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Detection method and application of mouse pathogenic bacteria

Resumen de: CN121227913A

The invention relates to a multiplex fluorescent quantitative PCR (Polymerase Chain Reaction) method for detecting mouse pathogenic bacteria, which can be used for simultaneously detecting four pathogens, namely salmonella, corynebacterium murine, pseudomonas aeruginosa and klebsiella pneumoniae.

Application of peanut red skin procyanidine nano-liposome in inhibition of salmonella typhimurium

Resumen de: CN121220533A

The invention belongs to the technical field of food safety, and relates to application of peanut red skin proanthocyanidin nano-liposome in inhibition of salmonella typhimurium, and the peanut red skin proanthocyanidin nano-liposome causes cell death by causing permeability change of cell membranes and cell walls of salmonella typhimurium. According to the present invention, the inherent limitation of the natural antibacterial substance PSPc is overcome through the efficient delivery system of the nano-liposome, such that the salmonella typhimurium inhibition activity of the nano-liposome can be completely performed and durably maintained, the method is efficient and safe, the application potential in the food preservation aspect is provided, and the value-added utilization of the peanut resource is improved.

一种将猪霍乱沙门氏菌进行低内毒化改造的方法及其应用

Resumen de: CN121227763A

一种将猪霍乱沙门氏菌进行低内毒化改造的方法及其应用，它涉及疫苗领域，本发明通过基因工程技术对猪霍乱沙门氏菌弱毒株SC014的lipid A修饰，对猪霍乱沙门氏菌进行低内毒素化改造，成功构建了低内毒素的猪霍乱沙门氏菌突变体。改造后的猪霍乱沙门氏菌及其外膜囊泡在显著降低内毒素活性的同时，仍能有效激活免疫反应，实现了安全性与有效性的兼顾，为开发新型疫苗提供了新的思路和方法。

Method for detecting mouse salmonella by using qPCR (quantitative polymerase chain reaction) technology instead of traditional sacrificial sentinel mouse

Resumen de: CN121227909A

The invention discloses a method for detecting mouse salmonella by using a qPCR (quantitative polymerase chain reaction) technology instead of a traditional sacrificial sentinel mouse, and a salmonella specific primer and probe combination comprises a forward primer with a sequence of 5 '-AGCGTACTGGAAAGGGAAAG-3', as shown in SEQ ID NO.1, a reverse primer with a sequence of 5 '-AGCGTACGAAAG-3', as shown in SEQ ID NO.2, a reverse primer with a sequence of 5 '-AGCGTACGAAGGAAAG-3', as shown in SEQ ID NO.3, the sequence of a reverse primer is 5-ATACCGCCAATAAAGTTCACAAAG-3 ', and the sequence of the reverse primer is as shown in SEQ ID NO. 2; the probe sequence of the probe is 5 '-FAM-CGTCACCTTGATAAACTCTATGCA-BHQ1-3', and the probe sequence of the probe is as shown in SEQ ID NO. 3. The method has the beneficial effects that a real-time fluorescent quantitative PCR method is established by utilizing the specific primer and the double-labeled fluorescent probe, so that rapid detection of salmonella pollution in facilities is realized; according to the method based on the environmental sample, use of additional animals is avoided, operation is easy and convenient, the result is accurate, and sensitivity and specificity are both superior to those of a traditional sentinel mouse method.

Bacteria-enzyme synergistic product for bacteriostasis, odor removal and deodorization of pets as well as preparation method and application of bacterium-enzyme synergistic product

Resumen de: CN121221502A

The invention provides a bacterium-enzyme synergistic product for bacteriostasis, odor removal and deodorization of pets as well as a preparation method and application of the bacterium-enzyme synergistic product. The active ingredients of the product comprise a compound bacteria solution and a compound enzyme preparation, the composite bacterial liquid is prepared by compounding bacterial liquids of bacillus subtilis, bacillus amyloliquefaciens, lactobacillus acidophilus, rhodopseudomonas palustris and saccharomyces cerevisiae according to a specific volume ratio; the compound enzyme preparation is prepared by compounding protease, lipase, glucose oxidase and laccase according to a specific weight ratio. Through the synergistic effect of bacteria and enzymes, organic matters in pet excrement can be quickly decomposed, malodorous gases such as ammonia gas and hydrogen sulfide are eliminated from the source, and breeding of harmful bacteria such as staphylococcus aureus and salmonella is effectively inhibited. The product is safe, non-toxic, non-irritant and efficient and lasting in effect, can be used for spraying treatment on the body surface of a pet or the living environment of the pet, and solves the problems that a traditional physical or chemical deodorization method is short in effect and irritant.

