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82
results.
Updated on
15/07/2026 [07:55:00]
Results 50 to 75 of 82
Absstract of: MX2025013800A
Disclosed herein are methods and systems for rapid detection of microorganisms in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as Salmonella-specific bacteriophage, allows detection of a specific microorganism, such as Salmonella spp. and an indicator signal may be amplified to optimize assay sensitivity.
Absstract of: CN121950481A
The invention relates to the technical field of biomedicine detection, and discloses a biomedicine-based salmonella detection instrument and method for livestock and poultry breeding, the biomedicine-based salmonella detection instrument comprises a detection table and an electron microscope mounted at the upper end of the middle part of the detection table, a conveying assembly is arranged at the upper end of the detection table, and the conveying assembly is used for continuously feeding a culture dish; and the conveying assembly comprises two groups of rotating rods connected with the detection table. According to the salmonella detection instrument and method for livestock and poultry breeding based on biomedicine, stable power is provided for the conveying assembly through the first motor, and continuous and automatic conveying of culture dishes is achieved in cooperation with transmission of a synchronous belt wheel and a feeding belt; the feeding assembly synchronously completes accurate feeding of single culture dishes through the power of the conveying assembly by means of linkage of a driving belt wheel, a driven belt wheel and a transmission belt, a power source does not need to be additionally arranged, the structure is simplified, and meanwhile cooperation and consistency of the feeding rhythm and the conveying rhythm are achieved; and the detection requirements of large-batch salmonella in large-scale livestock and poultry breeding are effectively met.
Absstract of: CN121943972A
The invention belongs to the technical field of biology, and discloses application, a screening method and a medicine of Brawler's bacteria, and application of the Brawler's bacteria to preparation of anti-inflammatory drugs. Firstly, it is determined that intestinal microorganisms play an important role in resisting salmonella infection through a fecal microorganism transplantation test; secondly, screening is carried out on excrement extracts of chicken infected with salmonella, it is found that the abundance of the Brawler is extremely high, and it is further found through in-vitro tests and in-vivo tests that the Brawler can inhibit the systemic inflammatory response, caused by infection of salmonella enteritidis, of the chicken.
Absstract of: CN121951093A
The invention relates to the technical field of biology, in particular to a rapid detection kit for bacterial food-borne pathogenic microorganisms and application of the rapid detection kit. The invention develops a primer probe combination kit for detecting food-borne bacteria salmonella, staphylococcus aureus, vibrio parahaemolyticus, listeria monocytogenes, escherichia coli O157 and clostridium perfringens through optimization of primers, optimization of hydrogel components and a system and the like. The primer probe combination kit is used for detecting food-borne bacteria salmonella, staphylococcus aureus, vibrio parahaemolyticus, listeria monocytogenes, escherichia coli O157 and clostridium perfringens. The kit is used for detecting salmonella, staphylococcus aureus, vibrio parahaemolyticus, listeria monocytogenes, Escherichia coli O157 and clostridium perfringens. The kit is high in sensitivity and specificity, strong in anti-interference capability, excellent in stability and portability, rapid and efficient in detection, simple and convenient to operate and low in cost, and can realize result visualization and relative quantification, so that the kit has wide application prospects and values.
Absstract of: CN121942903A
The invention discloses application of lactobacillus salivarius with bacteriostatic ability and capable of improving flavor of fermented milk in the fermented milk, and belongs to the technical field of microorganisms. The fermented milk prepared from the lactobacillus salivarius combined CCFM1332 can inhibit porphyromonas gingivalis, has a strong inhibition effect on the forming ability of salmonella typhimurium, staphylococcus aureus, pseudomonas aeruginosa and pseudomonas fluorescens biological membranes, and increases the content of essential amino acids and the content of flavor amino acids in the fermented milk; the proportion of bitter amino acids is reduced and the flavor of the fermented milk is improved. In addition, the CCFM1332 is high in acid production capacity, the pH can be rapidly reduced, the fermentation time is shortened, and the production efficiency is improved.
