Salmonella

InDel MOLECULAR MARKER OF hisD GENE OF ULTRASONICALLY-MUTAGENIZED SALMONELLA TYPHIMURIUM AND USE THEREOF

Resumen de: US2024263250A1

An insertion and deletion (InDel) molecular marker of a hisD gene of ultrasonically-mutagenized Salmonella typhimurium (S. typhimurium) and use thereof are provided. Through ultrasonic mutagenesis to S. typhimurium and sequencing, it is found that all InDel mutations occur in a core mutation region of the hisD gene. Therefore, the core mutation region is used as the InDel molecular marker to determine an insertion or a deletion of a gene reverse mutation, and the InDel molecular marker can also be used to analyze a relationship between a sequence of the hisD gene and a function of a protein encoded thereby.

一种陶瓷抗细菌性能的快检方法

Resumen de: CN110272962A

The invention relates to a method for detecting the bacterium resisting properties of bacterium resisting ceramics. The method comprises the detection steps of preparing samples and performing pretreatment: performing type selection of equipment, and compounding a reagent and culture mediums; performing strain preservation, performing strain activation and preparing bacterium suspension; establishing a standard curve of the ATP concentration logarithm value lgC<A>-relative fluorescence intensity logarithm value lgI<A> , and performing calibration on the ATP concentration C<A> of viable bacteria of inoculation bacterial liquid; performing sample inoculation, performing sample culture and performing eluting and recovering; determining I<A> of recovery liquid, and reckoning ATP concentration C<A> and ATP concentration T<A> of the viable bacteria; calculating bacterium resisting rate R or bacterium resisting activity value A; and performing result evaluation. The method has the characteristic that the bacterium resisting properties which are represented by R or A, of the ceramics can be accurately and quantitatively tested through an ATP fluorophotometer. Control samples and bacterium resisting samples after contact for 0h and culture for 24h are eluted and recovered according to the specification of the invention, the I<A> of the recovery liquid is determined and represented by lgI<A>, and R or A is calculated; and a result evaluat

生弱毒化細菌を用いたがん療法

Resumen de: US2024226190A1

The present invention relates to the field of cancer therapy. In particular, the present invention relates to a first composition comprising a live attenuated bacterium for use in the treatment, prevention, reduction, inhibition, prevention of recurrence, or control of a neoplastic disease in a subject being or intended to be administered a second composition comprising a live attenuated bacterium, said bacterium being the same or different to that of the first composition and methods thereof.

METHODS FOR PRODUCING HIGH-LEVEL SQUALENE

Resumen de: WO2024159003A1

Aspects of the present disclosure relate to modified yeast cells capable of producing increased quantities of squalene as compared to unmodified yeast cells and methods of using the same.

METHOD FOR IDENTIFYING A MULTI-PARAMETER PHENOTYPE OF MICROBIOTA

Resumen de: WO2024156739A1

The invention relates to a method for identifying a multi-parameter phenotype of microbiota. The method comprises (i) providing a sample comprising microbiota, (ii) labeling said microbiota with multiple labels, each of which binds a phenotypic parameter of said microbiota, (iii) detecting an intensity of the labelled phenotypic parameters of single cells of the microbiota by flow cytometry, and (iv) segmenting the single cells into bins based on the intensities of detected phenotypic parameters, wherein the distribution of single cells in bins represents a multi-parameter phenotype of said microbiota. The invention further relates to a system for identifying a multi-parameter phenotype of intestinal microbiota, a kit for identifying a multi-parameter phenotype of intestinal microbiota and methods for diagnosing a medical condition associated with microbiota, for example an inflammatory condition, such as an inflammatory bowel disease, in a subject.

Photodynamic anti-gram-positive bacterium activity of medicinal rose-bengal

Resumen de: CN118434447A

The present invention relates to the use of rose-bengal (RB) derivatives in combination with irradiation of bacteria with light to treat and kill irradiated bacteria. In one aspect, a gram-positive bacterium is treated in a method wherein the bacterium is contacted with an aqueous pharmaceutical composition containing a rose-bengal (RB) compound of Formula I as discussed herein dissolved or dispersed therein at a concentration of about 0.2 to about 3.1 mu g/mL. Those contacted bacteria are contacted with light having a wavelength of about 500 nm to about 600 nm for a period of about 1 to about 10 minutes to provide a light dose of about 0.7 to about 7.2 J/cm2. Similar methods are contemplated for the treatment of Gram-negative bacteria, which are one or more of Burkholderia, Salmonella and Proteus, with a pharmaceutical composition containing an RB compound at a concentration of from about 2 to about 15 mu M.

