Resumen de: US2025277157A1
This invention provides processes and systems for converting biomass into high-carbon biogenic reagents that are suitable for a variety of commercial applications. Some embodiments employ pyrolysis in the presence of an inert gas to generate hot pyrolyzed solids, condensable vapors, and non-condensable gases, followed by separation of vapors and gases, and cooling of the hot pyrolyzed solids in the presence of the inert gas. Additives may be introduced during processing or combined with the reagent, or both. The biogenic reagent may include at least 70 wt %, 80 wt %, 90 wt %, 95 wt %, or more total carbon on a dry basis. The biogenic reagent may have an energy content of at least 12,000 Btu/lb, 13,000 Btu/lb, 14,000 Btu/lb, or 14,500 Btu/lb on a dry basis. The biogenic reagent may be formed into fine powders, or structural objects. The structural objects may have a structure and/or strength that derive from the feedstock, heat rate, and additives.
Resumen de: US2025277205A1
A recombinant yeast cell functionally expressing:a) a heterologous nucleic acid sequence encoding a protein having ribulose-1,5-phosphate carboxylase/oxygenase activity (EC4.1.1.39; Rubisco), a heterologous nucleic acid sequence encoding a protein having phosphoribulokinase activity (EC2.7.1.19; PRK) and optionally one or more nucleic acid sequences encoding for molecular chaperones for the protein having ribulose-1,5-phosphate carboxylase/oxygenase activity; andb) a nucleic acid sequence encoding a protein having transketolase activity (EC 2.2.1.1), wherein the expression of the nucleic acid sequence encoding the protein having transketolase activity is under control of a promoter (the “TKL promoter”), which TKL promoter has an anaerobic/aerobic expression ratio for the transketolase of 2 or more.
Resumen de: US2025277240A1
A recombinant yeast cell functionally expressing: —a nucleic acid sequence encoding a native protein having transketolase activity (EC 2.2.1.1); and —a nucleic acid sequence encoding a heterologous protein having transketolase activity (EC 2.2.1.1).
Resumen de: WO2024091515A1
A method for quantifying a cellulosic component in a sample includes preparing a first suspension by mixing an amount of sample with first aqueous acid at a concentration of 40 wt-% or greater; incubating the first suspension in a first incubation stage at a first incubation temperature of less than 40 °C; adding water or aqueous sulfuric acid to the incubated first suspension to form a second suspension having a concentration 40 wt-% or less of sulfuric acid based on a total weight of the second suspension; incubating the second suspension in a second incubation stage at a second incubation temperature of 50 °C or greater for an incubation period, wherein the sample is not subjected to hydrolysis at temperatures above 98 °C; separating a supernatant from the incubated second suspension; analyzing the supernatant for total content of xylose, mannose, galactose, and arabinose; and calculating, based on the analysis, the amount of the hemicellulose.
Resumen de: EP4610335A1
The present invention relates to a process for producing a FAGE-based biofuel composition, the process being characterized by an enzymatic transesterification of fatty acid alkyl esters with glycerol formal. The invention is also directed to the biofuel composition obtained by said process as well as to the uses thereof.
Resumen de: US2025270597A1
Disclosed herein are genetically engineered yeast cells capable of producing lactate from sucrose. The genetically engineered yeast cells comprise a polynucleotide encoding an exogenous lactate dehydrogenase enzyme; a polynucleotide encoding an exogenous invertase enzyme; a deletion or disruption of a native pyruvate decarboxylase (PDC) gene; and a genetic modification resulting in overexpression of a native hexokinase gene.
Resumen de: US2025270595A1
Described is a method for the production of isobutene from a carbon source characterized in that it comprises: (a) culturing a microorganism capable of producing 3-methylcrotonic acid from a carbon source in a liquid culture medium, thereby producing said 3-methylcrotonic acid so that it accumulates in the liquid culture medium; and (b) enzymatically converting said 3-methylcrotonic acid contained in the liquid culture medium obtained in step (a) into isobutene by: (i) incubating a microorganism expressing an FMN-dependent decarboxylase associated with an FMN prenyl transferase with said liquid culture medium containing 3-methylcrotonic acid obtained in step (a); and/or (ii) incubating an FMN-dependent decarboxylase associated with an FMN prenyl transferase with said liquid culture medium containing 3-methylcrotonic acid obtained in step (a); thereby producing said isobutene; and (c) recovering the produced isobutene.
Resumen de: US2025270496A1
The present invention relates to a method for the production of a fungal fermentation medium from at least one lignocellulosic material, preferably at least one industrial and/or agricultural side stream and to a fermentation medium obtainable accordingly, to a method for production of a fungal biomass by submerged fermentation of at least one fungal strain and to a fungal biomass obtainable accordingly, and to a fungal-based food product obtainable by using the instant fungal biomass of the invention. The instant fermentation medium produced preferably from spent grain is particularly useful in production of fungal biomass by submerged fermentation of Pleurotus pulmonarius, among others.
