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79
results.
Updated on
15/09/2026 [09:40:00]
Absstract of: AU2026208127A1
Compositions, methods, kits, and uses are provided for detecting the presence of Salmonella, Shigella, Campylobacter, and Shigatoxigenic Escherichia coli (STEC) in samples. In some embodiments, the compositions, methods, kits, and uses comprise one or more oligonucleotides or the use of one or more oligonucleotides. ul u l
Absstract of: US20260242877A1
Disclosed herein are methods for detecting virulent Shiga toxin-producing E. coli (STEC) strains O26, O103, O121, and O111 in a biological sample comprising the steps of: (i) enriching the bacterial concentration of the biological sample to result in an enriched sample; (ii) isolating DNA from said enriched biological sample; and (iii) detecting virulent strain in said isolated DNA sample via real-time PCR and a melt curve assay. Also disclosed are primers for said assay, as well as kits comprising said primers.
Absstract of: US20260224161A1
0000 A biosensor for real-time monitoring of a wound can provide valuable data regarding the presence of bacteria. A nanofibrous biosensor is provided for monitoring wound status at the point of care. The colorimetric biosensor changes color in response to low levels of bacteria and fungi, making it visible to unaided and untrained eyes. A colorimetric probe is a hemicyanine dye that changes color from yellow to green in the presence of lipase. Dye is incorporated into a shell composition of core-shell nanofibers made of polyurethane and polyvinylpyrrolidone. As a means of increasing the biosensor's sensitivity, the alignment of nanofibers is controlled and a surfactant added to the shell (e.g., Tween 80). Alignment of nanofiber enables better localization, and Tween 80 increases lipase activity, which facilitates near immediate color changes above critical levels of Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and Candida aureus. All ESKAPEE bacteria are detected within 2 hours.
Absstract of: US20260218268A1
Disclosed are nucleic acid oligomers, including amplification oligomers and detection probes, for detection of Yersinia enterocolitica, Vibrio spp., Shiga Toxin Expressing E. coli (STEC) O157, and Plesiomonas shigelloides target nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding formulations, reaction mixtures, and kits. Methods of synthesizing the nucleic acid oligomers are also disclosed.
Absstract of: CN122466117A
The invention relates to a specific primer combination for detecting human exposed potential pathogenic bacteria in a glacier environment. The specific primer combination comprises a Klebsiella pneumoniae primer Seq1, a Pseudomonas aeruginosa primer Seq4, a Clostridium difficile primer Seq7 and a Legionella pneumophila primer Seq10, wherein the target gene of the Klebsiella pneumoniae primer Seq1 is wzc, the target gene of the Pseudomonas aeruginosa primer Seq4 is sigX, the target gene of the Clostridium difficile primer Seq7 is tcdB, and the target gene of the Legionella pneumophila primer Seq10 is mip. The method has the characteristics of high specificity and multiple targets, and can be effectively used for detection and risk identification of potential pathogenic bacteria in glacier environment samples.
Absstract of: CN122466115A
The invention provides a nucleic acid detection kit for on-site detection of multiple pathogens and application, and relates to the technical field of molecular biology. The invention provides a nucleic acid detection kit for on-site detection of multiple pathogens, which comprises a primer probe for detecting staphylococcus aureus, a primer probe for detecting pseudomonas aeruginosa, a primer probe for detecting legionella pneumophila and a primer probe for detecting haemophilus influenzae, the detection kit provided by the invention can achieve relatively high detection sensitivity to four pathogenic bacteria, and the specificity and repeatability of the detection kit both meet the detection requirements. The kit disclosed by the invention has relatively high stability and can be stored for more than one year at normal temperature.
Absstract of: CN122445823A
The invention relates to the technical field of microbiological detection, in particular to a primer probe composition and a kit for simultaneously detecting salmonella and acinetobacter baumannii and application of the primer probe composition and the kit, the primer probe composition comprises a primer pair and a probe for detecting salmonella and a primer pair and a probe for detecting acinetobacter baumannii; the nucleotide sequence of the primer pair for detecting the salmonella comprises a sequence as shown in SEQ ID NO.1-2, and the nucleotide sequence of the probe for detecting the salmonella comprises a sequence as shown in SEQ ID NO.3; the nucleotide sequence of the primer pair for detecting the acinetobacter baumannii comprises a sequence as shown in SEQ ID NO.4-5, and the nucleotide sequence of the probe for detecting the acinetobacter baumannii comprises a sequence as shown in SEQ ID NO.6. The invention further discloses a kit for detecting the acinetobacter baumannii. According to the invention, salmonella and acinetobacter baumannii can be quantitatively detected rapidly, sensitively and simultaneously in a single reaction system in a non-interfering manner, and the kit has important application value for clinical rapid screening, hospital infection control and food safety risk monitoring.
