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Chemical sterilization or disinfection method of microalgae culture medium using calcium hypochlorite

Resumen de: KR20250122828A

본 발명은 차아염소산칼슘(calcium hypochlorite, Ca(ClO)2)을 이용한 미세조류 배양 배지의 화학적 멸균방법에 관한 것으로, 차아염소산칼슘(calcium hypochlorite, Ca(ClO)2)을 미세조류 배양 배지에 넣어 멸균한 후 MnCl2-Na2EDTA 복합체 및 glucose의 촉매 반응을 통해 차아염소산칼슘을 중화시켜 멸균된 미세조류 배양 배지에 의해 미세조류의 성장을 증대시키는 것이 특징이다. 상기 본 발명에 따른 미세조류 성장의 증대는 영양 보충제, 식품 착색제, 사료 첨가제 및 성장 촉진제, 폐수 처리제, 바이오 연료 등 다양한 산업 분야에 활용될 수 있다.

Method for enhancing microalgae carbon sequestration through cooperation of seaweed-based carbon quantum dots and plant hormones

Resumen de: CN120459796A

The invention relates to a method for enhancing microalgae carbon sequestration through cooperation of seaweed-based carbon quantum dots and plant hormones, and belongs to the technical field of biological carbon sequestration. According to the method, chlorella is used as a carbon sequestration carrier and is cultured in a vertical tubular photobioreactor, indole-3-acetic acid (IAA) and seaweed-based carbon quantum dots (U-CQDs) are added in an initial stage, and culture conditions (25-30 DEG C, 3000 Lux illumination and 15% CO2 supply) are optimized. The seaweed-based carbon quantum dots (U-CQDs) are prepared by taking enteromorpha prolifera as a raw material through a hydrothermal method, and have excellent light conversion characteristics. Through the synergistic effect of the U-CQDs and the IAA, the light energy utilization efficiency, the CO2 fixation rate and the biomass accumulation of the microalgae are remarkably improved, and meanwhile, the culture energy consumption is reduced. The method is easy and convenient to operate, low in cost and suitable for efficient capture and resource utilization of industrial flue gas CO2.

Microalgae membrane photobioreactor for treating artemia culture tail water

Resumen de: CN120463348A

The present invention relates to the technical field of wastewater treatment, and provides a microalgae membrane photobioreactor for artemia culture tail water treatment, the microalgae membrane photobioreactor comprises a transparent reactor shell, and the inner wall, close to the bottom, of the transparent reactor shell is fixedly connected with a flat sheet membrane. According to the invention, the motor is controlled by the controller to start, so that the threaded strip can move towards one side along the outer surface of the reciprocating screw rod, teeth of two racks on one side can be continuously meshed with teeth of corresponding gears, two gears can rotate, the sealing state of the sealing cylinder to the transparent reactor shell is gradually released, the sealing cylinder stops rotating, and the transparent reactor shell is sealed. Through reciprocating movement of a scraping strip, algal mud on the polyester non-woven fabric can be pushed into two sealing cylinders, so that the algal mud attached to the polyester non-woven fabric can be scraped and discharged from the interior of the transparent reactor shell, manual treatment is not needed in the process, time and labor are saved, and the efficiency is improved. Therefore, the artemia culture tail water treatment efficiency is improved.

Selenium-rich DHA-rich feed additive as well as preparation method and application thereof

Resumen de: CN120458196A

The invention provides a selenium-rich DHA-rich feed additive as well as a preparation method and application thereof, and belongs to the technical field of feed additives. The preparation method comprises the following steps: preparing a core material: dissolving sodium alginate in water, adding schizochytrium limacinum powder and water-soluble selenoprotein, heating and mixing to obtain a mixed solution 1; adding the mixed solution 1 into the rapeseed oil, adding polysorbate, and mixing to obtain a mixed solution 2; adding a calcium chloride solution into the mixed solution 2, centrifuging, collecting precipitate, and drying to obtain a core material; preparing a wall material: thermally melting corn viscose, then adding maltodextrin and polycaprolactone, and mixing to obtain the wall material; and coating: mixing the core material and the wall material for coating. The method is simple and easy to operate, the rancidity rate of DHA in the core material can be inhibited, the stability of DHA microalgae powder in stomachs and intestines of livestock and poultry is improved, and the conversion rate is increased. And meanwhile, the water-soluble selenoprotein in the core material and the microalgae powder rich in DHA have a synergistic effect, so that the contents of selenium and DHA in livestock and poultry products can be effectively increased.

