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Myrmecia incisa MLDP gene knockout mutant strain as well as construction method and application thereof

Absstract of: CN122542385A

The invention belongs to the technical field of gene engineering and biology, and particularly relates to a Myrmecia incisa MLDP gene knockout mutant strain as well as a construction method and application thereof. The MLDP gene knockout mutant strain (delta MiMLDP) of Myrmecia incisa is obtained by performing targeted knockout on the MLDP gene of Myrmecia incisa through a CRISPR/Cas9 gene editing technology. Compared with a wild type, the obtained MLDP gene knockout mutant strain has a remarkably changed grease accumulation phenotype, can be used for grease production and biofuel preparation, can also be used for feed preparation, and can also be used as an additive or a carrier for preparation of food, cosmetics and medicines. Targeted knockout of the MLDP gene is achieved in Myrmecia incisa for the first time, a genetic material is provided for studying the function of MLDP in microalgae grease metabolism, and a new germplasm is also provided for creating microalgae with novel grease composition and lipid droplet structures.

Laminated automatic high-photosynthetic-efficiency microalgae reactor and application thereof

Absstract of: CN122542351A

The invention discloses a laminated automatic high-photosynthetic-efficiency microalgae reactor and application thereof, and relates to the technical field of microalgae reactors, the laminated automatic high-photosynthetic-efficiency microalgae reactor comprises a reactor support, a plurality of culture units are arranged on the reactor support, each culture unit comprises an illumination unit and a solid attachment medium, the solid attachment medium is obliquely arranged on the reactor bracket through the solid attachment medium holder, a nutrient solution supply system is arranged at one end of the solid attachment medium, and a solid attachment medium winding device is arranged at one end of the solid attachment medium. A plurality of culture units are arranged in a laminated manner through the reactor bracket, so that the yield per unit area is higher; the illumination unit improves the light utilization rate to a certain extent; the flexible solid attachment medium is used as a microalgae attachment site, and is curled and collected by the solid attachment medium winding device, so that the whole reactor production process has a relatively high automation level.

Automatic high-photosynthetic-efficiency microalgae reactor and application thereof

Absstract of: CN122542368A

The invention discloses an automatic high-photosynthetic-efficiency microalgae reactor and application thereof, and relates to the technical field of microalgae reactors, the automatic high-photosynthetic-efficiency microalgae reactor comprises a reactor support assembly, a plurality of conveyor belt gear shafts are rotatably arranged at the upper end and the lower end of the reactor support assembly, and the conveyor belt gear shafts are connected with a solid attachment medium through a transmission assembly; a nutrient solution supply assembly is arranged at a gear shaft of a conveying belt arranged at the upper end, a transmission assembly drives a solid attachment medium to sequentially flow through an inoculation area, an illumination culture area, a microalgae harvesting area, a cleaning and disinfecting area and an inoculation area, and a closed circulation loop is formed, so that automatic continuous production is realized; the adjacent conveyor belt gear shafts which are arranged in a vertically staggered mode enable solid attachment media to form inclined sections between the conveyor belt gear shafts, the multiple inclined sections are combined to enable the overall path of the media to be in a W-shaped winding shape, the microalgae attachment area on the unit occupied area is increased while microalgae attachment is guaranteed, and the yield is increased.

Method for efficiently synthesizing human selenoprotein P from microalgae

Absstract of: CN122542608A

The invention discloses a method for efficiently synthesizing human selenoprotein P from microalgae, and belongs to the field of bioengineering. Aiming at the problem that human selenoprotein P contains 10 selenocysteines and the translation readthrough efficiency is low due to multiple UGA termination codons in heterologous expression, the method comprises the following steps: performing codon optimization on a nucleotide sequence for coding the human selenoprotein P according to the codon preference of chlamydomonas reinhardtii; the method comprises the following steps: constructing a multi-copy expression vector containing two tandem expression cassettes, wherein each expression cassette is composed of an HSP70A-RBCS2 strong promoter, an optimized target gene and a terminator; transforming chlamydomonas reinhardtii, and screening a recombinant algal strain; culturing, adding a selenium source to induce expression, collecting algae strains, and purifying. Complete sequence expression of the human selenoprotein P containing 10 selenocysteine is realized in the chlamydomonas reinhardtii for the first time, the obtained protein has correct Sec doping and glycosylation modification, and the chlamydomonas reinhardtii is low in culture cost, free of endotoxin and suitable for production of food-grade or pharmaceutical-grade recombinant protein.

