Alerta

Method for purifying wastewater by using MgFe-LDH modified charcoal reinforced microalgae

Resumen de: CN121225767A

The invention relates to a wastewater purification method, in particular to a method for purifying wastewater by using MgFe-LDH modified biochar reinforced microalgae, which comprises the following steps: preparing MgFe-LDH modified biochar; co-culturing the modified biochar and microalgae, and determining the optimal addition amount of the MgFe-LDH modified biochar by comparing the growth condition of the microalgae; and inoculating the MgFe-LDH modified charcoal and microalgae into the wastewater for purification. Compared with the prior art, the method has the advantages that a CO2-rich microenvironment is created for the microalgae in the wastewater by constructing an algae-carbon co-culture system, the inherent limitation of insufficient carbon supply in a solution is improved, the growth of the microalgae in the wastewater is promoted, and the wastewater purification effect of the chlorella is further enhanced. The method is simple and convenient to operate, low in preparation cost, high in wastewater purification efficiency of microalgae and high in environment adaptability of an algae carbon system, and the purposes of fixing CO2 and efficiently purifying wastewater can be achieved.

Energy-saving illumination and temperature coordinated regulation and control device

Resumen de: CN223738047U

The utility model discloses an energy-saving illumination and temperature coordinated regulation and control device, and belongs to the field of microalgae cultivation. An energy-saving illumination and temperature coordinated regulation and control device comprises a supporting plate and further comprises a light guide pipe fixedly connected to the supporting plate, a diffuser is arranged at the bottom of the light guide pipe and arranged below the supporting plate, and a brightness sensing assembly is arranged in the light guide pipe; the connecting plate is fixedly connected to the bottom of the supporting plate, an illuminating lamp is connected to the connecting plate in a lifting mode, a temperature sensing assembly is arranged on the illuminating lamp, and when the light source in the light guide pipe is detected by the brightness sensing assembly, the temperature sensing assembly and the illuminating lamp ascend or descend; according to the utility model, the position height and the switch of the illuminating lamp can be controlled, and the illuminating lamp is matched with the light guide pipe for use, so that unnecessary power consumption can be reduced, and the energy efficiency is improved.

Ecological intensive mariculture tail water intelligent treatment system and method

Resumen de: CN121225799A

The invention belongs to the technical field of environmental engineering and pollution treatment, and particularly relates to an ecological intensive mariculture tail water intelligent treatment system and method.The ecological intensive mariculture tail water intelligent treatment system comprises a pretreatment and solid waste recycling module, a primary purification module, a biological collaborative purification module and a deep purification module; the pretreatment and solid waste recycling module comprises a high-density sedimentation tank, the primary purification module comprises a microalgae open pond, the biological collaborative purification module comprises a mixed culture area, and the deep purification module comprises an artificial wetland area. An outlet in the upper end of the microalgae open pond is connected with an inlet in the upper end of the mixed culture area through a pipeline I; according to the system, a biological treatment mode is adopted, the inherent defect of a single method in the prior art is overcome, standard discharge of mariculture tail water is achieved, green, ecological and efficient development of the breeding industry is promoted, and the win-win situation of ecological environment protection and economic benefit improvement is achieved.

System and process for preparing biogas through microalgae carbon sequestration and biogas purification

Resumen de: CN121227405A

The invention discloses a system and a process for preparing biogas through microalgae carbon sequestration and biogas purification. The system comprises a photosynthetic carbon sequestration biogas purification reactor module, a gas detection system, a deoxygenation module and a microalgae biomass collection module, the photosynthetic carbon sequestration biogas purification reactor module is a multi-stage closed photosynthetic bioreactor, and all stages of closed photosynthetic bioreactors are connected in sequence; marsh gas enters from a gas inlet of the first-stage closed photosynthetic bioreactor and sequentially passes through all stages of closed photosynthetic bioreactors for marsh gas purification, and gas at a gas outlet of the last-stage closed photosynthetic bioreactor enters a deoxidizing device for deoxidizing; and the gas which does not reach the purification standard of each stage of reactor flows back to the current stage of reactor for re-purification. The process provided by the invention is used for treating biogas generated by anaerobic digestion of organic wastes, introducing the biogas into a bioreactor, and absorbing CO2 by microalgae to realize gas purification.

