Alerta

Salmonella enterica ultra-fast detection kit based on whole-process nucleic acid detection chip and detection method of salmonella enterica ultra-fast detection kit

Resumen de: CN120843705A

The invention discloses a salmonella enterica ultra-rapid detection kit based on a whole-process nucleic acid detection chip and a detection method thereof. The kit comprises a rapid hot start DNA polymerase, a PCR reaction mixed solution, a positive reference substance and a negative reference substance, the kit also comprises the following primers which take the salmonella enterica V3-V4 region as a target sequence and are designed by a PCR fluorescent probe method based on a whole-process nucleic acid detection chip technical platform: an upstream primer F, a downstream primer R and a detection probe P, and the nucleotide sequences of the three primers are shown as SEQ ID NO. 1-3. According to the detection kit disclosed by the invention, a V3-V4 target sequence can be rapidly detected on the micro-fluidic chip within more than ten minutes, so that the accuracy and the specificity of detecting the salmonella enterica gene are ensured; the invention provides the salmonella enterica gene detection kit which is more comprehensive in detection effect, high in specificity, good in sensitivity, low in omission ratio, convenient, simple and easy to operate.

一种用于防治多种致病性肠杆菌感染的融合蛋白及应用

Resumen de: CN120842432A

本发明涉及一种应对多种致病性肠杆菌感染防治的融合蛋白、免疫原性组合物、重组简并疫苗，以及分子架构设计和应用等。本发明筛选到3种细胞免疫抗原Tuf、DnaK、fusA，构建的3种抗原的融合蛋白分子可显著抑制多种肠杆菌感染引起的组织病变，具有良好的免疫原性，起到有效防治和免疫保护作用，广谱且高效地预防多种致病性肠杆菌感染，具有广阔的工业应用前景。

Salmonella pullorum SP1910, pullorum disease antigen, preparation method and application

Resumen de: CN120829857A

The invention discloses a novel salmonella pullorum SP1910 strain, a pullorum disease antigen prepared from the novel salmonella pullorum SP1910 strain and a preparation method and application of the antigen, the strain has better stability and specificity, the detection effect of the antigen prepared from the strain on pullorum disease is superior to that of a commercial antigen, the pullorum disease can be efficiently detected, positive chickens are eliminated, and the detection cost is reduced. Chicken flocks are purified, infection of pullorum disease is reduced, and breeding benefits are improved.

- Recombinase polymerase amplification primer sets for detecting Salmonella and uses thereof

Resumen de: KR20250151120A

본 발명은 살모넬라(Salmonella) 검출을 위한 재조합효소-중합효소 증폭법(recombinase polymerase amplification)용 프라이머 세트 및 이를 이용한 핵산 측면 흐름 면역 분석(nucleic acid lateral flow immunoassay)용 살모넬라(Salmonella) 검출용 키트에 관한 것으로, 본 발명의 프라이머 세트는 살모넬라(Salmonella)속에 특이적인 유전자인 invA 유전자를 가장 높은 효율로 증폭하여 살모넬라(Salmonella) 검출이 우수할 뿐만 아니라, 재조합효소-중합효소 증폭(recombinase polymerase amplification) 수행시 비교적 낮은 온도에서 짧은 시간안에 증폭이 용의할 뿐만 아니라 상기 프라이머 세트의 역방향 프라이머 말단에 제1 표지물질이 결합하여 검출 가능한 발색입자인 금나노입자가 결합되고, 상기 프라이머 세트가 증폭하는 서열의 일부 서열에 특이적으로 결합하는 부위를 포함하는 프로브는 5' 말단에 제2 표지물질이 결합하여 핵산 측면 흐름 면역 분석(nucleic acid lateral flow immunoassay)에 적용시 RPA 증폭산물을 쉽게 검출 할 수 있는 살모넬라(Salmonella) 검출용 프라이머 세트 및 이의 용도를 제공한다.

