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Primer combination for multiple detection kit of food-borne pathogenic bacteria and multiple detection kit

Resumen de: CN120400381A

The invention provides a primer combination of a multiple detection kit for food-borne pathogenic bacteria and the multiple detection kit. The primer combination comprises probes and primers for detecting nine food-borne pathogenic bacteria: vibrio cholerae general type, vibrio cholerae O1 type, vibrio cholerae O139 type, vibrio parahaemolyticus, salmonella, yersinia enterocolitica, campylobacter, listeria monocytogenes and enterobacter sakakaakii, and the sequence is shown as SEQ1-31; the probe and the primer pair are used for detecting nine characteristic genes, namely a shigella universal type: ipaH, a diarrheogenic Escherichia coli universal type: eltA, sta3, sta and aggR, and enterohemorrhagic Escherichia coli: stx1, stx2, escV and rfbE, and the sequences of the probe and the primer pair are shown as SEQ32-64. According to the invention, by combining a digital PCR multiple coding technology, the detection of 12 food-borne pathogenic bacteria can be realized, and the sensitivity, the specificity and the accuracy are relatively good.

Application of bilobalide in preparation of product for resisting salmonella typhimurium infection

Resumen de: CN120392738A

The invention discloses an application of bilobalide in preparation of a product for resisting salmonella typhimurium infection. The bilobalide takes salmonella typhimurium QseB protein as a target, has a good interference effect on an AI-2 quorum sensing signal of salmonella typhimurium and has good pharmacological activity, and particularly, the bilobalide has good pharmacological activity under the condition that the growth of salmonella typhimurium is not inhibited. The motility, biofilm formation and infection pathogenicity of salmonella typhimurium are obviously inhibited. The bilobalide does not influence the growth of salmonella typhimurium, directly acts on pathogenic factors, weakens the pathogenicity of bacteria from the source, does not generate selective pressure on salmonella typhimurium, and does not cause the generation of drug-resistant pathogenic bacteria. Therefore, the bilobalide has a good application prospect in the aspect of development of the salmonella typhimurium infection resisting medicine.

Composite nano-material probe for detecting salmonella typhimurium, preparation method of composite nano-material probe and dual-mode immunochromatography test strip

Resumen de: CN120405121A

The invention belongs to the technical field of biochemical analysis and detection, and particularly relates to a composite nanomaterial probe for detecting salmonella typhimurium, a preparation method of the composite nanomaterial probe and a dual-mode immunochromatography test strip. According to the present invention, the improved dual-mode reading is achieved by improving the signal label, the BrM (at) Os with the surface modified with the rabbit anti-salmonella typhimurium polyclonal antibody is adopted as the detection probe, and the fluorescence and catalytic colorimetric dual-mode immunochromatography test strip capable of detecting salmonella typhimurium is prepared; the immunochromatographic test strip can realize sensitive immunochromatographic analysis on salmonella typhimurium in both fluorescence and catalytic colorimetric modes.

Double-target primer probe composition, application and kit

Resumen de: CN120400375A

The invention discloses a double-target primer probe composition, application and a kit. The double-target primer probe composition comprises amplification primers and fluorescent probes of a salmonella invasion related gene InvA and an avian pathogenic escherichia coli aerobacin receptor gene IutA. The composition can be used for detecting pathogenic bacteria in poultry farms, and is high in sensitivity, good in specificity, reliable in result and free of cross interference among primers; the kit is suitable for a recombinase polymerase amplification technology, can be used for clinical diagnosis and epidemic condition monitoring of avian pathogenic escherichia coli and salmonella, and has excellent industrial application value.

SALMONELLA STRAIN WITH CHROMOSOMALLY INTEGRATED LANDING PAD

Resumen de: CN119923470A

The present invention relates to a modified live attenuated Salmonella strain comprising at least one chromosomal integrated synthetic polynucleotide sequence inserted in a predetermined pseudogenomic position, said sequence comprising at least one defined recombination site for introducing a heterologous polynucleotide sequence encoding a polypeptide, wherein the chromosome integration synthetic polynucleotide sequence is located within at least one genomic site, the genomic site being defined by any one of SEQ ID NO: 1-30 or a sequence having at least 70% identity with any one of SEQ ID NO: 1-30. The invention also relates to vaccine compositions comprising the modified strains and various uses and methods thereof.