Absstract of: CN121951013A
The invention discloses a method for detecting food-borne pathogenic bacteria based on Argonaute protein in combination with loop-mediated isothermal amplification and electrochemical biosensing, and belongs to the technical field of microbiological detection and biosensing. The integrated detection method based on Argonaute protein, loop-mediated isothermal amplification and electrochemical biosensing, which is high in sensitivity, strong in specificity, simple and convenient to operate and suitable for on-site rapid detection, is provided aiming at the problems that an existing food-borne pathogenic bacterium detection method is insufficient in sensitivity, depends on complex instruments, is difficult to apply on site and the like. Target nucleic acid is subjected to exponential amplification through LAMP (loop-mediated isothermal amplification), then Argonaute-mediated two-stage specific recognition and signal transduction are performed, and finally reading is performed through a high-sensitivity electrochemical platform, so that the detection limit of salmonella typhimurium as high as 1 CFU/mL is realized, and the detection range can cover 100-108 CFU/mL.
Absstract of: CN121950636A
The invention discloses bacillus velezensis JL40 with antibacterial and preservative effects, and belongs to the field of microorganisms. The strain has good biological safety, and antibacterial substances produced by metabolism of the strain can keep stable antibacterial activity under extreme pH, high temperature and various organic solvent environments. Experiments show that the JL40 has a remarkable inhibition effect on food-borne pathogenic bacteria such as escherichia coli, staphylococcus aureus, salmonella and listeria monocytogenes, and meanwhile, the JL40 shows a relatively strong inhibition capability on main pathogenic bacteria such as edwardsiella tarda, aeromonas hydrophila and citrobacter buchneri in aquaculture. In the aspect of agricultural application, infection of banana fruit putrefying bacteria can be reduced, and banana decay is reduced. Therefore, the bacillus velezensis JL40 and the metabolites thereof have wide application potential in the aspects of replacing traditional chemical preservatives, inhibiting food-borne pathogenic bacteria and putrefying bacteria, prolonging the preservation time of the picked bananas and the like.
Absstract of: CN121942904A
The invention discloses a plant lactobacillus CCFM1296 strain for improving free amino acid content and in-vitro bacteriostatic ability of fermented milk, and belongs to the technical field of microbial lactobacillus. According to the fermented milk prepared by the plant lactobacillus CCFM1296, the content of free amino acid can be increased, the content of flavor amino acid can be increased, the loss of sweet amino acid can be reduced, and the inhibition effect on the biofilm forming ability of salmonella typhimurium, pseudomonas aeruginosa and pseudomonas fluorescens can be enhanced.
Absstract of: WO2026087718A1
The present invention, in embodiments, relates to a multivalent vaccine comprising a combination of two or more different monovalent Shigella oligosaccharide protein conjugates and an adjuvant. One of the vaccine components can be a Shigella sonnei oligosaccharide- protein conjugate and the other three can be Shigella flexneri oligosaccharide-protein conjugates, specifically, S. flexneri 2a, S. flexneri 3a and S. flexneri 6, respectively. All conjugates feature sOS components acting as surrogates of the heterogeneous Shigella surface polysaccharide antigens characteristic of each one of the selected Shigella serotypes. The multivalent vaccine of the present invention is made for the prevention and/or treatment of disease caused by Shigella, in particular S. sonnei and S. flexneri.
Absstract of: US20260117162A1
0000 The present disclosure relates to a food poisoning bacteria detection sensor. According to an embodiment, it is possible to provide the food poisoning bacteria detection sensor including: a substrate part; a liquid crystal part that is formed on the substrate part and is composed of a micro partition wall partitioning a unit pixel and a liquid crystal structure layer disposed inside the partitioned unit pixel; and an accommodating part that accommodates the substrate part and the liquid crystal part and includes an aqueous solution.
Absstract of: WO2026087662A1
The present invention relates to a combination therapy against pathogenic bacteria in the gut, wherein a bacteriophage against the capsule of the pathogenic bacterium is combined with a vaccination against a bacterial antigen which is normally masked by the capsule.
