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CHLAMYDOMONAS REINHARDTII CHLOROPLAST-SACCHAROMYCES CEREVISIAE-ESCHERICHIA COLI SHUTTLE VECTOR AND CONSTRUCTION METHOD AND USE THEREOF

Resumen de: US2025084424A1

A Chlamydomonas reinhardtii chloroplast-Saccharomyces cerevisiae-Escherichia coli shuttle vector and a construction method and use thereof. The construction method includes design, chemical synthesis, assembly and identification of a shuttle vector, as well as replication of the shuttle vector in Escherichia coli. The constructed shuttle vector is capable of shuttling and replicating among the Escherichia coli, a yeast, and a Chlamydomonas reinhardtii chloroplast. The shuttle vector of the present invention includes a replication element (CEN) for the yeast, a replication element (ori) for the Escherichia coli, and a Chlamydomonas reinhardtii chloroplast genome (SynCp V1.0). According to the present invention, a totally chemically synthesized shuttle vector is transformed into Chlamydomonas reinhardtii cells, biological functions of the shuttle vector are achieved, a chloroplast genome is extracted and transferred into Escherichia coli, successful replication of the shuttle vector in the Escherichia coli is achieved.

FERMENTATION METHODS USING ACETOGENIC CARBOXYDOTROPHIC BACTERIA

Resumen de: US2025084444A1

Disclosed are methods for producing from a gaseous substrate comprising CO, CO2, and optionally H2 at least one oxygenated product. Also provided are methods of using a broth deficient in thiamine or thiazole-containing thiamine precursors as a screening tool for suitable ethanol producing acetogenic carboxydotrophic bacteria strains, operating a syngas fermentation without the addition of thiamine or thiazole-containing thiamine precursors, controlling bacterial contamination in a bioreactor, and methods for preparing animal feed and fertilizer.

ORGANISMS AND METHODS OF USE THEREOF

Resumen de: US2025084445A1

Disclosed are organisms comprising a highly expressed form of EC 1.2.3.1, and methods of their use including methods for efficient fermentation broth recycle, methods for improving bottoms recycle, methods for converting CO, CO2, and optionally H2 to ethanol and other oxygenated products, methods for preparing animal feed, and methods for preparing fertilizer.

ENERGY STORAGE METHOD AND DEVICE FOR BIOMASS CASCADE PYROLYSIS COUPLED WITH NEW ENERGY POWER GENERATION

Resumen de: US2025084805A1

The provided is energy storage method and device for biomass cascade pyrolysis coupled with new energy power generation. The key point of the technical solution is that, with inexpensive, clean and safe biomass as energy storage medium, the redundant unstable electric energy is converted by a cascade pyrolysis energy storage system into an easy-to-store liquid and solid chemical energy in biomass pyrolytic products, and based on use requirements, can be further converted into clean fuels for power generation or exported renewable chemicals, so as to realize continuous stable output of the new energy power generation systems. Furthermore, the cascade pyrolysis energy storage system can, based on the principle of “energy level matching”, fully recover and utilize the electric energy, high-temperature heat energy and low-temperature heat energy generated in pyrolysis processes, thereby maximizing the energy utilization efficiency of the system.

PROVIDING AN OIL COMPOSITION THROUGH FERMENTATION OF BIOMASS WITH A YEAST

Resumen de: AU2023338542A1

The invention provides a method for producing an oil composition (100) from biomass (200), wherein the method comprises: a preparation stage (11) comprising providing a growth medium (300), wherein the growth medium (300) comprises the biomass (200), wherein the growth medium (300) comprises 1 - 10 wt% dry matter, 0.02 - 30 wt% of a carbon source, 0.01 - 5 wt% of a nitrogen source, 0.00001 - 0.05 wt. % of a magnesium source, and wherein the growth medium (300) has a pH selected from the range of 3.0 - 8.0, wherein the biomass (200) comprises a non-pasteurized biomass (220); a fermentation stage (12) comprising (a) inoculating the growth medium (300) with a yeast cell (400), thereby providing an cell suspension (500), and (b) controlling a temperature of the cell suspension (500) in the range of 10-40 ºC, wherein the yeast cell (400) is selected from the species

FORMATE DEHYDROGENASE VARIANTS AND METHODS OF USE

Resumen de: US2025084387A1

The disclosure provides polypeptides and encoding nucleic acids of engineered formate dehydrogenases. The disclosure also provides cells expressing an engineered formate dehydrogenase. The disclosure further provides methods for producing a bioderived compound comprising culturing cells expressing an engineered formate dehydrogenase. Where the engineered formate dehydrogenase is capable of catalyzing a conversion.