Lactobacillus farciminis SR2 and use thereof

Resumen de: GB2637451A

Disclosed are a lactobacillus farciminis SR2 and a use thereof. The present lactobacillus farciminis SR2 shows good tolerance under an acidic condition of pH = 3.0, has strong adaptability in 45 C° high-temperature and high salt (10% NaCl) environment, inhibits the growth of harmful bacteria such as escherichia coli, staphylococcus aureus, and salmonella, has high productivity of ferulic acid esterase, can significantly lower the pH level of rice straw silage feed, significantly increase the content of lactic acid, significantly reduce the content of ammonia nitrogen, significantly increase the content of dry matter and WSC, significantly reduce the content of NDF, ADF and cellulose, improve the fermentation quality of the rice straw silage feed, and the method can be widely applied to the field of rice straw fermentation feed preparation.

Broad-spectrum salmonella bacteriophage SD40-16 and application thereof

Resumen de: CN120381470A

The invention belongs to the technical field of microbial sterilization preparations, and particularly discloses a broad-spectrum salmonella bacteriophage SD40-16 and application of the broad-spectrum salmonella bacteriophage SD40-16. The bacteriophage SD40-16 can be used for cracking six serotypes of salmonella and escherichia coli including salmonella enteritidis and salmonella typhimurium, is wide in host range and strong in killing capability, and can be used for inhibiting the growth of bacteria. And meanwhile, the strain has good thermal stability and acid-base tolerance, and is easy to proliferate and enrich. The bacteriophage can enhance the antibacterial ability of kanamycin, provides a new drug combination strategy, has an obvious synergistic effect on salmonella resistance when being combined with kanamycin, and has a good application prospect in the aspect of preventing and treating salmonella infection.

Method and kit for rapidly testing sensitivity of salmonella to ceftriaxone

Resumen de: CN120384115A

The invention discloses a method for rapidly testing sensitivity of salmonella to ceftriaxone. The method comprises the following steps: S1, preparing a salmonella solution; s2, centrifuging the bacterial liquid and removing supernate of the centrifuged bacterial liquid; s3, providing a ceftriaxone solution, wherein the ceftriaxone solution contains ceftriaxone and water; s4, mixing the ceftriaxone solution with the bacterial solution to form a mixed solution, placing the mixed solution in an incubator, and standing for a first preset time; s5, centrifuging the mixed solution after standing for a first preset time, and extracting supernate of the mixed solution; and S6, identifying the ceftriaxone in the supernatant of the mixed solution by using a time-flight mass spectrometer, and judging the sensitivity of the salmonella to the ceftriaxone according to whether a characteristic peak corresponding to the ceftriaxone disappears or not. Compared with PCR (polymerase chain reaction) and LAMP (loop-mediated isothermal amplification) methods, the method has the advantages that the detection result is more reliable, the time consumed by the method is less than that consumed by manual drug sensitivity and full-automatic drug sensitivity identification instruments, and the method can be used for conventional detection in laboratories to timely feed back drug sensitivity results to clinics.

Application of combination of ebbeselenium and polymyxin in preparation of salmonella infection resisting medicine

Resumen de: CN120381506A

The invention belongs to the technical field of biological medicine, and discloses application of ebb-selenium as a polymyxin synergist in inhibition of salmonella. According to the application, the combination of ebb selenium and polymyxin has a good synergistic effect on salmonella in vitro and in vivo experiments. In a mouse salmonella infection model, compared with the bacterium carrying amount after single-drug treatment, the bacterium carrying amount in the animal liver after EBS and polymyxin combined treatment has the effect that the bacterium carrying amount in the animal liver is obviously reduced. The survival rate of animals treated by the combination of the EBS and the polymyxin is obviously increased compared with the survival rate of the animals treated by single medicines of the EBS and the polymyxin. Compared with the traditional method for killing salmonella through combination of antibiotics, the method has the advantages that the generation of bacterial drug resistance is not easily induced through the synergistic sterilization of the ebb-selenium and the polymyxin, and the ebb-selenium has the characteristics of wide source, multiple effects and good treatment effect, and has better research and application significance for excavating the synergistic replacement of the antibiotics and solving the bacterial drug resistance.

