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RPA-CRISPR/Cas12a-based magnetic control ratio fluorescent biosensor as well as preparation method and application thereof

Resumen de: CN122016744A

The invention provides a magnetic control ratio fluorescent biosensor based on RPA-CRISPR/Cas12a as well as a preparation method and application of the magnetic control ratio fluorescent biosensor, and belongs to the technical field of fluorescent biosensors and pathogenic bacterium detection. The magnetic control ratio fluorescence biosensor comprises a fluorescence reference signal element, a fluorescence response signal element and a specific recognition element, according to the magnetic control ratio fluorescent biosensor, high-sensitivity and specific detection of staphylococcus aureus and salmonella is realized through the magnetic and fluorescent characteristics of the fluorescent reference signal element, the fluorescent characteristic of the fluorescent response signal element and the nucleic acid cleavage activity of Cas12a; the magnetic control ratio fluorescent biosensor has a built-in calibration function, can reduce interference brought by the environment and instruments, has good selectivity to staphylococcus aureus and salmonella, has good anti-interference performance to other bacteria, and can be used for rapid detection in the field of food safety.

Lactobacillus plantarum strain LT400 and application thereof

Resumen de: CN122012354A

The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum strain LT400 and application thereof.The lactobacillus plantarum strain LT400 is separated from acid materials, can remarkably improve the aroma of sour mustard and increase the content of flavor substances, has a bacteriostatic effect on Escherichia coli, and can be used for preparing sour mustard. A fermentation product obtained by fermenting the strain and leaf mustard has an antibacterial effect on salmonella enteritidis, escherichia coli and staphylococcus aureus; the bacterial strain LT400 cooperates with alkaloids: mulberry alkaloid, passion fruit alkaloid and/or rosa roxburghii tratt fruit alkaloid, so that the brightness of the pickled Chinese cabbages can be effectively improved, and the pickled Chinese cabbages with better color and luster can be prepared; experiments prove that the alkaloid comes from fruits, the product source is green and healthy, the alkaloid is a healthy and effective pickled Chinese cabbage brightness improving agent, the alkaloid and the strain LT400 cooperate to provide a new thought for subsequent development of an anti-browning agent, the fermentation liquor has the value of researching and developing antibacterial peptides, and a new technical concept is provided for later improvement of the added value of the product.

Bacteriophage of lytic salmonella pullorum and application of bacteriophage

Resumen de: CN122012415A

The invention discloses a lytic salmonella pullorum bacteriophage and application thereof.The bacteriophage is provided with a polyhedral head and a contractile tail, the average diameter of the head is about 71 nm, the average diameter of the tail is about 126 nm, the bacteriophage belongs to the muscular tail bacteriophage family, and the bacteriophage can split 15 salmonella pullorum strains in 18 salmonella clinical isolates and can be used for treating salmonella pullorum. The incubation period is about 40 minutes, the cracking period is about 170 minutes, and the cracking amount reaches 142 PFU/cell. The lytic salmonella pullorum bacteriophage, the lytic salmonella pullorum bacteriophage and the separated bacteriophage have the advantages of wide host spectrum, high splitting efficiency, low optimal infection complex number, low production cost and the like, clinical tests show that the death rate of infected chickens can be remarkably reduced, the detection rate of salmonella in visceral organs is reduced, and the application prospect is wide. The salmonella pullorum prevention and control agent can effectively prevent and control salmonella pullorum, relieve pathological injuries, purify chicken flocks after long-term use, improve the laying rate and the laying quality, and provide a novel safe, efficient, economical and environment-friendly salmonella pullorum prevention and control scheme for poultry breeding industry.

