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Recombinant attenuated salmonella for expressing pseudostellaria canis metalloproteinase TcMMP-13 and application of recombinant attenuated salmonella

Resumen de: CN121265761A

The invention discloses recombinant attenuated salmonella for expressing metalloproteinase TcMMP-13 of ascaris canis and application of the recombinant attenuated salmonella. The recombinant attenuated salmonella is characterized in that the attenuated salmonella expresses the metalloproteinase TcMMP-13. The vaccine has no obvious influence on mouse body health, the insect reduction rate is far higher than the insect reduction rate threshold values of 40% and 50% provided by the World Health Organization (WHO) in schistosome and hookworm vaccine research and development guidelines, and the recombinant attenuated salmonella vector oral vaccine has excellent protection effect and potential application value.

C8 esterase activated double-lock chemiluminescent probe, preparation method thereof and application of C8 esterase activated double-lock chemiluminescent probe in preparation of detection kit for detecting salmonella

Resumen de: CN121270430A

The invention relates to the technical field of chemical analysis and detection, and discloses a C8 esterase activated double-lock chemiluminescence probe, a preparation method thereof and application of the C8 esterase activated double-lock chemiluminescence probe in preparation of a salmonella detection kit, the double-lock chemiluminescence probe is DSCP-CBCN, and the structural formula of the double-lock chemiluminescence probe is shown in the specification. The DSCP-CBCN probe is composed of a C8 esterase cleavable substrate, a phenoxy cyclobutane chemiluminophor and a self-eliminating connexon p-hydroxy benzyl alcohol. After the DSCP-CBCN probe and salmonella are incubated, sodium hypochlorite and hydrogen peroxide are added to generate remarkable chemiluminescence, POCT rapid detection of salmonella can be realized by combining a chemiluminescence microplate reader, the bacterial culture time is shortened to 3-7 hours, the limit of detection (LOD) value is as low as 1.9 CFU/mL, and the sensitivity is improved by 1008 times compared with that of a commercial MUCAP probe. The method is suitable for salmonella large-scale screening in the fields of clinical pathogen diagnosis, food safety detection and environmental health monitoring.

一种改良型鞭毛蛋白及其应用

Resumen de: CN121248736A

本发明属于生物技术领域，具体涉及一种能够增强人源TLR5结合亲和力的鼠伤寒沙门菌鞭毛蛋白及其应用。本发明通过筛选并确定鞭毛蛋白的关键氨基酸突变位点，采用定点突变技术在大肠杆菌系统中实现重组表达。经体外实验验证，该衍生物能够显著激活TLR5配体功能。本发明解决了现有鞭毛蛋白在激活人源TLR5活性方面效果有限的问题，为鞭毛蛋白在疫苗、免疫治疗剂以及放射防护剂领域的应用提供了新的技术方案和理论基础。

PCR kit for triple PCR detection of escherichia coli, salmonella enteritidis and riemerella anatipestifer and application of PCR kit

Resumen de: CN121249921A

The invention belongs to the technical field of triple PCR detection PCR kits, and particularly relates to a triple PCR detection PCR kit for escherichia coli, salmonella enteritidis and riemerella anatipestifer and application of the triple PCR detection PCR kit. According to the kit, specific primers are designed by taking an escherichia coli alkaline phosphatase gene, a salmonella enteritidis invasion protein A gene and a riemerella anatipestifer helicase gene as target genes, the specific primers comprise a primer pair aiming at the phoA gene, the sequence of an upstream primer phoA-F is 5 '-TACAGGTACTGCGGGCTTATC-3', the sequence of a downstream primer phoA-R is 5 '-CTTACCGGGAATACATCACA-3', and the length of an amplified target fragment is 622bp; according to the primer pair for the invA gene, the sequence of an upstream primer invA-F is 5 '-AAAAGAAGGGTCGTCGTTAG-3', the sequence of a downstream primer invA-R is 5 '-GGAAGGTACTGCCAGAGGTC-3', the length of an amplified target fragment is 801 bp, and according to the primer pair for the dnaB gene, the sequence of an upstream primer dnaB-F is 5 '-GACGCTATATCATAGATTTAAC-3', the sequence of a downstream primer dnaB-R is 5 '-TCCACCGCTATATATTCTAG-3', and the length of the amplified target fragment is 431 bp.

