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130
resultados
Última actualización
17/01/2026 [07:43:00]
Resultados 50 a 75 de 130
Resumen de: CN120888408A
The invention belongs to the technical field of microorganisms, and discloses chlorella sorokiniana M6, application of the chlorella sorokiniana M6 and a method for synchronously synthesizing xanthophyll and protein. The preservation number of the Chlorella sorokiniana M6 provided by the invention is GDMCC NO.66205, the Chlorella sorokiniana M6 has an excellent capability of co-producing lutein and protein, and is almost free of chlorophyll synthesis, so that the Chlorella sorokiniana M6 has very important economic value and practical significance for realizing commercial application of microalgae protein and lutein production.
Resumen de: CN120888410A
The invention belongs to the technical field of preparation of extracts, and particularly relates to a preparation method of a microalgae extract. The preparation method of the microalgae extract comprises the following steps: (1) adding sterile water into microalgae in a logarithmic phase to obtain a microalgae mixed solution; (2) spraying the microalgae mixed solution obtained in the step (1) on the surface of a culture carrier, then adding the culture carrier into a microalgae culture medium added with ammonium niobate for culture, and collecting a product; and (3) adding the product in the step (2) into an ethanol water solution, heating, refluxing and extracting to obtain an extracting solution, and concentrating and drying the extracting solution to obtain the microalgae extract. The culture carrier is used in the microalgae culture process, and the ammonium niobate component is added into the microalgae culture medium, so that the content of protein and polysaccharide in the prepared microalgae extract is obviously increased.
Resumen de: CN120884926A
The invention provides a microalgae lipid extraction method using triple-effect cell wall degrading enzyme synergy and ultrasonic assistance, which comprises the following steps: suspending microalgae powder in a buffer solution containing triple-effect cell wall degrading enzyme, and carrying out enzymolysis to obtain a microalgae enzymolysis mixed suspension; the triple-effect cell wall degrading enzyme comprises cellulase, pectinase and mannase; carrying out ultrasonic treatment on the microalgae enzymolysis mixed suspension in an ice bath, centrifuging, discarding supernatant, and collecting precipitated biomass to obtain wall-broken algae powder; the ultrasonic treatment adopts a pulse mode; mixing the wall-broken algae powder with a mixed solvent of n-hexane and isopropanol, extracting, centrifuging to take supernate, and evaporating and concentrating to obtain the lipid extract. A triple-effect cell wall degrading enzyme system is adopted to precisely degrade a polysaccharide network of microalgae cell walls through a synergistic effect; secondly, ultrasonic treatment is carried out under the ice-bath low-temperature condition, the algal alkene hydrophobic barrier on the outer layer of the microalgae cell wall can be efficiently and selectively damaged, and finally the lipid extraction rate is increased.
Resumen de: CN120888409A
The invention discloses application of cationic polymer compounded bentonite flocculation for harvesting wide-salinity microalgae and a harvesting method, and belongs to the technical field of environmental protection. The method comprises the following steps: adding a polydimethyldiallylammonium chloride solution and a sodium bentonite suspension into an algae solution according to the cell biomass of microalgae to be harvested, stirring, settling and collecting to finish harvesting. According to the method disclosed by the invention, the microalgae in the wide-salt environment are harvested by combining PDDAC and BE for flocculation, the microalgae can be efficiently flocculated and harvested within the range of 0-40% of water salinity, and the harvesting rate can reach 90% or above; the flocculating efficiency is high, the dosage of a flocculating agent is small, the floc is large in size and compact, and the salt-resistant characteristic is remarkable; the method is suitable for efficient harvesting of microalgae in artificial culture or natural water under a wide salinity condition, provides a key technology for the field of microalgae harvesting, and has a wide application prospect; the problems of poor salinity tolerance of a flocculating agent, large dosage of the flocculating agent and poor harvesting effect in microalgae harvesting through flocculation at present are solved.
