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Recombinant algal strain for expressing glycolate oxidase, catalase, glyoxylate polyaldolase and hydroxymalonate semialdehyde reductase as well as construction method and application of recombinant algal strain

Resumen de: CN120290328A

The invention provides a recombinant algal strain for expressing glycollate oxidase, catalase, glyoxylate polyaldolase and hydroxymalonate semialdehyde reductase as well as a construction method and application of the recombinant algal strain, and belongs to the technical field of genetic engineering. The invention provides a recombinant microalgae strain, which is a microalgae strain for expressing glycollic acid oxidase, catalase, glyoxylic acid polyaldolase and hydroxymalonic semialdehyde reductase in chloroplast. The recombinant microalgae strain can decompose a light respiration by-product glycollic acid into carbon dioxide, the carbon dioxide concentration of chloroplast is improved, the carbon sequestration efficiency of microalgae is effectively improved, and fixed CO2 can be converted into biomass, pyruvic acid, starch, grease and other metabolites and bioactive substances. The recombinant microalgae strain disclosed by the invention has a wide application prospect in the aspects of biological carbon sequestration, synthesis of microalgae metabolites and preparation of microalgae bioactive substances.

Cyanobacteria SCSIO17256 with high exopolysaccharide yield and application of cyanobacteria SCSIO17256 in erosion resistance of coral island

Resumen de: CN120290399A

The invention discloses cyanobacteria SCSIO17256 with high yield of exopolysaccharides and application of the cyanobacteria SCSIO17256 in erosion resistance of coral islands. The cyanobacteria is named as cyanobacteria Oculatus sp.SCSIO17256, and the preservation number of the cyanobacteria Oculatus sp.SCSIO17256 is CCTCC (China Center For Type Culture Collection) NO: M2025524. The cyanobacteria Ocularia sp.SCSIO17256 provided by the invention can effectively promote the formation of calcareous sand biological crust, has relatively strong sand consolidation capability and extremely strong anti-shearing capability, and can be applied to prevention and treatment of water and soil loss of coral islands and construction of coral island calcareous sand biological soil crust. Compared with physical sand consolidation, chemical sand consolidation and vegetation cultivation, microorganisms are used as a novel sand consolidation and crust cultivation mode, and the method has the advantages of being high in adaptability, low in cost, quick in effect and the like.

Method for improving stability of steel slag by inducing CO2 through microalgae culture

Resumen de: CN120289109A

The invention provides a method for improving stability of steel slag by inducing CO2 through microalgae culture, and relates to the technical field of steel slag reutilization. The method comprises the following specific steps: S1, crushing and screening steel slag to obtain steel slag particles; s2, adding concentrated salt wastewater into the steel slag particles, and stirring to obtain steel slag slurry; s3, placing the steel slag slurry in a microalgae culture device, introducing CO2 gas, stirring, carrying out induced carbonization reaction by using microorganisms, and carrying out solid-liquid separation to obtain calcium and magnesium removed steel slag precipitate and microalgae turbid liquid; s4, using the calcium and magnesium removed steel slag as machine-made sand; s5, using the microalgae turbid liquid for culturing shellfish and economic fish; and S6, filtering out residual shells eaten by the economic fishes through a filter screen, and using the shells as biological calcium magnesium carbonate. Through microalgae culture and carbonization reaction, the complex active free calcium and magnesium oxide conversion process is simplified by utilizing a biological process, so that the steel slag is suitable for concrete production. The stability of the steel slag and CO2 resource utilization are improved, and remarkable environmental and economic benefits are achieved.

Composite porous phosphorus removal filter material and preparation method thereof

Resumen de: CN120290543A

The invention discloses a composite porous phosphorus removal filter material and a preparation method thereof, the filter material is prepared by taking aluminum hydroxide, limestone, concrete, perlite, modified zeolite, a foaming agent and a mixture of euglena, chlorella and spirulina as main components through the steps of pulping, foaming, coating, curing, crushing, screening and the like. The phosphorus removal efficiency is remarkably improved through ion exchange and chemical adsorption effects of the modified zeolite, the performance of the filter material is further enhanced through biological adsorption and biological assimilation effects of the microalgae, and in addition, the phosphorus removal effect of the filter material is further improved through stable precipitates formed by special components and phosphate. The filter material has the advantages of efficient phosphorus removal, environmental protection, no pollution, multifunctionality, economical efficiency and the like, is suitable for the fields of sewage treatment plants, lake remediation, aquaculture and the like, and has the advantages of high phosphorus removal efficiency, simple preparation process, low raw material cost and easiness in industrial production.

