Microalgas: Cultivo y usos

Rapid biofilm culturing device for suspended carrier of algal-bacterial symbiotic biofilm for sewage treatment

Resumen de: CN221027955U

The utility model provides a rapid biofilm culturing device for a suspended carrier of a bacteria-algae symbiotic biofilm for sewage treatment, which comprises a base, a medicine dissolving tank fixedly mounted on one side of the top of the base, and a microorganism biofilm culturing tank fixedly mounted on the top of the base and positioned on one side outside the medicine dissolving tank, the top of the base is fixedly provided with a microalgae attachment pond which is fixedly arranged with the microorganism biofilm culturing pond. According to the rapid biofilm culturing device of the bacterial-algae symbiotic biofilm suspension carrier for sewage treatment, provided by the utility model, rapid and efficient batch biofilm culturing of the bacterial-algae biofilm suspension carrier is realized, and the biofilm culturing of the suspension carrier is realized in the microbial biofilm culturing pool by utilizing different functions of the microbial biofilm culturing pool and the microalgae attachment pool; the suspended carrier is transferred to the microalgae attachment pond, the microalgae are attached to a biological membrane on the surface of the suspended carrier, a carrier cage containing the suspended carrier can be placed in the microorganism biofilm culturing pond and the microalgae attachment pond at the same time, and enrichment of microorganisms and the microalgae is achieved synchronously.

SEMI-SPHERICAL PHOTOBIOREACTOR FOR PRODUCING MICROALGAE.

Resumen de: MX2022014793A

The present invention relates to a hemispherical photobioreactor for producing microalgae supported on a main base that incorporates a pair of fastening posts that support a photobioreactor by a rotation mechanism, rotating the reaction chamber, which is hemispherical and has an air inlet for the connection of an external air conductor from a compressor coupled with an internal air conductor in the reaction chamber, promoting the entry of an air current into the interior of the reaction chamber. Also, it incorporates an oxygen valve in each of the poles of the reaction chamber, controlling the opening or closing from a control center and the rotation of the motive medium coupled with the reaction chamber and the air supply.

Apparatus for Cultivation of Microalgae and Cultivation Method Thereof

Resumen de: KR20240073585A

본 발명은 미세조류의 종류에 상관없이 미세조류의 배양 효율 및 배양 수율이 향상되고, 배양챔버의 상태를 LED 램프로 시각화하여 비상시 대응이 용이할 뿐만 아니라 에너지 절감 효과가 있는 미세조류 배양장치 및 이를 이용한 배양방법을 개시한다.

Microalgae cultivation test device convenient for sampling

Resumen de: CN118064252A

The invention provides a microalgae cultivation test device convenient for sampling, and belongs to the field of microculture. The device comprises a shell, a driving system, a nutrition system, a breeding frame and a sampling system. The outer layer of the breeding frame is sleeved with a breeding carrier, the driving system can drive the breeding carrier to rotate around the breeding frame, and in the process that the breeding carrier circularly rotates around the breeding frame body, the breeding carrier can obtain microalgae seed liquid and culture liquid from the nutrition system. The sampling system comprises sliding locking heads arranged on the two sides of the breeding bin, the rope penetrates into the inner side of the breeding bin from one sliding locking head, then sequentially passes through the lower edge of the breeding frame to reach the other sliding locking head and then penetrates out of the breeding bin to be fixed, and when the rope is in extrusion contact with the surface of the breeding frame and a breeding carrier on the surface of the breeding frame rotates, the breeding carrier rotates. The rope can scrape the microalgae from the surface of the breeding carrier, and the microalgae are conveyed to the receiving box through the conveying belt below the breeding frame. According to the device, microalgae can be conveniently collected and picked, and the growth and cultivation environment of the microalgae cannot be affected in the process.

Method for screening transgenic microalgae by using flow cytometry

Resumen de: CN118064484A

The invention discloses a method for screening transgenic microalgae by using a flow cytometer, which comprises the following steps: step S1, constructing a recombinant plasmid expression vector which contains a target gene, a gene series of VENUS protein and a resistance gene; s2, transferring the recombinant plasmid expression vector in the step S1 into wild microalgae through an electric shock conversion method, and culturing; step S3, mixing at least two monoclonal microalgae in the step S2 by using a sterilized inoculating loop, transferring the mixed microalgae into a TAP culture medium, and culturing; step S4, analyzing the monoclonal microalgae mixed in the step S3 through a flow cytometer; and step S5, carrying out PCR (Polymerase Chain Reaction) technology detection and antigen-antibody hybridization technology detection on each monoclonal microalgae in the mixed monoclonal microalgae of which the fluorescent protein signals are detected in the step S4. The screening device has the advantage of high screening efficiency.

