Absstract of: CN120866027A
The invention belongs to the technical field of microalgae carbon sequestration, and particularly relates to a light energy weight self-adaptive microalgae internal and external light source complementary carbon sequestration system, which comprises a light culture assembly made of a transparent material and used as a container for containing algae liquid; the light supplementing assembly is arranged in the light culture assembly; the detection system is used for detecting illumination intensity data, illumination angle data, algae liquid biomass concentration data and light field distribution data in the light culture assembly; the control system is electrically connected with the detection system and the light supplementing assembly, and the control system adjusts the illumination intensity of the light supplementing assembly according to the illumination intensity data, the illumination angle data, the light field distribution data and the algae liquid biomass concentration data. According to the system, the light quality and the light intensity of a light source are accurately regulated and controlled by predicting the light field distribution in the light culture assembly and adjusting the light supplementing intensity of the light supplementing assembly, so that light field reconstruction is realized, stable carbon sequestration of microalgae is assisted, and high-quality target carbon neutralization is realized.
Absstract of: CN120866369A
The invention belongs to microalgae bioengineering, and particularly relates to an MCP transgenic expression vector and application thereof. The invention provides an MCP recombinant plasmid, the MCP recombinant plasmid is transferred into microalgae for the first time, the microalgae is not only an initial feed but also can be used as an expression vector of an exogenous gene, and the MCP gene of DIV1 is used for preparing a microalgae oral vaccine, so that the vacancy of a medicine for preventing and treating the full-eye iridovirus 1 at present is made up, the homology of medicine and food is realized, and the aim of preventing and treating the full-eye iridovirus 1 is fulfilled. The invention provides an environment-friendly, safe and effective transgenic microalgae vaccine drug, promotes the development of an aquatic vaccine technology, and has far-reaching significance on the stable and sustainable development of global aquaculture industry.
Absstract of: CN120866072A
The invention discloses a culture medium and a culture method for inhibiting microalgae sensitization protein expression. The culture medium comprises the following components: an organic carbon source, a nitrogen source, a phosphorus source and other elements, the culture method comprises the following steps: inoculating algae liquid activated to a logarithmic phase into the culture medium for continuous culture, and adjusting the temperature, illumination intensity, light-dark cycle and culture period of the closed photobioreactor for culture. According to the culture medium composition, element matching of the culture medium is ingeniously coordinated, chromatin remodeling and metabolism reprogramming of the microalgae can be effectively influenced, then carbon flow distribution in glycolysis and tricarboxylic acid circulation is changed, energy metabolism and growth balance are rebuilt, and finally inhibition of sensitization protein expression of the microalgae is achieved. On the basis, microalgae sensitization risk factors are determined, and the expression degree of microalgae sensitization protein is reduced by changing the culture condition combination, so that the edible safety of microalgae is improved.
Absstract of: CN120866074A
The invention belongs to the technical field of microalgae biology, and in particular relates to thaurita procumbens and a method for producing fucoxanthin by utilizing the thaurita procumbens. The invention relates to a phalaenopsis palustris which is named PCSM-1 and is preserved in the China Center for Type Culture Collection on January 11, 2024, and the preservation number is CCTCC NO: M 2024076. The content of fucoxanthin in the brachythecium cutescens is as high as 1.58 wt%, and the brachythecium cutescens can grow under both a photoautotrophic condition and a heterotrophic condition. The method comprises the following steps: firstly, performing heterotrophic fermentation on the palmaris cutescens so that the palmaris cutescens is rapidly proliferated and fermented; the method comprises the following steps: firstly, carrying out photoautotrophy and heterotrophy induction to quickly accumulate the fucoxanthin, so that the whole process is short in period and low in energy consumption, the yield of the fucoxanthin is far higher than that of the existing microalgae, and the problems of low production efficiency, high cost and the like of the current fucoxanthin can be solved.
Absstract of: CN120866149A
The invention discloses a cyanobacteria bottom mud fermentation methanogenic bacterial agent, which comprises clostridium butyricum, bacillus amyloliquefaciens and methanosarcina martensii, and is characterized in that the clostridium butyricum accounts for 30-60% of the total number of bacterial cells, the bacillus amyloliquefaciens accounts for 25-50% of the total number of the bacterial cells, and the methanosarcina martensii accounts for 30-60% of the total number of the bacterial cells. The methanosarcina marzeberi accounts for 10%-25% of the total number of bacterial cells. The advanced technology of adding microbial compound bacteria and combining high-temperature and high-pressure hydrolysis and anaerobic digestion technologies is utilized, the biodegradability is improved by destroying the cell structure of organic matter, the efficiency and stability of traditional anaerobic fermentation are remarkably improved, the VS removal rate after pyrohydrolysis fermentation reaches 67.5%-70.4%, the gas production rate in the anaerobic fermentation process after pyrohydrolysis is effectively increased, and the production cost is reduced. The yield of methane can be increased, the water body environment can be improved, recycling of bottom mud resources can be promoted, and green and sustainable ecological restoration is achieved.
