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Constant-temperature rapid detection method for multiple nucleic acids of diarrheogenic escherichia coli

Resumen de: CN121874375A

The invention discloses a constant-temperature rapid detection method for multiple nucleic acids of diarrheogenic Escherichia coli, and belongs to the technical field of rapid detection of microorganisms. A primer probe group is designed according to specific virulence genes of diarrheogenic Escherichia coli, and a quadruple nucleic acid constant-temperature rapid detection system aiming at escV, bfpB, aggR and invE genes, a triple nucleic acid constant-temperature rapid detection system aiming at stx1, stx2 and pic genes and a triple nucleic acid constant-temperature rapid detection system aiming at It, sth and astA genes are established; the kit can be used for detecting diarrheagenic Escherichia coli EPEC, EHEC, ETEC, EAEC and EIEC, and the purpose of detecting the diarrheagenic Escherichia coli rapidly and conveniently is achieved. The technology consumes less time, can react at normal temperature, reduces dependence on professional instruments, can be applied to daily laboratory detection to shorten time, can also be applied to field on-site rapid diagnosis, and provides a new technical means for detection of diarrheogenic escherichia coli.

Escherichia coli mutant strain with high yield of L-cysteine and application of escherichia coli mutant strain

Resumen de: CN121852296A

The invention provides an Escherichia coli mutant strain with high yield of L-cysteine and application, and belongs to the technical field of bioengineering, the strain is named as MFAPA45QT, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO: 31970. According to the method, molecular modification means such as metabolic engineering and the like are combined with a traditional breeding method, and through mutation sites existing in 3-phosphoglycerate dehydrogenase and serine acetyltransferase, feedback regulation on key enzymes in the L-cysteine synthesis process is effectively relieved, and the yield of L-cysteine is increased; the escherichia coli mutant strain MFAPA45QT is obtained by carrying out ARTP mutagenesis coupling L-cysteine specific response biosensor high-throughput screening, the L-cysteine yield in a fermentation tank level can reach 31.11 g/L, and the method is suitable for industrial production of L-cysteine.

Method for detecting Escherichia coli in food by combining Fe3O4 (at) Mo-CDs nano-enzyme with aptamer

Resumen de: CN121831141A

The invention discloses a method for detecting Escherichia coli in food by combining Fe3O4 (at) Mo-CDs nano-enzyme with an aptamer, according to the method, near-infrared light response molybdenum-doped carbon dot modified Fe3O4 (at) Mo-CDs nano-enzyme is synthesized through a hydrothermal method, the Fe3O4 (at) Mo-CDs nano-enzyme has excellent NIR enhanced peroxidase activity and magnetism, 3, 3 ', 5, 5'-tetramethyl benzidine is oxidized to generate blue oxidized TMB, and the activity and magnetism of the NIR enhanced peroxidase are improved. After the surface of the Fe3O4 (at) Mo-CDs nano-enzyme is functionalized with an escherichia coli nucleic acid aptamer, the POD-like activity of the Fe3O4 (at) Mo-CDs nano-enzyme is inhibited, when Escherichia coli exists, the Fe3O4 (at) Mo-CDs nano-enzyme can be selectively combined with E.coli, the POD-like activity of the nano-enzyme is further inhibited, when the Fe3O4 (at) Mo-CDs nano-enzyme is used for detecting the E.coli in samples such as fruit juice and cakes, the detection limit is as low as 0.767 CFU/mL, and the Fe3O4 (at) Mo-CDs nano-enzyme has relatively high reliability and accuracy and can be widely applied to detection of E.coli. According to the invention, magnetic targeting separation and visual detection of E.coli are realized.