Ultrasound-controllable multifunctional microneedle patch as well as preparation method and application thereof

Resumen de: CN121197013A

The invention discloses an ultrasonic controllable multifunctional microneedle patch as well as a preparation method and application thereof, and belongs to the technical field of biological materials. The preparation method comprises the following steps: preparing crystalline CoW-LDH nanosheets from Co (NO3) 2.6 H2O, Na2WO4. 2H2O and NaOH, dispersing the crystalline CoW-LDH nanosheets in PBS (Phosphate Buffer Solution), centrifuging and collecting the crystalline CoW-LDH nanosheets, and re-dispersing the crystalline CoW-LDH nanosheets in ultrapure water to obtain amorphous CoW-LDH nanosheets; adding PEG (Polyethylene Glycol), ultrasonically dispersing, stirring at room temperature, centrifuging, collecting and centrifugally washing with ultrapure water to obtain a PEG modified amorphous CoW-LDH nanosheet; and adding microalgae and an amorphous CoW-LDH nanosheet modified by PEG (Polyethylene Glycol) to prepare the ultrasonic controllable multifunctional microneedle patch. The multifunctional microneedle patch disclosed by the invention is high in biological safety, can realize ultrasonic controllability, has a sonodynamic treatment effect and an oxygen supply effect, remarkably inhibits melanoma recurrence and remarkably promotes wound healing, and has a wide application prospect.

Fucoxanthin synthesis optimization method and system based on reinforcement learning

Resumen de: CN121215064A

The invention relates to the technical field of biological environment engineering, and provides a fucoxanthin synthesis optimization method and system based on reinforcement learning, and the method comprises the following steps: a real-time data acquisition system; constructing a state space; a deep reinforcement learning decision network; optimizing a dynamic strategy; and strategy verification is linked with the process. Through a light quality-stimulant collaborative reinforcement learning regulation strategy, the static operation limitation of a traditional process is broken through, the photosynthetic physiological state of microalgae is perceived in real time, the light environment and chemical signals are dynamically optimized, the fucoxanthin synthesis efficiency is remarkably improved, and a multi-omics closed-loop verification mechanism is combined, so that the fucoxanthin synthesis efficiency is improved. The molecular cascade effect of a light signal driving pigment synthesis pathway is illuminated, and a precise target spot is provided for metabolic engineering transformation.

Blue-green algae collecting device based on magnetic carrier and collecting method of blue-green algae collecting device

Resumen de: CN121203807A

The invention discloses a blue-green algae collecting device based on a magnetic carrier and a collecting method of the blue-green algae collecting device, and belongs to the technical field of blue-green algae treatment. The device comprises a liquid storage unit, a magnetic separation unit and a collection unit, the magnetic separation unit is matched with the liquid storage unit, the magnetic separation unit generates a directional magnetic field and acts on mixed liquid in the liquid storage unit, so that magnetic carriers combined with blue-green algae in the mixed liquid are gathered in the acting direction of magnetic field force, and the blue-green algae-magnetic carrier compound is formed. The magnetic carrier is prepared, and polydopamine (PDA) is rich in active groups, so that the affinity and binding force to cyanobacteria cells are enhanced; meanwhile, a buffer solution is used for accurately regulating and controlling pH, the optimal environment for carrier synthesis and blue-green algae combination is guaranteed, and compared with an unmodified carrier, the blue-green algae capture rate is remarkably increased.

Concrete-microalgae co-production system for carbon dioxide curing

Resumen de: CN121203785A

The invention belongs to the technical field of waste utilization devices, and particularly relates to a concrete-microalgae co-production system for carbon dioxide curing. The co-production system comprises a carbon dioxide trapping device, a microalgae culture device and calcining equipment; the microalgae culture device comprises a culture box, the bottom of the culture box is provided with an air inlet, a slag discharge port and an oil discharge port, and the top of the culture box is provided with an air outlet; a gas outlet of the carbon dioxide trapping device is communicated with a gas inlet of the incubator, and a slag discharging opening of the incubator is communicated with a feeding opening of the calcining equipment. According to the co-production system, the carbon dioxide trapping device, the microalgae culture device and the calcining equipment are connected for use, industrial waste gas is treated by the carbon dioxide trapping device, then carbon dioxide is introduced into the microalgae culture device, the carbon dioxide is efficiently fixed through photosynthesis of microalgae, and algae oil (biofuel) and algae residues are synchronously produced; and the algae residues are reused for cement clinker firing, so that a closed-loop industrial chain of carbon capture-biomass energy-building material regeneration is realized.

