Resumen de: CN120349852A
The invention discloses a device and a method for efficient enrichment and slow release application of carbon dioxide, and belongs to the field of seaweed and microalgae cultivation. A device for efficient enrichment and slow release application of carbon dioxide comprises a cultivation box and a mounting ring mounted on the cultivation box, and further comprises an aeration pipe arranged in the cultivation box and provided with a plurality of groups of aeration holes; the at least two groups of communicating pieces are arranged on the symmetrical outer side walls of the cultivation box, the communicating piece on one side is communicated with a water inlet pipe, and the communicating piece on the other side is communicated with a water drainage pipe; the connecting shaft is rotationally connected to the side, close to the water inlet pipe, in the cultivation box, and a water wheel is fixedly connected to the connecting shaft; the adding hopper communicates with the side, close to the water wheel, of the cultivation box, and a discharging assembly is arranged in the adding hopper; the invention can overcome the problems of non-uniform distribution of nutrient substances in water, low utilization rate of algae and easy damage of algae.
Resumen de: CN120349890A
The invention relates to a white chlorella pyrenoidosa mutant strain and application thereof, and belongs to the technical field of microalgae biology. The chlorella pyrenoidosa mutant strain is named as YYZM024, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.40787, and the preservation date is September 19, 2023. The mutant strain is cultured in a glucose-containing heterotrophic culture medium, and after the mutant strain is cultured under the dark condition of 25-28 DEG C for 7-10 days, the content of chlorophyll in cells is still stably maintained at a relatively low level, and the color characterization is very stable. The chlorophyll content of the mutant strain is not obviously changed after passage inoculation for six months, and dry algae powder is always milky white, so that the genetic character of the mutant strain is relatively stable, and the mutant strain can be used as an algae species for large-scale production of algae source protein or an algae powder source of food additives.
Resumen de: CN120350003A
The invention discloses a micromolecule RNA AsrR9 and application thereof in regulation and control of ammonium salt stress tolerance of synechocystis PCC6803, and belongs to the field of industrial microorganisms. According to the invention, AsrR9 is subjected to overexpression, such that an overexpressed algae strain OEAsrR9 is obtained. In a BG11 solid culture medium, the growth state of the OEAsrR9 in the range of 30 mM to 120 mM is obviously superior to that of WT. In a BG11 liquid culture medium added with 90 mM to 150 mM of ammonium salt, illumination culture is carried out for five days, the growth condition of the OEAsrR9 on the third day is obviously superior to that of WT, and the growth condition of the WT is obviously superior to that of an inhibiting algae strain SPAsrR9. The OEAsrR9 obtained by the invention provides a genetic engineering algal strain chassis for constructing algal strains tolerant to various environmental stresses, provides important theoretical support for high-concentration and high-ammonia wastewater treatment and biological energy production by utilizing microalgae in the future, and has practical significance and wide application prospects.
Resumen de: CN120349880A
The invention provides a multichannel dielectrophoresis microalgae screening device based on a liquid metal bipolar electrode, and relates to the technical field of cell screening. The multichannel dielectrophoresis microalgae screening device based on the liquid metal bipolar electrode comprises a PDMS channel layer and a glass layer which are distributed up and down. According to the invention, cross-channel transmission of a three-dimensional electric field can be realized based on the charge-discharge effect of double electric layers on the surface of the liquid metal three-dimensional bipolar electrode, and cell screening channels have the same electric field intensity distribution and electric field frequency and have the same fluid flow velocity; by means of synchronous regulation and control of an electric field and a flow field, adjustment of the screening effect can be achieved, multiple screening channels are provided, the dielectrophoresis cell screening flux can be remarkably improved, the problem of complex ohmic connection is solved, the risk that cell screening is interfered due to complex ohmic connection is reduced during large-scale multi-channel integration, and the cell screening efficiency is improved. The cell screening flux and screening efficiency are effectively balanced, and meanwhile, the operation cost is reduced.
