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57
resultados
Última actualización
16/10/2026 [07:49:00]
Resultados 25 a 50 de 57
Resumen de: CN122445823A
The invention relates to the technical field of microbiological detection, in particular to a primer probe composition and a kit for simultaneously detecting salmonella and acinetobacter baumannii and application of the primer probe composition and the kit, the primer probe composition comprises a primer pair and a probe for detecting salmonella and a primer pair and a probe for detecting acinetobacter baumannii; the nucleotide sequence of the primer pair for detecting the salmonella comprises a sequence as shown in SEQ ID NO.1-2, and the nucleotide sequence of the probe for detecting the salmonella comprises a sequence as shown in SEQ ID NO.3; the nucleotide sequence of the primer pair for detecting the acinetobacter baumannii comprises a sequence as shown in SEQ ID NO.4-5, and the nucleotide sequence of the probe for detecting the acinetobacter baumannii comprises a sequence as shown in SEQ ID NO.6. The invention further discloses a kit for detecting the acinetobacter baumannii. According to the invention, salmonella and acinetobacter baumannii can be quantitatively detected rapidly, sensitively and simultaneously in a single reaction system in a non-interfering manner, and the kit has important application value for clinical rapid screening, hospital infection control and food safety risk monitoring.
Resumen de: CN122445651A
The invention discloses a nucleic acid aptamer combination for detecting escherichia coli O157 and a biosensor of the nucleic acid aptamer combination. The nucleic acid aptamer combination for detecting the escherichia coli O157 is composed of nucleic acid aptamers shown in SEQ ID NO: 1-3, the nucleic acid aptamer combination has multiple recognition sites and can perform multi-molecule joint detection on bacterial cells to be detected through the synergistic effect, and the affinity and specificity of the aptamers to target substances are improved; when being applied to a biosensor for detection and used as an important recognition element, the Escherichia coli O157 sensor realizes high-sensitivity detection on Escherichia coli O157 in a biosensing platform, the accuracy reaches 100%, the capturing efficiency on viable bacteria cells is effectively improved, and the sensitivity of the sensor and the detection accuracy are further improved. Meanwhile, the invention provides a method for specifically detecting Escherichia coli O157 by the nucleic acid aptamer combined functionalized biosensor, and more methods and means are provided for detection of food-borne bacteria.
Resumen de: CN122428049A
The invention relates to the technical field of microbial molecular diagnosis, in particular to a detection platform capable of activating Cas12a trans-cleavage activity without a PAM sequence and application of the detection platform in rapid detection of aquatic pathogenic bacteria vibrio harveyi. According to the invention, the PAM dependence limitation is broken through, the efficient activation of Cas12a under the condition of no PAM sequence is realized by optimizing dsDNA target positioning and crRNA spacer region composition, the dsDNA does not need to be converted into ssDNA, and the target selection range of a CRISPR-Cas12a detection system is expanded. According to the method, the detection process is simplified, pretreatment steps such as thermal denaturation and enzymolysis are omitted, reagent types and operation links are reduced, the sample pollution risk is reduced, meanwhile, the total detection time consumption is shortened, and the method is more suitable for on-site rapid detection. The method is high in clinical application value, in clinical sample detection of trachinotus ovatus infection, the detection result of a PFCD platform is completely consistent with that of qPCR (existing molecular diagnosis'gold standard '), infected individuals can be accurately recognized, and reliable technical support is provided for early diagnosis, prevention and control of aquaculture diseases.
Resumen de: CN122405852A
The invention relates to a specific primer combination for detecting glacier melt water transmission related pathogenic microorganisms. The specific primer combination comprises a vibrio cholerae primer Seq1 with a target gene of ompW, a bacillus cereus primer Seq4 with a target gene of gyrB, a clostridium botulinum primer Seq7 with a target gene of bond/A, and a legionella pneumophila primer Seq10 with a target gene of mip. The method is high in sensitivity and good in environmental applicability, not only can realize joint detection of spreading related pathogenic microorganisms in the glacier environment, but also can be used for analyzing migration and diffusion paths in different environmental media, and provides technical support for glacier melt water spreading process and downstream biological safety risk assessment.
Resumen de: CN122409630A
The invention discloses a salmonella dual-mode detection method based on medium-temperature Argonaute driving, and belongs to the technical field of food safety detection. According to the method, the functionalized DNA hydrogel internally packaged with an asymmetric RPA system, a CbAgo system and nano-enzyme is constructed, cascade signal amplification, DNA hydrogel phase change and nano-enzyme signal output links are integrated, and rapid detection of salmonella is realized under the assistance of gravity. The invention also independently develops portable detection equipment and an intelligent application program, realizes automatic image acquisition and analysis, has the advantages of high sensitivity, strong specificity and simplicity and convenience in operation, and is suitable for on-site rapid detection of food safety.
