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Traditional Chinese medicine composition for inhibiting salmonella choleraesuis as well as preparation and application thereof

Resumen de: CN121466192A

The invention discloses a traditional Chinese medicine composition for inhibiting salmonella choleraesuis as well as a preparation and application thereof, and belongs to the technical field of traditional Chinese medicine and network pharmacology. According to the invention, traditional Chinese medicines with a good antibacterial effect on S. Choletaesuis are screened, and the compound five-plum decoction with a good antibacterial effect on S. Choletaesuis is established. The five-plum decoction can be used for effectively regulating and controlling the steady state of intestinal flora, and reducing the oxidative damage and inflammatory damage of the S. Cholera to the intestinal tract and the liver by regulating and controlling the signal channels of TLR4/NF-kB/MyD88 and ZO-1/Claudin-1/Occludin. Through the multiple effects of resisting bacteria, resisting inflammation, protecting intestinal barriers and improving production performance, the five-plum decoction shows remarkable prevention and treatment effects on swine paratyphoid, and has huge value of being developed into a novel veterinary traditional Chinese medicine preparation and put into clinical application.

A YEAST BIOMASS COMPOSITION AND USES THEREOF

Resumen de: WO2026027798A1

A yeast biomass composition is formed by culturing a strain of yeast in a substrate such as whey permeate to product a yeast broth, dewatering the broth to produce yeast biomass, and hydrolysing the yeast biomass. The yeast biomass composition finds utility as a feed additive for broiler chickens to reduce the adherence of pathogenic bacteria like Salmonella and E.coli to the gut wall of the chickens, thereby reducing intestinal stress and improving life daily weight gain in the chickens.

ANTI-ETEC ADHESIN PROTEIN ANTIBODIES AND METHODS OF USE

Resumen de: US20260035440A1

The invention provides anti-ETEC adhesin protein antibodies and methods of using the same.

LIVER DISEASE TREATMENTS USING ATTENUATED STRAINS OF SALMONELLA TYPHIMURIUM

Resumen de: WO2026030756A2

The composition and methods of treatment disclosed or contemplated herein include compositions and methods of treatment for liver-related diseases. The overall treatment strategy would involve the identification of a target protein or receptor within the liver that is responsible for ongoing liver injury. The strain of orally administered Salmonella Typhimurium that colonizes the liver would be engineered to secrete a particular protein or nanobody that would stop and/or reverse the progressive hepatic disease

一种多功能纳米材料荧光-比色双模式食源性致病菌检测与杀灭方法及其系统

Resumen de: CN121454057A

本发明公开了一种多功能纳米材料荧光‑比色双模式食源性致病菌检测与杀灭方法及其系统。该方法利用具有纳米酶活性的金属有机框架材料（MIL‑53(Fe)）作为信号开关与发生器，联合荧光指示与光热杀菌单元的碳纳米点（CDs）共同构成了基础平台，利用适配体对纳米酶活性位点的掩蔽与目标菌触发下的竞争性释放为核心机制，激活比色与比率荧光双信号通道，实现自验证的高精度检测；并利用体系中的CDs作为光热元件，在检测后实现原位光热杀菌。通过更换适配体即可切换检测对象的“平台化”设计，展现了良好的通用性和应用前景，为食源性致病菌的检测与消除提供了灵敏、高效的新策略。

Recombinant salmonella choleraesuis nuclease regulation vector as well as construction method and application thereof

Resumen de: CN121450682A

The invention discloses a recombinant salmonella choleraesuis nuclease regulation vector as well as a construction method and application thereof. After oral administration, the recombinant salmonella choleraesuis nuclease regulatory vector rSC0140 enters host immune cells, c-di-AMP is released, a host STING pathway is activated, and a broad-spectrum antiviral effect can be achieved; the recombinant strain rSC0140 is proved to be capable of activating STING pathways in macrophages and mice, causing antiviral states of the macrophages and the mice and resisting challenge of various influenza viruses; the recombinant strain has broad-spectrum antiviral efficacy and is suitable for preventing and treating various virus diseases clinically.

Monoclonal antibody combination for Brucella detection and application thereof

Resumen de: CN121449738A

The invention discloses a monoclonal antibody combination for Brucella detection and application thereof, and belongs to the technical field of immunodetection. The monoclonal antibody combination disclosed by the invention comprises a monoclonal antibody 15F4 and a monoclonal antibody 32H9. On the basis of the monoclonal antibody combination, a double-antibody sandwich ELISA detection method with 15F4 as a capture antibody and enzyme-labeled 32H9 as a detection antibody is established, and key parameters such as coating concentration, closing conditions, incubation and color development time and the like are optimized. The method has high specificity on brucella, and does not cross with common cross-reactive bacteria such as escherichia coli O: 157, salmonella and the like; the sensitivity is high, and the detection limit can reach 1 * 10 < 5 > CFU/mL; the repeatability is good, and the intra-batch and inter-batch variation coefficients are both smaller than 10%. The invention provides a core reagent and a reliable means for early, rapid and accurate diagnosis of brucellosis, and is suitable for on-site screening of clinical samples and animal epidemic diseases.