Resumen de: CN223134435U
The device comprises a culture pond and a heat exchange chamber arranged on one side of the culture pond, a water pump is arranged between the heat exchange chamber and the culture pond, a water inlet of the water pump is connected with the culture pond through a pipeline, and a water storage port of the water pump is connected with the heat exchange chamber through a water conveying pipe. A backflow pipe extending to the position above the culture pond is fixedly connected to the side, opposite to the water conveying pipe, in the heat exchange chamber, a plurality of heat exchange pipes are evenly and fixedly connected to the interior of the bottom side of the heat exchange chamber, and a cleaning assembly used for cleaning the surfaces of the heat exchange pipes is arranged in the heat exchange chamber. The cleaning assembly comprises a floating plate arranged at the bottom of the heat exchange chamber, a cleaning plate arranged at the top of the floating plate and a water inlet pipe fixedly connected to the bottom of the heat exchange chamber. Through the structure, the bristles capable of moving up and down are arranged on the surfaces of the heat exchange pipes, so that the cleanliness of the surfaces of the heat exchange pipes is guaranteed, the heat exchange efficiency of the heat exchange pipes is improved, and normal operation of the microalgae culture water body heat exchange system is guaranteed.
Resumen de: CN223118256U
The utility model discloses domestic sewage treatment equipment for a microalgae bio-membrane reactor, which relates to the technical field of domestic sewage treatment and comprises a treatment cylinder, through holes are circumferentially arrayed on the surface of an upper mounting rack, the mounting rack is arranged between a limiting hole and the through holes, and rotating plates are arrayed on the surface of microalgae bio-membrane filler at equal intervals. When the microalgae biological membrane filler needs to be replaced, the access cover is opened, the upper mounting frame is taken out upwards from the surface of the rotating shaft, then the mounting frame corresponding to the microalgae biological membrane filler needing to be replaced is pulled out from the limiting hole, a new mounting frame is placed into the limiting hole again, and the upper mounting frame is pressed into the surface of the rotating shaft again, so that replacement can be completed. The microalgae biological membrane filler can be independently replaced and maintained, so that the cost is reduced. The rotating shaft is driven to rotate by the speed reducing motor, so that the microalgae biological membrane filler rotates in the treatment barrel and is in full contact with water in the treatment barrel, and the filtering effect is improved.
Resumen de: CN223118462U
The utility model relates to the technical field of cyanobacteria cell wall breaking, and discloses a cyanobacteria cell wall breaking device which comprises a bottom plate, a wall breaking bin and an upper cover assembly, the wall breaking bin is placed at the top of the bottom plate, and electric telescopic rods are installed at the four corners of the top of the bottom plate. The movable end of the electric telescopic rod is fixedly connected with a height extending column, and the upper cover assembly is fixed among the top ends of the four height extending columns. According to the equipment for breaking the wall of the cyanobacteria cells, the range of materials falling into the wall breaking bin is expanded through the arrangement of the multiple discharging holes, the materials are prevented from falling into a certain position in the wall breaking bin in a concentrated mode, meanwhile, the linear motor drives the transverse connecting box to reciprocate so as to change the positions of the discharging holes, and then the materials comprehensively fall into the wall breaking bin; meanwhile, when the transverse connecting box moves, the blue-green algae can be stirred through a stirring plate, the materials are promoted to make full contact with the added raw materials, and the wall breaking effect is improved.
Resumen de: CN223118434U
The utility model relates to the technical field of microalgae culture, in particular to a microalgae culture system based on fishpond culture tail water. According to the technical scheme, the device comprises a water diversion assembly, the water diversion assembly comprises lever frames, a water blocking plate and a waste water pool baffle, the front side and the rear side of a partition baffle are slidably connected with the lever frames, the other end of each lever frame is slidably connected with the water blocking plate, and the waste water pool baffle is fixedly installed on the inner wall of the runway type culture pond. And the water blocking plate slides up and down on the side part of the wastewater pool baffle. In summer, water in the fishpond is exposed to the sun, the water level of wastewater for culturing microalgae is lowered, the separation baffle moves downwards along with the water at the moment, the separation baffle enables the water blocking plate to move upwards through the lever frame, and the water level of the left side of the water blocking plate is higher than that of the right side of the water blocking plate, so that the water on the left side of the water blocking plate is introduced to the right side, and the water for culturing microalgae is supplemented in time; the microalgae are prevented from being washed away by water due to the fact that the height of the microalgae is lower than the partition baffle.
