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Preparation method of ratio type electrochemical luminescence biosensor, sensor and application of sensor in detection of escherichia coli O157: H7

Resumen de: CN121955376A

The invention discloses a preparation method of a ratio type electrochemical luminescence biosensor, the sensor and application of the sensor in detection of escherichia coli O157: H7, and relates to the technical field of nano materials and biological detection. The sensor provided by the invention comprises an Aptamer-Templet double-strand body, a phi29 polymerase, a Nb.BbvCI nicking enzyme, a probe 1 and a probe 2, wherein the probe 1 and the probe 2 are connected with each other; the method comprises the following steps: specifically recognizing surface protein of escherichia coli O157: H7, releasing a Templet chain, releasing a Trigger chain and opening a hairpin structure of a probe 1 under the assistance of polymerase and cutting enzyme, and opening a hairpin structure of a probe 2 by the opened probe 1, so that self-assembly of the probe 1 and the probe 2 is realized, and a detectable object is obtained; the detection limit of the sensor to escherichia coli O157: H7 is as low as 1 CFU/mL, and the sensor is wide in linear range, high in stability, suitable for multiple samples, high in specificity and easy and convenient to use and operate.

Primer probe combination, kit, nucleic acid detection method and application

Resumen de: CN121931266A

The invention provides a primer probe combination, a kit, a nucleic acid detection method and application, and relates to the technical field of biochemical detection. The primer probe combination comprises a first primer pair and a first probe, wherein the first primer pair and the first probe are used for specifically amplifying pseudomonas aeruginosa; the second primer pair and the second probe are used for specifically amplifying enterococcus; the third primer pair and the third probe are used for specifically amplifying staphylococcus aureus; the fourth primer pair and the fourth probe are used for specifically amplifying the salmonella enterica. According to the primer and probe combination, a specific nucleotide sequence is utilized, efficient and accurate recognition of pseudomonas aeruginosa, enterococcus, staphylococcus aureus and salmonella enterococcus is achieved in a complex intestinal environment, and non-specific interference is effectively avoided; meanwhile, synchronous quantitative detection of four pathogens is supported, the detection efficiency is remarkably improved, and an accurate and sensitive detection means is provided for rapid clinical evaluation of flora conditions.

Micro-fluidic chip for simultaneously detecting multiple food-borne pathogenic bacteria as well as preparation method and application of micro-fluidic chip

Resumen de: CN121927707A

The invention relates to a micro-fluidic chip capable of simultaneously detecting multiple food-borne pathogenic bacteria as well as a preparation method and application of the micro-fluidic chip, in particular to detection of the food-borne pathogenic bacteria, and belongs to the technical field of analysis and detection. The device comprises a functionalized glass slide, a PDMS chip layer and a copper net, the functionalized glass slide is located at the bottommost layer, and a PDMS chip layer in plasma bonding connection is arranged on the functionalized glass slide; a plurality of through holes are formed in the PDMS chip layer, and a copper net is placed in each through hole; a plurality of round holes with the same diameter are formed in the copper net. The micro-fluidic chip is prepared through preparation of a functionalized glass slide, preparation of a PDMS chip layer, preparation of a detection unit and training of a deep learning model and is used for detecting various food-borne pathogenic bacteria at the same time. The liquid crystal biosensor provided by the invention has the advantages of simplicity, portability, rapidness, sensitivity, low cost, no mark, visualization and the like. The method has a good practical application prospect in the fields of rapid detection and the like.

Application of electrochemical active bacteria sensitive to organochlorine pollutants in detection of biotoxicity of water quality

Resumen de: CN121917621A

The invention discloses application of electrochemical active bacteria sensitive to organochlorine pollutants in detection of water quality biotoxicity, and belongs to the technical field of water quality biotoxicity detection.After a bacteria solution of the electrochemical active bacteria is exposed in toxic pollutants, the water quality biotoxicity is determined according to an electric signal inhibition rate; the toxic pollutants are organic chlorine pollutants; the electrochemical active bacterium is a pseudomonas genus, and specifically, the pseudomonas genus is Pseudomonas lopnurensis or Pseudomonas delhiensis. The invention further discloses a preparation method of the electrochemical active bacterium. The method provided by the invention is expected to improve the sensitivity of determining the biotoxicity of the organic chlorine pollutants, overcome the application bottleneck of low sensitivity, and provide technical support for biotoxicity monitoring work.

