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95
results.
Updated on
05/07/2025 [07:45:00]
Results 50 to 75 of 95
Absstract of: CN119901919A
The invention discloses a capture probe, a detection probe, a detection system and a detection method for escherichia coli O157: H7. The capture probe comprises a magnetic bead capture probe containing rich amino groups and a specific detection probe (nano V-MnO2 (at) ZIF-90/Apt) with an escherichia coli O157: H7 aptamer, a detection system combining a capture probe and a detection probe is adopted to capture and recognize escherichia coli O157: H7 in a sample, a Fe3O4 (at) SiO2-NH2-bacteria-V-MnO2 (at) ZIF-90/Apt compound is formed, reaction is started under specific conditions, a colorimetric signal value is determined according to absorbance at 417 nm, or a colorimetric card is adopted for colorimetry, or the temperature difference before and after 808 nm laser irradiation is adopted, quantitative analysis of escherichia coli O157: H7 is performed, and the detection result is accurate. The detection requirements of rapidity and field applicability are met, and the method has application value in the field of food safety detection.
Absstract of: AU2023312737A1
Provided herein are vaccine composition comprising a
Absstract of: CN119899887A
The invention discloses a high-throughput reverse screening method for functional probiotics for preventing salmonella typhimurium, belongs to the technical field of microorganisms, and is a high-throughput reverse screening method based on a plate agglutination test, and the functional probiotics capable of secreting salmonella typhimurium immune analogues can be rapidly and efficiently screened out. The screening efficiency is greatly improved, the screening cost is greatly reduced, and the screened functional probiotics can prevent salmonella typhimurium infection, are safe and non-toxic, have no side reaction, have long immune duration and have important clinical application value; the functional probiotics can be prepared into functional probiotic vaccines or oral products, infection of salmonella typhimurium in clinical breeding is effectively prevented, the death rate of ducks is remarkably reduced, and economic benefits are improved.
Absstract of: CN119876374A
The invention relates to the technical field of molecular biology, in particular to a biomarker for detecting salmonella enteritidis infection and application of the biomarker in preparation of a salmonella enteritidis infection detection kit. The invention provides a biomarker for detecting salmonella enteritidis infection, and the biomarker is long-chain RNA (Ribonucleic Acid) Lnc012227. The invention provides a novel molecular marker which can be used for diagnosing and monitoring the infection condition of salmonella in duck ovaries, the characteristic can be used as a reference for evaluating the resistance potential of offspring, a novel strategy and a tool are provided for prevention and control of salmonella infection in the laying duck industry, and the application prospect is wide. The reproductive performance of the laying ducks and the safety of the duck eggs are favorably improved.
Absstract of: CN119875984A
The invention discloses a salmonella YeaZ gene deleted strain as well as a construction method and application thereof, and belongs to the technical field of gene engineering. The YeaZ gene deletion strain is constructed by utilizing a lambda-Red homologous recombination technology, and compared with a WT strain, the constructed salmonella YeaZ gene deletion strain can enter a VBNC state more easily and cannot revive in the VBNC state, and pathogenic bacteria in food can be detected by utilizing the characteristics of special biological phenotype and difficulty in reviving of the salmonella YeaZ gene deletion strain.
Absstract of: CN119876424A
The invention discloses a chicken salmonella enteritidis infection related biomarker and application thereof, and belongs to the technical field of poultry genetic breeding and propagation. The invention finds that the content of RUNX2 transcription factors in cecum T cells infected by salmonella enteritidis is obviously different, the expression quantity of RUNX2 in a susceptible group is far higher than that of RUNX2 in a resistance group, which indicates that the RUNX2 is a susceptible gene infected by salmonella enteritidis, and if a chicken shows typical enteritis symptoms such as diarrhea, inappetence and the like and the expression level of RUNX2 is detected to be obviously increased, the RUNX2 is a susceptible gene infected by salmonella enteritidis. This may be a marker of a chicken susceptible to Salmonella enteritidis; if the chicken does not show corresponding symptoms and the expression level of the RUNX2 is not greatly increased, the chicken is indicated to have resistance to the salmonella enteritidis, the resistance of the chicken to the infected salmonella enteritidis can be identified on the molecular level through a single-cell transcriptomics technology, and a technical support is provided for breeding poultry varieties resistant to salmonella enteritidis infection.
