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Rapid campylobacter jejuni detection method and kit based on specific molecular target rpoZ

Resumen de: CN120290758A

The invention discloses a campylobacter jejuni rapid detection method and a kit based on a specific molecular target rpoZ. The sequence of the molecular target is as shown in SEQ ID NO. 1. The PCR/qPCR detection kit for detecting campylobacter jejuni is established on the basis of the target rpoZ, has extremely high specificity, only has specific amplification signals for nucleic acid of campylobacter jejuni, and has no cross reaction for other common food-borne pathogenic bacteria such as escherichia coli, salmonella and staphylococcus aureus. Meanwhile, the sensitivity is high, and the nucleic acid detection target can detect the campylobacter jejuni genome DNA as low as 132fg/mu L. The method can effectively detect campylobacter jejuni in samples such as food, is high in specificity, good in accuracy and simple to operate, and can be applied to the fields of food safety and the like.

Multiple qPCR (quantitative polymerase chain reaction) kit for detecting food-borne pathogenic bacteria of aquatic products and application of multiple qPCR kit

Resumen de: CN120290757A

The invention belongs to the technical field of biological detection, and discloses a multiple qPCR (quantitative polymerase chain reaction) kit for detecting food-borne pathogenic bacteria of aquatic products and application of the multiple qPCR kit. The kit comprises primers and a probe group for detecting vibrio parahaemolyticus, listeria monocytogenes and salmonella, and the sequences of the primers and the probe group are as shown in SEQ ID NO.1-9. According to the invention, probes with different fluorescence labels are utilized to realize simultaneous detection of three food-borne pathogenic bacteria of vibrio parahaemolyticus, listeria monocytogenes and salmonella in aquatic products. The method has the characteristics of short time consumption, good specificity, high sensitivity, high economic benefit and the like, has important significance on molecular diagnosis of the food-borne pathogenic bacteria in the aquatic products, and meets the detection requirements of simultaneously detecting the three food-borne pathogenic bacteria in the aquatic products.

Lactococcus formosanus strain with helicobacter pylori co-aggregation capability and application of lactococcus formosanus strain

Resumen de: CN120290363A

The invention discloses lactococcus formosanus with coaggregation capacity with helicobacter pylori and application of the lactococcus formosanus, and belongs to the technical field of microorganisms and the technical field of medicines. The lactococcus formosanus CCFM1461 screened by the invention has relatively strong gastric environment tolerance and acid production capacity, the copolymerization rate of the lactococcus formosanus CCFM1461 and helicobacter pylori within 120 minutes can reach 69.94%, the adhesion of the helicobacter pylori in AGS cells can be reduced, and the adhesion rate of the helicobacter pylori is reduced by 43.79%; the strain also has strong co-aggregation ability with other pathogenic bacteria (Escherichia coli, Staphylococcus aureus, Shigella, Salmonella typhimurium and Pseudomonas fluorescens), and has huge application prospects in the aspects of removal of helicobacter pylori, preparation of a helicobacter pylori adhesion scavenger and the like.

Marker and method for identifying germplasm of Salmonella salmon

Resumen de: CN120290742A

The invention discloses an identification marker and an identification method for germplasm of Salmonella salmon, and relates to an identification method for Salmonella salmon. According to the method, the problem of shortage of a Salmon salmon germplasm identification technology is solved. The method comprises the following steps: 1, extracting genome DNA of a to-be-identified sample; 2, carrying out PCR (Polymerase Chain Reaction) amplification by using a species specific primer; 3, agarose gel electrophoresis, recovery and purification; and 4, sequencing the purified PCR product, comparing the sequencing result with an identification sequence, splicing a homologous sequence, and comparing the difference of five basic groups positioned at the 15th bp, the 23rd bp, the 29th bp, the 39th bp and the 84th bp. The molecular marker is used for identifying the salmon arsalmonella, the salmon americana, the oncorhynchus masou and the oncorhynchus mykiss, has the characteristics of high efficiency, rapidness, stable result and high accuracy, and has important application value in the aspect of germplasm identification of the salmon arsalmonella.

BIOSENSORS USING CARBON NANOTUBES AND AN ELECTRONIC READER FOR USE WITH THE BIOSENSORS

Resumen de: US2025224367A1

Disclosed herein are biosensors, and, more particularly, biosensors using carbon nanotubes, an electronic reader for use with the biosensors, and systems and methods employing them. The biosensors employ preserved biologics on the carbon nanotubes which results in shelf-stable, robust biosensors.