USE OF MICROALGAE AND MICROALGAE DERIVED COMPONENTS IN CULTIVATED FOOD PRODUCTION

Resumen de: AU2023416213A1

Provided herein are methods for cultivated food production involving the use of microalgae. The methods include recycling spent cell media that are used to cultivate cells for cultivated food production as well as enriching culture medium used to cultivate the same cells. The microalgae are used to recycle the spent culture medium. The microalgae may be used to extract microalgae derived components to enrich the culture medium. The algae recycling and algae enriched growth media as provided herein may be used with any animal cell culture. In some variations, the microalgae or microalgae derived components may be used as a raw ingredient in the preparation of cell based seafood products. The methods provided herein may be used for the production of seafood products. The methods herein may provide at least one added advantage—the use of algae may add nutrients, flavors, and aromas typically desired in seafood.

MODIFIED MICROALGAE FOR ENHANCED PHOSPHATE UPTADE INVOLVING OVEREXPRESSION OF PSR1 AND OPTIONALLY UNDEREXPRESSION OF PTC1

Resumen de: US2025223542A1

The invention provides a recombinant microalgal strain comprising in its genome a first modification which causes overexpression of a PSR1 gene, and optionally a further modification which reduces or eliminates expression from an endogenous PTC1 gene. The strains of the invention have utility in promoting phosphate uptake, for example from wastewater, with the microalgae then being useful as fertilisers.

Method for promoting microalgae carbon sequestration and oil production by using plant hormones and salt-containing wastewater

Resumen de: CN120272319A

The invention belongs to the technical field of microalgae culture, and discloses a method for promoting microalgae carbon sequestration and oil production by using plant hormones and salt-containing wastewater, microalgae are cultured in a wastewater culture medium added with sodium chloride and the plant hormones, the plant hormones at least comprise abscisic acid, and the abscisic acid can effectively relieve salt stress and promote microalgae oil accumulation and growth. The plant hormone further comprises one of auxin and gibberellin, and the abscisic acid and the auxin or the gibberellin have a synergistic effect, so that the grease yield of the microalgae is more favorably improved. In addition, sodium chloride, abscisic acid, 4-amino-2-methylthiopyrimidine-5-carboxamide and 4-methyl-5-thiazoleethanol can also be added into the wastewater, so that the biological yield of the microalgae and the grease accumulation can be synergistically enhanced. The invention provides an effective way for reducing the microalgae culture cost and realizing the industrial production of microalgae biodiesel, and has both environmental and energy benefits.

Artificial bait additive for improving intestinal flora of yellow-fin tuna and preparation method of artificial bait additive

Resumen de: CN120266971A

The invention discloses an artificial bait additive for improving intestinal flora of yellow fin tuna, which comprises 15-30% of fish meal, 15-30% of soybean meal, 5-10% of wheat starch, 2-5% of fish oil, 1-3% of rapeseed oil, 5-10% of mulberry leaf powder, 5-15% of fly maggot powder, 1-5% of microalgae, 0.1-0.5% of bile acid, 1-3% of pine needle meal, 5-15% of brown rice and 1-5% of cow milk casein. The Chinese herbal medicine active components comprise 0.01%-0.1% of an acanthopanax senticosus extract, 0.05%-0.5% of ophiopogon japonicus polysaccharide, 1%-5% of bitter gourd dry powder, 0.01%-0.1% of a platycodin extract and 1%-5% of a fresh peach leaf fermentation product; the probiotic complexing agent comprises the following components in percentage by weight: 0.01%-0.5% of bacillus subtilis fermentation liquor, 0.01%-0.2% of lactobacillus acidophilus freeze-dried powder and 0.01%-0.3% of bacillus licheniformis fermentation liquor, and the auxiliary components comprise 1%-5% of a brewer's grain fermentation substrate, 0.1%-0.5% of a fish oil emulsifier and 0.1%-1% of fructo-oligosaccharide. According to the present invention, the growth performance of the yellow-fin tuna can be comprehensively promoted, the immunity is enhanced, the intestinal health of the yellow-fin tuna is optimized, and the feed conversion rate can be increased.

