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SYSTEM AND METHOD FOR DETECTING, ENUMERATING, OR EXTRACTING MICROORGANISMS IN A SAMPLE

Resumen de: US20260146994A1

A system and method for detecting, enumerating, or extracting microorganisms in a sample is disclosed. Target microorganisms, such as Salmonella bacteria, may be of interest. Magnetic beads may be bound to the target microorganisms. After which, the bead-bound cells may be isolated. For example, a magnetic field may be applied in order to separate the target cells (with the magnetic beads attached thereto) and move then to a predetermined section of the well. Agar, or other immobilizing agent, may be added to the wells in order to immobilize the target cells. After which, the target cells are incubated and periodically analyzed to determine whether the target cells are growing, thereby indicating that the microorganisms are contained within the well.

DETECTION OF AN AMPHIPHILE USING VISUAL INSPECTION OF A LIGAND-MODIFIED SUBSTRATE

Resumen de: US20260133191A1

Disclosed herein are compositions, devices, systems, and methods for detection of an amphiphile using visual inspection of a ligand-modified substrate. For example, disclosed herein are assays for detection of an amphiphile via visual inspection, the assay comprising a ligand-modified substrate and a film of lubricant disposed on the ligand-modified substrate. Also disclosed herein are methods of use of any of the assays disclosed herein. In some examples, the methods comprise contacting any of the assays disclosed herein with a liquid sample; tilting the assay; and visually inspecting the liquid sample disposed on the assay to determine a property of the liquid sample. In some examples, the amphiphile comprises a biological amphiphile, such as an endotoxin. In some examples, the biological amphiphile comprises an endotoxin secreted by a gram-negative bacteria, such as Escherichia coli.

METHODS AND MEANS FOR DETECTION OF LEGIONELLA

Resumen de: EP4488391A1

The invention relates to methods for determining whether a sample comprises Legionella pneumophila, the method comprising performing an isothermal nucleic acid amplification (iNAAT) reaction, preferably loop-mediated isothermal amplification (LAMP), with said sample with a primer set specific for the Legionella pneumophila DotB or MIP gene and determining whether the sample comprises an amplification product of the amplification reaction. The invention further relates to primer sets specific for the Legionella pneumophila DotB or MIP gene that are useful in such method, and kits of part comprising such primer set.

A SYSTEM FOR RAPIDLY DETECTING AND IDENTIFYING MICROORGANISMS ON BIOLOGICAL SURFACES (E.G. FISH SKIN SURFACES) AND/OR NON-BIOLOGICAL SURFACES (E.G. HABITAT OR IC SURFACES) WITH A FLEXIBLE DISSOLVABLE CLOTH AND CORRESPONDING METHODS

Resumen de: EP4741805A1

0001 The current invention comprises systems and methods for the detection of food related bacteria, such as listeria, e. coli and s. aureus in a mobile and modular design. The combination of a dissolvable flexible cloth as sampling means, a multiple target sensor, smart readout optics and spectral processing enables to instantly ascertain, verify and validate the presence of specific microbes. The main application is the monitoring of the food and food production in order to prevent the coming to the market of bacteria invested products, such as fish, chicken or other food products destined for human consumption.

METHODS AND SYSTEMS FOR THE RAPID DETECTION OF SALMONELLA USING INFECTIOUS AGENTS

Resumen de: MX2025013800A

Disclosed herein are methods and systems for rapid detection of microorganisms in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as Salmonella-specific bacteriophage, allows detection of a specific microorganism, such as Salmonella spp. and an indicator signal may be amplified to optimize assay sensitivity.

ZIF-8 (at) AuNPs-CRISPR/Cas12a colorimetric biosensor based on assistance of smart phone and application of ZIF-8 (at) AuNPs-CRISPR/Cas12a colorimetric biosensor

