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SipD重组蛋白在制备检测禽沙门氏菌抗体的间接ELISA检测试剂盒中的应用

Resumen de: CN119804862A

本发明涉及血清学检测技术领域，特别是涉及SipD重组蛋白在制备检测禽沙门氏菌抗体的间接ELISA检测试剂盒中的应用。本发明通过大量研究，成功筛选出用于检测禽沙门氏菌抗体的特异性抗原‑SipD蛋白。本发明以禽沙门氏菌III型分泌系统SipD亚基(SipD蛋白)为抗原，经蛋白表达纯化后获得包被抗原(SipD重组蛋白)，通过反应条件的摸索与优化，最终建立了灵敏性高、特异性强和重复性好的禽沙门氏菌间接ELISA检测方法。相比于传统的玻片凝集抗原检测方法，该方法极大地提高了检测通量和检测效率。该检测方法的建立对于人类、动物、食品中的沙门氏菌检测具有重要的公共卫生意义。

Primer composition and kit for detecting infectious diarrhea pathogens

Resumen de: CN119799975A

The invention provides a primer composition and a kit for detecting infectious diarrhea type pathogens. Staphylococcus aureus, escherichia coli, klebsiella pneumoniae, proteus mirabilis, listeria monocytogenes, pseudomonas aeruginosa, salmonella enteritidis, clamping film bacillus aerogenes, vibrio cholerae, clostridium difficile, aeromonas capora, yersinia enterocolitica, plesiomonas shigelloides, vibrio parahaemolyticus, campylobacter jejuni, candida albicans and aspergillus fumigatus are detected at the same time. The kit can be one or any combination of Aspergillus flavus, entamoeba histolytica, human adenovirus A, human adenovirus B, human adenovirus C, human adenovirus F, human bocavirus, rotavirus, norovirus, fiveleaf virus and human astrovirus, and has the advantages of short time consumption, high resolution, high flux, high sensitivity and the like.

Phage composition for preventing and treating salmonella abortus of horses, biological preparation and application of bacteriophage composition and biological preparation

Resumen de: CN119799656A

The invention belongs to the technical field of biology, and particularly relates to a bacteriophage composition for preventing and treating horse salmonella abortus, a biological preparation and application of the bacteriophage composition and the biological preparation. The bacteriophage composition comprises a bacteriophage 4FS1, a bacteriophage DS-BP2 and a bacteriophage DS2; the preservation number of the bacteriophage 4FS1 is CCTCC (China Center For Type Culture Collection) NO: M 20242020; the preservation number of the bacteriophage DS-BP2 is CCTCC (China Center For Type Culture Collection) NO: M 202402021; the preservation number of the bacteriophage DS2 is CGMCC (China General Microbiological Culture Collection Center Three kinds of virulent bacteriophages 4FS1, DS-BP2 and DS2 aiming at different host spectrums of salmonella are separated and screened, the three kinds of virulent bacteriophages 4FS1, DS-BP2 and DS2 are combined into the bacteriophage cocktail, the occurrence of bacteriophage-tolerant strains can be effectively prevented or delayed, and the screened bacteriophages have a synergistic interaction effect; the three bacteriophages obtained by separation can be independently used or combined into a bacteriophage cocktail for preventing or treating infection caused by horse abortion salmonella, such as female donkey abortion and the like.

Fusion protein of salmonella typhimurium flagellin derivative and Seneca virus antigen and application thereof

Resumen de: CN119798461A

The invention belongs to the technical field of biology, and discloses a fusion protein of a salmonella typhimurium flagellin derivative and a Seneca virus antigen and application of the fusion protein. A salmonella typhimurium flagellin derivative gene and a Seneca virus antigen gene are fused, codon optimization is carried out, the fusion protein is cloned to a pET-28a (+) vector, an escherichia coli system is utilized to express the fusion protein, and in-vivo experiments prove that the fusion protein shows good biological activity, can activate the activity of a TLR5 ligand and promote the activation of macrophages, and can be used for preparing a recombinant vector. And a foundation is laid for the development of Seneca virus subunit vaccines.