Composition and Methods for Preventing and Treating African Swine Fever in Wild and Domestic Swine

Resumen de: US2025222101A1

A composition for treatment of African Swine Fever in swine includes avian-sourced antibodies specific for an African Swine Fever Virus isolate. These antibodies are produced by avian animals immunized against the African Swine Fever Virus isolate and mixed with a protective/reactive matrix obtained from, isolated from, or derived from, non-hyperimmune colostrum.

KLEBSIELLA VACCINE AND METHODS OF USE

Resumen de: US2025222089A1

Provided are compositions and methods that include a K. pneumoniae yidR protein or an antigenic segment of the protein, and homologous of the protein, and antigenic segments of the homologs. The compositions can be provided as vaccine formulations for use with humans and non-human animals, including but not limited to dairy cows. The compositions and methods are useful for prophylaxis and/or therapy of conditions associated with Gram negative bacteria that include K. pneumonia, E. coli, and other pathogenic Gram negative bacteria. The conditions include such bacterial infections generally, and include specifically mastitis and metritis. The compositions and methods can also improve fertility and milk production. Administration of the compositions can improve the likelihood of a first service conception.

一种CpG佐剂与抗原的生物共价结合方法

Resumen de: CN120267813A

本发明涉疫苗领域，具体而言涉及一种CpG佐剂与抗原定点共价结合方法。本发明方法采用分步偶联策略，使用HUH蛋白与相匹配的特异性识别ssDNA序列偶联系统、异肽键分子粘合剂系统以及融合表达系统等实现CpG佐剂与抗原以1:1的摩尔比定点偶联，并且所得到的偶联物具有提高疫苗的免疫刺激的效果。

Hermetia illucens breeding feed and preparation method thereof

Resumen de: CN120266989A

The invention discloses a hermetia illucens breeding feed and a preparation method thereof, and belongs to the technical field of feeds. The hermetia illucens breeding feed comprises a microbial preparation, kitchen garbage, natural substances, meal substances and modified shell powder. The modified shell powder is prepared from acid-treated shell powder. The invention also provides a preparation method of the composition. According to the application, the specially modified shell powder is synergistically used with the natural plant extract and the compound probiotics, so that synergism and unification of four targets of nutrition, antibiosis, deodorization and environmental stability are realized, and growth of pathogenic bacteria such as salmonella and escherichia coli is effectively inhibited; the problem of corruption caused by humidity and ventilation conditions of the breeding environment can be improved for a long time. The modified shell powder is used as a composite functional carrier, a probiotic attachment and stable release platform is provided by utilizing a porous structure of the modified shell powder, and the survival rate and the resource conversion efficiency of the hermetia illucens are systematically improved by combining with the continuous effect of natural active substances, so that the method has good innovativeness and practicability.

Bacillus safensis MLL-5 as well as complex microbial inoculant and application thereof

Resumen de: CN120272384A

The invention relates to the technical field of microorganisms, in particular to bacillus safensis MLL-5 and a complex microbial inoculant and application thereof.The strain MLL-5 is separated from rumen contents of healthy cattle by a research group, and it is detected that the strain has the good beta-1, 4-glucanase producing capacity and can be used for producing beta-1, 4-glucanase. The antibacterial effect is achieved on escherichia coli, yersinia enterocolitica, shigella flexneri and salmonella typhimurium; the bacillus safensis MLL-5 has relatively good fermentation capability on the cassava residues, can effectively reduce the cyanide content of the cassava residue fermented feed and improve the protein content of the cassava residue fermented feed, is a good biological fermentation agent aiming at the cassava residues, and has good application prospects. After Jersey cattle are fed with manioc waste fermented feed prepared from the strain and the complex microbial inoculant, it is found that addition of 10%-15% of fermented manioc waste into daily ration does not have adverse effects on the growth performance and health conditions of the cattle, the number of diarrhea times is obviously reduced, and the manioc waste fermented feed is safe.