Absstract of: CN122445827A
The invention discloses a rapid detection kit for third-generation cephalosporin-resistant escherichia coli ST131 and application of the rapid detection kit, and belongs to the field of molecular biology. The invention combines an RPA isothermal amplification technology and a Cas12a/Cas13a detection technology, provides a rapid detection method for a specific fimH30 adhesin allele and a blaCTX-M drug-resistant gene of an escherichia coli ST131 pathogenic strain in a clinical sample, and realizes specific, convenient and accurate detection of pathogenic and drug-resistant escherichia coli ST131. Under the condition of constant temperature, the detection can be completed in only one hour, the sensitivity is similar to that of a qPCR method, meanwhile, the method has extremely high accuracy and convenience, complex equipment and professional operators are not needed, and on-site instant detection can be completed only through one constant temperature instrument and portable fluorescence detection equipment.
Absstract of: CN122445651A
The invention discloses a nucleic acid aptamer combination for detecting escherichia coli O157 and a biosensor of the nucleic acid aptamer combination. The nucleic acid aptamer combination for detecting the escherichia coli O157 is composed of nucleic acid aptamers shown in SEQ ID NO: 1-3, the nucleic acid aptamer combination has multiple recognition sites and can perform multi-molecule joint detection on bacterial cells to be detected through the synergistic effect, and the affinity and specificity of the aptamers to target substances are improved; when being applied to a biosensor for detection and used as an important recognition element, the Escherichia coli O157 sensor realizes high-sensitivity detection on Escherichia coli O157 in a biosensing platform, the accuracy reaches 100%, the capturing efficiency on viable bacteria cells is effectively improved, and the sensitivity of the sensor and the detection accuracy are further improved. Meanwhile, the invention provides a method for specifically detecting Escherichia coli O157 by the nucleic acid aptamer combined functionalized biosensor, and more methods and means are provided for detection of food-borne bacteria.
Absstract of: CN122445650A
The invention discloses a listeria monocytogenes nucleic acid aptamer combination with high affinity and specificity and application of the listeria monocytogenes nucleic acid aptamer combination. The nucleic acid aptamer combination obtained by research contains three specific aptamers, and the combination has multiple recognition sites and can synergistically act on specific detection of Listeria monocytogenes, so that the affinity and sensitivity of the aptamers to target substances are effectively improved; the nucleic acid aptamer is further used for preparing a biosensor, and the nucleic acid aptamer combination is modified on the sensor, so that the capturing efficiency of viable bacteria cells can be effectively improved, and the sensitivity and the detection accuracy are further improved. The optimized nucleic acid aptamer combination has high affinity and specificity and is used as an important recognition element to realize high-sensitivity detection on the listeria monocytogenes in a CNT-FET sensing platform, and the accuracy reaches 100%; and the method can also be combined with other sensing platforms to show superiority and huge potential in the aspect of efficiently diagnosing the food-borne bacteria.
Absstract of: CN122445825A
The invention relates to the technical field of microfluidics, in particular to a kit for rapidly detecting 15 respiratory tract pathogens and a use method of the kit. Comprising a plurality of reaction cavities, and primer groups corresponding to 15 respiratory tract pathogens are arranged in the reaction cavities respectively; the primer group comprises mycoplasma pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, klebsiella pneumoniae, stenotrophomonas maltophilia, haemophilus influenzae, streptococcus pneumoniae, escherichia coli, legionella pneumophila, burkholderia, staphylococcus aureus, corynebacterium striatum, aspergillus and pneumocystis yarrowii. The inner primer, the outer primer and the loop primer are used for PCR amplification of candida albicans. According to the invention, 15 respiratory tract pathogens can be detected at the same time through one-time sample adding, liquid reagents are prevented from being added into a plurality of detection tubes, and the manual operation intensity and the possible mismatching risk are reduced.