Absstract of: CN120866251A
The invention discloses a beta-carotene hydroxylase mutant as well as a preparation method and application thereof, and belongs to the technical field of microalgae gene engineering. Amino acid Cys No. 191 of haematococcus pluvialis beta-carotene hydroxylase is mutated into Ala, amino acid Thr No. 213 of haematococcus pluvialis beta-carotene hydroxylase is mutated into Ala, and the yield of astaxanthin is increased by 1.282 times and 1.268 times respectively. According to the mutant obtained through mutation, the catalytic efficiency and substrate selectivity of beta-carotene hydroxylase are remarkably improved, astaxanthin is produced more specifically through enzymatic reaction, and a key mutation direction is provided for construction of engineering strains for high yield of astaxanthin.
Absstract of: CN120866071A
The invention relates to the technical field of microalgae culture, and discloses a microalgae culture method. The invention relates to a microalgae culture method, which comprises the following steps: (1) in the presence of a fat degradation inhibitor, culturing microalgae species to obtain a microalgae solution; (2) separating, adsorbing and degerming the algae solution to obtain a culture solution; and (3) returning the culture solution to the step (1) for reuse. The method can effectively control the generation of organic matters with a growth inhibition effect in the microalgae culture process, improve the production rate and reduce the culture water consumption.
Absstract of: CN120866183A
The invention discloses an infrared light driven microalgae photosynthetic carbon sequestration method and application, and belongs to the technical field of photosynthetic organism culture.The method comprises the steps that a near-infrared up-conversion nanometer material is dispersed in a microalgae culture medium, and a full-spectrum or near-infrared light source is used for promoting microalgae photosynthetic carbon sequestration; the near-infrared up-conversion nano material is a rare earth nano material with a NaYF4 inert shell structure. According to the method, the overall sunlight utilization efficiency of the microalgae is improved, and the photosynthetic carbon sequestration capacity of the microalgae is finally improved.
Absstract of: CN120866073A
The invention discloses a high-efficiency biological carbon sequestration method and system device for coupling culture of microalgae based on flue gas and by-products after ammonia decarburization, and the method is characterized in that the flue gas (the temperature is 25-35 DEG C, and the CO2 concentration is 1-5%) after the ammonia decarburization is directly used as a microalgae carbon source, and the by-product ammonium sulfate (the adding concentration in a culture medium is 0.1-2.5 g/L) after the ammonia decarburization is used as a nitrogen source; ammonium bicarbonate (the addition concentration in the culture medium is 0.1-2.5 g/L) is used as a nitrogen source and an auxiliary carbon source, microalgae are inoculated into the prepared culture medium, the flue gas decarbonized by the ammonia process is directly introduced, and photosynthetic culture is carried out. The pH value of a culture medium is monitored in real time, the flow of flue gas obtained after ammonia decarburization and the adding proportion of ammonium bicarbonate and ammonium sulfate are cooperatively fed back and regulated, the pH of a culture system is kept stable, carbon and nitrogen sources are sufficient, high-precision stable control over the culture environment is achieved, and efficient biological carbon sequestration is achieved.
Absstract of: CN120870119A
The invention provides an in-situ online observation device applied to a microalgae growth dynamic response process. The in-situ online observation device comprises an in-situ online observation device and an automatic shooting system, the in-situ online observation device comprises a culture observation plate, a mainstream micro-channel and a micro-culture chamber; the culture observation plate is transparent; the mainstream micro-channel is arranged on the upper side of the culture observation plate; a flow inlet and a flow outlet are respectively formed in two ends of the main flow micro-channel in the length direction; the plurality of micro-culture chambers are communicated with the main flow micro-channel; the plurality of micro-culture chambers are arranged between the flow inlet and the flow outlet; the automatic shooting system is used for shooting microalgae in the micro-culture chamber; the in-situ online observation device is simple and effective in structure and convenient to manufacture, and has the characteristics that internal nutrients and metabolic oxygen are efficiently transported and transferred, and the nutrients are continuously, stably and uniformly distributed; and in combination with an automatic shooting system, long-time continuous imaging observation can be carried out on the intrinsic dynamic response process of the growth of a plurality of microalgae cells, and theoretical guidance is provided for the maximum growth potential of the microalgae c
Absstract of: WO2025224659A1
The present disclosure relates to an apparatus for microalgae and/or cyanobacteria cell culture comprising a unitary body formed from a hydrophobic material; and two or more microcapillary channels extending along the unitary body; wherein the inner surface of the microcapillary channels is coated with nutrients suitable for microalgae and/or cyanobacteria cell culture; and wherein the hydrophobic material is transparent to visible light, and to a kit and sensor thereof. A method for culturing microalgae and/or cyanobacteria in an apparatus comprising a unitary body formed from a hydrophobic material and two or more microcapillary channels extending along the unitary body, wherein the hydrophobic material is transparent to visible light is also disclosed.