一种志贺氏菌鼻腔喷雾剂型疫苗智能制备系统及方法

Resumen de: CN118416208A

本发明属于疫苗制备技术领域，尤其涉及一种志贺氏菌鼻腔喷雾剂型疫苗智能制备系统及方法；智能制备系统包括：疫苗制备模块，用于获取志贺氏菌菌株的抗原，混合佐剂后得到疫苗；密封包装模块，用于灌装、封口并储存所述疫苗，并得到所述疫苗的具体信息；追踪与追溯模块，与所述疫苗制备模块和所述密封包装模块连接，用于记录和追溯所述疫苗制备过程中的详细信息。本发明通过集成先进的监控、自动化和信息技术，有效改善了现有技术的问题，提高了疫苗生产的效率、一致性和可追溯性，同时确保了疫苗的高质量和稳定性。

Broad-spectrum antibacterial peptide AG3 as well as preparation method and application thereof

Resumen de: CN118420718A

The invention provides a broad-spectrum antibacterial peptide AG3 as well as a preparation method and application thereof, and belongs to the technical field of biology, and the amino acid sequence of the broad-spectrum antibacterial peptide AG3 is shown as SEQ ID No.1. The antibacterial peptide AG3 disclosed by the invention has an obvious inhibition effect on 11 gram-negative gram-positive bacteria and fungal strains such as escherichia coli, pseudomonas aeruginosa, salmonella pullorum, staphylococcus aureus, candida albicans and the like, and the average antibacterial activity reaches 2 mu M; the bactericidal power also shows that the antibacterial agent has efficient bactericidal ability on bacteria; meanwhile, the hemolytic activity is low, and the therapeutic index is up to 32; in addition, the antibacterial peptide still has good antibacterial activity in physiological concentration salt ions, which indicates that the antibacterial peptide has high salt ion tolerance, and related mechanism tests prove that the antibacterial peptide realizes the antibacterial function through a physical membrane rupture mechanism.

RPA (recombinase polymerase amplification) primer capable of simultaneously detecting salmonella, carbapenem drug-resistant gene and virulence gene and application of RPA primer

Resumen de: CN118421814A

The invention discloses an RPA (recombinase polymerase amplification) primer capable of simultaneously detecting salmonella, carbapenem drug-resistant genes and virulence genes and application of the RPA primer. The primer designed by the invention has better interspecific specificity and intraspecific conservative property, the technology can detect salmonella, carbapenem drug-resistant genes (blaNDM) and virulence genes (PagC) in one reaction, whether the salmonella is the salmonella carrying the carbapenem drug-resistant genes (blaNDM) and the virulence genes (PagC) or not can be judged in a short time, no false positive appears, and the detection result is accurate. Therefore, the method is more convenient, efficient and accurate. The amplified fragments have different lengths and sizes, and can be separated and distinguished through electrophoresis.

RPA (recombinase polymerase amplification) primer for detecting salmonella and polymyxin drug-resistant genes or virulence genes and application of RPA primer

Resumen de: CN118421813A

The invention discloses an RPA (recombinase polymerase amplification) primer for detecting salmonella and polymyxin drug-resistant genes or virulence genes and application of the RPA primer. The primer designed by the invention has better interspecific specificity and intraspecific conservative property, the technology can detect the salmonella, the polymyxin drug-resistant gene (mcr-1) and the virulence gene (sipA) in one reaction, whether the salmonella is the salmonella carrying the polymyxin drug-resistant gene (mcr-1) and the virulence gene (sipA) or not can be judged in a short time, no false positive appears, and the detection sensitivity is high. Therefore, the method is more convenient, efficient and accurate. The amplified fragments have different lengths and sizes, and can be separated and distinguished through electrophoresis.