Microalgae immobilized culture control test device

Resumen de: CN118064241A

The invention provides a control test device for immobilized culture of microalgae, and belongs to the technical field of microbial culture. The device mainly comprises a breeding bin, a nutrition bin, a rotating shaft, a breeding frame and a nutrition system. The rotating shaft is arranged at the top of the breeding bin, the hard pipe of the nutrition system is arranged below the rotating shaft, and the breeding frame is arranged between the rotating shaft and the hard pipe in a sleeved mode. The breeding frame comprises a body, a bearing plate, a breeding carrier and a contact ring, when the rotating shaft rotates, the breeding carrier is attached to the bearing plate and circularly rotates around the body, and through liquid passing grooves are formed in a first containing cavity and a second containing cavity in the bottom of the body; microalgae seed liquid and culture liquid are put into the seed liquid pipe and the nutrient pipe through the spraying holes, and the culture carrier is infiltrated through the liquid passing groove. The culture carrier slowly absorbs the culture solution when rotating and passing through the liquid passing groove, automatic culture solution supplement is achieved, the adherence effect of microalgae cannot be damaged in the supplement process, and therefore a control test is easier to implement, fewer influence factors exist, and the conclusion is more reliable.

Circulating microalgae culture device for oyster breeding

Resumen de: CN220999649U

The utility model discloses a circulating microalgae culture device for oyster breeding, which comprises a stepped rack, an aeration mechanism, a heating mechanism, an illumination mechanism and an electromagnetic valve, the stepped rack is provided with culture ponds, in every two adjacent culture ponds, the culture pond at the high-order position is provided with a water outlet pipe with the electromagnetic valve, and the water outlet pipe is provided with a water inlet pipe with the electromagnetic valve. The highest-order culture pond is provided with a water inlet pipe with an electromagnetic valve, and the lowest-order culture pond is connected with a water suction pump; the aeration mechanism and the heating mechanism are respectively connected with the plurality of culture ponds. The culture ponds are arranged in a stepped mode and matched with the water outlet pipe and the electromagnetic valve, automatic pipetting operation of the culture ponds can be achieved, the labor intensity of workers can be relieved, and microalgae culture is easy and convenient; the aeration mechanism realizes oxygenation of the culture pond and flowing of culture liquid, so that microalgae growth is facilitated; the temperature and illumination intensity of the culture pond can be controlled by arranging the illumination mechanism and the heating mechanism, and it can be guaranteed that the temperature and illumination intensity of the culture pond are suitable for microalgae growth.

Microalgae culture device and microalgae culture method

Resumen de: CN118064248A

The invention belongs to the technical field of microalgae culture, and particularly relates to a microalgae culture device which comprises a transparent tank body, the diameter of the tank body is gradually increased from top to bottom, the top of the tank body is open, a discharge pipe is connected to the center of the bottom of the tank body, and the diameter of the discharge pipe is gradually increased from top to bottom. A tank cover is arranged at the top of the tank body, and a transparent lampshade is rotatably inserted in the center of the top of the tank cover in a penetrating manner. In the microalgae culture process, gas generated by the aeration disc can float upwards along the inner wall of the top of the groove and the inner wall of the tank body, so that a circle of gas ring is formed on the inner wall of the tank body, and meanwhile, the scraping strip can scrape by taking the lampshade as the circle center to be attached to the inner wall of the tank body under the action of the gas; therefore, the microalgae and the impurities are further prevented from being attached to the inner wall of the tank body by matching with a gas ring formed by the aeration disc, so that the outside light can be better irradiated into the tank body.

Method for repairing aquaculture tail water by using photosynthetic bacteria

Resumen de: CN118063000A

The invention relates to the technical field of aquaculture tail water treatment, in particular to a method for repairing aquaculture tail water by using photosynthetic bacteria. The invention relates to a method for repairing aquaculture tail water by using photosynthetic bacteria. The method comprises the following steps: inoculating a microalgae-microorganism symbiont onto an immobilized nanoparticle carrier; introducing into a bioreactor, introducing the aquaculture tail water into the bioreactor, and domesticating for a long time; the method comprises the following steps: planting aquatic plants in a bioreactor, and inoculating activated sludge into the bioreactor; and operating the bioreactor, detecting water quality parameters of the water outlet, and adjusting the parameters of the bioreactor. According to the method, a tail water remediation system with high efficiency and good compatibility is constructed, the immobilized carrier particles fix the microalgae-microorganism symbionts, the concentration and stability of microorganisms are improved, and the tail water remediation efficiency is improved by combining the absorption and degradation effects of the algae-microorganism symbionts and aquatic plants and the microbial metabolic activity of activated sludge. The comprehensive and efficient removal of pollutants such as ammonia nitrogen and organic matters in the aquaculture tail water is realized.

