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CYNOBACTERIA CULTIVATION, SEDIMENTATION, AND UTILIZATION

Resumen de: US2025115501A1

The present invention is related to the fields of bioplastics. In particular, a strain of photosynthetic cyanobacteria (i.e. blue-green algae) having a high growth rate was engineered to overexpress limonene synthase. These engineered cyanobacteria improve auto-sedimentation for biomass harvest and provide for plastic bioremediation capture. Further, machine-learning methods are provided for improving some of the aspects of cultivating cyanobacteria including, but not limited to, assessing real-time light availability and predicting real-time algae growth rates to increase a cyanobacteria biomass.

MODIFIED PROMOTER, EXPRESSION VECTOR, MICROORGANISM, PRODUCTION METHOD FOR SUBSTANCE, MODIFIED CYANOBACTERIA, AND MANUFACTURING METHOD FOR MODIFIED PROMOTER

Resumen de: EP4534667A1

The present disclosure provides a modified promoter with which the amount of expression of a protein-coding gene can be controlled with flexibility. The modified promoter according to the present disclosure is a modified promoter including a base sequence (SEQ ID NO: 1) located upstream of a start codon of a slr1841 gene, the base sequence consisting of 348 bases, or a base sequence having 80% or more homology with the base sequence represented by SEQ ID NO: 1, the base sequence being modified by at least one selected from the group consisting of (i) replacement of one or more bases included in the base sequence with another base, (ii) deletion of one or more bases included in the base sequence, and (iii) insertion of one or more bases into the base sequence.

EXPLOITATION OF WINE PROCESSING WASTE BY MEANS OF INTEGRATED BIOLOGICAL PROCESSES OF ANAEROBIC DIGESTION AND MICROALGAE CULTURE

Resumen de: WO2023233305A1

The present invention discloses a process mode of microalgal cultivation in semi- continuous on wine wastewater with no need to recirculate the microalgal biomass in the reactor, without dilution and sterilisation of the effluent applied as a growth medium. The process was experimentally carried out by semi-continuously applying, without recirculation of the biomass, the cultivation of Chlorella vulgaris on the digestate from the anaerobic co-digestion of winemaking lees and waste activated sludge. The integrated anaerobic digestion-microalgae treatment process is advantageous from a circular economy perspective as it leads to the exploitation of grape processing chain waste, eliminating the economic burden on producers and involving: i) the phyto-depuration of the effluent, ii) the production of bio- methane and iii) the production of biomass containing high value-added by-products (lipids, proteins and starch) that can be used to produce biodiesel, animal feed, nutraceuticals/pharmaceuticals and for producing bioplastics.

A COMPACT MANNER OF GROWING MICROALGAE SUITABLE FOR CARBON SEQUESTRATION AND CREATING A CIRCULAR ECONOMY

Resumen de: AU2023279503A1

There are closed systems of growing microalgae but as they are not able to produce algal biomass economically they are very few and far between and are only used to grow microalgae for very expensive produce. These closed systems also require large space. The result of the lack of economic closed systems is why almost all algal biomass production currently uses raceway ponds. This invention integrates a new compact, very economic and highly productive closed system of growing microalgae including good parameter control with carbon sequestration from say a coal fired power plant or organic waste management in a way that makes the whole process financially attractive and cyclic and if desired it could be made carbon negative. The compact nature of this invention allows algal farms to be sited next to most sources of carbon dioxide.

METHOD FOR PRODUCING MICROALGAE CONCENTRATES WITH LOW VISCOSITY

Resumen de: EP4534644A1

The present invention relates to a method for producing a microalgae concentrate with low viscosity, the method comprising a step of adjusting the pH of a microalgae fermentation broth and a heat treatment step, in which utility costs and investment costs can be reduced in a dehydration and drying step to secure price competitiveness for production and commercialization of dried microalgae products.