Microalgae particle incubator and system for carbon capture

Resumen de: CN120464467A

The invention provides a microalgae particle culture device and system for carbon capture, the microalgae particle culture device for carbon capture comprises a tank body, a first guide cylinder and a second guide cylinder, the tank body is provided with a bottom water inlet pipe and an upper overflow port, the first guide cylinder is coaxially arranged in the middle of the tank body, and the second guide cylinder is coaxially arranged in the middle of the tank body; the bottom of the water inlet pipe is opposite to the water inlet pipe and forms an overflow gap with the bottom of the tank body, and a flow guide component positioned below the overflow port is arranged above the top and forms an overflow gap with the top; the top of the second guide cylinder is fixed with the top wall of the tank body, the bottom of the second guide cylinder sleeves the periphery of the first guide cylinder, and overflowing gaps are formed among the second guide cylinder, the inner wall of the tank body and the first guide cylinder; and a lighting assembly is arranged in at least one of the tank body, the first guide cylinder or the second guide cylinder. According to the application, efficient particle culture, convenient harvesting and carbon sequestration are realized by constructing a stable backflow channel, inducing particle deposition, promoting microalgae suspension flow and particle aggregation, providing directional light supplement by the lighting assembly, improving the photosynthe

Composite microbial agent for treating cyanobacterial bloom

Resumen de: CN120464546A

The invention relates to the technical field of microbial engineering, and particularly discloses a compound microbial agent for treating cyanobacterial bloom, bacillus velezensis provided by the invention destroys algae cells and inhibits the activity of related biological enzymes by secreting heterocyclic algal killing active substances, and simultaneously causes the leakage of cellular contents (such as algal toxins, polysaccharides and lipids); an extracellular enzyme system (such as alkaline phosphatase and beta-glucosidase) of the sphingomonas decomposes free algal toxins into low-toxicity linear peptide fragments and hydrolyzes beta-1, 4-glucosidic bonds in EPS at the same time, so that algae-derived organic fragments are converted into small-molecular soluble substances, and a substrate is provided for follow-up metabolism of rhodococcus; lipase and esterase secreted by rhodococcus can hydrolyze triglyceride and waxy components, fatty acid is converted into energy and a biosurfactant through a beta-oxidation pathway, and hydrophilicity of frond fragments is enhanced to promote overall degradation efficiency.

Integrated extraction device for microalgae polysaccharide

Resumen de: CN120442390A

The invention relates to an integrated extraction device for microalgae polysaccharide. The integrated extraction device comprises an algae adding system, a centrifugal system, an enzymolysis system, a flash extraction system, a separation and purification system and a drying system, the centrifugal system comprises a centrifugal device, a filtering device, a slag scraping device, a feeding hole and a discharging hole; the enzymolysis system comprises an enzyme adding tank, an enzymolysis tank, a reflux system, a filtering system, a water adding system and a monitoring and safety system; the flash extraction system comprises a flash extraction device, a lifting device, a fixing device, a feed port and a discharge port; the separation and purification system comprises a solid-liquid separation unit, a deproteinization unit, a decolorization unit, an alcohol precipitation unit and a purification unit; the drying system comprises an atomization device and a hot air device and can dry microalgae polysaccharide. The extraction device can realize automatic and efficient extraction of microalgae polysaccharide, is automatic, efficient and suitable for various microalgae, and the microalgae polysaccharide prepared by adopting the system has relatively good stability, biological activity, extraction rate and purity.