Method for improving orientated conversion of perigord truffle fatty acid into EPA and application of method

Resumen de: CN121204175A

The invention relates to the technical field of bioengineering and food engineering, in particular to a method for improving directional conversion of perigord truffle fatty acid into EPA and application of the perigord truffle fatty acid into the EPA. According to the system, a natural enzyme system and active ingredients in a nannochloropsis oculata lysate are fully utilized as biological catalytic components, and the physical strengthening effect of multi-frequency ultrasound is combined; under mild conditions, the unsaturated efficiency of fatty acid and the generation rate of EPA in a reaction system are remarkably improved. According to the method, perigord truffle is subjected to ethanol extraction, lipid components of perigord truffle are fully released, and the perigord truffle and a microalgae lysate have a synergistic effect, so that a composite reaction system with biological catalysis and physical promotion functions is constructed. The method is simple and controllable in process, has the advantages of being short in reaction time, low in energy consumption, environmentally friendly and the like, and meanwhile achieves the dual purposes of high-value utilization of perigord truffle resources and sustainable production of EPA. According to the method, an innovative solution is provided for high-value utilization of perigord truffle resources.

Composite material and method for accelerating diatom sedimentation

Resumen de: CN121203818A

The invention relates to the field of microalgae sedimentation, in particular to a composite material for accelerating diatom sedimentation and a method thereof. The composite material comprises chitosan and clay, wherein the mass ratio of the chitosan to the clay is (1: 5)-(1: 15). The sedimentation method comprises the following steps: adding the composite material into diatom liquid, culturing for a certain time, and collecting the diatom. By implementing the scheme, the recovery rate of the diatom is improved.

Accumulation method of cyanobacteria grease and high value-added metabolites under nitrogen starvation stress

Resumen de: CN121204176A

The invention relates to the technical field of preparation of blue-green algae biological resources, in particular to a method for accumulating blue-green algae grease and high-added-value metabolites under nitrogen starvation stress, which comprises the following steps: after carrying out plasma mutagenesis screening on blue-green algae, inoculating an improved BG-11 culture medium and activating to OD680 1.2-1.8; carrying out gradient switching of a nitrogen source according to an inoculum size of 10-15%, carrying out pre-culture for 4-6 days, and regularly supplementing vitamin C; carrying out flow monitoring on the nitrogen content of the cells, and carrying out mild and severe dynamic nitrogen starvation stress culture for 4-7 days; adding a nano-loaded composite inducer on the first day of severe stress, and performing pulse stirring culture for 2-3 days; after near-infrared monitoring reaches the standard, antioxidant is added for harvesting, and the biomass is subjected to freezing pretreatment. The high value-added metabolite is an ultraviolet absorbing substance (mycosporine-like amino acid and pseudocystin), phycocyanin or gamma-linolenic acid. According to the method, high-yield strains are screened through mutagenesis, gradient pre-culture and dynamic nitrogen starvation regulation are combined, and a nano-loaded composite inducer and real-time monitoring are matched, so that the grease and metabolite accumulation efficiency is directionally improved, and the ca

Method for promoting microalgae growth by using pharmaceutical product

Resumen de: CN121203932A

The invention belongs to the technical field of microalgae biology and culture, and particularly relates to a method for promoting microalgae growth by using a pharmaceutical product, which comprises the following steps: activating microalgae on a solid flat plate, inoculating the activated microalgae into a liquid culture medium, adding the pharmaceutical product at the initial stage of microalgae growth, placing in a constant-temperature shaking table, and carrying out illumination culture. According to the method, different types of pharmaceutical products with different concentrations are added into the microalgae, the influence of the addition of the different types of pharmaceutical products with different concentrations on the growth promotion of the microalgae is analyzed, the biomass of the chlorella can be rapidly increased, and the method has important significance on the large-scale production of the microalgae.