一种基于SERS的快速超灵敏检测大肠杆菌O157:H7的方法

Resumen de: CN121995052A

0001 本发明属于生物检测技术领域，具体公开了一种基于SERS的快速超灵敏检测大肠杆菌O157:H7的方法，包括如下步骤：制备免疫磁珠捕获纳米粒子、制备贵金属或贵金属复合纳米粒子溶胶、制备免疫贵金属或贵金属复合信号纳米粒子、制备三明治式夹心结构、大肠杆菌O157:H7检测。本发明的方法以抗体作为特异性识别元件，同时特异性结合并测定大肠杆菌O157:H7，一方面能够稳定纳米材料在检测体系中的分散状态，另一方面能够高特异性和高亲和力地识别目标蛋白，并且稳定性好，制备成本低，在很大程度上提高了检测的准确性；本发明检测速度更快本发明检测流程极简，用时短，适配现场快检与批量筛查，同时检出限低，兼具超快检测速度与超高检测灵敏度，可精准识别痕量目标菌体。

FOOD POISONING BACTERIA DETECTION SENSOR

Resumen de: US20260117162A1

0000 The present disclosure relates to a food poisoning bacteria detection sensor. According to an embodiment, it is possible to provide the food poisoning bacteria detection sensor including: a substrate part; a liquid crystal part that is formed on the substrate part and is composed of a micro partition wall partitioning a unit pixel and a liquid crystal structure layer disposed inside the partitioned unit pixel; and an accommodating part that accommodates the substrate part and the liquid crystal part and includes an aqueous solution.

METHODS OF TREATMENT OF INTESTINAL INFECTIONS

Resumen de: US20260124215A1

The present invention relates to compositions and methods for treating or preventing infections, in particular infections by intracellular parasites such as Cryptosporidium spp. The present invention also relates to compositions and methods for treating or preventing viral or bacterial infections, in particular intestinal infections such as those caused by rotavirus and Salmonella spp. Infections. The methods comprise administration of, and the compositions comprise, a farnesyl-diphosphate farnesyltransferase 1 (FDFT1) inhibitor, such as lapaquistat.

Salmonella vectored therapies for treatment of cancer

Resumen de: US20260124255A1

0000 A genetically modified Salmonella cell (GMSC) engineered to display pattern-associated and danger-associated molecular patterns to recruit and enhance innate immunity and exhibit specific targeting to cells and regulated delayed lysis in vivo, the GMSC comprising a first heterologous nucleic acid that encodes a first gene product that causes the GMSC to be selectively localized to and/or internalized by a target cell in vivo and a second heterologous nucleic acid that encodes a second gene product that facilitates killing of the target cells following internalization.

REACTION MEDIUM AND METHOD FOR DETECTING SHIGA TOXIN-PRODUCING E.COLI AND/OR ENTEROHAEMORRHAGIC E.COLI

Resumen de: WO2025003117A1

The present invention relates to a gelled reaction medium for detecting, identifying, and/or isolating at least one Shiga toxin-producing strain of E. coli, the reaction medium comprising: - at least one toxin inducer, - at least one agglutinating conjugate comprising at least one specific binding partner of STX1 and/or at least one specific binding partner of STX2, coupled to a nanoparticle; - a concentration gradient of a compound for inhibiting non-target bacteria. The present invention also relates to the associated method for detecting and/or isolating Shiga toxin-producing E. coli which is likely to be present in a sample comprising enterobacteria.

METHOD FOR DETECTING AND CONFIRMING SHIGA TOXIN-PRODUCING ESCHERICHIA COLI AND/OR ENTEROHAEMORRHAGIC E. COLI

Resumen de: WO2025003119A1

The invention relates to a method for detecting and confirming at least one Shiga toxin-producing Escherichia Coli (STEC) which may be present in a sample comprising enterobacteria, comprising the following steps: - performing lysis of the sample, enabling lysis of the STECs in order to obtain a solution comprising the nucleic acids thereof; - bringing the solution of nucleic acids into contact with primers, making it possible to amplify at least the stx1 and/or stx2 gene or gene fragment; - if at least one of the stx1 and/or stx2 genes or gene fragments is amplified, part of the sample is deposited on an agar reaction medium comprising ■ at least one toxin inducer, ■ at least one agglutinating conjugate formed by at least one binding partner specific to the STX1 protein and/or at least one binding partner specific to the STX2 protein, which binding partner(s) is (are) coupled to a nanoparticle; - detecting and confirming the presence of at least one STEC by the appearance of a halo on the agar around the STEC.

