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Phytobacterium parabarium LN43 and application thereof

Resumen de: CN120966710A

The invention discloses a strain of lactobacillus parabarium LN43 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the lactobacillus parabarium LN43 is GDMCC No.66615, the preservation unit is Guangdong Province Microbial Culture Collection Center, the preservation time is June 30, 2025, and the address of the preservation unit is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou City. The lactobacillus parabarium LN43 has the advantages that the lactobacillus parabarium LN43 has the preservation number of GDMCC No.66615, the preservation number is GDMCC No.66615, the preservation unit is Guangdong Province, the preservation time is June 30, 2025, and the preservation unit is the June 30, 2025. The lactobacillus parabarium LN43 has relatively high acid resistance and a certain cholate tolerance. The lactobacillus parabarium LN43 has the advantages of high acid resistance and certain cholate tolerance. The strain has a very strong inhibition effect on pathogenic microorganisms such as staphylococcus aureus, salmonella pullorum, salmonella enteritidis, escherichia coli, pseudomonas aeruginosa, salmonella typhimurium, micrococcus luteus, acinetobacter baumannii and klebsiella pneumoniae, and is a broad-spectrum antibacterial microorganism.

Weissella fusiformis JWC120 and application thereof

Resumen de: CN120966694A

The invention relates to the technical field of microorganisms, in particular to Weissella fusiformis JWC120 with a function of inhibiting common harmful bacteria in animal breeding and application of the Weissella fusiformis JWC120. The Weissella fusiformis JWC120 with the function of inhibiting common harmful bacteria in animal breeding, provided by the invention, has the preservation number of CGMCC No.31945, and can effectively inhibit common escherichia coli, salmonella, staphylococcus aureus and shigella in animal breeding; a bacteriocin crude extract of the strain has a remarkable inhibition effect on common staphylococcus aureus in animal breeding, and has a good application prospect in animal disease prevention and treatment and development of livestock and poultry breeding health-care products.

Plant additive of piglet feed, piglet feed, piglet milk powder material and application

Resumen de: CN120937995A

The embodiment of the invention provides a plant additive of a piglet feed, the piglet feed, a piglet milk powder material and application. According to the plant additive for the piglet feed, antibacterial components of the plant additive are composed of eucommia ulmoides extract and orange peel extract, and the mass ratio of the eucommia ulmoides extract to the orange peel extract is (2.0-3.0): (1.0-1.5). By adopting the compatibility of the folium cortex eucommiae extract and the orange peel extract, the reproduction of coliform and salmonella as pathogenic bacteria in the intestinal tracts of the piglets can be effectively inhibited, and the plant additive has anti-inflammatory and antibacterial effects on the intestinal tracts of the piglets, so that the intestinal health of the piglets can be maintained, and the absorption of the piglets on nutrient substances of the feed after eating is ensured; the survival rate and the weaning weight of the piglets are favorably improved.

Salmonella colorimetric detection method based on DNAWalker driving double-signal amplification

Resumen de: CN120945079A

The invention develops a colorimetric detection method for salmonella based on DNAWalker driving double signal amplification. The detection principle is as follows: target DNA triggers AP hybridization to form Y-type connection with CP, an Nt.BbvCI restriction enzyme cutting site is exposed, DNAWalker is released through restriction enzyme cutting circulation, and target circulation amplification is realized. Meanwhile, PDANS (palladium-iron-nano particles) is synthesized through Fe < 2 + >/H2O2 Fenton reaction, a pH indicator is loaded through silver nano particle modification (PDANS (at) Ag), and a signal is enhanced. An amplified product is combined with PDANS (at) Ag through a magnetic bead sandwich structure, remarkable color change is caused, and quantitative detection can be performed through a smart phone App (such as' Color Grab ') or a microwell plate reader. The method is easy and convenient to operate, high in sensitivity and suitable for on-site rapid identification of salmonella.

アセトキシ水素化ホウ素類を用いた糖抗原のコンジュゲーション

Resumen de: CN120418260A

The present disclosure generally relates to improved methods for preparing glycoconjugates. The method includes conjugating a saccharide to a carrier protein using an acetoxyborohydride-containing reducing mixture prepared in situ.