Lactobacillus paracasei with effect of relieving inflammatory bowel disease and application of lactobacillus paracasei

Resumen de: CN121227591A

The invention discloses a lactobacillus paracasei strain with an effect of relieving inflammatory bowel diseases and application thereof, and belongs to the technical field of microbial fermentation. The strain is Lactobacillus paracasei JGSLP08, and the preservation number of the Lactobacillus paracasei JGSLP08 is CGMCC (China General Microbiological Culture Collection Center) NO.34274. The invention further discloses a preparation method of the Lactobacillus paracasei JGSLP08. The strain has the characteristics of strong acid resistance, bile salt resistance and gastrointestinal fluid resistance, can effectively inhibit salmonella typhimurium, escherichia coli and staphylococcus aureus, and has an excellent antioxidant effect. A DSS-induced colitis mouse model shows that the lactobacillus paracasei can effectively improve the DIA score of a mouse, regulate inflammatory factors, reduce oxidative stress injury, relieve inflammatory cell infiltration and protect the integrity of mucosal epithelium, so that the inflammatory bowel disease is relieved and/or treated.

Edible fungus solid-state fermentation feed additive based on medicinal plant forestry and agricultural residue compound matrix and preparation method of edible fungus solid-state fermentation feed additive

Resumen de: CN121196071A

The invention discloses an edible fungus solid-state fermentation feed additive based on a medicinal plant forestry and agricultural residue compound matrix and a preparation method thereof, and belongs to the technical field of microbial fermentation and feed additives, the feed additive is prepared by performing bidirectional solid-state fermentation on edible fungi by utilizing straws and a medicinal plant compound matrix, the content of antibacterial peptide is 0.8 g/kg to 3.5 g/kg, and the content of the antibacterial peptide is 0.5 g/kg to 1.5 g/kg. The straw-medicinal plant composite substrate has the advantages that the straw-medicinal plant composite substrate has the adenosine content of 0.3 g/kg to 1.8 g/kg, the crude protein content of 18% to 35% and the total polysaccharide content of 12% to 28%, and has remarkable bacteriostatic activity on escherichia coli, staphylococcus aureus and salmonella, compared with the prior art, the straw-medicinal plant composite substrate directionally induces a fungal metabolic pathway through the synergistic effect of the straw and the medicinal plant composite substrate, the content of functional active substances is greatly increased, and the application prospect is broad. The antibacterial, bacteriostatic and immune regulation functions are remarkably enhanced, the raw material cost is reduced by 60-75%, and the feed additive can be widely applied to livestock and poultry breeding as a green antibiotic replacement product.

Novel broad-spectrum antibacterial peptide YHX-9 and application thereof

Resumen de: CN121202967A

The invention belongs to the technical field of biology, and relates to a novel broad-spectrum antibacterial peptide YHX-9 and application thereof. The amino acid sequence of the antibacterial peptide YHX-9 is as shown in SEQ ID No.1, and the antibacterial peptide YHX-9 is short in synthetic sequence, low in chemical synthesis difficulty and suitable for large-scale production; the antibacterial peptide YHX-9 has good antibacterial activity on staphylococcus aureus, listeria monocytogenes, streptococcus mutans, escherichia coli, salmonella typhimurium and pseudomonas aeruginosa, and has wide application prospects in the fields of food or medicines, cosmetics, agriculture, animal husbandry and the like.

Genetically engineered bacterium, medicine, fungicide, application of genetically engineered bacterium, medicine and fungicide and method for constructing genetically engineered bacterium

Resumen de: CN121203928A

The invention relates to the technical field of bioengineering, and discloses a genetically engineered bacterium, a medicine, a fungicide, application of the genetically engineered bacterium and a method for constructing the genetically engineered bacterium, the genetically engineered bacterium is attenuated salmonella typhimurium carrying plasmids, the plasmids are plasmids cloned with genes of GLP-1 analogues, and the GLP-1 analogues are 4 * GLP-1 and/or GLP-1-Fc; the amino acid sequence of the 4 * GLP-1 is as shown in SEQ ID NO. 1; the amino acid sequence of the GLP-1-Fc is as shown in SEQ ID NO. 2. The genetically engineered bacterium provided by the invention has the effects of reducing blood sugar, reducing blood fat, relieving fatty degeneration, reducing pancreatic tissue damage and/or liver tissue damage, reducing inflammatory cell infiltration, promoting insulin secretion of pancreatic beta cells, reducing lipid accumulation in liver tissues and the like, and has the effect of treating colon cancer. Therefore, the genetically engineered bacterium disclosed by the invention can be used as a medicine for preventing and/or treating the metabolic syndrome.