Algae membrane reactor, decontamination optimization method, online monitoring method and related equipment

Resumen de: CN119774770A

The invention relates to the technical field of wastewater treatment, and discloses an algae membrane reactor, a decontamination optimization method, an online monitoring method and related equipment.The MAR reactor comprises an aeration system, an illumination system, a microalgae culture system, a membrane separation system, a water production tank, an algae production tank, a water pump and an intelligent control system; the method comprises the following steps: adding a pure microalgae stock solution through an adding pump, aerating and oxygenating through an air blower, stirring an algae-water mixed solution, enabling the amplified algae-water mixed solution to enter a membrane pool, pumping water by a backwashing pump, recycling the water or returning the water to a front-end advanced treatment unit, enabling a concentrated solution of algae to enter an algae box, centrifugally concentrating, storing and transporting as feed; microalgae species capable of efficiently removing specific pollutants in water are directionally cultivated according to production wastewater with different water quality characteristics, and intelligent operation of comprehensively assisting food industry wastewater treatment is achieved through construction of the self-control integrated algae membrane reactor and construction of the cloud management platform.

Microalgae photobioreactor coupled with light guide plate and microporous pipe

Resumen de: CN119776109A

The present invention relates to the technical field of microalgae photobioreactors, and discloses a light guide plate and microporous pipe coupled microalgae photobioreactor, the reactor comprises: a device body unit, which comprises a reactor body, a light guide plate assembly embedded in the side wall of the reactor body, and a hollow microporous pipe assembly; the light source is cooperatively arranged at the end part of the light guide plate assembly; the light guide plate assembly comprises a plurality of sets of light guide plates distributed in parallel, and the ends of the adjacent light guide plates are staggered. One end of the light guide plate extends out of the reactor body and forms an incident end; a flow channel is formed between each light guide plate and the wall surface of the inner cavity of the reactor body; the photobioreactor with a novel structure is designed, the light guide plate with excellent light transmission performance and the hollow microporous pipe are introduced into the culture of the haematococcus pluvialis, and on the premise that the occupied area of the reactor is not increased, the culture operation of the haematococcus pluvialis is simpler and more convenient and the culture operation is efficient through the integrated structure; and the cost is low.

Efficient carbon-sequestration cyanobacteria mutant strain as well as preparation method and application thereof

Resumen de: CN119776240A

The invention belongs to the technical field of biological culture, and particularly relates to an efficient carbon-sequestration cyanobacteria mutant strain as well as a preparation method and application thereof. The preparation method of the high-efficiency carbon-sequestration cyanobacteria mutant strain comprises the following steps: inoculating an improved BG11 culture medium with a synechococcus slender PCC 7942 algae solution, introducing air with the CO2 concentration of 10-20% into the synechococcus slender PCC 7942 algae solution, carrying out continuous illumination at a culture temperature of 30-35 DEG C for evolution for 3-5 cycles, culturing for 5-10 days in each cycle, and when each cycle begins, culturing the synechococcus slender PCC 7942 algae solution with the CO2 concentration of 10-20% in the improved BG11 culture medium to obtain the high-efficiency carbon-sequestration cyanobacteria mutant strain. And adjusting the algae liquid concentration of the synechococcus slender PCC 7942 to the initial inoculation concentration, and obtaining the high-efficiency carbon-sequestration cyanobacteria mutant strain after evolution is finished. The high-efficiency carbon-sequestration cyanobacteria mutant strain obtained by the invention has relatively high growth rate and biological accumulation amount, and can improve the content of high-added-value products such as lipid and protein while realizing high-efficiency carbon sequestration.