Traditional Chinese medicine composite lactobacillus acidophilus NM probiotic preparation for improving oral bacterial flora and preparation process of traditional Chinese medicine composite lactobacillus acidophilus NM probiotic preparation

Resumen de: CN119792444A

The invention discloses a traditional Chinese medicine composite lactobacillus acidophilus NM probiotic preparation for improving oral bacterial flora and a preparation process thereof, and relates to the related field of biotechnology. According to the traditional Chinese medicine composite lactobacillus acidophilus NM probiotic preparation, the synergistic effect of probiotics and traditional Chinese medicine is combined, the traditional Chinese medicine composite lactobacillus acidophilus NM probiotic preparation has the multiple advantages of regulating oral flora, resisting bacteria and diminishing inflammation, resisting oxidation, relieving fatigue and the like, lactobacillus acidophilus NM can tolerate acid and cholate, pathogenic bacteria such as escherichia coli, staphylococcus aureus and salmonella are effectively inhibited, and the traditional Chinese medicine composite lactobacillus acidophilus NM probiotic preparation is suitable for oral administration. The traditional Chinese medicine composition has the advantages that the traditional Chinese medicine composition can be used for reducing dental caries, gingivitis and ozostomia, is free of antibiotic drug resistance and is safer after being used for a long time, radix scutellariae, honeysuckle and green tea extracts are rich in flavonoids and tea polyphenols and can further resist bacteria and inflammation and improve oral health, and meanwhile, the probiotics can reduce blood lactic acid, blood urea nitrogen

一种减毒沙门氏菌来源的细菌外膜囊泡蛋白载药系统及其制备方法和应用

Resumen de: CN119792228A

本发明公开了一种减毒沙门氏菌来源的细菌外膜囊泡蛋白载药系统及其制备方法和应用，本发明利用细菌外膜囊泡蛋白载药系统可以在细菌外膜囊泡表面实现蛋白药物的“即插即用”。本发明构建了细菌外膜囊泡蛋白载药系统。本发明敲除了减毒鼠伤寒沙门氏菌VNP20009的OmpA，并回补在胞外环结构域中插入SpyTag的重组OmpA，利用SpyTag/SpyCatcher间自发形成异肽键的性质，将SpyCatcher与蛋白药物的融合蛋白展示在细菌外膜囊泡表面，制备方法简单，容易操作，在细菌外膜囊泡表面展示多种蛋白，并高效保护和负载蛋白药物。结合细菌外膜囊泡的免疫刺激特性，实现肿瘤的高效治疗。

Salmonella-resistant nano material screening system

Resumen de: CN119811555A

The invention discloses an anti-salmonella nanometer material screening system. The system comprises the following steps: designing a candidate material structure through molecular dynamics simulation; performing comparative observation on the synthetic material and antibiotics, and collecting drug sensitivity test images to form a data set; training a computer vision model to automatically distinguish the antibacterial effect; carrying out high-throughput automatic testing on the candidate material by using a microfluidic platform; efficient screening is carried out according to the test effect; the system can realize automatic mining of the anti-salmonella nanometer material.

IMPROVED SALMONELLA VECTORED THERAPIES FOR TREATMENT OF CANCER

Resumen de: WO2023235633A2

A genetically modified Salmonella cell (GMSC) engineered to exhibit specific targeting to cells and regulated delayed lysis in vivo, the GMSC comprising a first heterologous nucleic acid that encodes a first gene product that causes the GMSC to be selectively localized to and/or internalized by a target cell in vivo and a second heterologous nucleic acid that encodes a second gene product that facilitates killing of the target cells following internalization.

