Resumen de: CN121020835A
The invention discloses a method for improving livestock and poultry breeding wastewater treatment by a phycomycete system through starch wastewater and application, and belongs to the technical field of environmental engineering and biological recycling. The method comprises the following steps: (1) pretreating livestock and poultry breeding wastewater and starch wastewater to remove impurities; livestock and poultry breeding wastewater and starch wastewater are mixed according to a certain volume ratio to prepare mixed wastewater; (2) mixing chlorella sorojin and aspergillus oryzae in a logarithmic phase according to a certain quantity ratio, and inoculating to construct a microalgae-fungus co-culture system; and (3) putting the microalgae-fungus co-culture system into the mixed wastewater, treating under an illumination oscillation condition, and purifying the mixed wastewater. According to the method, the starch wastewater is adopted as a novel carbon source for replacing a traditional commercial carbon source, and a biological treatment mode of microalgae-fungus co-culture is combined, so that efficient purification of the livestock and poultry breeding wastewater is realized, meanwhile, biomass accumulation is promoted, resource utilization of wastes is realized, and dual targets of wastewater treatment and resource recovery are achieved.
Resumen de: CN121022548A
The invention discloses an intelligent sampling and on-line detection device and method for microalgae culture, and relates to the technical field of biological culture on-line detection. The microalgae stock solution detection unit comprises a stock solution detection pool, two sides of the stock solution detection pool are respectively provided with a divergent light source and a spectrum detection sensor, and the microalgae stock solution detection unit is used for detecting microalgae pigment and cell concentration in the stock solution through spectrum inversion; the microalgae diluent detection unit comprises a diluent detection pool, and a plurality of diluent detection sensors are arranged in the diluent detection pool and used for detecting the pH value, the conductivity and the inorganic salt ion concentration in the diluent; and the automatic sampling and diluting unit comprises a PLC (Programmable Logic Controller) as well as a first peristaltic pump, a second peristaltic pump, a first electromagnetic valve, a second electromagnetic valve and a plurality of liquid level meters which are electrically connected with the PLC, and is used for controlling linkage operation of each component and completing stock solution sampling, solution changing, stock solution detection pool cleaning, stock solution diluting, solution discharging and diluent detection pool cleaning. According to the invention, effective detection of multiple parameters of the microalgae culture syst
Resumen de: CN121020826A
The invention discloses a method for constructing an Algammox system, and relates to a method for constructing a system. The invention aims to solve the problems that the existing anaerobic ammonium oxidation bacteria (AnAOB) are easily damaged by ROS (reactive oxygen species) induced by light irradiation and are greatly influenced by DO (dissolved oxygen). According to the invention, microalgae is innovatively introduced into a PNA technology, and an Algammox system is constructed. Dissolved oxygen is supplied in situ by utilizing microalgae through photosynthesis, so that the dependence on mechanical aeration is reduced; meanwhile, a biofilm carrier strategy is adopted, so that the inhibition of illumination on AnAOB is effectively shielded, and the biomass retention capacity is enhanced. The result shows that the total nitrogen removal rate of the developed Algammox system is as high as 97%. The invention belongs to the technical field of biological environmental protection and biological energy.
Resumen de: CN121021241A
The invention discloses a production method of a microalgae water-soluble fertilizer based on swine urine. The production method comprises the following steps: S1, collecting swine urine; s2, primary treatment of swine urine; s3, carrying out mold removal and detoxification on the swine urine; s4, culturing the microalgae; s5, strengthening the microalgae; s6, collecting microalgae; s7, preparation of the water-soluble fertilizer: in the step S1, firstly filtering the pig urine by using a 100-150-mesh filter screen to remove feed residues or excrement particles, and in the step S2, firstly diluting the pig urine until the nitrogen content in the pig urine is 100-300mg/L, then carrying out catalytic ozonation on the pig urine, adding ferric oxide and activated carbon into the inner side of the pig urine according to a ratio of 2: 1, and uniformly stirring to obtain the water-soluble fertilizer. The ratio of the ferric oxide and the activated carbon to the swine urine is 1: 10, the microalgae water-soluble fertilizer with low heavy metal pollution is prepared by sufficiently treating the swine urine, then adjusting the microalgae and finally combining the swine urine with the microalgae, and the microalgae water-soluble fertilizer can be used in a farmland in a large area and is high in use efficiency.