Antibacterial peptide pig NK lysin mutant and application thereof

Resumen de: CN121202989A

The invention relates to the technical field of agricultural biology, in particular to an antibacterial peptide pig NK lysin mutant and application thereof. The amino acid of each alpha helix of the antibacterial peptide, especially the composition and position of basic amino acid and hydrophobic amino acid, is subjected to combined mutation, so that the antibacterial peptide with an enhanced antibacterial function is screened. The antibacterial activity of the pig NK lysin mutant disclosed by the invention on different bacteria, such as staphylococcus aureus, salmonella typhimurium, escherichia coli and salmonella pullorum, is enhanced.

Alpha spiral mutant of granulysin with improved antibacterial activity and application of alpha spiral mutant

Resumen de: CN121202988A

The invention relates to the technical field of agricultural biology, in particular to an alpha spiral mutant of granulysin with improved antibacterial activity and application of the alpha spiral mutant. The amino acid sequence of the alpha spiral mutant peptide of granulysin with improved antibacterial activity is as shown in SEQ ID No: 2. According to the present invention, the antibacterial activity of the granulysin mutant on different bacteria such as gram-negative bacteria salmonella typhimurium, escherichia coli and salmonella pullorum is enhanced;

SUBSTITUTED 2-AMINOBENZIMIDAZOLES ANALOGS AS ANTIBIOFILM AGENTS

Resumen de: US2025387373A1

In one aspect, the disclosure relates to compositions and methods for dispersing exiting Salmonella biofilms and inhibiting formation of Salmonella biofilms. In various aspects, the disclosed compositions can be used in methods of treating a persistent Salmonella infection, including an asymptomatic infection. Such infections can colonize a variety of tissues, including the gall-bladder. Also disclosed are methods of treating typhoid fever. Also disclosed are methods for mitigating or preventing secondary outbreaks of typhoid fever by treating asymptomatic subjects who had been symptomatic for typhoid fever at a previous time. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

VACCINE COMPOSITION COMPRISING A SYSTEM FOR DELIVERING AN INACTIVATED WHOLE BACTERIUM VIA CATIONIC POLYSACCHARIDE NANOPARTICLES WITHOUT ANY ADJUVANT

Resumen de: CN120712079A

The present invention relates to the field of vaccine compositions. The invention more particularly relates to a prophylactic vaccine composition comprising killed intact bacteria intended for use in mammals and birds, said bacteria being wrapped with a cationic agent, in particular cationic nanoparticles.

Antibacterial peptide for inhibiting food-borne pathogens as well as acquisition method and application of antibacterial peptide

Resumen de: CN121181665A

The invention provides an antibacterial peptide for inhibiting food-borne pathogens as well as an acquisition method and application of the antibacterial peptide, and relates to the technical field of bioactive peptides and biomedical engineering. The amino acid sequence of the antibacterial peptide is as follows: Gly-Leu-Thr-Leu-Lys-His-Leu-Lys-Lys-Leu-Phe, and the antibacterial peptide can be used for effectively inhibiting the growth of food-borne pathogens such as staphylococcus aureus, salmonella, escherichia coli, pseudomonas aeruginosa, listeria monocytogenes and the like. The defects in the prior art are overcome, the antibacterial peptide with antibacterial activity is found and identified through a screening method combining a gastrointestinal digestive enzyme-peptide omics technology and an experiment, and a new natural antibacterial agent is provided for food, medicine and other related fields.

Primer group for simultaneously detecting four pathogenic microorganisms and application of primer group in preparation of micro-fluidic chip and kit

Resumen de: CN121182993A

The invention discloses a primer group for simultaneously detecting four pathogenic microorganisms and application of the primer group in preparation of a micro-fluidic chip and a kit. The sequences of the primers are as shown in SEQ ID NO: 1-24. The method has the advantages that the microfluidic technology and the isothermal amplification technology are combined, four sample indexes are detected at the same time in one-time detection, and the method has the advantages of being high in throughput, high in specificity, good in experiment repeatability and convenient and rapid to operate; the kit can be used for rapidly and accurately detecting salmonella (SAL), staphylococcus aureus (SA), listeria monocytogenes (LM) and escherichia coli O157: H7 (E. coli O157: H7).