Nomadic bacteria and uses thereof

Resumen de: WO2023119287A1

A method of generating a biofilm of nomadic bacteria. The method comprises: (a) culturing the nomadic bacteria in an acidic environment under conditions that promote generation of a V-type structure of the nomadic bacteria; and subsequently (b) culturing said nomadic bacteria having a V-type structure on an adherent surface, thereby generating the biofilm comprising the nomadic bacteria. Conditioned media of nomadic bacteria are also disclosed and uses thereof.

A BROADLY PROTECTIVE PROPHYLACTIC VACCINE AGAINST PSEUDOMONAS AERUGINOSA

Resumen de: US2024252610A1

Disclosed are compositions comprising a fusion polypeptide comprising i) a fusion of a needle tip protein or an antigenic fragment thereof and/or a translocator protein or an antigenic fragment thereof from a Type III secretion system (T3SS) of a Gram negative bacteria and ii) the A1 subunit of the labile toxin (LTA1) from enterotoxigenic Escherichia coli or cholera toxin, and methods of their use.

COATING COMPOSITION COMPRISING BACTERIOPHAGE AND ANTIBACTERIAL FILM FORMED BY USING SAME

Resumen de: US2024254340A1

A coating composition contains a bacteriophage having a bactericidal activity against Salmonella bacteria, a polymeric compound, and a plasticizer. Thus, a coating having antibacterial activity may be prepared by using the coating composition, and the bacteriophage stably survives even after coating formation to continuously maintain excellent antibacterial activity. When the coating composition is applied to a coating or film for food packaging, it may effectively prevent food from being contaminated by Salmonella bacteria to improve food safety and shelf life.

ENGINEERED S. TYPHIMURIUM AND USES THEREOF

Resumen de: US2024252553A1

Cancer is a significant cause of mortality and morbidity worldwide. One of the principal impediments to the broad success of conventional chemotherapy is poor delivery to and transport within the tumor microenvironment (TME), caused by irregular and leaky vasculature, the lack of functional lymphatics, and underscored by the overproduction of extracellular matrix (ECM) proteins such as collagen. Described herein are engineered Salmonella Typhimurium (S. Typhimurium) bacteria and uses thereof. In some embodiments, the engineered S. Typhimurium bacteria comprises an exogenous collagenase. Also described in exemplary embodiments herein methods of using the engineered S. Typhimurium bacteria, such as part of a,cancer therapy.

LAMP (loop-mediated isothermal amplification) primer composition for detecting salmonella enteritidis as well as kit and method thereof

Resumen de: CN118406782A

The invention provides an LAMP (loop-mediated isothermal amplification) primer composition for detecting salmonella enteritidis as well as a kit and a method thereof, and belongs to the technical field of biological detection. The primer composition disclosed by the invention consists of nucleotide sequences as shown in SEQ ID NO: 1-6. The invention also provides a kit containing the primer composition and a method for detecting salmonella enteritidis. When the primer composition is used for LAMP detection of salmonella enteritidis, the sensitivity is high, the specificity is good, the accuracy rate is high, and the detection method is good in repeatability and stability. And compared with PCR, the detection effect is obviously improved. The LAMP primer composition can be used for detecting salmonella enteritidis simply and rapidly, is suitable for on-site rapid detection, and has a good application prospect.

Salmonella enteritidis determination kit and determination method thereof

Resumen de: CN118409086A

The invention relates to the technical field of analysis and detection, in particular to a salmonella enteritidis determination kit and a determination method thereof.The salmonella enteritidis determination kit comprises an R reagent and a fluorescence chromatography test strip, the R reagent is a magnetic bead-salmonella enteritidis aptamer-complementary chain-carboxyl fluorophore compound solution composed of magnetic beads, salmonella enteritidis aptamers, complementary chains and carboxyl fluorophores, and the fluorescence chromatography test strip is composed of a sample absorption pad, a combination pad, a reaction film, absorbent paper, a bottom plate and a clamping shell. The method has the characteristics of high sensitivity, good specificity, simplicity, convenience, rapidness and the like, can be used for trace detection of salmonella enteritidis, and provides a detection scheme for supervision and management of salmonella enteritidis in the fields of breeding industry, medicine and public health.

