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Detection system for food-borne pathogenic bacteria and application thereof

Resumen de: CN122405789A

The invention belongs to the technical field of biological detection, and particularly relates to a detection system for food-borne pathogenic bacteria and application of the detection system. The detection system for the food-borne pathogenic bacteria comprises a composite response material, hyaluronic acid hydrogel containing L-ascorbic acid-2-trisodium phosphate and bacteriophage, the composite response material is prepared from ZIF-90 nano particles, hyaluronidase and alkaline phosphatase, wherein the hyaluronidase and the alkaline phosphatase are encapsulated in the ZIF-90 nano particles. According to the invention, a dual-mode paper-based visual detection system based on combination of phage specific recognition and a ZIF-90 response release mechanism is constructed, and high-specificity detection of target pathogenic bacteria is realized. Compared with a nucleic acid amplification or immunological method which has the defect that live bacteria and dead bacteria are difficult to distinguish, the method provided by the invention only generates signal response to the live bacteria which can be infected by the bacteriophage, so that the authenticity and the reliability of a detection result are remarkably improved.

Specific primer combination for detecting glacier melt water transmission related pathogenic microorganisms

Resumen de: CN122405852A

The invention relates to a specific primer combination for detecting glacier melt water transmission related pathogenic microorganisms. The specific primer combination comprises a vibrio cholerae primer Seq1 with a target gene of ompW, a bacillus cereus primer Seq4 with a target gene of gyrB, a clostridium botulinum primer Seq7 with a target gene of bond/A, and a legionella pneumophila primer Seq10 with a target gene of mip. The method is high in sensitivity and good in environmental applicability, not only can realize joint detection of spreading related pathogenic microorganisms in the glacier environment, but also can be used for analyzing migration and diffusion paths in different environmental media, and provides technical support for glacier melt water spreading process and downstream biological safety risk assessment.

Salmonella dual-mode detection method based on medium-temperature Argonaute driving

Resumen de: CN122409630A

The invention discloses a salmonella dual-mode detection method based on medium-temperature Argonaute driving, and belongs to the technical field of food safety detection. According to the method, the functionalized DNA hydrogel internally packaged with an asymmetric RPA system, a CbAgo system and nano-enzyme is constructed, cascade signal amplification, DNA hydrogel phase change and nano-enzyme signal output links are integrated, and rapid detection of salmonella is realized under the assistance of gravity. The invention also independently develops portable detection equipment and an intelligent application program, realizes automatic image acquisition and analysis, has the advantages of high sensitivity, strong specificity and simplicity and convenience in operation, and is suitable for on-site rapid detection of food safety.

Monoclonal antibody for detecting surface protein SapA of campylobacter fetus and application of monoclonal antibody

Resumen de: CN122404548A

The invention discloses a monoclonal antibody for detecting surface protein SapA of campylobacter fetus and application of the monoclonal antibody. The monoclonal antibodies are named as 4F6 and 7B3 respectively, and amino acid sequences of variable regions of heavy chains and light chains of the two antibodies are disclosed. The monoclonal antibody prepared by the invention can specifically recognize the surface protein SapA of the campylobacter fetus, has better specificity, and does not have cross reaction with non-campylobacter such as standard escherichia coli, salmonella, listeria, klebsiella pneumoniae, staphylococcus aureus, streptococcus and the like; no cross reaction with campylobacter jejuni, campylobacter colon and other campylobacter is generated; the test strip prepared by combining the monoclonal antibody and colloidal gold can realize on-site rapid detection of the campylobacter fetus, the detection limit is low, the sensitivity is high, and an immunological method capable of specifically detecting the campylobacter fetus is successfully developed.

Method, composition and kit for rapidly detecting two food-borne pathogenic pseudomonas

Nº publicación: CN122405858A 17/07/2026

Solicitante:

CHINA ACAD OF QUALITY INSPECTION & TESTING SCIENCES
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Resumen de: CN122405858A

The invention relates to an oligonucleotide primer probe composition for detecting food-borne pathogenic pseudomonas bacteria (pseudomonas alcaligenes and pseudomonas buchneri) based on an ERA fluorescence method or an ERA test strip method, and a detection method using the composition. The pseudomonas alcaligenes or pseudomonas buchneri in the sample can be rapidly, specifically and sensitively detected, and the method is easy and convenient to operate, short in reaction time and free of complex temperature control equipment, and can be widely applied to rapid screening, pollution monitoring and risk early warning of food-borne pathogenic pseudomonas in various food samples such as milk and dairy products.