Resumen de: CN121780736A

The invention discloses a ZIF-8 (at) AuNPs-CRISPR/Cas12a colorimetric biosensor based on assistance of a smart phone and application of the ZIF-8 (at) AuNPs-CRISPR/Cas12a colorimetric biosensor, a novel CRISPR/Cas12a biosensing platform is constructed based on a metal organic framework (MOF) nano enzyme (namely ZIF-8 (at) AuNPs), and the novel CRISPR/Cas12a colorimetric biosensor is used for detecting salmonella typhimurium. In the system, a CRISPR (clustered regularly interspaced short palindromic repeats) system is used as a target recognition and signal transduction module, and a ZIF-8 (at) AuNPs nano composite material with high porosity and oxidase-like activity is used as a colorimetric signal amplification module, so that the CRISPR/Cas12a-ZIF-8 (at) AuNPs colorimetric biosensor is constructed. The sensor shows an excellent linear relation to salmonella typhimurium, and the detection limit is as low as 11 CFU/mL. By establishing a CRISPR colorimetric biosensor platform assisted by a smartphone, salmonella typhimurium detection without instrument assistance is realized, and the huge potential of carrying out hyperstable field detection on food-borne pathogenic bacteria in a food supply chain is shown.

Oligonucleotide composition for female lower genital tract pathogen detection, kit and application

Resumen de: CN121780729A

The invention relates to the technical field of biological detection, and discloses an oligonucleotide composition for detecting pathogens in a female lower genital tract, a kit and application. The oligonucleotide composition disclosed by the invention can be used for rapidly and simultaneously detecting a plurality of pathogens in gardnerella vaginalis, atriurella vaginalis, prevotella vulgaris, campylobacter mobilis, bacteroides, trichomonas vaginalis and mycoplasma in a sample in a high-specificity manner, and has high sensitivity and high stability; and a rapid, non-invasive, accurate and convenient detection method is provided for female reproductive health detection.

Detection reagent for detecting bacterial cfDNA by using digital PCR, detection method and application

Resumen de: CN121780686A

The invention relates to a detection reagent and a detection method for detecting bacterial cfDNA by using digital PCR and application, a bacterial cfDNA extraction reagent and a digital PCR amplification reaction reagent are adopted as the detection reagent, primers and probe compositions for detecting the bacterial cfDNA in the digital PCR amplification reaction reagent are optimized, the primers have nucleotide sequences as shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 7 and SEQ ID NO: 8, and the probe compositions have nucleotide sequences as shown in SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 7 and SEQ ID NO: 8. The probe has nucleotide sequences as shown in SEQ ID NO: 3, SEQ ID NO: 6 and SEQ ID NO: 9, cfDNA of enterotoxin-producing bacteroides fragilis, fusobacterium nucleatum and pks + escherichia coli is subjected to qualitative detection and quantitative analysis through digital PCR, the detection method is simple, high in sensitivity, high in precision and good in stability, and the probe has important application value in detection of cfDNA of bacteria and preparation of colorectal cancer screening and early diagnosis reagents.

Double-antibody sandwich ELISA detection kit for detecting Escherichia coli BL21 LPS and application thereof

Resumen de: CN121784290A

The invention provides a double-antibody sandwich ELISA detection kit for detecting Escherichia coli BL21 LPS and application thereof, the kit comprises: an ELISA plate coated with a capture antibody, the capture antibody being a mouse polyclonal antibody against Escherichia coli BL21 LPS; the detection antibody is an anti-escherichia coli BL21 LPS (Lipopolysaccharide) rabbit polyclonal antibody; the enzyme-labeled secondary antibody is specifically combined with the detection antibody, and the enzyme-labeled secondary antibody is an HRP labeled goat anti-rabbit IgG enzyme-labeled antibody; and an Escherichia coli BL21 LPS antigen. The kit can specifically recognize LPS from Escherichia coli BL21, effectively eliminate interference of other Gram-negative bacteria LPS, realize accurate detection of target residues, provide a reliable detection means for LPS residue control of fermentation broth, intermediate products and final products in the production process of biological drugs, directly guarantee the quality safety of the biological drugs, and has a wide application prospect. The medication risk caused by LPS residues is reduced, and the health rights and interests of patients are protected.

Universal riemerella anatipestifer monoclonal antibody, detection kit and application of universal riemerella anatipestifer monoclonal antibody

Resumen de: CN121758606A

The invention relates to the technical field of poultry immunology, in particular to a universal riemerella anatipestifer monoclonal antibody, a detection kit and application of the universal riemerella anatipestifer monoclonal antibody. Specifically, the invention successfully designs the universal riemerella anatipestifer monoclonal antibody, and the detection kit developed based on the monoclonal antibody can rapidly detect the level of the riemerella anatipestifer serum antibody in a sample, and has high sensitivity and strong specificity. The riemerella anatipestifer strain has no cross reactivity with avian enterococcus faecalis, avian salmonella typhimurium, avian escherichia coli and avian salmonella enteritidis, so that the riemerella anatipestifer strain can be applied to rapid screening, epidemiological monitoring and vaccine immune effect evaluation of riemerella anatipestifer infection, and has a wide application prospect.