Primer and method for detecting salmonella and drug-resistant gene thereof and application of primer and method

Resumen de: CN121915177A

The invention belongs to the technical field of microbiological detection, and particularly relates to a primer and a method for detecting salmonella and a drug-resistant gene thereof and application of the primer and the method. The gene of the salmonella is invA, and the drug resistance genes are blaCTX-M, blaNDM-1, blaTEM-1 and blaOXA; the gene of the salmonella is invA, and the drug resistance genes of the salmonella are blaCTX-M, blaNDM-1, blaTEM-1 and blaOXA; the primers comprise inner primers and outer primers of invA, blaCTX-M, blaNDM-1, blaTEM-1 and blaOXA, a primer group is designed according to conserved regions of a salmonella invA gene and part of beta-lactam drug-resistant genes blaNDM-1, blaTEM-1, blaOXA and blaCTX-M, a detection method of SYBR-LAMP and HNB-LAMP technologies is established, and screening of part of beta-lactam drug-resistant genes carried by animal-derived pathogenic strains is realized.

Electrochemical sensor for detecting escherichia coli in food and preparation method of electrochemical sensor

Resumen de: CN121917619A

The invention belongs to the technical field of harmful microorganism detection, and particularly provides an electrochemical sensor for detecting escherichia coli (escherichia coli O157: H7) in food and a preparation method of the electrochemical sensor. According to the electrochemical sensor, the surface of a substrate electrode is sequentially modified with an electron transduction layer and a recognition molecular layer; wherein the electron transduction layer is formed by an infinite coordination polymer composite material of gold nanoparticles; the recognition molecular layer is a nucleic acid aptamer. The electrochemical sensor is high in sensitivity, good in selectivity and low in detection limit; compared with other detection methods, the method does not need incubation and can be directly used for in-situ and rapid detection of bacteria.

Aquatic product quality safety detection device for rapidly screening food-borne pathogenic bacteria

Resumen de: CN121899348A

The invention relates to the technical field of aquatic product quality safety detection, in particular to an aquatic product quality safety detection device for rapidly screening food-borne pathogenic bacteria. Comprising a workbench, a support, a suction head bin, a sample frame, a detection module, a suction head recovery box, a movement module and a pipetting module, the support is fixedly arranged at the top of the workbench, and the suction head bin, the sample frame, the detection module and the suction head recovery box are arranged between the workbench and the support and are sequentially arranged from left to right; the movement module is fixedly arranged at the inner top of the bracket, and the pipetting module is movably arranged below the movement module; an integrated, processized and automatic detection platform is constructed, the complete process from suction head installation, sample/reagent suction, liquid transfer and distribution to final suction head discarding is automatically completed, instability and fatigability of manual operation are avoided, and the overall detection efficiency and consistency are remarkably improved.

Semi-quantitative detection kit for clostridium perfringens, detection method and application

Resumen de: CN121896313A

The invention belongs to the technical field of microbiological detection, and particularly provides a clostridium perfringens semi-quantitative detection kit, a detection method and application, the clostridium perfringens semi-quantitative detection kit comprises a liquid reagent A and a solid reagent B; the liquid reagent A comprises an aqueous solution in which tryptone, soy peptone, yeast extract powder and ammonium ferric citrate are dissolved; the solid reagent B comprises sodium pyrosulfite. According to the clostridium perfringens semi-quantitative detection kit provided by the invention, the specific liquid culture medium with an optimized formula is adopted, and the reagent A and the reagent B adopt a liquid-solid split charging design, so that sodium pyrosulfite is prevented from being degraded in a liquid environment, and the shelf life is prolonged. And a standard colorimetric card and a time comparison table can be prepared, so that subjective errors are reduced. In addition, the device can be integrated with a disposable sampling tool, a portable constant-temperature water bath cup and a timing prompt assembly to form a complete detection system which can be used after being opened, and the single detection cost is low.