Absstract of: CN122428049A
The invention relates to the technical field of microbial molecular diagnosis, in particular to a detection platform capable of activating Cas12a trans-cleavage activity without a PAM sequence and application of the detection platform in rapid detection of aquatic pathogenic bacteria vibrio harveyi. According to the invention, the PAM dependence limitation is broken through, the efficient activation of Cas12a under the condition of no PAM sequence is realized by optimizing dsDNA target positioning and crRNA spacer region composition, the dsDNA does not need to be converted into ssDNA, and the target selection range of a CRISPR-Cas12a detection system is expanded. According to the method, the detection process is simplified, pretreatment steps such as thermal denaturation and enzymolysis are omitted, reagent types and operation links are reduced, the sample pollution risk is reduced, meanwhile, the total detection time consumption is shortened, and the method is more suitable for on-site rapid detection. The method is high in clinical application value, in clinical sample detection of trachinotus ovatus infection, the detection result of a PFCD platform is completely consistent with that of qPCR (existing molecular diagnosis'gold standard '), infected individuals can be accurately recognized, and reliable technical support is provided for early diagnosis, prevention and control of aquaculture diseases.
Absstract of: CN122404548A
The invention discloses a monoclonal antibody for detecting surface protein SapA of campylobacter fetus and application of the monoclonal antibody. The monoclonal antibodies are named as 4F6 and 7B3 respectively, and amino acid sequences of variable regions of heavy chains and light chains of the two antibodies are disclosed. The monoclonal antibody prepared by the invention can specifically recognize the surface protein SapA of the campylobacter fetus, has better specificity, and does not have cross reaction with non-campylobacter such as standard escherichia coli, salmonella, listeria, klebsiella pneumoniae, staphylococcus aureus, streptococcus and the like; no cross reaction with campylobacter jejuni, campylobacter colon and other campylobacter is generated; the test strip prepared by combining the monoclonal antibody and colloidal gold can realize on-site rapid detection of the campylobacter fetus, the detection limit is low, the sensitivity is high, and an immunological method capable of specifically detecting the campylobacter fetus is successfully developed.
Absstract of: CN122405858A
The invention relates to an oligonucleotide primer probe composition for detecting food-borne pathogenic pseudomonas bacteria (pseudomonas alcaligenes and pseudomonas buchneri) based on an ERA fluorescence method or an ERA test strip method, and a detection method using the composition. The pseudomonas alcaligenes or pseudomonas buchneri in the sample can be rapidly, specifically and sensitively detected, and the method is easy and convenient to operate, short in reaction time and free of complex temperature control equipment, and can be widely applied to rapid screening, pollution monitoring and risk early warning of food-borne pathogenic pseudomonas in various food samples such as milk and dairy products.
Absstract of: CN122405862A
The invention provides a primer for detecting Pseudomonas tolaasi, the primer comprises a forward primer and a reverse primer, the nucleotide sequence of the forward primer is as shown in SEQ ID No: 1, and the nucleotide sequence of the reverse primer is as shown in SEQ ID No: 2. The method for detecting the Pseudomonas tolaasi comprises the following steps: extracting genome DNA (Deoxyribonucleic Acid) of a sample to be detected, taking the DNA as a template, carrying out PCR (Polymerase Chain Reaction) amplification by utilizing the forward primer and the reverse primer, carrying out agarose gel electrophoresis detection on a PCR amplification product, and judging whether the sample to be detected contains the Pseudomonas tolaasi or not. The invention also provides an application of the primer for detecting the Pseudomonas tolaasi, and the primer is used for preparing a kit for detecting the Pseudomonas tolaasi, and the application of the primer for detecting the Pseudomonas tolaasi is used for detecting the Pseudomonas tolaasi. The primer for detecting Pseudomonas tolaasi, which is designed by the invention, has the advantages of strong specificity, no need of isolated culture and biochemical identification, short detection period, high efficiency, good universality and wide application prospect in allusion to a specific gene of Pseudomonas tolaasi.
Absstract of: CN122409630A
The invention discloses a salmonella dual-mode detection method based on medium-temperature Argonaute driving, and belongs to the technical field of food safety detection. According to the method, the functionalized DNA hydrogel internally packaged with an asymmetric RPA system, a CbAgo system and nano-enzyme is constructed, cascade signal amplification, DNA hydrogel phase change and nano-enzyme signal output links are integrated, and rapid detection of salmonella is realized under the assistance of gravity. The invention also independently develops portable detection equipment and an intelligent application program, realizes automatic image acquisition and analysis, has the advantages of high sensitivity, strong specificity and simplicity and convenience in operation, and is suitable for on-site rapid detection of food safety.
