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OLIGOSACCHARIDE DEBRANCHING ENZYME MUTANT AND USE THEREOF IN GLUCOSE MOTHER LIQUOR

NºPublicación:  US2025122484A1 17/04/2025
Solicitante: 
JIANGNAN UNIV [CN]
JIANGNAN UNIVERSITY
US_2025122484_PA

Resumen de: US2025122484A1

The invention provides an oligosaccharide debranching enzyme mutant and use thereof in a glucose mother liquor. The mutant is obtained by mutating valine at position 219 in SEQ ID NO: 1 into alanine. According to oligosaccharide debranching enzyme mutant V219A, a primary mother liquor, a secondary mother liquor, or a tail liquid after chromatographic separation is used as a substrate, the percentage contents of glucose in the products are 99.21% (primary mother liquor), 98.89% (secondary mother liquor) and 97.97% (tail liquid after chromatographic separation) respectively, which are 2.86%, 8.64%, and 28.67% higher than that of glucose obtained with the wild-type oligosaccharide debranching enzyme. Therefore, the mutant V219A obviously improves the percentage content of glucose in the glucose mother liquor, and the scope of application of the mother liquor can be expanded by the high product purity and substrate conversion rate, so the mutant V219A has higher industrial application value.

VECTOR SYSTEM FOR TRANSFORMATION OF CHLORELLA VULGARIS, AND TRANSFORMATION METHOD FOR CHLORELLA VULGARIS

NºPublicación:  US2025122511A1 17/04/2025
Solicitante: 
GREEN MINERAL INC [KR]
GREEN MINERAL INC
US_2025122511_PA

Resumen de: US2025122511A1

The present invention relates to a vector system for transformation of Chlorella vulgaris, a Chlorella vulgaris transformation method using same, and a Chlorella vulgaris transformant.

COMPOSITIONS FOR SACCHARIFICATION OF CELLULOSIC MATERIAL

NºPublicación:  US2025122545A1 17/04/2025
Solicitante: 
NOVOZYMES INC [US]
Novozymes, Inc
US_2025122545_A1

Resumen de: US2025122545A1

The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.

Biogenic porous carbon silicon dioxide compositions and methods of making and using same

NºPublicación:  AU2025202206A1 17/04/2025
Solicitante: 
CARBON TECHNOLOGY HOLDINGS LLC
Carbon Technology Holdings, LLC
AU_2025202206_A1

Resumen de: AU2025202206A1

BIOGENIC POROUS CARBON SILICON DIOXIDE COMPOSITIONS AND METHODS OF MAKING AND USING SAME In various embodiments, the present disclosure provides biogenic porous carbon silicon dioxide compositions and methods of production and uses thereof.

PRODUCTION OF ISOPRENOIDS

NºPublicación:  US2025122551A1 17/04/2025
Solicitante: 
AMYRIS INC [US]
AMYRIS, INC
US_2025122551_PA

Resumen de: US2025122551A1

Provided herein are methods for a robust production of isoprenoids via one or more biosynthetic pathways. Also provided herein are nucleic acids, enzymes, expression vectors, and genetically modified host cells for carrying out the subject methods. Also provided herein are fermentation methods for high productivity of isoprenoids from genetically modified host cells.

SUGAR MIXTURES AND METHODS FOR PRODUCTION AND USE THEREOF

NºPublicación:  US2025122467A1 17/04/2025
Solicitante: 
INT N&H DENMARK APS [DK]
INTERNATIONAL N&H DENMARK APS
US_2025122467_A1

Resumen de: US2025122467A1

A sugar mixture comprising: monosaccharides; oligosaccharides in a ratio ≥0.06 to total saccharides; disaccharides in a ratio to total saccharides ≥0.05; pentose in a ratio to total saccharides ≥0.05; at least one alpha-bonded di-glucose; and at least one beta-bonded di-glucose. Also disclosed are methods to make and/or use such mixtures.

A MARINE FUEL BLEND

NºPublicación:  US2025122433A1 17/04/2025
Solicitante: 
NESTE OYJ [FI]
Neste Oyj
US_2025122433_PA

Resumen de: US2025122433A1

The present invention relates to a marine fuel blend having a kinematic viscosity of 2-30 mm2/s as measured at 50° C. according to EN ISO 3104:2020 and comprising 0.5-50 vol-% of palm oil effluent sludge bottom.

