Alerta

Compositions and methods for detecting gastrointestinal pathogens

Resumen de: AU2026208127A1

Compositions, methods, kits, and uses are provided for detecting the presence of Salmonella, Shigella, Campylobacter, and Shigatoxigenic Escherichia coli (STEC) in samples. In some embodiments, the compositions, methods, kits, and uses comprise one or more oligonucleotides or the use of one or more oligonucleotides. ul u l

Methods and Compositions for Detecting Virulent and Avirulent Escherichia coli Strains

Resumen de: US20260242877A1

Disclosed herein are methods for detecting virulent Shiga toxin-producing E. coli (STEC) strains O26, O103, O121, and O111 in a biological sample comprising the steps of: (i) enriching the bacterial concentration of the biological sample to result in an enriched sample; (ii) isolating DNA from said enriched biological sample; and (iii) detecting virulent strain in said isolated DNA sample via real-time PCR and a melt curve assay. Also disclosed are primers for said assay, as well as kits comprising said primers.

Double-probe system, test strip and detection method for simultaneously detecting listeria monocytogenes and escherichia coli O157: H7

Resumen de: CN122525123A

The invention discloses a double-probe system, a test strip and a detection method for simultaneously detecting Listeria monocytogenes and Escherichia coli O157: H7, the constructed double-probe system comprises an AIENPs-FAM fluorescent nanoprobe and an AIENPs-digoxin fluorescent nanoprobe, the invention further discloses an immunochromatographic test strip corresponding to the double-probe system, and the invention further discloses a constructed RPA-CRISPR-LFCS method based on AIENPs, which can be used for simultaneously detecting Listeria monocytogenes and Escherichia coli O157: H7. High-sensitivity screening of the listeria monocytogenes LM and the escherichia coli O157: H7 can be achieved at the same time, the detection limit of the LM is 29 fg/mu L (223 CFU/mL), and the detection limit of the escherichia coli O157: H7 is 25.1 fg/mu L (195 CFU/mL).

Polypeptide antigen based on poultry clostridium perfringens and indirect ELISA antibody detection reagent

Resumen de: CN122520812A

The invention belongs to the technical field of polypeptides, and particularly relates to a polypeptide antigen based on avian clostridium perfringens and an indirect ELISA antibody detection reagent, and the amino acid sequence of fusion protein polypeptide serving as the antigen is SEQ ID NO: 1. The fusion protein polypeptide has good immunoreactogenicity, and the prepared indirect ELISA detection reagent can effectively detect the dynamic change trend of the antibody level within 24 weeks after subunit vaccine immunization, so that a reliable technical means is provided for detection and monitoring of the antibody titer in the vaccine clinical application process; the important technical support is provided for the prevention and control of the clostridium perfringens disease of the poultry.

Salmonella specific antigen fragment, gene, kit and application thereof in detection of salmonella antibody in chicken serum

Resumen de: CN122520725A

The invention discloses a salmonella specific antigen fragment, a gene, a kit and application of the salmonella specific antigen fragment in detection of a salmonella antibody in chicken serum. The amino acid sequence of the salmonella specific antigen PagN fragment is as shown in SEQ ID NO. 1. The serological diagnostic value of the salmonella specific antigen PagN fragment in salmonella chicken flocks infection is disclosed for the first time, the salmonella specific antigen PagN fragment is used as a coating antigen of indirect ELISA, and a salmonella antibody indirect ELISA detection method is established. The PagN fragment of the outer membrane protein of the salmonella can specifically recognize a salmonella antibody, can avoid detection obstacles caused by serotype difference, can rapidly lock salmonella infection, and is suitable for clinical scenes needing rapid preliminary screening. The method can be used for detecting salmonella antibodies in chicken flocks, and provides powerful technical support for epidemiological investigation of salmonella and precise prevention and control of salmonella infection.