Efficient microalgae cultivation device of circulating water system

Resumen de: CN119776107A

The invention relates to the technical field of microalgae cultivation, in particular to an efficient microalgae cultivation device of a circulating water system, which comprises an electro-catalytic reactor used for treating harmful substances in cultivation tail water and a cultivation raceway pond used for enlarging cultivation of microalgae, a biological treatment unit used for preliminary cultivation of microalgae is arranged on one side of the electro-catalytic reactor, the biological treatment unit comprises a bioreactor I, a bioreactor II and a bioreactor III which are arranged in sequence; the section of the bioreactor I is conical, and the bioreactor I is made of plastic. According to the method, harmful substances in the breeding wastewater can be degraded, then the degraded tail water is used for culturing microalgae, then cyclic utilization of nutrient substances is achieved, the breeding water quality can be stabilized, closed-loop circulation of the breeding water can be formed, and the situation that due to the fact that the types of pollutants in the breeding tail water are complex, the breeding water cannot be recycled is avoided. The tolerance of the microalgae to high-concentration harmful substances is limited, so that the normal growth and the purification efficiency of the microalgae are influenced.

Microalgae closed mixotrophic culture method and culture system thereof

Resumen de: CN119776113A

The invention discloses a microalgae closed mixotrophic culture method and a culture system thereof, and relates to the field of microalgae culture, and the method comprises the following steps: step 1, microalgae culture: pouring a culture solution into a culture tank, adding microalgae to be cultured into the culture solution, then fixing a sealing plate at a tank opening of the culture tank through bolts, and carrying out closed culture; step 2, air supply and nutrient solution addition: carbon dioxide is fed into an air distribution pipe through a pipeline by an air pump in an air supply assembly, bubbles are formed from a spray head and are conveyed into a nutrient solution, and part of rising bubbles are gathered by a baffle plate to prolong the retention time of the bubbles in a water body; the first electric telescopic rod drives the arc-shaped telescopic plate to move downwards, the transparent culture tank inside the arc-shaped telescopic plate is exposed, sunlight provides a light source to breed microalgae, the retention time of carbon dioxide in the water body can be prolonged, the utilization rate of carbon dioxide is increased, and the yield of microalgae is increased.

Preparation method and application of phycomycete compound

Resumen de: CN119774769A

The invention relates to the technical field of water treatment, in particular to a preparation method and application of a phycomycete compound. The phycomycete compound is applied to nitrogen and phosphorus removal and surface water treatment. Wherein the chlorella vulgaris and the quadruplex obliquus have strong reproductive capacity and strong adaptive capacity to environment, and absorb various elements such as carbon, nitrogen and phosphorus from water through autotrophy, heterotrophy and polyculture growth; activated sludge containing bacteroides and pseudomonas can degrade complex organic compounds, has metabolic capabilities such as heterotrophic denitrification and the like, and is beneficial to forming a co-culture system with microalgae in the initial culture stage; and nitrifying and denitrifying bacteria are compounded to consolidate the nitrogen removal environment order, so that the water body is effectively purified and the water color is good. The phycomycete compound obtained by the invention not only can be suitable for high-nitrogen simulation wastewater of a laboratory, but also can be applied to in-situ treatment of an actual river channel, has remarkable environmental benefits and has a wide application prospect.

LED spectrum photobioreactor system for microalgae cultivation

Resumen de: CN119776115A

The invention belongs to a photobioreactor in the field of bioengineering, and particularly relates to a photobioreactor system with novel LED spectrum design for microalgae cultivation. Which comprises a reaction tank (used for containing algae liquid and assembling a special spectrum light source transparent pipeline and related equipment), a special spectrum light source transparent pipeline combination acting on a reaction system, and an automatic transparent pipeline cleaning device, and is characterized in that a multi-spectrum light source combination lamp strip is placed in the special spectrum light source transparent pipeline; the multi-spectrum light source combined lamp strip comprises a main light source and an auxiliary light source, and the continuous spectrum design parameters of the main light source are as follows: the peak wavelength is 465-495 nm, the main wavelength is 510-535 nm, and the continuous spectrum range is 380-780 nm; according to the continuous spectrum design of the auxiliary light source, the light absorption peak of the microalgae is used as the peak wavelength, multiple continuous spectrums are formed, and the spectrum range is 380 nm to 780 nm. According to the invention, the problem of spectrum design defects of indoor artificial light culture of microalgae and the problem of wall adhesion of microalgae in a transparent pipeline of a special spectrum light source are solved, the photosynthesis efficiency of microalgae is improved, and th