Resumen de: CN120884086A
The invention belongs to the technical field of microalgae biology, and particularly relates to a preparation method of euglena fermentation product filtrate with an anti-oxidation effect. According to the present invention, the patent algae species are fermentation algae species, fermentation and extraction are performed under the special process, and the freezing clarification method and the physical sterilization method are combined, such that the unpleasant odor and the strong color in the euglena fermentation filtrate are effectively removed, and the retention of the active substances in the filtrate is ensured; and an excellent and reliable raw material source is provided for deep utilization and innovative development of subsequent related products. In addition, the euglena fermentation filtrate is non-toxic, harmless and pollution-free, meets the environmental protection standard, contains a large amount of active ingredients, and is a preferred raw material for food and medicine development.
Resumen de: CN120888377A
The invention provides a bioreactor for microalgae culture. The bioreactor comprises a base, a culture bin fixedly installed on the base and an inner light supplementing assembly installed in the culture bin. The inner light supplementing assembly comprises a light supplementing pipe arranged in the center of the culture bin, and the light supplementing pipe is transparent and foldable or telescopic; a telescopic screw rod and an adjusting threaded pipe which is in threaded connection with the telescopic screw rod are arranged in the light supplementing pipe; the telescopic screw is fixedly connected to the inner top end of the light supplementing pipe; an adjusting motor used for driving the adjusting threaded pipe to rotate is installed in the base. The base is provided with an incidence channel for an external light source to enter, and the light source passes through the incidence channel, then is conducted into the light supplementing pipe and then is scattered into the culture bin. According to the device, the light supplementing pipe is arranged in the culture bin, and an external light source enters the culture bin after passing through the light supplementing pipe, so that microalgae in the center in the culture bin can obtain more sufficient illumination, the growth and reproduction of the microalgae are more facilitated, and the biomass concentration of the microalgae is improved.
Resumen de: CN120888540A
The invention provides a microbial composition for realizing nitrogen reduction and synergy of a flooded rice field as well as a preparation method and application of the microbial composition, and belongs to the technical field of rice field nitrogen reduction. The microbial composition disclosed by the invention comprises cyanobacteria microspheres and pseudomonas fluorescens microspheres, wherein the mass ratio of the cyanobacteria microspheres to the pseudomonas fluorescens microspheres is (4.8-5.2): (2.8-3.2); the cyanobacteria microspheres and the pseudomonas fluorescens microspheres are independently packaged. The cyanobacteria microspheres are spread on the water surface of a rice field 7-10 days before rice transplanting; in the early tillering stage of the rice, the pseudomonas fluorescens microspheres are applied to a 5-10cm soil layer at the rhizosphere of the rice, so that the nitrogen loss amount of the flooded rice field is reduced, the nitrogen fertilizer utilization rate and the rice yield of the rice are improved, and a technical support is provided for ensuring the stable yield and yield increase of the rice under the condition that the nitrogen fertilizer application amount of the flooded rice field is reduced.
Resumen de: CN120866027A
The invention belongs to the technical field of microalgae carbon sequestration, and particularly relates to a light energy weight self-adaptive microalgae internal and external light source complementary carbon sequestration system, which comprises a light culture assembly made of a transparent material and used as a container for containing algae liquid; the light supplementing assembly is arranged in the light culture assembly; the detection system is used for detecting illumination intensity data, illumination angle data, algae liquid biomass concentration data and light field distribution data in the light culture assembly; the control system is electrically connected with the detection system and the light supplementing assembly, and the control system adjusts the illumination intensity of the light supplementing assembly according to the illumination intensity data, the illumination angle data, the light field distribution data and the algae liquid biomass concentration data. According to the system, the light quality and the light intensity of a light source are accurately regulated and controlled by predicting the light field distribution in the light culture assembly and adjusting the light supplementing intensity of the light supplementing assembly, so that light field reconstruction is realized, stable carbon sequestration of microalgae is assisted, and high-quality target carbon neutralization is realized.
Resumen de: CN120866073A
The invention discloses a high-efficiency biological carbon sequestration method and system device for coupling culture of microalgae based on flue gas and by-products after ammonia decarburization, and the method is characterized in that the flue gas (the temperature is 25-35 DEG C, and the CO2 concentration is 1-5%) after the ammonia decarburization is directly used as a microalgae carbon source, and the by-product ammonium sulfate (the adding concentration in a culture medium is 0.1-2.5 g/L) after the ammonia decarburization is used as a nitrogen source; ammonium bicarbonate (the addition concentration in the culture medium is 0.1-2.5 g/L) is used as a nitrogen source and an auxiliary carbon source, microalgae are inoculated into the prepared culture medium, the flue gas decarbonized by the ammonia process is directly introduced, and photosynthetic culture is carried out. The pH value of a culture medium is monitored in real time, the flow of flue gas obtained after ammonia decarburization and the adding proportion of ammonium bicarbonate and ammonium sulfate are cooperatively fed back and regulated, the pH of a culture system is kept stable, carbon and nitrogen sources are sufficient, high-precision stable control over the culture environment is achieved, and efficient biological carbon sequestration is achieved.