Novel sewage ecological treatment system and treatment method

Resumen de: CN121202360A

The invention discloses a novel sewage ecological treatment system and a treatment method. Comprising a biological membrane filter and a multi-medium ecological filter bed, wherein the biological membrane filter sequentially comprises a ventilation tower, a water inlet system, a biological contact oxidation zone, an air inlet and a water storage tank from top to bottom, and the air inlet and the parts above the air inlet are arranged above the ground; the multi-medium ecological filter bed is arranged corresponding to the water storage tank; the multi-medium ecological filter bed sequentially comprises a biological contact denitrification area, a sand filtration area, a special functional area and a microalgae and plant purification area from bottom to top; and the lower part of the water storage tank of the biological membrane filter distributes water to the biological contact denitrification area of the multi-medium ecological filter bed through a secondary water distribution pipe. The system disclosed by the invention can be used as a tail water advanced treatment facility of municipal and industrial sewage plants, and has the advantages of low energy consumption, low maintenance and operation cost, high sewage treatment efficiency, strong adaptive capacity and impact resistance and difficulty in blockage.

ENHANCED TRIACYLGLYCEROL PRODUCTIVITY AND EXTRACTABILITY IN AN ENGINEERED MICROALGA

Resumen de: US2025388850A1

The present invention relates to an engineered unicellular Stramenopile microalga comprising a loss of function of the homologous Seipin gene, an in vitro method of producing triacylglycerols (TAG), and uses thereof.

ALGAE CULTIVATION AND HARVESTING FROM CARRIERS

Resumen de: US2025388844A1

Microalgae are grown in a biofilm on carriers. The carriers may have surfaces with a small angle to the vertical, optionally forming a cone. A surface of the carrier may be flexible and optionally include a porous material. Algae are harvested from the carriers using a water jet, which may provide a substantially solid stream of water. A nozzle for producing the water jet may spin while being moved laterally over a carrier bed. The water jet may dislodge algae from a surface of the carriers in the form of large agglomerates or blobs. The dislodged algae may have a high concentration of solids or readily settle. The algae may be collected from a floor below the carriers, optionally with a moving collection device. A moving harvesting device may treat a portion of a carrier bed such that harvesting of the entire bed is distributed over time.

ANIMAL PROTEINS IN CYANOBACTERIA

Resumen de: WO2024170959A2

In some embodiments, the gene is integrated into the genome of the cyanobacteria. In some of these embodiments, the gene is integrated into an NS1 site of the cell. The sequence encoding an animal protein is optimized for expression in the cyanobacteria. In some embodiments, the animal protein is a muscle protein. In some of these embodiments, the muscle protein is a myosin subunit, myoglobin, or alpha-actin. In other embodiments, the animal protein is a subunit of a complex. In some of these embodiments, the complex is a myosin complex. The production of proteins by modified cells may be performed in multiple different cells, in some embodiments, the cyanobacteria cell is S. elongatus is PCC7942 or UTEX2973 or PCC7002, or Synechocystis sp. PCC6803. In some embodiments, multiple genes are incorporated into a cell. In some embodiments, each gene comprises a myosin complex subunit. The myosin complex subunits are MLC1, MLC2, and MYH2.

Fishery breeding tail water treatment and nitrogen and phosphorus resource utilization algae culture device

Resumen de: CN223705378U

The utility model belongs to the technical field of fishpond breeding tail water treatment, and particularly relates to a fishpond breeding tail water treatment and nitrogen and phosphorus resource utilization algae culture device. The algae culture device for fishery breeding tail water treatment and nitrogen and phosphorus resource utilization comprises a front-end biological treatment unit, a nitrogen and phosphorus resource utilization algae culture unit, an effluent oxygenation unit and a device chamber, the front-end biological treatment unit comprises a first-echelon biological treatment device and a second-echelon biological treatment device, the first-echelon biological treatment device, the second-echelon biological treatment device and the nitrogen and phosphorus resource utilization algae culture unit are sequentially connected, and the nitrogen and phosphorus resource utilization algae culture unit is provided with an LED lighting system and a stirring device. The effluent oxygenation unit is connected with the nitrogen and phosphorus resource utilization algae culture unit and is provided with a stepped oxygenation device. According to the device, the carbon, nitrogen and phosphorus treatment of the fishpond culture tail water is realized, and the effluent rich in microalgae flows back into the fishpond, so that the culture tail water is recycled, and the feed feeding is reduced. In addition, the equipment also has the advantages of high integration level, small

