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59
results.
Updated on
15/07/2026 [08:33:00]
Results 50 to 59 of 59
Absstract of: CN121737324A
The invention relates to the technical field of medical detection, in particular to a primer group for detecting common pneumonia pathogenic bacteria of forest musk deer and application of the primer group. The invention provides a primer group for detecting common pneumonia pathogenic bacteria (escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa and pasteurella multocida) of forest musk deer, and successfully establishes a multiplex PCR (Polymerase Chain Reaction) method capable of simultaneously amplifying four pathogenic bacteria through a polymerase chain reaction principle. The method is high in specificity; the kit is high in sensitivity, and in a PCR reaction system of 25 microliters, the detection limit of escherichia coli is 9.2 * 10 < 4 > CFU/mL, the detection limit of klebsiella pneumoniae is 1.3 * 10 < 5 > CFU/mL, the detection limit of pseudomonas aeruginosa is 1.5 * 10 < 5 > CFU/mL, and the detection limit of pasteurella multocida is 3.7 * 10 < 4 > CFU/mL; clinical tests show that the method is good in repeatability.
Absstract of: CN121718619A
The invention belongs to the technical field of cosmetic detection, and particularly relates to a method, a primer group and a kit for simultaneously detecting six pathogenic bacteria in cosmetics based on a gene membrane chip technology, co-enrichment culture, genome DNA extraction, multiple PCR amplification, gene membrane chip preparation and membrane chip color development detection, and an experimental result is judged according to the actual color development condition of a target. According to the present invention, the color developing points on the membrane chip are compared with the probe layout map, the internal reference color developing shows that the bacteria are detected, the color developing of each target point represents the detection of each specific bacteria, and the detection method has characteristics of simple operation, high accuracy, good repeatability, strong specificity, high stability and high sensitivity.
Absstract of: CN121718608A
The invention provides a double probe for detecting salmonella as well as a preparation method and application of the double probe, and belongs to the technical field of bacterial detection. The double probes provided by the invention comprise an aptamer-phage-magnetic bead probe MB (at) Phage (at) Apt and an fDNA-gold nanoparticle probe AuNPs (at) fDNA; the sequence of the aptamer is as shown in SEQ ID NO: 1, and the sequence of the fDNA is as shown in SEQ ID NO: 2. The double probes are assisted by an ultraviolet lamp, on-site rapid visual detection of salmonella is realized through system color change caused by change of fluorescence intensity, the provided detection method is simple to operate, low in cost, less in time consumption and easy to realize, and on-site rapid visual detection of salmonella can be realized without large experimental equipment; a data analysis system and a linear equation are further utilized for calculation, the content of salmonella can be detected in a short time, and the detection efficiency and accuracy are improved.
Absstract of: CN121718645A
The invention discloses an Escherichia coli virulence gene and drug-resistant gene detection primer group based on time-of-flight mass spectrometry, a kit and a use method of the kit. Multiple PCR amplification primers and single-base extension primers are designed according to six virulence genes escV, stx2, hlyA, rfb, uidA and eaeA of Escherichia coli and six drug-resistant genes KPC, VIM, NDM, OXA, SHV and TEM. Wherein the nucleotide sequences of the upstream amplification primer and the downstream amplification primer of each gene are respectively shown as SEQ ID NO.1-SEQ ID NO.24, and the nucleotide sequences of the single base extension primer of each gene are respectively shown as SEQ ID NO.25-SEQ ID NO.36. According to the invention, a target product is obtained through a PCR amplification reaction; carrying out a dephosphorylation reaction and an extension reaction to obtain an extension product; and carrying out desalination treatment on the obtained extension product, carrying out sample application, and analyzing by using analysis software of a mass spectrometer. The kit has the characteristics of short period, high flux, high accuracy, better flexibility, easiness in implementation, high cost performance and the like, and provides a basis for clinical early detection of Escherichia coli infection.
Absstract of: CN121720929A
The invention discloses an integrated nano platform for bimodal detection and synergistic sterilization of pathogenic bacteria as well as a preparation method and application of the integrated nano platform, and belongs to the technical field of food safety. The platform is composed of a multifunctional nano probe (MPDA/Pd SA-DA-CDs/Apt) and a magnetic separation assembly (MNRs/PGA/AM). The probe integrates enzyme-like catalysis and photo-thermal performance and is used for generating colorimetric and photo-thermal dual-mode signals so as to realize high-sensitivity and high-reliability detection of pathogenic bacteria. The magnetic separation assembly is used for specifically capturing and enriching target bacteria and eliminating matrix interference. After detection is positive, the platform can immediately start secondary propulsion synergistic sterilization: firstly, the action distance is shortened through magnetic aggregation, and then pathogenic bacteria are efficiently inactivated and biological membranes are disintegrated by utilizing the synergistic effect of near-infrared laser excitation photothermal effect and active oxygen. According to the invention, the integration of detection and disinfection is realized, the operation is convenient, and the method has important application value in the fields of food safety and biomedicine.
