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Processes for Producing a Fermentation Product

Absstract of: US2025313598A1

The present invention relates to polypeptides having trehalase activity, particularly derived from Talaromyces. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides for the production of ethanol.

CLOSED-LOOP SYSTEM FOR GROWTH OF AQUATIC BIOMASS AND GASIFICATION THEREOF

Absstract of: US2025313866A1

Processes, systems, and methods for producing combustible gas from wet biomass are provided. In one aspect, for example, a process for generating a combustible gas from a wet biomass in a closed system is provided. Such a process may include growing a wet biomass in a growth chamber, moving at least a portion of the wet biomass to a reactor, heating the portion of the wet biomass under high pressure in the reactor to gasify the wet biomass into a total gas component, separating the gasified component into a liquid component, a non-combustible gas component, and a combustible gas component, and introducing the liquid component and non-combustible gas component containing carbon dioxide into the growth chamber to stimulate new wet biomass growth.

NANOPARTICLES FOR THE CONTROL OF ONE-POT MULTI-ENZYMATIC REACTIONS

Absstract of: US2025313865A1

The present invention relates to a process to carry out two or more enzymatic reactions in a reaction medium, wherein the process comprises providing a system comprising at least two substantially homogeneous and colloidal populations of magnetic nanoparticles (MNPs) and applying one or more external alternating magnetic field to produce the simultaneous or sequential activation of the enzymes functionalized on the surface of each population of MNPs so that the enzymatic reactions can be thermally activated.

A HARDWOOD-DERIVED CARBOHYDRATE COMPOSITION

Absstract of: US2025313671A1

A hardwood-derived carbohydrate composition comprising 88-99.75 weight-% monomeric sugars is disclosed. The monomeric sugars include 55-85 weight-% of monomeric xylose. The carbonyl content of the carbohydrate composition is 10-1500 μg/g based on the total dry matter content of the carbohydrate composition. Further is disclosed a method for producing the hardwood-derived carbohydrate composition and the use of the same.

A MOBILE BIODIESEL MANUFACTURING PLANT FOR CONTINUOUSLY PRODUCING BIODIESEL FROM A TRIGLYCERIDE SOURCE

Absstract of: WO2025208178A1

The present invention relates to a mobile biodiesel manufacturing plant for continuously producing biodiesel from a triglyceride source and a method of continuously producing biodiesel from a triglyceride source in the mobile biodiesel manufacturing plant.

SINTERED ORE MANUFACTURING METHOD

Absstract of: EP4628605A1

Sintering materials include: a crushed product of a compression-molded product obtained by crushing the compression-molded product (S112) obtained by compressing and molding an aggregate of carbonized wood; and at least one of coke breeze or anthracite, and the compression-molded product has an envelope density of 0.6 g/cm³ or more.

IMMOBILISED LIPASE AND METHOD OF PRODUCING BIODIESEL USING THE SAME

Absstract of: WO2024119202A1

The invention relates to a lipase isolated from the goat gut rumen metagenome having protein SEQ ID 1 of 298 amino acids and being encoded for by gene SEQ ID1. The invention relates more specifically to this lipase being engineered to be an immobilised lipase, the same being immobilised on Polyethylene glycol (PEG) coated iron Magnetic Nanoparticles (MNPs). The immobilised lipase is indicated for use in a method of converting a waste oil to biodiesel, the method comprising utilizing the immobilised lipase to catalyse the conversion of said waste oils to biodiesel in the presence of a short-chain alcohol. The triacylglycerol in the waste oils being converted to alkyl esters (biodiesel) and glycerin in a reaction with an alcohol which is catalysed by said lipases.

