Microalgae: Cultivation and uses

MICROALGAE STRAIN CHLORELLA SP. VADA 2020

Absstract of: US2024287442A1

The invention relates to the field of biotechnology. A strain of microalgae Chlorella sp. VADA 2020 was isolated from water samples of Lake Tarmakul of the Chanovsky district of the Novosibirsk region and deposited in the Collection of Microalgae of the Plant Physiology Institute, RAS, under the registration number IPPAS C-2048. Available nutrient media are used for cultivating the strain. Namely, biomass with a protein content of 48.1 wt. %, and with a content of unsaturated and monounsaturated fatty acids in an amount of 59.1 wt. % (of the total content), is produced that can be used as raw materials to produce food additives. The invention makes it possible to produce biomass with a high content of protein and unsaturated fats, suitable for use for food purposes. 2 tbl, 2 ex.

RAMAN SPECTROSCOPY METHOD AND SYSTEM FOR MONITORING INVASIVE SPECIES IN ALGAL BIOREACTORS

Absstract of: US2024287436A1

A method and system for monitoring invasive species in an algal bioreactor utilizes a low resolution Raman spectrometer (LRRS) system and signal processing methods based upon Support Vector Machine (SVM) models. A spectrum preprocessing algorithm transforms LRRS spectra into normalized spectral data vectors. An ex-situ method is used to analyze a calibration set of known samples of a suspension containing a desired biomass, such as spirolina, together with various concentrations of invasive algal or cyanobacterial species. The ex-situ method generates SVM models and their associated SVM support vectors for classification modeling. An in-situ method uses the SVM models to provide an output vector of probability values corresponding to the presence of invasive species in an unknown sample, and optionally to provide an additional output vector of probability values corresponding to concentrations of the invasive species in the unknown sample.

MODIFIED STRAINS OF CHLORELLA MICROALGAE SPECIES HAVING REDUUCED CHITIN CONTENT

Absstract of: US2024287441A1

Disclosed are modified strains of Chlorella microalgae species, the modified strains having a reduced chitin content. Also disclosed is a method for producing them. The method involves performing mutagenesis of a parent strain of the microalgae. Furthermore, disclosed is a composition comprising algae biomass derived from the modified strains and their use in food and/or cosmetics amongst other applications. Advantages of the low chitin content include aiding digestibility and nutrient availability of the whole-cell ingredient without further downstream processing.

MICROALGAE-BASED PRODUCTS WITH IMPROVED NUTRIENT BIOACCESSIBILITY AND OXIDATIVE STABILITY

Absstract of: US2024287440A1

The invention relates to a method of making a product for human consumption which comprises partially lysed microalgae, said method comprising a) preparing a suspension of microalgae, wherein the microalgae belong to a phylaselected from Chlorophyta, Ochrophyta, and Heterokonta; b) applying a pulsed electric field to the suspension of microalgae, wherein said pulsed electric field has a specific energy input between 25 to 150 kJ per kg suspension (kg sus−1); c) optionally treating the microalgae with enzyme; d) incubating the biomass suspension at 300 rpm, 4-37° C. for 6-48 h e) forming a biomass of partially lysed microalgae; and f) adding the biomass of partially lysed microalgae to a product for human consumption.

CYANOBACTERIAL HOSTS AND METHODS FOR PRODUCING CHEMICALS

Absstract of: US2024279594A1

The present invention relates to recombinant cyanobacterial cells for the production of a chemical compound of interest. In particular, the present invention relates to genetic modifications that introduce one or more heterologous phosphopantetheinyl transferases (PPTases) into a cyanobacterial cell. These cells can, optionally, further comprise heterologous carrier protein and nucleic acid constructs that provide the cyanobacterial cells with the capability of producing chemicals of interest or compounds of interest, such secondary metabolites polyketides, nonribosomal peptides and their hybrids, the three major families of bioactive natural products, of cyanobacteria and other bacterial phyla, secondary metabolites analogs, and unnatural compounds.