Resumen de: CN121030277A
The invention provides an intelligent treatment method for mariculture tail water, and belongs to the technical field of machine learning. By constructing a multi-mode tail water environment-evolution data set, comprehensive perception of a tail water visual image sequence, physicochemical index time sequence data and operation control parameters is realized, and dynamic evolution prediction of a tail water system is carried out based on a virtual environment generation model. Through the steady state identification and mode mining module, the stable operation cluster, the critical boundary and the abrupt change mode of the tail water system can be quantified, control-abrupt change response knowledge is extracted, and a structured basis is provided for optimization decision making. On the basis, a multi-target control optimization model is constructed, efficient regulation and control over the microalgae growth rate, the suspended particle sedimentation rate and the nutritive salt absorption efficiency are achieved, meanwhile, the potential mutation risk is avoided, and steady-state operation of the system is guaranteed. According to the method, intelligentization, precision and multi-objective optimization of the tail water treatment process can be achieved, and efficient and reliable technical support is provided for mariculture tail water ecological management.
Resumen de: CN121022664A
The invention provides a cyanobacteria strain for producing phenylalanine and application of the cyanobacteria strain, and belongs to the technical field of functional cyanobacteria. The invention provides a cyanobacteria strain for producing phenylalanine, which is a synechocystis sp. Strain UV120, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.46456. The invention also provides a preparation method of the cyanobacteria strain for producing phenylalanine. According to the invention, two mutagenized blue algae strains are obtained by adopting ultraviolet mutagenesis, and the strain UV120 not only has a good growth characteristic under the condition of high-concentration L-phenylalanine or analogues thereof, but also has the characteristic of high yield of L-phenylalanine, so that a new means is provided for large-scale synthesis of phenylalanine.
Resumen de: CN121020840A
The invention discloses a method for improving the content of active oxygen by utilizing bacterium-alga co-culture and treating coking wastewater, and relates to the field of methods for improving the content of active oxygen by utilizing bacterium-alga co-culture and treating coking wastewater. The invention aims to solve the technical problem of high cost caused by the need of an external carbon source in the process of removing nitrogen and phosphorus by adopting microalgae at present. The method comprises the following steps: domesticating residual sludge by adopting different pollutants, and then mixing with microalgae for culturing; the cultured algal-bacterial symbiotic system is used for removing specific pollutants in coking wastewater, and the content of active oxygen in the system is measured after treatment. According to the method, the algal-bacterial symbiotic system is combined with the coking wastewater treatment process, so that the treatment cost and time are greatly reduced while the adaptability and the treatment efficiency of microorganisms to toxic and harmful substances are improved. The method is used for increasing the content of active oxygen and treating coking wastewater.
Resumen de: CN223607262U
The utility model discloses a microalgae flocculating settling harvesting device, and relates to the field of microalgae harvesting. A microalgae flocculating settling harvesting device comprises a settling pond and further comprises a conveying pipe fixedly inserted into a liquid inlet of the settling pond; the flocculating agent storage tank is placed on one side, close to the conveying pipe, of the sedimentation tank; the connecting pipe is fixedly inserted into the conveying pipe; according to the utility model, the flocculating agent is introduced by utilizing the negative pressure suction force generated by flowing of the microalgae solution in the conveying pipe, and the flocculating agent and the microalgae solution are mixed by virtue of the power of water flow, so that compared with the traditional mode of directly adding the flocculating agent into the sedimentation tank, the flocculating agent can be more quickly and uniformly dispersed in the microalgae solution, the mixing efficiency is obviously improved, and the energy consumption is reduced. The full proceeding of the flocculation reaction is promoted, the formation of a larger and denser flocculating constituent is facilitated, and the sedimentation effect of microalgae is improved.