Device and method for clean ecological cultivation of haematococcus pluvialis by outdoor photobioreactor

Absstract of: CN122542346A

The invention discloses a device and a method for clean ecological cultivation of haematococcus pluvialis by an outdoor photobioreactor, and relates to the technical field of artificial cultivation of microalgae, and the method comprises the following steps: forming an annular raceway pond, creating an outdoor dust-free clean cultivation environment, arranging a partition plate, placing bundled waste glass tubes, and arranging a screen; a solid arc-shaped platform is arranged, alternanthera philoxeroides is planted, oocystis bogei is introduced for cultivation, and the oocystis bogei and zooplankton in the oocystis bogei form a food chain system; planktonic animals and plants and alternanthera philoxeroides in the water body are fully utilized by the crayfish, and waste generated by the crayfish enters the water body of the raceway pond to be recycled through material and energy circulation. The clean and ecological cultivation method is utilized, raised dust is reduced, the local light and temperature environment is improved, the influence of periodic breeding of various animals and plants on the land on microalgae production is reduced, large-scale efficient haematococcus pluvialis cultivation and crayfish cultivation integrated production is achieved, and the space utilization rate is increased.

Dehydration, drying, detoxification and deodorization device and method for bloom-forming cyanobacteria biomass after salvage

Absstract of: CN122542345A

The invention provides a dewatering, drying, detoxification and deodorization device and method for bloom-forming cyanobacteria biomass after salvage, the device comprises a rotating disc type photodegradation reactor, the rotating disc type photodegradation reactor adopts a double-layer structure, the upper layer of the double-layer structure is a photocatalytic degradation layer, and the lower layer is a biomass drying and dewatering layer; wherein a self-adaptive light degradation system is correspondingly arranged on the photocatalytic degradation layer, and a green electricity driven air drying system is arranged in the biomass drying and dewatering layer; the self-adaptive light degradation system carries out photocatalytic toxin degradation through switching of ultraviolet light and ultraviolet light in natural light, and the green electricity drives the air drying system to carry out drying treatment; the photocatalytic degradation layer is further provided with a microorganism cooperative treatment unit, and water in the photocatalytic degradation layer is detoxified, deodorized and cleaned through microorganisms in the microorganism cooperative treatment unit.

Method for synergistically degrading cyanobacteria algal toxin based on recombinant lactococcus lactis

Absstract of: CN122542580A

The invention belongs to the technical field of environmental microorganisms, and particularly relates to a method for synergistically degrading cyanobacteria algal toxin based on recombinant lactococcus lactis. Recombinant plasmids pNZ8148-MlrA and pAR1801-endolysin are constructed, the two plasmids are sequentially transformed into lactococcus lactis NZ9000 to obtain a double-plasmid engineering bacterium, cyanobacteria cells are cracked by endolysin through time sequence induction to release intracellular microcystic toxins, and the MlrA efficiently degrades toxins immediately. After the double-plasmid time sequence induction fermentation liquor is used for treatment for 12 hours, the algal toxin degradation rate reaches 99% or above, and the algal toxin degradation rate is obviously superior to that of single use of MlrA or endolysin and double-bacterium mixed treatment. The method disclosed by the invention realizes closed-loop treatment of first accumulating enzyme, then splitting decomposition release and then degrading toxin, has the advantages of high degradation efficiency, simplicity and convenience in operation, environment friendliness and the like, and has a wide application prospect in the field of cyanobacterial bloom treatment.

Bacteria-algae symbiotic artificial wetland system and operation method

Absstract of: CN122541024A

The invention provides a bacteria-algae symbiotic artificial wetland system and an operation method, and relates to the field of rainwater treatment and resource utilization. The algal-bacterial symbiotic artificial wetland system comprises a wetland bed body, a water inlet device, a water drainage device and a control unit. According to the system, an up-down partition structure and dry-wet alternate operation are adopted, the impact load resistance is effectively improved, and the energy consumption of the system is remarkably reduced through natural drying, emptying and reoxygenation. The algal-bacterial microspheres in the light-transmitting area avoid flora loss, in-situ oxygen production of microalgae supports symbiotic bacteria to realize high-efficiency denitrification, and low-carbon and stable purification of multi-element pollutants in complex runoff is finally realized by combining the synergistic interception effect of the filler at the bottom layer of the non-light-transmitting area.