Preparation of hesperidin carbon dots based on PVP modification and application of hesperidin carbon dots to pork preservation

Resumen de: CN121242078A

The invention discloses a preparation method of hesperidin carbon dots based on PVP modification and pork fresh-keeping application, and aims to solve the problems that hesperidin is poor in water solubility and a traditional nano fresh-keeping material is high in toxicity. The preparation method comprises the following steps: dissolving hesperidin and PVP (Polyvinyl Pyrrolidone) in a mass ratio of (1: 0.5)-(1: 2) in 50-70mL of absolute ethyl alcohol, and stirring for 20-40 minutes; and transferring into a high-pressure kettle, carrying out solvothermal reaction at 170-190 DEG C for 8-15 minutes, cooling, centrifuging at 8000-12000 revolutions per minute, filtering at 0.22 mu m, dialyzing the filtrate for 20-30 hours by using a 500Da dialysis bag, carrying out rotary concentration at 40-50 DEG C, and freeze-drying to obtain hesperidin carbon dot powder. The product is spherical, has a particle size of 3.0-4.5 nm, contains hydroxyl, amino and other functional groups, has an ultraviolet absorption peak at 241 nm, and has a maximum emission wavelength of 450 nm under excitation of 507 nm. When pork is preserved, pork slices are soaked in a 1-5 mg/mL carbon dot aqueous solution for 20-40 minutes and stored at 4 DEG C, the effect is optimal when the carbon dot aqueous solution is 5 mg/mL, microorganisms such as salmonella can be inhibited, lipid oxidation is delayed, and the quality guarantee period of the pork is prolonged by 2-3 days. Raw materials are green and cheap, preparati

一种增强TLR5配体活性的鞭毛蛋白突变体及其应用

Resumen de: CN121248735A

本发明属于生物技术领域，具体涉及一种具有增强TLR5配体活性的鞭毛蛋白突变体及其应用。选择鼠伤寒沙门氏菌鞭毛蛋白一个氨基酸突变位点，通过定点突变技术，利用大肠杆菌系统表达重组蛋白，经体外实验验证显示出良好的生物学活性，与野生型鞭毛蛋白突变体相比，能够更好的激活TLR5配体活性，为增强鞭毛蛋白佐剂和疫苗活性提供了良好的基础。

Pediococcus pentosaceus D17-sourced cytoplasmic membrane vesicle and application thereof

Resumen de: CN121249521A

The invention discloses a pediococcus pentosaceus D17-sourced cytoplasmic membrane vesicle and application thereof, and belongs to the technical field of microorganisms. The pediococcus pentosaceus D17 with excellent probiotic characteristics is separated from fresh excrement of Lichuan horses for the first time, the preservation number of the pediococcus pentosaceus D17 is CCTCC (China Center For Type Culture Collection) NO: M20252051, and meanwhile, the application of the pediococcus pentosaceus D17 and the cytoplasmic membrane vesicles of the pediococcus pentosaceus D17 in resisting salmonella infection is researched. Experimental verification shows that the separated and screened pediococcus pentosaceus D17 and the cytoplasmic membrane vesicles thereof have the effect of resisting salmonella infection and can promote expression of tight junction protein, and the treatment effect of the cytoplasmic membrane vesicles derived from the pediococcus pentosaceus D17 is superior to that of the pediococcus pentosaceus D17. Due to the fact that the cytoplasmic membrane vesicles are prepared through centrifugation of bacterial liquid supernatant, the cytoplasmic membrane vesicles are safer and free of drug resistance risks, and a new thought and a new approach are provided for application of the cytoplasmic membrane vesicles sourced from pediococcus pentosaceus to preparation of salmonella infection resisting products.