Beef cattle feed additive and preparation method thereof

Resumen de: CN120937994A

The invention provides a beef cattle feed additive and a preparation method thereof, and belongs to the technical field of feed additives.Eucommia ulmoides fermentation extract is added to the feed additive, contains a large amount of chlorogenic acid and has a synergistic effect with citrus flavone, the content of fat in beef muscles is remarkably increased, nano-coated slow-release selenium yeast is added, the selenium bioavailability is increased, and the feed additive has the advantages of being capable of improving the beef quality and the like. Meanwhile, the citrus flavonoid microcapsules are added, so that the release of the citrus flavonoid microcapsules in rumen is reduced, the citrus flavonoid microcapsules are released in a targeted manner in the abomasum, and the utilization rate of citrus flavonoid is increased. The probiotics and the traditional Chinese medicine mixture are combined to inhibit escherichia coli and salmonella, and the illness probability of beef cattle is reduced. In the raw materials, through the synergistic effect of the composite mineral substances and the traditional Chinese medicine components, the anti-stress capability of the beef cattle is improved.

Antibacterial peptide p-1-SB and application thereof

Resumen de: CN120943899A

The invention belongs to the technical field of antibacterial products, and relates to an antibacterial peptide p-1-SB and application thereof. The amino acid sequence of the antibacterial peptide p-1-SB is as shown in SEQ ID NO.1, the antibacterial peptide p-1-SB is screened from a probiotic fungus Saccharomyces boulardii, and the antibacterial peptide p-1-SB has the advantages of low hemolysis, low cytotoxicity, high stability and efficient in-vivo antibacterial activity, and has application safety. The antibacterial peptide p-1-SB has strong antibacterial activity on Gram-negative bacteria enterohemorrhagic escherichia coli, urinary tract infection escherichia coli, shigella, salmonella and acinetobacter baumannii which are commonly infected by a human body, and has potential application value in development of prevention and treatment of bacterial infections such as intestinal diarrhea or urinary tract infection.

DETECTION OF PATHOGENIC E. COLI STRAINS

Resumen de: WO2025233323A1

The invention relates to compositions of binding peptides, and individual binding peptides, capable of specific binding to either of two variants of OmpA occurring in pathogenic E. coli, and to methods employing these binding peptides for detection or isolation of pathogenic E. coli.

ENGINEERED IMMUNOSTIMULATORY BACTERIAL STRAINS AND USES THEREOF

Resumen de: US2025345369A1

Provided are delivery immunostimulatory bacteria that have enhanced colonization of tumors, the tumor microenvironment and/or tumor-resident immune cells, and enhanced anti-tumor activity. The immunostimulatory bacteria are modified by deletion of genes encoding the flagella, or by modification of the genes so that functional flagella are not produced, and/or are modified by deletion of pagP or modification of pagP to produce inactive PagP product. As a result, the immunostimulatory bacteria are flagellin and/or pagP−. The immunostimulatory bacteria optionally have additional genomic modifications so that the bacteria are adenosine or purine auxotrophs. The bacteria optionally are one or more of asd−, purI−, and msbB−. The immunostimulatory bacteria, such as Salmonella species, are modified to encode immunostimulatory proteins that confer anti-tumor activity in the tumor microenvironment, and/or are modified so that the bacteria preferentially infect immune cells in the tumor microenvironment, or tumor-resident immune cells, and/or are modified to induce less cell death in immune cells than in other cells. Also provided are methods of inhibiting the growth or reducing the volume of a solid tumor by administering the immunostimulatory bacteria.

항-박테리아 단백질 복합체

Resumen de: MX2025009214A

The invention relates to protein bacteriocins (PBs) as therapeutic agents, and specifically to protein complexes comprising two or more PB molecules associated with a protein scaffold which comprises cognate immunity protein domains for the effector portions of the respective PBs. In particular, the invention provides an anti-bacterial protein complex comprising (a) a first PB molecule and a second PB molecule; and (b) an immunity protein scaffold comprising a first immunity protein domain and a second immunity protein domain; wherein the first and second immunity protein domains are non-covalently bound to the respective first and second PB molecules.