Method and device for detecting pathogenic bacteria

Resumen de: CN121759580A

The invention discloses a pathogenic bacterium detection method and device, and belongs to the technical field of pathogenic bacterium detection. The detection method of the pathogenic bacteria comprises the following steps: constructing a liquid drop micro-fluidic chip CRISPR/Cas reaction system: forming a water-in-oil liquid drop by a micro-fluidic chip, wherein the liquid drop contains an aptamer-block DNA double strand, a CRISPR/Cas system and a signal probe containing a labeling element; pathogenic bacteria identification and signal activation: adding a sample to be detected into the reaction system, specifically binding the pathogenic bacteria with the aptamer, and cutting the signal probe to release the labeled element; a plasma mass spectrum single-particle mode is used for detecting labeled elements, and single-bacterium level detection of pathogenic bacteria is achieved. In addition, the invention also provides a detection device for pathogenic bacteria, which comprises a micro-fluidic chip, a liquid transmission unit and a plasma mass spectrum. The detection method provided by the invention meets the low-concentration requirement of pathogenic bacteria detection, and realizes high-sensitivity pathogenic bacteria detection.

Carbapenem drug resistance gene detection primer group based on multiple PCR-time-of-flight mass spectrometry, detection kit and kit use method

Resumen de: CN121759621A

The invention discloses a carbapenem drug resistance gene detection primer group based on multiple PCR-time-of-flight mass spectrometry, a detection kit and a kit using method, and through a large number of screening and verification experiments, a multiple primer group suitable for time-of-flight mass spectrometry detection is obtained. Therefore, the multi-PCR-time-of-flight mass spectrometry detection of the multi-drug-resistant genes of carbapenem-resistant enterobacteriaceae bacteria becomes possible. The detection kit prepared by adopting the primer group can be used for simultaneously detecting and identifying 10 common carbapenem drug-resistant genes, including klebsiella pneumoniae KPC gene, NDM gene, IMP gene, VIM gene and OXA gene, acinetobacter baumannii OXA gene, NDM gene, Escherichia coli OXA gene, IMP gene and serratia marcescens VIM gene. Meanwhile, the kit is high in sensitivity and specificity, dozens of times of detection can be carried out in the same batch, so that the detection efficiency is greatly improved, and the kit is suitable for rapid screening and genetic typing of carbapenem drug-resistant bacteria in clinical samples.

Listeria monocytogenes RAA-CRISPR/Cas12a detection method based on one-tube one-step method

Resumen de: CN121759623A

The invention relates to the technical field of listeria monocytogenes detection, in particular to a listeria monocytogenes RAACRISPR/Cas12a detection method based on a one-tube one-step method. Specifically, the invention provides a novel listeria monocytogenes detection method, which is based on construction of an optimized one-tube one-step RAA-CRISPR/Cas detection system, reduces mutual interference of two reaction systems by optimizing the addition proportion of the two reaction systems, and further improves the detection sensitivity of the listeria monocytogenes by optimizing the composition of components of the RAA-CRISPR/Cas reaction systems. Comprising a buffer solution, an RAA primer, a probe, Cas12a and the like in concentration, so that the detection sensitivity and specificity are greatly improved while one-tube one-step rapid detection is realized, and a rapid and efficient listeria monocytogenes detection method with ultrahigh sensitivity and specificity is obtained.