Primer group and kit for detecting avian pathogenic escherichia coli based on LAMP (Loop-Mediated Isothermal Amplification) method

Resumen de: CN121826198A

The invention discloses a primer group and a kit for detecting avian pathogenic escherichia coli based on an LAMP (Loop-Mediated Isothermal Amplification) method, and belongs to the technical field of biological detection. The primer group comprises an upstream outer primer F3, a downstream outer primer B3, an upstream inner primer FIP and a downstream inner primer BIP, the kit comprises the primer group: an upstream outer primer F3, a downstream outer primer B3, an upstream inner primer FIP and a downstream inner primer BIP. The novel avian pathogenic Escherichia coli LAMP primer group is autonomously designed, is used for detecting avian pathogenic Escherichia coli based on a loop-mediated isothermal amplification method and can be applied to kit detection, a result can be obtained by observing color change, and a large amount of time is saved; meanwhile, the lowest detection limit of target strains reaches 3.6 * 100 CFU/mL, and the detection limit is remarkably improved compared with that of a traditional PCR method.

Primer and probe set for detecting sheep abortion-causing bacteria and application of primer and probe set

Resumen de: CN121826192A

The invention provides a primer and a probe group for detecting sheep abortion-causing bacteria and application of the primer and the probe group, and belongs to the technical field of biological detection. The primer and probe group is characterized in that the sequence of the primer and probe group for detecting the listeria is shown as SEQ ID NO.1-3, and the sequence of the probe group for detecting the listeria is shown as SEQ ID NO.2-3; according to the primer for detecting the salmonella abortus, the sequence of the probe group is as shown in SEQ ID NO.4-6; the sequences of the primer and the probe group aiming at Brucella detection are shown as SEQ ID NO.7-9; the sequence of a primer and a probe group for detecting chlamydia psittaci is as shown in SEQ ID NO.10-12. The invention further discloses a kit for detecting chlamydia psittaci. The method has the characteristics of visualization, high sensitivity, strong specificity, simplicity and convenience in operation, short time consumption and the like, realizes rapid differential diagnosis of four sheep bacterial abortion diseases through one-time PCR amplification, and has important advantages in the field of sheep disease diagnosis and identification.

Strain identification and drug sensitivity detection kit as well as preparation method and application thereof

Resumen de: CN121801685A

The invention belongs to the technical field of strain identification and drug sensitivity detection, and discloses a strain identification and drug sensitivity detection kit and a preparation method and application thereof, the kit comprises a drug sensitivity disc, a pre-enrichment chromogenic culture medium, a chromogenic culture medium, sterile water and a diluent; the drug sensitive disc body comprises an enterobacteriaceae area, an enterococcus area, a staphylococcus area, a pseudomonas aeruginosa area and an acinetobacter baumannii area; the enterobacteriaceae area is provided with enterobacteriaceae identification culture medium micropores and drug micropores, the enterococcus area is provided with enterococcus identification culture medium micropores and drug micropores, the staphylococcus area is provided with staphylococcus identification culture medium micropores and drug micropores, and the pseudomonas aeruginosa area is provided with pseudomonas aeruginosa identification culture medium micropores and drug micropores. An acinetobacter baumannii identification culture medium micropore and a medicine micropore are matched in the acinetobacter baumannii area; the problems that an existing drug sensitivity test mode is complex in operation, high in cost and low in accuracy of identification and detection results are solved.

Composite material GZHMU-2 (at) Ag and application of composite material GZHMU-2 (at) Ag in preparation of self-cleaning biosensor

Resumen de: CN121797397A

The invention belongs to the technical field of organic materials, and particularly relates to a composite material GZHMU-2 (at) Ag and application of the composite material GZHMU-2 (at) Ag in preparation of a self-cleaning biosensor. The composite material GZHMU-2 (at) Ag is synthesized by loading AgNPs on GZHMU-2 through a method of reducing silver nitrate through ultraviolet irradiation, and the GZHMU-2 (at) Ag can generate active oxygen through photocatalysis, so that an antibacterial effect is achieved, and the composite material has a killing effect on escherichia coli and staphylococcus aureus. Based on a metal covalent organic framework modified by silver nanoparticles, a carboxyl functionalized SA31 aptamer is fixed on GZHMU-2 (at) Ag through an amidation reaction, a self-cleaning electrochemical biosensor for sensitive detection and real-time inactivation of bacteria is developed, the photocatalytic and electrochemical properties of the sensor are remarkably enhanced, and the sensor has a wide application prospect. The method has important potential for field monitoring and photocatalytic disinfection of pathogens in practical application.