Application of cadmium selenide in microalgae culture

Resumen de: CN119776252A

The invention relates to application of cadmium selenide in microalgae culture and a method for culturing microalgae, which comprises the step of adding cadmium selenide into a culture system. According to the method, cadmium selenide (CdSe) is added into a microalgae culture system to obtain a CdSe-cyanobacteria hybrid, so that the growth speed and the photosynthetic activity of microalgae are improved. The practice of the invention creates a new path for breaking through the inherent bottleneck of a microalgae natural photosynthetic system and improving the photosynthetic carbon sequestration efficiency.

Application of carbon nitride in microalgae culture

Resumen de: CN119752763A

The invention relates to application of carbon nitride in microalgae culture and a method for culturing microalgae, which comprises the step of adding carbon nitride into a culture system. According to the method, the graphite phase carbon nitride (g-C3N4) material is added into a microalgae culture system to obtain the g-C3N4-microalgae hybrid, so that the growth speed of microalgae and the accumulation speed of biomass are increased, and the yield of target metabolites in microalgae can also be increased. The practice of the invention creates a new path for breaking through the inherent bottleneck of the natural photosynthetic system of cyanobacteria and improving the photosynthetic carbon sequestration efficiency.

Culture method of microalgae with high starch content

Resumen de: CN119752634A

The invention discloses a culture method of microalgae with high starch content, and belongs to the technical field of new food resource mining. Microalgae are cultured in two stages, the ventilation capacity is fixed in the first stage, the ventilation capacity is dynamically adjusted in the second stage, the ventilation capacity in the early stage of culture is 0.75-1.5 L/min, the ventilation capacity is adjusted to 3-6 L/min when the microalgae start to grow in the exponential stage, and the ventilation capacity is adjusted to 0.75-1.5 L/min when the microalgae start to grow in the later stage of the exponential stage until culture is finished. The high-efficiency culture of the microalgae with high starch content is realized by dynamically adjusting the ventilatory capacity of the second culture stage, so that the microalgae cell powder with high starch content is obtained, and the maximum starch content can reach 61% or above. The method has the advantages of short required culture period, simplicity and convenience, no pollution to the environment in the culture process, and important significance for expanding the microalgae as a new resource food and expanding a new starch resource.

Regenerated gas recycling system

Resumen de: CN119746618A

The invention provides a regeneration gas recycling system, which comprises a regeneration gas separator, a regeneration tower, a regeneration tower, a regeneration tower and a regeneration tower, an inlet of the water collecting tank is connected with a liquid outlet of the regenerated gas separator; an inlet of the gas storage tank is connected with a gas outlet of the regenerated gas separator; the microalgae culture tank is used for culturing microalgae; a nutrient solution is stored in the nutrient solution circulating assembly, an outlet of the moisture collecting tank is connected with an inlet of the nutrient solution circulating assembly, and an outlet of the nutrient solution circulating assembly is connected with a liquid inlet of the microalgae culture tank; an outlet of the gas storage tank is connected with an inlet of the carbon dioxide injection assembly, and an outlet of the carbon dioxide injection assembly is connected with a carbon dioxide inlet of the microalgae culture tank, so that the problem that in the carbon capture process in the prior art, water and carbon dioxide in treated exhaust gas are generally discharged together is solved.