Absstract of: CN119875946A
The invention belongs to the technical field of agricultural biology, and particularly relates to a strain of lactobacillus coryniformis HAU121, a microbial inoculum and application of the microbial inoculum. The microbial inoculum comprises lactobacillus coryniformis HAU121, lactobacillus coryniformis HAU121, lactobacillus coryniformis HAU121, lactobacillus coryniformis HAU121 and lactobacillus coryniformis HAU121. According to the invention, a strain of lactobacillus clavuliformis HAU121 is screened from rumen fluid of dairy cow, the strain has stable acid production performance, and the stable acid production performance ensures that the strain rapidly inhibits the growth of other microorganisms in the fermentation process; bacteriostatic activity detection finds that the strain HAU121 has different degrees of inhibition capability on escherichia coli, staphylococcus aureus, salmonella and pseudomonas aeruginosa. The strain HAU121 provided by the invention is applied to solid anaerobic fermentation of cottonseed meal, the removal rate of free gossypol in the fermented cottonseed meal can reach 75.20%, and the strain HAU121 has no obvious negative influence on the nutritional value of the cottonseed meal, and has important application value in the aspect of feed development and utilization of the cottonseed meal.
Absstract of: CN119875903A
The invention provides a preparation method of an antibiotic carrier based on bacterial outer membrane vesicles, which is characterized by comprising the following steps: (1) culturing bacteria in a culture medium containing antibiotics; and (2) removing viable bacteria in the culture product, and extracting the outer membrane vesicles of the bacteria to obtain the antibiotic carrier based on the outer membrane vesicles of the bacteria, wherein the bacterium is a salmonella mutant strain QS0074, and the preservation number of the bacterium is CCTCC (China Center For Type Culture Collection) M 2023192; the antibiotic is alcamicin. The antibiotic carrier based on the bacterial outer membrane vesicles, provided by the invention, has excellent drug loading capacity on the acamicin, and has relatively good drug activity stability; the preparation method is simple and easy to popularize.
Absstract of: CN119875961A
The invention relates to a VBNC salmonella enteritidis resuscitation culture medium and application, and belongs to the technical field of microorganism culture detection. The VBNC salmonella enteritidis resuscitation culture medium comprises 8 g/L-12 g/L of peptone, 3 g/L-7 g/L of sodium chloride, 7 g/L-11 g/L of disodium hydrogen phosphate, 1 g/L-2 g/L of monopotassium phosphate and 0 U/mL-1000 U/mL of Rpf recombinant protein. Experiments prove that the culture medium can detect VBNC salmonella enteritidis which cannot be detected by a traditional culture medium, and the VBNC salmonella enteritidis resuscitation culture medium is proved to be excellent in performance and high in detection rate.
Absstract of: CN119876412A
The invention relates to a CNTN5 gene related to the resistance character of host salmonella pullorum and an SNP molecular marker and application thereof, the CNTN5 gene and the SNP molecular marker thereof are used for breeding or auxiliary breeding of chicken varieties with high resistance to salmonella pullorum, the nucleotide sequence of the CNTN5 gene is as shown in SEQ ID NO.1, the SNP molecular marker of the CNTN5 gene is located in the intron region of the CNTN5 gene of the first chromosome of the chicken, and the SNP molecular marker of the CNTN5 gene is located in the intron region of the CNTN5 gene of the first chromosome of the chicken. At the site 185063016bp of the chromosome 1, the polymorphism is T or C; when the basic group at the SNP molecular marker of the CNTN5 gene is T, the chicken has high resistance to salmonella pullorum; when the basic group at the SNP molecular marker of the CNTN5 gene is C, the chicken has low resistance to salmonella pullorum. By utilizing the CNTN5 gene and the SNP molecular marker provided by the invention, a chicken variety with high resistance to salmonella pullorum can be quickly and effectively screened and bred.