Allogenic autophagy receptor NDP52 protein 202-site lysine acetylation antibody as well as preparation method and application thereof

Resumen de: CN120818041A

The invention relates to the field of biological medicine, in particular to an alloautophagy receptor NDP52 protein 202-site lysine acetylation antibody and a preparation method and application, the alloautophagy receptor NDP52 protein 202-site lysine acetylation antibody is based on NDP52 protein 202-site lysine acetylation polypeptide, and the invention provides the NDP52 protein 202-site lysine acetylation antibody and the preparation method and application. The preparation method of the antibody comprises the following steps: coupling the NDP52 protein 202-site lysine acetylated polypeptide with KLH and/or BSA to obtain an antigen, emulsifying the antigen, immunizing a rabbit to obtain immunized rabbit serum, and determining the antibody titer of the antibody in the immunized rabbit serum, and finally, purifying the antibody to obtain the protein 202 lysine acetylation antibody of the allogenic autophagy receptor protein NDP52. The prepared antibody can be applied to a salmonella bacterial infection detection reagent, and the detection accuracy is improved.

免疫原性组合物

Resumen de: WO2024192346A1

Technologies for providing immunogenic compositions (e.g., vaccines) and methods of inducing immune responses in subjects in need thereof.

Drug-resistant coliphage with efficient cross-species lysis capability and application thereof

Resumen de: CN120796203A

The invention discloses a drug-resistant Escherichia coli bacteriophage with efficient cross-species lysis capability and application of the drug-resistant Escherichia coli bacteriophage, the drug-resistant Escherichia coli bacteriophage is named as Escherichia coli bacteriophage PE-48, and the preservation number of the drug-resistant Escherichia coli bacteriophage is CCTCC (China Center for Type Culture Collection) NO: M 2025261. The strain not only can efficiently split pathogenic escherichia coli, but also can split salmonella, has efficient cross-species splitting capacity, and can effectively prevent and treat various symptoms caused by escherichia coli and relieve phenomena such as large-scale death and the like. The bacteriophage is separated from the natural environment, is a natural biological purification factor, and has the advantages of being green, environmentally friendly, safe, free of residues, efficient in bacteriostasis and the like. Escherichia coli and salmonella infection can be effectively prevented and treated in the chick breeding process.

Composite probe for detecting salmonella enteritidis and preparation method thereof

Resumen de: CN120796521A

The invention belongs to the technical field of microbiological detection, and discloses a composite probe for detecting salmonella enteritidis and a preparation method. The composite probe for detecting the salmonella enteritidis comprises magnetic nanoparticles FeO, wherein the surface of the magnetic nanoparticles FeO is modified with an aptamer apt which is specifically combined with the salmonella enteritidis; the surface of the long afterglow nano particle PLNPs is modified with a DNA (Deoxyribose Nucleic Acid) sequence cDNA (Complementary Deoxyribonucleic Acid) complementary with the aptamer; the aptamer apt and the complementary DNA sequence cDNA are subjected to hybridization to form a composite probe structure FeO-SEapt (at) PLNPs-cDNA (complementary deoxyribonucleic acid). The long afterglow luminescence characteristic of PLNPs (ZnGaO: Cr) is utilized, and an excitation light source is stopped before detection, so that signal acquisition completely avoids autofluorescence of a sample matrix, background fluorescence interference is thoroughly eliminated, and the signal-to-noise ratio is remarkably improved.

Recombinant bacillus subtilis capable of increasing pH of ruminant rumen fluid and producing compound cellulase and application thereof

Resumen de: CN120796130A

The invention relates to the technical field of microorganisms. The invention provides recombinant bacillus subtilis capable of increasing the pH value of ruminant rumen fluid and producing compound cellulase and application of the recombinant bacillus subtilis. The Latin name of the recombinant bacillus subtilis is bacillus subtilis, the preservation name is bacillus subtilis M4K2C, the preservation place is China Center for Type Culture Collection, the preservation date is August 14, 2024, and the preservation number is CCTCC NO: M20241786. When 4-5-month-old Shaanbei white cashmere goats are fed with the recombinant bacterium B.subtilis M4K2C provided by the invention for 60 days, the pH value of rumen fluid can be effectively increased. Recombinant bacteria B.subtilis M4K2C are fed to 4-5-month-old Shaanbei white cashmere goats, and relative abundance of harmful microorganism salmonella and Bild virus can be effectively inhibited by feeding the 4-5-month-old Shaanbei white cashmere goats for 30 days.