Reusable dual-mode aptamer biosensor for detecting clostridium perfringens

Resumen de: CN121805581A

The invention discloses a reusable dual-mode aptamer biosensor for detecting clostridium perfringens, which is characterized by comprising a competitive chain CP-C, a CeO2 (at) PANI nano composite material, a closed bipolar electrode system and an aptamer functionalized magnetic bead probe. According to the invention, the high-affinity nucleic acid aptamer is combined with the specific extracellular electron transfer mechanism of the clostridium perfringens viable bacteria for the first time, a new viable bacteria detection sensing strategy free of nucleic acid extraction and target gene amplification is constructed, and the biosensor can specifically recognize the viable clostridium perfringens, and can be used for detecting the viable bacteria of the clostridium perfringens. According to the present invention, with the detection method, the false positive result caused by the existence of dead bacteria is avoided, the detection can be rapidly completed within 3 min, the voltage signal and the G value signal are mutually proved, the false positive or false negative of the single signal detection is effectively avoided, the detection limit is as low as 2.50 CFU/mL, and the excellent anti-interference ability in the complex food matrix such as chicken and milk is provided; the core element ITO glass electrode can be continuously and repeatedly used for 12 times, and the performance is not obviously attenuated.

Oligonucleotide composition for female lower genital tract pathogen detection, kit and application

Resumen de: CN121780729A

The invention relates to the technical field of biological detection, and discloses an oligonucleotide composition for detecting pathogens in a female lower genital tract, a kit and application. The oligonucleotide composition disclosed by the invention can be used for rapidly and simultaneously detecting a plurality of pathogens in gardnerella vaginalis, atriurella vaginalis, prevotella vulgaris, campylobacter mobilis, bacteroides, trichomonas vaginalis and mycoplasma in a sample in a high-specificity manner, and has high sensitivity and high stability; and a rapid, non-invasive, accurate and convenient detection method is provided for female reproductive health detection.

Double-antibody sandwich ELISA detection kit for detecting Escherichia coli BL21 LPS and application thereof

Resumen de: CN121784290A

The invention provides a double-antibody sandwich ELISA detection kit for detecting Escherichia coli BL21 LPS and application thereof, the kit comprises: an ELISA plate coated with a capture antibody, the capture antibody being a mouse polyclonal antibody against Escherichia coli BL21 LPS; the detection antibody is an anti-escherichia coli BL21 LPS (Lipopolysaccharide) rabbit polyclonal antibody; the enzyme-labeled secondary antibody is specifically combined with the detection antibody, and the enzyme-labeled secondary antibody is an HRP labeled goat anti-rabbit IgG enzyme-labeled antibody; and an Escherichia coli BL21 LPS antigen. The kit can specifically recognize LPS from Escherichia coli BL21, effectively eliminate interference of other Gram-negative bacteria LPS, realize accurate detection of target residues, provide a reliable detection means for LPS residue control of fermentation broth, intermediate products and final products in the production process of biological drugs, directly guarantee the quality safety of the biological drugs, and has a wide application prospect. The medication risk caused by LPS residues is reduced, and the health rights and interests of patients are protected.

Detection reagent for detecting bacterial cfDNA by using digital PCR, detection method and application

Resumen de: CN121780686A

The invention relates to a detection reagent and a detection method for detecting bacterial cfDNA by using digital PCR and application, a bacterial cfDNA extraction reagent and a digital PCR amplification reaction reagent are adopted as the detection reagent, primers and probe compositions for detecting the bacterial cfDNA in the digital PCR amplification reaction reagent are optimized, the primers have nucleotide sequences as shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 7 and SEQ ID NO: 8, and the probe compositions have nucleotide sequences as shown in SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 7 and SEQ ID NO: 8. The probe has nucleotide sequences as shown in SEQ ID NO: 3, SEQ ID NO: 6 and SEQ ID NO: 9, cfDNA of enterotoxin-producing bacteroides fragilis, fusobacterium nucleatum and pks + escherichia coli is subjected to qualitative detection and quantitative analysis through digital PCR, the detection method is simple, high in sensitivity, high in precision and good in stability, and the probe has important application value in detection of cfDNA of bacteria and preparation of colorectal cancer screening and early diagnosis reagents.