Method for preparing agricultural microbial agent by utilizing biogas co-production culture phycomycetes

Absstract of: CN122542418A

The invention discloses a method for preparing an agricultural microbial agent by utilizing biogas co-production culture phycomycetes, and belongs to the technical field of ecological agriculture and waste recycling, biogas slurry and biogas of livestock and poultry manure biogas engineering are used as core raw materials, the pretreated biogas slurry is used as a basic culture medium, and the volume addition ratio is 10%-20%; inoculating stain-resistant microalgae and functional bacillus, wherein the initial biomass ratio is (3: 1)-(5: 1); introducing the desulfurized biogas into a culture system, and carrying out closed culture for 7-15 days under the conditions that the temperature is 22-28 DEG C, the illumination is 5000-8000 lx and the dissolved oxygen is 3-5 mg/L; after harvesting and concentrating, forming to obtain a powder or liquid agricultural microbial agent. According to the invention, the resource utilization rate of biogas is 100%, the culture cost is reduced by 60%, the activity of the microbial inoculum is improved by 30%, and the finished product meets the GB 20287-2006 standard.

Method for rapidly degrading domestic sewage polluted by thiamethoxam by utilizing microalgae fuel cell

Absstract of: CN122541008A

The invention discloses a method for rapidly degrading thiamethoxam-polluted domestic sewage by using a microalgae fuel cell, and relates to the technical fields of sewage treatment, novel pollutant biodegradation, microalgae resource utilization and microbial fuel cells. According to the method, a double-chamber microalgae fuel cell comprising an anode anaerobic power generation system and a cathode scenedesmus photosynthetic system is constructed, and an external circuit is kept closed under an illumination condition, so that electrons generated by an anode are transmitted to a cathode through the external circuit, and protons are migrated to a cathode chamber through a proton exchange membrane; meanwhile, photosynthetic oxygen production of the cathode scenedesmus is used as an electron acceptor to complete an oxygen reduction reaction. In the coupled system, scenedesmus grows, metabolizes and converts thiamethoxam by utilizing nutrient substances such as nitrogen and phosphorus in sewage, so that the biotransformation efficiency of thiamethoxam is accelerated under the driving of a closed external circuit, and the synergistic treatment effects of nitrogen and phosphorus removal, microalgae biomass accumulation and continuous bioelectricity output are synchronously realized.

Children composite neurotrophic composition based on intestinal brain axis regulation and low-temperature preparation process thereof

Absstract of: CN122515463A

The invention discloses a children composite nerve nutrition composition based on intestinal brain axis regulation and a low-temperature preparation process thereof, and relates to the technical field of special dietary nutrition. The composition is prepared from the following components in parts by mass: 38 to 52 parts of specific animal bifidobacterium subsp. Lactis BB-C04 bacterial powder, 22 to 35 parts of lactobacillus plantarum LP-N07 bacterial powder, 45 to 68 parts of isomaltooligosacharide IMO-900 type, 25 to 40 parts of palatinitol, 8 to 15 parts of high-purity yolk source phosphatidylserine PS-50 type and 12 to 22 parts of microalgae source docosahexaenoic acid grease microcapsule powder DHA-35 type. 6 to 11 parts of N-acetylneuraminic acid purified powder, 5 to 9 parts of L-alpha-glycerophosphorylcholine type GPC-50, 3 to 7 parts of natural source citicoline sodium salt and 0.8 to 1.6 parts of anti-caking agent silicon dioxide micro powder. The traditional Chinese medicine composition can significantly improve the symptoms of inattention, low learning and memory ability and gastrointestinal dysfunction coexistence of children of 3-12 years old.