Resumen de: CN223118253U
The utility model relates to the technical field of wastewater purification, in particular to a fishpond breeding wastewater purification device. According to the technical scheme, the device comprises a flow guide part, the flow guide part comprises a baffle, a flow guide exchange plate and a water row, the baffle is fixedly installed in a culture pond and located on the water surface of ammonia nitrogen wastewater, and the flow guide exchange plate is fixedly installed in the culture pond and located below the baffle; and a water drain is rotationally connected to the interior of the culture pond and located at the extension line of the flow guide exchange plate. The microalgae absorb ammonia nitrogen in the ammonia nitrogen wastewater, the microalgae and plankton are used as live bait of fish, the cost is reduced, the water quality is purified, the ammonia nitrogen wastewater is drained through the flow guide exchange plate, the ammonia nitrogen wastewater located on the lower layer flows to the upper layer, the microalgae absorb the ammonia nitrogen in the ammonia nitrogen wastewater, and the water quality is improved. The ammonia-nitrogen wastewater guided by the guide exchange plate impacts the water row, so that the water row rotates, and the ammonia-nitrogen wastewater at the upper layer and the lower layer is further mixed.
Resumen de: CN120328725A
The invention discloses a Z-shaped sewage removal device for microalgae co-culture, and belongs to the technical field of sewage treatment devices. According to the method, the characteristic that scenedesmus and chlorella are co-cultured to synergistically promote nutrition utilization and algae growth under a severe environment condition is utilized, carbon dioxide in sewage is absorbed through photosynthesis under an illumination condition and is converted into organic substances, and nutrient substances such as ammonia nitrogen in the sewage are utilized for growth; furthermore, by constructing a non-immersed Z-shaped reactor, light can directly irradiate the microalgae attached to the surface of the cotton cloth, light attenuation caused by high turbidity of organic sewage in a farm is overcome, meanwhile, the landing area of the microalgae is increased, the removal capacity of a microalgae biological membrane is further improved, finally, ammonia nitrogen in the sewage is removed, and the sewage treatment efficiency is improved. And the microalgae biomass with economic value is also produced.
Resumen de: CN120328739A
The invention discloses a sewage treatment method of a polymorphic nitrogen synergistic metabolism removal system based on a microalgae encapsulation technology, and relates to a sewage treatment method of a polymorphic nitrogen synergistic metabolism removal system. The invention aims to solve the problems that the traditional microalgae sewage treatment technology is difficult to realize efficient synergistic metabolism of nitrogen elements in various forms, so that the treatment effect is unstable, and the treatment requirements of wastewater with different water qualities are difficult to meet. According to the invention, specific algae species are adopted, microalgae cells are fixed in gel microspheres through a packaging process, and a micro-processing system in which an outer high-light-transmission area algae group executes light capture and an inner low-light area algae group is responsible for energy conversion is formed. According to the system, smooth transmission of nitrogen is ensured, the demand of microalgae on nitrogen is met, nitrogen metabolism based on differential photosynthetic tasks among layers is constructed and regulated based on a dissolved oxygen/illumination gradient synergistic microenvironment, and efficient synergistic metabolism removal of polymorphic nitrogen is realized. The invention belongs to the technical field of sewage treatment.
