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口服疫苗

Resumen de: WO2024180083A1

The present invention relates to a non-viable bacterium cell wherein an immunogenic polypeptide fused to an autotransporter comprising transmembrane linker and a trans- porter domain is displayed on the surface of the cell, and to a preparation comprising such non-viable cells. The preparation is useful as an oral vaccine.

BACTERIA AS A CARRIER TO DELIVER NUCLEIC ACID VECTORS

Resumen de: WO2025189290A1

The present invention relates to the use of bacteria, such as Salmonella, as a carrier for an expression vector that encodes all or a portion of replicon proteins from a positive stranded virus, wherein expression of the replicon proteins is under the control of eukaryotic and/or prokaryotic promoters, and wherein expression of a payload is under the control of a sub-genomic promoter. Also provided are methods of using the bacteria comprising the vector in therapeutics and vaccines.

ANTI-BACTERIAL PROTEIN COMPLEX

Resumen de: AU2024217948A1

The invention relates to protein bacteriocins (PBs) as therapeutic agents, and specifically to protein complexes comprising two or more PB molecules associated with a protein scaffold which comprises cognate immunity protein domains for the effector portions of the respective PBs. In particular, the invention provides an anti-bacterial protein complex comprising (a) a first PB molecule and a second PB molecule; and (b) an immunity protein scaffold comprising a first immunity protein domain and a second immunity protein domain; wherein the first and second immunity protein domains are non-covalently bound to the respective first and second PB molecules.

一种含磷污水的处理方法

Resumen de: CN120622690A

本发明涉及生物技术处理污水的技术领域，尤其涉及一种含磷污水的处理方法。所述方法包括以下步骤：1）调节含磷污水的pH值为7.0‑7.5，补充乙酸钠和铵盐至浓度分别为0.1‑0.2g/L和0.01‑0.04g/L；2）在上述污水中接种聚磷菌，接种浓度为2‑5%；3）对污水中的磷污染物进行检测，符合排污标准后，排出污水。本发明对微生物进行改造，显著提高了菌体体内多聚磷酸物的水平，其改造相较于野生型提升了87.6%；导入RcsA基因后对于其荚膜产量有明显提升，而且将重度磷污染的污水中磷的含量降低到8.4mg/L，为其在商业中使用奠定了基础。

一种猪源细菌多糖-病毒蛋白结合疫苗及其制备方法和应用

Resumen de: CN120605324A

本发明涉及一种猪源细菌多糖‑病毒蛋白结合疫苗及其制备方法和应用，属于动物疫苗技术领域。该疫苗以猪源细菌多糖和病毒蛋白为原料，通过生物素‑亲和素体系偶联形成多糖‑病毒蛋白结合物；其中病毒蛋白选自猪瘟病毒E2蛋白、猪圆环病毒2型Cap蛋白、猪伪狂犬病毒gD蛋白等蛋白中的一种，猪源细菌多糖选自猪链球菌荚膜多糖、猪多杀性巴氏杆菌荚膜多糖等多糖中的一种。该疫苗能同时诱导机体产生针对猪源细菌荚膜多糖和病毒蛋白的高水平IgG抗体，实现对猪源细菌和病毒感染的协同免疫保护，具有广谱交叉免疫效果。

-7 Recombinant Expression Vector for Secretion of Interleukin-7 and Attenuated Salmonella Transformed Therewith

Resumen de: KR20240015036A

The present invention relates to a recombinant expression vector for secretion of ifn-03b2# protein and an attenuated salmonella strain transformed by the same. provided are a recombinant expression vector comprising a flgm gene, an ifn-03b2# gene, and a flhdc gene, an attenuated salmonella strain transformed by the same, and a pharmaceutical composition for treating cancer comprising the attenuated salmonella strain as an active ingredient.

