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95
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Última actualización
31/08/2024 [08:13:00]
Resultados 25 a 50 de 95
Resumen de: CN118362722A
本申请公开了一种沙门菌筛选方法及其应用，涉及沙门菌分离领域，其包括以下步骤：将活化磁珠与抗体共培养得到偶联磁珠，将偶联磁珠与样品混匀孵育后分离出结合有沙门菌的偶联磁珠，使用抗体标记去除试剂与结合有沙门菌的偶联磁珠混合孵育，分离出磁珠得到筛选出的沙门菌悬浮液；所述抗体包括PagN单克隆抗体、PagC单克隆抗体、FimA单克隆抗体和FimH单克隆抗体中的至少两种。本申请能提高沙门菌筛选的特异性和效率。
Resumen de: US2024240140A1
Disclosed are attenuated Salmonella gallinarum expressing FliC (flagellin) or FliC-hIL2 (human IL2 fused to FliC) and use thereof, wherein the Salmonella strain exhibits excellent immune activation ability and anticancer efficacy and thus can be used as a cancer therapeutic agent, together with or independently from existing anticancer agents.
Resumen de: AU2022343176A1
Provided herein is the ability for intravenous delivery of bacteria without the septic side-effects and for delivery of immune modulating proteins that are systemically toxic to be delivered directly to the tumor micro-environment without any systemic toxicity.
Resumen de: WO2023047129A1
The invention relates to fusion polypeptides, and to immunogenic polypeptides and their use as vaccines for treating, preventing or ameliorating a wide range of infectious diseases, for example caused by a virus, bacterium or fungus, or cancer. The fusion polypeptide comprises: a first amino acid sequence comprising a non-toxic B subunit of an AB5 Toxin, or a fragment or variant thereof; a second amino acid sequence comprising an immunoglobulin Fc region (Ig-Fc), or a fragment or variant thereof; and a third amino acid sequence comprising an antigen, or a fragment or variant thereof. The invention also extends to nucleic acids encoding such fusion polypeptides and proteins, and to recombinant vectors expressing such nucleic acids. The invention is especially useful for the rapid development of protein-based vaccines, and to their use in methods of treating infectious diseases or cancer, and also to pharmaceutical compositions comprising the fusion proteins.
Resumen de: US2024226190A1
The present invention relates to the field of cancer therapy. In particular, the present invention relates to a first composition comprising a live attenuated bacterium for use in the treatment, prevention, reduction, inhibition, prevention of recurrence, or control of a neoplastic disease in a subject being or intended to be administered a second composition comprising a live attenuated bacterium, said bacterium being the same or different to that of the first composition and methods thereof.
Resumen de: WO2024146625A1
A duplex fluorescent PCR detection primer probe group of duck Salmonella enteritidis and Salmonella typhimurium, a kit, and the use thereof. Comparative analysis on the whole genomes of Salmonella enteritidis and Salmonella typhimurium is performed to screen out as identification targets a specific fragment Sdf I of lyg D gene of Salmonella enteritidis and a specific gene STM4497 of Salmonella typhimurium, and the present application designs specific primers and probes.
Resumen de: WO2024148143A1
Methods and antimicrobial compositions are provided for surface cleaning. The antimicrobial compositions which include very low concentrations of an organic acid and one or more anionic surfactants can, unexpectedly, provide effective and broad spectrum antimicrobial activity within two minutes of contact with a surface and in some instances within one minute of contact time. Contacting the antimicrobial compositions with a surface can result in greater than or equal to about 4.0 log kill or 5.0 log kill for one or more of Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica on the surface in two minutes or less. The compositions can have antimicrobial activity against at least one gram-positive bacterium, gram-negative bacterium, virus, and fungus in two minutes or less of contacting the composition with a surface.
Resumen de: US2024228910A1
Methods and antimicrobial compositions are provided for surface cleaning. The antimicrobial compositions which include very low concentrations of an organic acid and one or more anionic surfactants can, unexpectedly, provide effective and broad spectrum antimicrobial activity within two minutes of contact with a surface and in some instances within one minute of contact time. Contacting the antimicrobial compositions with a surface can result in greater than or equal to about 4.0 log kill or 5.0 log kill for one or more of Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica on the surface in two minutes or less. The compositions can have antimicrobial activity against at least one gram-positive bacterium, gram-negative bacterium, virus, and fungus in two minutes or less of contacting the composition with a surface.
Resumen de: US2024229100A1
Disclosed herein are methods and systems for rapid detection of microorganisms in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as Salmonella-specific bacteriophage, allows detection of a specific microorganism, such as Salmonella spp. and an indicator signal may be amplified to optimize assay sensitivity.