ZIF-8 (at) AuNPs-CRISPR/Cas12a colorimetric biosensor based on assistance of smart phone and application of ZIF-8 (at) AuNPs-CRISPR/Cas12a colorimetric biosensor

Resumen de: CN121780736A

The invention discloses a ZIF-8 (at) AuNPs-CRISPR/Cas12a colorimetric biosensor based on assistance of a smart phone and application of the ZIF-8 (at) AuNPs-CRISPR/Cas12a colorimetric biosensor, a novel CRISPR/Cas12a biosensing platform is constructed based on a metal organic framework (MOF) nano enzyme (namely ZIF-8 (at) AuNPs), and the novel CRISPR/Cas12a colorimetric biosensor is used for detecting salmonella typhimurium. In the system, a CRISPR (clustered regularly interspaced short palindromic repeats) system is used as a target recognition and signal transduction module, and a ZIF-8 (at) AuNPs nano composite material with high porosity and oxidase-like activity is used as a colorimetric signal amplification module, so that the CRISPR/Cas12a-ZIF-8 (at) AuNPs colorimetric biosensor is constructed. The sensor shows an excellent linear relation to salmonella typhimurium, and the detection limit is as low as 11 CFU/mL. By establishing a CRISPR colorimetric biosensor platform assisted by a smartphone, salmonella typhimurium detection without instrument assistance is realized, and the huge potential of carrying out hyperstable field detection on food-borne pathogenic bacteria in a food supply chain is shown.

Nucleic acid aptamer-based salmonella paratyphi A biosensor and preparation method thereof

Resumen de: CN121762836A

The invention discloses a salmonella paratyphi A biosensor based on a nucleic acid aptamer and a preparation method of the salmonella paratyphi A biosensor. Three fluorine atoms (F) are introduced into the 5'end, the 3 'end and the middle position of an aptamer 2' F3-(5 '3'm DNA) sequence (SEQ ID NO: 1) for modification, so that the binding force of the aptamer and graphene is remarkably enhanced, the signal stability and the anti-interference capability of the biosensor are remarkably improved, non-specific fluorescence leakage can be effectively blocked, and reliable guarantee is provided for complex sample detection. According to the present invention, with the cooperation of the graphene oxide quenching substrate, the high sensitivity detection of the salmonella paratyphi A is achieved, the detection limit of the sensor within 5 min can achieve 10 cells/mL, the sensitivity is high, the specificity on the complex sample is more than 93.4%, and the method is suitable for food safety rapid screening and clinical diagnosis.

Listeria monocytogenes RAA-CRISPR/Cas12a detection method based on one-tube one-step method

Resumen de: CN121759623A

The invention relates to the technical field of listeria monocytogenes detection, in particular to a listeria monocytogenes RAACRISPR/Cas12a detection method based on a one-tube one-step method. Specifically, the invention provides a novel listeria monocytogenes detection method, which is based on construction of an optimized one-tube one-step RAA-CRISPR/Cas detection system, reduces mutual interference of two reaction systems by optimizing the addition proportion of the two reaction systems, and further improves the detection sensitivity of the listeria monocytogenes by optimizing the composition of components of the RAA-CRISPR/Cas reaction systems. Comprising a buffer solution, an RAA primer, a probe, Cas12a and the like in concentration, so that the detection sensitivity and specificity are greatly improved while one-tube one-step rapid detection is realized, and a rapid and efficient listeria monocytogenes detection method with ultrahigh sensitivity and specificity is obtained.