VACCINE COMPOSITION COMPRISING A SYSTEM FOR DELIVERING AN INACTIVATED WHOLE BACTERIUM VIA CATIONIC POLYSACCHARIDE NANOPARTICLES WITHOUT ANY ADJUVANT

Resumen de: AU2024220779A1

The invention relates to the field of vaccine compositions. The invention more particularly relates to a prophylactic vaccine composition that is intended for mammals and birds and comprises a killed whole bacterium, said bacterium being covered with a cationic agent, in particular cationic nanoparticles.

vB_ESM-pEJ01 Lytic bacteriophage vB_ESM-pEJ01 that simultaneously infects E. coli and Salmonella bacteria and is effective in removing biofilm and controlling pathogen-contaminated food and its uses

Resumen de: KR20250131847A

본 발명은 대장균 및 살모넬라 균을 동시에 감염하며, 생물막 제거와 병원체 오염 식품 제어에 효과적인 용균성 박테리오파지 vB_ESM-pEJ01에 관한 것으로, 본발명에 따른 파지 및/또는 조성물은 시가 독소 생성 대장균을 제거하기 위한 항균제로 이용될 수 있다.

REACTION MEDIUM AND ASSOCIATED METHOD FOR DETECTION OF A TARGET MICROORGANISM

Resumen de: US2025277250A1

The invention relates to a gelled reaction medium for the detection, identification, enumeration and/or isolation of at least one target microorganism in a simple that may contain same, comprising at least one binding partner specific to a component of a target microorganism or of a component derived from said microorganism, coupled to at least one nanoparticle to form at least one binding conjugate.

VACCINE COMPOSITION COMPRISING A SYSTEM FOR DELIVERING AN INACTIVATED WHOLE BACTERIUM VIA CATIONIC POLYSACCHARIDE NANOPARTICLES WITHOUT ANY ADJUVANT

Resumen de: MX2025009500A

The invention relates to the field of vaccine compositions. The invention more particularly relates to a prophylactic vaccine composition that is intended for mammals and birds and comprises a killed whole bacterium, said bacterium being covered with a cationic agent, in particular cationic nanoparticles.

Microbial treatment flora for treating odor of household garbage treatment station

Resumen de: CN120555263A

The invention discloses a microbiological treatment flora for treating odor of a household garbage treatment station. Comprising the following microbial treatment flora components: 12 to 33 percent of biphasic photosynthetic bacteria, 0.2 to 1.5 percent of clostridium butyricum, 8 to 22 percent of bifidobacterium, 0.2 to 1.5 percent of nitrogen-fixing bacteria, 9 to 18 percent of mesophilic actinomycetes, 0.3 to 2 percent of potassium bacteria, 13 to 23 percent of subdigestion bacteria, 0.3 to 2 percent of phosphate solubilizing bacteria, 18 to 24 percent of bacillus megatherium, 0.5 to 3 percent of lactic acid bacteria and 0.5 to 3 percent of saccharomycetes. Fatty acids and sulfides are decomposed through the biphase photosynthetic bacteria through photosynthesis, stink precursor substances are reduced from the source, bifidobacteria and lactic acid bacteria reduce the pH of the environment through acid production, growth of stink-producing putrefying bacteria such as escherichia coli and salmonella is inhibited, release of stink gases such as ammonia and methyl mercaptan is reduced, saccharomycetes generate alcohol through saccharides, and the alcohol content is reduced. The removal rate of hydrogen sulfide and ammonia is greater than or equal to 80%, and the degradation rate of the volatile fatty acid is greater than or equal to 70%.

Escherichia coli bacteriophage and expression and application of endolysin thereof

Resumen de: CN120555369A

The invention discloses expression and application of an Escherichia coli phage and endolysin thereof, and belongs to the technical field of microorganisms, the Escherichia coli phage VB-EcoM-WM is separated from diarrhea cattle manure of a certain cattle farm in Sichuan, the Escherichia coli phage VB-EcoM-WM is preserved in China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 2025810, the endolysin of the Escherichia coli phage VB-EcoM-WM has broad-spectrum lysing property on bacteria, and the endolysin of the Escherichia coli phage VB-EcoM-WM has broad-spectrum lysing property on bacteria. The endolysin is subjected to hydrophobic modification and prokaryotic expression to obtain the fused endolysin protein, so that the cleavage property of the endolysin is more broad-spectrum and efficient. The gene engineering modified fusion protein not only can crack host escherichia coli, but also has a bacteriostatic effect on 15 tested bacteria such as salmonella, streptococcus, staphylococcus and pasteurella. The minimum inhibitory concentration on escherichia coli is 0.1 mu g/ml. The traditional antibiotics can be replaced.