Resumen de: CN223607249U
The utility model discloses a selective light-transmitting composite membrane photobioreactor which comprises a selective light-transmitting composite membrane cylinder body, an algae liquid inlet, a nutrient solution inlet and an exhaust port are formed in the top end of the selective light-transmitting composite film barrel; an air vent and a liquid outlet are formed in the bottom end of the selective light-transmitting composite film barrel; the selective light-transmitting composite film barrel body is of a three-layer structure; the outer layer is a light-transmitting hardened film layer, the middle layer is an additive film layer, and the inner layer is a food-grade packaging film layer. According to the utility model, the wall thickness of the cylinder is reduced, the light transmittance is increased, and the material cost is reduced; according to the utility model, the selective light-transmitting composite film barrel is arranged; after being used, the non-damaged paper can be repeatedly used after being sterilized; flow of microalgae is guaranteed through air, no mechanical power exists in the whole culture environment, the pollution risk caused by microalgae death does not exist, and the cleaning and maintenance rate is low.
Resumen de: CN121022596A
The invention discloses a culture medium for rapidly culturing chlorella, and relates to the technical field of microalgae biology, the culture medium comprises the following components: a nitrogen source: urea, a phosphorus source: dipotassium phosphate, a magnesium source: magnesium sulfate heptahydrate, an organic carbon source: molasses, and composite probiotics: the composite probiotics are composed of bacillus subtilis and lactobacillus acidophilus according to a viable count ratio of 5: 1; and the mineral regulating agent is zeolite powder. The compound probiotics secrete antibacterial substances to cooperate with the adsorption effect of the zeolite powder, so that the risk of infectious microbe pollution is reduced, residues are decomposed, the culture solution is clear for a long time and free of peculiar smell, and the pH fluctuation is small.
Resumen de: WO2025241022A1
The present invention provides a process for obtaining an extract with a high lutein content from microalgae, comprising a microalgae cultivation process with high biomass and lutein production, as well as a process for extracting lutein and chlorophylls using green solvents. The microalgae belong to the genus Chlorella from Brazilian biodiversity, which produces metabolites comprising lutein and chlorophylls. The cultivation process for the microalgae of the genus Chlorella was optimized, and different carbon and nitrogen sources used in the culture medium were evaluated with respect to lutein production. The lutein extraction process employs solvents such as ethanol and glycerin, which are safe for cosmetic and nutraceutical applications. The obtained extract has a high lutein content and also contains chlorophylls and neoxanthin. Lutein assists in protecting the skin against photoinduced damage, and the extract with a high lutein content is suitable for use in cosmetic compositions, pharmaceutical compositions, and the food industry.
Resumen de: WO2025243087A2
The present disclosure relates to compositions, nutraceutical compositions, and dietary supplements that improve gut health, enhance energy, improve focus and energy, reduce cravings, improve stress management, improve overall health, promote healthy aging, replenish nutrients, support immune health, promote healthy skin, promote hair and nail growth, and enhance recovery after physical activity. The compositions comprise one or more of each of seaweeds, enzymes, and protein; two or more of each of adaptogens, botanicals, extracts, fibers, microalgae, micronutrients, minerals, vitamins, and whole fruit or vegetable powders; and three or more probiotics.
Resumen de: WO2025243318A1
The present disclosure provides a microbiological process for the production of hydroquinone from benzoquinone in a microalgal system. The method is particularly characterized by the extraction of the hydroquinone product at a specific points in the process followed by re- introduction of the benzoquinone substrate into the microalgal system. The said method of the 0 present disclosure allows sustained production of hydroquinone.
Resumen de: CN121006355A
The invention relates to a microalgae photosynthetic carbon sequestration technology, and aims to provide a method for promoting microalgae carbon sequestration by a polyethylene glycol passivated nitrogen-doped carbon dot conversion spectrum. The method comprises the following steps: passivating the surfaces of nitrogen-doped carbon dots by using polyethylene glycol, and then directly adding the passivated nitrogen-doped carbon dots into a microalgae suspension, ultraviolet light with the wavelength of 280-390 nm in external light is converted into blue light with the wavelength of 400-500 nm by passivating the nitrogen-doped carbon dots so as to be directly utilized by microalgae, so that growth and carbon sequestration are promoted while the stress resistance of algae cells is improved. According to the method, damage to algae cells can be greatly reduced by converting an ultraviolet spectrum, and meanwhile, microalgae growth and carbon sequestration can be further promoted by converting ultraviolet light into blue light; the spectrum conversion effect is enhanced, the overall light energy utilization efficiency of microalgae carbon sequestration is improved, light inhibition is relieved, and the stress resistance of microalgae cells is improved, so that microalgae growth carbon sequestration is promoted, and the development of the microalgae carbon sequestration industry is promoted.