Antibacterial peptide and application thereof in juvenile mollusk culture

Resumen de: CN119751617A

The invention provides an antibacterial peptide and application thereof in juvenile mollusk culture. The amino acid sequence of the provided antibacterial peptide is SEQ ID NO: 2. The antibacterial peptide obtained through screening comes from bivalve shellfish, has small toxic and side effects on the bivalve shellfish, is suitable for playing a role in the bivalve shellfish, and has specificity and pertinence on bivalve shellfish culture water pathogenic bacteria. The amino acid sequence of the provided antibacterial peptide is optimized, the antibacterial activity is high, the stability is high, and the expression efficiency in yeast is high. After high expression of the antibacterial peptide, the microcapsule of which the particle size is suitable for filter feeding of the juvenile mollusks is prepared, so that the problem that the juvenile mollusks cannot feed due to direct splashing to culture water in the prior art is solved, and the problems of low microalgae expression efficiency and low antibacterial peptide content are solved.

Efficient resourceful treatment method for microalgae

Resumen de: CN119750795A

The invention discloses an efficient microalgae resourceful treatment method which comprises the following steps: inoculating a microalgae concentrated solution with the dry weight of more than 30mg/L into wastewater to be treated, then respectively adding nano zero-valent iron suspensions with different concentrations at different physiological stages of microalgae, culturing for 14 days to obtain a microalgae culture solution, detecting the indexes of COD, NH4 < + >-N and PO4 < 3->-P in the microalgae culture solution, and determining the content of the microalgae in the wastewater to be treated according to the indexes of COD, NH4 < + >-N and PO4 < 3->-P in the microalgae culture solution. The method comprises the following steps: standing a microalgae culture solution for 9 hours, carrying out self-flocculation settling on microalgae, carrying out centrifugal treatment on the microalgae culture solution subjected to self-flocculation settling, filtering, carrying out freeze drying to obtain microalgae powder, and extracting the microalgae grease by adopting a chloroform-methanol method. According to the method, nano zero-valent iron with different concentrations is added in different growth stages of the microalgae, so that the performance of removing nutrient substances in the wastewater by the microalgae is enhanced, and meanwhile, the flocculation capacity of the microalgae and the accumulation of grease content are promoted.

Method for expressing bacteriophage lyase by using chlorella vulgaris

Resumen de: CN119752635A

The invention discloses a method and an expression system for expressing bacteriophage lyase by using chlorella vulgaris. The microalgae vector takes chlorella vulgaris as an expression vector, and recombinant plasmids are electrically transferred into cell nucleus genes of the chlorella vulgaris. The method is simple, safe and low in cost. The carrier is high in microalgae system conversion rate, and the expressed lyase has good activity to bacteria. The chlorella vulgaris is an edible alga, and has a wide prospect in the subsequent application of oral administration or perfusion. Meanwhile, the fluorescence imaging performance of the microalgae system is outstanding, and possibility is provided for non-invasive tracking and real-time monitoring of the lyase in vivo. The unique characteristics of chlorella vulgaris are utilized, and a feasible thought and method are provided for efficient expression of the lyase.

Method for promoting chlamydomonas reinhardtii selenoprotein gene expression and increasing selenoprotein yield

Resumen de: CN119752633A

The invention discloses a method for promoting expression of a chlamydomonas reinhardtii selenoprotein gene and increasing the yield of selenoprotein. The method comprises the following steps: S1, inoculating a chlamydomonas reinhardtii algal species into a liquid culture medium, and carrying out shake culture under the illumination conditions of 20-30 DEG C and 0-200 mu mol/(m < 2 >. S) until a growth delay period to a stable period to obtain a chlamydomonas reinhardtii culture solution; s2, the chlamydomonas reinhardtii culture solution is taken, sodium selenite and abscisic acid are added, the concentration of the abscisic acid in the culture solution is 10-1000 mu mol/L, and shaking culture is conducted under the illumination conditions of 20-30 DEG C and 0-200 mu mol/(m < 2 >. S). The plant hormone ABA is added in the chlamydomonas reinhardtii selenium-rich culture process, microalgae growth and biomass accumulation are not affected, expression of selenoprotein can be promoted, and when the method is applied to production of selenoprotein-rich chlamydomonas reinhardtii powder, the selenoprotein yield can be greatly increased.