METHODS OF PREPARATION OF A FOOD OR A FEED PRODUCT OR INGREDIENT FROM NON-REFINED MICROALGAE & USES THEREOF

Resumen de: WO2024105258A1

The invention relates to a method for the preparation of microalgal for use as a food-grade ingredient without the need of any further purification or separation step and resulting products therefrom.

MICROALGAE COMPOSITIONS FOR HOST PLANT NUTRIENT UTILIZATION, ABIOTIC STRESS, AND SOIL FITNESS

Resumen de: AU2022397556A1

The present disclosure provides methods for improving the nutrient utilization, resistance to abiotic stress, and recovery from abiotic stress of a host plant by applying a microalgae composition to the host plant. The disclosure also provides methods for improving soil fitness and improving rhizospheric activity and/or growth by applying a microalgae composition to the soil.

EUKARYOTIC ALGAE COMPOSITIONS AND METHODS THEREOF

Resumen de: AU2022381194A1

The present disclosure provides recombinant polynucleotide comprising a heterologous regulatory element operably linked to a first nucleic acid sequence encoding a cyanobacterial bicarbonate transporter protein. The disclosure also provides expression vectors comprising the recombinant polynucleotides as well as eukaryotic algae and methods of utilizing the eukaryotic algae for improved carbon removal and biomass production.

NOVEL STRAIN OF SCHIZOCHYTRIUM SP. WITH EASY INTRACELLULAR OIL EXTRACTION AND A METHOD FOR PRODUCING OIL CONTAINING OMEGA-3 USING SAME.

Resumen de: MX2024005588A

The present invention relates to a novel strain of <i>Schizochytrium</i> sp. with easy intracellular oil extraction and a method for producing oil containing omega-3 using same. The novel microalgae of Schizochytrium sp. according to the present invention have a high fat content compared to other biomass and especially have a high content of unsaturated fatty acids such as docosahexaenoic acid and eicosapentaenoic acid, and thus allow easy extraction of biomass produced from the microalgae or by culturing and fermenting, and easy extraction of fat components including unsaturated fatty acid from the biomass. Therefore, the microalgae, and a dried biomass product and bio-oil produced therefrom may be utilized as a composition for feed or a composition for food.

ORGANIC SELENIUM ENRICHED EDIBLE MARINE MICROALGAL BIOMASS

Resumen de: US2024164418A1

The present disclosure provides an organic selenium enriched edible marine microalga Nannochloropsis oceanica CASA CC201 with essential micronutrient Selenium, comprising organic selenium, along with omega 3 fatty acid and mono unsaturated fatty acid (MUFA). The present disclosure also provides a process for the organic selenium enrichment of edible marine microalga and a composition comprising the organic selenium enriched edible marine microalga Nannochloropsis oceanica. Moreover, the present disclosure also discloses a composition comprising the sustained bioprocess for organic selenium enriched edible marine microalga Nannochloropsis oceanica selenium enriched edible marine microalga, and wherein the composition is in the form of food, health, and feed supplements.

NOVEL STRAIN OF SCHIZOCHYTRIUM SP. WITH EASY INTRACELLULAR OIL EXTRACTION AND METHOD FOR PRODUCING OIL CONTAINING OMEGA3 USING SAME.

Resumen de: MX2024005590A

The present application relates to a novel strain of <i>Schizochytrium</i> sp. with easy intracellular oil extraction and a method for producing oil containing omega3 using same. The novel microalgae of Schizochytrium sp. according to the present invention has a high content of fat in biomass, specially a high content of unsaturated fatty acids such as docosahexaenoic acid and eicosapentaenoic acid, and thus it is very easy to extract biomass produced by itself or by cultivation and fermentation and fat components including unsaturated fatty acids from the biomass. Accordingly, the microalgae, and dried biomass and bio-oil prepared therefrom can be helpfully used as a composition for feed or a composition for food.

