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Detection method of psychrophilic bacteria, primer pair, probe and application

Resumen de: CN121006407A

The invention belongs to the technical field of bacterium detection, and particularly relates to a psychrophilic bacterium detection method, a primer pair, a probe and application. The detection method comprises the following step: carrying out amplification detection by using one or more primer pairs and/or probes in a TY1F and TY1R primer pair and/or probe, a TY2F and TY2R primer pair and/or probe and a TY3F and TY3R primer pair and/or probe. According to the detection method, the psychrophilic bacteria in the sample can be rapidly detected, the species proportion of gram-negative bacteria, pseudomonas and pseudomonas fluorescens which is high in harm and produces alkaline metalloenzyme in the psychrophilic bacteria can be clearly determined, and the condition of the psychrophilic bacteria in the sample (such as raw milk) can be better controlled.

Intracellular pH value detection method in process of expressing heterologous protein by escherichia coli and application of intracellular pH value detection method

Resumen de: CN121007876A

The invention belongs to the technical field of single cell detection, and particularly relates to a method for detecting the intracellular pH value in the process of expressing heterologous protein by escherichia coli and application. The detection method comprises the following steps: S1, establishing a pH response curve; s2, co-incubating the fluorescent probe AF-C and the escherichia coli in the process of expressing the heterologous protein to obtain a real-time intracellular cell image of the escherichia coli; s3, processing the real-time cell image in the step S2, and calculating the fluorescence intensity of a Red channel and the fluorescence intensity of an NIR channel corresponding to a single cell; and substituting the ratio of the fluorescence intensity of the Red channel to the fluorescence intensity of the NIR channel into the pH response curve in the step S1 to obtain the intracellular pH value of the escherichia coli. The detection method provided by the invention has good permeability and retention in the process of expressing heterologous protein by escherichia coli, has fast spectral response to intracellular pH change, and is more accurate in detection result.

Method for eliminating interference introduced by host cells in detection kit and kit

Resumen de: CN121008042A

The invention provides a method for eliminating interference introduced by host cells in a detection kit and the kit. The method for eliminating interference introduced by the host cells in the detection kit comprises the step of adding an anti-interference agent into the kit, the anti-interference agent is host cell endogenous protein, the molecular weight is 20-60 kD, and the final concentration of the anti-interference agent is 40 mu g/mL or above. The method can effectively eliminate the influence of Escherichia coli endogenous protein in the detection kit on the detection result.

Detection method of psychrophilic bacteria, primer pair and application

Resumen de: CN121006406A

The invention belongs to the technical field of bacterium detection, and particularly relates to a psychrophilic bacterium detection method, a primer pair and application. The detection method comprises the following step: carrying out amplification detection by using one or more of a TY1F primer pair and a TY1R primer pair, a TY2F primer pair and a TY2R primer pair, and a TY3F primer pair and a TY3R primer pair. According to the detection method, the psychrophilic bacteria in the sample can be rapidly detected, the species proportion of gram-negative bacteria, pseudomonas and pseudomonas fluorescens which is high in harm and produces alkaline metalloenzyme in the psychrophilic bacteria can be clearly determined, and the condition of the psychrophilic bacteria in the sample (such as raw milk) can be better controlled.

Kit and detection method for detecting pathogens in sample

Resumen de: CN120989266A

The invention provides a primer group for specifically detecting pathogens in a sample. The primer group comprises at least one of a first primer group for specifically targeting a malB gene of escherichia coli, a second primer group for specifically targeting an invA gene of salmonella and a third primer group for specifically targeting an ipaH gene of shigella. The invention also provides a kit and a detection method for detecting pathogens in a sample by adopting the primer group.