Method for improving heat resistance of microalgae

Resumen de: CN120272318A

The invention relates to a method for improving the heat resistance of microalgae by adding phospholipid or alcohol amine solvents, which comprises the following steps: selecting separated and purified microalgae, inoculating a single algae colony of the microalgae to a BG11 liquid culture medium, respectively preparing phospholipid or alcohol amine solvents of different types and different concentrations, and selecting the optimal treatment concentration and the appropriate solvent type; grouping the algae liquid, adding phospholipid or alcohol amine solvents with the corresponding optimal concentration into an experimental group, and not adding phospholipid or alcohol amine solvents into a control group; after high-temperature stress, comparing the two groups of growth rates, cell survival rates and various physiological indexes; results show that the heat resistance of the algal strain treated by the phospholipid or alcamines solvent is obviously improved, and the heat resistance shows that the cell survival rate is improved, lipid peroxidation is reduced, the antioxidant enzyme activity is enhanced, and the proline content is increased; the method is simple to operate and low in cost, and an effective solution is provided for large-scale culture and application of microalgae in a high-temperature environment.

Sludge dewatering waste liquid treatment method based on microalgae semi-continuous culture mode

Resumen de: CN120271163A

The invention discloses a sludge dewatering waste liquid treatment method based on a microalgae semi-continuous culture mode. The sludge dewatering waste liquid treatment method comprises the steps of pretreatment, waste liquid treatment through microalgae semi-continuous culture and post-treatment. According to the method, water purification of sludge dewatering waste liquid and recovery of nitrogen and phosphorus resources can be effectively achieved by means of growth and metabolism characteristics of microalgae, the problem that nitrogen and phosphorus loads are increased due to direct backflow of wastewater at the present stage is solved, meanwhile, CO2 is fixed through photosynthesis of the microalgae, external carbon sources do not need to be supplemented, and compared with a traditional biological treatment process, the method has the advantage that the cost is low. Adding of chemicals in the treatment process and carbon emission in the treatment process can be effectively reduced, energy consumption is low, and the treatment effect is good.

Method for improving carbon sequestration capacity of nannochloropsis oculata by domesticating and regulating key enzyme with high ammonium salt

Resumen de: CN120272395A

The invention relates to a biomass energy utilization and resource utilization technology, and aims to provide a method for improving the carbon sequestration capability of nannochloropsis oculata by domesticating and regulating a key enzyme with high ammonium salt. The method comprises the following steps: carrying out nitrogen salt gradient domestication on nannochloropsis oculata cells, and regulating and controlling the expression quantity of five key enzymes, namely H < + > transport ATP enzyme, glutamine synthetase, asparagine synthetase, carbonic anhydrase and ribulose-1, 5-diphosphate carboxylase/oxygenase, in the nannochloropsis oculata cells; the domesticated and cultured nannochloropsis oculata can independently use coked ammonium sulfate as a nitrogen source required by growth; the domesticated algal strains are subjected to transfer culture, and verification and screening are carried out aiming at the capability of fixing CO2 in flue gas and the prevention of over-acidification of the culture medium. According to the method, the utilization of coked ammonium sulfate by the nannochloropsis oculata can be enhanced without causing over-acidification of the culture medium, the microalgae culture cost can be effectively saved, and the flue gas CO2 immobilization capability of the nannochloropsis oculata is improved; a new thought is provided for large-scale breeding and cultivation by using coked ammonium sulfate and improvement of economic efficiency.

Microalgae culture device

Resumen de: KR20250103434A

미세조류 배양장치가 개시된다. 본 발명의 일 측면에 따른 미세조류 배양장치는 미세조류가 배양되는 배양액을 수용하고, 내부에 경사진 바닥면을 구비하는 수조; 및 상기 배양액을 상기 경사진 바닥면의 하부 영역에서 상기 경사진 바닥면의 상부 영역으로 이송시키는 배양액 이송부를 포함할 수 있다.

A RIVER OR MARINE PHOTOBIOREACTOR-MIMICKING MICROALGAE CULTIVATION DEVICE

Resumen de: KR20250103521A

본원은 제 1 배양액을 포함하는 배양조; 상기 배양조 내에 배치되고, 미세조류를 포함하는 제 2 배양액을 저장하는 복수의 광화학 배양기; 및 상기 배양조에 이산화탄소를 주입하는 이산화탄소 주입부를 포함하고, 상기 광화학 배양기는 하부에 방오효과를 갖는 멤브레인을 포함하는 것인, 미세조류 배양 장치에 관한 것이다.