Shellfish culture net carbon flux monitoring method, system and medium

Absstract of: CN122528107A

The invention relates to a shellfish culture net carbon flux monitoring method, a shellfish culture net carbon flux monitoring system and a medium. The method comprises the following steps: constructing a shellfish-microalgae co-culture unit and a microalgae contrast unit; synchronously and continuously acquiring heart rate signals of at least two shellfishes in the shellfish-microalgae co-culture unit to obtain effective heart rate data; hydrochemical parameters of the shellfish-microalgae co-culture unit and the microalgae control unit are obtained respectively; calculating to obtain net contribution data of the shellfish to the hydrochemical parameters according to the hydrochemical parameters; based on the effective heart rate data, calculating to obtain a heart rate cumulative average value; and calculating heart rate data collected in real time to obtain a real-time heart rate cumulative average value, substituting the real-time heart rate cumulative average value into the heart rate-hydrochemistry quantitative regression model, performing inversion to obtain a net contribution estimated value of shellfish to hydrochemistry parameters, and calculating to obtain the net carbon flux of a culture unit based on the total alkalinity net variation. According to the method, the shellfish physiological metabolism indexes and the water carbon chemical parameters are accurately associated, and the scientificity, the accuracy and the repeatability of the net carbon flux calculatio

Method for treating waste pit sealing mud of Maotai-flavor liquor based on thermal acid coupling microalgae process

Absstract of: CN122521404A

The invention discloses a method for treating waste pit sealing mud of Maotai-flavor liquor based on a hot acid coupling microalgae process, which comprises the following steps: air-drying the waste pit sealing mud, crushing, removing impurities, preparing into a suspension, introducing phosphoric acid, carrying out hot acid treatment under a heating condition, destroying a colloid structure, and promoting degradation-resistant macromolecular organic matters to be converted into soluble micromolecular organic matters; and transferring with nitrogen into the hydrolysate. After solid-liquid separation, a solid phase is recycled, supernatant rich in organic matter, free ammonia nitrogen and phosphorus is collected, the obtained supernatant makes up for phosphorus deficiency and shortage due to introduction of phosphoric acid, proper C: N: P balance can be achieved after simple dilution and adjustment by adding water, and the supernatant serves as the only culture medium to inoculate microalgae for culture. According to the method, deep coupling of pit sealing mud solid-phase repair and liquid-phase high-added-value microalgae biomass synthesis is achieved, and the method has the remarkable advantages that the process is compact, cost is reduced, efficiency is improved and the like.

ALGAE BIOMASS

Absstract of: US20260226398A1

The invention relates to an algae biomass having particular values within a defined colour space. The invention further relates to a strain of Chlorella microalgae having a genetic mutation in genes encoding for specific subunits of magnesium chelatase, preferably magnesium chelatase subunit, and also relates to a method of producing a strain of Chlorella microalgae having such mutations.

CARRIERS FOR GROWING ALGAE

Absstract of: US20260223795A1

0000 Microalgae are grown in a biofilm on carriers. In some examples, the carriers are surfaces with a small angle to the vertical. Optionally, the carriers may have surfaces on both sides of a vertical plane (e.g., an inverted V) or surrounding a vertical axis (e.g., a cone). In some examples, the carriers include two or more sheets of a porous material such as a fabric, screen or mesh. In some examples a surface of the carrier is flexible. In some examples, the carriers provide a specific surface area for algae growth greater than 7 m<2>/m<2>.

A GENETICALLY MODIFIED EUKARYOTIC MICROALGA AND INJECTABLE COMPOSITION FOR SYMBIOTIC THERAPY

Absstract of: WO2026163125A1

The present invention relates to a symbiotic therapeutic technique based on a genetically modified eukaryotic microalga (51), preferably photosynthetic, such as for example Chlamydomonas reinhardtii, in which an expression cassette (10) codifying a biologically active peptide and/or protein comprises a secretion coding module (5) of a therapeutic agent to allow the biologically active peptide/protein to be released so that it can cross the cell wall of the microalga (51), to be secreted outside of it and enter directly into the circulation in a subject (53) in which said genetically modified eukaryotic microalga (51) has been subcutaneously or intradermally injected. The microalga can be of a type that naturally: a) lacks flagella; or b) has flagella, in which case a prior genetic modification prevents the formation or inactivates the flagella, so that the microalga remains immobilised at a subject injection site (53). The expression cassette (10) can further comprise at least one further coding module selected from: a homing module (6), for targeting the peptide/protein to a predetermined tissue and/or cell population of the subject (53); a penetrating module (7) for inducing internalisation of the protein within a cell of said subject (53) through the cell membrane or by induced endocytosis; a protection module (9). The invention also relates to a subcutaneously or intradermally injectable composition containing said microalgae, and to a kit for preparing said composition.