OMVOGENIC OUTER MEMBRANE PROTEIN ENGINEERING FOR OUTER MEMBRANE VESICLE PRODUCTION

Resumen de: WO2026006835A1

Disclosed are engineered outer membrane proteins (OMPs) that comprise one or more extracellular loops from the Salmonella enterica serovar Typhimurium protein PagC or a functional portion thereof. Methods of expressing the engineered outer membrane proteins in a cell, such as a Gram-negative bacteria, to increase the production of outer membrane vesicles are also provided.

Antibacterial peptide fragment intercepted from chitinase IDGF4 protein and application of antibacterial peptide fragment in preparation of antibacterial drugs

Resumen de: CN121249627A

The invention discloses an antibacterial peptide fragment intercepted from chitinase IDGF4 protein and application of the antibacterial peptide fragment in preparation of antibacterial drugs. According to the invention, short peptide fragments in chitinase IDGF4 protein are screened, three candidate peptides are obtained through chemical synthesis, the antibacterial performance of the candidate peptides is systematically evaluated, and the evaluation result shows that the peptide fragment with the amino acid sequence as shown in SEQ ID No.2 can effectively inhibit the growth of salmonella and staphylococcus aureus under the concentration of 1mg/mL; the antibacterial effect of the compound is confirmed in inhibition zone experiments, enzyme-labeled turbidimetry and growth curve monitoring, and the compound shows stable and remarkable antibacterial activity and can be used as a novel antibacterial molecule; the invention provides a new candidate sequence for developing the antibacterial peptide with high efficiency and low drug resistance risk, and has application potential in the aspect of preparing clinical antibacterial drugs.

Method for detecting mouse salmonella by using qPCR (quantitative polymerase chain reaction) technology instead of traditional sacrificial sentinel mouse

Resumen de: CN121227909A

The invention discloses a method for detecting mouse salmonella by using a qPCR (quantitative polymerase chain reaction) technology instead of a traditional sacrificial sentinel mouse, and a salmonella specific primer and probe combination comprises a forward primer with a sequence of 5 '-AGCGTACTGGAAAGGGAAAG-3', as shown in SEQ ID NO.1, a reverse primer with a sequence of 5 '-AGCGTACGAAAG-3', as shown in SEQ ID NO.2, a reverse primer with a sequence of 5 '-AGCGTACGAAGGAAAG-3', as shown in SEQ ID NO.3, the sequence of a reverse primer is 5-ATACCGCCAATAAAGTTCACAAAG-3 ', and the sequence of the reverse primer is as shown in SEQ ID NO. 2; the probe sequence of the probe is 5 '-FAM-CGTCACCTTGATAAACTCTATGCA-BHQ1-3', and the probe sequence of the probe is as shown in SEQ ID NO. 3. The method has the beneficial effects that a real-time fluorescent quantitative PCR method is established by utilizing the specific primer and the double-labeled fluorescent probe, so that rapid detection of salmonella pollution in facilities is realized; according to the method based on the environmental sample, use of additional animals is avoided, operation is easy and convenient, the result is accurate, and sensitivity and specificity are both superior to those of a traditional sentinel mouse method.

一种将猪霍乱沙门氏菌进行低内毒化改造的方法及其应用

Resumen de: CN121227763A

一种将猪霍乱沙门氏菌进行低内毒化改造的方法及其应用，它涉及疫苗领域，本发明通过基因工程技术对猪霍乱沙门氏菌弱毒株SC014的lipid A修饰，对猪霍乱沙门氏菌进行低内毒素化改造，成功构建了低内毒素的猪霍乱沙门氏菌突变体。改造后的猪霍乱沙门氏菌及其外膜囊泡在显著降低内毒素活性的同时，仍能有效激活免疫反应，实现了安全性与有效性的兼顾，为开发新型疫苗提供了新的思路和方法。

Application of peanut red skin procyanidine nano-liposome in inhibition of salmonella typhimurium

Resumen de: CN121220533A

The invention belongs to the technical field of food safety, and relates to application of peanut red skin proanthocyanidin nano-liposome in inhibition of salmonella typhimurium, and the peanut red skin proanthocyanidin nano-liposome causes cell death by causing permeability change of cell membranes and cell walls of salmonella typhimurium. According to the present invention, the inherent limitation of the natural antibacterial substance PSPc is overcome through the efficient delivery system of the nano-liposome, such that the salmonella typhimurium inhibition activity of the nano-liposome can be completely performed and durably maintained, the method is efficient and safe, the application potential in the food preservation aspect is provided, and the value-added utilization of the peanut resource is improved.