Carbapenem drug resistance gene detection primer group based on multiple PCR-time-of-flight mass spectrometry, detection kit and kit use method

Resumen de: CN121759621A

The invention discloses a carbapenem drug resistance gene detection primer group based on multiple PCR-time-of-flight mass spectrometry, a detection kit and a kit using method, and through a large number of screening and verification experiments, a multiple primer group suitable for time-of-flight mass spectrometry detection is obtained. Therefore, the multi-PCR-time-of-flight mass spectrometry detection of the multi-drug-resistant genes of carbapenem-resistant enterobacteriaceae bacteria becomes possible. The detection kit prepared by adopting the primer group can be used for simultaneously detecting and identifying 10 common carbapenem drug-resistant genes, including klebsiella pneumoniae KPC gene, NDM gene, IMP gene, VIM gene and OXA gene, acinetobacter baumannii OXA gene, NDM gene, Escherichia coli OXA gene, IMP gene and serratia marcescens VIM gene. Meanwhile, the kit is high in sensitivity and specificity, dozens of times of detection can be carried out in the same batch, so that the detection efficiency is greatly improved, and the kit is suitable for rapid screening and genetic typing of carbapenem drug-resistant bacteria in clinical samples.

Universal riemerella anatipestifer monoclonal antibody, detection kit and application of universal riemerella anatipestifer monoclonal antibody

Resumen de: CN121758606A

The invention relates to the technical field of poultry immunology, in particular to a universal riemerella anatipestifer monoclonal antibody, a detection kit and application of the universal riemerella anatipestifer monoclonal antibody. Specifically, the invention successfully designs the universal riemerella anatipestifer monoclonal antibody, and the detection kit developed based on the monoclonal antibody can rapidly detect the level of the riemerella anatipestifer serum antibody in a sample, and has high sensitivity and strong specificity. The riemerella anatipestifer strain has no cross reactivity with avian enterococcus faecalis, avian salmonella typhimurium, avian escherichia coli and avian salmonella enteritidis, so that the riemerella anatipestifer strain can be applied to rapid screening, epidemiological monitoring and vaccine immune effect evaluation of riemerella anatipestifer infection, and has a wide application prospect.

Method and device for detecting pathogenic bacteria

Resumen de: CN121759580A

The invention discloses a pathogenic bacterium detection method and device, and belongs to the technical field of pathogenic bacterium detection. The detection method of the pathogenic bacteria comprises the following steps: constructing a liquid drop micro-fluidic chip CRISPR/Cas reaction system: forming a water-in-oil liquid drop by a micro-fluidic chip, wherein the liquid drop contains an aptamer-block DNA double strand, a CRISPR/Cas system and a signal probe containing a labeling element; pathogenic bacteria identification and signal activation: adding a sample to be detected into the reaction system, specifically binding the pathogenic bacteria with the aptamer, and cutting the signal probe to release the labeled element; a plasma mass spectrum single-particle mode is used for detecting labeled elements, and single-bacterium level detection of pathogenic bacteria is achieved. In addition, the invention also provides a detection device for pathogenic bacteria, which comprises a micro-fluidic chip, a liquid transmission unit and a plasma mass spectrum. The detection method provided by the invention meets the low-concentration requirement of pathogenic bacteria detection, and realizes high-sensitivity pathogenic bacteria detection.

Multiplex detection combination for detection of gastrointestinal bacterial nucleic acids

Resumen de: CN121773219A

Described herein are compositions, methods, and kits for detecting diarrhea-causing pathogens from a patient, food, or environmental sample. One embodiment described herein is a primer pair and probe for a multiplex polymerase chain reaction (PCR)-based assay for the detection of diarrhea-causing pathogens, such as Campylobacter spp., Salmonella spp., Shigella spp./Enteroinvasive Escherichia coli (EIEC), Escherichia coli stx1/Shiga toxin A, or Escherichia coli stx2/Shiga toxin B. Other embodiments include methods and kits for detecting diarrhea-causing pathogens.