Salmonella bacteriophage vBSalPLDW8 and application thereof

Resumen de: CN118406662A

The invention belongs to the technical field of biology, and particularly relates to a salmonella bacteriophage vBSalPLDW8 and application thereof. According to the technical scheme adopted by the invention, the salmonella phage has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.45253, the phage is named as vBSalPLDW8, the nucleotide sequences of the phage are as shown in SEQ ID NO.1-SEQ ID NO.4, and the salmonella phage has broad spectrum and high splitting power on salmonella. A preparation prepared from the salmonella bacteriophage disclosed by the invention can effectively kill most salmonella infected by poultry in farms at present.

PHOTODYNAMIC ANTI-GRAM-POSITIVE BACTERIAL ACTIVITY OF PHARMACEUTICAL-GRADE ROSE BENGAL.

Resumen de: MX2024006475A

This invention contemplates combined use of a rose bengal (RB ) derivative with irradiation of bacteria with light to treat and kill the irradiated bacteria. In one aspect, Gram-positive bacteria are treated in a method in which the bacteria are contacted with an aqueous pharmaceutical composition containing a rose bengal (RB ) compound of Formula I, discussed within, dissolved or dispersed therein at about 0. 2 to about 3. 1 Î1⁄4g/mL. Those contacted bacteria are contacted with light of the wavelength about 500 nm to about 600 nm for a time period of about 1 to about 10 minutes to provide a light dose of about 0. 7 to about 7. 2 J/cm². A similar method is contemplated for treating Gram-negative bacteria that are one or more of Burkholderia, Salmonella, and Proteus using an aqueous pharmaceutical composition containing about 2 to about 15 Î1⁄4M concentration of the RB compound.

Parabacteroides disastsonis capable of inhibiting pathogenic bacteria and resisting tumors and application of parabacteroides disastsonis

Resumen de: CN118406582A

The invention discloses parabacteroides diels capable of inhibiting pathogenic bacteria and resisting tumors and application of the parabacteroides diels, relates to the technical field of microorganisms, and is technically characterized in that the preservation number of the parabacteroides diels NGMCC 1.200926 is CGMCC No: 40687, the preservation number of the parabacteroides diels NGMCC 1.200926 is CGMCC No: 40687, the preservation number of the parabacteroides diels NGMCC 1.200926 is CGMCC No: 40687, and the preservation number of the parabacteroides diels NGMCC 1.200926 is CGMCC No: 40687. The preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation unit is China General Microbiological Culture Collection Center (CGMCC); the preservation time is July 05, 2023. After the Parabacteroides dielsii NGMCC 1.2000926 disclosed by the invention is subjected to fermentation culture, a relatively strong bacteriostatic active substance can be generated, and the Parabacteroides dielsii NGMCC 1.2000926 has a relatively obvious bacteriostatic effect on pathogenic bacteria such as staphylococcus aureus, salmonella typhi, pseudomonas aeruginosa and the like, and has a very good effect of inhibiting the activity of tumor cells; the compound has a wide development space in prevention and treatment of diseases caused by pathogenic bacteria such as staphylococcus aureus, salmonella typhimurium and pseudomonas aeruginosa and tumors, and has a good

Synthesis of CDs/GO-PBA nano-composite probe and detection method of salmonella

Resumen de: CN118406489A

The invention discloses a synthesis method of a CDs/GO-PBA nano composite probe and a detection method of salmonella. The method comprises the following steps: performing hydrothermal carbonization on mannose and histidine to obtain glycopeptide mimetic carbon dots, and then reacting 3-aminophenylboronic acid with graphene oxide to obtain boric acid modified graphene oxide GO-PBA. CDs and boric acid groups form boric acid ester bonds through hydroxyl groups to be loaded on GO-PBA sheet layers, CDs/GO-PBA with quenched fluorescence is obtained, the CDs/GO-PBA is added into salmonella typhimurium bacterial suspension, after the CDs are combined with salmonella through agglutinin-sugar interaction, dynamic boric acid ester bonds are broken and separated from the surface of graphene, fluorescence is recovered, and the salmonella typhimurium is obtained. And measuring the fluorescence intensity through a fluorescence spectrophotometer so as to detect the concentration of salmonella. The method can be used for rapidly and quantitatively detecting salmonella, and is good in sensitivity and wide in detection range.