Method of diagnosing migraine using oral microbiome

Resumen de: KR20260015111A

본 개시는 편두통 진단을 위한 정보 제공 방법에 관한 것으로서, 보다 상세하게는 생물학적 시료에서 제멜라 헤몰리산스, 센티페다 페리오돈티, 카르디오박테리움 발바룸, 카토넬라 모르비 및 셀레노모나스 녹시아로 이루어진 군에서 선택된 적어도 하나의 박테리아의 상대적 풍부도를 측정하는 단계를 포함함으로써 환자의 통증 또는 거부감 없이 정확하고 신속한 편두통 진단을 가능하게 하는 방법에 관한 것이다.

Broad-spectrum antibacterial peptide KVL4 as well as preparation method and application thereof

Resumen de: CN121426898A

The invention provides a broad-spectrum antibacterial peptide KVL4 as well as a preparation method and application thereof, and belongs to the technical field of biology, and the amino acid sequence of the broad-spectrum antibacterial peptide KVL4 is shown as SEQ ID No.1. Design is carried out on the basis of an alpha-helical peptide template (abcdefg) n, n = 4, positions a, d and f are hydrophobic amino acids, and Val, Leu and Trp are adopted in sequence; positive charge amino acid Lys is adopted at positions b, c and e; and negative charge amino acid Glu is adopted at the position g to promote spiral association. The antibacterial peptide KVL4 has an inhibition effect on various bacteria such as escherichia coli, salmonella typhimurium and staphylococcus aureus, and the average antibacterial activity is as low as 5.84 mu M. And the high-concentration KVL4 shows good biocompatibility on both human red blood cells and porcine jejunum epithelial cells IPEC-J2. In conclusion, the antibacterial peptide KVL4 is an antibacterial peptide with relatively high application potential.

用于检测和确认产生志贺毒素的大肠杆菌和/或肠出血性大肠杆菌的方法

Resumen de: WO2025003119A1

The invention relates to a method for detecting and confirming at least one Shiga toxin-producing Escherichia Coli (STEC) which may be present in a sample comprising enterobacteria, comprising the following steps: - performing lysis of the sample, enabling lysis of the STECs in order to obtain a solution comprising the nucleic acids thereof; - bringing the solution of nucleic acids into contact with primers, making it possible to amplify at least the stx1 and/or stx2 gene or gene fragment; - if at least one of the stx1 and/or stx2 genes or gene fragments is amplified, part of the sample is deposited on an agar reaction medium comprising ■ at least one toxin inducer, ■ at least one agglutinating conjugate formed by at least one binding partner specific to the STX1 protein and/or at least one binding partner specific to the STX2 protein, which binding partner(s) is (are) coupled to a nanoparticle; - detecting and confirming the presence of at least one STEC by the appearance of a halo on the agar around the STEC.

Traditional Chinese medicine waste resource feed additive for promoting growth and enhancing immunity and preparation method thereof

Resumen de: CN121421088A

The invention discloses a traditional Chinese medicine waste resource feed additive capable of promoting growth and enhancing immunity and a preparation method of the traditional Chinese medicine waste resource feed additive. The feed additive is prepared on the basis of medicinal and edible byproducts such as honeysuckle leaf waste, astragalus residues, pericarpium citri reticulatae peel scraps, lycium barbarum seed squeezing residues and ginger peel which are generated in the traditional Chinese medicine processing process. After biotransformation treatments of solid state fermentation, hydrolysis, ultrasonic wall breaking, extraction and the like, the mixture is compounded with yeast cell walls and a plant-based carrier. Feeding test results show that the feed additive can effectively improve lysozyme activity, antioxidant enzyme activity and immune cell phagocytic ability of livestock and poultry, reduce morbidity and death rate caused by common bacterial diseases (such as escherichia coli, salmonella, streptococcus and the like), promote daily gain, improve feed conversion rate and improve overall growth performance. According to the invention, high-valued utilization of waste resources with homology of medicine and food can be realized, a novel functional feed additive is provided for antibiotic-free, green and efficient breeding, and the feed additive is suitable for large-scale animal husbandry production.