Absstract of: WO2026089256A1
The present invention relates to an antibacterial composition against pathogenic bacteria, the composition comprising streptococcus salivarius g7 and lactobacillus plantarum gspe05, and specifically, exhibits high antibacterial activity against one or more bacteria selected from the group consisting of Bacillus cereus, Listeria monocytogenes, Salmonella typhimurium, Streptococcus mutans, Porphyromonas gingivalis, and Moraxella catarrhalis, and thus can contribute to the improvement of intestinal diseases, oral diseases, and respiratory diseases caused by these pathogenic strains.
Absstract of: CN121931266A
The invention provides a primer probe combination, a kit, a nucleic acid detection method and application, and relates to the technical field of biochemical detection. The primer probe combination comprises a first primer pair and a first probe, wherein the first primer pair and the first probe are used for specifically amplifying pseudomonas aeruginosa; the second primer pair and the second probe are used for specifically amplifying enterococcus; the third primer pair and the third probe are used for specifically amplifying staphylococcus aureus; the fourth primer pair and the fourth probe are used for specifically amplifying the salmonella enterica. According to the primer and probe combination, a specific nucleotide sequence is utilized, efficient and accurate recognition of pseudomonas aeruginosa, enterococcus, staphylococcus aureus and salmonella enterococcus is achieved in a complex intestinal environment, and non-specific interference is effectively avoided; meanwhile, synchronous quantitative detection of four pathogens is supported, the detection efficiency is remarkably improved, and an accurate and sensitive detection means is provided for rapid clinical evaluation of flora conditions.
Absstract of: CN121930141A
The invention belongs to the field of medicinal chemistry, and discloses an aromatic pleuromutilin derivative with an antibacterial effect as well as a preparation method and application of the aromatic pleuromutilin derivative. The pleuromutilin is structurally modified, aromatic group modification is further increased through a reductive amination method after mercaptoethylamine is introduced into a C-14 side chain, and the structural formula of the pleuromutilin is shown in the specification. Research on an antibacterial mechanism finds that the series of derivatives can effectively interfere with expression of bacterial protein and show unique and excellent antibacterial activity, and the pleuromutilin can be applied to preparation of antibacterial agents. Compared with pleuromutilin in the prior art, most of the pleuromutilin compounds have antibacterial activity on staphylococcus aureus, escherichia coli, salmonella, micrococcus luteus and enterococcus faecalis which are common in in-vitro clinical application, hardly generate drug resistance, have stable safety and are expected to be further optimized into lead compounds of novel antibiotics.
Absstract of: CN121930997A
The invention discloses application of pseudomonas in treatment and/or prevention of Alzheimer's disease, and belongs to the technical field of microorganisms. The invention provides pseudomonas salmonella E14-1 for treating and/or preventing Alzheimer's disease, and the pseudomonas salmonella E14-1 can improve the autophagy level of nematodes and the unfolded protein reaction of endoplasmic reticulum, can reduce the latency period of AD mouse water maze, and can increase the number of platform crossing times. The invention further provides a composition for treating and/or preventing the Alzheimer's disease, a microbial agent and application. The composition and the microbial agent can effectively relieve the symptoms of the Alzheimer's disease.
Absstract of: CN121931058A
The invention discloses a salmonella virulent bacteriophage vBSalPNW15 and application thereof, the bacteriophage is separated from farm sewage, belongs to a long-tail bacteriophage family, has a regular icosahedron head and a long-tail structure, and keeps stable under the conditions that the pH is 4-13 and the temperature is 40-60 DEG C. Genomic analysis shows that the kit does not contain virulence genes and drug-resistant genes and is good in safety. The bacteriophage and cinnamyl aldehyde are combined to be used for preventing and treating salmonella infection, both the bacteriophage and cinnamyl aldehyde show a remarkable synergistic bacteriostatic effect (FICI = 0.5) in vitro and in vivo, and the bacteriophage and cinnamyl aldehyde can effectively inhibit growth of multi-drug-resistant salmonella, remove biological membranes, relieve tissue pathological damage and remarkably improve the survival rate of chicks. The invention provides a safe and efficient antibiotic replacement scheme for preventing and treating salmonella infection, and is suitable for the fields of livestock and poultry breeding and food safety.