Resumen de: US2025270606A1
Systems and methods for producing carbohydrate (e.g., sugar) streams (and recycling enzymes) from a pretreated or untreated biomass such as cellulosic feedstock, including, for example, “brown stock” feedstock, or waste or recycled fiber sludge produced in the pulp and paper industry, such as for biochemical (e.g., biofuel) production, are provided. In one example, the system and method can produce high purity C6 (glucose and/or fructose) and/or C5 (xylose) sugar streams, and other carbohydrates and/or fibrous materials, from cellulosic feedstocks, such as brown stock or waste fiber sludge, that can be effectively converted into various biochemical products, such as ethanol.
Resumen de: US2025269296A1
This disclosure describes energy efficient process to distill a process stream in a production facility. A process uses multiple effect evaporators, ranging from one evaporator to eight evaporators in each effect. The process arrangement shows an example of four effect evaporators, with a zero-effect evaporator having a single evaporator, a first-effect evaporator having a set of three evaporators, a second-effect evaporator having a set of three evaporators, and a third-effect evaporator having a set of evaporators to create condensed distillers solubles.
Resumen de: EP4606809A2
Provided are compositions and processes concerning efficient downstream processing of products derived from organic acids pretreatment of plant materials.
Resumen de: US2025263756A1
The present disclosure concerns a symbiotic combination of host cells engineered to produce a first metabolic product, for example a carbohydrate, and to convert the second metabolic product into a second metabolic product, for example an alcohol.
Resumen de: US2025263348A1
A method is provided for producing a biochar solution. The method comprises the steps of collecting biochar particles, dispersing the biochar particles in a liquid solution and adding a stabilizing agent to keep the biochar in flowable suspension. The stabilizing agent may be added to the liquid solution or to the biochar prior to placing the biochar in solution.
Resumen de: US2025262647A1
Sorting biogenic material from a stream of heterogeneous materials is disclosed, including: detecting biogenic material within an input stream of heterogeneous material; sorting the biogenic material based at least in part on a desired biochar formulation; and tracking a composition of a sorted mixture of biogenic material.
Resumen de: US2025263755A1
The present invention relates to a process for enzymatic (trans)esterification/esterification of free fatty acids and glycerides. In particular, the invention relates to this process using a drying operation for water removal from enzyme reaction mixture continuously or by separating the glycerol phase from the reaction mixture, then drying the glycerol phase and recirculating to reform the reaction mixture, especially in biodiesel applications, which facilitates reduction of FFA in the biodiesel.
Resumen de: US2025263652A1
A method for contamination control when growing yeasts is provided. Bacterial contamination is controlled by using urea as the primary nitrogen source while simultaneously limiting the amount of nickel available to contaminating bacteria. Bacteria require nickel as a cofactor for urease enzymes in order to use urea for growth while yeasts do not require nickel as a cofactor for any enzymes. Nickel is limited by using titanium in plate heat exchangers instead of stainless steel. Ethyl carbamate is limited by using a carbon/nitrogen ratio that consumes all urea during fermentation and by separating co-products after fermentation and before distillation. Yeast recycling is performed by using either single-step or two-step centrifugation, without acid washing. This method enables yeast recycling with sugarcane ethanol and sugar beet ethanol production. This method also enables yeast recycling with corn ethanol and grain ethanol production with coproduct recovery after fermentation and before distillation.
Resumen de: EP4603575A1
Provided is Hyphomicrobium denitrificans MQ004-2, which was deposited at the China Center for Type Culture Collection on July 8, 2022, with an accession number of CCTCC NO: M20221066. The problems that a PQQ fermentation yield is not high, and the metabolic process of Hyphomicrobium denitrificans is unstable, which consequently makes it difficult to achieve large-scale production are solved. The strain has a high titer level, a stable titer level between batches, a small methanol consumption difference, and good strain metabolism stability and facilitates large-scale production.
Resumen de: CN120019186A
A conveyor (1) for pretreated biomass (4) wherein the conveyor (1) comprises: a cylindrical housing (2) comprising an inlet (5) for receiving pretreated biomass (4) and an outlet (6) from which pretreated biomass (4) is discharged from the cylindrical housing (2); and at least one shaftless screw (3, 3b) rotatably positioned in the cylindrical housing (2) to remove the pretreated biomass (4) from the cylindrical housing (2). The temperature in the cylindrical shell (2) ranges from 120 DEG C to 250 DEG C. Systems, uses, and methods are also disclosed.