Lactobacillus plantarum RT1 with anti-inflammatory function and application thereof

Resumen de: CN120555287A

The invention discloses a lactobacillus plantarum RT1 with an anti-inflammatory effect and application of the lactobacillus plantarum RT1. The lactobacillus plantarum RT1 with the anti-inflammatory effect is screened by utilizing a J774-Dual macrophage inflammation model, the influence of the RT1 on the cell activity is evaluated, and the anti-inflammatory effect of the lactobacillus plantarum RT1 is researched. It is found that the lactobacillus plantarum RT1 significantly inhibits LPS and salmonella induced NF-kappa B activation, inhibits expression of pro-inflammatory cytokines IL-1beta, TNF-alpha and IL-6, meanwhile up-regulates the expression level of an anti-inflammatory cytokine IL-10, and maintains good cell activity; dSS-induced weight loss, occult blood, colon shortening and DAI score are significantly relieved, the MPO level and inflammatory cytokine expression are reduced, and colon tissue pathological damage is improved. The lactobacillus plantarum RT1 has the function of inhibiting intestinal inflammation, so that a new material is provided for the development of novel efficient anti-inflammatory probiotics.

一种基于细菌外膜囊泡的口服疫苗的制备方法及其应用

Resumen de: CN120550103A

一种基于细菌外膜囊泡的口服疫苗制备方法及其应用，可有效应用于制备预防或治疗肠道黏膜病原体感染的药物，克服传统注射型疫苗依从性差、黏膜免疫反应低的问题。首先制备粪产碱杆菌的细菌外膜囊泡，然后制备DSPE‑PEG‑antigen，将两者混合后搅拌，制得OMV‑DSPE‑PEG‑antigen，取菊粉溶于PBS中，加热振荡，冷却至室温，加入OMV‑DSPE‑PEG‑antigen，搅拌，放置，得成品口服疫苗。制备工艺简单，方法稳定可靠，易操作，生产成本低，制备的基于细菌外膜囊的口服疫苗可有效激发肠道黏膜免疫应答，预防黏膜病原体的感染，经济和社会效益显著。

Arachidonic acid ethanolamine composition and application thereof

Resumen de: CN120569193A

The composition containing the arachidonic acid ethanolamine or the derivatives of the arachidonic acid ethanolamine is used for preparing nutritional supplements, health-care products, foods and beverages for regulating intestinal flora, the level of a first intestinal microbiota in a subject can be remarkably improved, meanwhile, the level of a second intestinal microbiota in the subject is reduced, and then intestinal health is improved. The first intestinal microbiota is selected from one or more of the following groups: Rosella, Bacteroides and Prevotella; the second intestinal microbiota is salmonella or escherichia coli.

Salmonella drug resistance monitoring data safety management system based on block chain

Resumen de: CN120567452A

The invention provides a salmonella drug resistance monitoring data security management system based on a block chain, and belongs to the technical field of data transmission management. A hierarchical encryption mechanism is constructed, the technical characteristics of the block chain are fused, a standardized feature combination template architecture is designed, and a matched hierarchical transmission and intelligent contract verification system is used; a differential encryption strategy is implemented on the basis of data sensitivity, and the security of each level of data is guaranteed in combination with symmetric and asymmetric encryption technologies; through characteristic parameter modularization recombination and template association identification, data integrity is guaranteed, transmission efficiency is optimized at the same time, and scattered data can be precisely restored into a structured data set; and finally, through consensus node verification driven by an intelligent contract and on-chain state updating, whole-process standardized management and control of data from collection, transmission to storage are realized, and a salmonella drug resistance monitoring data security management mode with security, efficiency and traceability is constructed.