LAMP (Loop-Mediated Isothermal Amplification) method high-throughput detection primer group and kit for five food-borne pathogenic bacteria and application thereof

Resumen de: CN121737326A

The invention discloses five food-borne pathogenic bacteria LAMP method high-throughput detection primer groups, a kit and application, and belongs to the technical field of food-borne pathogenic bacteria detection. The primer group corresponds to five bacteria, namely salmonella, vibrio parahaemolyticus, listeria monocytogenes, escherichia coli O157: H7 and staphylococcus aureus, and the nucleotide sequences of the primers are SEQ ID No.1-30. A matched system can complete the whole-process detection at the constant temperature of 62 DEG C for 45 minutes, the result does not need a complicated instrument, the positive grey and negative purple are judged by naked eyes, and the operation is simple and convenient; the detection sensitivity reaches 1-75 copies/reaction, an artificially polluted aquatic product sample does not need to be subjected to enrichment and is subjected to simple splitting decomposition and enrichment, the specificity is high, and no cross reaction exists; the anti-interference capability is outstanding, the method adapts to complex matrixes of aquatic products, and the detection coincidence rate is high; the device is simple in structure and low in cost, only needs a conventional constant temperature device, is suitable for basic food, agriculture, disease control, customs and other detection institutions, food enterprises, on-site law enforcement and on-site detection and other scenes, and provides an efficient and economic technical means for aquatic pr

Primer group for detecting common pneumonia pathogenic bacteria of forest musk deer and application of primer group

Resumen de: CN121737324A

The invention relates to the technical field of medical detection, in particular to a primer group for detecting common pneumonia pathogenic bacteria of forest musk deer and application of the primer group. The invention provides a primer group for detecting common pneumonia pathogenic bacteria (escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa and pasteurella multocida) of forest musk deer, and successfully establishes a multiplex PCR (Polymerase Chain Reaction) method capable of simultaneously amplifying four pathogenic bacteria through a polymerase chain reaction principle. The method is high in specificity; the kit is high in sensitivity, and in a PCR reaction system of 25 microliters, the detection limit of escherichia coli is 9.2 * 10 < 4 > CFU/mL, the detection limit of klebsiella pneumoniae is 1.3 * 10 < 5 > CFU/mL, the detection limit of pseudomonas aeruginosa is 1.5 * 10 < 5 > CFU/mL, and the detection limit of pasteurella multocida is 3.7 * 10 < 4 > CFU/mL; clinical tests show that the method is good in repeatability.

Matrix type photoelectric sensing array-based pathogenic bacteria culture test detection system and pathogenic bacteria growth state judgment method

Resumen de: CN121736869A

The invention discloses a pathogenic bacteria culture test detection system based on a matrix type photoelectric sensing array and a pathogenic bacteria growth state judgment method thereof, and relates to the field of medical examination equipment. The system comprises a bearing device, a constant-temperature culture module, a swing mixing mechanism, a matrix type photoelectric sensing array and a control module, wherein each culture test tube corresponds to a plurality of transmission light detection positions on the side and a reflected light detection position at the bottom; the pathogen growth state determination method comprises the following steps: acquiring light intensity data every 10 minutes to form a continuous data curve, and analyzing and determining whether the pathogen grows or not, the surface/precipitation/turbidity growth state and the growth rate. The invention overcomes the defects of high cost, large volume, low flux and only qualitative determination in the prior art, realizes automatic, high-flux, digital and accurate detection, reduces the risk of biological pollution, and is suitable for clinical examination and quarantine institutions.

Method and device for detecting types of pathogenic bacteria in meat products

Resumen de: CN121746777A

The invention provides a meat pathogenic bacterium type detection method and device, and relates to the technical field of hyperspectral reconstruction.The method comprises the steps that on the basis of an RGB image of a to-be-detected sample of a target meat type and a pre-trained RGB-hyperspectral data reconstruction model, full-wave-band hyperspectral data of the to-be-detected sample is reconstructed; preprocessing the reconstructed full-band hyperspectral data of the to-be-detected sample; determining a characteristic wave band of the to-be-detected sample based on the preprocessed full-wave band hyperspectral data by adopting a characteristic wave band selection algorithm; extracting a characteristic spectrum corresponding to a characteristic wave band from the preprocessed full-wave band hyperspectral data; and inputting the meat type and the characteristic spectrum of the to-be-detected sample into a pre-trained pathogenic bacterium classification model, and outputting a pathogenic bacterium type classification result of the to-be-detected sample. Based on the technical scheme, the accuracy of identifying the types of pathogenic bacteria infected by meat products can be improved.

