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Escherichia coli bacteriophage and expression and application of endolysin thereof

Resumen de: CN120555369A

The invention discloses expression and application of an Escherichia coli phage and endolysin thereof, and belongs to the technical field of microorganisms, the Escherichia coli phage VB-EcoM-WM is separated from diarrhea cattle manure of a certain cattle farm in Sichuan, the Escherichia coli phage VB-EcoM-WM is preserved in China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 2025810, the endolysin of the Escherichia coli phage VB-EcoM-WM has broad-spectrum lysing property on bacteria, and the endolysin of the Escherichia coli phage VB-EcoM-WM has broad-spectrum lysing property on bacteria. The endolysin is subjected to hydrophobic modification and prokaryotic expression to obtain the fused endolysin protein, so that the cleavage property of the endolysin is more broad-spectrum and efficient. The gene engineering modified fusion protein not only can crack host escherichia coli, but also has a bacteriostatic effect on 15 tested bacteria such as salmonella, streptococcus, staphylococcus and pasteurella. The minimum inhibitory concentration on escherichia coli is 0.1 mu g/ml. The traditional antibiotics can be replaced.

Microbial treatment flora for treating odor of household garbage treatment station

Resumen de: CN120555263A

The invention discloses a microbiological treatment flora for treating odor of a household garbage treatment station. Comprising the following microbial treatment flora components: 12 to 33 percent of biphasic photosynthetic bacteria, 0.2 to 1.5 percent of clostridium butyricum, 8 to 22 percent of bifidobacterium, 0.2 to 1.5 percent of nitrogen-fixing bacteria, 9 to 18 percent of mesophilic actinomycetes, 0.3 to 2 percent of potassium bacteria, 13 to 23 percent of subdigestion bacteria, 0.3 to 2 percent of phosphate solubilizing bacteria, 18 to 24 percent of bacillus megatherium, 0.5 to 3 percent of lactic acid bacteria and 0.5 to 3 percent of saccharomycetes. Fatty acids and sulfides are decomposed through the biphase photosynthetic bacteria through photosynthesis, stink precursor substances are reduced from the source, bifidobacteria and lactic acid bacteria reduce the pH of the environment through acid production, growth of stink-producing putrefying bacteria such as escherichia coli and salmonella is inhibited, release of stink gases such as ammonia and methyl mercaptan is reduced, saccharomycetes generate alcohol through saccharides, and the alcohol content is reduced. The removal rate of hydrogen sulfide and ammonia is greater than or equal to 80%, and the degradation rate of the volatile fatty acid is greater than or equal to 70%.

Lactobacillus plantarum RT1 with anti-inflammatory function and application thereof

Resumen de: CN120555287A

The invention discloses a lactobacillus plantarum RT1 with an anti-inflammatory effect and application of the lactobacillus plantarum RT1. The lactobacillus plantarum RT1 with the anti-inflammatory effect is screened by utilizing a J774-Dual macrophage inflammation model, the influence of the RT1 on the cell activity is evaluated, and the anti-inflammatory effect of the lactobacillus plantarum RT1 is researched. It is found that the lactobacillus plantarum RT1 significantly inhibits LPS and salmonella induced NF-kappa B activation, inhibits expression of pro-inflammatory cytokines IL-1beta, TNF-alpha and IL-6, meanwhile up-regulates the expression level of an anti-inflammatory cytokine IL-10, and maintains good cell activity; dSS-induced weight loss, occult blood, colon shortening and DAI score are significantly relieved, the MPO level and inflammatory cytokine expression are reduced, and colon tissue pathological damage is improved. The lactobacillus plantarum RT1 has the function of inhibiting intestinal inflammation, so that a new material is provided for the development of novel efficient anti-inflammatory probiotics.

Biological bacteriostatic agent and preparation method thereof

Resumen de: CN120536277A

The invention discloses a biological bacteriostatic agent and a preparation method thereof, and the preparation method comprises the following steps: step one to step three, inoculating lactic acid bacteria into a liquid fermentation culture medium, and carrying out three-stage fermentation culture; step 4, after the third-stage seed fermentation is finished, performing high-speed centrifugation on the fermentation liquid to obtain a bacterial sludge compound; 5, uniformly mixing the bacterial sludge compound with a protective agent, and performing freeze drying to obtain microbial freeze-dried powder; and 6, uniformly mixing the microbial freeze-dried powder, the auxiliary agent and the auxiliary factor to obtain the biological bacteriostatic agent. The biological bacteriostatic agent prepared by the method disclosed by the invention has remarkable bacteriostatic and bactericidal effects on various pathogenic bacteria, such as escherichia coli, salmonella and staphylococcus aureus; and main antibacterial substances come from lactic acid bacteria and metabolites thereof, so that pathogenic bacteria do not generate drug resistance to the antibacterial substances. The natural components are adopted for fermentation preparation, no chemical component is contained, the environment-friendly effect is achieved, and safety is high; and the preparation method is simple and easy for industrial production.

Lateral flow chromatography kit for high-sensitivity quantitative detection of salmonella enteritidis

Nº publicación: CN120539398A 26/08/2025

Solicitante:

QILU UNIV OF TECHNOLOGY SHANDONG ACADEMY OF SCIENCES
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Resumen de: CN120539398A

The invention belongs to the technical field of food microorganism detection, and provides a lateral flow chromatography kit for high-sensitivity quantitative detection of salmonella enteritidis, which comprises a salmonella enteritidis RPA primer group and a lateral flow chromatography test strip, raman label streptavidin, a Digoxin antibody and a streptavidin antibody are coated on the lateral flow chromatography test strip. The reporter probe is combined on the release pad of the flow measurement chromatography test strip, has two characteristics of colorimetry and SERS (Surface Enhanced Raman Scattering), can finish simultaneous qualitative and quantitative detection of pathogenic bacteria, and improves the detection sensitivity; and the Digoxin antibody and the streptavidin antibody are respectively combined on the detection line and the quality control line. The kit does not need to use large experimental equipment in the whole detection process, is high in detection accuracy and good in specificity, and is very suitable for real-time detection under the condition of relatively low development level.