Lateral flow chromatography kit for high-sensitivity quantitative detection of salmonella enteritidis

Resumen de: CN120539398A

The invention belongs to the technical field of food microorganism detection, and provides a lateral flow chromatography kit for high-sensitivity quantitative detection of salmonella enteritidis, which comprises a salmonella enteritidis RPA primer group and a lateral flow chromatography test strip, raman label streptavidin, a Digoxin antibody and a streptavidin antibody are coated on the lateral flow chromatography test strip. The reporter probe is combined on the release pad of the flow measurement chromatography test strip, has two characteristics of colorimetry and SERS (Surface Enhanced Raman Scattering), can finish simultaneous qualitative and quantitative detection of pathogenic bacteria, and improves the detection sensitivity; and the Digoxin antibody and the streptavidin antibody are respectively combined on the detection line and the quality control line. The kit does not need to use large experimental equipment in the whole detection process, is high in detection accuracy and good in specificity, and is very suitable for real-time detection under the condition of relatively low development level.

Biological bacteriostatic agent and preparation method thereof

Resumen de: CN120536277A

The invention discloses a biological bacteriostatic agent and a preparation method thereof, and the preparation method comprises the following steps: step one to step three, inoculating lactic acid bacteria into a liquid fermentation culture medium, and carrying out three-stage fermentation culture; step 4, after the third-stage seed fermentation is finished, performing high-speed centrifugation on the fermentation liquid to obtain a bacterial sludge compound; 5, uniformly mixing the bacterial sludge compound with a protective agent, and performing freeze drying to obtain microbial freeze-dried powder; and 6, uniformly mixing the microbial freeze-dried powder, the auxiliary agent and the auxiliary factor to obtain the biological bacteriostatic agent. The biological bacteriostatic agent prepared by the method disclosed by the invention has remarkable bacteriostatic and bactericidal effects on various pathogenic bacteria, such as escherichia coli, salmonella and staphylococcus aureus; and main antibacterial substances come from lactic acid bacteria and metabolites thereof, so that pathogenic bacteria do not generate drug resistance to the antibacterial substances. The natural components are adopted for fermentation preparation, no chemical component is contained, the environment-friendly effect is achieved, and safety is high; and the preparation method is simple and easy for industrial production.

Antibacterial peptide Meijia-1 and application thereof

Resumen de: CN120518710A

The invention belongs to the technical field of biology, and particularly discloses an antibacterial peptide Meijia-1 and application thereof. The amino acid sequence of the antibacterial peptide is as follows: arginine, glycine, arginine, phenylalanine, glycine, arginine, phenylalanine, leucine, lysine, lysine, valine, arginine, arginine, phenylalanine, isoleucine, proline, lysine and valine. The antibacterial peptide Meijia-1 has better antibacterial activity on gram-positive bacteria (staphylococcus aureus) and gram-negative bacteria (escherichia coli and salmonella), and has the characteristics of heat resistance, salt resistance, acid and alkali resistance, repeated freezing and thawing resistance, serum resistance, organic solvent resistance and pepsin resistance. Therefore, the antibacterial peptide Meijia-1 has wide application prospects in the fields of pathogenic infection resistance, animal feed, food preservation and preservatives, cosmetics, detergents, disinfectants, pesticides, antibacterial molecular materials and the like.

Nano-enzyme composite material for detecting pathogenic microorganisms in complex matrix and application of nano-enzyme composite material

Resumen de: CN120522382A

The invention discloses a nano-enzyme composite material for detecting pathogenic microorganisms in a complex matrix and application of the nano-enzyme composite material, and belongs to the technical field of pathogen detection. The kit containing the nano-enzyme composite material provided by the invention can realize accurate detection of food-borne pathogenic bacteria in a complex matrix, and has an excellent visual detection effect. And the environment-stable storage performance is excellent and can be up to 60 days. Excellent catalytic activity, stability and selectivity are shown in the aspect of detecting escherichia coli, staphylococcus aureus, vibrio parahaemolyticus and salmonella typhimurium. According to the invention, rapid and reliable detection can be realized, and the detection limit of different pathogens in a PB buffer solution (pH 7.2) is 2-7CFU/mL.