METHOD AND SYSTEM FOR PRODUCING A FUEL FROM BIOGAS

Resumen de: US2025084334A1

A method for providing a fuel includes providing biogas from a plurality of biogas sources, the biogas from each biogas source produced in a process comprising filling a vessel with raw biogas or partially purified biogas to a pressure of at least 1500 psig and transporting the filled vessel to a centralized processing facility by vehicle. A fuel is produced in a fuel production process that includes feeding the biogas transported to the centralized processing facility to a biogas upgrading system that is configured to provide a carbon dioxide removed from the biogas. The removed carbon dioxide is provided for transport by vehicle and/or pipeline and/or sequestered to offset greenhouse gas emissions attributed to compressing the biogas for transport.

MICROWAVE BIOMASS TORREFACTION

Resumen de: EP4520806A1

The invention is in the field of biomass processing. In particular, the present invention is directed to a method and system for torrefaction of biomass, and to bio-coal and/or bio-char obtainable by such torrefaction.There is provided a process for the production of bio-coal and/or bio-char, comprising a torrefaction step in which biomass is irradiated in a heating chamber using microwave radiation, wherein biomass is continuously fed to the heating chamber and torrefied biomass is continuously removed from the heating chamber.

CONTROL OF PYROLYSIS CONDITIONS FOR THE PRODUCTION OF DESIRABLE COMPOUNDS

Resumen de: US2025072428A1

The present invention relates to (1) a method for producing biochar having bio stimulating effects, (2) biochar that has been produced to having increased bio stimulating effects, (3) a method for capturing material extracted from biochar and (4) biochar extracts, comprising desired compounds extracted from biochar and suspended in an extract solution. The methods above all involve obtaining a particular biomass source and pyrolyzing the biomass source under specific conditions to increase the bio stimulating effects of the biochar. The method for capturing the material extracted from the biochar produced to have such stimulating effects includes contacting the biochar with a treating liquid, separating the biochar from the biochar extract, collecting the biochar extract from which the biochar has been separated and resuspending the biochar extract in an modified aqueous solution for further use and/or processing separate from, or prior to, disposal. The biochar extract suspended in solution then includes desired compounds (a) at least 0.1 ppm of one or more of the following phenylpropanoid, phenylalanine, flavonoids, sterols, terpenes, terpenoids, tannins, coumarins, benzaldehydes and phenols; (b) at least 0.1 g/L of humics, fulvics or a combination of both humics and fulvics and/or (c) one or more of the following salicylic acid, jasmonic acid, abscisic acid, cytokinin, gibberellic acid and ethylene, by way of example.

EFFICIENT HEAT-INDUCTION DECOKING DEVICE AND METHOD FOR THERMAL CONVERSION OF SOLID FUELS

Resumen de: US2025075130A1

The provided is a novel heat-induction decoking device and method tailored for the thermal conversion of solid fuels. The efficient heat-induction decoking device for the thermal conversion of the solid fuels includes: a decoking tank body, which is configured to condense a macromolecular tar in a pyrolysis gas and discharge the condensed tar from a bottom to a tar tank for storage; an electromagnetic induction heating system, which is disposed on an outer wall surface of the decoking tank body to heat the outer wall surface of the decoking tank body; an ultrasonic vibration decoking system, which is disposed on the decoking tank body to enable the decoking tank body to generate vibration by ultrasonic wave so as to enhance tar fluidity. This innovative design not only reduces energy consumption and equipment wear but also extends system operational lifespan, thereby offering substantial improvements in economic and environmental sustainability.

PROCESS FOR PRODUCING HIGH-CARBON BIOGENIC REAGENTS

Resumen de: US2025075141A1

This invention provides processes and systems for converting biomass into high carbon biogenic reagents that are suitable for a variety of commercial applications. Some embodiments employ pyrolysis in the presence of an inert gas to generate hot pyrolyzed solids, condensable vapors, and non-condensable gases, followed by separation of vapors and gases, and cooling of the hot pyrolyzed solids in the presence of the inert gas. Additives may be introduced during processing or combined with the reagent, or both. The biogenic reagent may include at least 70 wt %, 80 wt %, 90 wt %, 95 wt %, or more total carbon on a dry basis. The biogenic reagent may have an energy content of at least 12,000 Btu/lb, 13,000 Btu/lb, 14,000 Btu/lb, or 14,500 Btu/lb on a dry basis. The biogenic reagent may be formed into fine powders, or structural objects. The structural objects may have a structure and/or strength that derive from the feedstock, heat rate, and additives.