Composite algae cell as well as preparation method and application thereof

Resumen de: CN119751129A

The invention belongs to the technical field of organic fertilizers, and particularly relates to a composite algae cell as well as a preparation method and application thereof. According to the preparation method disclosed by the invention, the stable algae cell with the nanoparticle layer is obtained by co-incubation of the positively charged amino-modified nanoparticles and the algae cell; and mixing and emulsifying a tannic acid aqueous solution and the algae cells with the nano-particle layers to obtain nano-particle algae cells containing lipid membrane layers, culturing under illumination, and carrying out photosynthesis to obtain the composite algae cells with the inner cores carrying oxygen. In addition, the lipid membrane can carry pesticide and other substances, and the antibacterial ability of the composite algae cells obtained by the method is greatly improved; fertilization is conducted in an ultrasonic atomization delivery mode, the mass transfer efficiency of microalgae fertilizer to leaf surfaces is improved, and the fertilizer utilization rate is remarkably increased.

Continuous Flow Reactor for Conversion of Wastewater to Biohydrogen Using Activated Sludge and Microalgae

Resumen de: US2025109414A1

Apparatus 101 for producing a continuous stream of biohydrogen includes a continuous flow stirred tank reactor (CSTR) 12, lighting arrangement 13; ports 14; peristaltic pumps 16; and gas chromatograph (GC) 18. The CSTR 12 includes internal bioreactor chamber 20; a photosynthesizing microorganism inlet stream 22.1 for delivering Chlorella vulgaris; an organic waste inlet stream 22.2 for delivering wastewater activated sludge (WWAS); and a nutrient inlet stream 22.3 for delivering nutrients into the chamber 20; an outlet stream 24; and stirrers for stirring the contents of the chamber. The lighting 13 is provided by lamps 26 for transmitting light through a transparent sidewall of the chamber 20. The ports 14 and CG 18 are for taking measurements from the chamber 20. The pumps 16 control flowrate of the streams 22.1, 22.2, 22.3 and 24 for regulating biohydrogen production from fermentation while Chlorella vulgaris consumes oxygen to avoid biohydrogen conversion into methane.

EXTRACELLULAR VESICLES FROM GENETICALLY-MODIFIED MICROALGAE CONTAINING ENDOGENOUSLY-LOADED CARGO, THEIR PREPARATION, AND USES

Resumen de: US2025108080A1

Provided are compositions containing extracellular vesicles (MEVs) produced in and isolated from genetically-engineered microalgae, which encode cargo, such as nucleic acids and polypeptides. The MEVs are endogenously loaded by the microalgae with the cargo. The MEVs have a variety of applications as a delivery system for therapeutics, including vaccines, anti-cancer therapeutics, diagnostics, and other such uses. The fate of MEVs upon administration depends upon the route of administration.

A METHOD FOR PREPARING AND SELECTING A POLYMERIC FLOCCULANT FOR USE IN HARVESTING MICROALGAE

Resumen de: AU2023350703A1

The present invention relates to a method for harvesting microalgae, the method comprising (i) mixing one or more monomers, a photoinitiator, water and a deoxygenation system to form a mixture; (ii) exposing the mixture to UV light so as to form an aqueous solution of one or more polymers; (iii) introducing the aqueous solution of one or more polymers to a suspension comprising microalgae in an amount sufficient to produce flocculated microalgae; and (iv) harvesting the flocculated microalgae. The present invention also relates to a method for preparing and selecting a polymeric flocculant for use in harvesting microalgae and a related apparatus.

COMPOSITION FOR MAINTAINING OR INCREASING FIBROUS STRUCTURAL PROTEIN

Nº publicación: WO2025070726A1 03/04/2025

Solicitante:

ROHTO PHARMACEUTICAL CO LTD [JP]
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