METHOD FOR IDENTIFYING ESBL ENZYMES IN ENTEROBACTERIA

Resumen de: WO2023227848A1

The present invention relates to a method for determining the properties of resistance to beta-lactam antibiotics of a strain of enterobacteria present in a biological sample, which method comprises the steps of: a) lysis of the bacteria and hydrolysis of the bacterial proteins in order to obtain a mixture of peptides, b) analysis of this mixture of peptides by targeted mass spectrometry coupled with reverse phase liquid chromatography, detection of at least one specific peptide of an enzyme exhibiting extended spectrum beta-lactamase (ESBL) activity being indicative of the resistance to beta-lactams of the strain of enterobacteria present in the biological sample, the method being characterised in that the analysis step (b) is carried out in the presence of an ion pairing reagent (IPR) added into the chromatography column or into the mixture of peptides.

一种感应铁镁离子的细菌传感器的构建方法及其应用

Resumen de: CN119776251A

本发明公开了一种感应铁镁离子的细菌传感器的构建方法及其应用。本发明属于微生物领域，具体涉及一种感应铁镁离子的细菌传感器的构建方法及其应用。重组大肠杆菌含有超折叠绿色荧光蛋白sfGFP编码基因、感应蛋白PmrB突变体编码基因和响应调节蛋白PmrA突变体编码基因、信号传导蛋白PmrD编码基因和强核糖体结合位点RBS，并含有通过调节蛋白PmrA激活的磷酸化酶PmrG的启动子驱动的T7 RNA聚合酶编码基因和T7启动子驱动的sfGFP编码基因，同时过表达磷酸乙醇胺转移酶EptA编码基因。本发明构建了检测铁离子浓度的细菌传感器，并可通过铁镁离子的诱导改变细菌传感器的脂多糖的修饰，增强细菌的耐药性。

Bacillus velezensis GY1 and application thereof

Resumen de: CN119776182A

The invention discloses bacillus velezensis GY1, which is preserved in the Guangdong Microbial Culture Collection Center at June 28, 2024, and the preservation number is GDMCC No: 64792. The bacillus velezensis GY1 has the advantages that the bacillus velezensis GY1 is named as GDMCC NO: 64792, and the bacillus velezensis GY1 is named as GDMCC NO: 64792; the bacillus velezensis GY1 can degrade 100% of nitrogen and 56.4% of nitrogen in an M9 culture medium containing 200 mg/L of ammoniacal nitrogen and 1000 mg/L of ammoniacal nitrogen respectively, and the ammonia reduction rate of the bacillus velezensis GY1 in actually produced culture water reaches 53.3%. The compound has a relatively strong inhibition effect on common pathogenic bacteria on aquatic products, such as vibrio alginolyticus, salmonella and vibrio parahaemolyticus.

Composite plant essential oil and emulsion for antagonizing bacterial biofilm as well as preparation method and application of composite plant essential oil and emulsion

Resumen de: CN119770542A

The invention belongs to the technical field of biology, and particularly discloses a composite plant essential oil for antagonizing a bacterial biofilm, which is composed of cinnamon essential oil and star anise essential oil. The compound plant essential oil disclosed by the invention consists of the cinnamon essential oil and the star anise essential oil which have a synergistic effect, have good bacteriostatic activity on salmonella, escherichia coli and the like, can inhibit the formation of a bacterial biofilm, and have a strong removal effect on the formed bacterial biofilm; and when being used for removing bacterial biofilms, the cleaning agent has the characteristics of good removal effect, low toxicity and the like.

Antibacterial peptide CL18 and application thereof

Resumen de: CN119775364A

The invention discloses an antibacterial peptide CL18 and application thereof, and the amino acid sequence of the antibacterial peptide CL18 is shown as SEQ ID NO.1. The antibacterial peptide disclosed by the invention is strong in antibacterial ability, and can play a role in bacteriostasis at a relatively small concentration. In addition, the antibacterial peptide provided by the invention is not influenced by the drug resistance of salmonella, has an obvious inhibition effect on salmonella, and can effectively prevent and control salmonellosis. The antibacterial peptide disclosed by the invention is prepared into an antibacterial drug, a feed additive or a feeding material, and has important significance on healthy development of livestock and poultry breeding industry.

