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88
resultados
Última actualización
03/11/2025 [07:08:00]
Resultados 50 a 75 de 88
Resumen de: CN120519328A
The invention belongs to the technical field of lead adsorption, and relates to lead-resistant lactobacillus salivarius Pb214 and application thereof. A new lead-resistant lactobacillus salivarius Pb214 is separated from cecum contents of healthy broilers, the classification name of the lead-resistant lactobacillus salivarius Pb214 is Lactobacillus salivarius, the preservation number of the lead-resistant lactobacillus salivarius Pb214 is CGMCC (China General Microbiological Culture Collection Center) NO.33992, and the lead-resistant lactobacillus salivarius Pb214 is preserved in China General Microbiological Culture Collection Center on March 27, 2025. Meanwhile, experiments prove that the maximum tolerance lead concentration of the lactobacillus salivarius is 1000mg/L, the average lead adsorption rate of the lactobacillus salivarius to a 5mg/L lead solution (pH 5) is 94.9%, the lead adsorption rate is the highest and reaches 96.33% when the pH is 7, and the lactobacillus salivarius can tolerate 0.3% cholate and also can inhibit the growth of salmonella pullorum and staphylococcus aureus. Therefore, the lead-resistant lactobacillus salivarius can be used for removing the lead metal element in a matrix, and the matrix can be soil, feed, a culture medium and other substances needing lead removal.
Resumen de: US2025261659A1
Two stage heated water treatment of shell eggs results in-shell poached eggs. The in-shell poached are sufficiently cooked so when the eggs are cracked onto a plate the eggs have the characteristics of conventional poached eggs without further cooking and need only to be warmed for consumption. The in-shell poached eggs are also pasteurized in the shell to meet the FDA and WHO requirement of a 5-log reduction of Salmonella Enteritidis.
Resumen de: WO2025171792A1
Provided is use of a combination of three or more of the following microorganisms in the preparation of an anti-tumor combination vaccine: Bordetella pertussis, Salmonella typhi, Salmonella paratyphi A, Salmonella paratyphi B, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, Proteusbacillus vulgaris, Lactic acid bacteria, Bifidobacterium longum, Bordetella pertussis, Corynebacterium diphtheriae and Clostridium tetani, Clostridium acetobutylicum, Salmonella typhimurium, and Streptococcus pyogenes. The anti-tumor combination vaccine is broad-spectrum, safe and non-toxic, and thus has the prospect of clinical applications.
Resumen de: WO2025175111A1
Two stage heated water treatment of shell eggs results in-shell poached eggs. The in-shell poached are sufficiently cooked so when the eggs are cracked onto a plate the eggs have the characteristics of conventional poached eggs without further cooking and need only to be warmed for consumption. The in-shell poached eggs are also pasteurized in the shell to meet the FDA and WHO requirement of a 5-log reduction of Salmonella Enteritidis.
Resumen de: KR20240015036A
The present invention relates to a recombinant expression vector for secretion of ifn-03b2# protein and an attenuated salmonella strain transformed by the same. provided are a recombinant expression vector comprising a flgm gene, an ifn-03b2# gene, and a flhdc gene, an attenuated salmonella strain transformed by the same, and a pharmaceutical composition for treating cancer comprising the attenuated salmonella strain as an active ingredient.
Resumen de: KR20240015036A
The present invention relates to a recombinant expression vector for secretion of ifn-03b2# protein and an attenuated salmonella strain transformed by the same. provided are a recombinant expression vector comprising a flgm gene, an ifn-03b2# gene, and a flhdc gene, an attenuated salmonella strain transformed by the same, and a pharmaceutical composition for treating cancer comprising the attenuated salmonella strain as an active ingredient.
Resumen de: CN120051482A
The present invention relates to a modified live attenuated Gram-negative bacterium wherein the bacterium has been modified such that an RNA molecule can be safely and efficiently delivered to a target eukaryotic cell. Thus, the present invention relates to a bacterial delivery system and various uses and methods thereof.
Resumen de: CN120505281A
The invention discloses a hybridoma cell strain secreting an anti-salmonella abortus groEL protein monoclonal antibody, the monoclonal antibody and application of the hybridoma cell strain. The hybridoma cell strain is named as E11, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the strain preservation number of the hybridoma cell strain is CGMCC No.46349. According to the present invention, the salmonella universal cELISA antibody detection method capable of being applied to different animals is established by using the E11 MAb secreted by the hybridoma cell strain secreting the anti-horse salmonella abortus groEL protein monoclonal antibody and the horse salmonella abortus groEL protein; the salmonella comprises salmonella abortus (S.Abortuqui), salmonella typhimurium (S.Typhi), salmonella dublin (S.Dublin) and salmonella enteritidis (S.Enteridis), so that the method disclosed by the invention has the advantages of good specificity and broad spectrum. The invention provides an effective technical means for diagnosis, prevention and control of salmonellosis of different animals clinically.
