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Fluorescent lateral flow chromatography detection method of salmonella antibody based on perovskite quantum dots and magnetic nanoparticles

Resumen de: CN120102874A

The invention belongs to the field of salmonella detection, and discloses a fluorescent lateral flow chromatography detection method for detecting salmonella, which realizes sensitive detection of salmonella through combination of CsPbBr3 (at) SiO2 with excellent fluorescence performance and Fe3O4 with magnetic response capability. The problems that in the prior art, real-time on-site antibody detection is long in time consumption, labor-consuming, high in cost, unable to quantify and the like are solved. The fluorescent probe IPNs capable of specifically recognizing salmonella is obtained by coating the outside of perovskite quantum dots with silicon dioxide, and meanwhile, the surfaces of Fe3O4 nanoparticles are modified with salmonella antibodies to prepare the magnetic probe IMNs. The former provides a fluorescence signal, and the latter realizes rapid separation of bacteria, so that the detection is more sensitive, rapid and convenient, and is suitable for various scenes. Human eyes are highly sensitive to green fluorescence, preliminary qualitative detection can be realized without a fluorescence detector, the method is suitable for field detection, and the method is excellent in specificity, anti-interference capability and repeatability and has potential in actual detection.

PREPARATION OF LIVE VACCINES

Resumen de: US2025177508A1

Described is a method for the generation of a live vaccine containing stable bacteria carrying at least three attenuating mutations and a vaccine containing bacteria obtained by said method.

THERMOSTABLE UV INACTIVATED VACCINES AND OTHER BIOPHARMACEUTICALS

Resumen de: US2025177534A1

This invention describes method for inactivation of microorganisms in thermostable dry formulations at ambient temperatures (AT) using UV light irradiation. According to this method microorganisms are inactivated at ambient temperatures (AT) in dry formulations where the amount of free radicals formed is relatively small and damage of nucleic acids is the main cause for the microorganism's death. The method will allow production of thermostable inactivated vaccines from wild type and live attenuated microorganisms, thermostable inactivated microbiome products, and thermostable sterilized none-live blood components, therapeutic proteins, antibodies and other fragile biopharmaceuticals.

DEVELOPMENT OF CARBOHYDRATE-BASED ANTI-SALMONELLA VACCINES

Resumen de: AU2023312737A1

Provided herein are vaccine composition comprising a

Bacillus coagulans and application thereof

Resumen de: CN120082456A

The invention provides bacillus coagulans and application thereof, relates to the technical field of microorganisms, and aims to solve the problem that a biological material with a strong antibacterial function and good tolerance is lacked in the prior art. The invention provides bacillus coagulans XH-2, the preservation number of the bacillus coagulans XH-2 is CCTCC NO: M 2023759, the bacillus coagulans XH-2 is preserved in the China Center for Type Culture Collection on May 15, 2023, and the preservation address is Wuhan University, China. The bacillus coagulans XH-2 disclosed by the invention has a relatively strong inhibition effect on escherichia coli, and also has a relatively good inhibition effect on salmonella gallinarum, salmonella suis and salmonella typhimurium at the same time. According to the invention, the bacillus coagulans XH-2 is used as an active ingredient to prepare the livestock or poultry feed additive, and the strain has strong gastric juice and cholate tolerance, and can inhibit the growth of pathogenic bacteria and improve the conversion and utilization of animal feed.

一种鼠伤寒沙门菌特异性抗原片段及其在检测鼠伤寒沙门菌抗体中的应用

Resumen de: CN120081916A

本发明公开了一种鼠伤寒沙门菌特异性抗原片段及其在检测鼠伤寒沙门菌抗体中的应用。所述鼠伤寒沙门菌特异性抗原片段的氨基酸序列如SEQ ID NO.1所示。本发明首次公开了以鼠伤寒沙门菌特异性抗原片段作为间接ELISA的包被抗原，建立了鼠伤寒沙门菌间接ELISA抗体检测方法，鼠伤寒沙门菌特异性抗原片段含有鼠伤寒沙门菌FliC蛋白的优势B细胞表位，能够特异性识别诱导产生的鼠伤寒沙门菌FliC蛋白抗体，在提高抗原抗体特异性结合方面有显著性的增效作用。本发明能够用于鼠伤寒沙门菌抗体的检测，为鼠伤寒沙门菌的流行病学调查以及沙门菌的防控提供了有力的技术支持。