Resumen de: CN122405858A
The invention relates to an oligonucleotide primer probe composition for detecting food-borne pathogenic pseudomonas bacteria (pseudomonas alcaligenes and pseudomonas buchneri) based on an ERA fluorescence method or an ERA test strip method, and a detection method using the composition. The pseudomonas alcaligenes or pseudomonas buchneri in the sample can be rapidly, specifically and sensitively detected, and the method is easy and convenient to operate, short in reaction time and free of complex temperature control equipment, and can be widely applied to rapid screening, pollution monitoring and risk early warning of food-borne pathogenic pseudomonas in various food samples such as milk and dairy products.
Resumen de: CN122405862A
The invention provides a primer for detecting Pseudomonas tolaasi, the primer comprises a forward primer and a reverse primer, the nucleotide sequence of the forward primer is as shown in SEQ ID No: 1, and the nucleotide sequence of the reverse primer is as shown in SEQ ID No: 2. The method for detecting the Pseudomonas tolaasi comprises the following steps: extracting genome DNA (Deoxyribonucleic Acid) of a sample to be detected, taking the DNA as a template, carrying out PCR (Polymerase Chain Reaction) amplification by utilizing the forward primer and the reverse primer, carrying out agarose gel electrophoresis detection on a PCR amplification product, and judging whether the sample to be detected contains the Pseudomonas tolaasi or not. The invention also provides an application of the primer for detecting the Pseudomonas tolaasi, and the primer is used for preparing a kit for detecting the Pseudomonas tolaasi, and the application of the primer for detecting the Pseudomonas tolaasi is used for detecting the Pseudomonas tolaasi. The primer for detecting Pseudomonas tolaasi, which is designed by the invention, has the advantages of strong specificity, no need of isolated culture and biochemical identification, short detection period, high efficiency, good universality and wide application prospect in allusion to a specific gene of Pseudomonas tolaasi.
Resumen de: CN122404548A
The invention discloses a monoclonal antibody for detecting surface protein SapA of campylobacter fetus and application of the monoclonal antibody. The monoclonal antibodies are named as 4F6 and 7B3 respectively, and amino acid sequences of variable regions of heavy chains and light chains of the two antibodies are disclosed. The monoclonal antibody prepared by the invention can specifically recognize the surface protein SapA of the campylobacter fetus, has better specificity, and does not have cross reaction with non-campylobacter such as standard escherichia coli, salmonella, listeria, klebsiella pneumoniae, staphylococcus aureus, streptococcus and the like; no cross reaction with campylobacter jejuni, campylobacter colon and other campylobacter is generated; the test strip prepared by combining the monoclonal antibody and colloidal gold can realize on-site rapid detection of the campylobacter fetus, the detection limit is low, the sensitivity is high, and an immunological method capable of specifically detecting the campylobacter fetus is successfully developed.
Resumen de: CN122405789A
The invention belongs to the technical field of biological detection, and particularly relates to a detection system for food-borne pathogenic bacteria and application of the detection system. The detection system for the food-borne pathogenic bacteria comprises a composite response material, hyaluronic acid hydrogel containing L-ascorbic acid-2-trisodium phosphate and bacteriophage, the composite response material is prepared from ZIF-90 nano particles, hyaluronidase and alkaline phosphatase, wherein the hyaluronidase and the alkaline phosphatase are encapsulated in the ZIF-90 nano particles. According to the invention, a dual-mode paper-based visual detection system based on combination of phage specific recognition and a ZIF-90 response release mechanism is constructed, and high-specificity detection of target pathogenic bacteria is realized. Compared with a nucleic acid amplification or immunological method which has the defect that live bacteria and dead bacteria are difficult to distinguish, the method provided by the invention only generates signal response to the live bacteria which can be infected by the bacteriophage, so that the authenticity and the reliability of a detection result are remarkably improved.