System and method for risk assessment of animal population human-livestock disease

Resumen de: CN121195273A

Systems and methods for assessing the risk of a human-livestock disease, including salmonella, in an animal population at a production facility. A user may provide data in response to a plurality of data entry fields, which may relate, at least in part, to processes, operations, and/or devices at a production facility. For each data entry field, a value may be assigned to the data provided by the user. The value may correspond to a predetermined value of a predetermined response identified as corresponding to the user input data. The assigned value may be adjusted by a weighting factor, which may correspond to a predicted impact that the action or information reflected by the data input by the user may have on the prevention of zoonosis. The assigned and/or adjusted values may be compiled to generate a risk assessment score, which may be used to determine a food safety index score and/or a food safety plan.

Composite preparation containing bovine lactoferrin and lyase and application thereof

Resumen de: CN121154798A

The invention discloses a compound preparation containing bovine lactoferrin and lyase and application of the compound preparation, and belongs to the technical field of biology. The preparation is composed of bovine lactoferrin and streptococcus lyase which are both prepared through a baculovirus-insect cell eukaryotic expression system. Researches show that the compound preparation has a remarkable synergistic antibacterial effect. The streptococcus lyase not only can effectively inhibit various gram-positive bacteria, but also can overcome the limitation that single streptococcus lyase has weak activity on gram-positive bacteria, and obviously enhances the inhibition effect on escherichia coli and salmonella. Particularly, the preparation still has definite in-vitro bacteriostatic activity on multi-drug-resistant escherichia coli such as ciprofloxacin and doxycycline, and a new way is provided for developing novel drugs for resisting drug-resistant bacteria.

Salmonella low water activity tolerance prediction method, device and system, and storage medium

Resumen de: CN121171360A

The invention discloses a salmonella low water activity tolerance prediction method, device and system and a storage medium, and the method comprises the steps: collecting salmonella genome data in a public database, and recording the water activity information of a source; processing the genome data to form a data matrix; performing redundancy elimination operation on the data matrix; dividing the data matrix; respectively carrying out model training through three machine learning algorithms by using the data matrix; obtaining an optimal performance model based on a Bayesian parameter adjustment method; and the low water activity tolerance of the salmonella is judged based on prediction of three algorithm models. By adopting the technical scheme provided by the invention, the problems of insufficient prediction coverage caused by dependence on a single gene marker and overfitting caused by high whole genome feature redundancy in the prior art are solved.

PRIMER AND PROBE FOR DETECTING SALMONELLA PULLORUM, AND KIT

Resumen de: WO2025255902A1

A primer and probe for detecting Salmonella pullorum, and a kit. The primer comprises SP-F and SP-R for detecting the target sequence of the Salmonella pullorum (SP) citE2 gene, and the probe is modified with a fluorescent reporter group and a fluorescent quenching group. By means of providing the primer pair of SP-F and SP-R, which pair specifically binds to the target sequence of the citE2 gene, and combining the primer pair with the probe, whic is used for specific recognition, the accurate and sensitive detection of Salmonella pullorum can be realized.

ANTI-BACTERIAL PROTEIN COMPLEX

Resumen de: MX2025009214A

The invention relates to protein bacteriocins (PBs) as therapeutic agents, and specifically to protein complexes comprising two or more PB molecules associated with a protein scaffold which comprises cognate immunity protein domains for the effector portions of the respective PBs. In particular, the invention provides an anti-bacterial protein complex comprising (a) a first PB molecule and a second PB molecule; and (b) an immunity protein scaffold comprising a first immunity protein domain and a second immunity protein domain; wherein the first and second immunity protein domains are non-covalently bound to the respective first and second PB molecules.

ADI Attenuated Salmonella Gallinarum expressing ADI and uses thereof

Resumen de: KR20250175381A

본 발명은 항암 물질인 아르기닌 디이미나아제 (arginine deiminase; ADI)를 발현하는 항종양 박테리아 균주 및 이의 용도에 관한 것으로, 본 발명에 따른 항암 물질 생산 시스템이 도입된 면역 활성능이 있는 살모넬라 균주는 우수한 면역 항암 효능을 나타내어, 기존 항암제와 함께 또는 독립적으로 암의 치료제로 사용될 수 있다.