Absstract of: CN121930983A
The invention relates to the technical field of aquaculture microorganisms, in particular to enterococcus faecalis, low-temperature probiotic compound bacteria as well as a preparation method and application of the enterococcus faecalis and the low-temperature probiotic compound bacteria. The low-temperature probiotics used in the invention can keep good growth ability and functional activity in a low-temperature environment. The low-temperature probiotic compound bacteria are added into the feed for feeding, so that the intestinal health of cultured objects in a low-temperature culture environment can be effectively improved, and the abundance of pathogenic bacteria such as vibrio, salmonella and edwardsiella can be effectively reduced. The probiotic compound bacteria can effectively increase the protein content in the cultured objects, and compared with cultured objects without probiotics, the protein content in fish meat of the cultured objects can be increased by 8.4% when the cultured objects are continuously fed with the probiotic compound bacteria for 40 days.
Absstract of: CN121915177A
The invention belongs to the technical field of microbiological detection, and particularly relates to a primer and a method for detecting salmonella and a drug-resistant gene thereof and application of the primer and the method. The gene of the salmonella is invA, and the drug resistance genes are blaCTX-M, blaNDM-1, blaTEM-1 and blaOXA; the gene of the salmonella is invA, and the drug resistance genes of the salmonella are blaCTX-M, blaNDM-1, blaTEM-1 and blaOXA; the primers comprise inner primers and outer primers of invA, blaCTX-M, blaNDM-1, blaTEM-1 and blaOXA, a primer group is designed according to conserved regions of a salmonella invA gene and part of beta-lactam drug-resistant genes blaNDM-1, blaTEM-1, blaOXA and blaCTX-M, a detection method of SYBR-LAMP and HNB-LAMP technologies is established, and screening of part of beta-lactam drug-resistant genes carried by animal-derived pathogenic strains is realized.
Absstract of: CN121914893A
The invention relates to a preparation method of a biological agent synergistically produced by bacillus and lactobacillus plantarum, the biological agent and application, and relates to the technical field of bacteriocin preparation. The liquid culture medium contains yeast extract powder and glucose obtained by performing differential temperature asynchronous enzymolysis on starch slurry; s2, uniformly spraying the submerged fermentation liquid obtained in the step S1 on the surface layer of a solid fermentation culture medium within the thickness of 0.5-1 mm for primary fermentation, inoculating lactobacillus plantarum FJS003 into the surface layer of the solid fermentation culture medium within the thickness of 2-3 mm for secondary fermentation after the primary fermentation is finished, and obtaining the production biological agent after the secondary fermentation is finished. The prepared biological agent can effectively inhibit the growth speed of escherichia coli, salmonella and staphylococcus aureus, and meanwhile can effectively degrade aflatoxin B1 and the content of lead, arsenic, mercury and cadmium.
Absstract of: CN121914230A
The invention discloses bacteriocin AM12LCIr as well as a preparation method and application of the bacteriocin AM12LCIr. The invention provides a bacteriocin AM12LCIr, the coding sequence of the bacteriocin AM12LCIr is composed of 46 amino acids, the relative molecular weight is 5067.73 Da, homology analysis shows that the bacteriocin AM12LCIr has the highest homology with class II bacteriocin LCI bacteriocin, but the AM12LCIr and LCI have obvious differences in structure and physicochemical properties, and the bacteriocin AM12LCIr belongs to a novel bacteriocin. According to the present invention, the bacteriocin AM12LCIr is subjected to heterologous expression, and the prepared recombinant protein has good inhibition effect on a variety of bacteria, can inhibit Escherichia coli, Listeria monocytogenes, Enterobacter aerogenes, Salmonella paratyphi B, Staphylococcus aureus and Bacillus cereus, has broad-spectrum antibacterial activity, can be used for preparing more antibacterial products, and has broad application prospects. The invention has important significance for clinically preventing and treating a plurality of pathogenic bacteria including escherichia coli.
