bacillus subtilis standard strain BS168; according to the present invention, the low-abundance drug-resistant gene strain contains the bacteriostatic secondary metabolism gene clusters such as Fengycin, ThurincinH, Bacillus bactin and the like, does not have the aggressive virulence factor, and only contains three low-abundance drug-resistant genes; the compound can be applied to preparation of livestock and poultry feed additives, has no wide drug resistance risk, does not affect animal body health and food safety

Bacteriostatic white spirit vinasse containing bacillus zhangzhou GBW-NX as well as preparation method and application of bacteriostatic white spirit vinasse

Resumen de: CN122168441A

The invention discloses antibacterial distiller's grains containing bacillus Zhangzhou GBW-NX as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. The preservation number of the Zhangzhou bacillus GBW-NX is CGMCC (China General Microbiological Culture Collection Center) No.27402. Experiments prove that the Zhangzhou bacillus GBW-NX has the antibacterial effect of inhibiting escherichia coli, salmonella and staphylococcus aureus, and the Zhangzhou bacillus GBW-NX is put into a solid culture medium containing distiller's grains for fermentation and enzymolysis to prepare the antibacterial distiller's grains. The invention also proves that the bacteriostatic Baijiu vinasse can improve the intestinal functions of ruminants including lactation cows, increase the milk yield of the cows, broaden the application range of the Bacillus Zhangzhou GBW-NX, provide a new direction for feeding of the ruminants, and have important meanings for breeding of the ruminants, and the bacteriostatic Baijiu vinasse can be used for improving the intestinal functions of the ruminants including the lactation cows, improving the milk yield of the cows and widening the application range of the Bacillus Zhangzhou GBW-NX.

Biosensor for detecting salmonella typhimurium based on DNA nanowire activated CRISPR/Cas12a (clustered regularly interspaced short palindromic repeats/CRISPR/Cas12a) amplified by DNAzyme

Resumen de: CN122171800A

The invention belongs to the technical field of biological detection, and provides a biosensor for detecting salmonella typhimurium based on CRISPR/Cas12a activated by a DNA nanowire amplified by DNAzyme, and the biosensor comprises CRISPR/Cas12a protein, Mg < 2 + >, an aptamer Apt with a nucleotide sequence as shown in SEQ ID NO: 1-8, a chain A, a chain AB, a DNAzyme chain, a chain H1, a chain H2, a chain H3 and crRNA. The biosensor is high in detection sensitivity, low in detection limit, high in specificity, small in background interference, stable in fluorescence signal, simple to operate, mild in reaction condition, suitable for on-site rapid detection and suitable for actual food sample detection, the detection range can reach 10 <-10 > cfu/mL order of magnitude, the adding standard recovery rate is 100.3%-103.9%, and the detection cost is low. The method can be widely applied to salmonella typhimurium detection in the fields of food industry and public health.

Monoclonal antibody specific to Vibrio cholerae or Salmonella typhi and simultaneous rapid diagnostic kit using the same

Resumen de: KR20260084896A

본 발명은 콜레라균 또는 장티푸스균에 특이적인 단일클론 항체 및 이를 이용한 콜레라와 장티푸스 동시 신속진단키트에 관한 것으로, 보다 상세하게는 서열번호 2, 서열번호 4, 서열번호 6 또는 서열번호 8의 아미노산 서열로 표시되는 중쇄 가변영역을 포함하는 콜레라균(Vibrio cholerae)에 특이적으로 결합하는 단일클론 항체 및 서열번호 10 또는 서열번호 12의 아미노산 서열로 표시되는 중쇄 가변영역을 포함하는 살모넬라 티피(Salmonella typhi)에 특이적으로 결합하는 단일클론 항체를 이용한 콜레라와 장티푸스 동시 신속진단키트에 관한 것이다.

Induction method for improving activity of hermetia illucens antibacterial peptide

Resumen de: CN122139700A

The invention discloses an induction method for improving activity of hermetia illucens antibacterial peptide, and belongs to the technical field of insect biology. According to the method, Rhodopseudomonas palustris (RP) is added into strengthened basic ration of the fifth-instar larvae of hermetia illucens, and ultrasonic strengthening and short-time heat stress treatment are combined, so that efficient induction of the antibacterial peptide is realized. Test data show that the diameter of a salmonella inhibition zone of the crude extract of the antibacterial peptide induced by the method can reach 40.48 mm. The method solves the problem of large-scale production of antibacterial peptide induction, balances the immune cost caused by high-intensity induction by strengthening a substrate compensation mechanism, has the advantages of strong induction activity, stable growth performance, low process cost and the like, and has an application prospect in the fields of animal feed additives and biological bacteriostatic preparations.