Preparation method and application of zinc-rich hot spring algae extract

Resumen de: CN120241544A

The invention relates to a preparation method and application of a zinc-rich hot spring algae extract, and belongs to the technical field of microalgae. Zinc-containing habitat water is used as a culture medium water source to carry out zinc-rich culture on the hot spring algae; subsequently, efficiently extracting trace elements such as zinc, strontium and the like in the microalgae through a carbonization combined bionic extraction technology; no organic solvent is used in the whole extraction process, so that the environmental burden is small. The obtained zinc-rich hot spring algae extract can be applied to skin care products for repairing, resisting aging and controlling oil.

Application of electron transport protein element group displayed on cell surface and biological photovoltaic device constructed by electron transport protein element group

Resumen de: CN120249062A

The invention relates to an application of an electron transport protein element group displayed on the surface of a microbial cell in construction of biological photovoltaic, and a biological photovoltaic device, a cathode tank of which comprises microalgae capable of secreting intermediate energy metabolites and a culture medium thereof, and a set of electron transport protein elements that oxidizes the intermediate energy metabolite and releases and transports electrons. According to the invention, microalgae capable of efficiently secreting an intermediate energy metabolite to the outside of a cell is adopted as a light energy capturing body, so that the energy metabolite is transferred from the inside of the cell to the outside of the cell, the light energy conversion efficiency is improved, the efficient transmembrane transfer of an energy substance is realized, and the protein extracellular display technology is adopted. The electron transfer protein elements are anchored on the surfaces of the cells, so that the loss caused by aggregation among the electron transfer protein elements and intracellular metabolism of heterotrophic microorganisms is avoided, the oxidation efficiency and electron transfer efficiency of intermediate energy metabolites are improved, and the energy output of the biological photovoltaic device is improved.

Tubular photo-biological reaction system and method for RO (Reverse Osmosis) concentrated water and flue gas treatment

Resumen de: CN120247271A

The invention discloses a tubular photo-biological reaction system and a tubular photo-biological reaction method for RO (Reverse Osmosis) concentrated water and flue gas treatment. According to the tubular photo-biological reaction system or method for RO concentrated water and flue gas treatment, provided by the invention, the RO concentrated water and flue gas CO2 can be synchronously treated by utilizing microalgae for treatment, and the dual purposes of efficient carbon sequestration and pollutant removal are achieved. The microalgae for treatment in the transparent reaction tube absorbs CO2 in the flue gas through photosynthesis under the action of illumination and converts the CO2 into biomass, so that the concentration of CO2 in the flue gas can be remarkably reduced; meanwhile, the microalgae for treatment can promote self growth by utilizing nutrient substances such as nitrogen and phosphorus in the strong brine, and harmful substances such as COD (Chemical Oxygen Demand), TN (Total Nitrogen) and TP (Total Phosphorus) in the strong brine are effectively degraded. By means of the synchronous treatment mode, CO2 emission is reduced, and a green and sustainable pollution treatment scheme can be provided for steel plants.

Vegetable-algae symbiosis method based on fish manure concentrated clear liquid

Resumen de: CN120247266A

The invention discloses a fish manure concentrated clear liquid-based vegetable-algae symbiosis method, which is characterized in that diluted fish manure concentrated clear liquid is placed in a transparent planting tank, the concentration of the diluted fish manure concentrated clear liquid is 25-50% of the concentration of the original fish manure concentrated clear liquid, chlorella pyrenoidosa is cultured in the planting tank, and meanwhile, the chlorella pyrenoidosa is cultured in the planting tank. Leaf vegetables are planted in the planting grooves through floating plates, planting cups for planting the leaf vegetables are arranged on the floating plates, and the leaf vegetables are planted in the planting cups. A culture wastewater microalgae treatment system and a vegetable water culture system are coupled to construct a vegetable-algae symbiotic system, oxygen is supplied to vegetable roots through O2 generated by microalgae photosynthesis, vegetable growth is promoted, and nutrient absorption efficiency is improved. Finally, the purpose of reducing the concentration of pollutants in the fish manure concentrated clear liquid is achieved, meanwhile, vegetables are produced, and the purpose of resource utilization of waste of the recirculating aquaculture system is achieved.