Full-circulation fishery breeding water treatment device and method based on photocatalytic microalgae coupling

Resumen de: CN120441090A

The invention provides a full-circulation fishery breeding water treatment device and method based on photocatalytic microalgae coupling, and the device mainly comprises a photocatalytic treatment pond, an adult breeding pond, a seedling breeding pond and a microalgae bait breeding pond, an anode, a cathode, a photocatalytic film, deposited bottom mud, an ultraviolet lamp, a CO2 aeration disc, a gate valve, a water pump, an LED light source and a water quality sensor are also arranged in different tanks. An anode, a cathode, a water body and an external circuit in the full-circulation fishery breeding water treatment device jointly form a bacteria-algae biological fuel cell which supplies power to equipment such as an ultraviolet lamp of a photocatalysis treatment pond, an LED light source of each breeding pond, a water pump and the like; a water quality sensor network is formed in the full-circulation water treatment device, and the processes of water supplementing, bait supplementing and the like are efficiently controlled in time. According to the invention, the photocatalytic oxidation and the biological fuel cell cooperate to purify culture sewage, the microalgae is used as bait to reduce cost and improve immunity, and the microbial fuel cell generates power to supply oxygen to the microalgae light source, so that zero emission, full circulation and water saving are realized.

Spirulina with anti-pollution capacity and application thereof

Resumen de: CN120442472A

The invention relates to spirulina with anti-pollution capacity, which is preserved in China General Microbiological Culture Collection Center on December 25, 2024, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.46270. The spirulina strain provided by the invention has remarkable anti-pollution capacity, and can effectively resist various external pollution in an open culture environment, such as interference of harmful microorganisms, germs and other harmful substances. The algal strain also has strong environmental adaptability, can stably grow in different water quality conditions and temperature ranges, and ensures the sustainability of production and culture. The algal strain can produce various bioactive substances at high yield, and can be widely applied to industrial production in the fields of microalgae-based feed additives, health-care products, food, cosmetics and the like. In general, the spirulina strain disclosed by the invention, as a high-quality algae species, has a remarkable stress resistance advantage, so that not only is the production efficiency improved, but also the technical progress and healthy development of related industries are promoted.

Method for promoting microalgae growth by using pharmaceutical product

Resumen de: CN120442514A

The invention belongs to the technical field of microalgae biology and culture, and particularly relates to a method for promoting microalgae growth by using a pharmaceutical product, which comprises the following steps: activating microalgae on a solid flat plate, inoculating the activated microalgae into a liquid culture medium, adding the pharmaceutical product at the initial stage of microalgae growth, placing in a constant-temperature shaking table, and carrying out illumination culture. According to the method, different types of pharmaceutical products with different concentrations are added into the microalgae, the influence of the addition of the different types of pharmaceutical products with different concentrations on the growth promotion of the microalgae is analyzed, the biomass of the chlorella can be rapidly increased, and the method has important significance on the large-scale production of the microalgae.

Method for screening spirulina with anti-pollution capacity

Resumen de: CN120442748A

The invention relates to a method for screening spirulina with anti-pollution capacity, which comprises the following steps: S1, inoculating starting spirulina into a liquid culture medium, and adding a pollution system to obtain a bacterium-algae evolution screening system; s2, culturing the bacteria-algae evolution screening system under illumination until algae cells are basically split; s3, resuscitating the bacteria-algae evolution screening system to obtain a resuscitated spirulina culture; and S4, purifying the spirulina with anti-pollution capacity from the spirulina resuscitation culture. On the basis of biological stress of polluting bacteria in a microalgae culture system, adaptive evolution of the spirulina on different culture scales is promoted by utilizing the algae lysing effect of the mixed bacterial liquid on the spirulina and evolution rescue of the spirulina, and finally, the spirulina strain with higher stress resistance and pollution resistance is obtained by utilizing the existing spirulina strain. And a rapid and effective way is provided for spirulina breeding.

High-yield glucose-secreting cyanobacteria and application thereof

Resumen de: CN120442677A

The invention relates to application of a cyanobacteria glucokinase inactivating agent in preparation of cyanobacteria with a glucose secretion function or high glucose yield. The invention further relates to cyanobacteria with a high glucose secretion function or high glucose yield and application of the cyanobacteria. Glucokinase in the cyanobacteria loses functions. According to the invention, glucokinase of cyanobacteria is knocked out, the mutant strain accidentally obtains the capacity of mass production of glucose and secretion of glucose to the outside of cells, the yield of glucose is increased by hundreds of times, the mutant strain provides possibility for subsequent industrial production of glucose by using cyanobacteria, and the mutant strain can be used for constructing a biological photovoltaic device, and has a wide application prospect. And glucose is continuously provided for the cathode cell and is used for being matched with a glucose oxidation electron transfer chain displayed extracellularly to generate reducing power and electrons, so that the light energy conversion efficiency is improved, and efficient transmembrane transfer of energy substances is realized.