Pharmaceutical composition or vaccine for treating and/or inhibiting salmonella infection and application of small molecule compound Epetrabole hydrochloride

Resumen de: CN120361018A

The invention discloses a pharmaceutical composition or a vaccine for treating and/or inhibiting salmonella infection, and an application of a small molecule compound Epetrabole hydrochloride. The invention further discloses an application of the pharmaceutical composition or the vaccine and the small molecule compound Epetrabole hydrochloride for treating and/or inhibiting salmonella infection. The MIC value of the small molecule compound is 0.48 mu M, and compared with existing antibiotics for clinically treating salmonella enteritidis infection, the small molecule compound has relatively strong antibacterial activity when the small molecule compound is in a trace amount. An in-vitro killing bacteriostatic activity experiment result shows that the small molecule compound has a certain effect on reduction of the number of bacteria. The Salmonella enteritidis strain has obvious antibacterial activity on a J774A.1 cell model, and can inhibit the expression of a salmonella enteritidis virulence factor T3SS1 in a targeted manner. According to the present invention, the small molecule compound Epetrabole hydrochloride provides a certain inhibition effect on salmonella isolates from different sources, and has a wide application value;

Compound preparation for preventing and treating bacterial diseases of livestock and poultry and preparation method thereof

Resumen de: CN120361208A

The invention discloses a compound preparation for preventing and treating bacterial diseases of livestock and poultry and a preparation method of the compound preparation. The compound preparation is prepared from the following components in parts by weight: 3.2 to 5.8 parts of astragalus polysaccharide powder, 2.9 to 5.3 parts of xylooligosaccharide powder, 2.5 to 4.8 parts of compound enzyme agent, 2 to 4.4 parts of compound bacterium agent, 1.5 to 3.6 parts of selenium yeast powder, 1.3 to 3.2 parts of egg yolk antibody, 1 to 2.1 parts of hydroxypropyl methyl cellulose, 0.35 to 0.8 part of chitosan and 0.3 to 0.65 part of montmorillonite. The compound preparation disclosed by the invention can effectively inhibit growth and reproduction of viruses such as riemerella anatipestifer, escherichia coli and salmonella, and is beneficial to regulation of immune functions of animals and improvement of body resistance of the animals.

一种四基因敲除减毒鼠伤寒沙门氏菌及其构建方法和应用

Resumen de: CN120366177A

本发明提供一种四基因敲除减毒鼠伤寒沙门氏菌及其构建方法和应用，所提供的一种四基因敲除减毒鼠伤寒沙门氏菌，是敲除了鼠伤寒沙门氏菌的relA、Asd、manA和sifA基因。本发明构建了四种毒力因子缺失的鼠伤寒沙门氏菌的减毒菌株ΔrelA‑ΔAsd‑ΔmanA‑ΔsifA SM14028，可在体外稳定传代。并经安全性评价试验检测，该菌株毒力无毒力，安全性极高。为了进一步评价ΔrelA‑ΔAsd‑ΔmanA‑ΔsifA SM14028是否能够抵抗亲本株的攻击，进行了免疫效力评价，结果显示，ΔrelA‑ΔAsd‑ΔmanA‑ΔsifA SM14028能够提供有效的免疫保护。因此，本发明的ΔrelA‑ΔAsd‑ΔmanA‑ΔsifA SM14028可作为一种有效的活疫苗或者活载体疫苗，为动物疫病的防控奠定基础。

抗原タンパク質または該タンパク質をコードする遺伝子を有する細胞外小胞およびその用途

Resumen de: CN119630803A

The present invention relates to an extracellular vesicle containing an antigen protein or a gene encoding the protein, and a use thereof, and more specifically, to an extracellular vesicle containing an antigen protein derived from a virus, a microorganism or a cancer cell or a gene encoding the protein, or a vaccine composition for preventing or treating viral infections, microbial infections or cancers comprising the same. The extracellular vesicle or the vaccine composition comprising the same according to the present invention is a platform applicable to various diseases, has excellent antigen-specific immune response induction effect and stability, and is expected to be effectively used in the field of vaccine development. The vaccines are useful for the prevention or treatment of various diseases including viral infections, microbial infections or cancers.