Absstract of: WO2024132882A1
The invention provides marine microalgal strain of the Ulvales order of the Ulvophyceae class which have utility in the efficient and environmentally beneficial preparation of triacylglycerides and polyunsaturated fatty acids, which can in turn be used for fuels or feeds.
Absstract of: WO2024133379A1
The invention relates to a method for preparing a cyanobacterium extract, preferably spirulina, comprising phycocyanin enriched in trace elements. The invention also relates to an extract obtained by the method according to the invention, to a composition comprising said extract and to the use thereof.
Absstract of: CN120843118A
The invention relates to the technical field of crust preparation preparation, in particular to a crust biological preparation, a preparation method and application thereof, and the preparation method comprises the following steps: S1, preparing a bacillus modifier; s2, preparation of a microalgae-biopolymer mixed solution; s3, primary compounding; and S4, compounding to form the agent. Through the synergistic effect of the bacillus modifier and the microalgae-biopolymer mixed solution, the biological crust forming period is remarkably shortened, the defect that natural recovery is slow (dozens of years) is overcome, meanwhile, the crust forms a compact net-shaped structure and strongly bonds soil particles, and the wind erosion rate is reduced by 50%. The freeze-drying process is improved, the biological activity is better reserved, the stability of the preparation is improved, the preparation is convenient to store and transport, the powder which is crushed and sieved (100 meshes) can be directly scattered, and field planting is further accelerated.
Absstract of: CN120841711A
The invention discloses a purification method of breeding wastewater, and relates to the field of wastewater treatment.The purification method comprises the following steps that straw is taken to be subjected to enzymolysis to prepare straw hydrolysate, and the straw hydrolysate, microalgae and spores of fungi are added into the breeding wastewater to be subjected to oscillation co-culture; the microalgae comprise chlorophyta algae, and the fungi comprise aspergillus fungi. According to the method, a phycomycetes purification system is formed in the sewage through the chlorophyta and the aspergillus fungi, the straw hydrolysate is matched to provide nutritional ingredients such as saccharides and amino acid for phycomycetes, the stability degree of the content of the nutritional ingredients in the sewage is improved, and under the comprehensive action, the removal rate of ammonia nitrogen and total phosphorus in the sewage is increased, and the ammonia nitrogen and the total phosphorus are converted into biomass.
Absstract of: CN120843281A
The invention relates to the technical field of algae culture, in particular to a microalgae expanding culture method, a microalgae expanding culture system and an ecological restoration method. The microalgae expanding culture method comprises the following steps: inoculating a microalgae seed solution into a first culture solution, and carrying out first amplification treatment under the illumination condition of 100-500lux to prepare a first algae seed mixed solution; inoculating the first algae seed mixed solution into a second culture solution, and carrying out second amplification treatment under the illumination condition of 1000-3000 lux to prepare a second algae seed mixed solution; inoculating the second algae mixed solution into a third culture solution, and performing third amplification treatment at the pH value of 6.5-10 to prepare a third algae mixed solution; inoculating the third algae mixed solution into a fourth culture solution, and carrying out fourth amplification treatment under a stirring condition to prepare a fourth algae mixed solution; wherein the volumes of the first culture solution, the second culture solution, the third culture solution and the fourth culture solution are sequentially increased. According to the expanding culture method, the microalgae grow fast, and the culture period can be effectively shortened.
Absstract of: CN120841600A
The invention relates to a microalgae culture solution recycling method, which comprises: carrying out solid-liquid separation on a microalgae culture solution to obtain a filtrate; enabling the filtrate to pass through a resin column and collecting effluent; the effluent is optionally mixed with a supplement and then returned for microalgae culture. By adopting the method for recycling the microalgae culture solution, wastewater discharge in the microalgae culture process can be effectively reduced, the wastewater biochemical treatment pressure is reduced, after the treated effluent is recycled for microalgae culture, the higher microalgae biomass content can still be obtained, the water consumption of microalgae production is remarkably reduced, and the method has a good application prospect.