METHODS FOR PROVIDING PYROLYSIS OIL FROM HOLOCELLULOSE AND LIGNIN COMPRISING BIOMASS

NºPublicación:  AU2022485286A1 17/04/2025
Solicitante: 
BTG BIOLIQUIDS B V
BTG BIOLIQUIDS B.V
AU_2022485286_PA

Resumen de: AU2022485286A1

The present invention relates to methods for providing pyrolysis oil from holocellulose and lignin comprising biomass. The present methods provide, amongst others, an improved pyrolysis oil/char ratio or higher yield. The present methods additionally provide, amongst others, pyrolysis oils with improved carbon (C)/ oxygen (O) ratios or energy content. Specifically, the present invention relates to methods comprising the steps of separately introducing the biomass and a solid heat carrier with a temperature of 400°C to 700°C into a first reactor or first reaction zone; b) continuous mixing, in the absence of oxygen, of the biomass and solid heat carrier in the first reactor or the first reaction zone for providing a first time period of 0.5 to 5 seconds of pyrolytic processing yielding a mixture comprising partially pyrolyzed biomass and solid heat carrier and primary pyrolysis gas; c) introducing the mixture from the first reactor, or first reaction zone, into a second reactor, or second reaction zone, the second reactor, or second reaction zone, is configured to provide sedimentation, or separation, of solids at the bottom of the second reactor, or second reaction zone, by gravity thereby forming a layer of solids at the bottom of the second reactor, or second reaction zone; d) maintaining the mixture in the second reactor, or reaction zone, in the absence of oxygen, for a second time period of 10 to 1200 seconds of pyrolytic processing additionally yielding secondary pyro

A HARDWOOD-DERIVED CARBOHYDRATE COMPOSITION

NºPublicación:  EP4536864A1 16/04/2025
Solicitante: 
UPM KYMMENE CORP [FI]
UPM-Kymmene Corporation
KR_20250022822_A

Resumen de: FI20225522A1

A hardwood-derived carbahydrate composition comprising monomeric sugars in an amount of 65-85 weight-% based on the total dry matter content of the carbohydrate composition is disclosed. The monomeric sugars include monomeric xylose, the amount of monomeric xylose in the carbohydrate composition being 50-70 weight-% based on the total dry matter content of the carbohydrate composition. The carbonyl content of the carbohydrate composition is 5000-20000 µg/g based on the total dry matter content of the carbohydrate composition. Further is disclosed a method for producing the hardwood-derived carbohydrate composition and the use of the same.

A FERMENTATION MEDIUM COMPRISING SULPHUR

NºPublicación:  US2025115864A1 10/04/2025
Solicitante: 
EVONIK OPERATIONS GMBH [DE]
Evonik Operations GmbH
JP_2024536055_A

Resumen de: US2025115864A1

The present invention relates to an aqueous fermentation medium comprising sulphur in the form of at least one thiocarboxylate, wherein the thiocarboxylate has a chemical structure of Formula I and R═H, alkyl, COOH, COSH, and wherein the alkyl groups may also contain OH, COSH and/or COOH and wherein the concentration of the thiocarboxylate is 2 to 20 mg/L in the fermentation medium.

SORTING BIOGENIC MATERIALS ACCORDING TO A DESIRED BIOCHAR FORMULATION

NºPublicación:  WO2025075973A2 10/04/2025
Solicitante: 
AMP ROBOTICS CORP [US]
AMP ROBOTICS CORPORATION
WO_2025075973_PA

Resumen de: WO2025075973A2

Sorting biogenic material from a stream of heterogeneous materials is disclosed, including: detecting biogenic material within an input stream of heterogeneous material; sorting the biogenic material based at least in part on a desired biochar formulation; and tracking a composition of a sorted mixture of biogenic material.