METHOD FOR TESTING IN-VITRO BACTERICIDAL FUNCTION OF TYPHOID VACCINE IMMUNE SERUM

Resumen de: WO2025152055A1

Provided is a method for testing the in-vitro bactericidal function of a typhoid vaccine immune serum, comprising: testing the in-vitro bactericidal function of a typhoid vaccine immune serum against Salmonella typhi bacteria by means of an opsonophagocytic killing assay, wherein when the phagocytic killing step of the opsonophagocytic killing assay is completed, the Salmonella typhi bacteria having undergone the phagocytic killing step are cultured at the pH of 7.5-9.5. The opsonophagocytic killing assay is applied to Gram-negative Salmonella Typhi bacteria for the first time, and the in-vitro bactericidal levels of typhoid vaccine immune serums are effectively and accurately evaluated.

Method for rapidly detecting salmonella pullorum by using fluorescent quantitative PCR (Polymerase Chain Reaction) and primer used by method

Resumen de: CN120350143A

The invention discloses a method for rapidly detecting salmonella pullorum by fluorescent quantitative PCR (polymerase chain reaction) and used primers, and the method for rapidly detecting salmonella pullorum by fluorescent quantitative PCR specifically comprises the following steps: (1) collecting a sample; (2) nucleic acid extraction; (3) preparing a reaction system; (4) running a sample detection program; and (5) detecting a test result and judging. According to the rapid detection method disclosed by the invention, an ultra-short-range PCR program is designed as follows: pre-denaturation is performed at 95 DEG C for 2 minutes; according to the present invention, with the program setting, the salmonella pullorum detection time is shortened by about 60% compared with the salmonella pullorum detection time of the commercially available kit and the commercial standard program, and the detection efficiency is substantially improved; the cost of a single detection reagent is reduced by 40% compared with that of an imported kit, and the economic cost is reduced; compared with a traditional culture method, the method has the advantages that the time is 3-5 days earlier, the detection result is reliable, the repeatability is good, and the method can be used for clinical diagnosis and monitoring of SP.

Poultry salmonella drug resistance detection equipment

Resumen de: CN120349878A

The poultry salmonella drug resistance detection equipment comprises a bottom plate, the upper end of the bottom plate is fixedly connected with a first vertical plate and a second vertical plate which are arranged left and right, the upper end of the bottom plate is provided with a positioning frame located between the first vertical plate and the second vertical plate, and the upper end of the positioning frame is provided with a storage mechanism; a discharging opening located in the inner side of the positioning frame is formed in the bottom plate in a penetrating mode, a bearing mechanism is arranged on the inner side of the discharging opening, a positioning ring located on the left side of the positioning frame is arranged at the upper end of the bottom plate, a guide rod is fixedly connected between the first vertical plate and the second vertical plate, and the guide rod is sleeved with a sliding sleeve in a sliding mode. According to the device, continuous operation of taking and moving the drug sensitive paper and pasting the drug sensitive paper into the culture dish can be completed, mechanical linkage is utilized, reliability is good, independent driving is not needed, cost is reduced, the situation that the drug sensitive paper is manually taken out of the containing plates in sequence and then taken for pasting operation is not needed, the experiment efficiency is improved, and meanwhile the pollution risk is reduced.

