Alerta

GLUCOAMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME

Resumen de: US2025236859A1

The present invention relates to glucoamylase variants having improved thermostability. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

GLUCOAMYLASE VARIANTS AND METHODS FOR USE THEREOF

Resumen de: US2025236858A1

Described herein, inter cilia, are glucoamylase variants and methods of using the same for saccharifying a starch substrate. Moreover, the disclosure also relates to a process of producing fermentation products and a method for increasing starch digestibility in an animal as well as a method of producing a fermented beverage using said as well as a. method of producing a fermented beverage using said glucoamylase variants.

METHOD FOR SEPARATING BIOMASS FROM SOLID FERMENTATION PRODUCT

Resumen de: US2025236836A1

The invention pertains to a method for separating biomass from solid fermentation product wherein a slurry comprising biomass and solid fermentation product is provided to the top of a biomass separator unit and an aqueous medium is provided to the bottom of a biomass separator unit, while a product stream comprising solid fermentation product is withdrawn from the bottom of the biomass separator unit and a waste stream comprising biomass is withdrawn from the top of the biomass separator unit. It has been found that the method according to the invention makes it possible to provide a solid fermentation product which comprises very low amounts of remaining biomass, while product loss can be limited.

YEAST EXPRESSING SACCHAROLYTIC ENZYMES FOR CONSOLIDATED BIOPROCESSING USING STARCH AND CELLULOSE

Resumen de: US2025236875A1

The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

USE OF MUTANT GENE OF ZMNST2 AND MUTANT THEREOF IN PRODUCTION OF BIOETHANOL BY MEANS OF CORN STOVER FERMENTATION

Resumen de: WO2025152404A1

The present invention relates to the technical field of genetic engineering and breeding. Provided is the use of a mutant gene of ZmNST2 and a mutant thereof in the production of bioethanol by means of corn stover fermentation. The mutant gene of ZmNST2 is obtained via molecular genetic means. Additionally, by means of the determination of lignin content and ethanol fermentation level, it is found that the mutant gene of ZmNST2 is capable of reducing the lignin content in corn, reducing the content of fermentation inhibitors and improving the hydrolysis rate of cellulase, such that the yield and efficiency in the production of bioethanol by means of corn stover fermentation can be improved. The provided mutant gene of ZmNST2 plays a critical role in the use of same in the production of bioethanol by means of corn stover fermentation, and is an important resource for cultivating new corn varieties that have high ethanol yield by means of fermentation.

BIOMASS FEEDSTOCK RECOVERY EQUIPMENT AND PROCESSES

Resumen de: US2025235874A1

The present disclosure is broadly concerned with processing equipment and methods for processing of a biomass feedstock into one or more biomass particles of reduced size, and in particular, hemp. The vertical impact liberating equipment and methods can process whole hemp stalks into hemp particulates comprising distinct fractions of hemp hurds, bast fiber, and fines. Advantageously, in preferred embodiments, the biomass feedstock is pulled through a defined gap and into a defined chamber. This action causes a successive decrease and increase of pressure within the biomass feedstock, yielding the one or more biomass particles of reduced size.

USE OF LACTIC ACID BACTERIA FOR PREPARING FERMENTED FOOD PRODUCTS WITH INCREASED NATURAL SWEETNESS

Nº publicación: US2025234877A1 24/07/2025

Solicitante:

CHR HANSEN AS [DK]
CHR. HANSEN A/S

Resumen de: US2025234877A1

The dairy industry today faces a problem of providing an alternative to adding sweeteners to fermented milk products in order to achieve the desired sweet taste without the added calories. Furthermore, it would be highly advantageous to establish a method for reducing lactose in fermented milk products to a level which is acceptable for lactose-intolerant consumers. The above problems have been solved by providing mutant Streptococcus thermophilus strains and mutant Lactobacillus delbrueckii subsp. bulgaricus strains that excrete glucose to the milk when the milk is inoculated and fermented with such Streptococcus thermophilus strains and Lactobacillus delbrueckii subsp. bulgaricus strains.Thus, the present invention relates to strains of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus which secrete glucose to the milk substrate during fermentation, as well as to mixed cultures comprising the Streptococcus thermophilus strains and the Lactobacillus delbrueckii subsp. bulgaricus strains, starter cultures comprising the strains and dairy products manufactured with the cultures. The present method also relates to use of the strains for decreasing the lactose content of a fermented food product and for boosting growth of the probiotic BB-12®.