CULTURE SYSTEM

Resumen de: JP2024068690A

To provide a culture system that can prevent a damage of a culture tank when culture solution in the culture tank is sucked by a suction device, and can suppress a residual amount of culture solution and microalgae inside the culture tank as small as possible when recovering them.SOLUTION: A culture tank (20) constituting a culture system (10) is formed of material having flexibility. When recovering culture solution (L) from the culture tank, a recovery pipe (50) is housed in the culture tank. The recovery pipe is a cylindrical body having an internal space (68). The recovery pipe has a first recovery part (62) extending along a bottom part (30) of the culture tank. A first rib (76) is provided for an end part of the first recovery part. The first rib has a first protrusion part and a second protrusion part. The first protrusion part and the second protrusion part protrude from opposite sides of the outer peripheral surface of the first recovery part in parallel with a depth direction (X) of the culture tank.SELECTED DRAWING: Figure 1

Shellfish fry circulating water breeding/culturing system

Resumen de: CN118044484A

The invention discloses a shellfish fry circulating water breeding/culture system, which comprises an algae continuous culture system, a culture pond and a circulating water treatment system, the algae continuous culture system comprises a photobioreactor, a reactor main body supplies algae liquid to the culture pond through a guide pipe, and the culture pond is connected with the circulating water treatment system through a tail water pipe and a water inlet pipe. According to the shellfish fry circulating water breeding/culturing system, continuous culture of bait (microalgae) can be achieved, algae liquid is automatically fed into the culturing pond according to the shellfish growth stage and the culturing pond algae cell density, culturing water is circulated through the circulating water treatment system, industrial culturing of shellfish fry is achieved, the culturing procedures are reduced, and manual intervention is reduced.

Selenium-enriched microalgae premixed powder preparation device

Resumen de: CN220968724U

The utility model discloses a selenium-enriched microalgae premixed powder preparation device which comprises a mixing tank, wherein an output shaft of a first driving motor is fixedly connected with a first mixing rod through a coupling; through the design of structures such as a first driving motor, a first material mixing rod, a scraper blade and a second material mixing rod, and through the cooperation of the first driving motor, the first material mixing rod and the scraper blade, premixed flour in the material mixing tank and at the bottom in the material mixing tank can be stirred and mixed, and through the cooperation of a second driving motor and the second material mixing rod, the mixing efficiency is improved. According to the premixed flour mixing device, premixed flour close to the inner wall of the mixing tank can be mixed and stirred, the mixing effect of the premixed flour can be improved, uniform mixing of the premixed flour is guaranteed, the premixed flour can be sterilized by arranging an ultraviolet lamp, and the premixed flour can be stirred and turned over in the mixing process of the premixed flour, so that the ultraviolet lamp can better sterilize the premixed flour, and the mixing efficiency of the premixed flour is improved. Therefore, the sterilization effect on the premixed flour can be improved, and the premixed flour is ensured to be sterile.

Microalgae culture system based on recycling of tail gas generated by biomass garbage treatment

Resumen de: CN220976946U

The utility model discloses a microalgae culture system based on reutilization of tail gas generated by biomass garbage treatment, which relates to the technical field of energy conservation, emission reduction and microalgae culture and comprises a microalgae culture kettle, a heating jacket is arranged outside the microalgae culture kettle and connected with a tail gas inlet branch pipe and a tail gas outlet branch pipe, and the tail gas inlet branch pipe is connected with the tail gas outlet branch pipe. The tail gas inlet branch pipe and the tail gas outlet branch pipe are connected with a tail gas inlet header pipe, a dust removal device, a fan and a carbon dioxide storage tank are arranged on the tail gas inlet header pipe, the carbon dioxide storage tank is connected with a carbon dioxide inlet pipe and an aeration pipe, the aeration pipe is arranged in a microalgae culture kettle, and a nutrient solution inlet is formed in the microalgae culture kettle; a stirring mechanism with an illuminating lamp is arranged in the microalgae culture kettle, and a liquid outlet is formed in the lower part of the microalgae culture kettle. The tail gas preheating generated by domestic garbage treatment is used as a heat source to heat the microalgae culture kettle, and carbon dioxide collected from the treated tail gas is used as a carbon source for microalgae culture, so that a new direction is provided for tail gas reutilization, and energy conservation and emission reduction are

Method for enhanced treatment of high-salinity nitrogen methyl pyrrolidone wastewater by using halotolerant bacteria-algae symbiotic sludge system

Resumen de: CN118047482A

The invention discloses a method for enhanced treatment of high-salinity nitrogen methyl pyrrolidone wastewater by using a salt-tolerant bacteria-algae symbiotic sludge system. According to the algal-bacterial symbiotic sludge system, chlorella sorokiniana FACHB-275 is selected as a target microalgae, and activated sludge is taken from a secondary sedimentation tank of a chemical wastewater treatment plant. The algal-bacterial symbiotic sludge system has excellent salt tolerance, the system utilizes dissolved oxygen generated by microalgae photosynthesis as an electron acceptor to strengthen NMP degradation, CO2 and ammonia nitrogen generated by NMP degradation are supplied to microalgae for growth and utilization, high-salinity NMP wastewater can be strengthened under the non-aeration condition, and the system has the advantages of simple operation, low cost and high efficiency. And the efficient and stable treatment effect can still be kept under the high-salinity condition.