Lactobacillus johnsonii capable of producing novel III-type bacteriocin, engineering bacteria and application of lactobacillus johnsonii

Resumen de: CN122146539A

The invention relates to lactobacillus johnsonii for producing novel III-type bacteriocin, engineering bacteria and application of the lactobacillus johnsonii and the engineering bacteria, and belongs to the technical field of microorganisms. The lactobacillus johnsonii JLJ071 strain is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the lactobacillus johnsonii JLJ071 strain is CGMCC No.34541. The lactobacillus johnsonii JLJ071 strain can be used for preparing the lactobacillus johnsonii strain. The amino acid sequence of the bacteriocin is as shown in Seq ID No. 1. The expressed novel III-type bacteriocin has good bactericidal activity on listeria monocytogenes and salmonella pullorum, has the characteristics of good acid resistance, high temperature resistance, protease resistance and the like, and has a good application prospect in feed additives.

Attenuated salmonella loaded adjuvant nano combined medicine as well as preparation method and application thereof

Resumen de: CN122140959A

The invention discloses an attenuated salmonella loaded adjuvant nano combined drug, which is characterized in that cyclodextrin is modified on the surface of bacteria by virtue of a condensation reaction of amino and carboxyl, and adamantane is modified on the surface of an adjuvant nano drug by virtue of a diselenide dynamic bond, so that the nano combined drug is prepared. Then adamantane and cyclodextrin are assembled through host-guest interaction of adamantane and cyclodextrin, so that the nano-drug is stably combined to the surface of attenuated salmonella. The diselenide dynamic bonds can be broken through ultrasonic response, so that the drug release effect is realized. The combined medicine has the tumor targeted enrichment capacity of bacteria and the characteristic that adjuvant nanoparticles are released under the action of ultrasonic waves, targeted delivery and deep penetration can be achieved in tumor tissue, and a remarkable tumor inhibition effect is achieved under stimulation of the ultrasonic waves. Experimental results show that the combined medicine shows a good treatment effect in the aspect of inhibiting tumor growth and has excellent biological safety at the same time.

Nano antibody Nb122 for resisting salmonella enteritidis as well as application and preparation method of nano antibody Nb122

Resumen de: CN122145623A

The invention relates to the technical field of molecular biology, in particular to an anti-salmonella enteritidis nano antibody Nb122 as well as application and a preparation method thereof, and relates to a nucleotide sequence and an amino acid sequence of the anti-salmonella enteritidis nano antibody Nb122. Meanwhile, the invention relates to application of the anti-salmonella enteritidis nano antibody Nb122 in preparation of a product for reducing adhesion and invasion of salmonella enteritidis to IPEC-J2 cells, and a preparation method of the anti-salmonella enteritidis nano antibody Nb122. According to the anti-salmonella enteritidis nano antibody provided by the technical scheme, the adhesion and invasion of salmonella enteritidis to IPEC-J2 cells can be reduced.

Independent electrochemical immunosensing device for instantly detecting salmonella typhimurium

Resumen de: CN122150572A

The invention discloses an independent electrochemical immunosensing device for instantly detecting salmonella typhimurium. The invention firstly provides a bendable patch. The bendable patch comprises a microfluidic module and a sensing module, the sensing module comprises a substrate and an immune sensing layer arranged on the substrate, and a working electrode, a reference electrode and a counter electrode are arranged on the immune sensing layer; the working electrode is obtained by sequentially modifying a carbon electrode with 3D-PCNAs, EDC/NHS, a salmonella typhimurium antibody and bovine serum albumin; the microfluidic module includes a sealing layer, a spacer layer, and an automatic transport layer. The free-standing electrochemical immunosensing device provided by the invention comprises a bendable patch and an electronic module. The electrochemical immunosensing device is independent working equipment, has the characteristics of no need of a peristaltic pump and low cost, and is suitable for on-site instant detection of salmonella typhimurium.

Methods of treatment of infections using bacteria

Resumen de: AU2026203814A1

The present disclosure relates generally to compositions, dosage forms, and methods for preventing and treating infections. The compositions include intact and substantially non- viable Gram-negative bacterial cells which have been treated to reduce lipopolysaccharide (LPS)-associated endotoxin activity, which surprisingly have increased activity to trigger immune cell production of cytokines. ay a y

Sodium caseinate-based Pickering emulsion as well as preparation method and application thereof

Resumen de: CN122124229A

The invention discloses a sodium caseinate-based Pickering emulsion as well as a preparation method and application thereof, and belongs to the technical field of vaccine adjuvants. The salmonella typhimurium flagellin and the sodium caseinate are used as raw materials to prepare the Pickering emulsion for the first time, and the prepared Pickering emulsion has the characteristics of small particle size, good biocompatibility and high stability, and can induce a body to generate higher immune response. The invention provides the application of the Pickering emulsion prepared by using salmonella typhimurium flagellin and sodium caseinate as raw materials as the vaccine adjuvant for the first time. The Pickering emulsion prepared by the preparation method disclosed by the invention is simple in steps, only needs two processes of mixing and homogenizing, and can effectively load a mode antigen OVA. The emulsion has good cell safety, and can promote endocytosis of macrophages to exogenous antigens. The emulsion can induce an organism to generate high-level antibody titer, and the antibody titer reaches more than 120,000 times.

