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Au-TiO2 coating-based pathogenic bacteria splitting decomposition and hydrogel in-situ detection method

Resumen de: CN122060843A

The invention discloses a pathogenic bacteria splitting decomposition and hydrogel in-situ detection method based on an Au-TiO2 coating, and belongs to the technical field of biology. The method comprises the following steps: (1) preparing an Au-TiO2 composite coating on a substrate; (2) dropwise adding a to-be-detected liquid sample to the surface of the Au-TiO2 composite coating, and covering the hydrogel sheet after the solvent is evaporated; or adhering the hydrogel sheet to the surface of the solid sample to be detected, stripping the adsorbed pathogenic bacteria, and covering the Au-TiO2 composite coating with the hydrogel sheet; the hydrogel sheet contains lysozyme, an isothermal amplification reaction reagent and a fluorescent dye; and (3) incubating the reaction system under an isothermal amplification reaction condition, and carrying out fluorescence imaging after the reaction is finished. According to the method provided by the invention, through hydrogel fixation and synergistic effect of lysozyme and Au-TiO2, splitting decomposition of pathogenic bacteria on an interface layer is promoted, in-situ nucleic acid amplification is realized, and the method has an extremely high application value for on-site rapid detection of pathogenic bacteria of agricultural products.

Holding type listeria monocytogenes specific molecular target and rapid detection method thereof

Resumen de: CN122060880A

The invention discloses a persistent listeria monocytogenes specific molecular target and a rapid detection method thereof, and belongs to the technical field of microbiological detection. The molecular target is obtained through bioinformatics analysis and screening, is further screened through specific experimental verification, and is respectively specific genome sequence fragments of six retention types of listeria monocytogenes CC5, CC8, CC31, CC87, CC121 and CC204. A corresponding specific primer group is designed based on the target, sample DNA is amplified through PCR, a product is subjected to visual analysis in combination with gel electrophoresis, and whether the target retention type strain exists or not can be judged according to the size of a band. The detection method has the advantages of being short in detection time, low in cost, simple and convenient to operate and high in specificity, results are accurate, judgment is easy, practicability is high, the limitation that traditional culture and identification periods are long, and phenotypic analysis steps are tedious is broken through, and the detection method is suitable for rapid screening of the persistent listeria monocytogenes in samples of food, environment and the like.

Kit and detection method for detecting multiple piglet diarrhea pathogens

Resumen de: CN122060931A

The invention provides a kit for detecting multiple piglet diarrhea pathogens and a detection method, and belongs to the technical field of biological information detection. The kit comprises a reaction reagent, wherein the reaction reagent comprises a porcine epidemic diarrhea virus specific primer and a probe, a porcine delta coronavirus specific primer and a probe, a piglet pathogenic escherichia coli specific primer and a probe, a clostridium perfringens specific primer and a probe, a buffer solution, an enzyme mixed solution and a freeze-drying protective additive. The detection method adopts the kit for detecting various piglet diarrhea pathogens. According to the kit disclosed by the invention, simultaneous detection of four target genes including the porcine epidemic diarrhea virus, the porcine delta coronavirus, the piglet pathogenic escherichia coli and the clostridium perfringens can be realized only through one-time amplification, so that the detection time is greatly shortened while the detection cost is reduced; the kit has extremely high specificity, sensitivity and repeatability, and the primers and the probes do not interfere with each other; good normal-temperature storage stability is also realized.

Primer group, kit and detection method for detecting salmonella enteritidis

Resumen de: CN122060881A

The invention belongs to the technical field of microbiological detection and molecular diagnosis, and discloses a primer group, a kit and a detection method for detecting salmonella enteritidis, the kit comprises an immunocapture component, an isothermal amplification component, a CRISPR/Cas12a detection component and auxiliary components; the isothermal amplification component is any one of an RPA (recombinase polymerase amplification) component or an LAMP (loop-mediated isothermal amplification) component, the RPA component comprises a specific primer group targeting 86th to 212th nucleotides in a salmonella enteritidis hilA gene coding region, and the LAMP component comprises a specific primer group targeting 58th to 250th nucleotides in the salmonella enteritidis hilA gene coding region. The primer group, the kit and the detection method for detecting the salmonella enteritidis successfully integrate three key technologies of RAP and LAMP rapid isothermal amplification, CRISPR/Cas12a high-specificity recognition and trans-cleavage and immunomagnetic bead targeted enrichment, and creatively provide the primer group, the kit and the detection method for detecting the salmonella enteritidis.