METHOD FOR PRODUCING HIGH YIELD DESIRED PRODUCTS FROM MICROALGAE

Resumen de: KR20250100862A

본 발명은 미세조류로부터 관심 생성물을 고수율로 수득하기 위한 방법에 관한 것으로, 더욱 상세하게는 (a) 고정 탄소원으로서 유기산을, 질소원으로서 요소를 포함하는 배지를 조성하는 단계; (b) 상기 조성된 배지에 관심 생성물의 생성을 위한 미세 조류 세포를 제공하는 단계; (c) 상기 미세 조류 세포가 제공된 배지를 암흑의 호기성 종속영양 발효 조건하에 유가식 영양 공급에 의해 미세 조류를 배양하는 단계; (d) 상기 배양 중 세포 분화를 거치기 전 영양생장기에서 배지와 세포를 분리하는 단계; (e) 상기 분리한 세포로부터 관심 생성물을 정제하는 단계;를 포함하되, 상기 미세 조류 세포는 종속영양생물인 해마토코쿠스 라커스트리스(Haematococcus lacustris)인 것을 특징으로 한다. 본 발명에 의하면, 간단한 공정으로 관심 생성물을 단시간 내 고수율로 수득할 수 있어, 운용 비용을 절감하고, 대규모 경제적 생산으로 더 큰 시장에 대한 산업적 접근이 가능토록 한다는 장점이 있다.

METHOD OF EFFICIENT CITRAMALATE PRODUCTION FROM BROWN MACROALGAE BIOMASS BY ENGINEERED VIBRIO SP. DHG

Resumen de: US2025215387A1

The present disclosure relates to efficient production of citramalate, which is a valuable industrial compound, from brown microalgae by using a transformed Vibrio sp. DHG strain. According to the present disclosure, it is possible to efficiently produce citramalate, which is used in various industrial fields, from brown microalgae-based raw materials by using a recombinant microorganism for producing citramalate. Therefore, the present disclosure not only introduces an innovative source for citramalate to advance the field, but also lays the foundation for extensive and efficient applications in bio-based industries.

STRUCTURE AND METHOD FOR PROMOTING MICROALGAE GROWTH

Resumen de: US2025212852A1

An artificial structure for promoting microalgae growth includes a 3D-printed structure formed by positioning a printing surface on a movable stage of a 3D bioprinter in contact with a bio-ink that includes a mixture of a pre-polymer material with one or more of cellulose-derived nanocrystals (CNC), and microalgae cells. By projecting modulated light onto the printing surface while moving the stage, the bio-ink is progressively polymerized to define layers of an artificial coral structure with microalgae cells disposed thereon, where the artificial coral structure is configured to scatter light within the structure.

COMPOSITION CONTAINING EXTRACELLULAR VESICLES DERIVED FROM MICROALGAE

Resumen de: WO2025142932A1

The purpose of the present invention is to provide a composition that contains extracellular vesicles derived from microalgae and has suppressed discoloration.　In one embodiment of the present invention, a composition containing (A) extracellular vesicles derived from microalgae, (B) a polyhydric alcohol, and (C) at least one chemical selected from the group consisting of organic acids, salts thereof, and nonionic surfactants, is prepared.

COMPOSITION CONTAINING EXTRACELLULAR VESICLES DERIVED FROM MICROALGAE

Resumen de: WO2025142924A1

The purpose of the present invention is to provide a composition containing extracellular vesicles derived from microalgae. In one embodiment of the present invention, the composition contains (A) extracellular vesicles derived from microalgae, and, optionally, one or more chemicals selected from the group consisting of (B-1) to (B-3), where: (B-1) is one or more chemicals selected from the group consisting of primary alcohols and polyhydric alcohols, (B-2) is one or more chemicals selected from the group consisting of organic acids and salts thereof, and (B-3) is a chelating agent.

LIPID PRODUCING MARINE MICROALGA

Resumen de: WO2024132882A1

The invention provides marine microalgal strain of the Ulvales order of the Ulvophyceae class which have utility in the efficient and environmentally beneficial preparation of triacylglycerides and polyunsaturated fatty acids, which can in turn be used for fuels or feeds.

INNER LIGHT CLEANING DEVICE FOR MICROALGAE CULTURE

Resumen de: KR20250098733A

본 발명은 수조내 장착되는 봉상의 내부광을 세척하기 위한 것으로, 노즐이 내주면에 장착된 말굽형 세척부; 상기 말굽형 세척부의 일단에 장착된 세척지지부; 및 상기 세척지지부의 단부를 지지하되, 상기 세척지지부에 세척액을 공급하는 세척액공급관이 장착된 세척액공급부를 포함하는 미세조류 배양용 내부광 세척장치를 제공한다.

INNER LIGHT CLEANING DEVICE FOR MICROALGAE CULTURE

Nº publicación: KR20250098735A 01/07/2025

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