Absstract of: CN122405852A
The invention relates to a specific primer combination for detecting glacier melt water transmission related pathogenic microorganisms. The specific primer combination comprises a vibrio cholerae primer Seq1 with a target gene of ompW, a bacillus cereus primer Seq4 with a target gene of gyrB, a clostridium botulinum primer Seq7 with a target gene of bond/A, and a legionella pneumophila primer Seq10 with a target gene of mip. The method is high in sensitivity and good in environmental applicability, not only can realize joint detection of spreading related pathogenic microorganisms in the glacier environment, but also can be used for analyzing migration and diffusion paths in different environmental media, and provides technical support for glacier melt water spreading process and downstream biological safety risk assessment.
Absstract of: CN122405789A
The invention belongs to the technical field of biological detection, and particularly relates to a detection system for food-borne pathogenic bacteria and application of the detection system. The detection system for the food-borne pathogenic bacteria comprises a composite response material, hyaluronic acid hydrogel containing L-ascorbic acid-2-trisodium phosphate and bacteriophage, the composite response material is prepared from ZIF-90 nano particles, hyaluronidase and alkaline phosphatase, wherein the hyaluronidase and the alkaline phosphatase are encapsulated in the ZIF-90 nano particles. According to the invention, a dual-mode paper-based visual detection system based on combination of phage specific recognition and a ZIF-90 response release mechanism is constructed, and high-specificity detection of target pathogenic bacteria is realized. Compared with a nucleic acid amplification or immunological method which has the defect that live bacteria and dead bacteria are difficult to distinguish, the method provided by the invention only generates signal response to the live bacteria which can be infected by the bacteriophage, so that the authenticity and the reliability of a detection result are remarkably improved.
Absstract of: CN122405853A
The invention belongs to the technical field of biological detection, and discloses a quadruple PCR (Polymerase Chain Reaction) detection kit and method for detecting four bovine-derived respiratory tract pathogenic bacteria. The kit and the method comprise a primer pair for detecting pasteurella multocida with nucleotide sequences shown as SEQ ID NO.1 and SEQ ID NO.2, a primer pair for detecting mannheimia haemolytica with nucleotide sequences shown as SEQ ID NO.3 and SEQ ID NO.4, and a primer pair for detecting mannheimia haemolytica with nucleotide sequences shown as SEQ ID NO.3 and SEQ ID NO.4, the nucleotide sequences of the primer pair are as shown in SEQ ID NO.5 and SEQ ID NO.6, and the primer pair is used for detecting klebsiella pneumoniae; the nucleotide sequences of the primer pair are as shown in SEQ ID NO.7 and SEQ ID NO.8, and the primer pair is used for detecting mycoplasma bovis. The quadruple PCR detection kit and the quadruple PCR detection method provided by the invention can amplify DNAs of four pathogens including Pm, Mh, Kp and MB in the same reaction system, and have the characteristics of high detection efficiency, high sensitivity and strong specificity.
Absstract of: CN122385571A
The invention discloses a single-tube multiple Raman/visual detection method and kit for pathogenic bacteria based on Cas12a and an in-situ crRNA synthesis strategy, and belongs to the technical field of food safety detection. By designing single-molecule allosteric hairpin DNA containing a target recognition region, a T7 promoter and a crRNA template, 16S rRNA of specific response target bacteria, hairpin structure recombination and crRNA in-situ synthesis are started, and cis-and trans-cleavage of Cas12a is guided, so that rapid visual detection of the total bacteria amount and SERS identification and quantitative detection of different pathogenic bacteria targets are realized. The method solves the problem of insufficient qualitative accuracy and quantitative sensitivity caused by the interference of a biological matrix in the existing rapid detection method of the pathogenic bacteria, overcomes the dependence of a CRISPR/Cas12a method on a target PAM site, and greatly widens the detection range of the nucleic acid target of the food-borne pathogenic bacteria. The Raman/visual dual-mode detection system adapts to a field rapid detection scene, the detection limit is as low as the single cell level, the specificity and reproducibility are excellent, and the Raman/visual dual-mode detection system can be used for scenes such as food safety control and live pathogenic bacterium diagnosis.
Absstract of: CN122382221A
The invention relates to the technical field of nucleic acid detection, in particular to a food-borne pathogenic bacterium rapid detection method based on CRISPR-Cas12a. According to the method, after genome DNA is extracted from a food sample, bridging primers of an upstream primer combination identification gene segment and a downstream primer combination virulence gene segment are provided, the two segments are located on the same continuous DNA molecule at the interval of 50-1500 bp, and the two segments are located on the same continuous DNA molecule; amplifying for 20-40 minutes at the constant temperature of 37 DEG C, and amplifying to obtain a chimeric amplicon only when the two segments are linked to the same molecule; and activating Cas12a trans-cleavage activity by combining crRNA of a guide sequence spanning a junction site with a chimeric amplicon, cleaving a single-stranded DNA reporter probe and detecting a signal. In the single-tube constant-temperature reaction, ligase is not needed, the linkage state of the gene and the virulence gene is directly confirmed and identified, the detection limit is 10-100 CFU/mL, and the time consumed in the whole process is less than 60 minutes.