一种马链球菌和马流产沙门菌二联融合抗原及应用

Resumen de: CN120924562A

本发明公开了一种马链球菌和马流产沙门菌二联融合抗原，其核苷酸序列如SEQ ID NO.1所示。本发明将马流产沙门菌的FljB与马链球菌的SeM、EAG、GAPDH部分抗原串联重组在一起，不仅可以同时预防马链球菌和马流产沙门菌2种不同的马传染病，还增强了小鼠的体液免疫应答水平，利用该融合抗原免疫可获得抵抗马链球菌和马流产沙门菌的攻毒保护效果，高于单独抗原蛋白的免疫和灭活全菌的免疫，这解决了以往灭活疫苗免疫效率低和免疫繁琐的问题，有利于降低免疫的成本。

Antibacterial compound, preparation method and application thereof, and pesticide agent

Resumen de: CN120923334A

The invention relates to the technical field of natural antibacterial compounds, in particular to an antibacterial compound, a preparation method and application thereof and a pesticide agent. The antibacterial compound is selected from compounds shown in the following structural formula: the compound has an inhibition effect on methicillin-resistant staphylococcus aureus and other drug-resistant bacteria, staphylococcus aureus, escherichia coli, salmonella, pseudomonas aeruginosa and other bacteria, and alternaria, fusarium, colletotrichum and other fungi; the compound can be applied as broad-spectrum antibacterial, broad-spectrum antifungal and drug-resistant bacterium resistant drugs.

Microflora for treating industrial wastewater

Resumen de: CN120924423A

The invention discloses a flora for treating industrial wastewater and application of the flora for treating the industrial wastewater or polluted soil, the flora comprises stenotrophomonas and at least two selected from the group consisting of comamonas, ochrobactrum, pseudomonas, brucella, citrobacter, salmonella, balloon and enterococcus, and wherein the industrial wastewater contains a bactericide.

Chicken preservation method based on compound essential oil extracted from natural plants

Resumen de: CN120918228A

The invention provides a chicken preservation method based on compound essential oil extracted from natural plants. The preparation method comprises the following steps: selecting thyme leaves, clove buds and cortex cinnamomi as raw materials, and fermenting by utilizing lactobacillus plantarum and saccharomyces cerevisiae to obtain fermented essential oil; uniformly mixing and stirring the fermented essential oil, rosemary essential oil and tea tree essential oil to form compound essential oil; preparing a nano emulsion; clathration is performed to obtain microencapsulated fermentation essential oil; and uniformly spraying the microencapsulated essential oil emulsion on the surface of the chicken, performing vacuum packaging, and performing refrigeration at 4 DEG C. According to the invention, the plant essential oil is modified through microbial fermentation, and a nano slow-release technology is combined, so that an efficient, safe and lasting fresh-keeping effect is realized, the active ingredients of the compound essential oil are increased after fermentation, the inhibition effect on common food-borne pathogenic bacteria such as salmonella and staphylococcus aureus is remarkably improved, and the refrigeration and fresh-keeping period of chicken is prolonged to 21 days or more.

抗细菌蛋白质复合物

Resumen de: MX2025009214A

The invention relates to protein bacteriocins (PBs) as therapeutic agents, and specifically to protein complexes comprising two or more PB molecules associated with a protein scaffold which comprises cognate immunity protein domains for the effector portions of the respective PBs. In particular, the invention provides an anti-bacterial protein complex comprising (a) a first PB molecule and a second PB molecule; and (b) an immunity protein scaffold comprising a first immunity protein domain and a second immunity protein domain; wherein the first and second immunity protein domains are non-covalently bound to the respective first and second PB molecules.

Antibacterial peptide chicken NK lysin mutant and application thereof

Resumen de: CN120904309A

The invention relates to the technical field of agricultural biology, in particular to an antibacterial peptide chicken NK lysin mutant and application thereof. According to the present invention, the antibacterial activity of the chicken NK lysin mutant on different bacteria such as salmonella typhimurium 541, gram-negative bacteria, Escherichia coli O157: H7 1491, gram-negative bacteria, salmonella pullorum of gram-negative bacteria, and Staphylococcus aureus can be enhanced, and the antibacterial activity of the chicken NK lysin mutant on different bacteria such as salmonella typhimurium 541, gram-negative bacteria, Escherichia coli O157: H7 1491, gram-negative bacteria, salmonella pullorum of gram-negative bacteria, and Staphylococcus aureus can be enhanced;

