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68
resultados
Última actualización
02/08/2026 [07:34:00]
Resumen de: US20260218268A1
Disclosed are nucleic acid oligomers, including amplification oligomers and detection probes, for detection of Yersinia enterocolitica, Vibrio spp., Shiga Toxin Expressing E. coli (STEC) O157, and Plesiomonas shigelloides target nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding formulations, reaction mixtures, and kits. Methods of synthesizing the nucleic acid oligomers are also disclosed.
Resumen de: EP4772522A2
Disclosed herein are methods and compositions for detecting one or more pathogens that cause atypical pneumonia. Detectable pathogens include Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophila.
Resumen de: US20260168026A1
The present invention relates to a CRISPR-Cas-based composition for detection of Listeria monocytogenes and a Listeria monocytogenes detection method using same and, more specifically, to a composition for detection of Listeria monocytogenes, comprising a primer pair capable of specifically amplifying Listeria monocytogenes by isothermal amplification, a guide RNA, and a CRISPR-Cas protein, and a detection method using same.
Resumen de: WO2026121311A1
The present invention provides: a method for detecting an antibody against colibactin-producing bacteria in a subject; and a reagent or kit for detecting an antibody against colibactin-producing bacteria in a subject. Provided is a method for detecting an anti-colibactin-producing bacteria antibody in a sample, said method including a step for bringing the sample into contact with at least one of the lipopolysaccharides from among O2, O4, O6, O18, and O50 serotypes of Escherichia coli. Provided is a reagent or kit for detecting an anti-colibactin-producing bacteria antibody, said reagent or kit containing a sample and at least one of the lipopolysaccharides from among O2, O4, O6, O18, and O50 serotypes of Escherichia coli.
Resumen de: CN122104968A
The invention relates to a multiple digital PCR detection reagent, a detection device and a detection method for food-borne pathogenic bacteria. The invention provides an ultra-multiple food-borne pathogenic bacterium digital PCR (Polymerase Chain Reaction) detection system based on a multicolor coding technology. The system breaks through the physical limitation of a fluorescence channel through an innovative probe coding strategy and a signal decoding algorithm, realizes simultaneous absolute quantitative detection of seven or more food-borne pathogenic bacteria in a single reaction tube, and ensures that the system still has high specificity, high accuracy, wide linear range and good repeatability in a complex matrix.
Resumen de: CN122104859A
The invention relates to the technical field of microbiological detection, in particular to a kit and a method for detecting clostridium perfringens on site. The method comprises the following steps: putting a to-be-detected sample into a sample dilution tube for dilution and standing, dipping a sampling cotton swab into supernatant liquid, then vertically inserting the sampling cotton swab into a detection tube, retaining a cotton swab rod in the tube, and standing and recondensing to form a dual anaerobic sealing system; selecting room-temperature culture or constant-temperature culture at 37 DEG C for 2-6 hours according to the environment temperature; and observing the blackening condition of the culture medium to judge that blackening becomes positive and no blackening becomes negative. According to the method, professional equipment such as an anaerobic incubator and a sterile operation table is not needed, operation is simplified, complex pretreatment is not needed, the result can be rapidly judged and read within 2-6 h, full-process detection can be completed on sites such as farms and outdoors without laboratory conditions, and the problems that an existing detection method is high in equipment dependency degree, tedious in operation, long in period, insufficient in anaerobic guarantee and the like are effectively solved; the method is suitable for base-level and on-site rapid screening application.
Resumen de: US20260146994A1
A system and method for detecting, enumerating, or extracting microorganisms in a sample is disclosed. Target microorganisms, such as Salmonella bacteria, may be of interest. Magnetic beads may be bound to the target microorganisms. After which, the bead-bound cells may be isolated. For example, a magnetic field may be applied in order to separate the target cells (with the magnetic beads attached thereto) and move then to a predetermined section of the well. Agar, or other immobilizing agent, may be added to the wells in order to immobilize the target cells. After which, the target cells are incubated and periodically analyzed to determine whether the target cells are growing, thereby indicating that the microorganisms are contained within the well.