Rapid and accurate detection method for pathogenic bacteria

Resumen de: CN122060834A

The invention discloses a rapid and accurate detection method for pathogenic bacteria. The method comprises the following steps: immobilizing bacteriophages on the surfaces of magnetic nanoparticles through physical adsorption or chemical reaction, selectively enriching pathogenic bacteria in a complex sample by adopting the bacteriophage functionalized magnetic nanoparticles, quickly cracking the pathogenic bacteria on the surfaces of the magnetic nanoparticles by using CTAB (cetyltrimethyl ammonium bromide), releasing ATP in the bacteria, and quantitatively analyzing ATP by using bioluminescence. Further calculating the content of the pathogenic bacteria; the detection method is free of enrichment and amplification, can complete capture, enrichment and detection of pathogenic bacteria within 20 minutes, and can specifically detect viable bacteria of target bacteria in various complex matrix (such as food) samples due to the fact that bacteriophages are adopted as recognition molecules.

Respiratory pathogen convection amplification primer pair, probe and multiple detection application

Resumen de: CN122038620A

The invention belongs to the field of biomedical treatment, and particularly relates to a convective amplification primer pair and probe for respiratory tract pathogenic bacteria and multiple detection application of the convective amplification primer pair and probe. The convection amplification primer pair comprises a legionella pneumophila primer pair, a mycoplasma pneumoniae primer pair and a bordetella pertussis primer pair, and the legionella pneumophila primer pair, the mycoplasma pneumoniae primer pair and the bordetella pertussis primer pair are respectively composed of an upstream primer and a downstream primer; each of the upstream primer and the downstream primer comprises a target nucleic acid complementary sequence and an interface sequence, and the interface sequence is located at the 5'end of the target nucleic acid complementary sequence and is not complementary with target nucleic acid; the target nucleic acid complementary sequence has a folded sequence, and the folded sequence is complementary with the interface sequence, so that the primer sequence has a curved hairpin structure, a probe is designed, and a respiratory tract pathogenic bacteria multiple detection kit is prepared. The multiple detection can avoid non-specific amplification and improve the detection sensitivity.

RPA-CRISPR method for rapidly detecting pathogens in air

Resumen de: CN122038544A

The invention belongs to the technical field of environmental biological safety monitoring, and particularly relates to an RPA-CRISPR method for rapidly detecting pathogens in air. According to the invention, a two-step detection strategy integrating RPA isothermal amplification and CRISPR detection is established; the method specifically comprises the following steps: firstly, acquiring genome DNA of an air sample, and carrying out specific amplification on a trace target by utilizing an RPA technology; then, a product is introduced into a CRISPR-Cas12a system, and the trans-shear activity is recognized and activated by utilizing the specificity of crRNA, so that signal cascade amplification is realized. Pseudomonas aeruginosa is used as a model pathogen, and a primer and probe combination is designed aiming at a specific gene of the pseudomonas aeruginosa. Results show that the method can complete detection within 10-15 minutes at the soonest, the lowest detection limit can reach a single copy level (100 copies/L), and the method has the advantages of high environmental interference resistance, high sensitivity and no need of large instruments, and can meet the on-site rapid screening requirements of air pathogen aerosol in public places, medical environments and other scenes.

Method for detecting multiple pathogenic bacteria by using magnetic separation time difference

Resumen de: CN122042964A

The invention relates to a method for detecting multiple pathogenic bacteria by using magnetic separation time difference, and belongs to the technical field of microbiological analysis and detection. According to the invention, the dual-mode aptamer sensor is integrated, so that rapid and accurate multiple pathogenic bacteria detection can be realized. The core of the time separation system based on magnetic coding comprises fluorescent magnetic coding nanoparticles, a gold nanoparticle-aptamer and a fluorescent-ultraviolet dual-mode probe. A preparation process of the fluorescent-ultraviolet dual-mode probe specifically comprises the following steps: sequentially anchoring quantum dots and specific cDNA on the surface of a magnetic coding nanosphere; and carrying out functional combination with aptamer-modified gold nanoparticles to finally synthesize the dual-mode probe with both fluorescence signal response and ultraviolet absorption response. The probe can realize targeted recognition and separation detection of various pathogenic bacteria through specific binding of the surface aptamer and target pathogenic bacteria.