Multi-epitope fusion antigen of multiple toxins and fimbriae of porcine diarrhea source escherichia coli and application of multi-epitope fusion antigen

Resumen de: CN120081954A

The invention provides a multi-epitope fusion antigen of multiple toxins and fimbriae of porcine diarrhea source escherichia coli and application of the multi-epitope fusion antigen. The amino acid sequence of the multi-epitope fusion antigen is shown as SEQ ID NO. 1. The multi-epitope fusion antigen MEAET prepared by the invention can be used for preparing an antibody and can also be used for preparing a product for detecting a pathogenic escherichia coli antibody. According to the invention, epitopes of multiple toxin/pilus antigens of porcine diarrhea source escherichia coli are analyzed, and the multiple antigen epitopes are optimized and arranged to obtain MEAET which can simultaneously recognize antibodies of multiple antigens. Indirect ELISA specificity tests show that the MEAET does not have cross reaction with positive serum of other common pathogens, only has good reaction with positive serum of porcine diarrhea escherichia coli, has small cross reaction with a symbiotic escherichia coli antibody, is suitable for detecting the condition of the porcine diarrhea escherichia coli antibody, and is convenient for large-scale sample detection and epidemiological monitoring.

Detection primer probe combination for six food-borne pathogens and application thereof

Resumen de: CN120082663A

The invention relates to the technical field of microbiological detection, and particularly discloses a primer probe combination for detecting six food-borne pathogens and application of the primer probe combination. The detection primer probe combination for the six food-borne pathogens comprises six pairs of specific primer pairs and six probes, the nucleotide sequences of the specific primer pairs are respectively shown as SEQ ID NO.1, SEQ ID NO.4, SEQ ID NO.8, SEQ ID NO.1, SEQ ID NO.13, SEQ ID NO.16, SEQ ID NO.19, SEQ ID NO.22, SEQ ID NO.26, SEQ ID NO.29, SEQ ID NO.32 and SEQ ID NO.35, and the nucleotide sequences of the probes are respectively shown as SEQ ID NO.37-SEQ ID NO.42; the six food-borne pathogens comprise listeria monocytogenes, salmonella, vibrio parahaemolyticus, norovirus, escherichia coli O157: H7 and staphylococcus aureus. According to the present invention, the pathogens of six common food-borne diseases can be detected, the detection method has characteristics of high specificity and high sensitivity, and the pathogens do not interfere with each other and do not inhibit each other during the detection so as to achieve good stability.

Enterococcus faecium HKS023 and application thereof

Resumen de: CN120060052A

The invention discloses a strain of enterococcus faecium HKS023 and application of the enterococcus faecium HKS023. The enterococcus faecium HKS023 is preserved in China General Microbiological Culture Collection Center on February 14, 2023, and the preservation number of the enterococcus faecium HKS023 is CGMCC No.26552. The enterococcus faecium HKS023 is named as enterococcus faecium HKS023. According to the invention, a new strain of enterococcus faecium HKS023 is screened from fresh feces of healthy weaned piglets, corn flour is taken as a raw material, L-lactic acid is produced through fermentation, the acid yield is high, and the enterococcus faecium HKS023 has a very strong inhibition effect on the growth of salmonella and can inhibit the growth of escherichia coli and staphylococcus aureus to a certain extent. The enterococcus faecium HKS023 microbial feed additive is produced by effectively combining and fermenting common raw materials including calcium carbonate, beef extract, yeast extract, peptone, glucose, sodium acetate, dipotassium phosphate, magnesium sulfate, manganese sulfate and the like, the growth performance of livestock and poultry can be effectively improved, the diarrhea rate is reduced, the immunity of the livestock and poultry is improved, and the economic and social benefits are huge.