Resumen de: CN122405853A
The invention belongs to the technical field of biological detection, and discloses a quadruple PCR (Polymerase Chain Reaction) detection kit and method for detecting four bovine-derived respiratory tract pathogenic bacteria. The kit and the method comprise a primer pair for detecting pasteurella multocida with nucleotide sequences shown as SEQ ID NO.1 and SEQ ID NO.2, a primer pair for detecting mannheimia haemolytica with nucleotide sequences shown as SEQ ID NO.3 and SEQ ID NO.4, and a primer pair for detecting mannheimia haemolytica with nucleotide sequences shown as SEQ ID NO.3 and SEQ ID NO.4, the nucleotide sequences of the primer pair are as shown in SEQ ID NO.5 and SEQ ID NO.6, and the primer pair is used for detecting klebsiella pneumoniae; the nucleotide sequences of the primer pair are as shown in SEQ ID NO.7 and SEQ ID NO.8, and the primer pair is used for detecting mycoplasma bovis. The quadruple PCR detection kit and the quadruple PCR detection method provided by the invention can amplify DNAs of four pathogens including Pm, Mh, Kp and MB in the same reaction system, and have the characteristics of high detection efficiency, high sensitivity and strong specificity.
Resumen de: CN122382225A
The invention discloses a super-multiplex PCR (polymerase chain reaction) primer combination capable of simultaneously detecting 35 pathogenic bacteria and fecal indicator bacteria and application of the super-multiplex PCR primer combination. The super-multiplex PCR primer combination comprises 35 pairs of target specific primers, and nucleotide sequences of the 35 pairs of target specific primers are shown as SEQ ID NO.1-70. The method for detecting 35 pathogenic bacteria and fecal indicator bacteria comprises the following steps: extracting DNA of a sample to be detected to obtain a DNA mixture of a target sample; carrying out ultra-multiplex PCR amplification on the target sample DNA mixture to obtain a target amplification product, and carrying out high-throughput sequencing to obtain a target amplification product sequencing result; and detecting and judging the to-be-detected sample. According to the method, generation of dimers among the primers and nonspecific amplification are effectively reduced, homogenized amplification of 35 targets in a single reaction system is realized, and the method can be applied to the fields of food-borne pathogenic bacterium detection, public health emergency detection and the like.
Resumen de: CN122385882A
The invention provides a composite probe and a test strip for detecting food-borne pathogenic bacteria and application of the composite probe and the test strip, and belongs to the technical field of food-borne pathogenic bacteria detection. The composite probe comprises a bacteriophage functional capture probe and a ZMPAP signal composite probe; the bacteriophage functional capture probe contains a specific inactivated bacteriophage aiming at the food-borne pathogenic bacteria; the ZMPAP signal composite probe is prepared from a ZIF-67 metal organic framework material, methylene blue, polydopamine, gold nanoparticles and porcine immune globulin G. Compared with a single signal detection technology, the method disclosed by the invention has visual qualitative and accurate quantitative capabilities, is strong in specificity and good in repeatability, and provides a core technical support for on-site rapid prevention and control of the food-borne pathogenic bacteria.
Resumen de: CN122385571A
The invention discloses a single-tube multiple Raman/visual detection method and kit for pathogenic bacteria based on Cas12a and an in-situ crRNA synthesis strategy, and belongs to the technical field of food safety detection. By designing single-molecule allosteric hairpin DNA containing a target recognition region, a T7 promoter and a crRNA template, 16S rRNA of specific response target bacteria, hairpin structure recombination and crRNA in-situ synthesis are started, and cis-and trans-cleavage of Cas12a is guided, so that rapid visual detection of the total bacteria amount and SERS identification and quantitative detection of different pathogenic bacteria targets are realized. The method solves the problem of insufficient qualitative accuracy and quantitative sensitivity caused by the interference of a biological matrix in the existing rapid detection method of the pathogenic bacteria, overcomes the dependence of a CRISPR/Cas12a method on a target PAM site, and greatly widens the detection range of the nucleic acid target of the food-borne pathogenic bacteria. The Raman/visual dual-mode detection system adapts to a field rapid detection scene, the detection limit is as low as the single cell level, the specificity and reproducibility are excellent, and the Raman/visual dual-mode detection system can be used for scenes such as food safety control and live pathogenic bacterium diagnosis.
Resumen de: CN122382221A
The invention relates to the technical field of nucleic acid detection, in particular to a food-borne pathogenic bacterium rapid detection method based on CRISPR-Cas12a. According to the method, after genome DNA is extracted from a food sample, bridging primers of an upstream primer combination identification gene segment and a downstream primer combination virulence gene segment are provided, the two segments are located on the same continuous DNA molecule at the interval of 50-1500 bp, and the two segments are located on the same continuous DNA molecule; amplifying for 20-40 minutes at the constant temperature of 37 DEG C, and amplifying to obtain a chimeric amplicon only when the two segments are linked to the same molecule; and activating Cas12a trans-cleavage activity by combining crRNA of a guide sequence spanning a junction site with a chimeric amplicon, cleaving a single-stranded DNA reporter probe and detecting a signal. In the single-tube constant-temperature reaction, ligase is not needed, the linkage state of the gene and the virulence gene is directly confirmed and identified, the detection limit is 10-100 CFU/mL, and the time consumed in the whole process is less than 60 minutes.