Combined medicine for treating colitis and application thereof

Resumen de: CN120789140A

The invention relates to the technical field of biological pharmacy, in particular to a combined medicine for treating colitis and application of the combined medicine, and in the combined medicine, the volume ratio of coptis chinensis exosomes to salmonella outer membrane vesicles is (1-2): (1-3); the concentration of the coptis chinensis exosome and the concentration of the salmonella outer membrane vesicles are both 9 mg/mL to 11 mg/mL. According to the application, the coptis chinensis exosome and the salmonella outer membrane vesicle are creatively combined for use, so that the limitation of a single treatment strategy is overcome, the treatment effect is remarkably improved, and the problem of side effects of an existing medicine is avoided. The traditional Chinese medicine composition is low in treatment cost, safer and free of drug resistance, and a more effective, safer and economical treatment method can be provided for inflammatory diseases such as colitis.

Preparation method of high-content cefpodoxime proxetil nanoemulsion

Resumen de: CN120788993A

The invention relates to the technical field of pharmaceutical preparations, in particular to a preparation method of a high-content cefpodoxime proxetil nanoemulsion with remarkable in-vitro antibacterial effect difference of avian salmonella and pathogenic escherichia coli. The nanoemulsion is prepared from the following raw materials in percentage by weight: 0.1%-1.5% of cefpodoxime proxetil; the oil phase is 1%-12% of origanum oil; the surfactant is prepared from 18%-25% of polyoxyethylated castor oil 40; the cosurfactant is 0.4%-5% of 1, 2-propylene glycol; and the balance of water. The advantages of the nanoemulsion and the cefpodoxime proxetil are integrated, a preliminary prescription is screened out by adopting a traditional nanoemulsion preparation method, and an optimal nanoemulsion prescription is obtained by designing and testing by adopting an L9 (34) multi-index orthogonal test method. The cefpodoxime proxetil injection has the remarkable effects that the solubility of the cefpodoxime proxetil is remarkably improved, the minimum inhibitory concentration and the minimum bactericidal concentration of the cefpodoxime proxetil to avian salmonella and pathogenic escherichia coli are remarkably reduced by using the origanum oil as the oil phase, and a pharmaceutical foundation is laid for improving the bioavailability of the cefpodoxime proxetil.

Preparation for treating chicken salmonellosis and preparation method thereof

Nº publicación: CN120789145A 17/10/2025

Solicitante:

GUANGXI AGRICULTURAL VOCATIONAL AND TECHNICAL UNIV
\u5E7F\u897F\u519C\u4E1A\u804C\u4E1A\u6280\u672F\u5927\u5B66

Resumen de: CN120789145A

The invention discloses a preparation for treating chicken salmonellosis. The preparation is prepared from the following raw materials in parts by weight: main materials: coptis chinensis extract, sanguisorba officinalis extract, Chinese knotweed herb extract, myrobalan, pomegranate rind and allicin; coating materials: cereal prolamin, a slow-release agent and a reinforcing agent; the auxiliary materials comprise an adhesive, an antioxidant and a phagostimulant. The preparation method comprises the following steps: S1, pretreating; S2, mixing; S3, granulating; S4, coating; the traditional Chinese medicine composition can effectively treat pullorum disease caused by chicken salmonellosis, is good in treatment effect, and improves breeding benefits; through a coating material, an acid-resistant layer with high mechanical strength and a sustained-release layer are formed, effective components are prevented from being decomposed and inactivated in advance, the components are prevented from being mechanically damaged, meanwhile, sustained release can be achieved, the effective components are prevented from being rapidly discharged, and therefore the treatment effect is further improved.