Method for detecting five pilus adhesion subtypes of enterotoxigenic escherichia coli in pig farm based on 5-plex mPCR

Resumen de: CN120989270A

The invention provides a method for detecting five pilus adhesion subtypes of enterotoxigenic escherichia coli in a pig farm based on 5-plex mPCR, and belongs to the technical field of biological detection. The multiplex PCR primer group comprises a primer pair for detecting a fimbriae gene of escherichia coli F5, a primer pair for detecting a fimbriae gene of escherichia coli F18, a primer pair for detecting a fimbriae gene of escherichia coli F6, a primer pair for detecting a fimbriae gene of escherichia coli F4 and a primer pair for detecting a fimbriae gene of escherichia coli F41. The invention provides a method for detecting five fimbristin subtypes of enterotoxigenic escherichia coli in a pig farm based on 5-plex mPCR, which constructs a stable mPCR system capable of completing synchronous detection of five serum subtypes (F4, F18, F6, F41 and K99) at one time, and realizes synchronous detection of fimbristin genotypes.

Rapid detection method for food-borne pathogenic bacteria based on interference of meat-derived symbiotic microorganisms

Resumen de: CN120989206A

The invention relates to a rapid detection method for food-borne pathogenic bacteria based on the action of meat-derived symbiotic microorganisms, belongs to the technical field of rapid detection and spectral analysis of fresh meat quality, and aims to solve the problems of tedious detection operation and high dependency on biochemical reagents caused by interference of a complex substrate and symbiotic microorganisms of fresh meat in the prior art. The method comprises the following steps: firstly, carrying out elution separation-homogeneous centrifugation-coating culture pretreatment on a fresh meat sample to eliminate the interference of a fresh meat matrix, and then preferably selecting a high-discrimination characteristic wave band from high-dimensional spectral data by applying a combined characteristic screening algorithm; and finally, detecting the food-borne pathogenic bacteria of the fresh meat by utilizing a qualitative and quantitative model established on the basis of a culture sample under the interference of the meat-borne symbiotic microorganisms in the earlier stage. The detection accuracy and sensitivity of the food-borne pathogenic bacteria in the fresh meat can be improved, meanwhile, the method has the advantages of being rapid, easy and convenient to operate and economical in cost, and technical support can be provided for detection of the food-borne pathogenic bacteria in the fresh meat.

Method for detecting pseudomonas aeruginosa in food and application thereof

Resumen de: CN120992570A

The invention discloses a method for detecting pseudomonas aeruginosa in a beverage, which comprises the following steps: step 1, mixing and incubating a pseudomonas aeruginosa aptamer solution and a colloidal gold solution, then adding a NaCl solution, uniformly mixing and reacting to obtain a mixed solution; step 2, mixing and incubating the mixed solution and a to-be-detected sample solution, then adding a Tb-MOFs solution, and uniformly mixing and reacting to obtain a to-be-detected precursor solution; and 3, measuring a fluorescence signal of the precursor solution to be measured. According to the method, detection can be realized by using a fluorescence spectrometer, the detection range is 1-106 CFU/mL, and the detection limit is 0.63 CFU/mL. The terbium-based metal organic framework material is used as a fluorescence signal element for the first time, and the aptamer is used as a target recognition element, so that the fluorescent probe has good anti-interference capability and is suitable for solution systems such as water and beverages. In addition, the method does not need strain culture and has the advantages of simplicity and convenience in operation, high sensitivity and the like.

Endogenous protein activated deoxyribozyme-based pathogen viable bacteria detection kit

Nº publicación: CN120989218A 21/11/2025

Solicitante:

UNIV SICHUAN
\u56DB\u5DDD\u5927\u5B66

Resumen de: CN120989218A

The invention provides a pathogen viable bacteria detection kit based on endogenous protein activated deoxyribozyme. The kit comprises a deoxyribozyme recognition element and a signal transduction element. The deoxyribozyme recognition element is composed of a substrate chain and an enzyme chain, and the signal transduction element is composed of two DNA reporter probes (Sub-F and Sub-Q), wherein the tail ends of the two DNA reporter probes are respectively marked with a fluorescence staining group and a fluorescence quenching group. The deoxyribozyme recognition element can recognize specific endogenous protein of target pathogenic viable bacteria and activate an enzyme chain to cut a substrate chain to generate two independent substrate chain fragments. And then a signal transduction element is introduced to realize specific output of a fluorescence signal. The kit provided by the invention can realize detection of living salmonella by targeting endogenous protein, can sensitively detect 190 CFU/mL of living salmonella with abundance as low as 0.1%, and has a good application prospect in the directions of food safety detection and clinical examination.