Hermetia illucens antibacterial peptide Hill198 and genetic recombination bacillus subtilis thereof

Resumen de: CN121427969A

The invention discloses a hermetia illucens antibacterial peptide Hill198 and genetic recombination bacillus subtilis thereof, and belongs to the technical field of genetic engineering and microorganism application. A bacillus subtilis protease deficient strain WB800N is taken as a starting strain and is transferred into a recombinant expression vector pHT43-Hill198, and recombinant bacillus subtilis for efficiently secreting and expressing the Hill198 protein is obtained by virtue of IPTG (isopropyl-beta-d-thiogalactoside) induction. After the recombinant strain is subjected to induced expression, the fermentation supernatant of the recombinant strain has obvious inhibitory activity on escherichia coli O157: H7 and salmonella typhimurium. The recombinant plasmid still keeps stable inheritance after continuous passage for 30 generations, and insertion of exogenous genes does not affect normal growth characteristics of host bacteria. The recombinant bacterium can be used as a feed additive and is used for preventing and treating intestinal diarrhea diseases of livestock and poultry and aquatic animals caused by gram-negative bacteria such as escherichia coli.

Oligonucleotide composition, kit and application

Resumen de: CN121428177A

The invention relates to the technical field of biological detection, and discloses an oligonucleotide composition, a kit and application. The composition disclosed by the invention can be used for rapidly and simultaneously detecting salmonella, shigella, entamoeba histolytica, vibrio cholerae, hepatitis A virus and hepatitis E virus in a sample with high specificity, and has relatively high sensitivity and stability. Compared with the traditional detection methods such as pathogen separation, genome sequencing and the like, the efficiency of identifying and detecting salmonella, shigella, entamoeba histolytica, vibrio cholerae, hepatitis A virus and hepatitis E virus by adopting the composition disclosed by the invention is effectively improved, the detection process is simple and convenient to operate, and the composition is suitable for large-scale popularization and application.

Lactobacillus johnsonii for preventing and treating salmonella pullorum disease and application of lactobacillus johnsonii

Resumen de: CN121427773A

The invention discloses lactobacillus johnsonii for preventing and treating pullorum disease salmonellosis and application of the lactobacillus johnsonii, and belongs to the field of microorganisms. According to the invention, a strain of Lactobacillus johnsonii LMZ8 is separated and screened, and the preservation number of the strain of Lactobacillus johnsonii LMZ8 is GDMCC No: 66614. The lactobacillus johnsonii LMZ8 is good in acid resistance and has certain tolerance to high-concentration cholate. Experimental results show that the lactobacillus johnsonii LMZ8 can effectively inhibit the growth of various pathogenic bacteria such as salmonella typhimurium and salmonella enteritidis, especially has a prominent inhibition effect on five strains of multi-drug-resistant salmonella pullorum with no same drug resistance spectrum, can effectively prevent and treat the pullorum disease caused by salmonella pullorum, and improves the growth performance of chicks. The lactobacillus johnsonii LMZ8 shows remarkable development potential in the aspects of preventing and controlling food-borne pathogenic bacteria and preventing and treating diseases such as pullorum disease, and has a wide development prospect.

Preparation method and application of Tieguanyin tea residue carbon dots

Resumen de: CN121426096A

The invention discloses a preparation method of Tieguanyin tea residue carbon dots, which comprises the following steps: S1, tea residue pretreatment: soaking Tieguanyin tea leaves in distilled water according to a mass-to-volume ratio of 2.5 g/ml-5 g/ml, filtering, collecting tea residues, and drying the tea residues to obtain tea residue powder; s2, tea residue carbon dot synthesis: uniformly mixing the tea residue powder obtained in the step S1 with water according to a mass volume ratio of 1g: (25-30) ml to obtain a tea residue suspension, transferring the tea residue suspension into a polytetrafluoroethylene reaction kettle, carrying out a hydrothermal reaction, and cooling to room temperature to obtain a reaction solution; s3, primary purification of the tea residue carbon dots: performing suction filtration on the reaction solution obtained in the step S2 to obtain a clear and transparent solution, and performing concentration and centrifugation to obtain supernate. And S4, secondary purification of the tea residue carbon dots: filtering, dialyzing and freeze-drying the supernate obtained in the step S3 to obtain the Tieguanyin tea residue carbon dots. The invention further discloses the tea residue carbon dots prepared by the method and application of the tea residue carbon dots. The raw materials are cheap and easy to obtain, the process is simple, green and environment-friendly, and the prepared Tieguanyin tea residue carbon dots have a good antibacterial effect and