Medical application of apigenin in preparation of salmonella flagellum inhibitor

Resumen de: CN120053424A

The invention belongs to the technical field of anti-infection drugs, and particularly relates to medical application of apigenin in preparation of a salmonella flagellum inhibitor. A migration inhibition test (0.3% agar), minimum inhibitory concentration (MIC) determination, a Caco-2 cell adhesion test and a molecular docking test prove that the apigenin can specifically inhibit salmonella flagellum-mediated migration ability (inhibition rate gt; 49%) and host cell adhesion capability (reduced by more than or equal to 58%), and does not affect bacterial growth (pgt; 0.05), the apigenin can be used for targeting flagellin FljB, FliC, FlgN, FlgA and FlgG (binding energy per lt; -5kcal/mol) can block flagellar movement, and has an application prospect in prevention and control of livestock salmonella related diseases.

Preparation method of surface cleaning disinfectant for fresh fruits and vegetables and application of surface cleaning disinfectant in fruit and vegetable cleaning

Resumen de: CN120052369A

The invention discloses a preparation method of a fresh fruit and vegetable surface cleaning disinfectant and application of the fresh fruit and vegetable surface cleaning disinfectant in fruit and vegetable cleaning.The preparation method comprises the steps that firstly, the antibacterial effect of citric acid, tartaric acid and sodium hypochlorite is evaluated, the synergistic antibacterial effect of different compounding modes is optimized, and meanwhile the optimal compounding formula is screened out; and the antibacterial activity is verified by using a time-sterilization curve. The compound disinfectant has an obvious inhibition effect on typical food-borne pathogenic bacteria such as salmonella typhimurium and escherichia coli O157: H7, and meanwhile, in fruit and vegetable cleaning application, the effectiveness of the synergistic antibacterial effect of the method is evaluated under a cherry tomato and lettuce system; and the molecular mechanism of sterilization is explored from the aspects of cell membrane morphology and property change at the cellular level, and the synergistic effect mechanism of citric acid and sodium hypochlorite is discussed. The method can be applied to fresh fruit and vegetable surface cleaning and disinfection, and an important theoretical basis is provided for guaranteeing the safety of fresh fruit and vegetable microorganisms.

Visual detection method for multiple pathogens based on top-speed PCR (Polymerase Chain Reaction)

Resumen de: CN120060507A

The invention discloses a multi-pathogen visual detection method based on top-speed PCR (Polymerase Chain Reaction), and belongs to the technical field of molecular biological detection. The invention designs specific primers and molecular beacons of salmonella Sa, vibrio parahaemolyticus VP and infectious subcutaneous and hematopoietic necrosis virus IHHNV for multiple PCR (Polymerase Chain Reaction), and three pathogens can be simultaneously amplified in a PCR system. In addition, the molecular beacon and the target are combined to expose the fluorophore, and the three pathogens can be distinguished without opening a cover by means of a self-made visual device. The multiple visual PCR detection method provided by the invention has the advantages of strong specificity, high sensitivity, short detection time, direct observation and the like, can realize simultaneous detection of multiple pathogens in a single sample, saves the cost, has higher economic value, and is suitable for on-site instant detection.

Detection method of carbapenem-resistant enterobacter (CRE) antibody

Resumen de: CN120058881A

The invention provides an antigen protein. The antigen protein is any one or a combination of more of proteins as shown in SEQ ID NO: 1-5. The antigen protein provided by the invention can be used for detecting the existence of carbapenem-resistant enterobacter (CRE) in a subject. The invention also provides a detection reagent or a kit and a method for detecting or diagnosing carbapenem-resistant enterobacter (CRE) by using the antigen protein, the detection reagent or the kit. The method disclosed by the invention has the advantages of simple material taking, low price, high detection speed, high detection rate and the like, so that the method is very suitable for on-site detection or bedside detection of CRE.

