Alerta

Duplex fluorescent PCR detection primer probe group for duck salmonella enteritidis and salmonella typhimurium, kit, and use thereof

Absstract of: GB2634693A

A duplex fluorescent PCR detection primer probe group of duck Salmonella enteritidis and Salmonella typhimurium, a kit, and the use thereof. Comparative analysis on the whole genomes of Salmonella enteritidis and Salmonella typhimurium is performed to screen out as identification targets a specific fragment Sdf I of lyg D gene of Salmonella enteritidis and a specific gene STM4497 of Salmonella typhimurium, and the present application designs specific primers and probes.

Application of fangchinoline in prevention and treatment of salmonella infection

Absstract of: CN119818497A

The invention discloses an application of fangchinoline in prevention and treatment of salmonella infection. The fangchinoline alkali is an isoquinoline alkaloid, and is a host-oriented treatment natural compound. The fangchinoline disclosed by the invention has a relatively strong effect of treating salmonella infection diseases in vivo and in vitro, and specifically, the fangchinoline can effectively reduce the quantity of intracellular infection bacteria. Administration of the fangchinoline can significantly reduce the load of pathogenic bacteria in the liver, spleen, colon, cecum and ileum of a mouse, and reduce the proinflammatory factor level of an organism. In-vivo and in-vitro safety shows that the fangchinoline has no toxic or side effect below the test dosage. Besides, the fangchinoline alkali and the fluoroquinolone medicine are combined for use, so that the remarkable synergistic antibacterial activity is achieved, and the remarkable synergistic antibacterial activity is shown in reducing the number of salmonella infection bacteria in cells and reducing the load of pathogenic bacteria in the liver, the spleen and the colon of a mouse. The invention provides a new perspective for the development of novel anti-infection drugs, and also provides a new idea for the prevention and control of increasingly serious salmonella infection.

Transaminase mutant and application thereof in asymmetric synthesis of L-glufosinate-ammonium

Absstract of: CN119823960A

The invention discloses a transaminase mutant and application of the transaminase mutant in asymmetric synthesis of L-glufosinate-ammonium. The transaminase mutant is obtained by carrying out single-point saturated mutation or iterative saturated mutation on 13th, 17th, 19th or 22th amino acids of an amino acid sequence shown as SEQ ID NO.1. The invention further discloses a preparation method of the transaminase mutant. The invention provides a novel high-activity transaminase mutant derived from salmonella, and the whole-cell relative enzyme activity of different mutants reaches 250.1%-397.9% compared with that before mutation.

Hippophae rhamnoides seed meal antibacterial peptide as well as preparation method and application thereof

Absstract of: CN119823220A

The invention relates to the technical field of antibacterial peptide preparation, and particularly discloses a hippophae rhamnoides seed meal antibacterial peptide and a preparation method and application thereof.The hippophae rhamnoides seed meal antibacterial peptide is prepared through the steps that hippophae rhamnoides seed meal is smashed, sieved, degreased, dissolved and centrifuged to obtain hippophae rhamnoides seed meal crude protein, then protease is used for enzymolysis, and the hippophae rhamnoides seed meal crude protein is obtained; centrifuging, taking supernatant, and freeze-drying to obtain the sea-buckthorn seed meal antibacterial peptide; the protease is selected from any one of trypsin, alpha-chymotrypsin and alkaline protease. The hippophae rhamnoides seed meal antibacterial peptide prepared by the invention can inhibit staphylococcus aureus, escherichia coli and salmonella.

Quadruple digital PCR (Polymerase Chain Reaction) detection kit for simultaneously detecting four intestinal bacterial pathogens in sewage and application of quadruple digital PCR detection kit

Absstract of: CN119824115A

The invention discloses a quadruple digital PCR (Polymerase Chain Reaction) detection kit for simultaneously detecting four intestinal bacterial pathogens in sewage and application of the quadruple digital PCR detection kit. The invention relates to a quadruple digital PCR (Polymerase Chain Reaction) detection kit, which comprises primers and probes, wherein the primers and the probes are respectively used for detecting salmonella spp., shigella spp., vibrio cholerae and vibrio parahaemolyticus, and the primers and the probes are respectively used for detecting salmonella spp., shigella spp., vibrio cholerae and vibrio parahaemolyticus. Furthermore, the invention also provides an application of the quadruple digital PCR detection kit in preparation of a reagent for simultaneously detecting salmonella, shigella, vibrio parahaemolyticus and vibrio cholerae. When the kit is used for detecting four intestinal bacterial pathogens in sewage, the detection flux is greatly improved, a technical means is provided for efficient development of large-scale sewage monitoring projects, and potential disease outbreak risks can be accurately recognized in the early stage.