Resumen de: CN122081521A
The invention belongs to the technical field of molecular biological detection, and particularly relates to a dual fluorescent quantitative PCR primer and probe for detecting isospora suis and enterotoxigenic escherichia coli and application of the dual fluorescent quantitative PCR primer and probe. The invention discloses a dual fluorescent quantitative PCR (Polymerase Chain Reaction) primer and a probe. The dual fluorescent quantitative PCR primer comprises an upstream primer ITS1-F, a downstream primer ITS1-R, a probe ITS1-Probe, an upstream primer ST-F, a downstream primer ST-R and a probe ST-Probe, wherein the upstream primer ITS1-F is shown as SEQ ID NO.1, the downstream primer ITS1-R is shown as SEQ ID NO.2, the probe ITS1-Probe is shown as SEQ ID NO.3, the upstream primer ST-F is shown as SEQ ID NO.4, the downstream primer ST-R is shown as SEQ ID NO.5, and the probe ST-Probe is shown as SEQ ID NO.6, and the upstream primer ST-F is shown as SEQ ID NO.4, the downstream primer ST-R is shown as SEQ ID NO.6. The dual fluorescent quantitative PCR primer and probe provided by the invention have the advantages of high sensitivity, high specificity and good repeatability, can synchronously detect isospora suis and enterotoxigenic escherichia coli, and can improve the diagnosis efficiency.
Resumen de: CN122081523A
The invention belongs to the technical field of biological detection, and particularly relates to a high-sensitivity and high-specificity quantitative detection method for total RNA (ribonucleic acid) of Escherichia coli remained in the production process of biological products (such as recombinant proteins, vaccines, cell therapy products and the like). Particularly relates to detection system construction, primer probe design and standardized operation processes based on a reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) technology, and further expands application of the detection system construction, primer probe design and standardized operation processes in drug quality control, process verification and cleaning verification.
Resumen de: CN122081528A
The invention belongs to the technical field of molecular detection, and particularly relates to a detection kit for respiratory tract pathogenic bacteria and drug-resistant genes based on a capillary electrophoresis detection platform and application of the detection kit. Compared with a traditional method, the method has the advantages that common respiratory tract infection pathogenic bacteria and drug-resistant genes are covered, detection sites are more comprehensive, specificity is higher, sensitivity is better, reliability is higher, and batch detection capacity is achieved.
Resumen de: CN122081526A
The invention belongs to the technical field of molecular biological detection, and particularly relates to a primer and probe combination for detecting avian salmonella based on an RAA-LFD method and application of the primer and probe combination. The invention particularly relates to a primer and probe combination for detecting three avian serotype salmonella such as salmonella pullorum, salmonella enteritidis and salmonella typhimurium based on an RAA-LFD method and application of the primer and probe combination. Different types of probes and primers are respectively designed aiming at genes of serotype salmonella SP, SE and STM, rapid, sensitive and specific detection of salmonella and three serotype avian salmonella such as SP, SE and STM can be realized by adopting the primer and probe combination, the detection period of avian salmonellosis is greatly shortened, the detection time is shortened, and the detection efficiency is improved. The detection efficiency and accuracy of the avian salmonellosis are improved.
Resumen de: CN122081529A
The invention discloses a composition for simultaneously detecting staphylococcus aureus and salmonella based on CRISPR-Cas12a/Cas12b, a method and application, and belongs to the technical field of food safety detection. Based on the characteristics of RPA isothermal amplification and preference of different Cas12 enzyme cleavage positions, a dual RPA-CRISPR-Cas12a/Cas12b detection method for staphylococcus aureus and salmonella is established through primer screening and optimization of parameters such as crRNA, reaction time and temperature, and the problem that it is difficult to detect multiple DNA targets of a Cas12 system at the same time is effectively solved. The method is good in specificity, high in sensitivity and simple in result interpretation, the detection limits of pure cultures and artificially polluted samples can reach the order of magnitude of 100 CFU/mL, detection can be completed within 1 h, and a new technical path is provided for rapid screening of food-borne pathogenic bacteria.