Livestock and poultry breeding wastewater treatment device based on dynamic bacterial-algae biological membrane and method and application of livestock and poultry breeding wastewater treatment device

Resumen de: CN120441104A

The invention discloses a livestock and poultry breeding wastewater treatment device based on a dynamic bacterial-algae biological membrane and a method and application thereof, and relates to the field of livestock and poultry breeding tail water treatment. According to the biological treatment membrane bed unit, biological treatment and evaporation processes are creatively combined, and the system stability and the wastewater purification efficiency are remarkably improved. The unit is high in environmental adaptability and free of emission and peculiar smell during operation, the wastewater volume is effectively reduced, and the limitation of resource utilization of aquaculture wastewater is broken through. The biological membrane contains a plurality of microorganisms, including high-efficiency microorganisms added at the early stage and microorganisms captured from the environment, so that the operation and maintenance cost of equipment is reduced. Microalgae cells grow in the form of a biological membrane and are easy and convenient to harvest, and automatic continuous production is realized. According to the technology, organic waste in the breeding wastewater is recycled, the product is widely applied to multiple fields, the biological carbon sequestration function is achieved, and the carbon trapping device is low in cost, high in output value, green and environmentally friendly and assists in constructing a sustainable technical system and a circular economy mode.

Method for generating and extracting microalgae metabolite

Resumen de: CN120442720A

The invention discloses a microalgae metabolite generation and extraction method. The microalgae culture is subjected to stress treatment to stimulate the microalgae to increase the secretion of metabolites. In addition, cell walls of the microalgae are decomposed by hydrolyzing enzymes or fungal cultures, so that metabolites of the microalgae are released more effectively. And finally, separating and purifying the metabolite released by the microalgae by adopting methods such as filtration, centrifugation, ultrafiltration and the like. According to the method, the yield of microalgae metabolites can be increased, and the method has important value for application of microalgae in the fields of biotechnology, food, medicine industry and the like.

Seawater chlorella with high ammonia nitrogen removal rate and high starch accumulation capacity as well as culture method and application thereof

Resumen de: CN120442407A

The invention discloses seawater chlorella with high ammonia nitrogen removal rate and high starch accumulation capacity as well as a culture method and application of the seawater chlorella. The problems that the growth rate is reduced and the ammonia nitrogen treatment efficiency is reduced due to the salt stress effect when a traditional bioremediation technology is applied to a tidal basin are solved. According to the method, the seawater chlorella with high adaptability is utilized, the ammonia nitrogen absorption capacity and the starch accumulation amount of the seawater chlorella are remarkably improved through the salt synergistic effect in combination with the immobilization technology, and therefore effective treatment on the ammonia nitrogen pollution of the tidal basin is achieved. Under the conditions that the salinity is 0.5% and the ammonia nitrogen concentration is 3 mg/L, the ammonia nitrogen removal rate of the seawater chlorella reaches 93.60%, the starch accumulation amount reaches 10.06 mu g/10 < 6 > cells, and the ammonia nitrogen removal rate and the starch accumulation amount are increased by 23.2% and 58.4% respectively. The method has the advantages of simple and convenient process, high efficiency, strong environmental adaptability and the like, and is suitable for ammonia nitrogen pollution treatment and microalgae resource utilization in tidal basins.

Microalgae culture, harvesting and energy utilization process and system

Resumen de: CN120442405A

The invention relates to the field of microalgae culture and energy utilization. More specifically, the invention relates to a microalgae culture, harvesting and energy utilization process and system, cultured microalgae are harvested in a flocculation mode, ferric nitrate is used as a flocculant, ammonia water is added to adjust pH, generated ammonium nitrate can be recycled as a nitrogen source, and iron ions are changed into ferric hydroxide precipitates to be removed; the harvested microalgae is subjected to pyrolysis to prepare high-calorific-value bio-oil and biogas, iron-containing pyrolysis residues are further treated to obtain Fe3O4-BC serving as a magnetic flocculant, allelochemicals in an algae solution can be adsorbed and removed while microalgae flocculation is enhanced, and the inhibition effect of the culture solution on the microalgae during cyclic utilization is reduced. According to the invention, the culture, harvesting and energy utilization of microalgae can be synchronously realized, the problems of large water consumption in microalgae culture, difficult harvesting, incapability of effectively recycling a culture solution and the like are effectively solved, and a technical basis is provided for industrial application of energy microalgae.

