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102
results.
Updated on
21/08/2025 [07:11:00]
Results 75 to 100 of 102
Absstract of: CN120082456A
The invention provides bacillus coagulans and application thereof, relates to the technical field of microorganisms, and aims to solve the problem that a biological material with a strong antibacterial function and good tolerance is lacked in the prior art. The invention provides bacillus coagulans XH-2, the preservation number of the bacillus coagulans XH-2 is CCTCC NO: M 2023759, the bacillus coagulans XH-2 is preserved in the China Center for Type Culture Collection on May 15, 2023, and the preservation address is Wuhan University, China. The bacillus coagulans XH-2 disclosed by the invention has a relatively strong inhibition effect on escherichia coli, and also has a relatively good inhibition effect on salmonella gallinarum, salmonella suis and salmonella typhimurium at the same time. According to the invention, the bacillus coagulans XH-2 is used as an active ingredient to prepare the livestock or poultry feed additive, and the strain has strong gastric juice and cholate tolerance, and can inhibit the growth of pathogenic bacteria and improve the conversion and utilization of animal feed.
Absstract of: CN120060515A
The invention discloses an RPA primer group and a kit for rapidly detecting salmonella pullorum based on RPA-CRISPR/Cas12a, and belongs to the technical field of salmonella pullorum visual rapid detection. A specific target gene ipaj in a salmonella pullorum genome is taken as a target spot, an RPA primer pair and a specific crRNA sequence are designed and optimized, the salmonella pullorum is detected by combining an RPA isothermal amplification method and a CRISPR/Cas12a detection method, a detection result can be interpreted by naked eyes under blue light, and the detection result is accurate. The kit has the characteristics of high sensitivity, strong specificity, accurate result, short time consumption, simplicity and convenience in operation and high practicability, and is suitable for rapid screening or detection of the pullorum disease on site.
Absstract of: CN120064644A
The invention discloses a high-specificity pullorum disease salmonella gallinarum plate agglutination antigen preparation process, which comprises the following steps: respectively resuscitating pullorum disease salmonella C79-1 and C79-7 strains to prepare seed solutions, inoculating a suitable culture medium, carrying out fermentation culture according to a specific process, carrying out centrifugal collection on thalli after inactivation, and carrying out freeze drying to obtain the high-specificity pullorum disease salmonella gallinarum plate agglutination antigen. The preparation method comprises the following steps: carrying out high pressure at 121 DEG C for 2 hours, washing for multiple times, removing protein antigen components which are easily subjected to cross reaction with escherichia coli or enterobacteriaceae bacteria which are commonly used in other environments, adjusting the C79-1 strain and the C79-7 strain to proper bacterium concentration, adding Tween-20, crystal violet and glycerol, and fully homogenizing to obtain the salmonella typhimurium plate agglutination antigen for pullorum disease. The antigen has higher specificity, and is more accurate when being used for purifying and detecting the pullorum disease; according to the fermentation process, the bacterial yield is 4 times of that of common fermentation and 2 times of that of solid culture, ethanol precipitation is not needed, and the production cost is lower; tween-20 is added, so that the surfa
Absstract of: CN120058920A
The invention discloses an anti-S11 type riemerella anatipestifer monoclonal antibody as well as a blocking ELISA antibody detection kit and application thereof, and belongs to the technical field of poultry immunology. The monoclonal antibody provided by the invention comprises a heavy chain and a light chain, the heavy chain comprises VHCDR1, VHCDR2 and VHCDR3 of which the amino acid sequences are as shown in SEQ ID NO: 1-3, and the light chain comprises VLCDR1 of which the amino acid sequence is as shown in SEQ ID NO: 4, VLCDR2 of which the amino acid sequence is LVS and VLCDR3 of which the amino acid sequence is as shown in SEQ ID NO: 5; the blocking ELISA antibody detection kit prepared from the monoclonal antibody can specifically detect the level of a riemerella anatipestifer serotype 11 (S11 type) antibody in a sample, has no cross reactivity with riemerella anatipestifer S1 type, riemerella anatipestifer S2 type, riemerella anatipestifer S5 type, riemerella anatipestifer S6 type and riemerella anatipestifer S14 type, avian pathogenic escherichia coli, avian pasteurella multocida and salmonella, and is high in sensitivity and specificity.