Resumen de: CN120505321A
The invention relates to the technical field of salmonella detection, in particular to a multivalent aptamer probe for capturing salmonella, a preparation method of the multivalent aptamer probe and application of the multivalent aptamer probe to salmonella detection. The invention relates to a multivalent aptamer probe for capturing salmonella. The multivalent aptamer probe is prepared from a DNA sample H1, a DNA sample H2, an activation chain, an aptamer and cDNA dry powder through a hybridization chain reaction technology. The multivalent aptamer probe is used in the salmonella detection process, and has the advantages of one-tube detection, no secondary pollution and high detection sensitivity.
Resumen de: CN120504728A
The invention discloses an application of a polypeptide in inhibiting bacteria or preparing a reagent for inhibiting bacteria, and belongs to the technical field of biology. According to preferred codons of bacillus subtilis, a polypeptide coding gene which is derived from myxobacteria and has unknown functions is designed, and a nucleotide sequence is obtained. And then carrying out recombinant expression in bacillus subtilis by utilizing a genetic engineering technology. The fermentation liquor of the recombinant bacillus subtilis shows better antibacterial activity on gram-positive bacteria represented by staphylococcus aureus, pseudomonas aeruginosa, salmonella, escherichia coli and gram-negative bacteria represented by vibrio parahaemolyticus. The antibacterial function of the polypeptide in myxobacteria is found for the first time, namely, the novel antibacterial peptide is provided, and compared with traditional antibiotics, the novel antibacterial peptide is equivalent or higher in antibacterial activity, wide in antibacterial spectrum and low in preparation cost and has wide application prospects in the fields of antibacterial preparations, food preservatives and the like.
Resumen de: CN120505438A
The invention discloses a composition and method for detecting food-borne pathogenic bacteria and application, and belongs to the technical field of food safety detection. The gDNAs and fluorescent probe combination suitable for multi-target parallel detection is obtained through multiple rounds of screening, meanwhile, the advantages of LAMP efficient amplification and PfAgo targeted cutting are combined, a multiple LAMP-PfAgo rapid detection method for three pathogenic bacteria of staphylococcus aureus, salmonella and listeria monocytogenes is established, and the problem that LAMP multiple detection is difficult is effectively solved. According to the present invention, the specificity is high, the result determination is simple, the detection limit on the pure culture can achieve 101 CFU/mL, the detection can be completed within 45 min, and the important practical application value is provided.
Resumen de: WO2025170108A1
The present invention relates to identifying and engineering a tail spike protein gene from a genome of bacteriophage SFP10 infected with a Salmonella strain, purifying and producing a recombinant protein using mSFP10TSP, which is a recombinant protein formulation for detecting Salmonella enterica, EGFP-mSFP10TSP fused with a green fluorescent protein, and SiBD-mSFP10TSP fused with an SiBD, and thereby specifically detecting the Salmonella strain.
Resumen de: CN120505437A
The invention relates to the technical field of salmonella detection, in particular to a salmonella molecular detection method and application thereof, and the salmonella molecular detection method comprises the following steps: S1, sample treatment: extracting DNA in a to-be-detected sample; step S2, primer design and synthesis: specific primers are designed for the invA gene, the hilA gene and the stn gene of salmonella; s3, multiple PCR reaction: taking the DNA extracted in the step S1 as a template, and carrying out multiple PCR amplification by adopting the primer in the step S2; s4, fluorescent probe hybridization: hybridizing a PCR amplification product in the step S3 with a fluorescent labeled probe; s5, fluorescence signal detection: detecting a fluorescence signal after hybridization through a fluorescence detector, and judging whether the sample contains salmonella or not according to the signal. According to the salmonella molecular detection method provided by the invention, a technology of combining multiple PCR with fluorescent probe hybridization is adopted, a plurality of conserved genes of salmonella can be detected at the same time, and the detection specificity and sensitivity are improved.
Resumen de: CN120478554A
The invention relates to the technical field of animal medicines, and particularly discloses a veterinary medicine compound preparation as well as a preparation method and application thereof. The veterinary medicine compound preparation is prepared by boiling, extracting, concentrating and diluting eight Tibetan medicines including aconitum pendulum, fructus chebulae, radix inulae inula, benzoin, pterocephalus hookeri, lagotis lagotis, amomum tsao-ko and rhodiola rosea. The veterinary drug compound preparation provided by the invention has an antibacterial range of 8.67 mm to 23.77 mm on enterotoxic large intestine dried bacteria, salmonella enteritidis and pasteurella multocida, and has an inhibition rate of 60% to 72% on enterotoxigenic escherichia coli diarrhea, salmonella enteritidis diarrhea and pasteurella multocida diarrhea; therefore, the veterinary drug compound preparation provided by the invention has a remarkable inhibition effect on enterotoxic large intestine dry bacteria, salmonella enteritidis and pasteurella multocida.