Rapid immunochromatography detection kit for gastrointestinal pathogenic bacteria based on viral nano-enzyme probe

Resumen de: CN121142029A

The invention belongs to the technical field of biological detection, and discloses a rapid immunochromatography detection kit for gastrointestinal pathogenic bacteria based on a viral nano-enzyme probe. The invention discloses a nano-enzyme probe, the virus-shaped nano-enzyme probe provided by the invention takes Fe3O4 nano-particles as a core, Pt nano-particles and Au (at) Pt nano-particles are coated in sequence, a specific antibody is modified, and the nano-enzyme probe has a magnetic enrichment effect and a multi-catalytic-site characteristic at the same time; rapid capture and high-sensitivity quantitative detection of various gastrointestinal pathogenic bacteria in complex matrixes (river water samples, lake water samples and excrement samples) can be realized, and matrix interference in practical application is eliminated; and the kit has huge potential in the aspects of high-sensitivity field screening and clinical monitoring of infection of gastrointestinal bacteria (such as escherichia coli, salmonella and helicobacter pylori).

Application of traditional Chinese medicine composition in preparation of medicine for preventing and/or treating bacterial infectious diarrhea

Resumen de: CN121130030A

The invention belongs to the technical field of medicines, and particularly relates to application of a traditional Chinese medicine composition in preparation of a medicine for preventing and/or treating bacterial infectious diarrhea. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 30 to 60 parts of rhizoma coptidis, 30 to 60 parts of cortex magnoliae officinalis, 5 to 20 parts of radix aucklandiae and 5 to 20 parts of rhizoma zingiberis. The bacterial infectious diarrhea is bacterial infectious diarrhea caused by salmonella. The traditional Chinese medicine composition prepared by the invention can obviously improve the diarrhea condition of mice, improve the weight loss condition of the mice, reduce the inflammation level and relieve the lesion degree of intestinal tissues.

Bian chicken source bacillus licheniformis and application thereof

Resumen de: CN121136879A

The invention discloses Bian chicken source bacillus licheniformis and application thereof, and belongs to the technical field of microorganisms, the Bian chicken source bacillus licheniformis is named as bacillus licheniformis YYBJ-06-01 and preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC No.M 20252095. The Bian chicken source bacillus licheniformis is named as bacillus licheniformis YYBJ-06-01, and the Bian chicken source bacillus licheniformis is named as bacillus licheniformis YYBJ-06-01 and preserved in the China Center for Type Culture Collection (CCTCC). The Bian chicken source bacillus licheniformis can significantly enhance the immunity of the organism, increase the content of serum immune globulin IgG and reduce the level of inflammatory factors. Meanwhile, the serum antioxidant enzyme activity can be effectively improved, the malondialdehyde content is reduced, and the antioxidant function of the body is enhanced. Besides, the Bian chicken source bacillus licheniformis has a definite inhibition effect on common pathogenic bacteria such as escherichia coli, salmonella and the like, is sensitive to most of common antibiotics and high in biological safety, and provides an excellent strain resource for developing a green feed additive for replacing the antibiotics.

Application of ramoplanin as antibacterial synergist of polymyxin antibiotics

Nº publicación: CN121130046A 16/12/2025

Solicitante:

SOUTH CHINA AGRICULTURAL UNIV
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Resumen de: CN121130046A

The invention belongs to the technical field of antibacterial synergists, and discloses application of ramoplanin as a colistin antibacterial synergist. The invention provides a treatment strategy for combined use of ramoplanin as a polymyxin drug sensitizer in treatment of gram-negative bacterial infection, and in a test of combined use of ramoplanin and colistin for detection of a bacterial sterilization curve and the integrity of a bacterial outer membrane, the concentration of the ramoplanin is low and is only 16 mu g/mL; in the aspect of the antibacterial effect, the FICI index is relatively low, and after the ramoplanin and the colistin are jointly used, the MIC (Minimum Inhibitory Concentration) of the colistin can be reduced by 16 times at most. The research shows that ramoplanin is a promising antibiotic sensitizer, and can be used in combination with the polymyxin drug colistin to resist the colistin drug resistance problem of escherichia coli, salmonella and klebsiella pneumoniae.