Resumen de: CN120859151A
The invention provides a preparation method of full-nutrient fresh algae gel for 3D printing, which comprises the following steps: mixing and dissolving starch, a rheological property changing agent, nutritive salt required by microalgae growth and boiling water, adding microalgae cells, and uniformly stirring to form bio-ink for 3D printing. The full-nutrition fresh algae gel not only can support efficient culture of microalgae, but also can be directly used as a full-nutrition fresh algae gel functional food after being subjected to microalgae culture due to the full-food-grade raw materials of the full-nutrition fresh algae gel.
Resumen de: CN120866369A
The invention belongs to microalgae bioengineering, and particularly relates to an MCP transgenic expression vector and application thereof. The invention provides an MCP recombinant plasmid, the MCP recombinant plasmid is transferred into microalgae for the first time, the microalgae is not only an initial feed but also can be used as an expression vector of an exogenous gene, and the MCP gene of DIV1 is used for preparing a microalgae oral vaccine, so that the vacancy of a medicine for preventing and treating the full-eye iridovirus 1 at present is made up, the homology of medicine and food is realized, and the aim of preventing and treating the full-eye iridovirus 1 is fulfilled. The invention provides an environment-friendly, safe and effective transgenic microalgae vaccine drug, promotes the development of an aquatic vaccine technology, and has far-reaching significance on the stable and sustainable development of global aquaculture industry.
Resumen de: CN120866072A
The invention discloses a culture medium and a culture method for inhibiting microalgae sensitization protein expression. The culture medium comprises the following components: an organic carbon source, a nitrogen source, a phosphorus source and other elements, the culture method comprises the following steps: inoculating algae liquid activated to a logarithmic phase into the culture medium for continuous culture, and adjusting the temperature, illumination intensity, light-dark cycle and culture period of the closed photobioreactor for culture. According to the culture medium composition, element matching of the culture medium is ingeniously coordinated, chromatin remodeling and metabolism reprogramming of the microalgae can be effectively influenced, then carbon flow distribution in glycolysis and tricarboxylic acid circulation is changed, energy metabolism and growth balance are rebuilt, and finally inhibition of sensitization protein expression of the microalgae is achieved. On the basis, microalgae sensitization risk factors are determined, and the expression degree of microalgae sensitization protein is reduced by changing the culture condition combination, so that the edible safety of microalgae is improved.
Resumen de: CN120866149A
The invention discloses a cyanobacteria bottom mud fermentation methanogenic bacterial agent, which comprises clostridium butyricum, bacillus amyloliquefaciens and methanosarcina martensii, and is characterized in that the clostridium butyricum accounts for 30-60% of the total number of bacterial cells, the bacillus amyloliquefaciens accounts for 25-50% of the total number of the bacterial cells, and the methanosarcina martensii accounts for 30-60% of the total number of the bacterial cells. The methanosarcina marzeberi accounts for 10%-25% of the total number of bacterial cells. The advanced technology of adding microbial compound bacteria and combining high-temperature and high-pressure hydrolysis and anaerobic digestion technologies is utilized, the biodegradability is improved by destroying the cell structure of organic matter, the efficiency and stability of traditional anaerobic fermentation are remarkably improved, the VS removal rate after pyrohydrolysis fermentation reaches 67.5%-70.4%, the gas production rate in the anaerobic fermentation process after pyrohydrolysis is effectively increased, and the production cost is reduced. The yield of methane can be increased, the water body environment can be improved, recycling of bottom mud resources can be promoted, and green and sustainable ecological restoration is achieved.