Resumen de: CN122095108A
Described herein are compositions, methods, and kits for detecting diarrhea-causing pathogens from a patient, food, or environmental sample. One embodiment described herein is a primer pair and probe for a multiplex polymerase chain reaction (PCR)-based assay for the detection of gastrointestinal pathogens, these gastrointestinal pathogens such as adenovirus, astrovirus, rotavirus, Norovirus, fiveleaf virus, Campylobacter strain, Clostridium difficile, Enteroaggregating Escherichia coli (EAEC), Enteropathogenic Escherichia coli (EPEC), Enterotoxigenic Escherichia coli (ETEC), Shiga-like toxin producing Escherichia coli (STEC), Shigella/Enteroinvasive Escherichia coli (EIEC), Escherichia coli O157, Escherichia coli O157, Escherichia coli O157, Escherichia coli O157, Escherichia coli O157, Escherichia coli O157, Escherichia The composition is prepared from the following raw materials: bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, listeria monocytogenes, plesiomonas shigelloides, salmonella strains, yersinia enterocolitica, vibrio strains, cryptosporidium strains, banquette sporidium, entamoeba histolytica, giardia intestinal or giardia blue. Other embodiments include methods and kits for detecting gastrointestinal pathogens.
Resumen de: CN122084896A
The invention discloses a rapid screening and quantitative detection method for salmonella in a grease feed additive, and relates to the technical field of feed additive detection, the detection method comprises the following steps: matrix compatible pretreatment; selective affinity enrichment: a rapid screening channel and a quantitative channel; and the inner part participates in matrix response calibration. Through matrix compatible pretreatment of reversible lipid shielding, interface interference of grease on a capture layer and a detection reaction is reduced, and the problems of antibody ligand site shielding, reagent adsorption, nuclease reaction inhibition and the like caused by free grease in a high-grease matrix are solved; target mark damage or detection failure caused by pure strong chemical degreasing is avoided, the downstream capture and detection sensitivity is remarkably improved on the premise of not damaging target recognition sites, the sample compatibility is improved, the field pretreatment complexity is shortened, and rapid quality inspection process landing is facilitated.
Resumen de: CN122060931A
The invention provides a kit for detecting multiple piglet diarrhea pathogens and a detection method, and belongs to the technical field of biological information detection. The kit comprises a reaction reagent, wherein the reaction reagent comprises a porcine epidemic diarrhea virus specific primer and a probe, a porcine delta coronavirus specific primer and a probe, a piglet pathogenic escherichia coli specific primer and a probe, a clostridium perfringens specific primer and a probe, a buffer solution, an enzyme mixed solution and a freeze-drying protective additive. The detection method adopts the kit for detecting various piglet diarrhea pathogens. According to the kit disclosed by the invention, simultaneous detection of four target genes including the porcine epidemic diarrhea virus, the porcine delta coronavirus, the piglet pathogenic escherichia coli and the clostridium perfringens can be realized only through one-time amplification, so that the detection time is greatly shortened while the detection cost is reduced; the kit has extremely high specificity, sensitivity and repeatability, and the primers and the probes do not interfere with each other; good normal-temperature storage stability is also realized.
Resumen de: CN122060834A
The invention discloses a rapid and accurate detection method for pathogenic bacteria. The method comprises the following steps: immobilizing bacteriophages on the surfaces of magnetic nanoparticles through physical adsorption or chemical reaction, selectively enriching pathogenic bacteria in a complex sample by adopting the bacteriophage functionalized magnetic nanoparticles, quickly cracking the pathogenic bacteria on the surfaces of the magnetic nanoparticles by using CTAB (cetyltrimethyl ammonium bromide), releasing ATP in the bacteria, and quantitatively analyzing ATP by using bioluminescence. Further calculating the content of the pathogenic bacteria; the detection method is free of enrichment and amplification, can complete capture, enrichment and detection of pathogenic bacteria within 20 minutes, and can specifically detect viable bacteria of target bacteria in various complex matrix (such as food) samples due to the fact that bacteriophages are adopted as recognition molecules.
Resumen de: CN122060843A
The invention discloses a pathogenic bacteria splitting decomposition and hydrogel in-situ detection method based on an Au-TiO2 coating, and belongs to the technical field of biology. The method comprises the following steps: (1) preparing an Au-TiO2 composite coating on a substrate; (2) dropwise adding a to-be-detected liquid sample to the surface of the Au-TiO2 composite coating, and covering the hydrogel sheet after the solvent is evaporated; or adhering the hydrogel sheet to the surface of the solid sample to be detected, stripping the adsorbed pathogenic bacteria, and covering the Au-TiO2 composite coating with the hydrogel sheet; the hydrogel sheet contains lysozyme, an isothermal amplification reaction reagent and a fluorescent dye; and (3) incubating the reaction system under an isothermal amplification reaction condition, and carrying out fluorescence imaging after the reaction is finished. According to the method provided by the invention, through hydrogel fixation and synergistic effect of lysozyme and Au-TiO2, splitting decomposition of pathogenic bacteria on an interface layer is promoted, in-situ nucleic acid amplification is realized, and the method has an extremely high application value for on-site rapid detection of pathogenic bacteria of agricultural products.