Lactobacillus arrhizatae and application thereof

Resumen de: CN119752718A

The invention discloses lactobacillus arrhizatae and application thereof, and relates to the technical field of microorganisms. The lactobacillus arrhizatae is preserved in the Guangdong Microbial Culture Collection Center on December 10, 2024, the preservation number is GDMCC 65603, and the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. According to the invention, a strain of Lactobacillus argenteus is screened from healthy free-ranging adult local partridge chickens from Guangxi Zhuang Autonomous Region Tibetan City, the strain is named as LB19, and verification proves that the Lactobacillus argenteus has a good inhibition effect on food-borne pathogenic bacteria including salmonella and escherichia coli, and can be used for preparing food-borne pathogenic bacteria. Good application potential is realized in the aspect of preventing and treating food-borne pathogenic bacteria.

Lactobacillus paracasei with antibacterial effect and application of lactobacillus paracasei

Resumen de: CN119752739A

The invention relates to the technical field of microorganisms, and discloses lactobacillus paracasei with an antibacterial effect and application of the lactobacillus paracasei, and the preservation number of the lactobacillus paracasei is CGMCC (China General Microbiological Culture Collection Center) No.33154. The strain has good gastric acid resistance, cholate resistance and strong self-aggregation and hydrophobicity, has a remarkable inhibition effect on staphylococcus aureus and salmonella, and in addition, when the strain is used for preparing the fermented butter, the variety and content of flavor substances in the fermented butter can be increased, so that the fermented butter has the characteristics of prominent cream fragrance, strong flavor and the like. Meanwhile, the lactic acid flavor and the fruit flavor are coordinated, and particularly, the single cream is fermented under the low-temperature condition (10-20 DEG C), so that the prepared fermented butter is richer in variety and content of flavor substances.

CRISPR/Cas12a micro-fluidic chip aptamer sensor based on G4 dimer enhancement and application of CRISPR/Cas12a micro-fluidic chip aptamer sensor

Resumen de: CN119757745A

The invention provides a CRISPR (clustered regularly interspaced short palindromic repeats)/Cas12a (CRISPR/Cas12a) micro-fluidic chip aptamer sensor based on G4 dimer enhancement and application of the CRISPR/Cas12a The sensor adopts a three-layer PMMA (Polymethyl Methacrylate) structure and integrates a replaceable self-assembled pre-stored reagent tube and a contraction-expansion micro-mixing channel with a rhombic micro-column, so that the rapid, sensitive and specific detection of salmonella typhimurium is realized; wherein the rhombic micro-column design of the micro-mixing channel can generate asymmetric vortexes, so that the fluid mixing efficiency is enhanced; the self-assembly pre-storage reagent tube realizes automatic addition of the reagent, so that the chip can be repeatedly used. The detection range of the sensor is 3.6 * 10 < 1 >-3.6 * 10 < 6 > CFU/mL, the detection limit is 36 CFU/mL, and the recovery rate of the sensor in actual samples such as milk, pre-cut vegetables and chicken is 88.11-105.19%. According to the present invention, the detection sensitivity is improved through the fluorescence enhancement effect of the G4 dimer, the automation and the high efficiency of the detection process are achieved by combining the microfluidic technology, and the method is suitable for the food safety detection field.