Resumen de: CN121003297A
The invention discloses a preparation method of a spirulina-fish oil nano-emulsion and application of the spirulina-fish oil nano-emulsion in fish oil microcapsules, and belongs to the technical field of microalgae application, and the preparation method specifically comprises the following steps: adding spirulina powder into pure water, and magnetically stirring uniformly to obtain a spirulina suspension; dropwise adding fish oil into the spirulina turbid liquid, and pre-emulsifying by using a high-speed dispersion homogenizer during the period to prepare a spirulina fish oil crude emulsion; treating the crude spirulina fish oil emulsion by using a high-pressure micro-jet homogenizer to obtain the spirulina-fish oil nano-emulsion. And carrying out spray drying on the spirulina-fish oil nano-emulsion by using a spray drying technology to obtain the spirulina-fish oil microcapsule. The spirulina fish oil microcapsule prepared by the invention has relatively good oxidation stability and thermal stability, can well cover the fishy smell of the fish oil, is easy to transport and store, and is more easily accepted by consumers.
Resumen de: CN121006354A
The invention relates to a microalgae carbon sequestration technology, and aims to provide a method for promoting microalgae carbon sequestration by using a transparent film carrying a nitrogen-doped carbon dot coating. According to the method, a transparent film is arranged on the outer side of the photobioreactor in a surrounding manner, so that external light rays penetrate through the transparent film and then enter the photobioreactor; the nitrogen-doped carbon dot coating is carried on the surface of the transparent film, ultraviolet light of 280-390 nm in external light is converted into blue light of 400-500 nm through the coating, and growth and carbon sequestration are promoted while damage to algae cells is reduced. Damage to algae cells can be greatly reduced by converting ultraviolet rays, and meanwhile, the ultraviolet rays are converted into blue light to further promote microalgae growth and carbon sequestration. The light source improvement effect of the photobioreactor can be comprehensively improved, the entering blue light source is enhanced and more uniform, and spectral distribution is optimized; the method can be used for manufacturing the composite film in an industrial production scale and transforming the photobioreactor in batches, and is low in cost, simple to operate and suitable for popularization and application.
Resumen de: CN121005766A
The invention discloses an application of a transcription factor ChZF7 of a basophilic microalgae Chlorella sp. BLD in regulation and control of salt tolerance of microalgae. According to the invention, the coding gene of the transcription factor ChZF7 is transferred into chlamydomonas reinhardtii by using a transgenic technology after intron insertion and codon optimization, so that transgenic chlamydomonas reinhardtii is obtained. The salt tolerance of the chlamydomonas reinhardtii with the over-expressed ChZF7 gene is remarkably improved, the accumulation of neutral fat and active oxygen under the stress of 225mM salt is less than that of the chlamydomonas reinhardtii in a control group, the content of penetrating substance proline is increased, in addition, the change of cell components in the chlamydomonas reinhardtii under the stress of salt can be weakened by the over-expressed ChZF7 gene, and the salt tolerance of the chlamydomonas reinhardtii is improved. The transcription factor ChZF7 and the gene coded by the transcription factor ChZF7 can regulate and control the salt tolerance of the chlamydomonas reinhardtii, a foundation is laid for genetic improvement and directed molecular breeding of the microalgae, the transcription factor ChZF7 plays an important role in improvement of the salt tolerance of the microalgae, reutilization of salinized water resources is facilitated, and the transcription factor ChZF7 and the gene coded by the transcription factor ChZF7 have
Resumen de: CN120988847A