Kit for detecting shigella by Marker method and detection method

Resumen de: CN120522381A

The invention provides a kit for detecting shigella by a Marker method, and the Marker method represents fluorescence intensity corresponding to shigella with different concentrations and is a basis for establishing a Marker graph; the kit comprises a Marker graph, a magnetic aptamer, a detection aptamer, a bacterial lysis solution, an enzyme digestion solution, a binding solution, a washing solution and a magnetic frame. The detection method comprises the following steps: the kit is used for splitting the shigella through a bacterial lysis solution to release DNA, the DNA is cut through an enzyme digestion solution to obtain a fragment rich in an ipaH gene, the magnetic aptamer and the detection aptamer are specifically combined with the ipaH fragment under the action of a binding solution to form a compound, the compound is washed with washing liquid, the fluorescence intensity is detected and compared with a Marker diagram, and quantitative detection of the shigella is realized. The method does not need to treat a sample in advance, shortens the detection time, and has strong specificity and high sensitivity.

Application of synanthrin in improving action efficiency of ampicillin on drug-resistant salmonella

Resumen de: CN120514728A

The invention discloses application of synanthrin in improving the action efficiency of ampicillin on drug-resistant salmonella, and relates to the technical field of medical biology. Researches show that synanthrin does not have an inhibiting effect on salmonella, but synanthrin and ampicillin are used together, the inhibiting effect of ampicillin on salmonella can be remarkably improved, and same conclusions are drawn by in-vitro tests and in-vivo tests. According to the invention, the use of antibiotics can be reduced, and a new research direction is provided for the prevention and treatment of salmonella.

Fusion protein H3-OtTx1a, construction method and application

Resumen de: CN120518775A

The invention relates to the technical field of biology, in particular to fusion protein, a construction method and application. A new fusion gene is constructed by adopting an SOE-PCR technology for the first time, the fusion protein H3-OtTx1a is obtained by expression in a suitable expression system, the functions of parent proteins of the fusion protein H3-OtTx1a are integrated, the antibacterial activity of the fusion protein H3-OtTx1a is higher than that of hermetia illucens antibacterial peptide H3 and spider peptide OtTx1a, and the fusion protein H3-OtTx1a has good thermal stability, acid-base stability and endogenous protease degradation resistance, and can be used for preparing the antibacterial peptide H3 and spider peptide OtTx1a. Growth of enterobacter, salmonella and staphylococcus aureus can be obviously inhibited, and the compound has wide application prospects in the fields of food, hygienic products, cosmetics, biopesticides, biological feed additives or natural food preservatives and the like.

Application of tea polyphenol in yellow tea in bacterium inhibition

Resumen de: CN120514781A

The invention relates to the technical field of application of natural active substances, in particular to application of tea polyphenol in yellow tea in inhibition of bacteria, gram-positive bacteria comprise staphylococcus aureus, bacillus subtilis, enterococcus faecalis and enterococcus faecium. The gram-negative bacteria comprise escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, proteus mirabilis, salmonella and acinetobacter baumannii. The bacteriostatic activity and related mechanism of the tea polyphenol in the yellow tea are systematically studied, the bacteriostatic effect of the tea polyphenol in the yellow tea on gram-negative bacteria and gram-negative bacteria is proved, and the bacteriostatic mechanism of the tea polyphenol in the yellow tea on bacteria is disclosed through bacteriostatic experiments of the tea polyphenol on different bacteria, so that the bacteriostatic effect of the tea polyphenol in the yellow tea on the gram-negative bacteria and the bacteriostatic effect of the tea polyphenol in the yellow tea on the gram-negative bacteria are proved. The bacteriostatic activity and related mechanisms of the tea polyphenol in the yellow tea are systematically researched, the development of potential clinical medicinal value and edible value of the tea polyphenol is facilitated, and a foundation is laid for the application of the tea polyphenol in the fields of food, medicine and the like.

表达FLIC或FLIC-HIL2的减毒禽沙门氏菌及其用途

Nº publicación: CN120530123A 22/08/2025

Solicitante:

奥德修斯生物株式会社

Resumen de: WO2024155027A1

The present invention relates to an attenuated Salmonella gallinarum expressing FliC or FliC-hiL2 and use thereof. The Salmonella strain according to the present invention has excellent immune activity and exhibits excellent anti-cancer efficacy, and thus can be used as a therapeutic agent for cancer together with or independently of existing anti-cancer drugs.