COMPOSITION FOR MAINTAINING OR INCREASING FIBROUS STRUCTURAL PROTEIN

Absstract of: EP4785810A1

0001 An object of the present invention is to provide a formulation effective for maintaining or increasing hair fibrous structural proteins. Provided are a composition for maintaining or increasing fibrous structural proteins, a composition for maintaining or increasing skin elasticity, a composition for improving hair quality, and a composition for promoting production of collagen, elastin, or hyaluronic acid, which contain a culture supernatant and/or extracellular vesicles of a microalga.

Gas retention type partitioned carbon supplementing system for microalgae raceway pond and use method of gas retention type partitioned carbon supplementing system

Absstract of: CN122503201A

The invention discloses a gas retention type partitioned carbon supplementing system of a microalgae raceway pond and a using method, and belongs to the technical field of microalgae culture.The gas retention type partitioned carbon supplementing system comprises a raceway pond module and a carbon supplementing module, the carbon supplementing module is arranged in an algae liquid culture flow channel and connected with a gas buffer tank arranged outside the raceway pond module, the gas buffer tank is connected with a circulating gas pump, and the circulating gas pump is connected with the carbon supplementing module. And the circulating air pump is connected with a carbon-containing air source tank. The carbon supplementing module makes full contact with flowing algae liquid, unabsorbed tail gas is recycled, the utilization rate of carbon dioxide is increased, tail gas dissipation and pH fluctuation are reduced, and the carbon supplementing module is suitable for open or semi-open microalgae raceway ponds. The method is suitable for spirulina, chlorella, scenedesmus, dunaliella salina, haematococcus pluvialis, nannochloropsis oculata and other microalgae suitable for being cultured in an open or semi-open raceway pond; the method is especially suitable for large-scale microalgae culture and carbon dioxide emission reduction engineering taking pure carbon dioxide, air and carbon dioxide mixed gas, fermentation tail gas, industrial tail gas or coal-fired flue gas as a carbon s

Strip-shaped basalt fiber reinforced alginate-based microalgae immobilization material and preparation method thereof

Absstract of: CN122503372A

The invention discloses a strip-shaped basalt fiber reinforced alginate-based microalgae immobilization material and a preparation method thereof, and belongs to the technical field of environmental functional materials and algae biology. The preparation method comprises the following steps: cleaning basalt fibers, immersing the basalt fibers in a surface modifier, and drying the basalt fibers at a high temperature to form an active hydroxyl surface; immersing the modified basalt fiber in the algal glue solution, then transferring the algal glue solution into a calcium chloride aqueous solution for crosslinking, and repeating the steps until the thickness of a coating layer reaches a preset value; the algal glue solution is a mixed aqueous solution of sodium alginate and microalgae. The material disclosed by the invention is supported by a basalt fiber skeleton and is subjected to confinement coating by alginate gel, so that 1) the mechanical strength and the structural stability of an immobilization system are improved; the method has the advantages of simple operation, convenience in use, optimization of microalgae attachment and mass transfer environment, formation of a long-strip-shaped structure convenient for hanging, arrangement and overall recovery, direct resource utilization of the material after the use period, and preparation of the algae-based bio-fertilizer for improving saline-alkali soil.

Chlorophyll binding peptide and application thereof

Absstract of: CN122502449A

The invention discloses a chlorophyll binding peptide and application thereof, and belongs to the technical field of food science and biology. The invention relates to a chlorophyll binding peptide excavated from microalgae, in particular to a chlorophyll binding peptide with high chlorophyll binding activity. The chlorophyll binding peptide comprises a chlorophyll binding peptide PEP1, a chlorophyll binding peptide PEP2, a chlorophyll binding peptide PEP6 and a chlorophyll binding peptide PEP7. The chlorophyll binding peptide with low molecular weight shows unique advantages, has strong structural designability, realizes accurate interaction with chlorophyll, has better solubility and processing adaptability, and provides a new path for efficient protection of chlorophyll. The chlorophyll binding peptide obtained through screening can remarkably improve the thermal stability of chlorophyll in an aqueous solution through specific binding, and is suitable for natural pigment stabilization and functional food development.