Resumen de: CN122060881A
The invention belongs to the technical field of microbiological detection and molecular diagnosis, and discloses a primer group, a kit and a detection method for detecting salmonella enteritidis, the kit comprises an immunocapture component, an isothermal amplification component, a CRISPR/Cas12a detection component and auxiliary components; the isothermal amplification component is any one of an RPA (recombinase polymerase amplification) component or an LAMP (loop-mediated isothermal amplification) component, the RPA component comprises a specific primer group targeting 86th to 212th nucleotides in a salmonella enteritidis hilA gene coding region, and the LAMP component comprises a specific primer group targeting 58th to 250th nucleotides in the salmonella enteritidis hilA gene coding region. The primer group, the kit and the detection method for detecting the salmonella enteritidis successfully integrate three key technologies of RAP and LAMP rapid isothermal amplification, CRISPR/Cas12a high-specificity recognition and trans-cleavage and immunomagnetic bead targeted enrichment, and creatively provide the primer group, the kit and the detection method for detecting the salmonella enteritidis.
Resumen de: CN122060880A
The invention discloses a persistent listeria monocytogenes specific molecular target and a rapid detection method thereof, and belongs to the technical field of microbiological detection. The molecular target is obtained through bioinformatics analysis and screening, is further screened through specific experimental verification, and is respectively specific genome sequence fragments of six retention types of listeria monocytogenes CC5, CC8, CC31, CC87, CC121 and CC204. A corresponding specific primer group is designed based on the target, sample DNA is amplified through PCR, a product is subjected to visual analysis in combination with gel electrophoresis, and whether the target retention type strain exists or not can be judged according to the size of a band. The detection method has the advantages of being short in detection time, low in cost, simple and convenient to operate and high in specificity, results are accurate, judgment is easy, practicability is high, the limitation that traditional culture and identification periods are long, and phenotypic analysis steps are tedious is broken through, and the detection method is suitable for rapid screening of the persistent listeria monocytogenes in samples of food, environment and the like.
Resumen de: CN122038409A
The invention discloses a biosensor detection reagent for detecting salmonella typhimurium and a preparation method, and belongs to the technical field of biomedicine. According to the invention, a DNA sequence of an amino-modified nucleic acid aptamer for detecting salmonella typhimurium is screened out and is shown as SEQ ID No: 1. Based on the nucleic acid aptamer, amino modified aptamer-fluorescent nano-enzyme serving as an energy donor and sulfydryl modified aptamer-gold nanoparticles serving as an energy receptor are mixed according to the volume ratio of 1: 1, and the biosensor detection reagent is prepared. The linear detection range of the biosensor detection reagent on salmonella typhimurium is 101-107 CFU/mL, the linear correlation coefficient R2 is equal to 0.9981, and the detection limit is as low as 1.527 CFU/mL. The biosensor detection reagent disclosed by the invention still keeps good selectivity and stability in a complex environment with high protein, high fat and high salt, and is suitable for rapid field detection in the fields of food safety and medical diagnosis.
Resumen de: CN122038605A
The invention relates to a primer probe group, a multiplex fluorescent quantitative PCR method and a kit for simultaneously detecting six pathogenic bacteria, and belongs to the technical field of molecular biology, the primer probe group comprises primer sequences and probe sequences shown in SEQ ID No.1-18 in a table 1; the 5'end of each probe sequence is modified with a reporter group, and the 3 'end of each probe sequence is modified with a quenching group; the reporter group is Cy5, FAM and VIC, and the quenching group is Eclipse. The invention provides a method for simultaneously detecting six pathogenic bacteria including vibrio parahaemolyticus, salmonella, staphylococcus aureus, escherichia coli O157: H7, listeria monocytogenes and shigella in the same reaction system by combining a multiple real-time fluorescent quantitative PCR (Polymerase Chain Reaction) technology. The multiplex real-time fluorescent quantitative PCR kit provided by the invention is used for detecting the six pathogenic bacteria, can improve the detection sensitivity and specificity, greatly improves the detection efficiency, and overcomes the defects of complex steps, long detection period and incapability of adapting to large-scale rapid detection in the conventional detection method.
