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85
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Última actualización
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Resultados 75 a 85 de 85
Resumen de: CN121108326A
The invention relates to the technical field of biological detection, and discloses a salmonella monoclonal antibody, a salmonella colloidal gold immunochromatography test strip based on the antibody and a preparation method of the salmonella colloidal gold immunochromatography test strip. Salmonella is used as an antigen, a polyclonal antibody and a monoclonal antibody are prepared respectively, the monoclonal antibody 8E71C8 is screened out, and the salmonella colloidal gold immunochromatography test strip is prepared. The purified monoclonal antibody is used as a 30 nm colloidal gold marker, the optimal pH and the optimal antibody labeling amount of binding of the monoclonal antibody and colloidal gold and the concentration of the polyclonal antibody used as a detection line are optimized, the prepared salmonella colloidal gold immunochromatography test strip is high in specificity, the sensitivity is about 1 * 10 < 4 > CFU/mL and is higher than the average detection lower limit of domestic similar products, and the salmonella colloidal gold immunochromatography test strip can be used for detecting salmonella. And the test strip is simple to operate and high in efficiency, can be used as a means for rapidly and visually detecting the salmonella, and has popularization and application values.
Resumen de: CN121109239A
The invention discloses an Arteratt lactobacillus TRM54999 strain with broad-spectrum antibacterial activity and application of the Arteratt lactobacillus TRM54999 strain, and belongs to the field of microorganisms. The strain is preserved in the China Center for Type Culture Collection (CCTCC) on June 4, 2024, and the preservation number is CCTCC NO: M 20241143. It is proved that the strain has good acid resistance and bile salt resistance and can grow in the environment with the bovine bile salt concentration being 1-2% (w/v) or in the pH value range being 3-7; the fermentation supernatant has an inhibition effect on nine pathogenic bacteria such as salmonella and the like, and the antibacterial effect is stable; meanwhile, experiments prove that the Agentlat lactobacillus TRM54999 has no wide drug resistance and can relieve the problems of aggravated drug resistance, damage to microbial ecological balance and the like caused by antibiotic abuse, and the strain provides a strain resource for preparing a biological bacteriostatic agent or an intestinal probiotic agent and has a wide application prospect.
Resumen de: CN121109656A
The invention belongs to the technical field of microbiological detection, and particularly relates to a method for rapidly detecting viable salmonella and application. The method comprises the following steps: mixing a sample to be detected with Felix O-1 bacteriophage, incubating at 37 DEG C to enable the Felix O-1 bacteriophage to infect and proliferate in the sample to be detected, and performing splitting decomposition treatment after infection proliferation is finished to obtain proliferated Felix O-1 bacteriophage DNA (Deoxyribose Nucleic Acid); felix O-1 bacteriophage DNA is used as a template for real-time fluorescence RPA amplification, and whether viable salmonella exists or not is judged through fluorescence signal intensity. According to the method provided by the invention, the defects that the traditional bacterial culture and biochemical confirmation method is long in time consumption and the original molecular biological method cannot conveniently distinguish dead and live are overcome. The method is short in detection time, the RPA reaction is carried out under a constant-temperature condition, equipment requirements are simple, and the method is suitable for field detection.
Resumen de: CN121109346A
The invention discloses an application of host-derived aFRK mRNA in preparation of drugs for resisting bacteremia caused by drug-resistant staphylococcus aureus. MRNA of mouse-derived FRK activated mutant protein aFRK is synthesized in vitro and is introduced into a host cell through a lipid nanoparticle (LNP) delivery system, and the host-derived aFRK mRNA can be used for preparing drugs for resisting bacteremia caused by drug-resistant staphylococcus aureus. The macrophage can obviously enhance the killing ability of the macrophage on intracellular drug-resistant staphylococcus aureus, listeria monocytogenes and salmonella, so that infectious bacteria are effectively removed, bacteremia symptoms are improved, the survival rate is increased, and the macrophage has important clinical application value.
Resumen de: CN121102169A
The invention belongs to the field of biological medicines, and particularly relates to a drug-loaded hybrid membrane system for targeting tumor-associated macrophages as well as a preparation method and application of the drug-loaded hybrid membrane system. The invention discloses a hybrid membrane carrier for targeting tumor-associated macrophages. The hybrid membrane carrier is formed by fusing attenuated salmonella typhimurium ghost and an M2pep peptide modified macrophage membrane. The invention also discloses a drug-loaded hybrid membrane system for targeting tumor-associated macrophages. The drug-loaded hybrid membrane system comprises a hybrid membrane carrier and a drug which is loaded in the hybrid membrane carrier and is used for immunoregulation/tumor resistance. The drug-loaded hybrid membrane system provided by the invention integrates the advantages of active targeting, homologous targeting, macrophage specific targeting, biocompatibility, immune activation efficiency and the like, and develops a new treatment mode for malignant tumors with low immunotherapy effect rate.
Resumen de: CN121109557A
The invention belongs to the technical field of food safety detection, and relates to a method for simultaneously extracting and detecting nucleic acid of listeria monocytogenes and salmonella. The method comprises the following steps: cracking listeria monocytogenes and salmonella by using a bacterial lysis solution and releasing nucleic acid of listeria monocytogenes and salmonella; the nucleic acid of the listeria monocytogenes and the nucleic acid of the salmonella are extracted at the same time through the synthesized magnetic beads Fe3O4 and Al < 3 + >, and the nucleic acid of the listeria monocytogenes and the nucleic acid of the salmonella are detected at the same time through double RPA/RT-RPA and RPA-LFA test paper. The method has the characteristics of rapidness, high sensitivity and low equipment requirement, and has practical application value in on-site instant detection of food safety.
Resumen de: CN121101008A
The invention discloses preparation of food processing by-product-based carbon dot fruit wax and application of the food processing by-product-based carbon dot fruit wax in food preservation, and belongs to the technical field of microorganisms. According to the invention, the citrus peel is used for preparing the carbon dots, the carbon dots are mixed with the Tween 80 and the palm wax to prepare the carbon dot fruit wax, the method is simple and feasible, the cost is low, and no extra chemical preservative is added. The carbon dot fruit wax prepared by using the method has a remarkable antibacterial effect on salmonella typhimurium and listeria monocytogenes, can maintain the weight, hardness, color and pulp quality of fruits during 14-day storage, and has good application value in the aspects of preservation of picked fruits and prolonging of the storage period.
Resumen de: WO2024197078A2
Provided herein are compositions and methods for treating benign nervous system tumors, including schwannomas, using attenuated mutants of Salmonella typhimurium comprising a spiC deletion, and, optionally, one or more checkpoint inhibitors.
Resumen de: US2025377362A1
The invention provides analytical methods for identifying and quantifying complex glycoconjugate compositions, in particular for the analysis of a glycoconjugate in a sample comprising at least 4 glycoconjugates.
Nº publicación: US2025375435A1 11/12/2025
Solicitante:
OHIO STATE INNOVATION FOUND [US]
Ohio State Innovation Foundation
Resumen de: US2025375435A1
Disclosed herein are compositions and methods for treating a Salmonella infection. Disclosed herein are compositions and methods for inhibiting FraB in Salmonella. Disclosed herein are methods of increasing 6-phospho-fructose-aspartate within Salmonella bacteria.
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