WATER-RESISTANT AND DURABLE BIOCARBON PELLETS, AND METHODS FOR MAKING AND USING THE SAME

NºPublicación:  WO2025076053A2 10/04/2025
Solicitante: 
CARBON TECH HOLDINGS LLC [US]
CARBON TECHNOLOGY HOLDINGS, LLC
WO_2025076053_A2

Resumen de: WO2025076053A2

Water-resistant, high-durability, and high-density biocarbon pellets are described. In some variations, a process for producing biocarbon pellets comprises: pyrolyzing a biomass-containing feedstock in a pyrolysis reactor, thereby generating a solid biocarbon-containing material and a pyrolysis vapor; introducing the pyrolysis vapor to a separation system configured to recover a pyrolysis condensate in liquid form separated from non-condensable gases; capturing pyrolysis condensate as a polyphenolic material; contacting the solid biocarbon-containing material with the polyphenolic material, thereby generating an intermediate mixture; introducing the intermediate mixture to a densification unit, thereby generating a biocarbon pellet precursor; and at least partially drying the biocarbon pellet precursor, thereby generating biocarbon pellets characterized by a 24-hour water uptake of about 20 wt% or less, measured at 25°C and 1 bar. The biocarbon pellets can be stockpiled outside, which is logistically convenient at manufacturing sites, such as industrial metal-making plants that feed the biocarbon pellets into the process.

BIOMASS PYROLYSIS SYSTEM

NºPublicación:  WO2025073011A1 10/04/2025
Solicitante: 
BIOCARBON PTY LTD [AU]
BIOCARBON PTY LTD
WO_2025073011_PA

Resumen de: WO2025073011A1

A pyrolysis system using three different sections combined in series, of a vertical drying and gas scrubbing section, 2, a transitional section 3, and an extended horizontal high temperature reactor zone 4 based on top of a step grate furnace. The system is to efficiently convert various biomass materials into high Fixed Carbon material, clean syngas and valuable condensates by controlling feed down the vertical drying & gas scrubbing section in a packed bed-counter flow heat exchange arrangement where residence time and gas volume can be controlled through 3 feeding rate, 5 air injection, 6 & 14 recirculating gas injection. Radiant heat at 12 provides additional energy for drying the wet feed material.

WATER-RESISTANT AND DURABLE BIOCARBON PELLETS, AND METHODS FOR MAKING AND USING THE SAME

NºPublicación:  WO2025076047A1 10/04/2025
Solicitante: 
CARBON TECH HOLDINGS LLC [US]
CARBON TECHNOLOGY HOLDINGS, LLC
WO_2025076047_PA

Resumen de: WO2025076047A1

Water-resistant, high-durability, and high-density biocarbon pellets are described. In some variations, a process for producing biocarbon pellets comprises: pyrolyzing a biomass-containing feedstock in a pyrolysis reactor, thereby generating a solid biocarbon-containing material and a pyrolysis vapor; introducing the pyrolysis vapor to a separation system configured to recover a pyrolysis condensate in liquid form separated from non-condensable gases; capturing pyrolysis condensate as a polyphenolic material; contacting the solid biocarbon-containing material with the polyphenolic material, thereby generating an intermediate mixture; introducing the intermediate mixture to a densification unit, thereby generating a biocarbon pellet precursor; and at least partially drying the biocarbon pellet precursor, thereby generating biocarbon pellets characterized by a 24-hour water uptake of about 20 wt% or less, measured at 25°C and 1 bar. The biocarbon pellets can be stockpiled outside, which is logistically convenient at manufacturing sites, such as industrial metal-making plants that feed the biocarbon pellets into the process.

METHOD FOR MANUFACTURE OF A BIOMASS-BASED DIESEL FROM FEEDSTOCK FROM OLEFIN OLIGOMERS

NºPublicación:  AU2023356842A1 10/04/2025
Solicitante: 
RENEWABLE ENERGY GROUP INC
RENEWABLE ENERGY GROUP, INC
AU_2023356842_PA

Resumen de: AU2023356842A1

The present technology relates to biofuels, and more particularly, to biomass-based diesel from olefin oligomers. Diesel fuels with cetane number of 49 or greater are prepared by blending hydrocarbons produced by olefin oligomerization with renewable diesel, resulting in a blended fuel that has a lower cloud point than the cloud point of the renewable diesel. A different aspect relates to an integrated process for lipid HDO and olefin oligomerization wherein the propane coproduct of lipid HDO is subjected to dehydrogenation to produce a vapor stream having propylene and hydrogen. The propylene is subsequently oligomerized to iso-olefins and the iso-olefins are combined with the lipid feed for hydrogenation in the HDO reactor.

VARIANT POLYPEPTIDE HAVING XYLANASE ACTIVITY

NºPublicación:  US2025115888A1 10/04/2025
Solicitante: 
CJ CHEILJEDANG CORP [KR]
CJ CHEILJEDANG CORPORATION
CN_118043453_PA

Resumen de: US2025115888A1

The present disclosure relates to a modified polypeptide having xylanase activity and use thereof.