Nucleic acid aptamer-based salmonella paratyphi A biosensor and preparation method thereof

Resumen de: CN121762836A

The invention discloses a salmonella paratyphi A biosensor based on a nucleic acid aptamer and a preparation method of the salmonella paratyphi A biosensor. Three fluorine atoms (F) are introduced into the 5'end, the 3 'end and the middle position of an aptamer 2' F3-(5 '3'm DNA) sequence (SEQ ID NO: 1) for modification, so that the binding force of the aptamer and graphene is remarkably enhanced, the signal stability and the anti-interference capability of the biosensor are remarkably improved, non-specific fluorescence leakage can be effectively blocked, and reliable guarantee is provided for complex sample detection. According to the present invention, with the cooperation of the graphene oxide quenching substrate, the high sensitivity detection of the salmonella paratyphi A is achieved, the detection limit of the sensor within 5 min can achieve 10 cells/mL, the sensitivity is high, the specificity on the complex sample is more than 93.4%, and the method is suitable for food safety rapid screening and clinical diagnosis.

Multiplex detection combination for detection of gastrointestinal bacterial nucleic acids

Resumen de: CN121773219A

Described herein are compositions, methods, and kits for detecting diarrhea-causing pathogens from a patient, food, or environmental sample. One embodiment described herein is a primer pair and probe for a multiplex polymerase chain reaction (PCR)-based assay for the detection of diarrhea-causing pathogens, such as Campylobacter spp., Salmonella spp., Shigella spp./Enteroinvasive Escherichia coli (EIEC), Escherichia coli stx1/Shiga toxin A, or Escherichia coli stx2/Shiga toxin B. Other embodiments include methods and kits for detecting diarrhea-causing pathogens.

LAMP (Loop-Mediated Isothermal Amplification) method high-throughput detection primer group and kit for five food-borne pathogenic bacteria and application thereof

Resumen de: CN121737326A

The invention discloses five food-borne pathogenic bacteria LAMP method high-throughput detection primer groups, a kit and application, and belongs to the technical field of food-borne pathogenic bacteria detection. The primer group corresponds to five bacteria, namely salmonella, vibrio parahaemolyticus, listeria monocytogenes, escherichia coli O157: H7 and staphylococcus aureus, and the nucleotide sequences of the primers are SEQ ID No.1-30. A matched system can complete the whole-process detection at the constant temperature of 62 DEG C for 45 minutes, the result does not need a complicated instrument, the positive grey and negative purple are judged by naked eyes, and the operation is simple and convenient; the detection sensitivity reaches 1-75 copies/reaction, an artificially polluted aquatic product sample does not need to be subjected to enrichment and is subjected to simple splitting decomposition and enrichment, the specificity is high, and no cross reaction exists; the anti-interference capability is outstanding, the method adapts to complex matrixes of aquatic products, and the detection coincidence rate is high; the device is simple in structure and low in cost, only needs a conventional constant temperature device, is suitable for basic food, agriculture, disease control, customs and other detection institutions, food enterprises, on-site law enforcement and on-site detection and other scenes, and provides an efficient and economic technical means for aquatic pr

Matrix type photoelectric sensing array-based pathogenic bacteria culture test detection system and pathogenic bacteria growth state judgment method

Resumen de: CN121736869A

The invention discloses a pathogenic bacteria culture test detection system based on a matrix type photoelectric sensing array and a pathogenic bacteria growth state judgment method thereof, and relates to the field of medical examination equipment. The system comprises a bearing device, a constant-temperature culture module, a swing mixing mechanism, a matrix type photoelectric sensing array and a control module, wherein each culture test tube corresponds to a plurality of transmission light detection positions on the side and a reflected light detection position at the bottom; the pathogen growth state determination method comprises the following steps: acquiring light intensity data every 10 minutes to form a continuous data curve, and analyzing and determining whether the pathogen grows or not, the surface/precipitation/turbidity growth state and the growth rate. The invention overcomes the defects of high cost, large volume, low flux and only qualitative determination in the prior art, realizes automatic, high-flux, digital and accurate detection, reduces the risk of biological pollution, and is suitable for clinical examination and quarantine institutions.

Method and device for detecting types of pathogenic bacteria in meat products

Resumen de: CN121746777A

The invention provides a meat pathogenic bacterium type detection method and device, and relates to the technical field of hyperspectral reconstruction.The method comprises the steps that on the basis of an RGB image of a to-be-detected sample of a target meat type and a pre-trained RGB-hyperspectral data reconstruction model, full-wave-band hyperspectral data of the to-be-detected sample is reconstructed; preprocessing the reconstructed full-band hyperspectral data of the to-be-detected sample; determining a characteristic wave band of the to-be-detected sample based on the preprocessed full-wave band hyperspectral data by adopting a characteristic wave band selection algorithm; extracting a characteristic spectrum corresponding to a characteristic wave band from the preprocessed full-wave band hyperspectral data; and inputting the meat type and the characteristic spectrum of the to-be-detected sample into a pre-trained pathogenic bacterium classification model, and outputting a pathogenic bacterium type classification result of the to-be-detected sample. Based on the technical scheme, the accuracy of identifying the types of pathogenic bacteria infected by meat products can be improved.