Rapid diagnosing Composition for detecting multiple Salmonella enterica serovars

Absstract of: KR20250049691A

본 발명은 살모넬라 혈청형 동시 검출을 위한 신속 진단 조성물에 관한 것으로서, 본 발명에서는 인간에서 발병률이 높은 여러 살모넬라 엔테리카(Salmonella enterica) 혈청형을 진단하기 위한 신속 도트-블롯 면역분석법을 개발하였다. 10개의 상업용 항체(4개의 다클론 항체 및 6개의 단클론 항체)에 대한 적용 가능성을 2016년 미국에서 67.5%의 살모넬라 감염 사례와 관련된 18개의 혈청형을 사용하여 시험하였다. Ab 3(다클론; 18개 혈청형 중 8개), Ab 8(단클론; 18개 혈청형 중 13개) 및 Ab 9(단클론; 18개 혈청형 중 10개) 항체는 웨스턴 블롯에서 높은 검출율을 나타냈고, 두 항체의 조합(Ab 3+8, Ab 3+9 및 Ab 8+9)을 도트-블롯 분석에 적용하였다. Ab 3+8의 조합은 시험된 18개의 혈청형 중 15개를 식별할 수 있었는데, 이는 S. Enteritidis, S. Typhimurium, S. Javiana, S. I 4,5,12:i:-, S. Infantis, S. Montevideo, S. Braenderup, S. Thompson, S. Saintpaul, S. Heidelberg, S. Oranienburg, S. Bareilly, S. Berta, S. Agona 및 S. Anatum을 포함하며, 2016년 미국에서 53.7%의 살모넬라 감염 사례와 관련되어 있다. 본 발명에서 최적화한 면역분석법은 3시간 안에 주요 15종의 혈청형을 진단할 수 있고, 이러한 비용 효율적이고 신속한 방법은 살모넬라 검출을 위한 현장 비색 방법으로 유용하게 활용될 수 있을 것으�

Phage composition for preventing and treating salmonella abortus of horses, biological preparation and application of bacteriophage composition and biological preparation

Absstract of: CN119799656A

The invention belongs to the technical field of biology, and particularly relates to a bacteriophage composition for preventing and treating horse salmonella abortus, a biological preparation and application of the bacteriophage composition and the biological preparation. The bacteriophage composition comprises a bacteriophage 4FS1, a bacteriophage DS-BP2 and a bacteriophage DS2; the preservation number of the bacteriophage 4FS1 is CCTCC (China Center For Type Culture Collection) NO: M 20242020; the preservation number of the bacteriophage DS-BP2 is CCTCC (China Center For Type Culture Collection) NO: M 202402021; the preservation number of the bacteriophage DS2 is CGMCC (China General Microbiological Culture Collection Center Three kinds of virulent bacteriophages 4FS1, DS-BP2 and DS2 aiming at different host spectrums of salmonella are separated and screened, the three kinds of virulent bacteriophages 4FS1, DS-BP2 and DS2 are combined into the bacteriophage cocktail, the occurrence of bacteriophage-tolerant strains can be effectively prevented or delayed, and the screened bacteriophages have a synergistic interaction effect; the three bacteriophages obtained by separation can be independently used or combined into a bacteriophage cocktail for preventing or treating infection caused by horse abortion salmonella, such as female donkey abortion and the like.