Open shallow water microalgae adherent culture device

Resumen de: CN223705560U

The utility model relates to the technical field of algae culture devices, in particular to an open shallow-water microalgae adherent culture device which comprises a transverse rectangular supporting frame, a plurality of culture substrates are sequentially and flatly laid on the supporting frame from top to bottom at equal intervals, and the section of each culture substrate is in a groove shape. One end of the culture substrate is provided with an integrated blocking plate, and the other end is open and hermetically connected with a funnel; the funnel is embedded into one side of the blocking plate of the adjacent culture substrate; a plurality of incandescent lamps are arranged between every two adjacent culture substrates in the transverse direction at equal intervals, and the incandescent lamps are fixedly arranged on the supporting frame in the longitudinal direction. The diatom growth is promoted through natural light and an incandescent lamp, and a thin culture solution is formed on the bottom surface of the culture substrate, so that the diatom can statically grow on the bottom surface of the culture substrate and finally form a biological membrane with a certain thickness, and the biological membrane can be directly observed and collected.

Method for verifying function of Cre in oil-producing microalgae and application of Cre in enhancing biosynthesis of eicosapentaenoic acid

Resumen de: CN121160751A

The invention provides a method for verifying functions of Cre in oil-producing microalgae and application of the method in enhancing biosynthesis of eicosapentaenoic acid, and belongs to the technical field of genetic modification. According to the invention, functional verification of Cre in oil-producing microalgae is completed by a method of inserting an intron from an endogenous Phatr3J51169 gene of phaeodactylum tricornutum into a Cre gene, that is, a selection marker located between two loxP sequences can be removed, and a free plasmid transformation method more suitable for Cre removal is further developed. Based on a Cre/loxP system, the expression of EPA synthetic pathway genes in the oil-producing microalgae is enhanced to obtain an engineering algal strain with high yield of EPA, and compared with a wild type, the relative content of EPA can be increased by 24.7%.

Light-wind-heat coupling enhanced hot spring thermophilic cyanobacteria culture photobioreactor

Resumen de: CN121160439A

The invention discloses a light-wind-heat coupling enhanced hot spring cyanobacteria culture photobioreactor. The photobioreactor comprises a reactor main body, a light source system, a floating type supply device, an attachment device, a control system and an energy supply system, wherein the reactor main body is connected with the energy supply system, and energy supply to the reactor main body is completed by utilizing light energy and wind energy; a floating type supply device is arranged at the bottom of the reactor main body and is used for providing sufficient CO2 and other nutrient substances for the thermophilic cyanobacteria; the thermal insulation cylinder is arranged in the reactor main body, so that heat dissipation in the bioreactor is remarkably reduced; an adhesion system is arranged in the reactor, so that the adhesion habitat environment of the thermophilic cyanobacteria in the hot spring is reduced, and later recovery is facilitated. According to the invention, environmental conditions required by growth and reproduction of thermophilic cyanobacteria in the hot spring can be automatically controlled in a low-carbon clean state, and a reliable scheme is provided for ectopic large-scale culture of thermophilic microorganisms.

Chlamydomonas reinhardtii metabolic enzyme protein nuclear localization method based on BCCP fusion targeting

Resumen de: CN121160750A

The invention discloses a BCCP fusion targeting-based chlamydomonas reinhardtii metabolic enzyme protein nuclear localization method, which comprises the following steps: carrying out fusion expression on acetyl coenzyme A carboxylase (ACC) biotin carboxyl carrier protein (BCCP) subunit and target protein, and realizing specific enrichment of fusion protein on the periphery of a protein nucleus by utilizing the natural protein nucleus targeting characteristic of BCCP. The invention provides a novel method for constructing a chlamydomonas reinhardtii engineering strain capable of efficiently utilizing inorganic carbon; the method has important application value in the fields of microalgae carbon capture, biofuel production, high-added-value compound synthesis and the like.