Method for Preparing Medium-Chain Fatty Acids (MCFAs) Based on Carbon Dioxide Regulation

Absstract of: AU2025230691A1

A method for preparing medium-chain fatty acids (MCFAs) based on carbon dioxide (CO2) regulation, including: inoculating a microorganism to a substrate system to prepare MCFAs, where the microorganism is capable of producing MCFAs by carbon chain elongation (CCE); the substrate system includes an electron donor and an electron acceptor or electron acceptor precursor; and the CO2 in a reactor is controlled within a certain concentration range. A method for preparing medium-chain fatty acids (MCFAs) based on carbon dioxide (CO) regulation, including: inoculating a microorganism to a substrate system to prepare MCFAs, where the microorganism is capable of producing MCFAs by carbon chain elongation (CCE); the substrate system includes an electron donor and an electron acceptor or electron acceptor precursor; and the CO in a reactor is controlled within a certain concentration range. ep e p m e t h o d f o r p r e p a r i n g m e d i u m - c h a i n f a t t y a c i d s ( s ) b a s e d o n c a r b o n d i o x i d e ( ) r e g u l a t i o n , i n c l u d i n g : i n o c u l a t i n g a m i c r o o r g a n i s m t o a s u b s t r a t e s y s t e m t o p r e p a r e s , w h e r e t h e m i c r o o r g a n i s m i s c a p a b l e o f p r o d u c i n g s b y c a r b o n c h a i n e l o n g a t i o n ( ) ; t h e s u b s t r a t e s y s t e m i n c l u d e s a n e l e c t r o n d o n o r a n d a n e l e c t r o n a c c e p t o r o r e l e c t r o n a c c e p t o r p r e c u r s o r ; a n d

MICROBIAL FERMENTATION

Absstract of: WO2025202338A1

A microbial fermentation of a microorganism in a fermentation broth and a method of controlling said microbial fermentation is provided. The microorganism is capable of converting one or more gases into one or more organic acids and is a thermophilic microorganism. The method comprises varying the pH of the fermentation broth such as to provide a free acid concentration in the fermentation broth of greater than 8 mM; and maintaining the alcohol concentration of one or more alcohols in the fermentation broth at less than 100 mM throughout the fermentation.

METHOD FOR PREPARING KETO ACIDS, AND USE OF SAME IN PREPARATION OF AMINO ACIDS OR AMINO ACID DERIVATIVES

Absstract of: US2025305012A1

In a method for preparing a keto acid, an enzymatic reaction is carried out by using glycine and an alcoholic organic substance as substrates; the alcoholic organic substance is converted into an aldehyde organic substance, glycine and the aldehyde organic substance are converted into a β-hydroxy-α-amino acid, and then the β-hydroxy-α-amino acid is converted into a keto acid. The preparation method for a keto acid can also be used in the preparation of amino acids. The number of enzymes used is much less than that of enzymes used in a natural synthesis route, so that the production cost is low. An artificial metabolism platform for keto acids is established and can produce multiple important keto acids, such as phenylpyruvic acid, 4-methyl-2-oxopentanoic acid, pyruvic acid and 2-oxo-butyric acid.

Polypeptides Having Pullulanase Activity Suitable For Use In Liquefaction

Absstract of: US2025305006A1

The present invention relates to a variant pullulanase, having increased thermo-stability and/or increased thermo-activity compared to a parent pullulanase, comprising a substitution at least a one position selected from a position corresponding to positions 432, 486, 370, 17, 77, 103, 106, 107, 190, 196, 197, 262, 279, 283, 321, 367, 375, 382, 399, 401, 402, 411, 412, 434, 435, 443, 446, 459, 460, 479, 490, 498, 514, 529, 531, 533, 541, 545, 581, 583, 595, 649, 665, 688, 700, 709, 804, 811 of SEQ ID NO: 1, and optionally a deletion of one or more, e.g., all amino acids at positions 821, 822, 823, 824, 825, 826, 827, and 828, wherein the variant has pullulanase activity, and wherein the variant has at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to a parent alpha amylase selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5.Further aspect the present invention relates to a process for liquefying starch-containing material at a temperature above the initial gelatinization temperature using an alpha-amylase and a thermo-stable pullulanase of the invention.