Antibacterial peptide fragment intercepted from collected fly IDGF4 protein and application of antibacterial peptide fragment in preparation of antibacterial drugs

Resumen de: CN121427886A

The invention discloses an antibacterial peptide fragment intercepted from a mined fly IDGF4 protein and application of the antibacterial peptide fragment in preparation of antibacterial drugs. According to the invention, oligopeptide fragments at different parts in the collected fly IDGF4 protein are screened, three candidate peptides are obtained through chemical synthesis, the antibacterial performance of the candidate peptides is systematically evaluated, antibacterial experiment results show that the oligopeptide with the amino acid sequence shown as SEQ ID No.2 can effectively inhibit the growth of salmonella and staphylococcus aureus under the concentration of 1mg/mL, and the oligopeptide with the amino acid sequence shown as SEQ ID No.2 can effectively inhibit the growth of salmonella and staphylococcus aureus under the concentration of 1mg/mL. The antibacterial effect of the peptide is confirmed in inhibition zone experiments, enzyme-labeled turbidimetry and growth curve monitoring, and the peptide shows stable and remarkable antibacterial activity, which indicates that the peptide can be used as a novel antibacterial molecule. The invention provides a new candidate sequence for developing the antibacterial peptide with high efficiency and low drug resistance risk, and has application potential in the aspect of preparing clinical antibacterial drugs.

METHODS OF REDUCING INTRAUTERINE TRANSMISSION OF SALMONELLA DUBLIN INFECTION

Resumen de: WO2026024938A1

Methods of reducing or preventing intrauterine transmission of Salmonella Dublin in a subject, typically a cow, are provided. The methods include a first administration, and optionally second or more administrations, to a pregnant cow of an effective amount of an immunogenic composition to induce an immune response against S. Dublin in the cow. In some forms, the cow is infected with S. Dublin. In some forms, two or more cows are treated in parallel, for example, all cows that inseminated or otherwise became pregnant in parallel. Typically, the first administration, and optionally second or more administrations, are administered to the cow at a time(s) that reduces intrauterine transmission of S. Dublin infection from the cow to one or more of her calves compared to unvaccinated control cows and/or cows administered according to a different administration regimen. Exemplary methods of administration are provided.

ANTI-CANCER STRAIN EXPRESSING STREP-TAG, ANTI-CANCER COMPOSITION USING THE SAME, ANTI-CANCER ADJUVANT AND TUMOR IMAGING AGENT

Resumen de: US20260028377A1

The present invention relates to a gene construct characterized by encoding a fusion protein of flgM and Strep-tag through the linkage of an anti-sigma factor flgM gene and a gene encoding Strep-tag, enabling the expression of Strep-tag on the surface of an anti-cancer strain that can target tumor sites and stimulate immune activity, and to an anti-cancer adjuvant and tumor imaging agent that, when transformed by this construct, exhibits anti-cancer activity itself and can be effectively utilized in the diagnosis and treatment of tumor cells together with anti-cancer substances or contrast agents labeled with substances specifically interacting with Strep-tag.

RECOMBINANT EXPRESSION VECTOR FOR SECRETION OF CHLOROTOXIN, AND ATTENUATED SALMONELLA STRAIN TRANSFORMED BY MEANS OF SAME

Resumen de: US20260027165A1

The present disclosure relates to a recombinant expression vector for secretion of chlorotoxin and an attenuated Salmonella strain transformed therewith, and provides: a recombinant expression vector comprising a flgM gene and a chlorotoxin gene; an attenuated Salmonella strain transformed therewith; and a pharmaceutical composition for cancer treatment, comprising the attenuated Salmonella strain.

Assay for determination of functional immunity against salmonella

Resumen de: GB2700365A

Method of performing SBA assay comprising: preparing media and buffer; preparing bacterial stock with a mother culture stock, media and buffer; diluting bacterial stock with buffer to obtain a diluted bacterial stock; adding test serum, complement, diluted bacterial stock, and buffer to form an assay solution; incubating assay solution, spotting it on an agar plate and incubating the plate; calculating antibody titre to determine the serum bactericidal activity. Kit or system for performing said SBA assay wherein they contain said media, buffer, bacterial stock and complement and optionally a package insert or label providing instructions to perform the SBA assay. Method for performing a serum bactericidal assay comprising preparing a bacterial stock with a mother culture stock and media, diluting the bacterial stock 1:100-1:6000 with buffer to obtain diluted bacterial stock, adding test serum, complement, diluted bacterial stock, and buffer to form an assay solution; incubating the assay solution, spotting it on an agar plate and incubating the plate; calculating antibody titre to determine the serum bactericidal activity. Figure 1

TREATMENT OF NERVOUS SYSTEM TUMORS USING ATTENUATED SALMONELLA TYPHIMURIUM

Resumen de: WO2024197078A2

Provided herein are compositions and methods for treating benign nervous system tumors, including schwannomas, using attenuated mutants of Salmonella typhimurium comprising a spiC deletion, and, optionally, one or more checkpoint inhibitors.