Absstract of: CN120053424A
The invention belongs to the technical field of anti-infection drugs, and particularly relates to medical application of apigenin in preparation of a salmonella flagellum inhibitor. A migration inhibition test (0.3% agar), minimum inhibitory concentration (MIC) determination, a Caco-2 cell adhesion test and a molecular docking test prove that the apigenin can specifically inhibit salmonella flagellum-mediated migration ability (inhibition rate gt; 49%) and host cell adhesion capability (reduced by more than or equal to 58%), and does not affect bacterial growth (pgt; 0.05), the apigenin can be used for targeting flagellin FljB, FliC, FlgN, FlgA and FlgG (binding energy per lt; -5kcal/mol) can block flagellar movement, and has an application prospect in prevention and control of livestock salmonella related diseases.
Absstract of: CN120060052A
The invention discloses a strain of enterococcus faecium HKS023 and application of the enterococcus faecium HKS023. The enterococcus faecium HKS023 is preserved in China General Microbiological Culture Collection Center on February 14, 2023, and the preservation number of the enterococcus faecium HKS023 is CGMCC No.26552. The enterococcus faecium HKS023 is named as enterococcus faecium HKS023. According to the invention, a new strain of enterococcus faecium HKS023 is screened from fresh feces of healthy weaned piglets, corn flour is taken as a raw material, L-lactic acid is produced through fermentation, the acid yield is high, and the enterococcus faecium HKS023 has a very strong inhibition effect on the growth of salmonella and can inhibit the growth of escherichia coli and staphylococcus aureus to a certain extent. The enterococcus faecium HKS023 microbial feed additive is produced by effectively combining and fermenting common raw materials including calcium carbonate, beef extract, yeast extract, peptone, glucose, sodium acetate, dipotassium phosphate, magnesium sulfate, manganese sulfate and the like, the growth performance of livestock and poultry can be effectively improved, the diarrhea rate is reduced, the immunity of the livestock and poultry is improved, and the economic and social benefits are huge.
Absstract of: CN120060164A
The invention relates to the technical field of microorganisms, in particular to a salmonella manhattan phage and application thereof. The phage is named as vB Sal M467 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.46195, and the preservation date is September 29, 2024. The bacteriophage provided by the invention is separated from sewage of a farm and belongs to a tailed virus, and genomics shows that the bacteriophage belongs to a Kayfunavius genus, a non-virulence gene and a drug-resistant gene, and has safety. The biological characteristic result of the bacteriophage shows that at least eight salmonella serotypes can be split, the bacteriophage has good stability to temperature (-20 DEG C to 60 DEG C) and acid-base property (pH = 3-12), the outbreak amount is 158.83 PFU/cell, and the bacteriophage can be massively proliferated in a short time. The bacteriophage can be used for preventing and controlling salmonella in food such as lettuce and milk.
Absstract of: CN120052369A
The invention discloses a preparation method of a fresh fruit and vegetable surface cleaning disinfectant and application of the fresh fruit and vegetable surface cleaning disinfectant in fruit and vegetable cleaning.The preparation method comprises the steps that firstly, the antibacterial effect of citric acid, tartaric acid and sodium hypochlorite is evaluated, the synergistic antibacterial effect of different compounding modes is optimized, and meanwhile the optimal compounding formula is screened out; and the antibacterial activity is verified by using a time-sterilization curve. The compound disinfectant has an obvious inhibition effect on typical food-borne pathogenic bacteria such as salmonella typhimurium and escherichia coli O157: H7, and meanwhile, in fruit and vegetable cleaning application, the effectiveness of the synergistic antibacterial effect of the method is evaluated under a cherry tomato and lettuce system; and the molecular mechanism of sterilization is explored from the aspects of cell membrane morphology and property change at the cellular level, and the synergistic effect mechanism of citric acid and sodium hypochlorite is discussed. The method can be applied to fresh fruit and vegetable surface cleaning and disinfection, and an important theoretical basis is provided for guaranteeing the safety of fresh fruit and vegetable microorganisms.