Absstract of: CN121914217A
The invention belongs to the field of biological medicine, and relates to broad-spectrum antibacterial peptide derived from periplaneta americana and anti-tumor application of the broad-spectrum antibacterial peptide. The PA-1 and the PA-2 are both novel alpha-spiral cationic peptides and show remarkable inhibitory activity on pseudomonas aeruginosa, salmonella typhimurium, staphylococcus aureus, staphylococcus epidermidis and carbapenem-resistant acinetobacter baumannii, and the minimum inhibitory concentration on the carbapenem-resistant acinetobacter baumannii reaches 11 mu mol/L. In addition, PA-1 and PA-2 can also obviously inhibit the growth of human colon adenocarcinoma cells SW480, cervical cancer cells Hela and gastric cancer cells HGC-27, and show better anti-tumor potential. And the PA-1 and the PA-2 have good thermal stability, pH stability, salt stability and enzyme stability. The two novel active peptides PA-1 and PA-2 provided by the invention show relatively high medicinal values in the antibacterial and anti-tumor fields, and particularly provide a new choice for clinical treatment of carbapenem-resistant acinetobacter baumannii infection.
Absstract of: CN121914985A
The invention discloses a cocktail preparation based on duck-origin salmonella adaptive evolution bacteriophage and application of the cocktail preparation, and belongs to the technical field of biological medicine. The cocktail preparation consists of a phage phi ST 705 with the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 2025836, a phage phi R2 with the preservation number of CCTCC NO: M 2025837 and a phage phi R1-1-1 with the preservation number of CCTCC NO: M 2025838. The bacteriophage phi ST 705 is separated and purified from sewage of a farm, and the host bacterium is salmonella ST 705; the bacteriophage phi R2 and the bacteriophage phi R1-1-1 correspond to bacteriophages separated from the salmonella ST 705 in an adaptive evolution strain under the continuous selection pressure of the bacteriophage phi ST 705. The cracking capacity and the host spectrum of the cocktail preparation are remarkably expanded, and the resistant mutation rate of salmonella ST 705 can be effectively reduced.
Absstract of: WO2026085295A1
Provided herein are kits, compositions, systems, and methods for treating a subject with a tumor with kynurenine responsive and degrading bacteria (e.g., such that the bacteria reduces or eliminates the tumor). In certain embodiments, the kynurenine responsive bacteria comprise one or more nucleic acid sequences encoding: i) a kynurenine transcriptional regulator protein, ii) a kynurenine transporter protein, iii) a kynureninase (KynU) protein, and iv) at least one protein important or essential for growth of the bacteria expression of which is linked to a kynurenine responsive promoter (e.g., where the corresponding gene(s) has been deactivated or knocked out in the genome of the bacteria).
Absstract of: WO2026084839A1
The present disclosure provides a Salmonella therapeutic delivery system having a modified surface for evading the host immune system, and methods of use thereof.
Nº publicación: CN121892129A 21/04/2026
Applicant:
NINGBO CIXI INST OF BIOMEDICAL ENGINEERING
NINGBO INST OF MATERIALS TECHNOLOGY AND ENGINEERING CHINESE ACADEMY OF SCIENCES
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\u5B81\u6CE2\u6148\u6EAA\u751F\u7269\u533B\u5B66\u5DE5\u7A0B\u7814\u7A76\u6240
Absstract of: CN121892129A
The invention discloses a bimetallic nano-enzyme as well as a preparation method and application thereof, and relates to the technical field of biological detection. The bimetallic nano-enzyme is a DNA-Ag/Pt bimetallic nano-enzyme material prepared on the basis of a specific DNA template sequence or containing the specific DNA template sequence, and can be combined with a double-emission ratio type silver nano-cluster to be used for double-signal detection of vibrio parahaemolyticus. The bimetallic nano-enzyme has peroxidase-like activity, the enzyme activity efficiency is adjustable, and the bimetallic nano-enzyme can be used for double-signal detection of vibrio parahaemolyticus. Compared with the prior art, the color development absorbance of the bimetallic nano-enzyme provided by the invention is improved by about 15%, the lowest detection limit of a colorimetric detection method is 10 CFU/mL, and the lowest detection limit of a fluorescence detection method is 1 CFU/mL; the method has no cross reaction on staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, salmonella, vibrio vulnificus and the like, and is suitable for on-site rapid screening, visual interpretation and semi-quantitative detection of scenes such as water samples and marine products.
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