Salmonella manhattan phage and application thereof

Resumen de: CN120060164A

The invention relates to the technical field of microorganisms, in particular to a salmonella manhattan phage and application thereof. The phage is named as vB Sal M467 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.46195, and the preservation date is September 29, 2024. The bacteriophage provided by the invention is separated from sewage of a farm and belongs to a tailed virus, and genomics shows that the bacteriophage belongs to a Kayfunavius genus, a non-virulence gene and a drug-resistant gene, and has safety. The biological characteristic result of the bacteriophage shows that at least eight salmonella serotypes can be split, the bacteriophage has good stability to temperature (-20 DEG C to 60 DEG C) and acid-base property (pH = 3-12), the outbreak amount is 158.83 PFU/cell, and the bacteriophage can be massively proliferated in a short time. The bacteriophage can be used for preventing and controlling salmonella in food such as lettuce and milk.

Preparation process of high-specificity salmonella typhimurium plate agglutination antigen for pullorum disease

Resumen de: CN120064644A

The invention discloses a high-specificity pullorum disease salmonella gallinarum plate agglutination antigen preparation process, which comprises the following steps: respectively resuscitating pullorum disease salmonella C79-1 and C79-7 strains to prepare seed solutions, inoculating a suitable culture medium, carrying out fermentation culture according to a specific process, carrying out centrifugal collection on thalli after inactivation, and carrying out freeze drying to obtain the high-specificity pullorum disease salmonella gallinarum plate agglutination antigen. The preparation method comprises the following steps: carrying out high pressure at 121 DEG C for 2 hours, washing for multiple times, removing protein antigen components which are easily subjected to cross reaction with escherichia coli or enterobacteriaceae bacteria which are commonly used in other environments, adjusting the C79-1 strain and the C79-7 strain to proper bacterium concentration, adding Tween-20, crystal violet and glycerol, and fully homogenizing to obtain the salmonella typhimurium plate agglutination antigen for pullorum disease. The antigen has higher specificity, and is more accurate when being used for purifying and detecting the pullorum disease; according to the fermentation process, the bacterial yield is 4 times of that of common fermentation and 2 times of that of solid culture, ethanol precipitation is not needed, and the production cost is lower; tween-20 is added, so that the surfa

Construction and application of intratumoral self-proliferation enhanced oncolytic microbial delivery system

Resumen de: CN120053379A

Construction and application of an intratumoral self-proliferation enhanced oncolytic microbial delivery system belong to the technical field of pharmaceutical preparations, a hydrogen sulfide donor is endowed with a membrane-insertable regional structure through a synthetic reaction, then a lipid membrane containing the membrane-insertable hydrogen sulfide donor is coated with bacteria through a self-assembly effect, and the membrane-insertable hydrogen sulfide donor is obtained. An oncolytic microbial delivery system with enhanced intratumoral self-proliferation is prepared. After the delivery system is intravenously injected, the delivery system is enriched in a tumor in a targeted manner, hydrogen sulfide released by a donor can be converted into tetrathionate in a tumor inflammation microenvironment, tetrathionate respiration belongs to specific respiration of salmonella, and the specific respiration belongs to specific respiration of salmonella. The proliferation of a salmonella tumor part is specifically promoted through two respiratory chains of aerobic respiration and tetrasulfate respiration, so that the pyroptosis of tumor cells is enhanced, and the anti-tumor purpose is achieved. The invention provides a brand new theory and an advanced solution for cancer treatment, also provides a promising strategy and exploration thought for clinical application of attenuated salmonella, and has important scientific research value.

Extract of an herbal composition as antimicrobial and/or antibiofilm agent

Resumen de: NZ752690A

An extract of an herbal composition comprising at least two different dried plants useful as antimicrobial and/or antibiofilm agent in the treatment or prevention of microbial infections caused by bacteria, such as for example Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Streptococcus or Staphylococcus, or by fungi, such as for example is herein described. It has been found that in such extract, the active ingredients exert their biological effects in a synergistic manner. The extract may constitute the active ingredient of a food supplement, a nutraceutical, pharmaceutical or cosmetic composition or a functional food or a food additive. A process for preparing said extract is also described here.