Primer probe combination for analyzing and detecting clostridium perfringens by using asymmetric PCR (Polymerase Chain Reaction) in combination with melting curve and detection method of clostridium perfringens

Resumen de: CN122521876A

The invention discloses a primer probe combination for analyzing and detecting clostridium perfringens by using asymmetric PCR (Polymerase Chain Reaction) in combination with a melting curve and a detection method of the clostridium perfringens, and belongs to the technical field of pathogenic microorganism detection. The invention discloses a primer probe combination for analyzing and detecting clostridium perfringens by using asymmetric PCR (Polymerase Chain Reaction) in combination with a melting curve. The sequence of the primer probe combination is as shown in SEQ ID NO.1-3. The asymmetric PCR and the probe melting curve are combined to generate a synergistic effect, the asymmetric PCR provides a high-quality single-chain template for analysis of the probe melting curve, and the problem of low hybridization efficiency caused by rapid double-chain renaturation in the symmetric PCR is solved; a single-chain product generated by asymmetric PCR is fully utilized in probe melting curve analysis, and high-specificity and high-sensitivity detection is realized.

Liquid chip nucleic acid detection kit and detection method for simultaneously detecting 10 experimental animal intestinal pathogens

Resumen de: CN122521874A

The invention discloses a liquid chip nucleic acid detection kit and a detection method for simultaneously detecting 10 experimental animal intestinal pathogens. The 10 pathogenic bacteria comprise salmonella typhimurium, Tyzz pathogen, klebsiella pneumoniae, pseudomonas aeruginosa, citrobacter rodent, helicobacter hepatae, klebsiella oxytoca, staphylococcus aureus, corynebacterium bovis and yersinia pseudotuberculosis. Primers are designed for specific target genes of various pathogenic bacteria, the 5'end of an upstream primer is connected with a TAG sequence, and the 5 'end of a downstream primer is labeled with biotin; after multiple PCR amplification, a product is hybridized with MagPlex-TAG microspheres with corresponding numbers, and a liquid phase chip system is used for detecting fluorescence signals. The method disclosed by the invention can be used for simultaneously detecting the 10 pathogenic bacteria in the same reaction system, has the advantages of high throughput, high sensitivity, high specificity, good repeatability and the like, and provides an efficient monitoring tool for quality control of experimental animal microorganisms.

Fluorescence immunochromatography rapid detection system for multiple pathogenic bacteria of agricultural products

Resumen de: CN122524763A

The invention relates to the technical field of fluorescence immunochromatography, and discloses an agricultural product multiple pathogenic bacterium fluorescence immunochromatography rapid detection system, which comprises a sample pretreatment module, a fluorescent microsphere labeling module, a multi-channel chromatography module, an optical detection module, a data processing analysis module and a result output and early warning module, dark current correction and gain adjustment are performed on acquired signals through a signal amplification unit in the optical detection module, and the chromatographic film is monitored by using the multispectral imaging unit, so that whether excitation light intensity attenuation and wave band drifting occur at a fluorescence signal acquisition position of the chromatographic film or not can be detected in real time; the accuracy of fluorescence immunochromatography rapid detection of multiple pathogenic bacteria of agricultural products is ensured, and the reading error of fluorescence signals is reduced.

Salmonella detection method based on RPA-HRCA cascade amplification platform and application

Resumen de: CN122521878A

The invention discloses a salmonella detection method based on an RPA-HRCA cascade amplification platform and application of the salmonella detection method. The method comprises the following steps: performing recombinase polymerase amplification (RPA) on target DNA (Deoxyribonucleic Acid) from salmonella, wherein the 5'end of one primer is modified with a phosphate group, then lambda excision enzyme is used for selectively degrading ssDNA with the 5 'end modified with the phosphate group in an amplification product, complementary ssDNA which is not phosphorylated is reserved, then the reserved ssDNA is hybridized with a padlock probe, the padlock probe is cyclized, then the cyclized padlock probe is used as a template, and the fluorescence intensity of the padlock probe is enhanced. A molecular beacon with a polythymine tail is used for starting hyperbranched rolling circle amplification HRCA, and then a fluorescence signal of an amplification product is detected. According to the invention, RPA and HRCA are combined for cascade amplification of detection signals, and high-sensitivity detection of sensitive and specific pathogens can be realized under mild conditions.