PROCESS FOR THE PRODUCTION OF A TECHNICAL ENZYME COMPOSITION WITH LOW VISCOSITY PRODUCED BY A FILAMENTOUS FUNGUS

Resumen de: US2025075242A1

The present invention relates to a process for the production of a technical enzyme composition with low viscosity, a genetically modified filamentous fungus cell suitable for production of the technical enzyme composition, the use of such a genetically modified filamentous fungus cell for the production of the technical enzyme composition with low viscosity and a technical enzyme composition with low viscosity produced by such a process.

PRODUCTION OF ETHANOL WITH ONE OR MORE CO-PRODUCTS IN YEAST

Resumen de: US2025075235A1

The disclosure provides processes for the production of ethanol and one or more co-products from a fermentable carbon source. The ethanol and one or more co-products are produced in an ethanol-producing yeast modified to further produce the one or more co-products. The processes involve contacting a fermentable carbon source with the modified yeast in a fermentation medium, fermenting the yeast in the fermentation medium such that the yeast produces ethanol and the one or more co-products from the fermentable carbon source, and isolating the ethanol and the one or more co-products. The modified yeast is an ethanol-producing yeast that produces ethanol in a greater concentration than the one or more co-products. Additionally, the disclosure provides the modified yeast disclosed herein.

XYLANASES WITH ENHANCED THERMOTOLERANCE AND USES THEREOF

Resumen de: WO2023212679A2

A thermotolerant xylanase enzyme that is a variant of SEQ ID NO:2, wherein the variant comprises at least one amino acid substitution at a position selected from the group consisting of 90, 105, 114, and 115 and/or comprises any of the following sets of substitutions: (a) G48C and T206C; (b) Y123V, S125C and N171C; (c) S114C, Y123V, S125C and N171C; (d) Y123V, S125C, N171C, G48C and T206C; (e) S114C, Y123V, S125C, N171C, G48C and T206C; wherein the substitution or substitutions increases the thermotolerance of the xylanase relative to a parent xylanase lacking the substitution(s).

PRODUCTION OF FATTY ACID ALKYL ESTERS

Resumen de: CN118765328A

The present invention provides methods for producing fatty acid alkyl esters. The method comprises providing a substrate comprising a triglyceride, a diglyceride, a monoglyceride, a free fatty acid, or any combination thereof, and reacting the substrate with an enzyme composition comprising a sn-1, a lipase at position 3, and a lipase at position sn-2 to produce a fatty acid alkyl ester.

Technology of using natural materials for solar drying of sewage sludge and production of briquettes based on sewage sludge

Resumen de: PL445919A1

Technologia wykorzystania naturalnych materiałów do suszenia solarnego osadów ściekowych i produkcji brykietów na bazie osadu ściekowego według wynalazku polega na tym, że odwodniony osad nadmierny, o maksymalnym uwodnieniu (w zakresie 85% — 90%) lub mniejszym, pochodzący z oczyszczania ścieków miesza się w stosunku objętościowym 45% z odpadowym materiałem ligno-celulozowym w postaci słomy, a następnie suszy solarnie i brykietuje się, z wydajnością do 160 kg/h tak, że średnica brykietu wynosi 8 cm, a brykiet ma otwór wzdłużny, temperatura procesu brykietowania regulowana jest od 150°C do 230°C, prasa jest prasą śrubową.

CONTAMINATION CONTROL WHEN GROWING YEASTS

Resumen de: US2025066714A1

A method for contamination control when growing yeasts is provided. Bacterial contamination is controlled by using urea as the primary nitrogen source while simultaneously limiting the amount of nickel available to contaminating bacteria. Bacteria require nickel as a cofactor for urease enzymes in order to use urea for growth while yeasts do not require nickel as a cofactor for any enzymes. Nickel is limited by using titanium in plate heat exchangers instead of stainless steel. Ethyl carbamate is limited by using a carbon/nitrogen ratio that consumes all urea during fermentation and by separating co-products after fermentation and before distillation. Yeast recycling is performed by using either single-step or two-step centrifugation, without acid washing. This method enables yeast recycling with sugarcane ethanol and sugar beet ethanol production. This method also enables yeast recycling with corn ethanol and grain ethanol production with coproduct recovery after fermentation and before distillation.

METHOD OF MANUFACTURING DENSIFIED, TORREFIED BIOMASS PARTICULATES

Resumen de: WO2025040761A1

A method of manufacturing densified, torrefied biomass particulates is disclosed. The method comprises at least : a torrefaction step of torrefying a particulate biomass material under torrefaction conditions; a densification step of densifying the torrefied particulate biomass material from the torrefaction step, thereby obtaining densified torrefied biomass particulates; and a thermal post treatment step of heat treating the densified and torrefied biomass material particulates from the densification step under torrefaction conditions. The thermal post treatment step improves the durability of the densified, torrefied biomass particulates.