Resumen de: CN120866251A
The invention discloses a beta-carotene hydroxylase mutant as well as a preparation method and application thereof, and belongs to the technical field of microalgae gene engineering. Amino acid Cys No. 191 of haematococcus pluvialis beta-carotene hydroxylase is mutated into Ala, amino acid Thr No. 213 of haematococcus pluvialis beta-carotene hydroxylase is mutated into Ala, and the yield of astaxanthin is increased by 1.282 times and 1.268 times respectively. According to the mutant obtained through mutation, the catalytic efficiency and substrate selectivity of beta-carotene hydroxylase are remarkably improved, astaxanthin is produced more specifically through enzymatic reaction, and a key mutation direction is provided for construction of engineering strains for high yield of astaxanthin.
Resumen de: CN120870119A
The invention provides an in-situ online observation device applied to a microalgae growth dynamic response process. The in-situ online observation device comprises an in-situ online observation device and an automatic shooting system, the in-situ online observation device comprises a culture observation plate, a mainstream micro-channel and a micro-culture chamber; the culture observation plate is transparent; the mainstream micro-channel is arranged on the upper side of the culture observation plate; a flow inlet and a flow outlet are respectively formed in two ends of the main flow micro-channel in the length direction; the plurality of micro-culture chambers are communicated with the main flow micro-channel; the plurality of micro-culture chambers are arranged between the flow inlet and the flow outlet; the automatic shooting system is used for shooting microalgae in the micro-culture chamber; the in-situ online observation device is simple and effective in structure and convenient to manufacture, and has the characteristics that internal nutrients and metabolic oxygen are efficiently transported and transferred, and the nutrients are continuously, stably and uniformly distributed; and in combination with an automatic shooting system, long-time continuous imaging observation can be carried out on the intrinsic dynamic response process of the growth of a plurality of microalgae cells, and theoretical guidance is provided for the maximum growth potential of the microalgae c
Resumen de: CN120858911A
The invention discloses an ecological bait production method based on a food chain, which comprises the following steps: S1, mixing wine brewing enzyme liquid and culture tail water in proportion, and stirring to form a nutrient medium required by microalgae growth; s2, conveying the nutrient medium into a microalgae culture module, and regulating and controlling illumination and dissolved oxygen conditions; part of microalgae is conveyed to the plankton breeding unit to cultivate plankton to construct a live bait chain; s3, tail water generated by breeding flows back to the plankton breeding unit to serve as a nutrition supplement source of plankton, and closed-loop circulation is achieved.
Resumen de: CN120866183A
The invention discloses an infrared light driven microalgae photosynthetic carbon sequestration method and application, and belongs to the technical field of photosynthetic organism culture.The method comprises the steps that a near-infrared up-conversion nanometer material is dispersed in a microalgae culture medium, and a full-spectrum or near-infrared light source is used for promoting microalgae photosynthetic carbon sequestration; the near-infrared up-conversion nano material is a rare earth nano material with a NaYF4 inert shell structure. According to the method, the overall sunlight utilization efficiency of the microalgae is improved, and the photosynthetic carbon sequestration capacity of the microalgae is finally improved.
Resumen de: CN120866071A
The invention relates to the technical field of microalgae culture, and discloses a microalgae culture method. The invention relates to a microalgae culture method, which comprises the following steps: (1) in the presence of a fat degradation inhibitor, culturing microalgae species to obtain a microalgae solution; (2) separating, adsorbing and degerming the algae solution to obtain a culture solution; and (3) returning the culture solution to the step (1) for reuse. The method can effectively control the generation of organic matters with a growth inhibition effect in the microalgae culture process, improve the production rate and reduce the culture water consumption.
Resumen de: CN120866074A
The invention belongs to the technical field of microalgae biology, and in particular relates to thaurita procumbens and a method for producing fucoxanthin by utilizing the thaurita procumbens. The invention relates to a phalaenopsis palustris which is named PCSM-1 and is preserved in the China Center for Type Culture Collection on January 11, 2024, and the preservation number is CCTCC NO: M 2024076. The content of fucoxanthin in the brachythecium cutescens is as high as 1.58 wt%, and the brachythecium cutescens can grow under both a photoautotrophic condition and a heterotrophic condition. The method comprises the following steps: firstly, performing heterotrophic fermentation on the palmaris cutescens so that the palmaris cutescens is rapidly proliferated and fermented; the method comprises the following steps: firstly, carrying out photoautotrophy and heterotrophy induction to quickly accumulate the fucoxanthin, so that the whole process is short in period and low in energy consumption, the yield of the fucoxanthin is far higher than that of the existing microalgae, and the problems of low production efficiency, high cost and the like of the current fucoxanthin can be solved.