RECOMBINANT YEAST STRAINS FOR PENTOSE FERMENTATION

Absstract of: EP4624568A2

Described herein are recombinant yeast cells expressing a xylulose kinase (XK) whihch are suitable for fermentation of pentoses. Also described are recombinant yeast cells with higher toleranance to formic and/or acetic acid and suitable for fermentation of pentoses. Also described are recombinant yeast cells expressing an enolase, a phophofructokinase beta subunit, a 6-phosphofructo-2-kinase, a glucose-6-phosphate isomerase, a phosphoglycerate mutase and/or a triose-phosphate isomerase, and suitable for fermentation of pentoses. Also described are recombinant yeast cells expressing a a phosphoglucomutase and/or phosphoribomutase which are suitable for fermentation of pentoses. Further described are are methods of using or producing such recombinant yeast cells and related materials.

EXPRESSION CONSTRUCTS AND METHODS OF GENETICALLY ENGINEERING METHYLOTROPHIC YEAST

Absstract of: EP4624565A2

Methods and materials for genetically engineering methylotrophic yeast are provided.

BACTERIES CLOSTRIDIUM MODIFIEES PRODUISANT DE L’ETHANOL, PREPARATION ET UTILISATIONS DE CELLES-CI

Absstract of: FR3160415A1

BACTERIES CLOSTRIDIUM MODIFIEES PRODUISANT DE L’ETHANOL, PREPARATION ET UTILISATIONS DE CELLES-CI La présente invention concerne la modification génétique de bactéries du genre Clostridium, typiquement de bactéries solvantogènes du genre Clostridium, de manière à permettre la production d’éthanol par lesdites bactéries. Sont en outre décrits des méthodes, outils et kits permettant l’obtention desdites bactéries, les bactéries génétiquement modifiées obtenues et leurs utilisations.

MODIFIED CLOSTRIDIUM BACTERIA CAPABLE OF PRODUCING ETHANOL, PREPARATION AND USES THEREOF

Absstract of: WO2025196388A1

The invention relates to the genetic modification of bacteria of the genus Clostridium, typically solventogenic bacteria of the genus Clostridium, so as to allow the production of ethanol by said bacteria. Additionally described are methods, tools and kits for obtaining said bacteria, the genetically modified bacteria obtained, and the uses of same.

METHOD FOR IMPROVING ENZYMATIC HYDROLYSIS EFFICIENCY OF LIGNOCELLULOSE

Absstract of: EP4621060A1

A method for lignocellulose hydrolysis is provided. The method includes: crushing an agroforestry biomass feedstock to a particle size of 60-80 mesh, extracting with a toluene-ethanol solution, and drying at a constant temperature to obtain a de-extracted feedstock; weighing a metal salt and glycerol with a molar ratio of 1:10-1:124 for mixing at a temperature of 80 ° C - 90 ° C and a stirring speed of 180 rpm to obtain a type IV deep eutectic solvent which is homogeneous and transparent; weighing the de-extracted feedstock and adding to the type IV deep eutectic solvent prepared, and reacting for 2-4 h at a temperature of 80 ° C - 120 ° C and a stirring speed of 300 rpm-500 rpm to obtain a pretreatment mixture; performing a solid-liquid separation to the pretreatment mixture to obtain a filter residue and a filtrate, and then drying the filter residue after washing to obtain a lignocellulose residue after pretreatment; and performing enzymatic hydrolysis reaction to the lignocellulose residue to obtain fermentable sugar.

A METHOD OF MAKING AN ETHANOL COMPOSITION AND A SEPARATE GALACTOSE COMPOSITION BY FERMENTATION

Absstract of: WO2024105280A1

A method of making an ethanol composition and a separate galactose composition by fermentation is described. The method does not require wheat substrates and does not require a distillation step to separate out the ethanol. The methods comprises treating the lactose containing substrate with a beta-galactosidase enzyme to provide an enzyme treated substrate in which at least some of the lactose in the substrate has been enzymatically converted into glucose and galactose, fermenting in a fermentation medium comprising the enzyme-treated substrate a glucose fermenting yeast to provide a fermentation broth containing ethanol and galactose, and separating the fermentation broth into an ethanol composition and a separate galactose composition. The fermentation broth is generally separated by reverse osmosis into a retentate and a permeate, in which the permeate is the ethanol composition and the retentate is the galactose composition.