In situ CHROMATIC BIOSENSOR

Resumen de: US20260224161A1

0000 A biosensor for real-time monitoring of a wound can provide valuable data regarding the presence of bacteria. A nanofibrous biosensor is provided for monitoring wound status at the point of care. The colorimetric biosensor changes color in response to low levels of bacteria and fungi, making it visible to unaided and untrained eyes. A colorimetric probe is a hemicyanine dye that changes color from yellow to green in the presence of lipase. Dye is incorporated into a shell composition of core-shell nanofibers made of polyurethane and polyvinylpyrrolidone. As a means of increasing the biosensor's sensitivity, the alignment of nanofibers is controlled and a surfactant added to the shell (e.g., Tween 80). Alignment of nanofiber enables better localization, and Tween 80 increases lipase activity, which facilitates near immediate color changes above critical levels of Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and Candida aureus. All ESKAPEE bacteria are detected within 2 hours.

Method for detecting sterilization rate of listeria monocytogenes after atmospheric plasma treatment

Resumen de: CN122503475A

The invention discloses a method for detecting the sterilization rate of Listeria monocytogenes after atmosphere plasma treatment, and belongs to the field of microbiological detection, and the method specifically comprises the following steps: S1, dividing samples after cold atmosphere plasma treatment into an activity test group and a stress test group; s2, performing PMA treatment on the live test group, and then measuring qPCR (quantitative polymerase chain reaction); if the detection result is positive, the determination in the step S3 is continued; and S3, detecting the expression quantity of the target gene of the stress test group, setting a control group, and calculating the target gene expression quantity ratio of the stress test group to the control group. According to the detection method provided by the invention, live test and marker detection are combined, and a dual evaluation system is established. The problem that in the prior art, due to the fact that cold atmosphere plasma treatment lacks evaluation on the resuscitation capacity of the listeria monocytogenes in the cold chain environment, potential food safety hazards are caused is solved. The method has the potential of being applied to food safety detection after CAP treatment.

Nucleic acid aptamer A.h-1. TR1 with high affinity to aeromonas hydrophila and application of nucleic acid aptamer A.h-1. TR1

Resumen de: CN122503392A

The invention discloses a nucleic acid aptamer A.h-1. TR1 with high affinity to aeromonas hydrophila and application of the nucleic acid aptamer A.h-1. TR1, and belongs to the technical field of nucleic acid aptamers. The nucleotide sequence of the aptamer is as shown in SEQ ID NO. 2. The stem-loop structure with a more stable structure is obtained by carrying out truncation-repeated transformation on an original aptamer A.h-1, deleting redundant sequences and carrying out in-situ copying on key base fragments. The affinity and dissociation constant of the aptamer and aeromonas hydrophila is 97.50 nM, the affinity is improved by 5.58 times compared with that of an original aptamer, and the aptamer has no obvious binding to common pathogenic bacteria such as escherichia coli, staphylococcus aureus and vibrio parahaemolyticus and is high in specificity. The aptamer disclosed by the invention can be used for constructing a biosensor and realizing rapid and sensitive detection of aeromonas hydrophila.

Pathogenic bacteria detection probe as well as preparation method and application thereof

Resumen de: CN122483780A

The invention discloses a pathogenic bacterium detection probe as well as a preparation method and application thereof. The pathogenic bacterium detection probe comprises a bacterial extracellular vesicle and a fluorescent nano-material coupled to the surface of the bacterial extracellular vesicle, the fluorescent nano material comprises fluorescent quantum dots. The probe depends on specific binding of host receptor related components reserved on the surface of extracellular vesicles and progeny bacteriophages to form a fluorescence labeling complex, rapid quantitative detection is achieved through nanoflow analysis, and the probe has the advantages of being high in detection speed and sensitivity, capable of distinguishing viable bacteria from dead bacteria and the like and has a good application prospect. The kit can be used for rapid screening and early diagnosis of clinical pathogenic bacteria.