Establishment method of salmonella pullorum challenge model

Resumen de: CN120267709A

The invention discloses a method for establishing a salmonella pullorum challenge model, and relates to the technical field of biological medicines. The invention discloses a method for establishing a salmonella pullorum challenge model. The method comprises the following steps: taking a 12-day-old chick embryo, and injecting a chitosan oligosaccharide solution into a chick embryo air chamber; placing the chick embryos injected with the chitosan oligosaccharide solution into an incubator for incubation until the chick embryos are 14 days old; salmonella pullorum is injected into an air chamber of the 14-day-old chick embryo for challenging; after challenging the chick embryos, continuously incubating the chick embryos in an incubator until 21 days old; and after the chick embryos are incubated to 21 days old, hatched and changed into chicks, establishing a toxic attack model. According to the invention, the method of the challenge model is creatively optimized, and the optimal chitosan solution injection concentration and the optimal challenge concentration of salmonella pullorum are screened; the salmonella pullorum challenge model is built through the method, the highest morbidity and survival rate of chicks can be controlled after 24 h, and it can be guaranteed that the morbidity time is uniform.

一种减毒鼠伤寒沙门氏菌及其应用

Resumen de: CN120249162A

本发明提供一种减毒鼠伤寒沙门氏菌及其应用，所提供的减毒鼠伤寒沙门氏菌，是通过敲除relA基因、manA基因和sifA基因制备的。本发明所提提供的三基因沙门氏菌减毒株，通过口服免疫将抗原呈递给宿主细胞，从而诱导机体产生免疫应答。沙门氏菌作为活载体的作用机理为携带细菌抗原通过肠道上皮后，通过肠道粘膜将其转运至免疫细胞，触发了宿主的免疫应答。本发明构建的减毒株经安全性检测发现，该减毒株安全性高，可作为载体，运载其他致病抗原或药物到达作用部位，进而起到载体作用。

Fluorescent colorimetric card based on fluorescent nanofibers and method for rapidly detecting hydrogen sulfide gas by using fluorescent colorimetric card

Resumen de: CN120253780A

The invention belongs to the technical field of rapid detection of food and agricultural products, and particularly relates to a fluorescent colorimetric card based on fluorescent nanofibers and a method for rapidly detecting hydrogen sulfide gas by using the fluorescent colorimetric card. The method comprises the following steps: firstly, synthesizing gold nanoclusters; then modifying a vinyl alcohol-ethylene copolymer nanofiber membrane based on the synthesized gold nanoclusters, and finally preparing the fluorescent colorimetric card based on the vinyl alcohol-ethylene copolymer nanofiber membrane modified by the synthesized gold nanoclusters. The fluorescent colorimetric card prepared by the invention can realize visual reading of hydrogen sulfide gas response signals, so as to realize qualitative identification of salmonella typhimurium in food. Moreover, the prepared fluorescent colorimetric card has the advantages of low cost, portability, visual detection and the like, is suitable for non-destructive detection of salmonella typhimurium in multiple scenes, and has important significance for guaranteeing the safety of food and agricultural products.

自己免疫疾患および炎症性疾患のための処置レジメン

Resumen de: CN119744171A

The present disclosure relates to novel therapeutic regimens for the treatment of autoimmune and other inflammatory diseases using BTK (Bruton's Tyrosine Kinase) inhibitors. In particular, the present disclosure relates to treatments that combine BTK inhibitor treatments with vaccination to avoid, reduce the risk of staining, or ameliorate infection. In addition, the present disclosure relates to vaccination in the presence of a BTK inhibitor.

RECOMBINANT SALMONELLA STRAIN AND USE THEREOF

Nº publicación: WO2025143588A1 03/07/2025

Solicitante:

INDUSTRY ACADEMIC COOPERATION FOUNDATION CHOSUN UNIV [KR]
\uC870\uC120\uB300\uD559\uAD50\uC0B0\uD559\uD611\uB825\uB2E8

Resumen de: WO2025143588A1

The present invention relates to a spheroplast protein y (Spy)-exogenous protein-secreting Salmonella strain, which possesses a Spy protein-coding gene and has the rpoE gene deleted. The recombinant Salmonella strain according to the present invention, while exhibiting attenuated virulence due to the Spy protein, can maintain structural stability when fused with exogenous proteins such as pathogenic viruses, therapeutic proteins, or antigenic epitopes, through the structure and chaperone functions of the Spy protein. Therefore, the present invention can serve as a live attenuated vaccine. Furthermore, the present invention can be used as a protein delivery vehicle, a drug delivery vehicle, and in antigen-antibody reaction-based techniques for applications in other areas of biotechnology.