Resumen de: CN120330116A
The invention discloses a construction method of a cyanobacteria-corynebacterium glutamicum artificial photosynthetic mixed bacteria system. The construction method comprises the following steps: step S1, amplifying by taking plasmid pUC-tho as a template to obtain a theophylline inducible promoter Ptho; step S2, carrying out amplification by taking a Synechocystis sp. PCC 6803 genome as a template so as to obtain a strong promoter Pcpc560, and carrying out amplification on the strong promoter Pcpc560 so as to obtain a strong promoter Pcpc560; step S3, carrying out amplification by taking the plasmid pUC-PAL as a template to obtain an arabidopsis thaliana phenylalanine lyase gene pal; step S4, carrying out amplification by taking the plasmid pUC-cscB as a template to obtain an escherichia coli W sucrose transporter gene cscB; step S5, carrying out amplification by taking the integrated plasmid Pcp3031 as a template, so as to obtain a terminator Trbcl; and step S6, fusing the strong promoter Pcpc560, the arabidopsis phenylalanine lyase gene pal and the terminator Trbcl, and respectively connecting with an expression vector pSI-SPE to obtain the expression vector containing the gene pal. According to the invention, a'cyanobacteria-corynebacterium glutamicum 'artificial photosynthetic mixed bacteria system is constructed by simulating a mutualistic symbiotic relationship ubiquitous in nature, and negative carbon biosynthesis of cis-muconic acid is realized.
Resumen de: CN120323370A
The invention belongs to the field of aquaculture engineering, and particularly relates to an automatic identification and feeding shellfish larva cultivation system and method, and the system comprises a larva circulating water cultivation tank which is used for accommodating shellfish larvae and a circulating water body; the larva image acquisition device is used for acquiring larva image data and sending the larva image data to the control system; the peristaltic pump is used for receiving the control of the control system and quantitatively adding the bait algae liquid; the algae density sensor is used for collecting real-time microalgae concentration; the control system is used for receiving the image data of the larva image acquisition device and analyzing and acquiring the number of larvae and the shell length of the larvae; data of the algae density sensor is also received and converted into real-time microalgae concentration; meanwhile, according to the obtained data, the amount of bait algae liquid needing to be supplemented is calculated, the peristaltic pump is controlled to be started, and feeding is executed. The accurate feeding control system for the shellfish larvae is innovatively researched and developed, and the larva feeding amount is accurately controlled on the basis of the constructed accurate feeding preset model.
Resumen de: CN120330055A
The invention provides a recombinant algal strain for expressing AGAT, BHAA, BHAD and ISR as well as a construction method and application of the recombinant algal strain, and belongs to the technical field of gene engineering. The invention provides a recombinant microalgae strain, which is a microalgae strain for expressing aspartic acid-glyoxylate transaminase, beta-hydroxyaspartic acid aldolase, beta-hydroxyaspartic acid dehydratase and iminosuccinic acid reductase. The invention further provides a preparation method of the recombinant microalgae strain. The recombinant microalgae strain can decompose a light respiration by-product glycollic acid into carbon dioxide, the carbon dioxide concentration of chloroplast is improved, the carbon sequestration efficiency of microalgae is effectively improved, and fixed CO2 can be converted into biomass, pyruvic acid, starch, grease and other metabolites and bioactive substances. The recombinant microalgae strain disclosed by the invention has a wide application prospect in the aspects of biological carbon sequestration, synthesis of microalgae metabolites and preparation of microalgae bioactive substances.
Resumen de: CN120330036A
The invention discloses a photobioreactor device for microalgae culture, and relates to the technical field of biological growth containers, the photobioreactor device comprises a frame, a mounting rack I, a controller and an incubator shell, a reaction barrel is rotatably arranged in the incubator shell, and a plurality of lantern boxes are sequentially arranged in the reaction barrel from top to bottom; the top of the outer side of each lantern box is provided with a drainage ring, the bottom end of the bottommost lantern box is fixedly provided with a fourth flow guide pipeline, and a plurality of first exhaust pipes used for releasing pressure in the reaction barrel are internally provided with sampling assemblies. Under the conditions that algae cultivation is not affected and waste gas leakage is little, algae samples are obtained from different positions in the photobioreactor device for multiple times, and the culture effect of the photobioreactor device can be conveniently detected.