Culture-free rapid detection method and device for pathogenic bacteria based on fluidic chip-SERS (Surface Enhanced Raman Scattering)

Resumen de: CN122483904A

The invention relates to the technical field of biological detection, in particular to a culture-free rapid detection method and device for pathogenic bacteria based on a fluidic chip-SERS (Surface Enhanced Raman Scattering), the device comprises a capture probe which is a magnetic bead-polypeptide probe formed by modifying a polypeptide probe on the surface of a maleimide magnetic bead through a sulfydryl-maleimide covalent bond; the centrifugal micro-fluidic chip is integrated with an enrichment module, an identification module and a drug sensitivity module which are connected through a micro-fluidic channel; and the detection unit is used for surface enhanced Raman spectrum detection. The method comprises the following steps: enriching pathogenic bacteria in the chip by using the capture probe; distributing the enriched bacteria to an identification module and a drug sensitivity module; the strains are rapidly identified through SERS; and a drug sensitive test is completed by detecting metabolic change through SERS. Specific capture, microfluidic automation and SERS rapid detection technology are deeply integrated, rapid enrichment and identification of pathogenic bacteria and integrated detection of parallel drug sensitivity are achieved, and the method has the advantages of being high in detection speed, high in sensitivity, full in process automation and the like.

Primer combination, kit and method for rapidly detecting main pathogens of tobacco

Resumen de: CN122484335A

The invention provides a primer combination, a kit and a method for rapidly detecting main pathogens of tobacco. The nucleotide sequences of the primers in the primer combination are as shown in SEQ ID NO. 1-8. The kit comprises the primer combination. According to the present invention, based on the multiple PCR-Cas12a detection principle, the trans-cleavage of the fluorophore-quenching group or the fluorophore-biotin probe is triggered, such that the simultaneous identification of the four main tobacco pathogens such as Pseudomonas sp. Pathogen, Phytophthora sp. Pathogen, Ralstania sp. Pathogen and Alternania sp. Pathogen causing tobacco bacterial wilt, black shank, brown spot, wildfire disease and other diseases can be achieved, and the multiple PCR-Cas12a detection method can be used for the simultaneous identification of the four main tobacco pathogens such as the Pseudomonas sp. Pathogen, the Phytophthora sp. Pathogen, the Ralstania sp. Pathogen and the Alternania sp. Pathogen. Therefore, the primer combination, the kit and the method for rapidly detecting the main pathogens of the tobacco, which are provided by the invention, have relatively strong matrix interference resistance, provide a sensitive and stable technical means for synchronous detection of the pathogens of the tobacco, and are applied to early diagnosis, rapid screening and effective prevention and control of tobacco diseases; powerful support can be provided for early diagnosis and accurate prevention a

Probe composition and application thereof in detection of pseudomonas aeruginosa

Resumen de: CN122487307A

The invention discloses a probe composition and application thereof in detection of pseudomonas aeruginosa, and belongs to the technical field of biological detection. The probe composition comprises a magnetic nanoprobe and a metal organic framework probe, and the magnetic nanoprobe is a covalent conjugate of magnetic beads and amino antibiotics; the preparation raw materials of the metal organic framework probe comprise a fluorescent hairpin probe and a metal organic framework material, and the metal organic framework material is a coordination polymer modified by an amino-containing polymer. The probe composition integrates enrichment and separation of nucleic acid of the pseudomonas aeruginosa, recognition and detection, can effectively overcome the defects of long time consumption, insufficient sensitivity, poor field applicability and the like of a traditional method, and realizes high-sensitivity, high-specificity and rapid detection of the pseudomonas aeruginosa.

COMPOSITIONS AND METHODS FOR DETECTING GASTROINTESTINAL PATHOGENS

Resumen de: US20260218268A1

Disclosed are nucleic acid oligomers, including amplification oligomers and detection probes, for detection of Yersinia enterocolitica, Vibrio spp., Shiga Toxin Expressing E. coli (STEC) O157, and Plesiomonas shigelloides target nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding formulations, reaction mixtures, and kits. Methods of synthesizing the nucleic acid oligomers are also disclosed.