Broad-spectrum salmonella phage and application thereof

Resumen de: CN118389453A

The invention provides a broad-spectrum salmonella bacteriophage and application thereof, and belongs to the technical field of microorganisms. The phage of the broad-spectrum salmonella is named as JXAU-SP-1, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.45962 The optimal infection complex number of the bacteriophage is 0.0001, and the bacteriophage has strong acid resistance and thermal stability and is suitable for being used as a feed additive. Under the conditions that the pH value is 2-8 and the temperature is 30-60 DEG C, the phage can keep high titer. An in-vitro bacteriostasis test shows that the phage has an efficient inhibition effect on propagation of salmonella, the incubation period is 60 min, the outbreak period is 90 min, and the phage has high broad-spectrum property on salmonella. And meanwhile, the compound has a cracking effect on escherichia coli and shigella. In addition, the JXAU-SP-1 genome does not contain a virulence gene and an antibiotic resistance gene, so that the JXAU-SP-1 genome has good biological safety.

Cytokine gene delivery system as well as preparation method and application thereof

Resumen de: CN118384295A

The invention discloses a cytokine gene delivery system as well as a preparation method and application thereof, and the gene delivery system is composed of eukaryotic plasmids for driving cytokine expression, salmonella typhimurium attenuated strains VNP20009 and ZIF-8. The eukaryotic plasmid for driving the expression of the cytokines is loaded in the porous structure of the ZIF-8 to form a sphere and is attached to the surface of the salmonella typhimurium attenuated strain VNP20009. ZIF-8 containing a large number of cytokine plasmids is loaded on the surface of a VNP20009 living body, and efficient targeted delivery of cytokine genes to a tumor microenvironment is realized by utilizing the system. After macrophages endocytosis the delivery system, plasmids are released in the macrophages, expression and secretion of cytokines are started, the content of the cytokines in the tumor microenvironment can be remarkably increased, the tumor immune microenvironment can be improved, and the anti-tumor immune response can be promoted.

Cell penetrating type antibacterial peptide capable of resisting intracellular bacteria as well as preparation method and application of cell penetrating type antibacterial peptide

Resumen de: CN118388589A

The invention discloses a preparation method and application of a cell penetrating type antibacterial peptide 2L2R2W for resisting intracellular bacteria, and belongs to the technical field of biology. The amino acid sequence of the antibacterial peptide 2L2R2W is as shown in SEQ ID No. 1. The preparation method comprises the following steps: counting and analyzing the charge number, hydrophobic proportion, amino acid composition and sequence length of 1166 cell-penetrating peptides in a cell-penetrating peptide database CPPsite 2.0, and the analysis result shows that the charge number is + 5, the hydrophobic amino acid proportion is 41-60%, arginine in cationic amino acid and leucine in hydrophobic amino acid have the characteristics that the charge number is + 5, and the hydrophobic amino acid proportion is 41-60%. The occurrence frequency of the cell penetrating peptide with the amino acid residues (L) and the sequence length of 10-16 amino acid residues is the highest. In addition, tryptophan (Tryptophan, W) plays an important role in the penetrating effect of the cell penetrating peptide, so that the tryptophan is also added into the design. Based on the principles and the symmetric sequence design of the antibacterial peptide, the cell-penetrating antibacterial peptide is designed, and is synthesized by a solid-phase synthesis method. The antibacterial peptide disclosed by the invention has strong antibacterial activity on bacteria with intracellular viability, includin

DIAGNOSTIC ASSAY FOR CLASSIC INBORN GALACTOSEMIA

Resumen de: US2024247301A1

The present disclosure relates to a diagnostic assay to detect classic inborn galactosemia. The assay comprises detecting defective galactose metabolism using a substrate-specific recombinant phosphatase derived from Salmonella.

ATTENUATED SALMONELLA GALLINARUM EXPRESSING FLIC OR FLIC-HIL2 AND USE THEREOF

Nº publicación: WO2024155027A1 25/07/2024

Solicitante:

ODYSSEUS BIO CO LTD [KR]
\uC8FC\uC2DD\uD68C\uC0AC \uC624\uB514\uC138\uC6B0\uC2A4\uBC14\uC774\uC624

Resumen de: WO2024155027A1

The present invention relates to an attenuated Salmonella gallinarum expressing FliC or FliC-hiL2 and use thereof. The Salmonella strain according to the present invention has excellent immune activity and exhibits excellent anti-cancer efficacy, and thus can be used as a therapeutic agent for cancer together with or independently of existing anti-cancer drugs.