Resumen de: US2025257277A1
Processes and systems for converting biomass into high-carbon biogenic reagents that are suitable for a variety of commercial applications. Pyrolysis in the presence of an inert gas is employed to generate hot pyrolyzed solids, condensable vapors, and non-condensable gases, followed by separation of vapors and gases, and cooling of the hot pyrolyzed solids in the presence of the inert gas. Additives may be introduced during processing or combined with the reagent, or both. The biogenic reagent may include at least 70 wt %, 80 wt %, 90 wt %, 95 wt %, or more total carbon on a dry basis. The biogenic reagent may have an energy content of at least 12,000 Btu/lb, 13,000 Btu/lb, 14,000 Btu/lb, or 14,500 Btu/lb on a dry basis. The biogenic reagent may be formed into fine powders, or structural objects. The structural objects may have a structure and/or strength that derive from the feedstock, heat rate, and additives.
Resumen de: US2025257527A1
Processes disclosed are capable of converting biomass into high-crystallinity nanocellulose with low mechanical energy input. In some variations, the process includes fractionating biomass with sulfur dioxide or a sulfite compound and water, to generate cellulose-rich solids and a liquid containing hemicellulose and lignin; and mechanically treating the cellulose-rich solids to form nanofibrils and/or nanocrystals. The total mechanical energy may be less than 500 kilowatt-hours per ton. The crystallinity of the nanocellulose material may be 80% or higher, translating into good reinforcing properties for composites. The nanocellulose material may include nanofibrillated cellulose, nanocrystalline cellulose, or both. In some embodiments, the nanocellulose material is hydrophobic via deposition of some lignin onto the cellulose surface. Optionally, sugars derived from amorphous cellulose and hemicellulose may be separately fermented, such as to monomers for various polymers. These polymers may be combined with the nanocellulose to form completely renewable composites.
Resumen de: US2025256260A1
Highly mesoporous activated carbon products are disclosed with mesoporosities characterized by mesopore volumes of 0.7 to 1.0 cubic centimeters per gram or greater. Also disclosed are activated carbon products characterized by a Molasses Number of about 500 to 1000 or greater. Also disclosed are activated carbon products characterized by a Tannin Value of about 100 to 35 or less. The activated carbon products may be further characterized by total pore volumes of at least 0.85 cubic centimeters per gram and BET surface areas of at least about 800 square meters per gram. The activated carbon product may be derived from a renewable feedstock.
Resumen de: US2025257375A1
The present invention provides a process for producing fatty acid alkyl esters. The process comprises providing a substrate comprising triglycerides, diglycerides, monoglycerides, free fatty acids, or any combination thereof, and reacting the substrate with an enzyme composition comprising an sn-1,3 position lipase and an sn-2 position lipase to produce fatty acid alkyl esters.
Resumen de: US2025257278A1
Processes and systems for converting biomass into high-carbon biogenic reagents that are suitable for a variety of commercial applications. Pyrolysis in the presence of an inert gas is employed to generate hot pyrolyzed solids, condensable vapors, and non-condensable gases, followed by separation of vapors and gases, and cooling of the hot pyrolyzed solids in the presence of the inert gas. Additives may be introduced during processing or combined with the reagent, or both. The biogenic reagent may include at least 70 wt %, 80 wt %, 90 wt %, 95 wt %, or more total carbon on a dry basis. The biogenic reagent may have an energy content of at least 12,000 Btu/lb, 13,000 Btu/lb, 14,000 Btu/lb, or 14,500 Btu/lb on a dry basis. The biogenic reagent may be formed into fine powders, or structural objects. The structural objects may have a structure and/or strength that derive from the feedstock, heat rate, and additives.
Resumen de: US2025257371A1
The present invention provides a method for making ethanol and a protein feed or food product from a feedstock including starch, such as grain and grain-derived products, preferably combined with CO2 capture to increase yields further. The method facilitates a reduction in water usage compared to traditional ethanol plants, without affecting quality and quantity of the end products.
Nº publicación: US2025257374A1 14/08/2025
Solicitante:
KIVERDI INC [US]
Kiverdi, Inc
Resumen de: US2025257374A1
The invention described herein presents compositions and methods for a multistep biological and chemical process for the capture and conversion of carbon dioxide and/or other forms of inorganic carbon into organic chemicals including biofuels or other useful industrial, chemical, pharmaceutical, or biomass products. One or more process steps utilizes chemoautotrophic microorganisms to fix inorganic carbon into organic compounds through chemosynthesis. An additional feature described are process steps whereby electron donors used for the chemosynthetic fixation of carbon are generated by chemical or electrochemical means, or are produced from inorganic or waste sources. An additional feature described are process steps for recovery of useful chemicals produced by the carbon dioxide capture and conversion process, both from chemosynthetic reaction steps, as well as from non-biological reaction steps.