Detection method and application of mouse pathogenic bacteria

Resumen de: CN121227913A

The invention relates to a multiplex fluorescent quantitative PCR (Polymerase Chain Reaction) method for detecting mouse pathogenic bacteria, which can be used for simultaneously detecting four pathogens, namely salmonella, corynebacterium murine, pseudomonas aeruginosa and klebsiella pneumoniae.

Bacteria-enzyme synergistic product for bacteriostasis, odor removal and deodorization of pets as well as preparation method and application of bacterium-enzyme synergistic product

Resumen de: CN121221502A

The invention provides a bacterium-enzyme synergistic product for bacteriostasis, odor removal and deodorization of pets as well as a preparation method and application of the bacterium-enzyme synergistic product. The active ingredients of the product comprise a compound bacteria solution and a compound enzyme preparation, the composite bacterial liquid is prepared by compounding bacterial liquids of bacillus subtilis, bacillus amyloliquefaciens, lactobacillus acidophilus, rhodopseudomonas palustris and saccharomyces cerevisiae according to a specific volume ratio; the compound enzyme preparation is prepared by compounding protease, lipase, glucose oxidase and laccase according to a specific weight ratio. Through the synergistic effect of bacteria and enzymes, organic matters in pet excrement can be quickly decomposed, malodorous gases such as ammonia gas and hydrogen sulfide are eliminated from the source, and breeding of harmful bacteria such as staphylococcus aureus and salmonella is effectively inhibited. The product is safe, non-toxic, non-irritant and efficient and lasting in effect, can be used for spraying treatment on the body surface of a pet or the living environment of the pet, and solves the problems that a traditional physical or chemical deodorization method is short in effect and irritant.

Lactobacillus paracasei with effect of relieving inflammatory bowel disease and application of lactobacillus paracasei

Resumen de: CN121227591A

The invention discloses a lactobacillus paracasei strain with an effect of relieving inflammatory bowel diseases and application thereof, and belongs to the technical field of microbial fermentation. The strain is Lactobacillus paracasei JGSLP08, and the preservation number of the Lactobacillus paracasei JGSLP08 is CGMCC (China General Microbiological Culture Collection Center) NO.34274. The invention further discloses a preparation method of the Lactobacillus paracasei JGSLP08. The strain has the characteristics of strong acid resistance, bile salt resistance and gastrointestinal fluid resistance, can effectively inhibit salmonella typhimurium, escherichia coli and staphylococcus aureus, and has an excellent antioxidant effect. A DSS-induced colitis mouse model shows that the lactobacillus paracasei can effectively improve the DIA score of a mouse, regulate inflammatory factors, reduce oxidative stress injury, relieve inflammatory cell infiltration and protect the integrity of mucosal epithelium, so that the inflammatory bowel disease is relieved and/or treated.

Genetically engineered bacterium, medicine, fungicide, application of genetically engineered bacterium, medicine and fungicide and method for constructing genetically engineered bacterium