Resumen de: CN120325124A
The invention relates to the technical field of biogas slurry treatment, and provides a device and a method for treating biogas slurry after anaerobic fermentation of organic garbage based on symbiosis of microalgae and bacteria, the device comprises a biogas slurry treatment box, a diversion recycling box, an outer water storage box, a water pump, a rotating shaft, stirring blades and a driving mechanism, the biogas slurry treatment box and the diversion recycling box are both used for storing biogas slurry; the outer water storage tank is used for storing dilution water, the water pump is arranged on the biogas slurry treatment tank, the outer water storage tank is communicated with a water pumping pipe, the water inlet end of the water pump is communicated with a connecting pipe communicated with the water pumping pipe, and the water outlet end of the water pump is communicated with a water supply pipe communicated to the interior of the biogas slurry treatment tank. And the water supply pipe extends to the top end of the inner cavity of the biogas slurry treatment box. According to the biogas slurry treatment device, when the nutrient content proportion in the biogas slurry is relatively high, the biogas slurry in the biogas slurry treatment box can be diluted in time, so that the treatment efficiency of the biogas slurry in the biogas slurry treatment box is improved, and the biogas slurry treatment device is convenient to use.
Resumen de: WO2025148196A1
Provided is use of a microalgae extract for promoting the absorption and permeation through the blood-brain barrier of a fatty acid and a composition thereof. The microalgae extract comprises a polar lipid in a proportion of 30% to 99.9%. The fatty acid is a fatty acid contained in a non-microalgae extract. The provided use of the microalgae extract for promoting the absorption and permeation through the blood-brain barrier of a fatty acid and the composition thereof can, by means of the cooperation of the microalgae extract and the fatty acid, promote the absorption of the fatty acid in a user, and particularly, increase the content of the fatty acid in the heart and the brain.
Resumen de: WO2025149866A1
The present invention relates to the use of a new strain of the microalgal species Chlorella vulgaris for producing lutein and zeaxanthin, as well as to the method for obtaining it.
Resumen de: US2025230388A1
A system and method for quantifying CO2 capture by photobioreactors using microalgal biomass are disclosed. The method involves accurately quantifying carbon dioxide capture by photobioreactors using microalgal biomasses involves determining a unique ratio relating carbon consumed to biomass produced for each microalgae strain. This ratio is then used to predict CO2 removal from flue gases or industrial processes based on the dry mass of the corresponding biomass produced. Machine learning models are also trained on data obtained from the photobioreactors to predict CO2 consumption rates under varying conditions, thereby enhancing the accuracy and efficiency of carbon capture technologies.
Resumen de: CN120309092A
The invention discloses a sewage treatment method and system based on simulation analysis, and the method comprises the steps: firstly, dividing a polluted water body region into K sub-regions, carrying out the microalgae putting and pollution monitoring of each sub-region based on a preset microalgae regulation and control scheme in a measurement time period, and collecting measurement data and pollution condition data; migration coefficients, growth coefficients and pollution change rates of the sub-regions are calculated by periodically analyzing migration, growth and pollution changes of the microalgae. Performing linear fitting by taking the migration coefficient and the growth coefficient as independent variables and the pollution change rate as a dependent variable, and searching an optimal fitting parameter through a gradient descent optimization algorithm to obtain a fitting equation of each sub-region; further, the sub-regions are grouped based on migration and growth characteristics. And searching local and global optimal solutions by taking pollution change rate minimization as a target through a Bayesian optimization algorithm, and setting an optimal microalgae regulation and control scheme for each region group in combination with microalgae resources so as to realize efficient treatment of polluted water.
Resumen de: CN120310653A
The invention discloses a flocculation-controllable genetic engineering alga and a construction method and application thereof, and is characterized in that the genetic engineering alga is phaeodactylum tricornutum into which an enabling plasmid pPha-J47869-EG01992-eGFP-FSP is introduced, the FSP is directionally expressed on the outer side of a phaeodactylum tricornutum cell, and the construction method comprises the following steps: constructing an induced expression plasmid pPha-J47869-eGFP and a directional expression plasmid pPha-J47869-EG01992-eGFP; artificially synthesizing a codon-optimized FSP coding sequence, inserting the codon-optimized FSP coding sequence into the downstream of the eGFP of the directional expression vector, and constructing an enabling plasmid; finally, the enabling plasmids are electrically transformed into phaeodactylum tricornutum, an f/2 plate culture medium is coated with the phaeodactylum tricornutum for culture, positive algae strains are screened and identified, and the gene engineering algae with controllable flocculation are constructed and have the advantages that the gene engineering algae can be efficiently combined with white carbon black, and the enabling process of microalgae is efficiently coupled with the culture and collection process of the microalgae.