PYROLYSER

Resumen de: US2025066671A1

A pyrolyser including (i) a pyrolysis chamber defining an interior compartment having an upper portion and a lower portion, wherein the pyrolysis chamber includes a feed inlet, a product outlet, an exhaust gas outlet, and an operational length comprising a linear distance from the feed inlet to the product outlet; (ii) a trough located in the lower portion of the interior compartment and extending through the pyrolysis chamber along the operational length of the pyrolysis chamber; and (iii) a material handling system comprising an paddle auger located within the trough and configured to convey biomass from the feed inlet towards the product outlet; wherein the paddle auger comprises an auger shaft, a plurality of sets of paddles directly or indirectly connected to the auger shaft, and a plurality of flights interconnecting the plurality of paddles is provided.

Redox flow battery electrolytes

Resumen de: AU2025200854A1

REDOX FLOW BATTER ELECTROLYTES Abstract The present invention relates to novel combinations of redox active compounds for use as redox flow battery electrolytes. The invention further provides kits comprising these combinations, redox flow batteries, and method using the combinations, kits and redox flow batteries of the invention.

PYROLYSER

Resumen de: WO2025042442A1

A pyrolyser including (i) a pyrolysis chamber defining an interior compartment having an upper portion and a lower portion, wherein the pyrolysis chamber includes a feed inlet, a product outlet, an exhaust gas outlet, and an operational length comprising a linear distance from the feed inlet to the product outlet; (ii) a trough located in the lower portion of the interior compartment and extending through the pyrolysis chamber along the operational length of the pyrolysis chamber; and (iii) a material handling system comprising an paddle auger located within the trough and configured to convey biomass from the feed inlet towards the product outlet; wherein the paddle auger comprises an auger shaft, a plurality of sets of paddles directly or indirectly connected to the auger shaft, and a plurality of flights interconnecting the plurality of paddles is provided.

CONVERSION OF GLYCEROL TO FUEL-RANGE ORGANIC COMPOUNDS

Resumen de: WO2023203465A1

A process for producing fuel-range organic oxygen- containing compounds is provided. The process includes converting glycerol in the presence of a metal oxide catalyst. The fuel-range organic oxygen-containing compounds can be deoxygenated to produce gasoline and jet fuels or fuel blending components.

METHOD FOR PRODUCING 5-HALOMETHYLFURFURAL AND SYSTEM THEREFOR

Resumen de: US2025059148A1

According to the present disclosure, by forming normal pressure conditions in a reaction system through application of a reflux system to an aqueous phase in the presence of sulfuric acid and an inorganic salt in the dehydration reaction of biomass-derived sugar components in an open system, there is no need to include a high-pressure vessel required in a conventional 5-halomethylfurfural production process, there is no risk of safety accidents, including the explosion of a high-pressure vessel and the leakage of strong acid gas, and there is a remarkable effect in that yield is improved three-fold or more compared to the case of using a conventionally used acid catalyst under the normal pressure conditions.

CARBON CAPTURE IN FERMENTATION FOR COMMODITY CHEMICALS

Resumen de: US2025059146A1

The disclosure relates to methods of capturing carbon by microbial fermentation of a gaseous substrate comprising CO into one or more first products which, in turn, may be incorporated into an article of manufacture or one or more second products. Further, the disclosure relates to improving carbon capture and/or efficiency.

PROCESS OF EXTRACTING OIL FROM THIN STILLAGE

Nº publicación: US2025059523A1 20/02/2025

Solicitante:

NOVOZYMES AS [DK]
Novozymes A/S

Resumen de: US2025059523A1

A process of recovering oil, comprising (a) converting a starch-containing material into dextrins with an alpha-amylase; (b) saccharifying the dextrins using a carbohydrate source generating enzyme to form a sugar; (c) fermenting the sugar in a fermentation medium into a fermentation product using a fermenting organism; (d) recovering the fermentation product to form a whole stillage; (e) separating the whole stillage into thin stillage and wet cake; (e′) optionally concentrating the thin stillage into syrup; (f) recovering oil from the thin stillage and/or optionally the syrup, wherein a protease and a phospholipase are present and/or added during steps (a) to (c). Use of a protease and a phospholipase for increasing oil recovery yields from thin stillage and/or syrup in a fermentation product production process.