High-flux automatic detection system for food pathogenic bacteria of micro-fluidic chip

Resumen de: CN122042956A

The invention relates to the technical field of food detection, and discloses a high-flux automatic detection system for food pathogenic bacteria of a micro-fluidic chip, which solves the problem that the micro-fluidic chip is inconvenient to quickly clean at present, and comprises a box body and the micro-fluidic chip, the outer side of the box body close to the bottom is fixedly connected with a liquid discharge pipe, and the liquid discharge pipe is fixedly connected with the micro-fluidic chip. A cleaning mechanism is arranged on the box body, a clamping mechanism is arranged in the box body, a mounting mechanism is arranged at the bottom of the micro-fluidic chip, a plurality of reaction cavities are formed in the micro-fluidic chip at equal intervals, a channel is formed in the end part of each reaction cavity, and a plurality of hoses are fixedly connected to the outer side of the micro-fluidic chip at equal intervals; one end, far away from the micro-fluidic chip, of each hose is fixedly connected with a hard tube, and each hose is communicated with each reaction cavity; according to the invention, waste liquid in the reaction cavity and the channel can be rapidly discharged, so that the micro-fluidic chip can be fully and rapidly cleaned without being disassembled.

Convection amplification primer group, probe and kit for multiple detection of lower respiratory pathogens

Resumen de: CN122038621A

The invention belongs to the field of biomedical treatment, and particularly relates to a convection amplification primer group, a probe and a kit for multiple detection of lower respiratory pathogens. The convection amplification primer pair comprises a legionella pneumophila primer pair, an adenovirus primer pair and a chlamydia psittaci primer pair, primer sequences in the legionella pneumophila primer pair, the adenovirus primer pair and the chlamydia psittaci primer pair respectively comprise a target nucleic acid complementary sequence and an interface sequence, and the interface sequence is located at the 5'end of the target nucleic acid sequence; the nucleic acid is not complementary with the target nucleic acid; the target nucleic acid complementary sequence has a folded sequence, and the folded sequence is complementary to the interface sequence, so that the primer sequence has a bent hairpin structure. Meanwhile, a primer probe is designed, and a respiratory tract pathogenic bacteria multiple detection kit is prepared. The multiple detection can avoid non-specific amplification and improve the detection sensitivity.

Primer probe group for simultaneously detecting six pathogenic bacteria, multiple fluorescent quantitative PCR (Polymerase Chain Reaction) method and kit

Resumen de: CN122038605A

The invention relates to a primer probe group, a multiplex fluorescent quantitative PCR method and a kit for simultaneously detecting six pathogenic bacteria, and belongs to the technical field of molecular biology, the primer probe group comprises primer sequences and probe sequences shown in SEQ ID No.1-18 in a table 1; the 5'end of each probe sequence is modified with a reporter group, and the 3 'end of each probe sequence is modified with a quenching group; the reporter group is Cy5, FAM and VIC, and the quenching group is Eclipse. The invention provides a method for simultaneously detecting six pathogenic bacteria including vibrio parahaemolyticus, salmonella, staphylococcus aureus, escherichia coli O157: H7, listeria monocytogenes and shigella in the same reaction system by combining a multiple real-time fluorescent quantitative PCR (Polymerase Chain Reaction) technology. The multiplex real-time fluorescent quantitative PCR kit provided by the invention is used for detecting the six pathogenic bacteria, can improve the detection sensitivity and specificity, greatly improves the detection efficiency, and overcomes the defects of complex steps, long detection period and incapability of adapting to large-scale rapid detection in the conventional detection method.