The invention belongs to the technical field of microalgae culture, and particularly provides an improved culture solution for haematococcus pluvialis culture and a preparation method thereof. The culture solution is prepared from 1.5 to 2.5 mg/L of alpha-cyclodextrin embedded sodium acetate, 0.5 to 0.5 mg/L of cobalt nitrate hexahydrate, 0.05 to 1 mg/L of copper sulfate, 0.3 to 0.9 mg/L of zinc sulfate heptahydrate, 1.5 to 2.5 mg/L of manganese chloride tetrahydrate, 3 to 7 mg/L of boric acid, 8 to 20 mg/L of sodium carbonate, 10.0 to 20.0 mg/L of disodium hydrogen phosphate dihydrate, 1200 to 1400 mg/L of sodium nitrate, 0.5 to 3 mg/L of EDTA (Ethylene Diamine Tetraacetic Acid), 20 to 50 mg/L of calcium chloride, 70 to 100 mg/L of magnesium sulfate heptahydrate, 50 to 80 mg/L of monopotassium phosphate trihydrate, 0.5 to 1.0 mg/L of ferrous sulfate heptahydrate and 0.01 to 0.03 mg/L of ammonium molybdate tetrahydrate. According to the invention, alpha-cyclodextrin is introduced to embed sodium acetate, ferrous sulfate heptahydrate and ammonium molybdate tetrahydrate are added, and a sodium carbonate-disodium hydrogen phosphate-potassium dihydrogen phosphate composite buffer system is constructed, so that the problem of easy imbalance of traditional carbon source supply is solved, and the defect of single and short elements of a traditional formula can be overcome; pH fluctuation caused by cell metabolism acid production during high-density culture can be effectively coped w
Resumen de: CN223576486U
The utility model belongs to the technical field of industrial culture of microalgae, and particularly relates to a sampling device in industrial culture of selenium-enriched microalgae, which comprises a culture container, a mounting seat, a clamping and limiting component, an anti-skid guide component, an elastic telescopic pipe, an end cover, a sampling hose and a reset component, the clamping and limiting assembly is arranged on the mounting base, the mounting base is detachably connected with the breeding container through the clamping and limiting assembly, and the sampling hose is connected with the mounting base through the anti-skid guide assembly. The sampling device is reasonable in design, compact in structure and simple and convenient to operate, the stability and reliability of sampling are improved, stable connection with a culture container can be ensured, displacement caused by vibration or external force is prevented, meanwhile, the hose can be prevented from slipping and excessively bending, the smoothness of the sampling process and the integrity of a sample are ensured, and the sampling efficiency is improved. The device is convenient to maintain, disassemble, assemble and replace, the contamination risk of cultures can be reduced, and the effects of simplifying the operation process and improving the working efficiency can be achieved.
Resumen de: CN120997315A
The invention discloses a microalgae density measurement method based on image color correction, which comprises the following steps: providing stable illumination by using an LED panel light source under a pure white background, collecting images of microalgae samples with different densities, and constructing a color difference-density model of microalgae images; extracting the RGB average value of the whole image and calculating the weighted combination chromatic aberration of the RGB average value and the ideal white color; the method comprises the following steps: selecting a plurality of pure color backgrounds with different colors and different materials, collecting microalgae sample images, and setting a zero-density microalgae sample as a reference standard for correcting system errors; the image is processed through a color correction algorithm, chromatic aberration offset caused by the background is compensated, and the microalgae image approximates to the expression under the pure white background in a color space; according to the method, the influence of background color on the microalgae image can be corrected, the detection process is simplified, the accuracy and reliability of microalgae density measurement are improved, and the method is suitable for microalgae density monitoring under various environmental conditions.
Resumen de: CN120988846A
The invention discloses a culture method and application of beta-1, 3-glucan-producing microalgae, and realizes high-efficiency synergy from microalgae culture to separation. According to the enrichment culture medium, by optimizing the ratio of carbon and nitrogen sources and nutritional ingredients, efficient accumulation of beta-1, 3-glucan can be remarkably improved, meanwhile, the microalgae biomass is promoted, and the problem of low product content in conventional culture is solved. The matched microalgae culture method breaks through the limitation that traditional plate streak bacterial colonies are mixed, water drop separation consumes time and is prone to pollution, the high-purity single strain is accurately extracted from a complex water sample, the operation standardization degree is high, and the manual randomness influence is reduced. Through the synergistic application of the two, the microalgae with high biomass and high glucan content can be efficiently obtained, the separation purity can be guaranteed, high-quality resources are provided for scientific research and industrial production, and the application of the microalgae in the fields of food, medicine and the like is promoted.