Absstract of: CN119881118A
The invention belongs to the technical field of food-borne pathogenic bacterium detection, and discloses a detection method for targeted analysis and detection of salmonella based on a characteristic peptide, and the method detects salmonella pollution in a dairy product by analyzing the following characteristic peptides: I1: (LLADDLVPSR; i2: (IGTISTTGEMSPLDAR), and I3: ( I3: (IELALFLDSYIPEPER), and a method for preparing the same. And I4: (AVAAVEELK). The method for identifying the type of the strain by taking the characteristic peptide as an index is a relatively safe food detection method, the characteristic peptide of a specific microorganism is used for screening, and the characteristic peptide is detected through a targeted mass spectrometry analysis technology, so that the detection of pathogenic bacteria is converted into the detection of the characteristic peptide. The method has the advantages of rapidness, high throughput, high reliability, no dependence on bacterial colony purity and the like. By obtaining a characteristic peptide combination with good specificity, the technology can realize detection of various specific microorganisms.
Absstract of: CN119876046A
The invention discloses a salmonella bacteriophage JN06 and an application thereof. The preservation number of the salmonella phage JN06 is GDMCC (China General Microbiological Culture Collection Center) No: 65479-B1, and the salmonella phage JN06 is preserved in Guangdong Province Microbial Culture Collection Center on November 13, 2024. The Salmonella lyase has the advantages that the Salmonella lyase is simple in preparation method and wide in host spectrum, not only can be used for lysing various serotypes of salmonella, particularly can be used for lysing Salmonella Schwarzenge Salmonella vaporariorum, but also can be used for lysing Escherichia coli at the same time. The method has the advantages of short incubation period, high outbreak amount, strong tolerance to temperature and pH value, wider application range, and no virulence gene and drug-resistant gene.
Absstract of: CN119876330A
The invention discloses a method for achieving the antibacterial purpose through cooperation of natural plant essential oil and potassium hydrogen persulfate composite salt, and relates to the technical field of antibacterial treatment. According to the method for synergistically achieving the antibacterial purpose through the natural plant essential oil and the potassium hydrogen persulfate composite salt, the natural plant essential oil with high antibacterial activity is obtained by testing the antibacterial effect of different natural plant essential oil when the natural plant essential oil is independently used, and the natural plant essential oil and the potassium hydrogen persulfate composite salt are compounded through a chessboard method, so that the antibacterial effect of the natural plant essential oil is achieved. The bacteriostatic effect of the compound solution is quantitatively analyzed through part of bacteriostatic concentration indexes, accurate reference data is provided for judgment of the interaction relation between the natural plant essential oil and the potassium hydrogen persulfate compound salt, support is provided for improvement of bacteriostatic efficiency, reduction of the drug use amount and expansion of a bacteriostatic spectrum, and the method has a wide application prospect. The defects of single natural plant essential oil in use are overcome, the cost and the use dosage are reduced, and the natural plant essential oil has a good applicati
Absstract of: CN119876431A
The invention discloses a primer group for detecting polymyxin drug resistance genes (mcr-1 to mcr-10) of bacteria, a multiplex PCR (Polymerase Chain Reaction) method and application. The primer group comprises 10 pairs of primers which are respectively used for carrying out specific PCR (Polymerase Chain Reaction) amplification on mcr-1 to mcr-10 genes. Based on the primer group, a one-step multiplex PCR method for efficiently detecting the genes from mcr-1 to mcr-10 is established. According to the method disclosed by the invention, efficient, specific and sensitive detection of mcr-1 to mcr-10 genes can be realized through one-step reaction of a reaction system. The method provided by the invention is used for detecting escherichia coli and salmonella isolates and comparing with a genome sequencing result, so that the accuracy of the method provided by the invention is further verified. The invention fills the blank of lack of mcr-1 to mcr-10 gene multiplex PCR primer groups and methods at present, and has good application prospects in the fields of clinical detection and microbial prevention and control.