YEAST STRAINS WITH OPTIMISED WINE-MAKING PROPERTIES AND PRODUCTION METHOD THEREOF

NºPublicación:  EP4534646A1 09/04/2025
Solicitante: 
UNIV VALENCIA [ES]
CONSEJO SUPERIOR INVESTIGACION [ES]
Universitat de Val\u00E9ncia,
Consejo Superior De Investigaciones Cient\u00EDficas (CSIC)
EP_4534646_A1

Resumen de: EP4534646A1

The present invention relates to a method for producing improved strains of Saccharomyces cerevisiae with optimised wine-making properties, which comprises preparing at least one culture, wherein an initial strain of S. cerevisiae is in contact with at least 10 mg/l of 2-aminoethyl-L-cysteine in a culture medium and is grown for at least five weeks; and isolating individual mutants of the S. cerevisiae strain with optimised wine-making properties once that time has elapsed. The invention also relates to improved strains obtained using the method and to uses of same in the alcoholic fermentation of vegetable substrates.

WATER-RESISTANT AND DURABLE BIOCARBON PELLETS, AND METHODS FOR MAKING AND USING THE SAME

NºPublicación:  US2025109347A1 03/04/2025
Solicitante: 
CARBON TECH HOLDINGS LLC [US]
Carbon Technology Holdings, LLC
US_2025109347_PA

Resumen de: US2025109347A1

Water-resistant, high-durability, and high-density biocarbon pellets are described. In some variations, a process for producing biocarbon pellets comprises: pyrolyzing a biomass-containing feedstock in a pyrolysis reactor, thereby generating a solid biocarbon-containing material and a pyrolysis vapor; introducing the pyrolysis vapor to a separation system configured to recover a pyrolysis condensate in liquid form separated from non-condensable gases; capturing pyrolysis condensate as a polyphenolic material; contacting the solid biocarbon-containing material with the polyphenolic material, thereby generating an intermediate mixture; introducing the intermediate mixture to a densification unit, thereby generating a biocarbon pellet precursor; and at least partially drying the biocarbon pellet precursor, thereby generating biocarbon pellets characterized by a 24-hour water uptake of about 20 wt % or less, measured at 25° C. and 1 bar. The biocarbon pellets can be stockpiled outside, which is logistically convenient at manufacturing sites, such as industrial metal-making plants that feed the biocarbon pellets into the process.

WATER-RESISTANT AND DURABLE BIOCARBON PELLETS, AND METHODS FOR MAKING AND USING THE SAME

NºPublicación:  US2025109346A1 03/04/2025
Solicitante: 
CARBON TECH HOLDINGS LLC [US]
Carbon Technology Holdings, LLC
US_2025109346_PA

Resumen de: US2025109346A1

Water-resistant, high-durability, and high-density biocarbon pellets are described. In some variations, a process for producing biocarbon pellets comprises: pyrolyzing a biomass-containing feedstock in a pyrolysis reactor, thereby generating a solid biocarbon-containing material and a pyrolysis vapor; introducing the pyrolysis vapor to a separation system configured to recover a pyrolysis condensate in liquid form separated from non-condensable gases; capturing pyrolysis condensate as a polyphenolic material; contacting the solid biocarbon-containing material with the polyphenolic material, thereby generating an intermediate mixture; introducing the intermediate mixture to a densification unit, thereby generating a biocarbon pellet precursor; and at least partially drying the biocarbon pellet precursor, thereby generating biocarbon pellets characterized by a 24-hour water uptake of about 20 wt % or less, measured at 25° C. and 1 bar. The biocarbon pellets can be stockpiled outside, which is logistically convenient at manufacturing sites, such as industrial metal-making plants that feed the biocarbon pellets into the process.

SORTING BIOGENIC MATERIALS ACCORDING TO A DESIRED BIOCHAR FORMULATION

NºPublicación:  US2025108408A1 03/04/2025
Solicitante: 
AMP ROBOTICS CORP [US]
AMP Robotics Corporation
US_2025108408_PA

Resumen de: US2025108408A1

Sorting biogenic material from a stream of heterogeneous materials is disclosed, including: detecting biogenic material within an input stream of heterogeneous material; sorting the biogenic material based at least in part on a desired biochar formulation; and tracking a composition of a sorted mixture of biogenic material.