Resumen de: CN122382226A
The invention discloses a salmonella detection system based on RPA-CRISPRCas12a and application of the salmonella detection system. The detection system comprises an RPA (recombinase polymerase amplification) primer pair, a probe, crRNA (ribonucleic acid) and LbCas12a protein, the sequences of the RPA amplification primer pair are as shown in SEQ ID NO. 1 and SEQ ID NO. 2; the sequence of the probe is as shown in SEQ ID NO.7; and the sequence of the crRNA is as shown in SEQ ID NO. 8. The RPA-CRISPR/Cas12a visual detection system has the advantages of high sensitivity, strong specificity, simplicity and convenience in operation, rapidness in detection and the like, the lowest detection limit of salmonella reaches 1 fg/mu L, and stable and repeatable fluorescence signals and visual results can still be generated under the concentration. The system effectively overcomes the defects of a traditional detection method, is suitable for on-site rapid screening and risk early warning of salmonella in a food chain, provides technical support for food safety supervision, and also provides a reference technical scheme for rapid detection of other food-borne pathogenic bacteria.
Resumen de: CN122382222A
The invention discloses a method for rapidly detecting streptococcus suis or/and salmonella based on an RAA-EXO technology and application of the method. According to the method, specific RAA primers and EXO probes are designed and screened aiming at streptococcus suis recN genes and salmonella invA genes, the sequences of the specific RAA primers and the EXO probes are shown as SEQ ID NO.3, 5 and 10 and SEQ ID NO.14, 15 and 20 respectively, a single or double RAA-EXO detection system for the streptococcus suis and the salmonella is established, detection can be completed within 20 minutes under the condition of the constant temperature of 39-41 DEG C, and the detection sensitivity is high. And no cross reaction with common bacteria such as escherichia coli, staphylococcus aureus and the like exists. Results can be visually judged through a fluorescence curve or a blue light instrument, an effective tool is provided for rapid, sensitive and on-site detection of streptococcus suis and salmonella, and the kit has important application value in the fields of food safety and epidemic disease prevention and control.
Resumen de: CN122356274A
The invention relates to the field of biological medicine, in particular to an O45 Escherichia coli detection antibody and an application method thereof. The antibody is a monoclonal antibody or an antigen binding fragment thereof, and comprises a heavy chain CDR sequence as shown in SEQ ID NO: 1-3 and a light chain CDR sequence as shown in SEQ ID NO: 4-6. The invention also provides a nucleic acid molecule for coding the antibody, an expression vector, a host cell and a preparation method thereof. Specific identification results show that the antibody is specifically combined with O45 Escherichia coli, has no cross reaction with O157, O26, O103, O111, O121, O145 and other Escherichia coli serotypes and O55 related serotypes, and also has no cross reaction with common food-borne pathogenic bacteria. The detection limit of an indirect ELISA detection method established based on the antibody to O45 Escherichia coli can reach 1 * 10 < 2 > CFU/mL, and the whole detection process can be completed within 24 hours. The monoclonal antibody provided by the invention can be applied to preparation of detection kits, detection test paper and other products, and is suitable for rapid screening of O45 Escherichia coli in import and export food.
Resumen de: EP4772522A2
Disclosed herein are methods and compositions for detecting one or more pathogens that cause atypical pneumonia. Detectable pathogens include Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophila.
Resumen de: CN122344612A
The invention provides a fluorescent rapid detection reagent and application thereof, and belongs to the technical field of bacterial detection. The rapid fluorescent detection reagent provided by the invention comprises a bacterium specific aptamer, an initiation chain and hairpin structures H1, H2 and H3. By establishing a fluorescent specific amplification detection system with strong specificity and high sensitivity, a fluorescent signal amplification marker aiming at staphylococcus aureus and pseudomonas aeruginosa susceptible to rodents and a handheld visual detection technology of the fluorescent signal amplification marker are developed, and the fluorescent signal amplification marker and the handheld visual detection technology are prepared into a detection reagent, so that rapid and sensitive detection of target bacteria is realized, and the detection efficiency is improved. The method has important significance on screening detection and quality control of pathogenic microorganisms of experimental animals.