Three-dimensional fluorescence detection method and system for identifying escherichia coli in bile

Resumen de: CN121899099A

The invention relates to the technical field of visible light absorption spectrum detection, in particular to a three-dimensional fluorescence detection method and system for identifying escherichia coli in bile, and the method comprises the following steps: generating a light source through a light source generation module; adjusting the intensity of the light source by using a light splitting module, and dividing the light source into two paths of light so as to obtain sample detection light and a reference spectrum; acquiring a background spectrum and a bile detection spectrum by utilizing a sample detection module based on the sample detection light; according to the reference spectrum, the background spectrum and the bile detection spectrum, the upper computer uses a spectrum detection model to identify escherichia coli in the bile sample. Based on the detection principle of three-dimensional fluorescence, the intelligent degree and efficiency of detection of escherichia coli in bile can be improved, and the detection cost can be reduced.

SERS method for high-sensitivity detection of aquatic product pathogenic bacteria

Resumen de: CN121899103A

According to the scheme, target bacteria (such as vibrio parahaemolyticus) are subjected to high-sensitivity SERS detection by utilizing a sandwich structure principle, Au (at) Ag-MPBA/PEG-Ab1 is prepared as an SERS probe for quantitative detection of the target bacteria, and enrichment of the target bacteria is effectively realized by combining a magnetic composite nano material Fe3O4 (at) TA/Ag NPs-Ab2, so that the SERS detection of the pathogenic bacteria in the aquatic products is realized. A sandwich structure detection system with high sensitivity and specificity to vibrio parahaemolyticus is formed, finally, signal amplification is achieved by preparing a two-dimensional flexible functional regenerated SERS substrate of a high-density SERS'hot spot 'with a bamboo cellulose membrane as a raw material, so that enrichment and sensitivity detection of vibrio parahaemolyticus is rapidly completed, the detection range is 10-107 CFU/mL, and the detection sensitivity is high. The method has great potential in the aspects of food safety monitoring and infectious disease diagnosis.

Constant-temperature rapid detection method for multiple nucleic acids of diarrheogenic escherichia coli

Resumen de: CN121874375A

The invention discloses a constant-temperature rapid detection method for multiple nucleic acids of diarrheogenic Escherichia coli, and belongs to the technical field of rapid detection of microorganisms. A primer probe group is designed according to specific virulence genes of diarrheogenic Escherichia coli, and a quadruple nucleic acid constant-temperature rapid detection system aiming at escV, bfpB, aggR and invE genes, a triple nucleic acid constant-temperature rapid detection system aiming at stx1, stx2 and pic genes and a triple nucleic acid constant-temperature rapid detection system aiming at It, sth and astA genes are established; the kit can be used for detecting diarrheagenic Escherichia coli EPEC, EHEC, ETEC, EAEC and EIEC, and the purpose of detecting the diarrheagenic Escherichia coli rapidly and conveniently is achieved. The technology consumes less time, can react at normal temperature, reduces dependence on professional instruments, can be applied to daily laboratory detection to shorten time, can also be applied to field on-site rapid diagnosis, and provides a new technical means for detection of diarrheogenic escherichia coli.

Escherichia coli mutant strain with high yield of L-cysteine and application of escherichia coli mutant strain

Resumen de: CN121852296A

The invention provides an Escherichia coli mutant strain with high yield of L-cysteine and application, and belongs to the technical field of bioengineering, the strain is named as MFAPA45QT, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO: 31970. According to the method, molecular modification means such as metabolic engineering and the like are combined with a traditional breeding method, and through mutation sites existing in 3-phosphoglycerate dehydrogenase and serine acetyltransferase, feedback regulation on key enzymes in the L-cysteine synthesis process is effectively relieved, and the yield of L-cysteine is increased; the escherichia coli mutant strain MFAPA45QT is obtained by carrying out ARTP mutagenesis coupling L-cysteine specific response biosensor high-throughput screening, the L-cysteine yield in a fermentation tank level can reach 31.11 g/L, and the method is suitable for industrial production of L-cysteine.