Resumen de: KR20240107309A
본 발명은 돼지 부종병 ELISA 키트 및 상기 키트를 이용한 돼지 부종병의 진단방법에 관한 것으로, 보다 상세하게는 시가 독소(shiga toxin) 재조합 항원 및/또는 상기 재조합 항원 특이적 항체를 포함하는 효소면역측정법 기반 돼지 부종병 진단 키트에 관한 것이다. 본 발명에서는 시가 독소(shiga toxin) 유래 재조합 항원(HJP2 항원) 및 상기 항원 특이적인 재조합 항체를 제조하였으며, 이를 이용하여 간접면역효소면역측정법(indirect ELISA) 기반 및 샌드위치 효소면역측정법(sandwich ELISA) 기반 돼지 부종병 진단 키트를 제조하였다. 본 발명에서 제조한 진단 키트는 돼지 혈청 속에 포함된 시가 독소에 대한 항체를 효과적으로 인식하는 것을 확인하였으므로, 돼지 부종병 진단에 유용하게 활용할 수 있다.
Resumen de: AU2022375120A1
An immunogenic gel compositions for oral administration and methods of immunizing an animal the methods including administering to the animal a therapeutically effective amount of an immunogenic gel composition comprising an antigen of an animal pathogen and a gel composition for oral administration.
Resumen de: US2024215608A1
Formaldehyde is a common component of antimicrobial compositions for preserving animal feeds. However, the use of formaldehyde is coming under pressure and alternative antimicrobial compositions are needed. The present application discloses methods and/or uses of a formaldehyde-free antimicrobial composition comprising formic acid and propionic acid in the making of rendered animal protein compositions or in the inhibiting of the development of Salmonella bacteria in animal by-product compositions (e.g., rendered animal proteins) while maintaining higher protein digestibility comparing with formaldehyde.
Resumen de: US2024218318A1
The present invention relates to an attenuated Salmonella gallinarum strain and use thereof. Particularly, the present invention relates to: a novel attenuated Salmonella strain in which all of a gene encoding guanosine tetraphosphate (ppGpp) synthetase, a gene that induces the function of a type III secretion system (T3SS)(ssrAB), and a Gifsy 2prophage gene are deleted; and a composition for treating or diagnosing tumors by using same.
Resumen de: KR20240103277A
본 발명은 살모넬라균의 증균을 위해 사용되는 최소배지로서, 상기 최소배지는 살모넬라균의 유전적 특성을 고려하여 그에 대한 성장촉진인자로 탄소 성분, 질소 성분 및 무기질 성분을 포함하는 것을 특징으로 하는 살모넬라균 증균을 위한 최소배지 및 이를 이용한 살모넬라균 증균방법으로서, 짧은 증균시간에 살모넬라균의 수를 대폭 증가시켜 높은 증균 효과를 제공할 수 있다.
Resumen de: WO2023025815A1
The present invention relates to immunogenic compositions and their use in providing protection against illness caused by infection with Shigella. The immunogenic compositions comprise Shigella GMMA with particular doses of O-antigen.
Resumen de: CN118266495A
本发明涉及农业生物技术领域，具体涉及一种用于杀灭沙门氏菌的复合酶系及其应用。本发明的复合酶系包括纤维素酶、高丝氨酸内酯酶AiiO、磷酸化酶LsrK以及沙门氏菌素SalE1a。该复合酶系通过降解沙门氏菌生物膜、破坏沙门氏菌群体感应系统以及杀灭沙门氏菌病原体，有效清除沙门氏菌的鸡肉污染，改善肉品质，延长鸡肉保鲜期；同时在在小鼠沙门氏菌感染模型中，使用该复合酶系可抑制沙门氏菌生长、定植以及致病，降低炎症反应，保护肠道屏障，恢复肠道菌群稳态。
Resumen de: CN118256638A
The invention relates to a specific nucleic acid probe, a nucleic acid enrichment method and a formed salmonella detection method, the specific nucleic acid probe is designed and synthesized by salmonella Inva gene, and comprises a capture probe of which the 5'end is modified with amino and is used for connecting carboxyl magnetic beads and a detection probe of which the 3 'end is modified with TAMRA-N and is used for signal detection; wherein the 3'end of the capture probe and the 5 'end of the detection probe are respectively combined with a specific nucleic acid sequence of salmonella to be detected to form a compound of a magnetic probe, a nucleic acid fragment to be detected and the detection probe; to-be-detected nucleic acid fragments are extracted and enriched by the established nucleic acid enrichment and purification technology capable of amplifying signals, and finally the concentration of the salmonella is obtained by detecting the fluorescence intensity and the standard curve, so that the aim of specifically and quantitatively detecting the salmonella is fulfilled. The salmonella detection method provided by the invention does not need enrichment, has high specificity and high sensitivity, can detect trace salmonella in an actual sample (complex environment), improves the detection sensitivity, and improves the detection efficiency at the same time.