Resumen de: CN122326781A
The invention relates to the field of gene detection, in particular to a food-borne pathogenic bacterium single-tube multiple detection sequence combination based on an asymmetric ratio and melting curve, a kit, a detection method and application. The invention provides a sequence combination for detecting food-borne pathogenic bacteria. The sequence combination comprises a forward primer, a reverse primer, a specific probe and a reverse partial complementary melting curve probe. The invention further provides a kit for detecting the food-borne pathogenic bacteria, the problems of system competitive inhibition and cross mismatch under a complex genetic background are solved by adjusting an asymmetric ratio, and a detection method for detecting type 5 diarrheagenic Escherichia coli, salmonella, listeria monocytogenes and staphylococcus aureus through a single tube is constructed. The method does not need to add a complex hardware channel or expensive detection equipment, has the advantages of high detection flux, strong specificity and low instrument cost, and is suitable for application and popularization of low-resource grass-roots institutions, customs and first-line food safety supervision.
Resumen de: CN122303462A
The invention provides a primer/probe composition for simultaneously detecting shigella and salmonella, a kit and application, and belongs to the technical field of gene detection. The invention comprises a primer/probe set for detecting shigella and a primer/probe set for detecting salmonella. The invention has the advantages of high detection sensitivity, strong specificity, high dual synchronous detection efficiency, good repeatability, good stability and low pollution risk, and is especially suitable for low-load sample detection and rapid screening of monkey group samples.
Resumen de: CN122303457A
The invention discloses a double-channel quadruple ddPCR detection method and kit for simultaneously detecting diarrhea bacteria in sewage based on a proportional fluorescent probe strategy. Compared with the traditional quadruple detection method with four different fluorescence channels, the quadruple ddPCR detection method disclosed by the invention realizes quadruple detection only through the concentration difference of the probes in the two fluorescence channels. Sewage is used as a key interface between excrement and the environment, is an important place for pathogen storage and transmission, and is also a sentry for early warning. According to the double-channel quadruple ddPCR method established by the invention, salmonella spp. SM, shigella spp. SH, vibrio parahaemolyticus VP and vibrio cholerae VC in the sewage can be simultaneously detected, a sewage monitoring technical method is perfected, potential epidemic situation risks can be captured before disease outbreak, and public health prevention and control measures can be taken in time.
Resumen de: CN122303454A
The invention discloses a detection method and application of F17 escherichia coli, fimbriae virulence gene ctg04543 or ctg04549 of the F17 escherichia coli is taken as a target, a fluorescent quantitative probe is designed according to a nucleotide sequence, genome DNA of the F17 escherichia coli is taken as a template, the fimbriae virulence gene ctg04543 or ctg04549 of the F17 escherichia coli is detected through fluorescent quantitative PCR, and the F17 escherichia coli in a sample can be quantitatively detected according to a fitted standard curve. The F17 Escherichia coli detection method provided by the invention has the characteristics of simplicity, rapidness, quantitation, high sensitivity and high specificity, can realize quantitative detection of F17 Escherichia coli within 2-3 hours, can meet the detection requirements under various conditions of laboratory research, animal pathogen diagnosis and the like, and has wide application prospects and extremely high market implementation possibility.
Resumen de: CN122282714A
The invention discloses an embedded whole-cell biosensor, a preparation method of the embedded whole-cell biosensor and application of the embedded whole-cell biosensor in machine learning assisted mercury ion detection, and belongs to the technical field of sensor materials and detection. According to the sensor, escherichia coli (E. coli) for expressing green fluorescent protein (GFP) is encapsulated in a polyacrylamide/hyaluronic acid (PAAM/HA) hydrogel matrix, so that the sensor can realize high-selectivity and high-sensitivity detection on mercury ions, can effectively eliminate interference of competitive metal ions, can realize complete regeneration through EDTA-2Na treatment, and can be used for detecting mercury ions in a high-sensitivity manner. The device can be repeatedly used under the condition that the performance is not lost; stable fluorescence response as long as three weeks can be maintained; excellent biocompatibility and biodegradability are achieved, and the risk of secondary pollution is reduced to the maximum extent; and rapid on-site analysis is facilitated.
Nº publicación: CN122278855A 26/06/2026
Solicitante:
UNIV YANGZHOU
\u626C\u5DDE\u5927\u5B66
Resumen de: CN122278855A
The invention discloses sgRNA (small guide RNA), an RPA (recombinase polymerase amplification) primer pair and application of the sgRNA and the RPA primer pair in campylobacter coli detection. The sgRNA contains a target sequence of which the nucleotide sequence is as shown in SEQ ID NO.1 or SEQ ID NO.2. The lowest detection limit of the detection method is 100 CFU/mL, the specificity is high, the detection operation is simple, the detection method is not limited by site equipment and professional ability of operators, the application range is wide, the detection method can be used for rapidly detecting campylobacter colons in actual excrement samples, and the detection result is accurate.
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