一种环保型椰壳炭化炉

Absstract of: CN223373020U

本实用新型涉及椰壳炭化领域，尤其涉及一种环保型椰壳炭化炉，包括炭化箱体，炭化箱体顶部设置右排气管，炭化箱体内开设有炭化腔，炭化腔底部水平安装有椰壳放置板，椰壳放置板底部安装有过滤板，本实用通过椰壳放置板来放置需要炭化的椰壳，在椰壳炭化中，通过排液孔来排出炭化中产生的液体，设置的过滤板可以有效地过滤液体中的杂质，最后通过液体收集球收集起来，这样就可以有效地实现炭火炉在炭化椰壳时，可以有效地收集其产生的液体，后续可以对液体中的醋酸、甲醇和木焦油等进行提取，作为燃料的原料，在收集气体的同时还能收集液体，可以制备成炭火炉的燃料，减少成本，提高环保价值。

一种生物质燃烧颗粒生产用晾晒装置

Absstract of: CN223376269U

本实用新型公开了一种生物质燃烧颗粒生产用晾晒装置，涉及生物质燃烧颗粒技术领域，包括底座，所述底座上端设置有物料架，所述物料架前侧设置有C形架，所述C形架前侧设置有干燥架，所述底座内部设置有传动带，所述物料架右侧设置有第一电动机，所述第一电动机左侧设置有挡板，所述干燥架上端设置有第二电动机，所述干燥架内部设置有两组加热管，所述第二电动机下端设置有风扇。本实用新型的一种生物质燃烧颗粒生产用晾晒装置，通过设置的挡板和第一电动机等结构，第一电动机驱动挡板转动，使得挡板旋转，改变物料架出料口的出料面积，并且能够控制生物质燃烧颗粒原料每次的出料量，避免单次出料过多，原料堆积，影响晾晒效果。

一种植物纤维处理装置

Absstract of: CN223376378U

本实用新型公开了一种植物纤维处理装置：包括热源室、设于热源室内部的烘烤炉；烘烤炉呈暖水瓶胆形，烘烤炉后端固连有连接轴，连接轴连接有电动机，烘烤炉前端部与机架转动连接，热源室设有供烘烤炉炉口伸出的通孔；烘烤炉内侧壁沿其延伸方向设有螺旋叶片。本实用新型采用烘烤炉在热源室转动的形式，可以对烘烤炉内的植物原料进行烘焙、受热均匀，从而实现快速烘烤、碳化去糖，且烘烤炉内部设有螺旋叶片，烘烤炉在转动时，可以对植物原料进行翻炒，使受热更加均匀，达到植物纤维的高效分离，同时也可以保证植物原料不会从炉口漏出，安全性好，烘烤炉反转时物料即可自动排出，操作简单方便。

一种生物质原料上料装置

Absstract of: CN223366848U

本实用新型公开了一种生物质原料上料装置，包括筒体，所述筒体侧面的进料口连接有送料绞龙，所述送料绞龙的底端设置有驱动电机，所述筒体内设置有转轴和搅拌桨组成的搅拌机构，所述转轴的顶部连接有搅拌电机，所述筒体侧面设置有筛料框，所述筛料框底部设置有振动电机，且筛料框底部通过弹簧与支撑柱连接，所述筛料框的底壁表面设置有若干凸起，所述筛料框的顶壁设置有收集管，所述收集管的底部设置有多个吸附管。本实用新型结构设计科学合理，在搅拌电机的驱动下通过搅拌机构对筒体内部的物料进行搅拌，从而可以使得物料能够分散开，避免物料因潮湿而互相粘结而影响原料的后续筛选效果，提升了物料的加工质量，达到了高效上料的效果。