Resumen de: CN120484082A
The invention relates to the technical field of biology, in particular to an antibacterial peptide Aquiluscidin-1, a coding gene and application of the antibacterial peptide Aquiluscidin-1. The invention provides an antibacterial peptide Aquiluscidin-1 which has good thermal stability, acid-base stability and endogenous protease degradation resistance, can obviously inhibit the growth of escherichia coli, salmonella and staphylococcus aureus, and has good application prospects in the fields of food, hygienic products, cosmetics, biopesticide, biological feed additives or natural food preservatives and the like. Wide application prospects are realized.
Resumen de: CN120484134A
The invention relates to the technical field of biology, in particular to hermetia illucens antibacterial peptide H3 fusion protein, a construction method and application. The novel hermetia illucens antibacterial peptide H3 fusion protein provided by the invention not only has the antibacterial activity of the antibacterial peptide, but also has the activity of glucoside hydrolase, can realize rapid visual monitoring of the activity of the antibacterial peptide, has an inhibition effect on escherichia coli, salmonella and staphylococcus aureus, and can be used for preparing the antibacterial peptide H3 fusion protein. In the fields of food, hygienic products, cosmetics, biopesticides, biological feed additives or natural food preservatives and the like, the method has a wide application prospect.
Resumen de: CN120478388A
The invention discloses antibacterial application of mulberry polysaccharide, and the mulberry polysaccharide is prepared by the following method: drying fresh mulberry, crushing, and adding into absolute ethyl alcohol for extraction; then taking the precipitate and drying; adding into distilled water, performing water bath, centrifuging, taking supernate, performing vacuum concentration, centrifuging again, taking precipitate, adding distilled water into the precipitate, redissolving, and drying to obtain mulberry polysaccharide; the mulberry polysaccharide obtained by the method has a relatively good inhibition effect on gram-negative bacteria escherichia coli and salmonella and gram-positive bacteria staphylococcus aureus; the antibacterial effect is enhanced along with the rise of the temperature, so that the heat stability is achieved, and meanwhile, the good antibacterial effect is achieved in both acid and alkali environments; the mulberry polysaccharide can be further prepared into a novel natural bacteriostatic agent and has a wide application prospect in the fields of daily chemicals and medicines.
Resumen de: CN120484133A
本发明提供了一种基于猪圆环病毒2型(PCV2)纳米抗体与沙门氏菌鞭毛蛋白的重组融合蛋白及其制备方法与应用。本发明将PCV2衣壳蛋白(Cap)特异性纳米抗体基因与沙门氏菌鞭毛蛋白基因融合，使用大肠杆菌原核表达系统表达制备了Nbcap‑flagellin重组融合蛋白。该重组融合蛋白能够利用Cap蛋白特异性纳米抗体的靶向结合能力，实现鞭毛蛋白佐剂与抗原蛋白的动态偶联，形成佐剂用量可调控的抗原‑佐剂复合物；在充分发挥鞭毛蛋白作为免疫佐剂作用的同时，突破传统鞭毛蛋白与抗原蛋白融合表达导致的固定佐剂‑抗原配比限制，提升了疫苗设计的灵活性，避免了细菌鞭毛蛋白过量可能引发的炎性反应，为开发高效、安全且广谱的PCV2亚单位疫苗提供了新的技术路径。
Resumen de: CN120485133A
The invention discloses a salmonella bacteriophage Y1 with a wide lysis spectrum and a preparation method of a low-endotoxin preparation of the salmonella bacteriophage Y1. The preservation number of the salmonella bacteriophage Y1 is CCTCC M 20242911. The method comprises the following steps: adding a chitosan solution into a bacteriophage suspension, uniformly mixing, and precipitating overnight at room temperature. The salmonella phage Y1 disclosed by the invention is high in cracking capacity and can be applied to preparation of drugs for preventing and treating salmonella. The method disclosed by the invention is low in cost, simple in used equipment, simple and convenient in operation procedure, stable in effect, easy for large-scale industrial production, low in endotoxin preparation 3, good in endotoxin removal effect and small in influence on the activity of the bacteriophage.
Resumen de: WO2025170108A1
The present invention relates to identifying and engineering a tail spike protein gene from a genome of bacteriophage SFP10 infected with a Salmonella strain, purifying and producing a recombinant protein using mSFP10TSP, which is a recombinant protein formulation for detecting Salmonella enterica, EGFP-mSFP10TSP fused with a green fluorescent protein, and SiBD-mSFP10TSP fused with an SiBD, and thereby specifically detecting the Salmonella strain.