Land-based round pool ecological breeding system and method for macrobrachium rosenbergii

Absstract of: CN122498449A

The invention discloses a macrobrachium rosenbergii land-based round pool ecological breeding system and method, and relates to the technical field of breeding. Comprising the steps of young shrimp putting, water quality management, bait casting management, pond reversing, young shrimp desalination and the like. The abundance of microalgae in a water body is maintained to be 104-107 cell/mL through micro-ecological regulation and control, a beneficial micro-ecological preparation is added, and water purification is realized by utilizing a micro-filter, a disinfection tank and a water purification tank; feeding management is adjusted according to the growth stage of shrimp seeds, feeding is not carried out in the I stage, artemia nauplii are mainly used in the II-VI stage, shrimp slices are gradually increased in the VII-XI stage, and egg custard and the shrimp slices are fed in the young shrimp stage; the pond pouring operation is carried out when ammonia nitrogen is greater than or equal to 2mg/L or nitrite is greater than or equal to 0.5 mg/L, and the salinity of the shrimp seeds is reduced to 0.3% by adding fresh water every day. The method is suitable for large-scale breeding, the survival rate and the growth speed of shrimp seeds are remarkably increased, and the method has the advantages of being efficient and ecological and can be applied to other aquaculture fields.

Pipeline-raceway pond series-connection microalgae large-scale collaborative culture process

Absstract of: CN122503223A

The invention discloses a large-scale microalgae collaborative culture process with pipelines and raceway ponds connected in series, and belongs to the technical field of microalgae biology. Comprising the following steps: step 1, carrying out sterile propagation on target microalgae species in a column type photobioreactor to obtain a primary microalgae species liquid; step 2, inoculating the primary algae seed liquid into a pipeline type photobioreactor for secondary propagation, executing gradient cooling domestication in the propagation process, and reducing the culture temperature to a target temperature range from an initial temperature range in stages to obtain secondary algae seed liquid; step 3, inoculating the secondary algae seed liquid into the open raceway pond according to a volume ratio of 10-30%, and carrying out semi-continuous culture; and step 4, collecting supernate harvested in the raceway pond, and recycling the supernate as at least part of water source for preparing the culture medium of the pipeline type photobioreactor after the supernate is treated. According to the process, the growth delay phase of the raceway pond after inoculation is shortened through gradient cooling domestication, the pollution failure rate of culture batches is reduced, and consumption of fresh water resources and nutritive salt is reduced through recycling of supernate.

SOD-rich algal-bacterial symbiotic liquid and preparation method thereof

Absstract of: CN122503253A

The invention relates to the technical field of agricultural planting, and provides a bacterial-algae symbiotic solution rich in SOD and a preparation method thereof, the pH value of the bacterial-algae symbiotic solution finished product is 4.4-4.6, and the bacterial-algae symbiotic solution is prepared from the following raw materials in parts by weight: 28-42 parts of an acid-resistant active microalgae solution, 18-26 parts of an acid-resistant composite functional bacterial solution, 32-46 parts of a weak acid organic symbiotic nutrient solution and 0.6-1.8 parts of an acid active stabilizer. Wherein the effective viable count in the algal-bacterial symbiotic liquid is greater than or equal to 2.2 * 10 CFU/mL, the viable microalgae retention rate is greater than or equal to 96%, and the SOD enzyme activity is greater than or equal to 900 U/mL. The invention solves the problems that the existing algal-bacterial symbiotic liquid cannot maintain the algal-bacterial dual-activity and low SOD enzyme activity under the condition of weak acidity (pH is about 4.5).

Intelligent automated microalgae cultivation system

Method of Cleaning Microalgae Culture Vessel

Nº publicación: KR20260118887A 31/07/2026

Applicant:

주씨엔에스아이엔티