Aluminum electrolysis flue gas treatment and recovery method based on microalgae staged culture

Resumen de: CN121155338A

The invention provides an aluminum electrolysis flue gas treatment and recovery method based on microalgae staged culture, and belongs to the field of biological purification and recycling of industrial flue gas. The method comprises the following steps: pretreating the aluminum electrolysis flue gas to obtain purified flue gas; introducing the purified flue gas into a photobioreactor inoculated with fluorine-resistant microalgae, and carrying out I-stage tolerance period culture to obtain an algae solution; transferring the algae liquid into a photobioreactor, adding CaCl2 into the algae liquid and limiting the supply of a nitrogen source, and introducing purified flue gas into a second photobioreactor to carry out II-stage accumulation period culture, so as to obtain algae slurry; carrying out solid-liquid separation on the algae slurry to obtain algae mud and algae-containing filtrate; the algae mud is subjected to wall breaking treatment and supercritical extraction, and algae oil and algae residues are obtained. According to the application, the pollutant tolerance mechanism and the product synthesis path of the microalgae are triggered by regulating and controlling environmental factors, so that efficient purification of flue gas, fixation of fluorine in the form of biominerals, step-by-step extraction of high-value products from algae and cyclic utilization of algae residues can be synchronously realized.

Long-term operation and resourceful treatment method of phycomycete

Resumen de: CN121158966A

The invention belongs to the technical field of phycomycete sewage recycling, and particularly relates to a phycomycete symbiotic system long-term operation and resourceful treatment method which comprises the following steps: constructing a phycomycete symbiotic system containing microalgae and activated sludge; the phycomycete symbiotic system is placed in an optical sequencing batch reactor to operate, the chitosan-diatomite composite material is added in the operation process, and the chitosan-diatomite composite material is added after each time of drainage; the method comprises the following steps: preparing a phycomycete suspension, carrying out vacuum freeze drying on the phycomycete suspension to obtain freeze-dried phycomycete mud, grinding the phycomycete mud into powder, and extracting lipids in the powder. The method provided by the invention can stably operate for a long time, not only improves the removal effect of pollutants, but also remarkably enhances the flocculation performance and lipid productivity of a phycomycete system, has the characteristics of pollutant removal and efficient recycling of resource products, and has wide application prospects of sewage resource utilization and biological energy development.

Method for promoting microalgae growth by using pharmaceutical product

Resumen de: CN121160592A

The invention belongs to the technical field of microalgae biology and culture, and particularly relates to a method for promoting microalgae growth by using a pharmaceutical product, which comprises the following steps: activating microalgae on a solid flat plate, inoculating the activated microalgae into a liquid culture medium, adding the pharmaceutical product at the initial stage of microalgae growth, placing in a constant-temperature shaking table, and carrying out illumination culture. According to the method, different types of pharmaceutical products with different concentrations are added into the microalgae, the influence of the addition of the different types of pharmaceutical products with different concentrations on the growth promotion of the microalgae is analyzed, the biomass of the chlorella can be rapidly increased, and the method has important significance on the large-scale production of the microalgae.

A METHOD OF PRODUCING SINGLE CELL PROTEIN FROM WHEY

Nº publicación: WO2025256873A1 18/12/2025

Solicitante:

TRAKIA UNIV [BG]
TRAKIA UNIVERSITY

Resumen de: WO2025256873A1

The present invention is generally concerned with the field of microbial fermentation of dairy or dairy alterative industry waste material and microbial protein production such as single cell protein (SCP). In particular the present invention is directed to microbial fermentation of dairy derived or dairy alternative derived whey for production of single cell proteins (SCP) or microbial biomass. More in particular, the present invention relates to a method of producing a single cell protein or biomass, comprising propagating a microbial culture in a large scale setting under suitable conditions and growth medium comprising dairy-derived whey to produce a single cell protein, where the microbial culture comprises microalgae, yeast and bacterial species (Figure 1). The present invention also relates to method of identifying a strain of microorganism, or mixture of strains of microorganisms, capable of efficiently using dairy-derived whey waste as a food source to produce biomass as a source of single cell protein. The present invention also relates to a propagated microbial culture or a composition and use of the culture in microbial fermentation. The present invention further provides a product or supplement comprising the composition of the present invention.