Absstract of: CN119868246A
The invention belongs to the field of oral care products and provides antibacterial mouthwash which is prepared from the following raw materials in percentage by mass: 20-30% of lactobacillus plantarum powder, 25-30% of plant extract, 10-15% of humectant, 2-3% of preservative and 0.5-2% of pH regulator. 5-12% of an antibacterial synergist; 2-3% of a sweetening agent and 17-35% of deionized water; the plant extracts comprise a honeysuckle flower extract, a Philippine violet herb extract, a left-hand fragrant extract, a tea leaf extract and a licorice root extract; lactobacillus plantarum powder is provided by Shanghai Shanghai Shanghai Shanghai Detection Technology Co., Ltd., and the product number is BNCC299253. The toothpaste can play a good role in protecting helicobacter pylori, salmonella, listeria monocytogenes, enterobacter sakakaakii and the like, can relieve swelling and aching of gum, oral mucosa bleeding and other symptoms, and avoids halitosis. And the toothpaste also has a very good prevention effect on diseases such as oral ulcer, gingivitis and periodontitis.
Absstract of: US2025127889A1
The present disclosure relates to a composition for inducing migration of activated B cells to a germinal center, the composition including a substance that induces CD11b expression in B cells, a production method therefor, or use thereof. In one aspect, the present disclosure relates to immunostimulation and maintenance or improvement of mucosal flora using the substance that induces the CD11b expression in B cells.
Absstract of: AU2023262222A1
The invention relates to a gelled reaction medium for the detection, identification, enumeration and/or isolation of at least one target microorganism in a sample that may contain same, comprising at least one binding partner specific to a component of a target microorganism or of a component derived from said microorganism, coupled to at least one nanoparticle to form at least one binding conjugate.
Absstract of: WO2025081515A1
The present invention provides a tumor neoantigen vaccine system specifically marking a heterologous protein, and a use thereof. The vaccine system is composed of a heterologous protein mRNA vaccine and a recombinant oncolytic virus; the heterologous protein mRNA vaccine is used to induce an organism to generate heterologous protein-specific memory T cells, and the recombinant oncolytic virus is used to promote a tumor to express microorganism-derived tumor neoantigens. When pre-immunized memory T cells detect these marked specific neoantigens, the tumor expressing the neoantigens is quickly activated and killed, the release of tumor autoantigens is promoted, dendritic cells phagocytize antigens to further initiate an anti-tumor response, and a systemic anti-tumor immune effect is activated by means of antigenic epitope spreading.
Absstract of: WO2025081938A1
Provided is a method for preparing a biofloc culture medium by using organic wastes. The method comprises: adding a first auxiliary material to organic wastes, uniformly mixing same to obtain a mixture, and adjusting the water content of the mixture to 45-55%; inoculating an activated Bacillus subtilis seed culture, Bacillus licheniformis seed culture and Saccharomyces cerevisiae seed culture into the mixture according to a ratio, and performing fermentation with stirring and ventilation, so as to obtain a fermented product; adding a second auxiliary material to the fermented product, and uniformly mixing same to obtain the biofloc culture medium. In the finished biofloc product prepared by the method, the viable count of Bacillus subtilis is greater than or equal to 400 million cfu/g, the viable count of Bacillus licheniformis is greater than or equal to 200 million cfu/g, the viable count of Saccharomyces cerevisiae is greater than or equal to 1200 million cfu/g, the total number of bacterial colonies is greater than or equal to 8000 million cfu/g, and no salmonella is detected. The present application can solve the pollution problem of livestock and poultry manure and further achieves recycling utilization of livestock and poultry manure.
Absstract of: CN119846199A
The invention relates to the technical field of analysis and detection, in particular to an immunochromatography test strip for detecting shiga toxin-producing escherichia coli O103. Based on the characteristics of the quantum dots and the fluorescent microspheres, a probe is synthesized by utilizing the purified protein expressed by the pET-28a-O103 strain and the fluorescent microspheres, and the immunochromatography rapid detection test strip for identifying the shiga toxin-producing escherichia coli O103, which is simple to operate, is established, can be used for rapidly identifying the shiga toxin-producing escherichia coli O103 clinically, and can be used for rapidly detecting the shiga toxin-producing escherichia coli O103. The method has important significance on prevention and control of Escherichia coli diseases.