Absstract of: CN120829860A
The invention discloses a bacterial strain for degrading aspartame, a bacterial-algae symbiotic system and application of the bacterial strain and the bacterial-algae symbiotic system, the bacterial strain is corn sphingomonas zeae NCU J1, and the preservation number of the bacterial strain is CCTCC (China Center for Type Culture Collection) NO: M 20251462. And an algal-bacterial symbiotic system is constructed by adopting an oriented symbiotic combination of the corn sphingomonas NCU J1 and the chlorella sp. The corn sphingomonas NCU J1 can metabolize an artificial sweetening agent aspartame and degradation products thereof, metabolism complementation can be achieved through an algal-bacterial symbiotic interface, and bacteria preferentially promote aspartame to be subjected to photohydrolysis to generate degradation products such as diketopiperazine derivatives. The microalgae synchronously start isomerism enzymatic reaction to convert degradation products such as diketopiperazine derivatives into low-toxicity products, the risk of secondary pollution is reduced by 40% or above compared with that of a traditional physical and chemical method, 150 mg/L aspartame can be efficiently degraded within 2 days, generation of derivatives of aspartame can be greatly reduced, secondary pollution is reduced, and the method is suitable for industrial production. And the method has a good application prospect in the aspect of rapidly treating aspartame-containing wastewater.
Absstract of: CN120835926A
The present invention provides strains of marine microalgae of the ulva lactuca, which can be used for the efficient and environmentally beneficial preparation of triacylglycerides and polyunsaturated fatty acids, which in turn can be used in fuels or feeds.
Absstract of: CN120827149A
The invention belongs to the technical field of bioengineering and the field of sewage treatment, and particularly relates to a method for efficiently treating livestock and poultry biogas slurry to prepare microalgae protein bait by coupling aquatic vegetables with microalgae, which comprises the following steps: carrying out primary treatment on livestock and poultry wastewater by utilizing the aquatic vegetables, and carrying out secondary treatment on the microalgae with high robustness by coupling; the pollutant content of the biogas slurry is reduced, microalgae protein bait is harvested, and pollutants such as COD content, ammonia nitrogen content and phosphorus content in the livestock and poultry biogas slurry are efficiently reduced. According to the method, the pollutant content of the livestock and poultry biogas slurry is efficiently reduced, the COD content of the treated biogas slurry is reduced by 75%-90%, the ammonia nitrogen content is reduced by 80%-92%, the phosphorus removal rate reaches 90% or above, the biomass of microalgae harvested at low cost through self-flocculation reaches 2 g/L or above, the protein content in the microalgae reaches 50%-70%, the content of essential amino acids in the microalgae is close to that of fish meal, and the content of organic matters in the microalgae is reduced by 50%-70%. The feed can be used as high-quality protein bait.
Absstract of: WO2025221466A1
The invention relates to a method of producing a phototrophic cyanobacteria based biofertilizer.
Absstract of: US2025325609A1
Provided is a composition comprising microalgae and clay, formulated for ingestion, application to skin, an oral cavity, or administration rectally to a mammal. Also provided is a method of treating a mammal. The method comprises administering the above composition to the mammal.
Absstract of: US2025327096A1
A method for enhancing growth of a microalgal species and its biomass production yield by co-cultivating the microalgal species with a bacterial isolate under the genus of Pseudoalteromonas in separate compartments. The microalgal species is selected from Dunaliella species. The genome sequence of the Pseudoalteromonas isolate can have a nucleotide similarity of lower than 95% to that of other Pseudoalteromonas species.
Absstract of: FI20255069A1
The present approach describes continuous, steady state algal aquaculture systems, methods, and uses. Algal aquaculture systems may employ a plurality of algal aquaculture stages, first to grow the microalga of interest, and subsequent stages for subjecting the microalga of interest to controlled stress. Algae biomass composition in the last algal aquaculture stage can be selectively determined through various process elements to impact product generation and/or to reduce the recalcitrance of the algal biomass towards downstream processing, such as fracking the cells to enable chemical-free harvesting via, e.g., adsorptive bubble technology.
Nº publicación: FI20255068A1 22/10/2025
Applicant:
NESTE OYJ [FI]
Neste Oyj
Absstract of: FI20255068A1
The present approach describes continuous, steady state algal aquaculture systems, methods, and uses. Algal aquaculture systems may employ an algal aquaculture zone to grow the microalga of interest, and a collector for receiving a continuous stream of algal aquaculture medium for fracking and harvesting.