一种生物质颗粒筛分装置

Absstract of: CN223367442U

本实用新型公开了一种生物质颗粒筛分装置，包括可转动安装在限位圈内的筒体，所述筒体倾斜设置，所述筒体外侧固定有固定环，所述固定环外侧设置有旋转驱动机构，所述筒体内从外到内设置有第一筒型滤网和第二筒型滤网，所述筒体的端部可拆卸连接有第一接料筒、第二接料筒和第三接料筒，所述筒体的端部设置有进料口，所述进料口侧面设置有吹风机构。本实用新型结构设计科学合理，能够使得物料与筛分机构持续接触，在此过程中通过第一筒型滤网和第二筒型滤网配合对物料按照颗粒大小进行过滤处理，提升了物料的筛分处理效率。

一种生物锅炉燃料制造用挤压机进料结构

Absstract of: CN223370219U

本实用新型公开了一种生物锅炉燃料制造用挤压机进料结构，涉及生物锅炉燃料制造技术领域。本实用新型包括漏斗，漏斗上设置有防堵机构、振动机构和输料机构；防堵机构包括防堵组件和下料组件，防堵组件包括固定连接在漏斗上的连接套筒，连接套筒固定连接有双轴电机，双轴电机固定连接有第一转轴，第一转轴固定连接有连接杆，连接杆固定连接有搅拌刮板，搅拌刮板均与漏斗滑动连接，连接套筒开设有下料口。本实用新型通过防堵机构，双轴电机带动两个搅拌刮板旋转不仅将附着在漏斗内壁上的碎屑原料刮下，还可以将堆积的原料不断搅拌避免堵塞下料口，配合转动的第一螺旋叶片匀速地将原料输送进挤压机内，保证了生产效率。

一种生物质颗粒造粒机

Absstract of: CN223351610U

本实用新型属于造粒机技术领域，具体的说是一种生物质颗粒造粒机，包括底座；所述底座上部固定安装有固定座，所述挤压筒一端下方设置有挡板固定安装在底座上部，所述挡板与固定座两侧之间均固定安装有导杆，所述固定座与挡板之间设置有滑座，所述滑座滑动设置在底座上部，所述滑座上部固定安装有造粒电机，所述造粒电机转动轴与挤压绞龙一端固定连接，所述滑座下端两侧均开有导孔，所述滑座滑动安装在导杆上，所述挡板一侧开有通孔，通孔内滑动设置有拉杆，所述拉杆一端贯穿挡板且转动连接有连接杆，另一端固定连接在滑座上；通过设置拉杆用于调整滑座在导杆上的位置，从而调节挤压绞龙在挤压筒内的位置，以适应不同直径的生物质颗粒的生产需求。

一种生物质颗粒的分级筛选设备

Nº publicación: CN223352163U 19/09/2025

Applicant:

常州安斯克节能科技有限公司

Absstract of: CN223352163U

本实用新型涉及生物质颗粒领域,特别涉及一种生物质颗粒的分级筛选设备，包括箱体，所述箱体的两侧外壁均开设有出料口，且箱体内壁位于一侧的出料口处安装有筛选结构，所述箱体的两侧外壁底部处均安装有支撑组件，且箱体的一侧外壁安装有输送管，且输送管的内壁中心处转动连接有螺旋输送轴；所述输送管一侧设有添加斗；筛选结构包括安装在箱体内壁四角的连接块、安装在连接块顶部外壁的弹簧和连接在弹簧顶端外壁的筛网；支撑组件包括安装在箱体外壁的支撑块、开设在支撑块底部外壁中心处的缓冲槽和滑动连接在缓冲槽中的支撑腿。本实用新型输送电机后调档螺旋输送轴旋转，将添加斗中的物料向上输送到箱体的顶部处，物料从箱体顶部落下，便于添加物料。