Lactobacillus plantarum with broad-spectrum antibacterial function

Resumen de: CN120349938A

The invention discloses a broad-spectrum antibacterial lactobacillus plantarum, and belongs to the technical field of microorganisms. The lactobacillus plantarum 9-6 is separated from animal feed, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32568, the lactobacillus plantarum 9-6 and fermentation liquor thereof have an obvious inhibition effect on common vibrio parahaemolyticus, vibrio alginolyticus, vibrio harveyi and vibrio vulnificus, and the lactobacillus plantarum 9-6 and the fermentation liquor thereof have the advantages that the lactobacillus plantarum 9-6 and the fermentation liquor thereof have the obvious inhibition effect; and in addition, the antibacterial agent has a relatively good antibacterial effect on common staphylococcus aureus, escherichia coli, listeria monocytogenes, salmonella enterica and bacillus cereus. The lactobacillus plantarum 9-6 has probiotic safety, can grow under an initial pH condition of 4-10 and a high-salt environment, and plays a role in bacteriostasis under an acidic condition. The invention lays a foundation for developing a good antibacterial probiotic preparation and application of a fermentation culture thereof.

Radioiodine-labeled drug-loaded bacterial outer membrane vesicle, preparation method and application

Resumen de: CN120324371A

The invention relates to a radioiodine-labeled drug-loaded bacterial outer membrane vesicle as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The preparation method comprises the following steps: expressing casein-rich attenuated salmonella typhimurium VNP20009 to secrete bacterial outer membrane vesicles, loading an anti-tumor drug by using an ultrasonic method, and combining with an oxidant-mediated radioiodine labeling technology to construct radioiodine-labeled drug-loaded bacterial outer membrane vesicles with tumor targeting property, so as to prepare the drug-loaded bacterial outer membrane vesicles. The performance is optimized through polyethylene glycol modification, the drug loading mass ratio is controllable, the radiolabeling rate is high, the in-vitro stability is good, and the in-vivo safety is good. The drug-loaded bacterial outer membrane vesicle can simultaneously realize SPECT/PET imaging and targeted therapy, is suitable for solid tumors such as brain glioma, colon cancer and the like, has the functions of drug delivery, immune activation and radiodiagnosis, has a good application prospect, and provides a new tool for diagnosis and treatment of tumors.

Bacillus altitudinis MLF-1 as well as complex microbial inoculant and application thereof

Resumen de: CN120330108A

The invention relates to the technical field of microorganisms, in particular to bacillus altitudinis MLF-1 and a complex microbial inoculant and application thereof.The bacillus altitudinis strain MLF-1 is obtained by being separated from healthy cattle rectum contents by a research group, and it is detected that the strain has the good beta-1, 4-glucanase producing capacity, and the beta-1, 4-glucanase producing capacity is high; the antibacterial effect is achieved on enterobacter aerogenes, staphylococcus aureus, salmonella typhimurium and escherichia coli; the bacillus altitudinis MLF-1 has good fermentation capacity on the siraitia grosvenorii residues, after the bacillus altitudinis MLF-1 is mixed with the bacillus licheniformis strain JSF-9 and the bacillus safensis strain MLL-5 to prepare the complex microbial inoculant, the nutritional value and the disease resistance of the siraitia grosvenorii residues fermented by the complex microbial inoculant are remarkably improved, 10%-15% of the complex microbial inoculant is added into a basic ration, and the content of the complex microbial inoculant in the siraitia grosvenorii residues fermented by the complex microbial inoculant is reduced. The Jersey cattle feed has the effects of reducing cost and improving efficiency for feeding Jersey cattle.

Primer probe combination, kit and method for detecting salmonella enteritidis

Resumen de: CN120330356A

The invention provides a primer probe combination, a kit and a method for detecting salmonella enteritidis, and belongs to the technical field of rapid detection of pathogenic microorganisms. A series of primer probes are designed aiming at the Prot6e gene of the salmonella enteritidis, a primer probe combination with the best amplification effect is screened from the primer probes, and the salmonella enteritidis is detected by utilizing the primer probe combination and adopting a fluorescent RAA method. The detection efficiency, sensitivity (the lowest detection limit is 1.5 * 10 <-1 > pg/mu L) and accuracy of the detection method are all higher than those of common PCR, and rapid specific detection of salmonella enteritidis can be achieved.

