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Primer probe composition and kit for simultaneously detecting 17 pathogenic microorganisms in food

Resumen de: CN119979776A

The invention discloses a primer probe composition and a kit for simultaneously detecting 17 pathogenic microorganisms in food. The primer probe composition is used for detecting salmonella, staphylococcus aureus, clostridium perfringens, escherichia coli O157, listeria monocytogenes, vibrio parahaemolyticus, bacillus cereus, vibrio cholerae, campylobacter jejuni, clostridium botulinum, proteusbacillus vulgaris, group A rotavirus, norovirus type GI, norovirus type GII and the like. The invention relates to a primer and probe combination for astrovirus, fiveleaf virus and enteric adenovirus. The primer and probe composition disclosed by the invention can be used for efficiently detecting 17 pathogenic microorganisms in food, has the advantages of simple and accurate judgment result, high sensitivity, strong specificity and high precision, and can meet the requirement of food detection industry on rapid detection.

Positively charged silicon shell modification-based magnetic fluorescent probe, preparation method and application

Resumen de: CN119979149A

The invention discloses a magnetic fluorescent probe based on positive silicon shell modification as well as a preparation method and application of the magnetic fluorescent probe. The probe adopts superparamagnetic Fe3O4 nanoparticles as an inner core and provides magnetic enrichment capacity; a cationic polymer electrostatically adsorbs multiple layers of quantum dot particles to serve as a fluorescent interlayer, and a fluorescent signal capable of being used for quantitative detection is provided; and a layer of aminated silicon dioxide shell with strong positive electricity is also arranged on the surface, so that the broad-spectrum capturing capability on bacteria with negative electricity is provided. By taking pseudomonas aeruginosa, streptococcus pneumoniae and salmonella typhimurium as examples, rapid enrichment and broad-spectrum quantitative analysis of the pseudomonas aeruginosa, streptococcus pneumoniae and salmonella typhimurium in a sample can be realized, a magnetic enrichment effect and multiple signal amplification of multiple layers of fluorescent quantum dots are realized, matrix interference in practical application is eliminated, and the detection sensitivity is high. The method has great potential in the aspects of high sensitivity and clinical monitoring of bacterial infection or pollution.

Lactobacillus rhamnosus FJS004 for inhibiting food-borne pathogenic bacteria as well as lactein and application of lactobacillus rhamnosus FJS004

Resumen de: CN119979385A

The invention relates to the technical field of probiotics, in particular to lactobacillus rhamnosus FJS004 for inhibiting food-borne pathogenic bacteria, lactein of lactobacillus rhamnosus FJS004 and application of lactobacillus rhamnosus FJS004. The lactobacillus rhamnosus FJS004 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 26, 2022, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.26253. The lactobacillus rhamnosus FJS004 can be used for preparing the lactobacillus rhamnosus FJS004. The lactobacillus rhamnosus FJS004 has the advantages that the lactobacillus rhamnosus FJS004 can be used for preparing the lactobacillus rhamnosus FJS004; the lactobacillus rhamnosus FJS004 and the lactein of the lactobacillus rhamnosus FJS004 provided by the invention have a good bacteriostatic or bactericidal effect on escherichia coli, salmonella, shigella, staphylococcus aureus, listeria monocytogenes, pseudomonas aeruginosa, bacillus cereus and enterobacter sakazakii.

Long-chain RNA Lnc012227 for inhibiting transmission of salmonella enteritidis ovary and application of long-chain RNA Lnc012227 in prevention and control of salmonella enteritidis infection

Resumen de: CN119979533A

The invention relates to the technical field of genetic engineering, in particular to a long-chain RNALnc012227 for inhibiting transmission of salmonella enteritidis ovary and application of the long-chain RNALnc012227 in prevention and control of salmonella enteritidis infection. The invention relates to a long-chain RNA (Ribonucleic Acid) Lnc012227 for inhibiting transmission of salmonella enteritidis ovary. The Lnc012227 provided by the invention can be used for indirectly regulating the expression of MAP3K8, activating NF-kappa B and MAPK signal channels and enhancing the inflammatory response of a host, so that S.Enteridis infection is controlled. The discovery not only provides a new understanding of molecular level for understanding the infection mechanism of salmonella in the duck body, but also provides a potential target spot for developing a new treatment strategy, and is helpful for reducing egg-laying duck ovarian infection and duck egg pollution caused by salmonella.

탄수화물-기반 항-살모넬라 백신의 개발

Resumen de: AU2023312737A1

Provided herein are vaccine composition comprising a

Antibacterial peptide Loong-3 and application thereof

Resumen de: CN119978065A

The invention belongs to the technical field of biology, and particularly relates to an antibacterial peptide Loong-3 and application thereof. The sequence of the antibacterial peptide Loong-3 is as follows: lysine, arginine, isoleucine, alanine, lysine, leucine, alanine, leucine, lysine, alanine, isoleucine, arginine, lysine, isoleucine, leucine, arginine, leucine and isoleucine. The antibacterial peptide has better antibacterial activity on gram-positive bacteria (staphylococcus aureus), gram-negative bacteria (escherichia coli and salmonella) and fungi (candida albicans), and has the characteristics of heat resistance, salt resistance, acid and alkali resistance, repeated freezing and thawing resistance, serum resistance and organic solvent resistance. Therefore, the antibacterial peptide Loong-3 has wide application prospects in the fields of preparation of drugs for resisting bacterial and/or fungal infection, animal husbandry development, prevention and treatment of human diseases, food preservation, detergents, cosmetics, antibacterial molecular materials and the like.

Primer probe, kit and detection method for simultaneously detecting 16 food-borne pathogenic bacteria

Nº publicación: CN119979740A 13/05/2025

Solicitante:

QINGDAO INT TRAVEL HEALTH CARE CENTER QINGDAO CUSTOMS PORT CLINIC
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Resumen de: CN119979740A

The invention discloses a kit for simultaneously detecting 16 food-borne pathogenic bacteria. Each group of probes is designed based on bacterial classification, virulence gene specificity or serotype marks of the pathogenic bacteria; a group A probe vibrio detection group is used for detecting vibrio cholerae universal type, vibrio cholerae O1 serotype, vibrio cholerae O139 serotype and vibrio parahaemolyticus; the B group of probe invasive enterobacteriaceae pathogen group is used for detecting escherichia coli O157: H7, shigella, salmonella and yersinia small intestine; the C group probe escherichia coli pathotype detection group is used for detecting enterotoxigenic escherichia coli ETEC, enteroinvasive escherichia coli EIEC, enteroaggregative adherent escherichia coli EAEC and pathogenic escherichia coli EPEC; and the D group of probes are used for detecting campylobacter jejuni, campylobacter colons, listeria monocytogenes and enterohemorrhagic escherichia coli EHEC in the campylobacter and toxin type pathogen group.