Pharmaceutical Complex for Radioprotection, Preparation Method, and Applications thereof

Absstract of: US2024261349A1

A pharmaceutical complex for radioprotection is disclosed, as well as its preparation method and applications. Within the pharmaceutical complex, the binding between Amifostine and natural microalgae is driven by osmotic pressure. The complex is gradually degraded in the gastrointestinal tract after oral administration. The degradation of the complex makes it thoroughly cover the intestine and slowly release the drug, thereby increasing the drug concentration in the small intestine, sufficiently exerting radioprotective effects on the intestinal tissues, and avoiding systemic toxicity to the gastrointestinal tract. Additionally, the pharmaceutical complex can function as supplementary nutritional substances and regulate intestinal inflammation. The excellent oral safety, feasible preparation, simple storage, and intake of the complex prompt absorption efficiency and oral safety settle the existing issues of drug preparation technologies. The pharmaceutical complex offers wide application prospects in the field of intestinal radioprotection.

FOOD COMPOSITION

Absstract of: WO2024160738A1

The present invention relates to a food composition comprising Chlorella protein material, wherein the protein material is sourced from a chlorophyll-deficient strain of Chlorella microalgae and wherein the food composition is in the form of a meat analogue or a dry savoury snack.

FOOD COMPOSITION COMPRISING CHLOROPHYLL-DEFICIENT CHLORELLA BIOMASS WITH HIGH PROTEIN CONTENT >50WT%

Absstract of: WO2024160737A1

The present invention relates to a food composition comprising Chlorella biomass material, wherein the biomass material is sourced from a chlorophyll-deficient strain of Chlorella microalgae and wherein the Chlorella biomass material comprises at least 50 wt% of Chlorella protein by dry weight of the Chlorella biomass and wherein the food composition is in the form of a meat analogue or a dry savoury snack.

ALGAE BIOMASS

Absstract of: WO2024161109A1

The invention relates to an algae biomass having particular values within a defined colour space. The invention further relates to a strain of Chlorella microalgae having a genetic mutation in genes encoding for specific subunits of magnesium chelatase, preferably magnesium chelatase subunit, and also relates to a method of producing a strain of Chlorella microalgae having such mutations.

CULTURE SYSTEMS

Absstract of: US2024263113A1

A culture system includes a culture device that includes a culture tank for culturing microalgae, and a collection device that collects a culture solution. The culture tank includes a bottom portion located at the lowest position in the depth direction. The collection device includes a collection tube housed in the culture tank. The collection tube is a tubular body having an internal space. The collection tube includes a bottom side collection portion that extends along the bottom portion of the culture tank. The bottom side collection portion includes an open port formed at a site facing upward in the depth direction of the culture tank.

CULTURE DEVICE

Absstract of: US2024263112A1

A culture device includes an accommodation portion that accommodates a culture solution and microalgae. The accommodation portion is provided with a guide portion and a gas supply portion. The gas supply portion includes an extended tube section that extends from an upper portion to a lower portion of the guide portion in the depth direction. The extended tube portion supplies gas at the lower portion of the guide portion in the depth direction. This gas causes convection in the culture solution. The convection includes an upward flow from a lower portion to an upper portion of the accommodation portion in the depth direction. The extended tube portion is located in an upward flow region where the upward flow region is the portion through which the upward flow passes.

CHLORELLA MICROALGAE

Absstract of: WO2024161108A1

Disclosed is a chlorophyll-deficient strain of Chlorella microalgae having a protein content of at least 50% w/w. Also disclosed is a method of producing a chlorophyll-deficient strain of Chlorella microalgae having a protein content of at least 50% w/w. Also disclosed is a composition comprising an algae biomass derived from the aforementioned chlorophyll-deficient strain of Chlorella microalgae or obtained by performing aforementioned method.

GENETICALLY ENGINEERED STRAIN FOR PRODUCING POLYLACTIC ACID AND METHOD FOR PRODUCING POLYLACTIC ACID

Absstract of: EP4410984A1

Provided are a genetically engineered strain for producing polylactic acid and a method for producing polylactic acid. The method includes: synthesizing target gene using D-lactate dehydrogenase gene, propionyl-CoA transferase gene, and polyhydroxyalkanoate synthase gene; and performing double enzymatic cleavage on a vector; purifying; and transforming competent cells to obtain a recombinant plasmid; extracting DNA of the recombinant plasmid; introducing the DNA into an engineering strain, and extracting and verifying to obtain an engineering cell strain; and then culturing the engineering cell strain under light in a culture medium, in which carbon dioxide is introduced; centrifuging; drying; and recycling. According to the present application, by modifying natural cyanobacteria and using a high density fermentation strategy, the biomass of the cyanobacteria is significantly increased. According to the present application, the problem in the related art, i.e., polylactic acid can be only produced by using sugar-derived raw material, is solved.