LFS-SERS rapid quantitative detection method for salmonella

Resumen de: CN122128407A

The invention discloses an LFS-SERS rapid quantitative detection method for salmonella, and belongs to the technical field of food safety, the method comprises the following steps: S1, cracking a sample to be detected to obtain a crude DNA solution; s2, carrying out salmonella specific LAMP (loop-mediated isothermal amplification) by taking the crude DNA solution as a template, so as to obtain a biotin labeled product; and S3, mixing dCas9 protein, sgRNA, the SERS-labeled gold nanoparticle-probe conjugate, the biotin labeled product and a loading buffer solution, incubating at room temperature for 1-5 minutes, carrying out a chromogenic reaction through a lateral flow test strip, and carrying out SERS detection on a T line in LFS by using a portable Raman spectrometer connected with an intelligent terminal to realize quantitative detection of salmonella in a to-be-detected sample. The method is strong in specificity, high in sensitivity and good in stability, the whole detection process can be completed within 40 minutes, the cost is low, and the operation is simple and convenient.

Streptococcus nonlyticus and application of streptococcus nonlyticus in preparation of salmonella infection resistant preparation

Resumen de: CN122128190A

The invention relates to the technical field of microorganisms, in particular to streptococcus nonlyticus and application thereof in preparation of a salmonella infection resistant preparation. The invention provides a strain of streptococcus alactolyticus, which is preserved in the China Center for Type Culture Collection on March 30, 2026, and the preservation number of the streptococcus alactolyticus is CCTCC (China Center for Type Culture Collection) NO: M 2026565. After a host is subjected to intragastric administration of the streptococcus non-lactolyticus in advance, the resistance to salmonella typhimurium is obviously improved. After the host is infected with the salmonella typhimurium, the weight of the host is slowly reduced, the amount of the salmonella typhimurium in the liver and the spleen is inhibited, and the pathogenic bacterium load of the host is reduced.

Application of lactobacillus johnsonii cell-free supernatant in inhibition of salmonella biofilm

Resumen de: CN122123386A

The invention discloses application of a lactobacillus johnsonii cell-free supernatant in inhibition of a salmonella biofilm, and belongs to the technical field of microorganisms. The invention provides a new application of an acellular supernatant of lactobacillus johnsonii BNCC186110, and the acellular supernatant can inhibit the formation of biological membranes of salmonella typhimurium SL1344 and 14028, destroy the biological membranes of salmonella typhimurium SL1344 and 14028, and further reduce the colonization of salmonella typhimurium SL1344 and 14028.

Bacillus amyloliquefaciens capable of inhibiting bacteria and reducing ammonia nitrogen and application of bacillus amyloliquefaciens

Resumen de: CN122128143A

The invention belongs to the technical field of biology, and discloses bacillus amyloliquefaciens ZJ02 capable of inhibiting bacteria and reducing ammonia nitrogen, the preservation number is CCTCC NO: M20252399, the preservation date is October 30, 2025, the preservation unit is China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, China, the bacillus amyloliquefaciens ZJ02 has an obvious antibacterial effect on escherichia coli and salmonella, and the bacillus amyloliquefaciens ZJ02 can be used for preparing a bacillus amyloliquefaciens strain. The size of an inhibition zone of salmonella (SA) in 24 hours is 18.11 mm; compared with other strains, the strain has a better ammonia nitrogen degradation effect under the same condition, the ammonia nitrogen degradation rate is 76.58%, and when the initial ammonia nitrogen concentration is 500 mg/L, the ammonia nitrogen degradation rate reaches up to 87.57%; besides, the bacillus licheniformis has excellent capability of reducing ammonia release of animal waste, and in a pig farm environment ammonia removal capability test, compared with a control group, the NH3 concentration is reduced by 60.27% on the sixth day and is obviously superior to that of bacillus subtilis and bacillus licheniformis; meanwhile, the invention also provides application of the bacillus amyloliquefaciens.

METHODS AND SYSTEMS FOR THE RAPID DETECTION OF LISTERIA USING INFECTIOUS AGENTS

Nº publicación: MX2026005882A 01/06/2026

Solicitante:

LABORATORY CORP OF AMERICA HOLDINGS [US]
LABORATORY CORPORATION OF AMERICA HOLDINGS

Resumen de: MX2026005882A

Disclosed herein are methods and systems for rapid detection of microorganisms such as Listeria spp. in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as Listeria-specific bacteriophage, allows detection of a specific microorganism, such as Listeria spp. and an indicator signal may be amplified to optimize assay sensitivity.