OBTAINING BIOGENIC MATERIAL FROM A STREAM OF HETEROGENEOUS MATERIALS

NºPublicación:  US2025108406A1 03/04/2025
Solicitante: 
AMP ROBOTICS CORP [US]
AMP Robotics Corporation
US_2025108406_PA

Resumen de: US2025108406A1

Obtaining biogenic material from a stream of heterogeneous materials is disclosed, including: receiving an input stream of heterogeneous material; separating a sub-stream of at least biogenic material from the input stream of heterogeneous material using a screen; removing a set of non-biogenic material from the sub-stream of at least biogenic material based at least in part on density separation; and drying the sub-stream of at least biogenic material after removal of the set of non-biogenic material, wherein the sub-stream of at least biogenic material after removal of the set of non-biogenic material comprises biogenic material that is suitable to produce biochar.

Expression constructs and methods of genetically engineering methylotrophic yeast

NºPublicación:  AU2025201786A1 03/04/2025
Solicitante: 
IMPOSSIBLE FOODS INC
Impossible Foods Inc
AU_2025201786_A1

Resumen de: AU2025201786A1

Methods and materials for genetically engineering methylotrophic yeast are provided, such as a methylotrophic yeast cell comprising a recombinant nucleic acid molecule, wherein the 5 recombinant nucleic acid molecule comprises an exogenous nucleic acid encoding a transcriptional activator operably linked to at least one methanol-inducible promoter element.

EFFICIENT REMOVAL AND STORAGE OF CARBON DIOXIDE

NºPublicación:  AU2023342163A1 03/04/2025
Solicitante: 
CARBA INC
CARBA INC
AU_2023342163_PA

Resumen de: AU2023342163A1

A process is hereby provided for permanently (>1000 years) reducing carbon dioxide in the atmosphere. The process involves the cultivation of tree and plant waste (biomass), the conversion of the biomass in a reactor to a carbon product, e.g., charcoal, and subsequent storage of the carbon product. The reactor used for conversion is run at a low temperature, e.g., 300-450°C. Thus, there is negligible cracking. The product is primarily a solid carbon product, which is safe and can be easily handled. The carbon product can also be safely stored. In one embodiment, the carbon product is buried in a location that is tested for limited oxygen at burial depths. The permanence of the CO; reduction is therefore assured.

METHOD FOR CHARACTERIZING INTERACTION FORCE BETWEEN LIGNIN AND CELLULASE

NºPublicación:  US2025110152A1 03/04/2025
Solicitante: 
NANJING FORESTRY UNIV [CN]
NANJING FORESTRY UNIVERSITY
US_2025110152_PA

Resumen de: US2025110152A1

A method for characterizing the interaction force between lignin and cellulase is provided. The method includes the following steps: (1) preparation of lignin film: preparing a lignin solution, then dropwise adding the solution onto a silicon wafer, repeatedly spin-coating with a spin coater, and vacuum-drying the prepared lignin film; (2) AFM probe modification: modifying an AFM probe coated with a gold film on the surface with HS-PEG-COOH and then activating the carboxyl group, immersing the AFM probe after the carboxyl group activation in a PBS solution of cellulase for incubation, and after the modification is completed, placing the washed AFM probe in a PBS buffer solution for storage for later use; (3) testing the lignin-cellulase interaction force by using an AFM instrument in a liquid phase environment. AFM is used to quantitatively characterize the interaction between the cellulase and lignin in a liquid environment.

METHOD FOR BLENDED FUEL COMPOSITIONS WITH IMPROVED EMISSIONS PROFILES

Nº publicación: EP4530333A2 02/04/2025

Solicitante:

REG SYNTHETIC FUELS LLC [US]
REG Synthetic Fuels, LLC

EP_4530333_PA

Resumen de: EP4530333A2

The present technology provides blended fuel compositions and methods of generating such compositions that exhibit surprising and unexpected emissions profiles, while avoiding field performance issues, where the blended fuel composition includes about 5 vol.% to about 95 vol.% of synthetic paraffinic diesel; about 5 vol.% to about 95 vol.% of a biodiesel; and about 0 vol.% to about 90 vol.% of a petroleum diesel, provided that at least about 8 vol.% biodiesel is included when the blended fuel composition does not include petroleum diesel.

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