Fusion plasmid and application thereof in gene transfer and protein expression

Resumen de: CN119752974A

The invention relates to the technical field of biology, in particular to a fusion plasmid and application thereof in gene transfer and protein expression. The invention provides brand new IncI1 and IncHI2/IncHI2A fusion plasmids which are derived from clinical salmonella, contain NDM-13 and OXA-10 at the same time and can be transferred autonomously, and the plasmids also carry other various drug-resistant genes and show drug resistance to various antibiotics. The salmonella is a pathogenic bacterium of zoonosis and has the capacity of infecting people and livestock. The salmonella carrying the plasmid has the potential of outbreak prevalence, and needs to be positively researched to prevent the outbreak prevalence.

Water-soluble dimetridazole composition as well as preparation method and application thereof

Resumen de: CN119745871A

The invention provides a water-soluble dimetridazole composition as well as a preparation method and application thereof, and belongs to the technical field of veterinary drug preparation. The composition comprises 10%-20% of dimetridazole, 2%-4% of tremella oligosaccharide, 2% of vitamin k3, 0.4%-1.2% of hydroxypropyl methylcellulose HPMC E5, 0.1%-0.4% of xanthan gum, 15%-25% of a cosolvent, 5%-10% of a stabilizer, 2% of Tween 20 and the balance of a water-soluble carrier. The composition can be dissolved in 8000 ppm of water and suspended in 12000 ppm, can be uniformly dispersed without layering within 4 hours, and is convenient for drinking water administration. The dimetridazole and the tremella oligosaccharide are combined, so that the prevention and treatment effect on pathogenic bacteria such as escherichia coli and salmonella is enhanced, the use amount of antibiotics and traditional Chinese medicine extracts is reduced, and the bioavailability and efficacy of the medicine are improved. The composition has an exact curative effect on poultry enteritis and salpingitis caused by the pathogenic bacteria, and is suitable for being applied to intensive large-scale farms.

Antibacterial peptide for normal-temperature preservation of boar semen as well as preparation method and application of antibacterial peptide

Resumen de: CN119751588A

The invention discloses an antibacterial peptide for normal-temperature preservation of boar semen as well as a preparation method and application of the antibacterial peptide. The amino acid sequence of the sequence of the antibacterial peptide C8-TAT9 is as shown in SEQ ID No. 1. The antibacterial activity of the obtained antibacterial peptide, the bacterial growth inhibition capability of the antibacterial peptide in normal-temperature preservation of semen and the influence on the motility and activity of sperms are found, and the antibacterial peptide C8-TAT9 not only has the antibacterial activity on escherichia coli, salmonella, pseudomonas aeruginosa, staphylococcus aureus, staphylococcus aureus and the like, but also has the antibacterial activity on sperms. Gram-negative bacteria such as staphylococcus epidermidis, enterococcus faecalis and the like and gram-positive bacteria can be obviously inhibited, and an efficient inhibition effect on methicillin-resistant staphylococcus aureus can be realized; therefore, the antibacterial peptide C8-TAT9 disclosed by the invention can efficiently inhibit the growth of bacteria when the semen is stored at normal temperature, and has no obvious influence on the motility rate of the pig sperms.

一株马流产沙门菌crp基因缺失株及其构建方法和作为减毒疫苗候选株的应用

Resumen de: CN119752748A

本发明属于兽医生物制品领域，具体涉及一株驴源马流产沙门菌crp基因缺失株YZUSAL01Δcrp及其构建方法和应用。本发明的基因缺失株已保藏于中国典型培养物保藏中心，保藏号为CCTCC NO:M 20241741。该缺失株是利用λ‑Red同源重组技术，将马流产沙门菌的crp基因敲除后获得的。本发明制备的基因缺失株毒力显著下降，具有良好的安全性。对小鼠的攻毒免疫保护效果良好，为马流产沙门菌减毒活疫苗奠定基础。

Attenuated salmonella-based EN-VNP outer membrane vesicle nano delivery system and preparation method and application thereof

Resumen de: CN119746056A

The invention discloses a nano delivery system based on attenuated salmonella EN-VNP outer membrane vesicles as well as a preparation method and application of the nano delivery system. The nano delivery system based on the attenuated salmonella EN-VNP outer membrane vesicles comprises an EN-VNP OMV vesicle carrier EMV, a protein antigen and a connexon, the protein antigen is any one of a bacterial antigen, a virus antigen or a tumor antigen; the linker adopts a biotin-streptavidin coupling technology, so that antigen coupling is realized. The invention provides a safe, efficient and stable antigen delivery system, which has a wide application prospect, especially in the fields of vaccine development and tumor treatment.