Absstract of: CN121674407A
The invention provides a salmonella lateral flow chromatography biosensor. The invention particularly relates to a sandwich lateral flow chromatography sensor consisting of a nucleic acid nano enzyme and a bacteriostatic aptamer. When a target to be detected exists, the target to be detected is combined with the nucleic acid nano-enzyme probe on the combination pad, flows through the capillary action and is specifically intercepted by the Sal5-4-17nt aptamer when passing through the T line, and the redundant nucleic acid nano-enzyme probe continuously flows to the C line through the capillary action and is intercepted and captured through hybridization among nucleic acid sequences; therefore, after the color developing solution is added, obvious blue strips appear on T and C; when the to-be-detected target does not exist, the nucleic acid nano-enzyme probe is not intercepted by the T line and is intercepted only when the to-be-detected target passes through the C line, that is, only the C line has a blue band after the developing solution is added. The test paper can realize visual detection of salmonella within 5 minutes after probe dosage and hybridization sequence design and color developing solution and color developing time optimization, and meanwhile, the test paper has good biological safety.
Absstract of: CN121678796A
The invention belongs to the field of electrochemical sensors, and discloses an anti-pollution microelectrode for identifying pseudomonas aeruginosa strains and detecting phenazine spatial distribution of the pseudomonas aeruginosa strains as well as a preparation method and application of the anti-pollution microelectrode. The anti-pollution microelectrode comprises a carbon fiber electrode base body, a Fe-HHTP-PDA composite substrate layer and a Fe-HHTP-SBMA anti-pollution layer. The two-dimensional conductive metal organic framework material Fe-HHTP is doped in the anti-pollution layer based on the PDA substrate and the SBMA, so that the problem of signal attenuation caused by insulativity of the anti-pollution layer is successfully solved; by virtue of a two-dimensional ordered structure and a mixed valence iron node, the Fe-HHTP not only serves as a conductive bridge to maintain electron transmission, but also specifically catalyzes an oxidation-reduction reaction of phenazine substances, so that the anti-pollution function is realized, and meanwhile, the detection sensitivity is maintained and even improved. The preparation method is simple and convenient to operate and controllable in modification, and does not need a traditional high-pressure and high-temperature process.
Absstract of: CN121678650A
The invention provides a method for detecting residual blood through object surface disinfection wet tissue, and relates to the technical field of medical environment, medical equipment and equipment surface cleaning detection. After the object surface disinfection wet tissue with the residual blood detection function is used for collecting surface samples of a medical environment, a medical instrument and equipment, the residual blood pollution degree of the surfaces of the medical environment, the medical instrument and the equipment is qualitatively, semi-quantitatively and quantitatively judged according to the color change of the object surface disinfection wet tissue with the residual blood detection function. The semi-quantitative detection calibrator comprises multiple stages of color blocks and subdivision grids, and the residual blood volume is calculated by counting the area proportion of different color blocks through visual inspection; according to the quantitative detection AI rule, wet tissue images are scanned through photography and analysis equipment, software analyzes the mapping relation between pixel RGB values and standard concentration, the method is easy and convenient to operate, large-batch data can be rapidly processed, the cost is remarkably reduced, and the method is particularly suitable for rapid pollution evaluation of medical environments, medical instruments and equipment surfaces. Meanwhile, the pirameton can generate a composite reaction wi
Nº publicación: CN121674527A 17/03/2026
Applicant:
HUNAN KUNYUAN BIOTECHNOLOGY CO LTD
CHANGSHA ANIMAL EPIDEMIC DISEASE PREVENTION AND CONTROL CENTER
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Absstract of: CN121674527A
The invention relates to a rapid drug sensitivity detection reagent and detection method for pathogenic bacteria of aquatic animals, and the rapid drug sensitivity detection reagent for pathogenic bacteria of aquatic animals comprises the following components in percentage by weight: 1-3% of 2, 3, 5-triphenyl tetrazole chloride, 0.5-1% of iodonitrotetrazole, 5-10% of sodium chloride, 5-8% of sucrose, 0.1-0.5% of vitamin C powder and the balance of water. The invention also discloses a rapid drug sensitivity detection method of the detection reagent for pathogenic bacteria of aquatic animals. The rapid drug sensitivity detection reagent for the pathogenic bacteria of the aquatic animals is short in detection time, suitable for detection of various aquatic pathogenic bacteria, capable of supporting rapid screening of various antibiotics, low in cost and convenient to popularize and use in a large scale. The detection method is simple to operate and short in time consumption, can quickly complete drug sensitivity experiments of pathogenic bacteria of aquatic animals in batches, and is convenient for quickly and accurately screening out drugs for treating and preventing related bacterial diseases of the aquatic animals.
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