Specific primer combination for detecting human exposure potential pathogenic bacteria in glacier environment

Resumen de: CN122466117A

The invention relates to a specific primer combination for detecting human exposed potential pathogenic bacteria in a glacier environment. The specific primer combination comprises a Klebsiella pneumoniae primer Seq1, a Pseudomonas aeruginosa primer Seq4, a Clostridium difficile primer Seq7 and a Legionella pneumophila primer Seq10, wherein the target gene of the Klebsiella pneumoniae primer Seq1 is wzc, the target gene of the Pseudomonas aeruginosa primer Seq4 is sigX, the target gene of the Clostridium difficile primer Seq7 is tcdB, and the target gene of the Legionella pneumophila primer Seq10 is mip. The method has the characteristics of high specificity and multiple targets, and can be effectively used for detection and risk identification of potential pathogenic bacteria in glacier environment samples.

Nucleic acid detection kit for on-site detection of multiple pathogens and application of nucleic acid detection kit

Resumen de: CN122466115A

The invention provides a nucleic acid detection kit for on-site detection of multiple pathogens and application, and relates to the technical field of molecular biology. The invention provides a nucleic acid detection kit for on-site detection of multiple pathogens, which comprises a primer probe for detecting staphylococcus aureus, a primer probe for detecting pseudomonas aeruginosa, a primer probe for detecting legionella pneumophila and a primer probe for detecting haemophilus influenzae, the detection kit provided by the invention can achieve relatively high detection sensitivity to four pathogenic bacteria, and the specificity and repeatability of the detection kit both meet the detection requirements. The kit disclosed by the invention has relatively high stability and can be stored for more than one year at normal temperature.

Application of SNP (Single Nucleotide Polymorphism) site in 3 'UTR (Untranslated Region) region of GRIA1 (Growth Ribonucleic Acid 1) gene in sheep anti-diarrhea molecular breeding and detection primer and method thereof

Resumen de: CN122466101A

The invention discloses application of an SNP (Single Nucleotide Polymorphism) site in a 3 'UTR (Untranslated Region) region of a GRIA1 gene in sheep anti-diarrhea molecular breeding as well as a detection primer and a detection method of the SNP site, belongs to the technical field of livestock molecular breeding, and discloses a single nucleotide polymorphism site NC019462.1: g.62655964Cgt located in the 3' UTR region of the GRIA1 gene of sheep for the first time; and G. The site can regulate and control the gene expression by influencing the binding capacity of oar-miR-30a-3p and the GRIA1 gene, so that the gene expression is associated with the F17 escherichia coli infection resistance of sheep. The invention also provides a specific primer pair for detecting the SNP site and a PCR (Polymerase Chain Reaction) detection method. By utilizing the invention, individuals with strong disease resistance can be screened by detecting the genotype of the site of the sheep, a key molecular marker and a practical technical scheme are provided for molecular assisted breeding of F17 escherichia coli diarrhea resistance of the sheep, and the occurrence risk of diarrhea diseases in the breeding industry can be reduced from the heredity essence.

B cell conservative epitope peptide of campylobacter jejuni MapA protein and application of B cell conservative epitope peptide in detection

Resumen de: CN122464966A

The invention discloses a B cell conservative epitope peptide of a campylobacter jejuni MapA protein and application of the B cell conservative epitope peptide in detection. According to the invention, an LP9 coding gene is inserted into a Peg pilus operon gene, and is introduced into an S9H inert vector bacterium, so that a recombinant strain capable of functionally exhibiting and expressing an LP9 epitope on the surface of a thallus is obtained. The recombinant strain can specifically recognize and combine with an antibody generated by campylobacter jejuni infection or immunization, and has no cross reaction with healthy serum and other positive serum of enteropathogenic bacteria. The LP9 epitope directly mediated antibody agglutination detection system constructed on the basis of the recombinant strain can detect a specific antibody 3 days after a mouse is infected with campylobacter jejuni, is rapid and convenient, and is expected to become a technology and platform for campylobacter jejuni infection diagnosis, on-site screening and monitoring.