Slow-flow thin-layer photobioreactor

Resumen de: CN118028083A

The invention relates to the technical field of microalgae photobioreactors, and discloses a slow-flow thin-layer photobioreactor which comprises a supporting frame and a water tank container installed on the supporting frame, the water tank container is obliquely arranged, and a plurality of baffles arranged in a staggered mode are arranged in the water tank container in an array mode in the extending direction. Through cooperation of the baffles which are arranged in the water tank container and are arranged in a staggered mode, the flowing track of algae liquid is in an S shape, the flowing path of the algae liquid is greatly increased, and the retention time of the algae liquid in the water tank container is prolonged; the flow velocity required for maintaining the algae liquid with the same thickness is obviously smaller than that required when the baffle plates are not arranged, so that the energy consumption caused by overhigh flow velocity can be reduced, and the energy consumption can be greatly reduced by adjusting the distance between the two adjacent baffle plates and the width of the water tank reactor.

Fermentation tank suitable for fermentation culture of cell-wall-free microalgae

Resumen de: CN220951788U

The utility model relates to the technical field of microalgae fermentation tanks, and discloses a fermentation tank suitable for fermentation culture of cell-wall-free microalgae, which is characterized in that a fermentation cavity is arranged in a first tank body, a second tank body is arranged in the fermentation cavity, and a guide plate is arranged between the first tank body and the second tank body. The outermost side of the guide plate is tightly attached to the inner wall of the first tank body, a damping plate is fixedly installed at the bottom of the second tank body, and the air flowing mode and speed in the fermentation tank can be helped to be controlled by installing the guide plate. Air can slowly rise along the flow guide plate, the rising speed of bubbles is reduced, the rising path is prolonged, the rising time is prolonged, meanwhile, the bubbles are limited to flow under the flow guide sheets, impact on euglena can be avoided, the intensity, direction and distribution of airflow can be accurately controlled, and the method is very helpful for the fermentation process needing careful regulation and control on fermentation conditions.

Selenium-rich spirulina platensis powder and preparation method thereof

Resumen de: CN118021855A

The invention provides selenium-rich spirulina platensis powder and a preparation method thereof, and belongs to the technical field of health food. The preparation method of the selenium-rich spirulina platensis powder provided by the invention comprises the following steps: preparing a selenium-rich culture solution which is prepared from Zarrouk and Tian lake thermal spring water according to a volume ratio of (1-10): (5-10); adjusting the pH value of the culture solution to obtain a stable culture solution; inoculating spirulina platensis into the stable culture solution in the step (2), and culturing; and centrifuging the spirulina platensis cultured in the step (3) to obtain microalgae mud, and drying to obtain the selenium-rich spirulina platensis powder. According to the method, the microalgae is used for enriching the selenium element in the natural mineral water, and the situation that the selenium element is partially left in the environment to cause environmental pollution is avoided while the selenium element is enriched.

Genetic engineering strain for producing polylactic acid and method for producing polylactic acid

Resumen de: CN118043467A

The invention provides a genetic engineering strain for producing polylactic acid and a method for producing polylactic acid, and the method comprises the following steps: synthesizing a D-lactic dehydrogenase gene, a propionyl-CoA transferase gene and a polyhydroxyalkanoate synthase gene into a target gene, carrying out double enzyme digestion on the target gene and a carrier, purifying, and converting competent cells to obtain a recombinant plasmid; extracting DNA (deoxyribonucleic acid) of the recombinant plasmid, introducing the DNA into an engineering strain, and extracting and verifying to obtain an engineering cell strain; then culturing in a culture medium, introducing carbon dioxide and light into the culture medium, centrifuging, drying and recycling. Natural cyanobacteria are modified, and meanwhile, a high-density fermentation strategy is adopted, so that the biomass of the cyanobacteria is greatly improved; the invention solves the problem that only sugar-sourced raw materials can be used for producing polylactic acid in the prior art.

Method for treating chemical wastewater

Nº publicación: CN118026418A 14/05/2024

Solicitante:

SINOPEC YANGZI PETROCHEMICAL COMPANY LTD
CHINA PETROLEUM & CHEMICAL CORP
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