用于多模式分泌新抗原的基因改造细菌

Resumen de: WO2025037284A2

A vaccine and methods of treatment thereof, wherein the vaccine comprises a recombinant Gram-negative bacteria genetically modified to express a first antigen fusion peptide comprising a neoantigen or series thereof, said neoantigen or series thereof associated with a first secretion signal from a double membrane-spanning secretion system and a second antigen fusion peptide comprising a homologous neoantigen or series thereof, associated with a second secretion signal from an outer membrane-spanning secretion system. The Gram-negative bacteria may be further modified for quadmodal transport. Specifically, the fusion peptides include signal peptides are each associated with a Type III (T3SS) and a Type V (T5SS) secretion system.

Method for producing water body environment protecting agent by fermenting hermetia illucens larva serous fluid

Resumen de: CN121974501A

The invention relates to a method for producing a water environment protective agent by fermenting hermetia illucens larva slurry, and belongs to the technical field of microbial fermentation. According to the present invention, the hermetia illucens larvae are adopted as the main raw material, the main raw material is ground into the slurry through the colloid mill, the small amount of the carbon source is supplemented, the Phaffia rhodozyma CGMCC NO.29217, the Bacillus coagulans CGMCC NO.5233 and the Clostridium butyricum CGMCC NO.4729 are subjected to the mixed bacteria synergistic fermentation, and the produced product can be widely used in the aquaculture and other application scenarios. Fermented hermetia illucens larva serous fluid is rich in astaxanthin, a fermented product has obvious acid fragrance and no peculiar smell, the total number of probiotics is larger than or equal to 5.0 * 10 < 8 > cfu/g, the pH value is smaller than 4.50, and the content of small molecule active polypeptide (acid soluble protein) of the fermented product is increased by 2.5-3 times compared with that before fermentation. Bacillus subtilis CGMCC (B) 63501 is used as indicator bacteria to detect that the content of bacteriostatic active substances after fermentation of hermetia illucens larva serous fluid is larger than 1.0 g/kg, pathogenic bacteria such as salmonella, vibrio parahaemolyticus and vibrio cholerae are not detected, and normal-temperature storage can be achieved. The preparat

Lactobacillus mucilaginosus RLF77 and application thereof

Resumen de: CN121975692A

The invention discloses a rabbit source fermentation lactobacillus mucus RLF77 and an application of the rabbit source fermentation lactobacillus mucus RLF77. The strain is separated from intestinal tracts of weaned young rabbits and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20252224. The bacterial strain has the excellent characteristics of being used as a probiotic, being resistant to gastrointestinal tract environment and high in intestinal epithelial cell adhesion rate, and being capable of effectively inhibiting various pathogenic bacteria such as escherichia coli, salmonella and the like. Animal experiments show that the production performance of weaned young rabbits can be remarkably improved, the diarrhea rate can be reduced, the survival rate and the oxidation resistance can be improved by feeding the rabbit-derived RLF77 strain, and the effect is superior to that of non-rabbit-derived probiotics. The invention provides a new strain resource for developing green breeding products for replacing antibiotics.

Primer group for simultaneously detecting four types of horse digestive tract bacteria and application of primer group

Resumen de: CN121975962A

The invention discloses a primer group for simultaneously detecting four equine digestive tract bacteria and application thereof, the primer group comprises primer sequences as shown in SEQ ID NO: 1-8, and the four equine digestive tract bacteria are salmonella enteritidis, salmonella typhimurium, clostridium difficile and lawsonia intracellular. Extracting total DNA (deoxyribonucleic acid) of a sample to be detected by using the excrement sample nucleic acid extraction kit; the total DNA is used as a template, and the primer group is used for multiple PCR reaction to obtain an amplification curve. According to the invention, a primer sequence with high sensitivity and specificity is adopted, so that the quality of a detection result is ensured; the detection method is simple to operate, time-saving and labor-saving; the detection flux is high, and the reagent consumable cost is low.