Absstract of: CN122382225A
The invention discloses a super-multiplex PCR (polymerase chain reaction) primer combination capable of simultaneously detecting 35 pathogenic bacteria and fecal indicator bacteria and application of the super-multiplex PCR primer combination. The super-multiplex PCR primer combination comprises 35 pairs of target specific primers, and nucleotide sequences of the 35 pairs of target specific primers are shown as SEQ ID NO.1-70. The method for detecting 35 pathogenic bacteria and fecal indicator bacteria comprises the following steps: extracting DNA of a sample to be detected to obtain a DNA mixture of a target sample; carrying out ultra-multiplex PCR amplification on the target sample DNA mixture to obtain a target amplification product, and carrying out high-throughput sequencing to obtain a target amplification product sequencing result; and detecting and judging the to-be-detected sample. According to the method, generation of dimers among the primers and nonspecific amplification are effectively reduced, homogenized amplification of 35 targets in a single reaction system is realized, and the method can be applied to the fields of food-borne pathogenic bacterium detection, public health emergency detection and the like.
Absstract of: CN122382222A
The invention discloses a method for rapidly detecting streptococcus suis or/and salmonella based on an RAA-EXO technology and application of the method. According to the method, specific RAA primers and EXO probes are designed and screened aiming at streptococcus suis recN genes and salmonella invA genes, the sequences of the specific RAA primers and the EXO probes are shown as SEQ ID NO.3, 5 and 10 and SEQ ID NO.14, 15 and 20 respectively, a single or double RAA-EXO detection system for the streptococcus suis and the salmonella is established, detection can be completed within 20 minutes under the condition of the constant temperature of 39-41 DEG C, and the detection sensitivity is high. And no cross reaction with common bacteria such as escherichia coli, staphylococcus aureus and the like exists. Results can be visually judged through a fluorescence curve or a blue light instrument, an effective tool is provided for rapid, sensitive and on-site detection of streptococcus suis and salmonella, and the kit has important application value in the fields of food safety and epidemic disease prevention and control.
Absstract of: CN122382226A
The invention discloses a salmonella detection system based on RPA-CRISPRCas12a and application of the salmonella detection system. The detection system comprises an RPA (recombinase polymerase amplification) primer pair, a probe, crRNA (ribonucleic acid) and LbCas12a protein, the sequences of the RPA amplification primer pair are as shown in SEQ ID NO. 1 and SEQ ID NO. 2; the sequence of the probe is as shown in SEQ ID NO.7; and the sequence of the crRNA is as shown in SEQ ID NO. 8. The RPA-CRISPR/Cas12a visual detection system has the advantages of high sensitivity, strong specificity, simplicity and convenience in operation, rapidness in detection and the like, the lowest detection limit of salmonella reaches 1 fg/mu L, and stable and repeatable fluorescence signals and visual results can still be generated under the concentration. The system effectively overcomes the defects of a traditional detection method, is suitable for on-site rapid screening and risk early warning of salmonella in a food chain, provides technical support for food safety supervision, and also provides a reference technical scheme for rapid detection of other food-borne pathogenic bacteria.
Nº publicación: CN122385882A 14/07/2026
Applicant:
GUANGDONG PHARMACEUTICAL UNIV
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Absstract of: CN122385882A
The invention provides a composite probe and a test strip for detecting food-borne pathogenic bacteria and application of the composite probe and the test strip, and belongs to the technical field of food-borne pathogenic bacteria detection. The composite probe comprises a bacteriophage functional capture probe and a ZMPAP signal composite probe; the bacteriophage functional capture probe contains a specific inactivated bacteriophage aiming at the food-borne pathogenic bacteria; the ZMPAP signal composite probe is prepared from a ZIF-67 metal organic framework material, methylene blue, polydopamine, gold nanoparticles and porcine immune globulin G. Compared with a single signal detection technology, the method disclosed by the invention has visual qualitative and accurate quantitative capabilities, is strong in specificity and good in repeatability, and provides a core technical support for on-site rapid prevention and control of the food-borne pathogenic bacteria.
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