SYSTEM AND METHOD FOR DIRECT ATMOSPHERIC CARBON CAPTURE AND MICROORGANISM CULTIVATION

Absstract of: EP4410943A1

The invention relates to a system and method for large scale direct atmospheric carbon capture (DAC) and microorganism, preferably microalgae cultivation. The system comprises an air pre-heating chamber, a bioreactor chamber containing a bulk liquid water pond containing microorganisms, which have the ability to sequester CO₂ dissolved in the water in their cells through the process of photosynthesis, preferably microalgae, and aerosolizers configured for aerosolizing water from the surface of the bulk liquid water, an air exhaust stack and a growth support system. The process comprises continuously causing ambient air to flow through the chambers, aerosolizing microorganism-containing water from the surface of the bulk liquid water pond at positions below the bioreactor inlet openings, and directing the continuous flow of heated air through the thus generated aerosol.

A composition for improving preventing and treating of bone disease comprising unsaponifiable isolated from microalgae

Absstract of: KR20240117280A

본 발명은 골질환의 개선, 예방 또는 치료용 조성물에 관한 것으로, 보다 상세하게는 미세조류 유래 불검화물을 유효성분으로 함유함으로써, 골질환을 개선, 예방 또는 치료할 수 있으므로, 식품 조성물, 나아가 건강기능식품 또는 약학 조성물로 활용될 수 있다.

a CGF a A culture method that maximizes the growth of single-celled microalgae such as Chlorella and Nannochloropsis and Efficient extraction method of natural chlorophyll a a pigment substance of microalgae and CGF a physiologically active substance and Stabilization method by coloring natural chlorophyll a in the residue remaining after extraction

Absstract of: KR20240117296A

단세포인 클로렐라, 난노클로롭시스등 미세조류를 수직관형 광배양기(도2,3)에 pH센서를 장치, 광합성량에 따라 CO2의 공급량을 자동조절하게하여 생장을 극대화(도6,7)하는 배양방법 및 미세조류의 색소물질인 천연 클로로필a와 생리활성물질인 CGF를 진공(0.1cmHg)상태에서 추출하는 방법, 추출 후 남은 미세조류 잔유물(잔사)에 진공(0.1cmHg)상태에서 천연 클로로필a를 착색시킨 후에 동결건조( >8%)한 제품을 기밀비닐스틱(도 11)에 포장, 상온에서의 8개월 이상 안정화시키는 방법.

Carotenoid production methods

Absstract of: GB2626588A

A method for producing carotenoids or pigments by culturing algae in land-based mariculture, comprises a first algal culture phase where marine carotenoid-producing microalgae are maintained in an exponential growth phase by successive dilution with seawater and nutrient addition in a series of connected raceway ponds 210. A second phase comprises inducing the algae from the first phase to produce carotenoid pigment by culturing the algae with limited nutrients, high light exposure and/or reducing the dilution rate in the raceway pond. An algal slurry is produced in a harvest stage, and the method also includes an extraction phase where the algal slurry is suspended in an alkyl-ester solvent, such as ethyl acetate, which is separated from the water phase and then evaporated, saponified and filtered. The carotenoids may be xanthophylls. A method of producing copper chlorophyllin salts is also disclosed, in which an organic non-ester is used as the extraction solvent, such as ethanol.

GEL FORMULATION FOR THE TREATMENT OF DRUG INTOXICATION AND A PROCESS TO OBTAIN THE SAME

Absstract of: EP4406532A1

The present invention relates to a gel formulation for oral administration comprising microalgae biomass from Chlorella sorokiniana, Chlorella vulgaris or Tetradesmus obliquus, free or encapsulated in particles, suitable to adsorb different drugs in the gastrointestinal tract. It is also disclosed a process to obtain the gel formulation. The gel formulation is suitable for the treatment of drug intoxication in humans or animals, therefore finding application in pharmaceutical, medical and veterinary technical fields.