Resumen de: CN122038620A
The invention belongs to the field of biomedical treatment, and particularly relates to a convective amplification primer pair and probe for respiratory tract pathogenic bacteria and multiple detection application of the convective amplification primer pair and probe. The convection amplification primer pair comprises a legionella pneumophila primer pair, a mycoplasma pneumoniae primer pair and a bordetella pertussis primer pair, and the legionella pneumophila primer pair, the mycoplasma pneumoniae primer pair and the bordetella pertussis primer pair are respectively composed of an upstream primer and a downstream primer; each of the upstream primer and the downstream primer comprises a target nucleic acid complementary sequence and an interface sequence, and the interface sequence is located at the 5'end of the target nucleic acid complementary sequence and is not complementary with target nucleic acid; the target nucleic acid complementary sequence has a folded sequence, and the folded sequence is complementary with the interface sequence, so that the primer sequence has a curved hairpin structure, a probe is designed, and a respiratory tract pathogenic bacteria multiple detection kit is prepared. The multiple detection can avoid non-specific amplification and improve the detection sensitivity.
Resumen de: CN122038621A
The invention belongs to the field of biomedical treatment, and particularly relates to a convection amplification primer group, a probe and a kit for multiple detection of lower respiratory pathogens. The convection amplification primer pair comprises a legionella pneumophila primer pair, an adenovirus primer pair and a chlamydia psittaci primer pair, primer sequences in the legionella pneumophila primer pair, the adenovirus primer pair and the chlamydia psittaci primer pair respectively comprise a target nucleic acid complementary sequence and an interface sequence, and the interface sequence is located at the 5'end of the target nucleic acid sequence; the nucleic acid is not complementary with the target nucleic acid; the target nucleic acid complementary sequence has a folded sequence, and the folded sequence is complementary to the interface sequence, so that the primer sequence has a bent hairpin structure. Meanwhile, a primer probe is designed, and a respiratory tract pathogenic bacteria multiple detection kit is prepared. The multiple detection can avoid non-specific amplification and improve the detection sensitivity.
Resumen de: CN122038544A
The invention belongs to the technical field of environmental biological safety monitoring, and particularly relates to an RPA-CRISPR method for rapidly detecting pathogens in air. According to the invention, a two-step detection strategy integrating RPA isothermal amplification and CRISPR detection is established; the method specifically comprises the following steps: firstly, acquiring genome DNA of an air sample, and carrying out specific amplification on a trace target by utilizing an RPA technology; then, a product is introduced into a CRISPR-Cas12a system, and the trans-shear activity is recognized and activated by utilizing the specificity of crRNA, so that signal cascade amplification is realized. Pseudomonas aeruginosa is used as a model pathogen, and a primer and probe combination is designed aiming at a specific gene of the pseudomonas aeruginosa. Results show that the method can complete detection within 10-15 minutes at the soonest, the lowest detection limit can reach a single copy level (100 copies/L), and the method has the advantages of high environmental interference resistance, high sensitivity and no need of large instruments, and can meet the on-site rapid screening requirements of air pathogen aerosol in public places, medical environments and other scenes.
Nº publicación: CN122042964A 15/05/2026
Solicitante:
ZHEJIANG JUNENG CO LTD
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Resumen de: CN122042964A
The invention relates to a method for detecting multiple pathogenic bacteria by using magnetic separation time difference, and belongs to the technical field of microbiological analysis and detection. According to the invention, the dual-mode aptamer sensor is integrated, so that rapid and accurate multiple pathogenic bacteria detection can be realized. The core of the time separation system based on magnetic coding comprises fluorescent magnetic coding nanoparticles, a gold nanoparticle-aptamer and a fluorescent-ultraviolet dual-mode probe. A preparation process of the fluorescent-ultraviolet dual-mode probe specifically comprises the following steps: sequentially anchoring quantum dots and specific cDNA on the surface of a magnetic coding nanosphere; and carrying out functional combination with aptamer-modified gold nanoparticles to finally synthesize the dual-mode probe with both fluorescence signal response and ultraviolet absorption response. The probe can realize targeted recognition and separation detection of various pathogenic bacteria through specific binding of the surface aptamer and target pathogenic bacteria.
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