Absstract of: CN120060507A
The invention discloses a multi-pathogen visual detection method based on top-speed PCR (Polymerase Chain Reaction), and belongs to the technical field of molecular biological detection. The invention designs specific primers and molecular beacons of salmonella Sa, vibrio parahaemolyticus VP and infectious subcutaneous and hematopoietic necrosis virus IHHNV for multiple PCR (Polymerase Chain Reaction), and three pathogens can be simultaneously amplified in a PCR system. In addition, the molecular beacon and the target are combined to expose the fluorophore, and the three pathogens can be distinguished without opening a cover by means of a self-made visual device. The multiple visual PCR detection method provided by the invention has the advantages of strong specificity, high sensitivity, short detection time, direct observation and the like, can realize simultaneous detection of multiple pathogens in a single sample, saves the cost, has higher economic value, and is suitable for on-site instant detection.
Absstract of: CN120060163A
The invention discloses an Escherichia coli bacteriophage PEC-36 with cross-species host characteristics and application of the Escherichia coli bacteriophage PEC-36, and belongs to the technical field of biology. The Escherichia coli bacteriophage PEC-36 is separated from sewage of a pig factory, can simultaneously split O8: H7, O21: H34, O23: H16, O25: H4, O81: H27 and H25 serotype strains of Escherichia coli and fowl typhoid, London, swine cholera and other serotype strains of salmonella, and has a cross-species splitting characteristic. Experiments prove that the bacteriophage has the advantages of high titer, high stability, high safety and the like, and can remarkably inhibit the growth of escherichia coli and salmonella, so that a technical support is provided for research and development of bacteriophage preparations for treating infection related to escherichia coli and salmonella; the invention provides a safe and efficient antibiotic alternative scheme for treating human and animal Escherichia coli and salmonella related infection, and has a good application prospect.
Absstract of: CN120053379A
Construction and application of an intratumoral self-proliferation enhanced oncolytic microbial delivery system belong to the technical field of pharmaceutical preparations, a hydrogen sulfide donor is endowed with a membrane-insertable regional structure through a synthetic reaction, then a lipid membrane containing the membrane-insertable hydrogen sulfide donor is coated with bacteria through a self-assembly effect, and the membrane-insertable hydrogen sulfide donor is obtained. An oncolytic microbial delivery system with enhanced intratumoral self-proliferation is prepared. After the delivery system is intravenously injected, the delivery system is enriched in a tumor in a targeted manner, hydrogen sulfide released by a donor can be converted into tetrathionate in a tumor inflammation microenvironment, tetrathionate respiration belongs to specific respiration of salmonella, and the specific respiration belongs to specific respiration of salmonella. The proliferation of a salmonella tumor part is specifically promoted through two respiratory chains of aerobic respiration and tetrasulfate respiration, so that the pyroptosis of tumor cells is enhanced, and the anti-tumor purpose is achieved. The invention provides a brand new theory and an advanced solution for cancer treatment, also provides a promising strategy and exploration thought for clinical application of attenuated salmonella, and has important scientific research value.
Absstract of: NZ752690A
An extract of an herbal composition comprising at least two different dried plants useful as antimicrobial and/or antibiofilm agent in the treatment or prevention of microbial infections caused by bacteria, such as for example Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Streptococcus or Staphylococcus, or by fungi, such as for example is herein described. It has been found that in such extract, the active ingredients exert their biological effects in a synergistic manner. The extract may constitute the active ingredient of a food supplement, a nutraceutical, pharmaceutical or cosmetic composition or a functional food or a food additive. A process for preparing said extract is also described here.
Absstract of: CN120060007A
The invention relates to the technical field of microorganisms, in particular to clostridium butyricum CB24011205 capable of inhibiting growth and colonization of vibrio cholerae and application of the clostridium butyricum CB24011205. According to the present invention, the clostridium butyricum CB24011205 strain is separated from the intestinal tract content of the marine white shrimp, and the preservation number of the clostridium butyricum CB24011205 strain is CGMCC No. 33030; the strain has the capability of efficiently inhibiting vibrio cholerae, can be compounded with daidzein to prepare a microecological preparation, not only has remarkable advantages in colonization and growth of vibrio cholerae, but also can provide certain protection for other pathogenic bacteria (such as escherichia coli and salmonella), can be used for enhancing intestinal immunity and repairing functions, and can be used for preparing the microecological preparation. Good application prospects are realized.