Listeria monocytogenes nucleic acid aptamer combination with high affinity and specificity and application thereof

Resumen de: CN122445650A

The invention discloses a listeria monocytogenes nucleic acid aptamer combination with high affinity and specificity and application of the listeria monocytogenes nucleic acid aptamer combination. The nucleic acid aptamer combination obtained by research contains three specific aptamers, and the combination has multiple recognition sites and can synergistically act on specific detection of Listeria monocytogenes, so that the affinity and sensitivity of the aptamers to target substances are effectively improved; the nucleic acid aptamer is further used for preparing a biosensor, and the nucleic acid aptamer combination is modified on the sensor, so that the capturing efficiency of viable bacteria cells can be effectively improved, and the sensitivity and the detection accuracy are further improved. The optimized nucleic acid aptamer combination has high affinity and specificity and is used as an important recognition element to realize high-sensitivity detection on the listeria monocytogenes in a CNT-FET sensing platform, and the accuracy reaches 100%; and the method can also be combined with other sensing platforms to show superiority and huge potential in the aspect of efficiently diagnosing the food-borne bacteria.

Kit for rapidly detecting 15 respiratory tract pathogens and use method thereof

Resumen de: CN122445825A

The invention relates to the technical field of microfluidics, in particular to a kit for rapidly detecting 15 respiratory tract pathogens and a use method of the kit. Comprising a plurality of reaction cavities, and primer groups corresponding to 15 respiratory tract pathogens are arranged in the reaction cavities respectively; the primer group comprises mycoplasma pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, klebsiella pneumoniae, stenotrophomonas maltophilia, haemophilus influenzae, streptococcus pneumoniae, escherichia coli, legionella pneumophila, burkholderia, staphylococcus aureus, corynebacterium striatum, aspergillus and pneumocystis yarrowii. The inner primer, the outer primer and the loop primer are used for PCR amplification of candida albicans. According to the invention, 15 respiratory tract pathogens can be detected at the same time through one-time sample adding, liquid reagents are prevented from being added into a plurality of detection tubes, and the manual operation intensity and the possible mismatching risk are reduced.

Rapid detection kit for third-generation cephalosporin-resistant escherichia coli ST131 and application of rapid detection kit

Resumen de: CN122445827A

The invention discloses a rapid detection kit for third-generation cephalosporin-resistant escherichia coli ST131 and application of the rapid detection kit, and belongs to the field of molecular biology. The invention combines an RPA isothermal amplification technology and a Cas12a/Cas13a detection technology, provides a rapid detection method for a specific fimH30 adhesin allele and a blaCTX-M drug-resistant gene of an escherichia coli ST131 pathogenic strain in a clinical sample, and realizes specific, convenient and accurate detection of pathogenic and drug-resistant escherichia coli ST131. Under the condition of constant temperature, the detection can be completed in only one hour, the sensitivity is similar to that of a qPCR method, meanwhile, the method has extremely high accuracy and convenience, complex equipment and professional operators are not needed, and on-site instant detection can be completed only through one constant temperature instrument and portable fluorescence detection equipment.

Method, composition and kit for rapidly detecting pseudomonas syringae

Nº publicación: CN122445830A 24/07/2026

Solicitante:

CHINA ACAD OF QUALITY INSPECTION & TESTING SCIENCES
\u4E2D\u56FD\u8D28\u91CF\u68C0\u9A8C\u68C0\u6D4B\u79D1\u5B66\u7814\u7A76\u9662

Resumen de: CN122445830A

The invention relates to an oligonucleotide primer probe composition for detecting vegetable leaf spot pathogen pseudomonas syringae based on an ERA fluorescence method or an ERA test strip method, and a detection method using the composition. The method can be used for simply, quickly, specifically, sensitively and visually identifying various pathogenic variants of the vegetable leaf spot disease pathogen pseudomonas syringae in a broad-spectrum manner, is simple and convenient to operate and short in reaction time, does not need complex temperature control equipment, and can be widely applied to on-site quick screening and disease diagnosis of the pseudomonas syringae in vegetable samples.