Primer composition and kit for detecting infectious diarrhea pathogens

Absstract of: CN119799975A

The invention provides a primer composition and a kit for detecting infectious diarrhea type pathogens. Staphylococcus aureus, escherichia coli, klebsiella pneumoniae, proteus mirabilis, listeria monocytogenes, pseudomonas aeruginosa, salmonella enteritidis, clamping film bacillus aerogenes, vibrio cholerae, clostridium difficile, aeromonas capora, yersinia enterocolitica, plesiomonas shigelloides, vibrio parahaemolyticus, campylobacter jejuni, candida albicans and aspergillus fumigatus are detected at the same time. The kit can be one or any combination of Aspergillus flavus, entamoeba histolytica, human adenovirus A, human adenovirus B, human adenovirus C, human adenovirus F, human bocavirus, rotavirus, norovirus, fiveleaf virus and human astrovirus, and has the advantages of short time consumption, high resolution, high flux, high sensitivity and the like.

SipD重组蛋白在制备检测禽沙门氏菌抗体的间接ELISA检测试剂盒中的应用

Absstract of: CN119804862A

本发明涉及血清学检测技术领域，特别是涉及SipD重组蛋白在制备检测禽沙门氏菌抗体的间接ELISA检测试剂盒中的应用。本发明通过大量研究，成功筛选出用于检测禽沙门氏菌抗体的特异性抗原‑SipD蛋白。本发明以禽沙门氏菌III型分泌系统SipD亚基(SipD蛋白)为抗原，经蛋白表达纯化后获得包被抗原(SipD重组蛋白)，通过反应条件的摸索与优化，最终建立了灵敏性高、特异性强和重复性好的禽沙门氏菌间接ELISA检测方法。相比于传统的玻片凝集抗原检测方法，该方法极大地提高了检测通量和检测效率。该检测方法的建立对于人类、动物、食品中的沙门氏菌检测具有重要的公共卫生意义。

Eubacterium mucilaginosus E11405 and application thereof in bacteriostasis and anti-inflammation

Absstract of: CN119799564A

The invention relates to the technical field of microorganisms, in particular to Eubacterium mucilaginosus E11405 and application thereof in bacteriostasis and anti-inflammation. The preservation number of the mucus eubacterium is CGMCC (China General Microbiological Culture Collection Center) No.31231. The application comprises inhibition of microorganisms and anti-inflammation. The microorganisms comprise one or more of listeria monocytogenes, staphylococcus aureus, salmonella typhimurium or escherichia coli. The Eubacterium mucilaginosus provided by the invention can relieve various infection symptoms caused by salmonella infection, and shows the potential in preparation of drugs for treating and/or preventing infection of pathogenic bacteria such as salmonella. In addition, the Eubacterium mucilaginosus provided by the invention can also effectively relieve inflammatory bowel diseases and relieve intestinal mucosa injury and colitis symptoms, provides a new strategy for preventing and treating inflammatory bowel diseases, and has potential clinical application prospects.

Traditional Chinese medicine composite lactobacillus acidophilus NM probiotic preparation for improving oral bacterial flora and preparation process of traditional Chinese medicine composite lactobacillus acidophilus NM probiotic preparation

Absstract of: CN119792444A

The invention discloses a traditional Chinese medicine composite lactobacillus acidophilus NM probiotic preparation for improving oral bacterial flora and a preparation process thereof, and relates to the related field of biotechnology. According to the traditional Chinese medicine composite lactobacillus acidophilus NM probiotic preparation, the synergistic effect of probiotics and traditional Chinese medicine is combined, the traditional Chinese medicine composite lactobacillus acidophilus NM probiotic preparation has the multiple advantages of regulating oral flora, resisting bacteria and diminishing inflammation, resisting oxidation, relieving fatigue and the like, lactobacillus acidophilus NM can tolerate acid and cholate, pathogenic bacteria such as escherichia coli, staphylococcus aureus and salmonella are effectively inhibited, and the traditional Chinese medicine composite lactobacillus acidophilus NM probiotic preparation is suitable for oral administration. The traditional Chinese medicine composition has the advantages that the traditional Chinese medicine composition can be used for reducing dental caries, gingivitis and ozostomia, is free of antibiotic drug resistance and is safer after being used for a long time, radix scutellariae, honeysuckle and green tea extracts are rich in flavonoids and tea polyphenols and can further resist bacteria and inflammation and improve oral health, and meanwhile, the probiotics can reduce blood lactic acid, blood urea nitrogen