Resumen de: WO2025224659A1
The present disclosure relates to an apparatus for microalgae and/or cyanobacteria cell culture comprising a unitary body formed from a hydrophobic material; and two or more microcapillary channels extending along the unitary body; wherein the inner surface of the microcapillary channels is coated with nutrients suitable for microalgae and/or cyanobacteria cell culture; and wherein the hydrophobic material is transparent to visible light, and to a kit and sensor thereof. A method for culturing microalgae and/or cyanobacteria in an apparatus comprising a unitary body formed from a hydrophobic material and two or more microcapillary channels extending along the unitary body, wherein the hydrophobic material is transparent to visible light is also disclosed.
Resumen de: WO2024132882A1
The invention provides marine microalgal strain of the Ulvales order of the Ulvophyceae class which have utility in the efficient and environmentally beneficial preparation of triacylglycerides and polyunsaturated fatty acids, which can in turn be used for fuels or feeds.
Resumen de: WO2024133379A1
The invention relates to a method for preparing a cyanobacterium extract, preferably spirulina, comprising phycocyanin enriched in trace elements. The invention also relates to an extract obtained by the method according to the invention, to a composition comprising said extract and to the use thereof.
Resumen de: CN120841600A
The invention relates to a microalgae culture solution recycling method, which comprises: carrying out solid-liquid separation on a microalgae culture solution to obtain a filtrate; enabling the filtrate to pass through a resin column and collecting effluent; the effluent is optionally mixed with a supplement and then returned for microalgae culture. By adopting the method for recycling the microalgae culture solution, wastewater discharge in the microalgae culture process can be effectively reduced, the wastewater biochemical treatment pressure is reduced, after the treated effluent is recycled for microalgae culture, the higher microalgae biomass content can still be obtained, the water consumption of microalgae production is remarkably reduced, and the method has a good application prospect.
Resumen de: CN120843281A
The invention relates to the technical field of algae culture, in particular to a microalgae expanding culture method, a microalgae expanding culture system and an ecological restoration method. The microalgae expanding culture method comprises the following steps: inoculating a microalgae seed solution into a first culture solution, and carrying out first amplification treatment under the illumination condition of 100-500lux to prepare a first algae seed mixed solution; inoculating the first algae seed mixed solution into a second culture solution, and carrying out second amplification treatment under the illumination condition of 1000-3000 lux to prepare a second algae seed mixed solution; inoculating the second algae mixed solution into a third culture solution, and performing third amplification treatment at the pH value of 6.5-10 to prepare a third algae mixed solution; inoculating the third algae mixed solution into a fourth culture solution, and carrying out fourth amplification treatment under a stirring condition to prepare a fourth algae mixed solution; wherein the volumes of the first culture solution, the second culture solution, the third culture solution and the fourth culture solution are sequentially increased. According to the expanding culture method, the microalgae grow fast, and the culture period can be effectively shortened.
Nº publicación: CN120843118A 28/10/2025
Solicitante:
NINGXIA UNIV
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Resumen de: CN120843118A
The invention relates to the technical field of crust preparation preparation, in particular to a crust biological preparation, a preparation method and application thereof, and the preparation method comprises the following steps: S1, preparing a bacillus modifier; s2, preparation of a microalgae-biopolymer mixed solution; s3, primary compounding; and S4, compounding to form the agent. Through the synergistic effect of the bacillus modifier and the microalgae-biopolymer mixed solution, the biological crust forming period is remarkably shortened, the defect that natural recovery is slow (dozens of years) is overcome, meanwhile, the crust forms a compact net-shaped structure and strongly bonds soil particles, and the wind erosion rate is reduced by 50%. The freeze-drying process is improved, the biological activity is better reserved, the stability of the preparation is improved, the preparation is convenient to store and transport, the powder which is crushed and sieved (100 meshes) can be directly scattered, and field planting is further accelerated.
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