ENCAPSULATION OF MICROALGAE IN HYDROGEL POLYMER AND HIGH-DENSITY ALGAL BIOMASS

Resumen de: WO2025166275A1

Aspects of the present disclosure generally relate to a new class of compositions that include microalgae encapsulated within a polymerized hydrogel. Aspects of the present disclosure also generally relate to high density algal biomass and to new processes for growing algal biomass. In an aspect is provided a composition that includes an encapsulant comprising a polymerized hydrogel, and microalgae encapsulated within the encapsulant. In another aspect, a process for encapsulating algae is provided. The process includes: introducing, to a device, a stream of core fluid comprising microalgae and a hydrogel precursor; contacting the stream of core fluid with two streams of a focusing fluid to form a thinner stream of core fluid comprising the microalgae and the hydrogel precursors, the focusing fluid having a different viscosity than the core fluid; and exposing the thinner stream of the core fluid to UV light to form a composition described herein.

NOVEL MICROALGAE AND USE FOR SAME

Resumen de: US2025250533A1

Provided is an alga belonging to Cyanidiophyceae having a diploid cell and a haploid cell form. Also provided is a nutrient composition containing an alga belonging to Cyanidiophyceae or an extract thereof.

Methods And Materials For Producing Recombinant Viruses In Eukaryotic Microalgae

Resumen de: AU2025203546A1

The present invention is directed to methods and materials for producing recombinant viruses. In particular, methods and materials are provided for producing recombinant viruses in eukaryotic microalgae such as Chlamydomonas reinhardtii. Recombinant adeno- associated viruses are examples of recombinant viruses produced according to the invention. The present invention is directed to methods and materials for producing recombinant viruses. In particular, methods and materials are provided for producing recombinant viruses in eukaryotic microalgae such as Chlamydomonas reinhardtii. Recombinant adeno- associated viruses are examples of recombinant viruses produced according to the invention. ay h e p r e s e n t i n v e n t i o n i s d i r e c t e d t o m e t h o d s a n d m a t e r i a l s f o r p r o d u c i n g r e c o m b i n a n t v i r u s e s n a y p a r t i c u l a r , m e t h o d s a n d m a t e r i a l s a r e p r o v i d e d f o r p r o d u c i n g r e c o m b i n a n t v i r u s e s i n e u k a r y o t i c m i c r o a l g a e s u c h a s h l a m y d o m o n a s r e i n h a r d t i i e c o m b i n a n t a d e n o - a s s o c i a t e d v i r u s e s a r e e x a m p l e s o f r e c o m b i n a n t v i r u s e s p r o d u c e d a c c o r d i n g t o t h e i n v e n t i o n

PHOTOBIOREACTOR AND METHOD FOR GROWING MICROALGAL-TYPE BIOLOGICAL ORGANISMS

Resumen de: WO2024068658A1

The invention relates to a photobioreactor (1) for growing microalgal-type biological organisms, the growth being based on autotrophy under light that is essentially, or even exclusively, artificial, wherein the organisms grow in a nutrient-rich liquid substrate circulating in the photobioreactor, the photobioreactor comprising at least one cell (3) referred to as an illuminated cell, the photobioreactor (1) being characterized in that the illuminated cell (3) comprises at least one spiral-shaped transparent pipe and in that the illuminated cell (3) comprises at least two sections referred to as lighting sections which are associated with the transparent pipe and arranged on either side of the transparent pipe.

A METHOD FOR PREPARING AND SELECTING A POLYMERIC FLOCCULANT FOR USE IN HARVESTING MICROALGAE

Resumen de: AU2023350703A1

The present invention relates to a method for harvesting microalgae, the method comprising (i) mixing one or more monomers, a photoinitiator, water and a deoxygenation system to form a mixture; (ii) exposing the mixture to UV light so as to form an aqueous solution of one or more polymers; (iii) introducing the aqueous solution of one or more polymers to a suspension comprising microalgae in an amount sufficient to produce flocculated microalgae; and (iv) harvesting the flocculated microalgae. The present invention also relates to a method for preparing and selecting a polymeric flocculant for use in harvesting microalgae and a related apparatus.

