Resumen de: CN121773219A
Described herein are compositions, methods, and kits for detecting diarrhea-causing pathogens from a patient, food, or environmental sample. One embodiment described herein is a primer pair and probe for a multiplex polymerase chain reaction (PCR)-based assay for the detection of diarrhea-causing pathogens, such as Campylobacter spp., Salmonella spp., Shigella spp./Enteroinvasive Escherichia coli (EIEC), Escherichia coli stx1/Shiga toxin A, or Escherichia coli stx2/Shiga toxin B. Other embodiments include methods and kits for detecting diarrhea-causing pathogens.
Resumen de: CN121762835A
The invention discloses an electrochemical sensor based on aptamer and bacteriophage dual recognition and application of the electrochemical sensor, and belongs to the technical field of food safety detection. The electrochemical sensor provided by the invention comprises a gold electrode modified by a DNA regular tetrahedron nanostructure and a calcined zeolite imidazate framework material anchored with phage and loaded with methylene blue, the DNA regular tetrahedron nanostructure comprises four DNA probes, and the four DNA probes are a chain A, a chain B, a chain C and a chain D; the bacteriophage is a salmonella typhimurium bacteriophage. The electrochemical sensor can perform dual recognition based on the aptamer and the bacteriophage, realizes high sensitivity, high specificity and rapid detection of salmonella typhimurium through the synergistic effect of all functional components, has biosafety prevention and control capability, and can meet the detection requirements of trace pathogenic bacteria in food.
Resumen de: CN121754522A
The invention discloses an application of gallic acid in preparation of a salmonella typhimurium CDSH inhibitor, and belongs to the technical field of biological medicine. CDSH enzyme activity inhibition experiment, H2S detection experiment, antibiotic stimulation experiment and greater wax moth infection model experiment analysis finds that the gallic acid can reduce the stress tolerance of S.Typhimurium by inhibiting the activity of CDSH enzyme, reduce the stress tolerance of S.Typhimurium, reduce the stress tolerance of S.Typhimurium, reduce the stress tolerance of S.Typhimurium, and reduce the stress tolerance of S.Typhimurium by inhibiting the activity of CDSH enzyme. The cysteine thiotransferase has the advantages that the cysteine thiotransferase can be used as a transferase inhibitor, the antibiotic sensitivity and the in-vivo removal effect of the cysteine thiotransferase are enhanced, the gallic acid is used for inhibiting the enzyme activity of CDSH and reducing the salmonella tolerance, and the cysteine thiotransferase inhibitor and the medicine for treating the caused infectious diseases are prepared and are used for preventing or treating salmonella typhimurium infection.
Resumen de: CN121762836A
The invention discloses a salmonella paratyphi A biosensor based on a nucleic acid aptamer and a preparation method of the salmonella paratyphi A biosensor. Three fluorine atoms (F) are introduced into the 5'end, the 3 'end and the middle position of an aptamer 2' F3-(5 '3'm DNA) sequence (SEQ ID NO: 1) for modification, so that the binding force of the aptamer and graphene is remarkably enhanced, the signal stability and the anti-interference capability of the biosensor are remarkably improved, non-specific fluorescence leakage can be effectively blocked, and reliable guarantee is provided for complex sample detection. According to the present invention, with the cooperation of the graphene oxide quenching substrate, the high sensitivity detection of the salmonella paratyphi A is achieved, the detection limit of the sensor within 5 min can achieve 10 cells/mL, the sensitivity is high, the specificity on the complex sample is more than 93.4%, and the method is suitable for food safety rapid screening and clinical diagnosis.
Resumen de: CN121754514A
The invention provides application of piceatannol in preparation of a salmonella flagellum inhibitor, and belongs to the technical field of biological medicines. Through analysis of salmonella sliding motion test, biofilm formation test, salmonella cell invasion test and other tests, it is found that piceatannol down-regulates flagellum-related gene transcription, weakens the sliding capacity, reduces biofilm formation and weakens the invasion capacity to host cells at non-inhibitory concentration; the invention relates to a salmonella flagellum inhibitor, particularly relates to a salmonella flagellum inhibitor, supports the development value of the salmonella flagellum inhibitor as an anti-virulence drug, provides a non-bactericidal treatment strategy capable of remarkably weakening bacterial pathogenicity, can be used as a potential lead compound or a composition therapy component for treating salmonella infection, and is applied to production and preparation of the salmonella flagellum inhibitor.
Nº publicación: CN121758606A 31/03/2026
Solicitante:
POULTRY INST SHANDONG ACADEMY OF AGRICULTURAL SCIENCE EXPERIMENTAL ANIMAL CENTER SHANDONG ACADEMY OF
\u5C71\u4E1C\u7701\u519C\u4E1A\u79D1\u5B66\u9662\u5BB6\u79BD\u7814\u7A76\u6240\uFF08\u5C71\u4E1C\u7701\u519C\u4E1A\u79D1\u5B66\u9662\u5B9E\u9A8C\u52A8\u7269\u4E2D\u5FC3\uFF09
Resumen de: CN121758606A
The invention relates to the technical field of poultry immunology, in particular to a universal riemerella anatipestifer monoclonal antibody, a detection kit and application of the universal riemerella anatipestifer monoclonal antibody. Specifically, the invention successfully designs the universal riemerella anatipestifer monoclonal antibody, and the detection kit developed based on the monoclonal antibody can rapidly detect the level of the riemerella anatipestifer serum antibody in a sample, and has high sensitivity and strong specificity. The riemerella anatipestifer strain has no cross reactivity with avian enterococcus faecalis, avian salmonella typhimurium, avian escherichia coli and avian salmonella enteritidis, so that the riemerella anatipestifer strain can be applied to rapid screening, epidemiological monitoring and vaccine immune effect evaluation of riemerella anatipestifer infection, and has a wide application prospect.