Primer group for detecting common pneumonia pathogenic bacteria of forest musk deer and application of primer group

Resumen de: CN121737324A

The invention relates to the technical field of medical detection, in particular to a primer group for detecting common pneumonia pathogenic bacteria of forest musk deer and application of the primer group. The invention provides a primer group for detecting common pneumonia pathogenic bacteria (escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa and pasteurella multocida) of forest musk deer, and successfully establishes a multiplex PCR (Polymerase Chain Reaction) method capable of simultaneously amplifying four pathogenic bacteria through a polymerase chain reaction principle. The method is high in specificity; the kit is high in sensitivity, and in a PCR reaction system of 25 microliters, the detection limit of escherichia coli is 9.2 * 10 < 4 > CFU/mL, the detection limit of klebsiella pneumoniae is 1.3 * 10 < 5 > CFU/mL, the detection limit of pseudomonas aeruginosa is 1.5 * 10 < 5 > CFU/mL, and the detection limit of pasteurella multocida is 3.7 * 10 < 4 > CFU/mL; clinical tests show that the method is good in repeatability.

Double probe for detecting salmonella as well as preparation method and application of double probe

Resumen de: CN121718608A

The invention provides a double probe for detecting salmonella as well as a preparation method and application of the double probe, and belongs to the technical field of bacterial detection. The double probes provided by the invention comprise an aptamer-phage-magnetic bead probe MB (at) Phage (at) Apt and an fDNA-gold nanoparticle probe AuNPs (at) fDNA; the sequence of the aptamer is as shown in SEQ ID NO: 1, and the sequence of the fDNA is as shown in SEQ ID NO: 2. The double probes are assisted by an ultraviolet lamp, on-site rapid visual detection of salmonella is realized through system color change caused by change of fluorescence intensity, the provided detection method is simple to operate, low in cost, less in time consumption and easy to realize, and on-site rapid visual detection of salmonella can be realized without large experimental equipment; a data analysis system and a linear equation are further utilized for calculation, the content of salmonella can be detected in a short time, and the detection efficiency and accuracy are improved.

Method for simultaneously detecting six pathogenic bacteria in cosmetics based on gene membrane chip technology

Resumen de: CN121718619A

The invention belongs to the technical field of cosmetic detection, and particularly relates to a method, a primer group and a kit for simultaneously detecting six pathogenic bacteria in cosmetics based on a gene membrane chip technology, co-enrichment culture, genome DNA extraction, multiple PCR amplification, gene membrane chip preparation and membrane chip color development detection, and an experimental result is judged according to the actual color development condition of a target. According to the present invention, the color developing points on the membrane chip are compared with the probe layout map, the internal reference color developing shows that the bacteria are detected, the color developing of each target point represents the detection of each specific bacteria, and the detection method has characteristics of simple operation, high accuracy, good repeatability, strong specificity, high stability and high sensitivity.

Escherichia coli virulence gene and drug-resistant gene detection primer group based on time-of-flight mass spectrometry, kit and use method of Escherichia coli virulence gene and drug-resistant gene detection primer group

Resumen de: CN121718645A

The invention discloses an Escherichia coli virulence gene and drug-resistant gene detection primer group based on time-of-flight mass spectrometry, a kit and a use method of the kit. Multiple PCR amplification primers and single-base extension primers are designed according to six virulence genes escV, stx2, hlyA, rfb, uidA and eaeA of Escherichia coli and six drug-resistant genes KPC, VIM, NDM, OXA, SHV and TEM. Wherein the nucleotide sequences of the upstream amplification primer and the downstream amplification primer of each gene are respectively shown as SEQ ID NO.1-SEQ ID NO.24, and the nucleotide sequences of the single base extension primer of each gene are respectively shown as SEQ ID NO.25-SEQ ID NO.36. According to the invention, a target product is obtained through a PCR amplification reaction; carrying out a dephosphorylation reaction and an extension reaction to obtain an extension product; and carrying out desalination treatment on the obtained extension product, carrying out sample application, and analyzing by using analysis software of a mass spectrometer. The kit has the characteristics of short period, high flux, high accuracy, better flexibility, easiness in implementation, high cost performance and the like, and provides a basis for clinical early detection of Escherichia coli infection.