Resumen de: CN121203928A

The invention relates to the technical field of bioengineering, and discloses a genetically engineered bacterium, a medicine, a fungicide, application of the genetically engineered bacterium and a method for constructing the genetically engineered bacterium, the genetically engineered bacterium is attenuated salmonella typhimurium carrying plasmids, the plasmids are plasmids cloned with genes of GLP-1 analogues, and the GLP-1 analogues are 4 * GLP-1 and/or GLP-1-Fc; the amino acid sequence of the 4 * GLP-1 is as shown in SEQ ID NO. 1; the amino acid sequence of the GLP-1-Fc is as shown in SEQ ID NO. 2. The genetically engineered bacterium provided by the invention has the effects of reducing blood sugar, reducing blood fat, relieving fatty degeneration, reducing pancreatic tissue damage and/or liver tissue damage, reducing inflammatory cell infiltration, promoting insulin secretion of pancreatic beta cells, reducing lipid accumulation in liver tissues and the like, and has the effect of treating colon cancer. Therefore, the genetically engineered bacterium disclosed by the invention can be used as a medicine for preventing and/or treating the metabolic syndrome.

Novel broad-spectrum antibacterial peptide YHX-9 and application thereof

Resumen de: CN121202967A

The invention belongs to the technical field of biology, and relates to a novel broad-spectrum antibacterial peptide YHX-9 and application thereof. The amino acid sequence of the antibacterial peptide YHX-9 is as shown in SEQ ID No.1, and the antibacterial peptide YHX-9 is short in synthetic sequence, low in chemical synthesis difficulty and suitable for large-scale production; the antibacterial peptide YHX-9 has good antibacterial activity on staphylococcus aureus, listeria monocytogenes, streptococcus mutans, escherichia coli, salmonella typhimurium and pseudomonas aeruginosa, and has wide application prospects in the fields of food or medicines, cosmetics, agriculture, animal husbandry and the like.

Alpha spiral mutant of granulysin with improved antibacterial activity and application of alpha spiral mutant

Resumen de: CN121202988A

The invention relates to the technical field of agricultural biology, in particular to an alpha spiral mutant of granulysin with improved antibacterial activity and application of the alpha spiral mutant. The amino acid sequence of the alpha spiral mutant peptide of granulysin with improved antibacterial activity is as shown in SEQ ID No: 2. According to the present invention, the antibacterial activity of the granulysin mutant on different bacteria such as gram-negative bacteria salmonella typhimurium, escherichia coli and salmonella pullorum is enhanced;

Antibacterial peptide pig NK lysin mutant and application thereof

Resumen de: CN121202989A

The invention relates to the technical field of agricultural biology, in particular to an antibacterial peptide pig NK lysin mutant and application thereof. The amino acid of each alpha helix of the antibacterial peptide, especially the composition and position of basic amino acid and hydrophobic amino acid, is subjected to combined mutation, so that the antibacterial peptide with an enhanced antibacterial function is screened. The antibacterial activity of the pig NK lysin mutant disclosed by the invention on different bacteria, such as staphylococcus aureus, salmonella typhimurium, escherichia coli and salmonella pullorum, is enhanced.

Edible fungus solid-state fermentation feed additive based on medicinal plant forestry and agricultural residue compound matrix and preparation method of edible fungus solid-state fermentation feed additive

Resumen de: CN121196071A

The invention discloses an edible fungus solid-state fermentation feed additive based on a medicinal plant forestry and agricultural residue compound matrix and a preparation method thereof, and belongs to the technical field of microbial fermentation and feed additives, the feed additive is prepared by performing bidirectional solid-state fermentation on edible fungi by utilizing straws and a medicinal plant compound matrix, the content of antibacterial peptide is 0.8 g/kg to 3.5 g/kg, and the content of the antibacterial peptide is 0.5 g/kg to 1.5 g/kg. The straw-medicinal plant composite substrate has the advantages that the straw-medicinal plant composite substrate has the adenosine content of 0.3 g/kg to 1.8 g/kg, the crude protein content of 18% to 35% and the total polysaccharide content of 12% to 28%, and has remarkable bacteriostatic activity on escherichia coli, staphylococcus aureus and salmonella, compared with the prior art, the straw-medicinal plant composite substrate directionally induces a fungal metabolic pathway through the synergistic effect of the straw and the medicinal plant composite substrate, the content of functional active substances is greatly increased, and the application prospect is broad. The antibacterial, bacteriostatic and immune regulation functions are remarkably enhanced, the raw material cost is reduced by 60-75%, and the feed additive can be widely applied to livestock and poultry breeding as a green antibiotic replacement product.