Absstract of: CN119842745A
本发明公开了鼠伤寒沙门菌毒力基因Ict的鉴定及其减毒疫苗候选株的制备与应用。本发明选择强毒力鼠伤寒沙门菌SL1344株作为亲本株，将其中降解代谢衣康酸的关键毒力基因Ict进行敲除，构建鼠伤寒沙门菌SL1344减毒疫苗候选株。通过小鼠动物毒力实验、免疫保护力实验和安全性实验等证实该疫苗候选株SL1344‑ΔIct缺失株，不仅对动物的致病力显著降低，且提供了良好的免疫原性和免疫保护力。本发明实现对强毒力鼠伤寒沙门氏菌株的减毒，可广泛应用于沙门菌的防控，为研制沙门菌的减毒活疫苗及活载体疫苗奠定基础。
Absstract of: CN119842544A
The invention belongs to the field of microorganisms, and relates to lactobacillus rhamnosus and application thereof. The lactobacillus rhamnosus KC3 is separated from Xinjiang healthy bactrian camel milk, and the lactobacillus rhamnosus KC3 has the activity of inhibiting various pathogenic bacteria such as escherichia coli, klebsiella pneumoniae, salmonella, proteus mirabilis, staphylococcus aureus, staphylococcus epidermidis, listeria monocytogenes and streptococcus agalactiae. The metabiotics of the strain can enhance the sensitivity of pathogenic bacteria such as escherichia coli and staphylococcus aureus to antibiotics such as beta-lactam antibiotics, and can synergistically enhance the antibacterial effect of the beta-lactam antibiotics to the pathogenic bacteria such as escherichia coli, staphylococcus aureus, CRE or MRSA. The lactobacillus rhamnosus KC3 metagen can also destroy cell walls of pathogenic bacteria such as escherichia coli, staphylococcus epidermidis, CRE or MRSA and the like, and the formation of bacterial biofilms is inhibited.
Absstract of: CN119855897A
The present invention provides a method for producing a recombinant bacterium, which is characterized in that the recombinant bacterium is used for producing a recombinant bacterium, and the recombinant bacterium is obtained by using a recombinant bacterium, which is preserved in the Deersche Sammlarg von Mikroorgani sung Zel lkulturen and has been preserved in the Deersche Sammlarg von Mikroorgani sung Zel lkulturen with a preservation number of DSM 32786. The present invention relates to a method for resisting Salmonella typhimurium infection by using a culture of Lactobacillus rhamnosus LRH10 having a preservation number of DSMZ GmbH, Lactobacillus paracasei LPC12 having a preservation number of DSMZ GmbH, Lactobacillus fermentum LF26 having a preservation number of DSMZ GmbH, Streptococcus thermophilus ST30 having a preservation number of DSMZ 32788, and Lactobacillus helveticus LH43 having a preservation number of DSMZ 32787, and more specifically, to a method for resisting Salmonella typhimurium infection by using a culture of Lactobacillus rhamnosus LRH10 having a preservation number of DSMZ GmbH.
Absstract of: WO2025080776A2
Aspects of the invention described herein relate to a vaccine composition comprising at an antigenic polysaccharide and an immunomodulatory amount of a sialic acid binding moiety, wherein the sialic acid binding moiety comprises sialic acid binding domain (SBD) which is not fused to an antigenic polypeptide.
Nº publicación: GB2634693A 16/04/2025
Applicant:
SHANGDONG PROVINCIAL CENTER FOR ANIMAL DISEASE CONTROL SHANDONG PROVINCIAL CENTER FOR ZOONOSES EPIDE [CN]
BEIJING SENKANG BIOTECHNOLOGY DEV CO LTD [CN]
Shangdong Provincial Center for Animal Disease Control (Shandong Provincial Center for Zoonoses Epidemiology Investigation and Surveillance),
Beijing Senkang Biotechnology Development Co., Ltd
Absstract of: GB2634693A
A duplex fluorescent PCR detection primer probe group of duck Salmonella enteritidis and Salmonella typhimurium, a kit, and the use thereof. Comparative analysis on the whole genomes of Salmonella enteritidis and Salmonella typhimurium is performed to screen out as identification targets a specific fragment Sdf I of lyg D gene of Salmonella enteritidis and a specific gene STM4497 of Salmonella typhimurium, and the present application designs specific primers and probes.
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