Dual MIRA detection method for type A clostridium botulinum and staphylococcus aureus for portable reagent card incubator and application of dual MIRA detection method

Resumen de: CN121406806A

The invention belongs to the technical field of biological detection, and particularly relates to a dual MIRA detection method for type A clostridium botulinum and staphylococcus aureus for a portable reagent card incubator. The double colloidal gold type MIRA method established by the invention has good specificity on type A clostridium botulinum and staphylococcus aureus, and has no cross reaction with six strains such as pasteurella multocida, klebsiella pneumoniae and salmonella. The lowest detection limits of the method on the staphylococcus aureus and the A-type clostridium botulinum are 2.94 * 100 copies/mu L and 2.27 * 100 copies/mu L respectively. The optimized double colloidal gold type MIRA method can be successfully applied to a reagent card incubator. The detection method disclosed by the invention has the advantages of good specificity, high sensitivity, simplicity in operation, short time consumption and the like.

Construction of multiple PCR-gene chips for seven common food-borne pathogenic bacteria

Resumen de: CN121406803A

The invention establishes a method for simultaneously detecting vibrio parahaemolyticus, staphylococcus aureus, listeria monocytogenes, salmonella, shigella, bacillus cereus and vibrio alginolyticus on the basis of multiple PCR (Polymerase Chain Reaction) combined with a gene chip technology. The method comprises the following steps: selecting a heat-labile hemolysin gene of vibrio parahaemolyticus, a heat-resistant nuclease gene of staphylococcus aureus, a coded hemolysin O gene hlyA of listeria monocytogenes, an invasive protein A gene of salmonella enteritidis and an invasive plasmid antigen H gene of Shigella to carry out quintuple PCR (Polymerase Chain Reaction) amplification; bacillus cereus non-hemolytic toxin genes and vibrio alginolyticus similar Rpos sigma factor genes are selected for double PCR, two groups of multiplex PCR amplification products are hybridized with specific probes on the chip at the same time, and seven food-borne pathogenic bacteria can be detected at the same time. The method for detecting pathogenic bacteria by using the gene chip is high in specificity, high in sensitivity and strong in reliability.

Marine bioalkali biquaternary ammonium salt, and pharmaceutical composition and antibacterial application thereof

Resumen de: CN121405721A

The invention relates to the field of antibacterial agents, in particular to marine bioalkali biquaternary ammonium salt as well as a pharmaceutical composition and antibacterial application thereof. The novel biquaternary ammonium salt is constructed based on marine bioalkali eudistomin N through a pharmacophore splicing strategy, has a structural formula as shown in a formula I, has broad-spectrum antibacterial activity, is used for inhibiting growth of bacteria such as staphylococcus aureus, methicillin-resistant staphylococcus aureus, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa and salmonella enteritidis, and can be used for inhibiting growth of bacteria such as staphylococcus aureus, methicillin-resistant staphylococcus aureus, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa and salmonella enteritidis. The compound is particularly used for inhibiting escherichia coli ATCC 8739 or resisting klebsiella pneumoniae ATCC 10031.

Composite pharmaceutical composition for preventing and treating avian salmonella infection and application thereof

Nº publicación: CN121401341A 27/01/2026

Solicitante:

SHANDONG ZHONGYUE ANIMAL HUSBANDRY TECH CO LTD
\u5C71\u4E1C\u4F17\u8DC3\u755C\u7267\u79D1\u6280\u6709\u9650\u516C\u53F8

Resumen de: CN121401341A

The invention belongs to the field of veterinary drugs, and particularly relates to a composite pharmaceutical composition for preventing and treating avian salmonella infection and application of the composite pharmaceutical composition. The composition comprises the following components in parts by weight: florfenicol, coptis chinensis and schisandra chinensis extract, vitamin C and prebiotics. Florfenicol and specific traditional Chinese medicines are combined and matched with sufficient vitamin C and prebiotics to form an antibacterial-protection-repair three-in-one synergistic system. On the premise of remarkably reducing the dosage of florfenicol, the compound can achieve a better clinical curative effect, relieve toxic and side effects and promote rapid rehabilitation of infected poultry, has obvious comprehensive advantages and is suitable for preparing a medicine for preventing and treating avian salmonellosis.