Clostridium butyricum CB24011205 and application thereof

Resumen de: CN120060007A

The invention relates to the technical field of microorganisms, in particular to clostridium butyricum CB24011205 capable of inhibiting growth and colonization of vibrio cholerae and application of the clostridium butyricum CB24011205. According to the present invention, the clostridium butyricum CB24011205 strain is separated from the intestinal tract content of the marine white shrimp, and the preservation number of the clostridium butyricum CB24011205 strain is CGMCC No. 33030; the strain has the capability of efficiently inhibiting vibrio cholerae, can be compounded with daidzein to prepare a microecological preparation, not only has remarkable advantages in colonization and growth of vibrio cholerae, but also can provide certain protection for other pathogenic bacteria (such as escherichia coli and salmonella), can be used for enhancing intestinal immunity and repairing functions, and can be used for preparing the microecological preparation. Good application prospects are realized.

Immune micro-fluidic chip for efficiently enriching and detecting escherichia coli O157: H7 and application thereof

Resumen de: CN120064646A

The invention discloses an immune micro-fluidic chip for efficiently enriching and detecting escherichia coli O157: H7 and application of the immune micro-fluidic chip, and belongs to the field of food-borne pathogenic bacterium detection. The immune micro-fluidic chip is constructed by seven layers of plates. The seven layers of plates structurally and sequentially comprise a top plate, a first immune fiber channel layer, a first isolation layer, a second immune fiber channel layer, a second isolation layer, a third immune fiber channel layer and a bottom plate from top to bottom. Coupling the amination modified glass fiber membrane with a monoclonal antibody to successfully prepare an immune fiber membrane; the immune fiber membrane is arranged in a chip channel, and the escherichia coli O157: H7 in food can be rapidly detected on the basis of a double-antibody sandwich and an enzymatic reaction principle. The chip preparation process is simplified, the manufacturing cost is reduced, the time-consuming and tedious pre-enrichment step is avoided through large-volume sample injection, and the innovation is expected to provide important technical support in the fields of food quality control and safety detection such as food production and first-line supervision.

Anti-S11 type riemerella anatipestifer monoclonal antibody and blocking ELISA antibody detection kit and application thereof

Resumen de: CN120058920A

The invention discloses an anti-S11 type riemerella anatipestifer monoclonal antibody as well as a blocking ELISA antibody detection kit and application thereof, and belongs to the technical field of poultry immunology. The monoclonal antibody provided by the invention comprises a heavy chain and a light chain, the heavy chain comprises VHCDR1, VHCDR2 and VHCDR3 of which the amino acid sequences are as shown in SEQ ID NO: 1-3, and the light chain comprises VLCDR1 of which the amino acid sequence is as shown in SEQ ID NO: 4, VLCDR2 of which the amino acid sequence is LVS and VLCDR3 of which the amino acid sequence is as shown in SEQ ID NO: 5; the blocking ELISA antibody detection kit prepared from the monoclonal antibody can specifically detect the level of a riemerella anatipestifer serotype 11 (S11 type) antibody in a sample, has no cross reactivity with riemerella anatipestifer S1 type, riemerella anatipestifer S2 type, riemerella anatipestifer S5 type, riemerella anatipestifer S6 type and riemerella anatipestifer S14 type, avian pathogenic escherichia coli, avian pasteurella multocida and salmonella, and is high in sensitivity and specificity.