USE OF CYANOBACTERIA AS A COVER CROP

Absstract of: AU2023207667A1

The subject invention provides microbial cover crops for the management of crops, soil, and water. More specifically, the invention provides for methods of using the microbe-based cover crop to address various soil quality issues, including those involving soil fertility and stability and water management. Furthermore, the microbial cover crops can be applied and maintained efficiently

CARRIERS FOR GROWING ALGAE

Absstract of: WO2024152103A1

Microalgae are grown in a biofilm on carriers. In some examples, the carriers are surfaces with a small angle to the vertical. Optionally, the carriers may have surfaces on both sides of a vertical plane (e.g., an inverted V) or surrounding a vertical axis (e.g., a cone). In some examples, the carriers include two or more sheets of a porous material such as a fabric, screen or mesh. In some examples a surface of the carrier is flexible. In some examples, the carriers provide a specific surface area for algae growth greater than 7 m2/m2.

BIODESALINATION USING MICROBIAL ATTACHED GROWTH CULTIVATION

Absstract of: US2024246844A1

A biological desalination process to remove salt from seawater using naturally grown algae or cyanobacteria in an attached growth photobioreactor setup. The photobioreactor (PBR) structure can be vertical or horizontal in orientation. Any shape of PBR can provide more light contact to the algae or cyanobacteria for enhanced growth on support media. The reactor can be designed in a way that can get maximum exposure to sunlight. The influent seawater is mixed with nutrients that are essential for the growth of algae or cyanobacteria. The main objective is to remove the salt from the brackish, saline, hypersaline or seawater. The influent seawater can be treated using the attached growth of algae or cyanobacteria, and the effluent from the PBR can be further treated based on desired water use.

MICROALGAE STRAIN HAVING EFFECT OF PROMOTING PLANT GROWTH AND USE THEREOF

Absstract of: EP4403623A1

The present application relates to a novel microalgae strain with the efficacy of promoting plant growth and a use thereof. When cultured in the presence of fermentation exhaust gas, a novel Chlorella vulgaris CD02-3002 strain according to an aspect grows fast with a high efficiency of photosynthesis and thus can effectively reduce the exhaust gas generated by microbial fermentation. Having the effect of promoting plant growth when applied to plants, a culture of the strain or a supernatant of the culture can be used as a fertilizer for plants and thus can be advantageously applied as a novel carbon reduction technique.

Self-sustaining microalgae cultivation system for the exterior wall of buildings

Absstract of: KR20240112766A

건물외벽형 미세조류 자립 배양 시스템이 제공된다. 건물외벽형 미세조류 자립 배양 시스템은 광합성을 통한 미세조류의 자립배양이 가능하도록 투명한 벽면으로 이루어지고 건물의 외벽에 설치되는 광생물 배양실을 포함하는 광생물 배양기 유닛 및 광생물 배양기 유닛으로부터 배출된 미세조류 배양액 농축액과 담수가 공급되어 배양액 농축액과 담수의 농도차에 의한 화학적 포텐셜에 의해 발전을 수행하는 염분차 발전기 유닛을 포함한다.

INFORMATION PROCESSING SYSTEM, INFORMATION PROCESSING METHOD, INFORMATION PROCESSING PROGRAM, CARBON DIOXIDE FIXATION SYSTEM, CARBON DIOXIDE FIXATION METHOD, AND CARBON DIOXIDE FIXATION PROGRAM

Absstract of: WO2024150787A1

Provided are an information processing system, an information processing method, and an information processing program that make it possible to cultivate and culture, in the same tank or bioreactor, aquatic products and microalgae, etc. on which the aquatic products feed. The present invention comprises: one culture tank in which algae and aquatic products that feed on the algae are present; a measurement unit which measures the state of the algae and/or the state of the aquatic products; a control unit which, when the measurement result requires adjustment of a culture condition, adjusts the culture condition; and a determination unit which, when the aquatic products have grown to reach a desired prescribed value, ends cultivation of the aquatic products.

NOVEL SCHIZOCHYTRIUM SP. STRAIN AND METHOD FOR PRODUCING ARACHIDONIC ACID-CONTAINING OIL USING SAME

Nº publicación: WO2024150941A1 18/07/2024

Applicant:

CJ CHEILJEDANG CORP [KR]
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