Integrated nano platform for bimodal detection and synergistic sterilization of pathogenic bacteria as well as preparation method and application of integrated nano platform

Resumen de: CN121720929A

The invention discloses an integrated nano platform for bimodal detection and synergistic sterilization of pathogenic bacteria as well as a preparation method and application of the integrated nano platform, and belongs to the technical field of food safety. The platform is composed of a multifunctional nano probe (MPDA/Pd SA-DA-CDs/Apt) and a magnetic separation assembly (MNRs/PGA/AM). The probe integrates enzyme-like catalysis and photo-thermal performance and is used for generating colorimetric and photo-thermal dual-mode signals so as to realize high-sensitivity and high-reliability detection of pathogenic bacteria. The magnetic separation assembly is used for specifically capturing and enriching target bacteria and eliminating matrix interference. After detection is positive, the platform can immediately start secondary propulsion synergistic sterilization: firstly, the action distance is shortened through magnetic aggregation, and then pathogenic bacteria are efficiently inactivated and biological membranes are disintegrated by utilizing the synergistic effect of near-infrared laser excitation photothermal effect and active oxygen. According to the invention, the integration of detection and disinfection is realized, the operation is convenient, and the method has important application value in the fields of food safety and biomedicine.

Method for detecting residual blood through object surface disinfection wet tissue

Resumen de: CN121678650A

The invention provides a method for detecting residual blood through object surface disinfection wet tissue, and relates to the technical field of medical environment, medical equipment and equipment surface cleaning detection. After the object surface disinfection wet tissue with the residual blood detection function is used for collecting surface samples of a medical environment, a medical instrument and equipment, the residual blood pollution degree of the surfaces of the medical environment, the medical instrument and the equipment is qualitatively, semi-quantitatively and quantitatively judged according to the color change of the object surface disinfection wet tissue with the residual blood detection function. The semi-quantitative detection calibrator comprises multiple stages of color blocks and subdivision grids, and the residual blood volume is calculated by counting the area proportion of different color blocks through visual inspection; according to the quantitative detection AI rule, wet tissue images are scanned through photography and analysis equipment, software analyzes the mapping relation between pixel RGB values and standard concentration, the method is easy and convenient to operate, large-batch data can be rapidly processed, the cost is remarkably reduced, and the method is particularly suitable for rapid pollution evaluation of medical environments, medical instruments and equipment surfaces. Meanwhile, the pirameton can generate a composite reaction wi

Salmonella lateral flow chromatography biosensor

Nº publicación: CN121674407A 17/03/2026

Solicitante:

INST OF FOOD AND NUTRITION DEVELOPMENT MINISTRY OF AGRICULTURE AND RURAL AFFAIRS
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Resumen de: CN121674407A

The invention provides a salmonella lateral flow chromatography biosensor. The invention particularly relates to a sandwich lateral flow chromatography sensor consisting of a nucleic acid nano enzyme and a bacteriostatic aptamer. When a target to be detected exists, the target to be detected is combined with the nucleic acid nano-enzyme probe on the combination pad, flows through the capillary action and is specifically intercepted by the Sal5-4-17nt aptamer when passing through the T line, and the redundant nucleic acid nano-enzyme probe continuously flows to the C line through the capillary action and is intercepted and captured through hybridization among nucleic acid sequences; therefore, after the color developing solution is added, obvious blue strips appear on T and C; when the to-be-detected target does not exist, the nucleic acid nano-enzyme probe is not intercepted by the T line and is intercepted only when the to-be-detected target passes through the C line, that is, only the C line has a blue band after the developing solution is added. The test paper can realize visual detection of salmonella within 5 minutes after probe dosage and hybridization sequence design and color developing solution and color developing time optimization, and meanwhile, the test paper has good biological safety.