RPA primer group and kit for rapidly detecting salmonella pullorum based on RPA-CRISPR/Cas12a

Resumen de: CN120060515A

The invention discloses an RPA primer group and a kit for rapidly detecting salmonella pullorum based on RPA-CRISPR/Cas12a, and belongs to the technical field of salmonella pullorum visual rapid detection. A specific target gene ipaj in a salmonella pullorum genome is taken as a target spot, an RPA primer pair and a specific crRNA sequence are designed and optimized, the salmonella pullorum is detected by combining an RPA isothermal amplification method and a CRISPR/Cas12a detection method, a detection result can be interpreted by naked eyes under blue light, and the detection result is accurate. The kit has the characteristics of high sensitivity, strong specificity, accurate result, short time consumption, simplicity and convenience in operation and high practicability, and is suitable for rapid screening or detection of the pullorum disease on site.

Escherichia coli bacteriophage PEC-36 with cross-species host characteristic and application thereof

Resumen de: CN120060163A

The invention discloses an Escherichia coli bacteriophage PEC-36 with cross-species host characteristics and application of the Escherichia coli bacteriophage PEC-36, and belongs to the technical field of biology. The Escherichia coli bacteriophage PEC-36 is separated from sewage of a pig factory, can simultaneously split O8: H7, O21: H34, O23: H16, O25: H4, O81: H27 and H25 serotype strains of Escherichia coli and fowl typhoid, London, swine cholera and other serotype strains of salmonella, and has a cross-species splitting characteristic. Experiments prove that the bacteriophage has the advantages of high titer, high stability, high safety and the like, and can remarkably inhibit the growth of escherichia coli and salmonella, so that a technical support is provided for research and development of bacteriophage preparations for treating infection related to escherichia coli and salmonella; the invention provides a safe and efficient antibiotic alternative scheme for treating human and animal Escherichia coli and salmonella related infection, and has a good application prospect.

CONTINUOUS FEED, HORIZONTAL WATER SPRAY SHELL EGG PASTEURIZATION SYSTEM

Resumen de: US2025169515A1

A continuous feed, shell egg pasteurization system and method uses sprayed heated water. The unpasteurized shell eggs are conveyed and rotated through a horizontal tunnel. An overlapping pattern of heated water is sprayed downward onto the rotating shells eggs. Verified testing shows that the shell eggs achieve a statistical 5 log reduction of Salmonella enteritidis that may have been present in the yolk in the respective unpasteurized shell egg, while at the same time are not overcooked and have little to no whitening of the albumen.

MDR E. COLI IMMUNOGEN

Resumen de: US2025170230A1

The subject relates to an isolated antibody that specifically binds to O25b antigen of multi drug resistant (MDR) E. coli strains, its medical and diagnostic use, method of producing the antibody, including an isolated nucleotide sequence, plasmids and host cells as used in the production of the antibody; and further an isolated epitope recognized the specific antibody.

Enterococcus faecium capable of simultaneously degrading three mycotoxins and application of enterococcus faecium

Nº publicación: CN120041351A 27/05/2025

Solicitante:

HENAN JINBAIHE BIOTECHNOLOGY CO LTD
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Resumen de: CN120041351A

The invention discloses enterococcus faecium capable of degrading three mycotoxins at the same time and application of the enterococcus faecium, and belongs to the technical field of microorganisms. The enterococcus faecium JBHBIO-BSC1 disclosed by the invention is preserved in the China Center for Type Culture Collection, and the preservation number of the enterococcus faecium JBHBIO-BSC1 is CCTCC (China Center for Type Culture Collection) NO: M 20241918. The enterococcus faecium disclosed by the invention has the most remarkable characteristic that the enterococcus faecium has an obvious degradation effect on the aflatoxin, the vomitoxin and the zearalenone, and the degradation rates of the aflatoxin, the vomitoxin and the zearalenone can respectively reach 74.67%, 85.83% and 82.12%. The enterococcus faecium disclosed by the invention is natural, safe, acid-resistant, alkali-resistant and high-temperature-resistant, and has relatively strong organic acid production capacity. In addition, the strain has an obvious inhibition effect on escherichia coli, salmonella and staphylococcus aureus, and is expected to be applied to the fields of food processing and microbial pharmacy.