Fusion protein of salmonella typhimurium flagellin derivative and Seneca virus antigen and application thereof

Absstract of: CN119798461A

The invention belongs to the technical field of biology, and discloses a fusion protein of a salmonella typhimurium flagellin derivative and a Seneca virus antigen and application of the fusion protein. A salmonella typhimurium flagellin derivative gene and a Seneca virus antigen gene are fused, codon optimization is carried out, the fusion protein is cloned to a pET-28a (+) vector, an escherichia coli system is utilized to express the fusion protein, and in-vivo experiments prove that the fusion protein shows good biological activity, can activate the activity of a TLR5 ligand and promote the activation of macrophages, and can be used for preparing a recombinant vector. And a foundation is laid for the development of Seneca virus subunit vaccines.

一种减毒沙门氏菌来源的细菌外膜囊泡蛋白载药系统及其制备方法和应用

Absstract of: CN119792228A

本发明公开了一种减毒沙门氏菌来源的细菌外膜囊泡蛋白载药系统及其制备方法和应用，本发明利用细菌外膜囊泡蛋白载药系统可以在细菌外膜囊泡表面实现蛋白药物的“即插即用”。本发明构建了细菌外膜囊泡蛋白载药系统。本发明敲除了减毒鼠伤寒沙门氏菌VNP20009的OmpA，并回补在胞外环结构域中插入SpyTag的重组OmpA，利用SpyTag/SpyCatcher间自发形成异肽键的性质，将SpyCatcher与蛋白药物的融合蛋白展示在细菌外膜囊泡表面，制备方法简单，容易操作，在细菌外膜囊泡表面展示多种蛋白，并高效保护和负载蛋白药物。结合细菌外膜囊泡的免疫刺激特性，实现肿瘤的高效治疗。

Salmonella-resistant nano material screening system

Absstract of: CN119811555A

The invention discloses an anti-salmonella nanometer material screening system. The system comprises the following steps: designing a candidate material structure through molecular dynamics simulation; performing comparative observation on the synthetic material and antibiotics, and collecting drug sensitivity test images to form a data set; training a computer vision model to automatically distinguish the antibacterial effect; carrying out high-throughput automatic testing on the candidate material by using a microfluidic platform; efficient screening is carried out according to the test effect; the system can realize automatic mining of the anti-salmonella nanometer material.

Phytobacterium plantarum with bacteriostatic effect and application thereof

Absstract of: CN119799598A

The invention discloses a plant lactobacillus with a bacteriostatic effect and application of the plant lactobacillus. The lactobacillus plantarum is named as lactobacillus plantarum C07, the preservation number is CCTCC NO: M20242086, the preservation unit is China Center for Type Culture Collection, the preservation time is September 26, 2024, and the preservation address is Wuhan University, Wuhan University, No. 299, eight road, Wuchang District, Wuhan City, Hubei Province. The strain can produce lactic acid and indole-3-lactic acid at high yield, has a very strong antibacterial effect on multiple putrefying and pathogenic microorganisms such as staphylococcus aureus, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella and mould, and is a broad-spectrum antibacterial microorganism.

IMPROVED SALMONELLA VECTORED THERAPIES FOR TREATMENT OF CANCER

Absstract of: WO2023235633A2

A genetically modified Salmonella cell (GMSC) engineered to exhibit specific targeting to cells and regulated delayed lysis in vivo, the GMSC comprising a first heterologous nucleic acid that encodes a first gene product that causes the GMSC to be selectively localized to and/or internalized by a target cell in vivo and a second heterologous nucleic acid that encodes a second gene product that facilitates killing of the target cells following internalization.