Resumen de: KR20250107436A
본 발명은 해양미세조류 듀나리엘라 살리나 (Dunaliella salina DSTA20) 유래 엑소좀의 피부 미백 및 항염증 효능에 관한 것으로, 해양 미세조류인 듀나리엘라 살리나 미세조류 배양을 통한 엑소좀이 멜라닌 생성을 저해하고 타이로시네이즈(Tyrosinase) 효소를 저해시킴을 확인하고, 일산화질소(NO)를 저해시키는 것을 확인하여 피부미백 또는 항염용 조성물, 화장료 조성물 또는 건강기능식품 조성물 등 다양하게 활용할 수 있다.
Resumen de: CN120290324A
The invention belongs to the technical field of biological sewage treatment, and relates to a bacteria-algae particle preparation method and system for promoting bacteria-algae system granulation based on pressure response. The preparation method comprises the following steps: co-culturing the mixed microalgae cultured to a logarithmic phase and aerobic activated sludge in a photobioreactor, in the culture process, jointly regulating and controlling aeration, a carbon source and inlet and outlet water to promote granulation of a bacteria-algae system according to the principle of'pressure-induced stress response ', and controlling dissolved oxygen levels in different periods according to the principle of'unbalanced air supply' during aeration regulation and control; the carbon source regulation follows the principle of unbalanced nutrition to control the COD concentration in different time periods; the water inlet and outlet regulation follows the principle of reducing the settling time, and the water change settling time is gradually shortened in the culture period. The bacteria-algae granular sludge which is good in settling performance, high in dirt-removing power and compact in structure is prepared, the prepared bacteria-algae granular sludge is used for treating sewage and wastewater, pollution of carbon, nitrogen, phosphorus and the like can be efficiently solved, the effluent quality is effectively improved, and carbon emission reduction in the sewage treatment field
Resumen de: CN120290329A
The invention provides a recombinant algal strain for expressing glycollic acid oxidase, catalase and oxalate oxidase as well as a construction method and application of the recombinant algal strain, and belongs to the technical field of genetic engineering. The invention provides a recombinant microalgae strain, which is a microalgae strain for expressing glycollic acid oxidase, catalase and oxalate oxidase in chloroplast. The recombinant microalgae strain can decompose a light respiration by-product glycollic acid into carbon dioxide, the carbon dioxide concentration of chloroplast is improved, the carbon sequestration efficiency of microalgae is effectively improved, and fixed CO2 can be converted into biomass, glucose, cane sugar, starch, grease and other metabolites and bioactive substances. The recombinant microalgae strain disclosed by the invention has a wide application prospect in the aspects of biological carbon sequestration, synthesis of microalgae metabolites and preparation of microalgae bioactive substances.
Nº publicación: CN120290326A 11/07/2025
Solicitante:
JIANGSU RISHENGCHANG BIOLOGICAL TECH CO LTD
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Resumen de: CN120290326A
The invention relates to a microalgae culture medium based on plant extracts and a preparation method thereof, and belongs to the technical field of algae culture media, the microalgae culture medium specifically comprises the following raw materials: blueberry peel extracts, cellulose nanofibers, a carbon source, a system stabilizer, inorganic salt, trace elements and sterile seawater; the carbon source is obtained by mixing glycerol and pretreated cane molasses according to a mass ratio of 1: (2-2.5). In the preparation method, processes in different components are coupled, the cellulose nanocrystals are extracted in an acid environment, and meanwhile, the extraction amount of the cellulose nanocrystals and the activity of anthocyanin and the like are regulated and controlled through the pH value, so that the characteristics of stability, oxidation resistance and the like of the components in the extraction process are maintained. The cane molasses is pretreated, and then the cane molasses and glycerol are jointly used as a carbon source, and the cane molasses and the blueberry peel extract have a synergistic effect in the aspect of promoting microalgae growth.