Method for obtaining overexpressed TXNIP macrophage cell strain and application of overexpressed TXNIP macrophage cell strain in antibacterial research

Resumen de: CN120330263A

The invention belongs to the technical field of biology, and particularly relates to a construction method of a TXNIP gene overexpressed macrophage cell strain and application of the TXNIP gene overexpressed macrophage cell strain in salmonella typhimurium infection.The TXNIP gene overexpressed RAW264.7 macrophage cell strain (oeTXNIP-RAW264.7) is successfully constructed by utilizing CAGG-TXNIP recombinant plasmids and combining with a lentivirus expression system, and the TXNIP gene overexpressed RAW264.7 macrophage cell strain (oeTXNIP-RAW264.7) can be used for treating salmonella typhimurium infection. The gene overexpression effect is verified through an RT-qPCR (Reverse Transcription-Quantitative Polymerase Chain Reaction) method and a Western blot method. A design test proves that after the over-expression TXNIP vector is constructed and transferred into RAW264.7, compared with a control group (a CAGG empty vector is transferred into RAW264.7), infection of salmonella can be remarkably resisted. The invention has the characteristics of strong innovation, good safety and the like, provides a reliable technical platform for antibacterial target screening and innovative drug research, and has important application value in the field of biomedicine.

Yeast microcapsule delivery BBR/EGCG probiotic preparation and application

Resumen de: CN120324365A

The invention belongs to the field of biotechnology and medical preparations, and provides a yeast microcapsule delivery BBR/EGCG probiotic preparation and application thereof. The probiotic Nissle1917 and the anti-inflammatory and anti-oxidation natural products BBR and EGCG are packaged by adopting the yeast membrane, so that the probiotic Nissle1917 has good tolerance and cell targeting property, is easy to colonize in intestinal tracts and exert anti-inflammatory and mucous membrane repairing functions, particularly has a better treatment effect on enteritis caused by salmonella typhimurium, and has a wide application prospect. The problem that an existing probiotic agent is poor in bacterial enteritis treatment effect is solved.

Primer probe combination, kit and method for detecting salmonella pullorum

Resumen de: CN120330357A

The invention provides a primer probe combination, a kit and a method for detecting salmonella pullorum, and belongs to the technical field of rapid detection of pathogenic microorganisms. A series of primer probes are designed aiming at the ipaJ gene of the salmonella pullorum strain, a primer probe combination with the best amplification effect is screened from the primer probes, and the salmonella pullorum is detected by utilizing the primer probe combination and adopting a fluorescent RAA method. The detection efficiency, sensitivity (the lowest detection limit is 1.5 * 10 <-1 > pg/mu L) and accuracy of the detection method are higher than those of common PCR, and rapid specific detection of salmonella pullorum can be achieved.

ENTEROTOXIGENIC ESCHERICHIA COLI COLONIZATION FACTOR CS2-CS3 EXPRESSION IN ATTENUATED SHIGELLA LIVE VECTORS

Resumen de: WO2025151522A2

A broadly protective Shigella-ETEC vaccine includes components to cover multiple Shigella species and serotypes as well as multiple different colonization factors of ETEC. ETEC colonization factors CS2 and CS3 are among the most prevalent CFs found on ETEC isolates associated with diarrhea. These two factors are important components of a vaccine that can confer broad protection against ETEC. High level expression of these heterologous antigens in Shigella live vectors results in stronger immune responses. This specification describes the genetic engineering of DNA sequences encoding upstream sequences, including promoter and ribosome binding sites, that results in increased expression of CS2 and CS3 in Shigella live vectors and that leads to stronger immune responses when used as a vaccine in animal models.

LIVE SALMONELLA TYPHI VECTORS ENGINEERED TO EXPRESS CANCER PROTEIN ANTIGENS AND METHODS OF USE THEREOF

Nº publicación: EP4583908A2 16/07/2025

Solicitante:

UNIV MARYLAND [US]
IRAZU ONCOLOGY LLC [US]
University of Maryland, Baltimore,
Irazu Oncology, LLC

Resumen de: AU2023338191A1

The present invention provides compositions and methods for inducing an immune response in a subject in need thereof, comprising administering to the subject an immunologically-effective amount of a live