SUBSTITUTED 2-AMINOBENZIMIDAZOLES ANALOGS AS ANTIBIOFILM AGENTS

Resumen de: US2025387373A1

In one aspect, the disclosure relates to compositions and methods for dispersing exiting Salmonella biofilms and inhibiting formation of Salmonella biofilms. In various aspects, the disclosed compositions can be used in methods of treating a persistent Salmonella infection, including an asymptomatic infection. Such infections can colonize a variety of tissues, including the gall-bladder. Also disclosed are methods of treating typhoid fever. Also disclosed are methods for mitigating or preventing secondary outbreaks of typhoid fever by treating asymptomatic subjects who had been symptomatic for typhoid fever at a previous time. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

VACCINE COMPOSITION COMPRISING A SYSTEM FOR DELIVERING AN INACTIVATED WHOLE BACTERIUM VIA CATIONIC POLYSACCHARIDE NANOPARTICLES WITHOUT ANY ADJUVANT

Resumen de: CN120712079A

The present invention relates to the field of vaccine compositions. The invention more particularly relates to a prophylactic vaccine composition comprising killed intact bacteria intended for use in mammals and birds, said bacteria being wrapped with a cationic agent, in particular cationic nanoparticles.

Primer group for simultaneously detecting four pathogenic microorganisms and application of primer group in preparation of micro-fluidic chip and kit

Resumen de: CN121182993A

The invention discloses a primer group for simultaneously detecting four pathogenic microorganisms and application of the primer group in preparation of a micro-fluidic chip and a kit. The sequences of the primers are as shown in SEQ ID NO: 1-24. The method has the advantages that the microfluidic technology and the isothermal amplification technology are combined, four sample indexes are detected at the same time in one-time detection, and the method has the advantages of being high in throughput, high in specificity, good in experiment repeatability and convenient and rapid to operate; the kit can be used for rapidly and accurately detecting salmonella (SAL), staphylococcus aureus (SA), listeria monocytogenes (LM) and escherichia coli O157: H7 (E. coli O157: H7).

System and method for risk assessment of animal population human-livestock disease

Resumen de: CN121195273A

Systems and methods for assessing the risk of a human-livestock disease, including salmonella, in an animal population at a production facility. A user may provide data in response to a plurality of data entry fields, which may relate, at least in part, to processes, operations, and/or devices at a production facility. For each data entry field, a value may be assigned to the data provided by the user. The value may correspond to a predetermined value of a predetermined response identified as corresponding to the user input data. The assigned value may be adjusted by a weighting factor, which may correspond to a predicted impact that the action or information reflected by the data input by the user may have on the prevention of zoonosis. The assigned and/or adjusted values may be compiled to generate a risk assessment score, which may be used to determine a food safety index score and/or a food safety plan.

Antibacterial peptide for inhibiting food-borne pathogens as well as acquisition method and application of antibacterial peptide

Nº publicación: CN121181665A 23/12/2025

Solicitante:

BEE RES INSTITUTE CAAS
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Resumen de: CN121181665A

The invention provides an antibacterial peptide for inhibiting food-borne pathogens as well as an acquisition method and application of the antibacterial peptide, and relates to the technical field of bioactive peptides and biomedical engineering. The amino acid sequence of the antibacterial peptide is as follows: Gly-Leu-Thr-Leu-Lys-His-Leu-Lys-Lys-Leu-Phe, and the antibacterial peptide can be used for effectively inhibiting the growth of food-borne pathogens such as staphylococcus aureus, salmonella, escherichia coli, pseudomonas aeruginosa, listeria monocytogenes and the like. The defects in the prior art are overcome, the antibacterial peptide with antibacterial activity is found and identified through a screening method combining a gastrointestinal digestive enzyme-peptide omics technology and an experiment, and a new natural antibacterial agent is provided for food, medicine and other related fields.