Resumen de: CN120988056A
The invention discloses a preparation method of a chlamydomonas reinhardtii polypeptide composition, and belongs to the technical field of natural active ingredients. The preparation method comprises the following steps: respectively carrying out nitrogen deficiency stress culture on chlamydomonas reinhardtii cc620 and cc621 strains, mixing, standing for mating, and centrifugally collecting mating supernate; adding the mating supernatant into the chlamydomonas reinhardtii fresh cell suspension, after cell wall hydrolysis removal, centrifugally collecting the supernatant to obtain cell wall hydrolysate, and resuscitating and culturing the protoplast precipitate to be circularly used for cell wall hydrolysis removal; the cell wall hydrolysate is subjected to nanofiltration, concentration and impurity removal are achieved, and the chlamydomonas reinhardtii polypeptide composition can be obtained. The chlamydomonas reinhardtii polypeptide composition prepared by the invention is a product rich in hydroxyproline and arabinose modified oligopeptide, and shows relatively high bioavailability in a rat oral pharmacokinetic test. The potential application of the microalgae-derived collagen oligopeptide analogue provided by the invention relates to the fields of foods, health foods, cosmetics, medicines and the like.
Resumen de: CN120987475A
The invention relates to a microalgae bioreactor and a sewage treatment method. The microalgae bioreactor comprises a shell, a filter bed, an LED light source and a spraying device, a water outlet and a reflux inlet are formed in the lower part of the shell; the filter bed is made of a porous material and is arranged in the shell; the number of the LED light sources is at least one, and the LED light sources are arranged outside and/or inside the shell; the spraying device is arranged above the filter bed; a water inlet connected with a sewage inlet pipeline is formed in the spraying device; the water inlet is also connected with the reflux inlet through a reflux pipeline. According to the microalgae bioreactor disclosed by the invention, nitrogen and phosphorus substances in sewage are effectively removed by utilizing a microalgae biological membrane, and the effect of recycling nitrogen and phosphorus nutrient elements is realized.
Resumen de: CN120989069A
The invention provides a preparation method of microalgae gel balls for water treatment, and relates to the field of water treatment, and the preparation method comprises the following steps: step S1, preparing a composite suspension, step S2, carrying out primary crosslinking to form balls, step S3, carrying out secondary crosslinking, step S4, carrying out in-situ enhancement, and step S5, coating. According to the method, microalgae is subjected to compound modification to obtain gel beads, and the finished product gel beads are obtained through coating, so that the swelling resistance and mechanical strength of the gel beads are improved, and the situation that a compact gel network and a multi-layer coating structure hinder nutrient substances, carbon dioxide and the like in a water body from diffusing to microalgae cells is avoided; the photosynthesis efficiency and pollution degradation capability of the microalgae are ensured, and the water treatment efficiency of the microalgae is improved.
Nº publicación: CN120988812A 21/11/2025
Solicitante:
YUNNAN ALPHY BIOTECH CO LTD
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Resumen de: CN120988812A
The invention relates to the technical field of microalgae culture, in particular to an air supply and temperature regulation system of a floating microalgae photobioreactor and application of the air supply and temperature regulation system, the air supply and temperature regulation system comprises a floating platform, a plurality of incubators are arranged in the floating platform, an air supply system is arranged among the incubators, and a driving mechanism is further arranged above the floating platform. According to the air supply and temperature regulation system of the floating microalgae photobioreactor and the application thereof, the air bag is inflated and deflated to drive overall floating and sinking, stability and reliability of floating and sinking actions are ensured by utilizing the characteristic of high corrosion resistance of the air bag, and an inserting rod in a limiting pipe drives a round rod to rotate through matching of a convex block and a spiral groove in the fixed-distance rising process; therefore, the three groups of limiting blocks are controlled to pop out in sequence, three different mechanical limiting points are formed, and the multi-stage accurate control of the immersion depth of the incubator is realized.