Resumen de: CN118252181A
本发明涉及农业生物技术领域，具体涉及一种用于杀灭沙门氏菌的复合酶系及其应用。本发明的复合酶系包括纤维素酶、高丝氨酸内酯酶AiiO、磷酸化酶LsrK以及沙门氏菌素SalE1a。该复合酶系通过降解沙门氏菌生物膜、破坏沙门氏菌群体感应系统以及杀灭沙门氏菌病原体，有效清除沙门氏菌的鸡肉污染，改善肉品质，延长鸡肉保鲜期；同时在在小鼠沙门氏菌感染模型中，使用该复合酶系可抑制沙门氏菌生长、定植以及致病，降低炎症反应，保护肠道屏障，恢复肠道菌群稳态。
Resumen de: CN118256507A
The invention belongs to the technical field of biosensors, and provides a salmonella biosensor of a CRISPR/Cas12a system activated by DNAzyme, and the salmonella biosensor comprises a composite structure Apt-T, crRNA, a Blocker, the CRISPR/Cas12a system, an FQ chain and a substrate chain S; the Apt-T composite probe is obtained by hybridizing an aptamer Apt and a T chain; the Apt, the T chain, the crRNA, the Blocker chain, the substrate chain S and the reporter probe FQ are as shown in SEQ ID NO: 1-5. The sensor disclosed by the invention has the advantages of high detection efficiency, sensitivity, strong selectivity and the like, can make up the defects and deficiencies of the existing detection method of salmonella, and realizes rapid and accurate quantitative detection of salmonella.
Resumen de: US2024209068A1
The present application is directed to compositions comprising RNA having an open reading frame encoding an antibody heavy chain, an RNA having an open reading frame encoding an antibody light chain and an RNA having an open reading frame encoding an antibody J-chain, wherein the ratio of the RNAs is effective in producing an assembled antibody capable of forming a multimer. Compositions comprising RNA having an open reading frame encoding an IgG3 heavy chain, an RNA having an open reading frame encoding an antibody light chain, wherein the ratio of RNAs is effective in producing an assembled antibody are defined. Methods of producing therapeutic levels of IgA or IgM or IgG3 in vivo are also defined. The use of a lipid nanoparticle delivery vehicle is disclosed to administer the RNAs in vivo.
Resumen de: WO2024137831A2
A composition of bacteriophages comprising at least one phage selected from TTS1, TTS2, TTS3, TTS4, TTS5, and TTS6, wherein the phages are optionally encapsulated; and a method for preventing or treating an infection caused by Salmonella Gallinarum by administering to a subject a therapeutically effective amount of the composition.
Resumen de: CN118240687A
The invention discloses a hen lactobacillus and probiotic agent, freeze-dried bacterial powder and application thereof. The Latin name of the lactobacilli hens is Lactobacillus gallinarum, the lactobacilli hens are named as HNAU2301, and the preservation number of the lactobacilli hens in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms is CGMCC NO.29242; the nucleotide sequence of 16SrDNA (deoxyribonucleic acid) of the gene is SEQ ID NO. 1. The lactobacillus hennis HNAU2301 provided by the invention is relatively weak in acid resistance and bile salt resistance, relatively good in self-aggregation and biological membrane formation capability and relatively good in inhibition capability on salmonella pullorum; therefore, antibiotics can be replaced, and damage of the antibiotics to organisms is reduced. When the hen lactobacillus provided by the invention is added into a feed, the serum IgY level and egg yolk IgY level of breeding hens can be remarkably increased, the fertilization rate and hatching rate of hatching eggs of breeding hens can be improved, and the dead embryo rate can be reduced, so that the production performance of the breeding hens is improved.
Resumen de: AU2022375120A1
An immunogenic gel compositions for oral administration and methods of immunizing an animal the methods including administering to the animal a therapeutically effective amount of an immunogenic gel composition comprising an antigen of an animal pathogen and a gel composition for oral administration.
Resumen de: CN118221760A
本发明提供了一种痢疾多糖蛋白结合物原液的制备方法，其特征在于，所述方法，包括以下步骤：(1)取痢疾多糖蛋白结合物原液粗品，用0.85％氯化钠溶液先稀释后浓缩至一定体积，使用切向流超滤膜包进行超滤，超滤液浓缩后得到浓缩液；(2)用0.85％氯化钠溶液平衡层析柱，将步骤(1)的浓缩液离心后上柱，收集洗脱液。
Nº publicación: CN118222459A 21/06/2024
Solicitante:
LANZHOU VETERINARY RES INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES CHINA CENTER FOR ANIMAL HE
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Resumen de: CN118222459A
The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus plantarum with efficient bacteriostatic and anti-inflammatory effects and application of the lactobacillus plantarum. The invention provides a strain of lactobacillus plantarum (Lactobacillus plantarum) F1m, and the preservation number of the lactobacillus plantarum F1m is CGMCC (China General Microbiological Culture Collection Center) NO.29344. The lactobacillus plantarum F1m has the advantages that the preservation number of the lactobacillus plantarum F1m is CGMCC NO.29344; the strain is separated from yak yoghurt traditionally fermented in Qinghai province, has good tolerance to acid and cholate environments, has high adhesiveness to epithelial cells Caco-2, and can significantly inhibit growth of common pathogenic bacteria such as staphylococcus aureus, salmonella and escherichia coli. The lactobacillus plantarum F1m can be used for remarkably improving DSS-induced colitis symptoms, reducing inflammatory response and repairing damaged intestinal barriers after being used for gavage of mice with colitis. The development of the strain not only excavates high-quality germplasm resources in yak yoghurt, but also provides certain guarantee for the health of human and animals.
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