Anaerobic bacteria co-culture device and application

Resumen de: CN120904995A

The invention discloses an anaerobic bacterium co-culture device and application thereof.The anaerobic bacterium co-culture device comprises a first screw injector 1 and a second screw injector 8, the first screw injector 1 is sequentially connected with a first Luer external thread adapter 2, a first hose 3, a filter membrane clamp 4, a second hose 5, a second Luer external thread adapter 6 and the second screw injector 8, and the first Luer external thread adapter 2, the first hose 3, the filter membrane clamp 4, the second hose 5, the second Luer external thread adapter 6 and the second screw injector 8 are sequentially connected; and a filter membrane 7 is arranged in the filter membrane clamp 4. The anaerobic bacteria co-culture device is simple in structure, low in cost and convenient to use, can easily simulate an anaerobic environment and co-culture anaerobic bacteria without anaerobic culture equipment, can well culture two different species of bacteria in the same liquid environment without cross contamination, is suitable for co-culture of various common anaerobic bacteria, and has a wide application prospect. Such as salmonella typhimurium, bifidobacterium breve, fecal bacillus przewalskii, ackermania or prevotella. The method has great benefits for the initial research of related research projects of bacterial interaction.

PHAGE LYASE AND USE THEREOF

Resumen de: WO2025227308A1

A phage lyase with an amino acid sequence set forth in SEQ IN NO.2. The lyase can inhibit the growth of Staphylococcus, Escherichia, Klebsiella, and Salmonella bacteria, and can be used as an antibacterial substance in milk pollution control, belonging to the field of bioengineering. The phage lyase can be used for preparing an enzyme formulation alone or in a compounded manner to specifically inactivate bacteria such as Staphylococcus aureus, thereby providing an enzyme formulation source which is safe and free of toxic and side effects for controlling Staphylococcus aureus pollution in milk at present.

弱毒化サルモネラ・チフィムリウム（Ｓａｌｍｏｎｅｌｌａ ｔｙｐｈｉｍｕｒｉｕｍ）を使用する良性神経系腫瘍の処置

Resumen de: US2022125906A1

Compositions and methods for the treatment of benign nervous system tumors including schwannomas using attenuated Salmonella typhimurium and optionally one or more checkpoint inhibitors.

PCR (Polymerase Chain Reaction) detection kit for rapidly identifying salmonella gallinarum with pullorum disease

Resumen de: CN120888393A

The invention relates to the technical field of pullorum disease, in particular to a PCR (Polymerase Chain Reaction) detection kit for quickly identifying salmonella gallinarum with pullorum disease, which comprises a detection system and a shell, and a top cover is hinged to one side of the top of the shell; a loading plate is vertically arranged in the shell in a sliding fit manner, a plurality of loading ports for loading PCR tubes are formed in the loading plate, and loading bags are fixedly connected to the loading ports; an oscillating assembly used for oscillating the loading bag and a limiting assembly used for limiting the position of the loading plate are arranged in the shell. The oscillation assembly comprises an oscillation plate, a controller and a driving part, and the controller is used for controlling the driving part to operate; the oscillating plate is located below the loading bag and is in vertical sliding fit with the inner side wall of the shell; the driving piece is fixedly connected to the inner bottom wall of the shell, and an output shaft of the driving piece is fixedly connected with a stirring wheel. Through the synergistic effect, the oscillation plate can achieve the efficient oscillation effect, it is ensured that reactants are evenly distributed in a PCR tube, and the uniformity and efficiency of the PCR reaction are improved.

Application of lactobacillus paracasei Jlus 660321 in bacteriostasis

Resumen de: CN120884615A

The invention belongs to the field of microorganisms, and particularly relates to application of lactobacillus paracasei Jlus 660321 in the aspect of bacteriostasis, the lactobacillus paracasei Jlus 660321 is classified and named as lactobacillus paracasei, is preserved in the China General Microbiological Culture Collection Center (CGMCC), has the preservation number of CGMCC No.33962, is separated and screened from naturally fermented dairy products, and has the advantages that the lactobacillus paracasei Jlus 660321 can be used for preparing the lactobacillus paracasei; then, after ultraviolet radiation mutagenesis, a series of screening experiments are adopted to obtain the strain. The strains inhibited by the strain comprise bacteria and fungi, and the bacteria comprise one or more of escherichia coli, staphylococcus aureus and salmonella. The fungi comprise one or more of botrytis cinerea, trichothecium roseum, aspergillus niger, fusarium verticillium, Penicillium Polish, Penicillium adalidaea and Penicillium oxalicum; the method can be used for preparing products such as antibacterial drugs, food and health-care products, and has wide development prospects and application values.