Functional unannotated protein CpsA and application thereof

Resumen de: CN120424182A

The invention discloses a function unannotated protein CpsA and application thereof, and belongs to the technical field of biochemistry. The CpsA protein provided by the invention is a thylakoid membrane protein which is coded by synpcc79421022 in synechococcus slender PCC 7942, and the function of the thylakoid membrane protein is not annotated. The protein can be specifically combined with an early PSII assembly factor Psb28, formation and stability of a PSII final oxygen evolution complex are promoted, and the assembly efficiency of a light system II and formation of a functional complex are remarkably improved. In addition, the CpsA protein is highly conserved in cyanobacteria, microalgae and land plants, and has wide application potential.

Indoor microalgae culture device

Resumen de: CN223189183U

The utility model discloses an indoor microalgae culture device, which belongs to the technical field of culture devices and comprises a box body for microalgae culture, the box body is of a square open structure, a light source structure is arranged at the top of the box body and comprises light wavelength adjusting buttons, a lamp tube and a fixer, and the light wavelength adjusting buttons are mounted at two ends of the lamp tube. The light wavelength adjusting button and the lamp tube are fixedly connected through a fixing device, and the lamp tube is fixed at the top end of the box body through the fixing device; the box body is provided with an aeration device, a temperature control device, a pH monitoring device and a water body circulating device, the growth conditions of microalgae can be accurately controlled and optimized, the wavelength of light generated by a lamp tube arranged on the device can be adjusted in a wide range, the optimal wavelength suitable for microalgae growth can be conveniently set, and the microalgae growth efficiency is improved. The design of the cuboid box body and the growth environment parameter adjusting system can realize water-saving culture of microalgae and promote formation of a microalgae biological membrane.

3 Carbon dioxide reduction device comprising three-dimensional porous hydrogel structures and microalgae and manufacturing method thereof

Resumen de: KR20250116963A

본 발명은 3차원 다공성 하이드로겔 구조체 및 미세조류를 포함하는 이산화탄소 저감 장치 및 이의 제조방법에 관한 것으로, 이산화탄소를 흡수하고 산소 및 유기물을 생산할 수 있는 미세조류를 3차원 다공성 하이드로겔 구조체에 포함시킬 경우, 미세조류가 공기 중의 이산화탄소; 및 태양광에 노출되는 표면적이 증가하여 미세조류의 이산화탄소 흡수 속도가 증가하는 것을 확인함으로써, 이산화탄소 저감 장치로써 유용하게 활용될 수 있다.

Floating type offshore comprehensive utilization platform

Nº publicación: CN120397179A 01/08/2025

Solicitante:

HUANENG YANTAI NEW ENERGY CO LTD
HUANENG CLEAN ENERGY RES INSTITUTE
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\u4E2D\u56FD\u534E\u80FD\u96C6\u56E2\u6E05\u6D01\u80FD\u6E90\u6280\u672F\u7814\u7A76\u9662\u6709\u9650\u516C\u53F8

Resumen de: CN120397179A

The invention provides a floating type offshore comprehensive utilization platform. The floating type offshore comprehensive utilization platform comprises a floating body platform, a buoy, a supporting unit, a photovoltaic module, a microalgae culture bin, a circulating water tank and a rough machining tank, a plurality of buoys extending below the sea level are arranged at the bottom of the floating body platform, and supporting units are arranged between the buoys and the floating body platform; the microalgae cultivation bin is arranged below the sea level, and the microalgae cultivation bin is fixed to the supporting unit; the circulating water tank and the rough machining tank are both arranged on the floating body platform, the first output end of the circulating water tank is connected with one end of the microalgae cultivation bin, and the first input end of the circulating water tank is connected with the other end of the microalgae cultivation bin through the rough machining tank. The method has the technical effects that the integration of offshore power generation and offshore high-value microalgae culture is realized, and the total income is increased; a marine photovoltaic floating body platform is fully utilized, a culture space and an equipment platform are provided for microalgae culture, and the photovoltaic module has the advantage of self-energy supply.