Biosensor detection reagent for detecting salmonella typhimurium and preparation method

Resumen de: CN122038409A

The invention discloses a biosensor detection reagent for detecting salmonella typhimurium and a preparation method, and belongs to the technical field of biomedicine. According to the invention, a DNA sequence of an amino-modified nucleic acid aptamer for detecting salmonella typhimurium is screened out and is shown as SEQ ID No: 1. Based on the nucleic acid aptamer, amino modified aptamer-fluorescent nano-enzyme serving as an energy donor and sulfydryl modified aptamer-gold nanoparticles serving as an energy receptor are mixed according to the volume ratio of 1: 1, and the biosensor detection reagent is prepared. The linear detection range of the biosensor detection reagent on salmonella typhimurium is 101-107 CFU/mL, the linear correlation coefficient R2 is equal to 0.9981, and the detection limit is as low as 1.527 CFU/mL. The biosensor detection reagent disclosed by the invention still keeps good selectivity and stability in a complex environment with high protein, high fat and high salt, and is suitable for rapid field detection in the fields of food safety and medical diagnosis.

Multiple qPCR (quantitative polymerase chain reaction) primer group and kit for detecting core pathogenic bacteria in aquaculture and application of multiple qPCR primer group and kit

Resumen de: CN122038618A

The invention discloses multiple qPCR (quantitative polymerase chain reaction) primers and a kit for detecting core pathogenic bacteria in aquaculture and application of the multiple qPCR primers and the kit. The multiple qPCR primer group comprises a primer combination A (SEQ ID NO.1-3) for detecting edwardsiella tarda, a primer combination B (SEQ ID NO.4-6) for detecting streptococcus iniae and a primer combination C (SEQ ID NO.7-9) for detecting aeromonas salmonicida. The invention also provides a kit containing the primer group and a detection method. A single-tube multiple detection system is constructed for three pathogenic bacteria which are most harmful in aquaculture, detection is rapid, and compared with a traditional culture method, the period is greatly shortened; the method is high in flux, low in cost and suitable for large-scale monitoring; and the kit has strong specificity and high sensitivity, and can realize early screening. The invention provides an effective tool for rapid, accurate and high-throughput detection of core pathogenic bacteria in aquaculture.

DETECTION OF AN AMPHIPHILE USING VISUAL INSPECTION OF A LIGAND-MODIFIED SUBSTRATE

Resumen de: US20260133191A1

Disclosed herein are compositions, devices, systems, and methods for detection of an amphiphile using visual inspection of a ligand-modified substrate. For example, disclosed herein are assays for detection of an amphiphile via visual inspection, the assay comprising a ligand-modified substrate and a film of lubricant disposed on the ligand-modified substrate. Also disclosed herein are methods of use of any of the assays disclosed herein. In some examples, the methods comprise contacting any of the assays disclosed herein with a liquid sample; tilting the assay; and visually inspecting the liquid sample disposed on the assay to determine a property of the liquid sample. In some examples, the amphiphile comprises a biological amphiphile, such as an endotoxin. In some examples, the biological amphiphile comprises an endotoxin secreted by a gram-negative bacteria, such as Escherichia coli.

METHODS AND MEANS FOR DETECTION OF LEGIONELLA

Resumen de: EP4488391A1

The invention relates to methods for determining whether a sample comprises Legionella pneumophila, the method comprising performing an isothermal nucleic acid amplification (iNAAT) reaction, preferably loop-mediated isothermal amplification (LAMP), with said sample with a primer set specific for the Legionella pneumophila DotB or MIP gene and determining whether the sample comprises an amplification product of the amplification reaction. The invention further relates to primer sets specific for the Legionella pneumophila DotB or MIP gene that are useful in such method, and kits of part comprising such primer set.

A SYSTEM FOR RAPIDLY DETECTING AND IDENTIFYING MICROORGANISMS ON BIOLOGICAL SURFACES (E.G. FISH SKIN SURFACES) AND/OR NON-BIOLOGICAL SURFACES (E.G. HABITAT OR IC SURFACES) WITH A FLEXIBLE DISSOLVABLE CLOTH AND CORRESPONDING METHODS

Nº publicación: EP4741805A1 13/05/2026

Solicitante:

CV TROJKA TEGEN PANDEMIEEN [NL]
CV Trojka tegen Pandemie\u00EBn

Resumen de: EP4741805A1

0001 The current invention comprises systems and methods for the detection of food related bacteria, such as listeria, e. coli and s. aureus in a mobile and modular design. The combination of a dissolvable flexible cloth as sampling means, a multiple target sensor, smart readout optics and spectral processing enables to instantly ascertain, verify and validate the presence of specific microbes. The main application is the monitoring of the food and food production in order to prevent the coming to the market of bacteria invested products, such as fish, chicken or other food products destined for human consumption.