Method for detecting Escherichia coli in food by combining Fe3O4 (at) Mo-CDs nano-enzyme with aptamer

Resumen de: CN121831141A

The invention discloses a method for detecting Escherichia coli in food by combining Fe3O4 (at) Mo-CDs nano-enzyme with an aptamer, according to the method, near-infrared light response molybdenum-doped carbon dot modified Fe3O4 (at) Mo-CDs nano-enzyme is synthesized through a hydrothermal method, the Fe3O4 (at) Mo-CDs nano-enzyme has excellent NIR enhanced peroxidase activity and magnetism, 3, 3 ', 5, 5'-tetramethyl benzidine is oxidized to generate blue oxidized TMB, and the activity and magnetism of the NIR enhanced peroxidase are improved. After the surface of the Fe3O4 (at) Mo-CDs nano-enzyme is functionalized with an escherichia coli nucleic acid aptamer, the POD-like activity of the Fe3O4 (at) Mo-CDs nano-enzyme is inhibited, when Escherichia coli exists, the Fe3O4 (at) Mo-CDs nano-enzyme can be selectively combined with E.coli, the POD-like activity of the nano-enzyme is further inhibited, when the Fe3O4 (at) Mo-CDs nano-enzyme is used for detecting the E.coli in samples such as fruit juice and cakes, the detection limit is as low as 0.767 CFU/mL, and the Fe3O4 (at) Mo-CDs nano-enzyme has relatively high reliability and accuracy and can be widely applied to detection of E.coli. According to the invention, magnetic targeting separation and visual detection of E.coli are realized.

Primer group and kit for detecting avian pathogenic escherichia coli based on LAMP (Loop-Mediated Isothermal Amplification) method

Resumen de: CN121826198A

The invention discloses a primer group and a kit for detecting avian pathogenic escherichia coli based on an LAMP (Loop-Mediated Isothermal Amplification) method, and belongs to the technical field of biological detection. The primer group comprises an upstream outer primer F3, a downstream outer primer B3, an upstream inner primer FIP and a downstream inner primer BIP, the kit comprises the primer group: an upstream outer primer F3, a downstream outer primer B3, an upstream inner primer FIP and a downstream inner primer BIP. The novel avian pathogenic Escherichia coli LAMP primer group is autonomously designed, is used for detecting avian pathogenic Escherichia coli based on a loop-mediated isothermal amplification method and can be applied to kit detection, a result can be obtained by observing color change, and a large amount of time is saved; meanwhile, the lowest detection limit of target strains reaches 3.6 * 100 CFU/mL, and the detection limit is remarkably improved compared with that of a traditional PCR method.

Primer and probe set for detecting sheep abortion-causing bacteria and application of primer and probe set

Resumen de: CN121826192A

The invention provides a primer and a probe group for detecting sheep abortion-causing bacteria and application of the primer and the probe group, and belongs to the technical field of biological detection. The primer and probe group is characterized in that the sequence of the primer and probe group for detecting the listeria is shown as SEQ ID NO.1-3, and the sequence of the probe group for detecting the listeria is shown as SEQ ID NO.2-3; according to the primer for detecting the salmonella abortus, the sequence of the probe group is as shown in SEQ ID NO.4-6; the sequences of the primer and the probe group aiming at Brucella detection are shown as SEQ ID NO.7-9; the sequence of a primer and a probe group for detecting chlamydia psittaci is as shown in SEQ ID NO.10-12. The invention further discloses a kit for detecting chlamydia psittaci. The method has the characteristics of visualization, high sensitivity, strong specificity, simplicity and convenience in operation, short time consumption and the like, realizes rapid differential diagnosis of four sheep bacterial abortion diseases through one-time PCR amplification, and has important advantages in the field of sheep disease diagnosis and identification.