Cross-host multivalent bacteriophage, and preparation and application of bacteriostatic agent comprising bacteriophage

Resumen de: CN121975745A

The invention relates to a bacteriophage, in particular to a cross-host multivalent bacteriophage and preparation and application of a bacteriostatic agent of the bacteriophage, the multivalent bacteriophage is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2026045. The salmonella bacteriophage CCTCC NO: M 2026045 is found for the first time, is a multivalent bacteriophage, can cross-host infect and split multiple important livestock and poultry intestinal pathogens such as salmonella, escherichia coli, shigella, klebsiella pneumoniae and proteus mirabilis, solves the problem of narrow host range of a single bacteriophage, and improves the prevention and control efficiency. By inhibiting the overgrowth of the gram-negative pathogenic bacteria, the intestinal microecological balance can be regulated, so that the aim of effective prevention and control is fulfilled.

Nano-enzyme for preventing salmonella pullorum infection as well as preparation method and application of nano-enzyme

Resumen de: CN121971485A

The invention discloses a nano-enzyme for preventing salmonella pullorum infection, the nano-enzyme comprises kaempferol and cerium ions, the kaempferol and the cerium ions form a kaempferol-cerium composite nano-enzyme CeKae NPs through coordination, and the nano-enzyme has superoxide dismutase (SOD)-like activity and catalase (CAT)-like activity. The invention further discloses a preparation method and application of the nano-enzyme for preventing salmonella pullorum infection. The CeKae NPs nano-enzyme provided by the invention comprises kaempferol and cerium ions, has efficient SOD and CAT nano-enzyme activity, and can realize antioxidant self-cascade nano-enzyme reaction, effectively remove active oxygen and improve the pathological environment.

Salmonella bacteriophage nanoemulsion preparation as well as preparation method and application thereof

Resumen de: CN121970804A

The invention discloses a salmonella bacteriophage nano-emulsion preparation as well as a preparation method and application thereof. The salmonella bacteriophage nano-emulsion preparation is mainly prepared from the following active ingredients in percentage by mass: 5 to 15 percent of salmonella bacteriophage suspension, 84 to 94 percent of edible oil, 0.5 to 3.0 percent of Pickering stabilizer dispersion liquid and 0 to 2.0 percent of protective agent. The invention also discloses a preparation method of the salmonella bacteriophage nano-emulsion preparation and application of the salmonella bacteriophage nano-emulsion preparation in bacteriostasis and preservation of meat products. The salmonella bacteriophage nano-emulsion preparation disclosed by the invention is more essential in stable mechanism, more stable in system, more beneficial to bacteriophage activity maintenance, better in food applicability, safe, friendly, more durable in bacteriostatic action and more suitable for fresh keeping of meat products when being applied.

Method for analyzing influence of salmonella infection on chicken liver transcriptome RNA m6A modification

Resumen de: CN121983118A

The invention discloses a method for analyzing the influence of salmonella infection on chicken liver transcriptome RNA m6A modification, and relates to the technical field of bioengineering, and the method comprises the following steps: animal model establishment and sample collection, RNA extraction, sequencing and data quality control, bioinformatics analysis and differential m6A peaks identification, and data integration and function enrichment analysis. By integrating MeRIP-seq and a transcriptome sequencing technology, a dynamic regulation and control mechanism of RNA m6A modification in the process of infecting the chicken liver with salmonella is disclosed, an m6A modification and gene expression association analysis framework under an infection model is constructed, the analysis depth of an epigenetic regulation and control mechanism of poultry immune response is remarkably improved, and the application prospect is broad. And by establishing a standardized experimental process and a bioinformatics analysis system, accurate identification of differential modification sites and functional association thereof from a transcriptome level is realized, and a new technical path is provided for poultry disease resistance research.

METHODS AND SYSTEMS FOR THE RAPID DETECTION OF SALMONELLA USING INFECTIOUS AGENTS

Nº publicación: MX2025013800A 04/05/2026

Solicitante:

LABORATORY CORP OF AMERICA HOLDINGS [US]
LABORATORY CORPORATION OF AMERICA HOLDINGS

Resumen de: MX2025013800A

Disclosed herein are methods and systems for rapid detection of microorganisms in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as Salmonella-specific bacteriophage, allows detection of a specific microorganism, such as Salmonella spp. and an indicator signal may be amplified to optimize assay sensitivity.