METHOD FOR IDENTIFYING ESBL ENZYMES IN ENTEROBACTERIA

Absstract of: WO2023227848A1

The present invention relates to a method for determining the properties of resistance to beta-lactam antibiotics of a strain of enterobacteria present in a biological sample, which method comprises the steps of: a) lysis of the bacteria and hydrolysis of the bacterial proteins in order to obtain a mixture of peptides, b) analysis of this mixture of peptides by targeted mass spectrometry coupled with reverse phase liquid chromatography, detection of at least one specific peptide of an enzyme exhibiting extended spectrum beta-lactamase (ESBL) activity being indicative of the resistance to beta-lactams of the strain of enterobacteria present in the biological sample, the method being characterised in that the analysis step (b) is carried out in the presence of an ion pairing reagent (IPR) added into the chromatography column or into the mixture of peptides.

Antibacterial peptide CL18 and application thereof

Absstract of: CN119775364A

The invention discloses an antibacterial peptide CL18 and application thereof, and the amino acid sequence of the antibacterial peptide CL18 is shown as SEQ ID NO.1. The antibacterial peptide disclosed by the invention is strong in antibacterial ability, and can play a role in bacteriostasis at a relatively small concentration. In addition, the antibacterial peptide provided by the invention is not influenced by the drug resistance of salmonella, has an obvious inhibition effect on salmonella, and can effectively prevent and control salmonellosis. The antibacterial peptide disclosed by the invention is prepared into an antibacterial drug, a feed additive or a feeding material, and has important significance on healthy development of livestock and poultry breeding industry.

Bacillus velezensis GY1 and application thereof

Absstract of: CN119776182A

The invention discloses bacillus velezensis GY1, which is preserved in the Guangdong Microbial Culture Collection Center at June 28, 2024, and the preservation number is GDMCC No: 64792. The bacillus velezensis GY1 has the advantages that the bacillus velezensis GY1 is named as GDMCC NO: 64792, and the bacillus velezensis GY1 is named as GDMCC NO: 64792; the bacillus velezensis GY1 can degrade 100% of nitrogen and 56.4% of nitrogen in an M9 culture medium containing 200 mg/L of ammoniacal nitrogen and 1000 mg/L of ammoniacal nitrogen respectively, and the ammonia reduction rate of the bacillus velezensis GY1 in actually produced culture water reaches 53.3%. The compound has a relatively strong inhibition effect on common pathogenic bacteria on aquatic products, such as vibrio alginolyticus, salmonella and vibrio parahaemolyticus.

一种感应铁镁离子的细菌传感器的构建方法及其应用

Absstract of: CN119776251A

本发明公开了一种感应铁镁离子的细菌传感器的构建方法及其应用。本发明属于微生物领域，具体涉及一种感应铁镁离子的细菌传感器的构建方法及其应用。重组大肠杆菌含有超折叠绿色荧光蛋白sfGFP编码基因、感应蛋白PmrB突变体编码基因和响应调节蛋白PmrA突变体编码基因、信号传导蛋白PmrD编码基因和强核糖体结合位点RBS，并含有通过调节蛋白PmrA激活的磷酸化酶PmrG的启动子驱动的T7 RNA聚合酶编码基因和T7启动子驱动的sfGFP编码基因，同时过表达磷酸乙醇胺转移酶EptA编码基因。本发明构建了检测铁离子浓度的细菌传感器，并可通过铁镁离子的诱导改变细菌传感器的脂多糖的修饰，增强细菌的耐药性。

Composite plant essential oil and emulsion for antagonizing bacterial biofilm as well as preparation method and application of composite plant essential oil and emulsion

Nº publicación: CN119770542A 08/04/2025

Applicant:

GUANGXI UNIV OF CHINESE MEDICINE
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Absstract of: CN119770542A

The invention belongs to the technical field of biology, and particularly discloses a composite plant essential oil for antagonizing a bacterial biofilm, which is composed of cinnamon essential oil and star anise essential oil. The compound plant essential oil disclosed by the invention consists of the cinnamon essential oil and the star anise essential oil which have a synergistic effect, have good bacteriostatic activity on salmonella, escherichia coli and the like, can inhibit the formation of a bacterial biofilm, and have a strong removal effect on the formed bacterial biofilm; and when being used for removing bacterial biofilms, the cleaning agent has the characteristics of good removal effect, low toxicity and the like.