Resumen de: WO2025170306A1
The present invention relates to an antibacterial composition comprising, as an active ingredient, a plantaricin peptide derived from a Lactiplantibacillus plantarum KM2 strain, wherein the plantaricin peptide derived from the strain and a plantaricin peptide combination were found to have antimicrobial activity against at least one selected from the group consisting of Flavobacterium sp, Enterococcus faecalis, Listeria monocytogenes, Staphylococcus aureus, and acromobacter xylosoxidans (Alcaligenese xylosoxidans), Salmonella enterica, <i />and Vibrio parahaemolyticus, and the plantaricin peptide and the plantaricin peptide combination were found to have antibacterial activity and a cell wall lysis effect against the Flavobacterium sp. strain, and accordingly, the plantaricin peptide derived from the Lactiplantibacillus plantarum KM2 strain and the plantaricin peptide combination are provided as antimicrobial agents.
Resumen de: KR20250122779A
구현예의 수의학적 조성물은 가금류의 대장균 또는 살모넬라균에 의한 설사 증상의 치료 또는 완화용도를 갖는 것으로, 색도 L이 77 이하인 액상이고, 유효성분 및 항산화제를 포함하고, 상기 유효성분은 아프라마이신 또는 이의 염을 유효성분을 포함한다. 구현예의 수의학적 조성물은 조류의 대장균증 또는 추백리의 치료 또는 완화용으로 그 효과가 우수하다. 또, 구현예의 수의학적 조성물은 아프라마이신 또는 이의 염을 유효성분으로 포함하는 액상 조성물로 유효성분에 대한 조성물의 보관 안정성이 우수하고, 투여가 용이한 장점도 있다.
Resumen de: MY209939A
The present invention relates to a molecular detection kit for simultaneously detecting hemorrhagic bacteria (100), comprising a PCR reaction mastermix; a primer pool that comprises a plurality of specific primer pairs of bacteria that include forward and reverse primers and an internal control; and a buffer solution; characterized in that, the internal control is of Vibrio cholera hemA gene; and a plurality of specific primers of bacteria further comprising Shigella spp. 2a plasmid pMYSH6000 (virA) gene; Campylobacter 16S ribosomal RNA (rRNA) gene; Salmonella spp. outer membrane porin C (ompC) gene; and E.coli E6-3 intimin (eaeA) gene. The method for detecting the bacteria consisting of Shigella, Campylobacter, Salmonella and E. coli (200) using the molecular detection kit (100) is also provided. Figure 1
Resumen de: CN120468246A
The invention provides a magnetic control type electrochemical biosensor of MWCNTs-MoS2 based on CRISPR (clustered regularly interspaced short palindromic repeats)/Cas12a as well as a preparation method and application of the magnetic control type electrochemical biosensor. The magnetic control type electrochemical biosensor comprises an aminated multi-walled carbon nanotube-molybdenum disulfide modified screen-printed carbon electrode, wherein a CRISPR-crRNA-Target DNA ternary complex and a ferroferric oxide-gold-single-stranded nucleotide probe are dropwise added on the surface of the screen-printed carbon electrode, and the probe is sheared after the reaction of the CRISPR-crRNA-Target DNA ternary complex and the ferroferric oxide-gold-single-stranded nucleotide probe; the magnetic control type electrochemical biosensor respectively shows wide linear response in the range of 4.6 * 10 < 1 >-4.6 * 10 < 7 > CFU/mL of staphylococcus aureus and 5.5 * 10 < 7 > CFU/mL of salmonella, the detection limit of the magnetic control type electrochemical biosensor to two pathogenic bacteria is as low as 2CFU/mL, and the magnetic control type electrochemical biosensor has good specificity to interfering bacteria, can be used for rapid detection of food safety and has good practicability.
Nº publicación: CN120464525A 12/08/2025
Solicitante:
HENAN JINBAIHE BIOTECHNOLOGY CO LTD
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Resumen de: CN120464525A
The invention discloses a bacillus cereus JBH-LY1 strain and an application of the bacillus cereus JBH-LY1 strain in aquaculture, and relates to the technical field of microorganisms. The bacillus cereus JBH-LY1 is preserved in the China General Microbiological Culture Collection Center on June 6, 2024, and the preservation number of the bacillus cereus JBH-LY1 is CCTCC (China Center for Type Culture Collection) NO: M 20241168. The bacillus cereus is natural, safe, acid-resistant, alkali-resistant and high-temperature-resistant, has strong capability of producing protease, amylase, lipase and cellulase, and has a bacteriostatic effect on escherichia coli, salmonella typhimurium, staphylococcus aureus and aeromonas hydrophila. The bacillus cereus is used in the aquaculture industry, can purify aquaculture water and reduce the content of nitrogen and phosphorus, and also can regulate intestinal flora balance of cultured animals, promote growth, improve disease resistance and increase culture benefits.
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