Strain identification and drug sensitivity detection kit as well as preparation method and application thereof

Resumen de: CN121801685A

The invention belongs to the technical field of strain identification and drug sensitivity detection, and discloses a strain identification and drug sensitivity detection kit and a preparation method and application thereof, the kit comprises a drug sensitivity disc, a pre-enrichment chromogenic culture medium, a chromogenic culture medium, sterile water and a diluent; the drug sensitive disc body comprises an enterobacteriaceae area, an enterococcus area, a staphylococcus area, a pseudomonas aeruginosa area and an acinetobacter baumannii area; the enterobacteriaceae area is provided with enterobacteriaceae identification culture medium micropores and drug micropores, the enterococcus area is provided with enterococcus identification culture medium micropores and drug micropores, the staphylococcus area is provided with staphylococcus identification culture medium micropores and drug micropores, and the pseudomonas aeruginosa area is provided with pseudomonas aeruginosa identification culture medium micropores and drug micropores. An acinetobacter baumannii identification culture medium micropore and a medicine micropore are matched in the acinetobacter baumannii area; the problems that an existing drug sensitivity test mode is complex in operation, high in cost and low in accuracy of identification and detection results are solved.

Composite material GZHMU-2 (at) Ag and application of composite material GZHMU-2 (at) Ag in preparation of self-cleaning biosensor

Resumen de: CN121797397A

The invention belongs to the technical field of organic materials, and particularly relates to a composite material GZHMU-2 (at) Ag and application of the composite material GZHMU-2 (at) Ag in preparation of a self-cleaning biosensor. The composite material GZHMU-2 (at) Ag is synthesized by loading AgNPs on GZHMU-2 through a method of reducing silver nitrate through ultraviolet irradiation, and the GZHMU-2 (at) Ag can generate active oxygen through photocatalysis, so that an antibacterial effect is achieved, and the composite material has a killing effect on escherichia coli and staphylococcus aureus. Based on a metal covalent organic framework modified by silver nanoparticles, a carboxyl functionalized SA31 aptamer is fixed on GZHMU-2 (at) Ag through an amidation reaction, a self-cleaning electrochemical biosensor for sensitive detection and real-time inactivation of bacteria is developed, the photocatalytic and electrochemical properties of the sensor are remarkably enhanced, and the sensor has a wide application prospect. The method has important potential for field monitoring and photocatalytic disinfection of pathogens in practical application.

Reusable dual-mode aptamer biosensor for detecting clostridium perfringens

Nº publicación: CN121805581A 07/04/2026

Solicitante:

JIANGSU ACAD OF AGRICULTURAL SCIENCES
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Resumen de: CN121805581A

The invention discloses a reusable dual-mode aptamer biosensor for detecting clostridium perfringens, which is characterized by comprising a competitive chain CP-C, a CeO2 (at) PANI nano composite material, a closed bipolar electrode system and an aptamer functionalized magnetic bead probe. According to the invention, the high-affinity nucleic acid aptamer is combined with the specific extracellular electron transfer mechanism of the clostridium perfringens viable bacteria for the first time, a new viable bacteria detection sensing strategy free of nucleic acid extraction and target gene amplification is constructed, and the biosensor can specifically recognize the viable clostridium perfringens, and can be used for detecting the viable bacteria of the clostridium perfringens. According to the present invention, with the detection method, the false positive result caused by the existence of dead bacteria is avoided, the detection can be rapidly completed within 3 min, the voltage signal and the G value signal are mutually proved, the false positive or false negative of the single signal detection is effectively avoided, the detection limit is as low as 2.50 CFU/mL, and the excellent anti-interference ability in the complex food matrix such as chicken and milk is provided; the core element ITO glass electrode can be continuously and repeatedly used for 12 times, and the performance is not obviously attenuated.