Integrated nano platform for bimodal detection and synergistic sterilization of pathogenic bacteria as well as preparation method and application of integrated nano platform

Resumen de: CN121720929A

The invention discloses an integrated nano platform for bimodal detection and synergistic sterilization of pathogenic bacteria as well as a preparation method and application of the integrated nano platform, and belongs to the technical field of food safety. The platform is composed of a multifunctional nano probe (MPDA/Pd SA-DA-CDs/Apt) and a magnetic separation assembly (MNRs/PGA/AM). The probe integrates enzyme-like catalysis and photo-thermal performance and is used for generating colorimetric and photo-thermal dual-mode signals so as to realize high-sensitivity and high-reliability detection of pathogenic bacteria. The magnetic separation assembly is used for specifically capturing and enriching target bacteria and eliminating matrix interference. After detection is positive, the platform can immediately start secondary propulsion synergistic sterilization: firstly, the action distance is shortened through magnetic aggregation, and then pathogenic bacteria are efficiently inactivated and biological membranes are disintegrated by utilizing the synergistic effect of near-infrared laser excitation photothermal effect and active oxygen. According to the invention, the integration of detection and disinfection is realized, the operation is convenient, and the method has important application value in the fields of food safety and biomedicine.

Double probe for detecting salmonella as well as preparation method and application of double probe

Resumen de: CN121718608A

The invention provides a double probe for detecting salmonella as well as a preparation method and application of the double probe, and belongs to the technical field of bacterial detection. The double probes provided by the invention comprise an aptamer-phage-magnetic bead probe MB (at) Phage (at) Apt and an fDNA-gold nanoparticle probe AuNPs (at) fDNA; the sequence of the aptamer is as shown in SEQ ID NO: 1, and the sequence of the fDNA is as shown in SEQ ID NO: 2. The double probes are assisted by an ultraviolet lamp, on-site rapid visual detection of salmonella is realized through system color change caused by change of fluorescence intensity, the provided detection method is simple to operate, low in cost, less in time consumption and easy to realize, and on-site rapid visual detection of salmonella can be realized without large experimental equipment; a data analysis system and a linear equation are further utilized for calculation, the content of salmonella can be detected in a short time, and the detection efficiency and accuracy are improved.

Escherichia coli virulence gene and drug-resistant gene detection primer group based on time-of-flight mass spectrometry, kit and use method of Escherichia coli virulence gene and drug-resistant gene detection primer group

Nº publicación: CN121718645A 24/03/2026

Solicitante:

LANZHOU BAIYUAN GENE TECH CO LTD
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Resumen de: CN121718645A

The invention discloses an Escherichia coli virulence gene and drug-resistant gene detection primer group based on time-of-flight mass spectrometry, a kit and a use method of the kit. Multiple PCR amplification primers and single-base extension primers are designed according to six virulence genes escV, stx2, hlyA, rfb, uidA and eaeA of Escherichia coli and six drug-resistant genes KPC, VIM, NDM, OXA, SHV and TEM. Wherein the nucleotide sequences of the upstream amplification primer and the downstream amplification primer of each gene are respectively shown as SEQ ID NO.1-SEQ ID NO.24, and the nucleotide sequences of the single base extension primer of each gene are respectively shown as SEQ ID NO.25-SEQ ID NO.36. According to the invention, a target product is obtained through a PCR amplification reaction; carrying out a dephosphorylation reaction and an extension reaction to obtain an extension product; and carrying out desalination treatment on the obtained extension product, carrying out sample application, and analyzing by using analysis software of a mass spectrometer. The kit has the characteristics of short period, high flux, high accuracy, better flexibility, easiness in implementation, high cost performance and the like, and provides a basis for clinical early detection of Escherichia coli infection.