Probiotic product for inhibiting salmonella in chicken body and screening method

Resumen de: CN120888460A

The invention relates to the technical field of microorganisms, in particular to a probiotic product for inhibiting salmonella in a chicken body and a screening method, the probiotic product comprises Chencity bacillus sachariensis, and the colony count of the Chencity bacillus sachariensis in the probiotic product is 107-1010 CFU/mL. Based on the screening method of the probiotics for inhibiting the salmonella in the chicken body, the screened city bacillus sachariensis is prepared into the probiotic product for inhibiting the salmonella in the chicken body, so that the salmonella infection condition of the chicken can be effectively improved, the weight of the chicken with positive salmonella infection is increased, and the salmonella in the chicken body is inhibited. The salmonella loading quantity in chicken bodies is obviously reduced, the salmonella loading quantity has the characteristics of low residual quantity and environment friendliness, and the problems of antibiotic residues, bacterial drug resistance increase and the like caused by antibiotic abuse can be avoided. By adopting the screening method of the probiotics for inhibiting the salmonella in the chicken body, provided by the invention, the dominant probiotics capable of inhibiting the salmonella can be simply, conveniently and efficiently determined.

Salmonella typhimurium sensor for regulating CRISPR/Cas12a activity based on DNAzyme conformation conversion and application

Resumen de: CN120888680A

The invention provides a salmonella typhimurium sensor for regulating and controlling CRISPR (clustered regularly interspaced short palindromic repeats)/Cas12a (CRISPR/Cas12a) activity on the basis of DNAzyme conformation conversion and The biosensor comprises an Apt-T composite probe, a hairpin D chain, a hairpin TS chain, crRNA, Cas12a, Mg < 2 + > and FQ. The Apt-T composite probe is obtained by hybridizing Apt and a T chain; and the 44th site of the TS chain is ribonucleotide. The biosensor provided by the invention is low in detection limit, and high-specificity detection of a target object is realized by virtue of specific recognition of the nucleic acid aptamer and combination of the aptamer and salmonella typhimurium. The detection limit of the biosensor is superior to that of most existing methods; and the background signal of the detection sample is also reduced. The sensor has the advantages of high detection speed, simplicity in operation, low detection limit, high specificity and the like, can make up the defects and deficiencies of the existing salmonella typhimurium detection, and realizes rapid and accurate quantitative detection of salmonella typhimurium.

CRISPR/Cas12a-responsive AIE MOF nano-enzyme hydrogel dual-mode biosensor as well as construction method and application of CRISPR/Cas12a-responsive AIE MOF nano-enzyme hydrogel dual-mode biosensor

Resumen de: CN120888643A

The invention discloses a CRISPR/Cas12a responsive AIE MOF nano-enzyme hydrogel dual-mode biosensor as well as a construction method and application thereof, and belongs to the technical field of food safety rapid detection. In order to solve the technical problems that an existing salmonella detection method is tedious in operation, low in detection speed, dependent on special equipment and professional operators, prone to being polluted by aerosol, high in price and the like, a difunctional AIE MOF nano material with AIE fluorescence performance and POD activity is packaged into DNA hydrogel and is further combined with a CRISPR/Cas12a system, and the detection sensitivity of salmonella is improved. A biosensor for detecting salmonella is constructed, and a high-sensitivity, accurate and rapid method is provided for detecting salmonella in food safety.

Rutin and fangchinoline synergistic compound preparation for resisting salmonella infection and application

Nº publicación: CN120884601A 04/11/2025

Solicitante:

JILIN UNIV
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Resumen de: CN120884601A

The invention relates to a compound preparation for treating salmonella infection, which comprises rutin and fangchinoline, and the concentration ratio of the fangchinoline to the rutin is 1: 2. The compound preparation plays an antibacterial role through an anti-virulence strategy and a host-oriented strategy, remarkably reduces the bacteria loading amount of salmonella in tissue organs and intestinal contents of mouse and chick models, improves tissue pathological damage, and has good safety. The compound preparation disclosed by the invention provides an efficient and safe novel scheme for treating salmonella infection.