Absstract of: US2025169515A1
A continuous feed, shell egg pasteurization system and method uses sprayed heated water. The unpasteurized shell eggs are conveyed and rotated through a horizontal tunnel. An overlapping pattern of heated water is sprayed downward onto the rotating shells eggs. Verified testing shows that the shell eggs achieve a statistical 5 log reduction of Salmonella enteritidis that may have been present in the yolk in the respective unpasteurized shell egg, while at the same time are not overcooked and have little to no whitening of the albumen.
Absstract of: US2025170230A1
The subject relates to an isolated antibody that specifically binds to O25b antigen of multi drug resistant (MDR) E. coli strains, its medical and diagnostic use, method of producing the antibody, including an isolated nucleotide sequence, plasmids and host cells as used in the production of the antibody; and further an isolated epitope recognized the specific antibody.
Absstract of: AU2023361317A1
The present invention relates to a modified live attenuated Gram-negative bacteria, wherein said bacteria has been modified in such a way that RNA molecules can be safely and efficiently delivered to target eukaryotic cells. As such, the present invention relates a to a bacterial delivery system and various uses and methods thereof.
Absstract of: CN120051271A
The present invention provides a method of treating an infectious disease. The method comprises the step of administering to a subject in need thereof an effective amount of (i) a polymer-flavonoid conjugate, (ii) a flavonoid oligomer, or (iii) a micelle having a shell formed from one or more polymer-flavonoid conjugates or one or more flavonoid oligomers, or a combination thereof and having an agent encapsulated within the shell. The methods of the present invention can effectively treat viral infections, such as, for example; severe acute respiratory syndrome coronavirus (SARS-CoV), enterovirus, HIV, hepatitis B virus, MERS-CoV, influenza virus, dengue fever virus, respiratory syncytial virus, hepatitis C virus, monkey pox virus, human papilloma virus, methicillin-resistant staphylococcus aureus, pseudomonas, tuberculosis, bacillus anthracis, tetanus, streptococcus pneumoniae, meningococcus, escherichia coli, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, , Legionella genus, Neisseria gonorrhoeae, Neisseria meningitidis, and Salmonella genus.
Absstract of: CN120040584A
The invention discloses a monoclonal antibody CJ116 for resisting pathogenic campylobacter and an application of the monoclonal antibody CJ116. In the monoclonal antibody CJ116, the amino acid sequence of a heavy chain variable region is as shown in SEQ ID NO.1, and the amino acid sequence of a light chain variable region is as shown in SEQ ID NO.5. The CJ116 monoclonal antibody has high affinity and high specificity, the detection sensitivity of the CJ116 monoclonal antibody to campylobacter jejuni and campylobacter colons can reach 10 < 2 > CFU/mL, and the detection sensitivity of the CJ116 monoclonal antibody to campylobacter fetus, campylobacter upsackii and campylobacter seagull is 10 < 3 > CFU/mL. However, the enteropathogenic bacteria have no cross reaction with other common enteropathogenic bacteria such as pathogenic Escherichia coli, salmonella enteritidis, yersinia enterocolitica, Shigella flexneri, Shigella baumannii and Staphylococcus aureus.
Absstract of: CN120041399A
The invention discloses a wide-splitting-spectrum salmonella typhimurium bacteriophage, a bacteriophage composition and application thereof, the bacteriophage is named as salmonella bacteriophage PC817 which is preserved in China General Microbiological Culture Collection Center on August 16, 2024, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.9687. The invention further discloses a preparation method of the wide-splitting-spectrum salmonella typhimurium bacteriophage. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection The bacteriophage PC817 is a salmonella typhimurium bacteriophage with a wide lysis spectrum, has the characteristic of high lysis rate for different types of salmonella, and can be used for preparing drugs for treating or preventing salmonellosis, environment disinfectants, feed additives, drinking water additives, food preservatives, detection kits and the like. While salmonella typhimurium infection is solved, the problems of antibiotic residues and pathogen drug resistance caused by use of antibiotics are avoided. The bacteriophage and the composition thereof can provide a more effective scheme for prevention and control of pigeon salmonellosis.