Parclostridium graciliatum Pt517 and application thereof in preparation of anti-inflammatory or antibacterial products

Resumen de: CN119752719A

The invention relates to the technical field of microorganisms, in particular to paraclostridium graciliatum Pt517 and application thereof in preparation of anti-inflammatory or antibacterial products. The preservation number of the paraclostridium graciliatum Pt517 is CGMCC (China General Microbiological Culture Collection Center) No. 31232. The bacteriostasis comprises the inhibition of escherichia coli, salmonella typhimurium, listeria monocytogenes or staphylococcus aureus. According to the invention, a strain of paraclostridium graciliatum with bacteriostatic and anti-inflammatory capacities is obtained through screening, and the paraclostridium graciliatum has relatively high safety to animals, has the effect of relieving infection symptoms of salmonella typhimurium, provides a new strategy for prevention and treatment of salmonella typhimurium, and has potential clinical application prospects.

Primer and probe for salmonella detection, kit and quantitative detection method

Resumen de: CN119753190A

The invention belongs to the field of gene engineering and biological detection, and provides a primer, a probe, a kit and a quantitative detection method for salmonella detection. By designing a group of specific primers and probes, a detection method which can be widely applied to salmonella detection and is high in specificity, high in sensitivity, good in repeatability, rapid in detection, simple and convenient to operate and low in cost is established, and the detection method can be applied to rapid identification of salmonella and also can be applied to rapid detection of salmonella. And the salmonella in the to-be-detected sample can be subjected to absolute quantitative detection.

Composition for regulating intestinal microenvironment and relaxing bowel and preparation method thereof

Resumen de: CN119745066A

The invention relates to a composition for regulating intestinal microenvironment and relaxing bowel and a preparation method thereof, and belongs to the technical field of biology. The composition comprises the following components in specific parts: artichoke-siraitia grosvenorii-fructus cannabis composite extract peptide, bacillus coagulans-lactobacillus reuteri calcium alginate microspheres, escherichia coli bacteriocin, salmonella bacteriocin, a liquorice extract, a semen cassiae extract, a herba cistanche extract, a dendrobium officinale extract and the like. Wherein the artichoke-siraitia grosvenorii-fructus cannabis compound extract peptide, bacillus coagulans-lactobacillus reuteri calcium alginate microspheres, the liquorice extract, the semen cassiae extract, the herba cistanche extract and the dendrobium officinale extract are obtained by a special preparation method. Through cooperation of the components, the intestinal microenvironment can be adjusted from multiple angles, and the comprehensive effects of improving the intestinal flora structure, inhibiting harmful bacteria, promoting the growth of beneficial bacteria, lubricating the intestinal tract, relieving intestinal inflammation, enhancing the intestinal mucosal barrier function, improving the intestinal immunity and the like are achieved.

CRISPR-CAS-BASED COMPOSITION FOR SALMONELLA DETECTION AND SALMONELLA DETECTION METHOD USING SAME

Nº publicación: US2025109447A1 03/04/2025

Solicitante:

PULMUONE CO LTD [KR]
PULMUONE CO., LTD

Resumen de: US2025109447A1

The present invention relates to a CRISPR-Cas based composition for detecting Salmonella and a method for detecting Salmonella using the same, more specifically, to a composition for detecting Salmonella comprising primers capable of specifically amplifying Salmonella through isothermal amplification, guide RNA, and CRISPR-Cas proteins, and a method for detecting Salmonella using the same.