Absstract of: CN120041351A
The invention discloses enterococcus faecium capable of degrading three mycotoxins at the same time and application of the enterococcus faecium, and belongs to the technical field of microorganisms. The enterococcus faecium JBHBIO-BSC1 disclosed by the invention is preserved in the China Center for Type Culture Collection, and the preservation number of the enterococcus faecium JBHBIO-BSC1 is CCTCC (China Center for Type Culture Collection) NO: M 20241918. The enterococcus faecium disclosed by the invention has the most remarkable characteristic that the enterococcus faecium has an obvious degradation effect on the aflatoxin, the vomitoxin and the zearalenone, and the degradation rates of the aflatoxin, the vomitoxin and the zearalenone can respectively reach 74.67%, 85.83% and 82.12%. The enterococcus faecium disclosed by the invention is natural, safe, acid-resistant, alkali-resistant and high-temperature-resistant, and has relatively strong organic acid production capacity. In addition, the strain has an obvious inhibition effect on escherichia coli, salmonella and staphylococcus aureus, and is expected to be applied to the fields of food processing and microbial pharmacy.
Absstract of: KR20250073901A
본 발명은 혈액 기반 인체 마이크로바이오타 조성 검사 방법 및 이의 용도에 관한 것으로, 보다 구체적으로는 환자 유래 단리된 생물학적 시료를 384종의 인체 장내미생물 항원으로 이루어진 마이크로어레이에 처리하는 단계를 포함하는 질환 관련 치료제 후보 장내미생물의 스크리닝 방법 및 상기 스크리닝 방법을 통해 선발된 질환 관련 치료제 후보 장내미생물에 관한 것이다.
Absstract of: US2023406892A1
The present invention relates to bacteriocins for control of Salmonella enterica (salmocins). The bacteriocins are derived from Salmonella. The salmocins can be expressed in plants and can be used in a method of preventing or reducing infection or contamination of an object with Salmonella.
Absstract of: CN120025987A
The invention relates to the field of microorganisms, and provides a bacteriophage, a composition and application of the bacteriophage, and the preservation number of the bacteriophage is CCTCC M 2024569. The invention discloses a bacteriophage capable of splitting multiple salmonella and escherichia coli O157: H7, and a bacteriophage composition comprising the bacteriophage. The effective components of the bacteriophage composition comprise the bacteriophage or the bacteriophage composition. The bacteriophage provided by the invention has good biological characteristics, does not contain virulence genes and antibiotic resistance genes, and is wide in host spectrum and strong in splitting property. The bacteriophage preparation provided by the invention can be used as a biological prevention and control preparation to control infection of salmonella and escherichia coli O157: H7 antibiotic-resistant strains in food.
Absstract of: CN120025401A
The invention provides chitosan coupled polypeptide and a preservative film prepared from the chitosan coupled polypeptide, active peptide used for preparing the preservative film has good antibacterial activity on escherichia coli, salmonella, staphylococcus aureus and drug-resistant escherichia coli, and the amino acid sequence of the active peptide is SEQ ID NO: 1. The preservative film prepared by coupling the active peptide and the chitosan provided by the invention has remarkable antibacterial activity, also has good resistance to drug-resistant strain drug-resistant escherichia coli, is good in mechanical property, air permeability, moisture penetrability and stability, and is suitable for being used in the fields of food preservation, medical dressing and the like.
Nº publicación: CN120025989A 23/05/2025
Applicant:
YANGZHOU UNIV
\u626C\u5DDE\u5927\u5B66
Absstract of: CN120025989A
The invention discloses a salmonella longtail bacteriophage and application thereof, the bacteriophage is salmonella bacteriophage YZA15 and is preserved in China Center for Type Culture Collection, the preservation number is CCTCC NO: M 20242878, the preservation date is December 23, 2024, and the preservation address is Wuhan. The salmonella bacteriophage YZA15 disclosed by the invention is good in stability, has no toxic drug-resistant genes, has splitting activity on main epidemic serotypes (mouse typhoid, enteritis and pullorum disease) of salmonella, is relatively specific in host range, short in incubation period and high in filial generation bacteriophage outbreak amount, can be applied to links of livestock breeding, slaughtering, processing and breeding, and has a broad application prospect. And a safe and efficient biological antibacterial product is provided for livestock and poultry environments and products thereof.
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