Alerta

Recombinant bacillus subtilis capable of increasing pH of ruminant rumen fluid and producing compound cellulase and application thereof

Absstract of: CN120796130A

The invention relates to the technical field of microorganisms. The invention provides recombinant bacillus subtilis capable of increasing the pH value of ruminant rumen fluid and producing compound cellulase and application of the recombinant bacillus subtilis. The Latin name of the recombinant bacillus subtilis is bacillus subtilis, the preservation name is bacillus subtilis M4K2C, the preservation place is China Center for Type Culture Collection, the preservation date is August 14, 2024, and the preservation number is CCTCC NO: M20241786. When 4-5-month-old Shaanbei white cashmere goats are fed with the recombinant bacterium B.subtilis M4K2C provided by the invention for 60 days, the pH value of rumen fluid can be effectively increased. Recombinant bacteria B.subtilis M4K2C are fed to 4-5-month-old Shaanbei white cashmere goats, and relative abundance of harmful microorganism salmonella and Bild virus can be effectively inhibited by feeding the 4-5-month-old Shaanbei white cashmere goats for 30 days.

Combined medicine for treating colitis and application thereof

Absstract of: CN120789140A

The invention relates to the technical field of biological pharmacy, in particular to a combined medicine for treating colitis and application of the combined medicine, and in the combined medicine, the volume ratio of coptis chinensis exosomes to salmonella outer membrane vesicles is (1-2): (1-3); the concentration of the coptis chinensis exosome and the concentration of the salmonella outer membrane vesicles are both 9 mg/mL to 11 mg/mL. According to the application, the coptis chinensis exosome and the salmonella outer membrane vesicle are creatively combined for use, so that the limitation of a single treatment strategy is overcome, the treatment effect is remarkably improved, and the problem of side effects of an existing medicine is avoided. The traditional Chinese medicine composition is low in treatment cost, safer and free of drug resistance, and a more effective, safer and economical treatment method can be provided for inflammatory diseases such as colitis.

Preparation method of high-content cefpodoxime proxetil nanoemulsion

Absstract of: CN120788993A

The invention relates to the technical field of pharmaceutical preparations, in particular to a preparation method of a high-content cefpodoxime proxetil nanoemulsion with remarkable in-vitro antibacterial effect difference of avian salmonella and pathogenic escherichia coli. The nanoemulsion is prepared from the following raw materials in percentage by weight: 0.1%-1.5% of cefpodoxime proxetil; the oil phase is 1%-12% of origanum oil; the surfactant is prepared from 18%-25% of polyoxyethylated castor oil 40; the cosurfactant is 0.4%-5% of 1, 2-propylene glycol; and the balance of water. The advantages of the nanoemulsion and the cefpodoxime proxetil are integrated, a preliminary prescription is screened out by adopting a traditional nanoemulsion preparation method, and an optimal nanoemulsion prescription is obtained by designing and testing by adopting an L9 (34) multi-index orthogonal test method. The cefpodoxime proxetil injection has the remarkable effects that the solubility of the cefpodoxime proxetil is remarkably improved, the minimum inhibitory concentration and the minimum bactericidal concentration of the cefpodoxime proxetil to avian salmonella and pathogenic escherichia coli are remarkably reduced by using the origanum oil as the oil phase, and a pharmaceutical foundation is laid for improving the bioavailability of the cefpodoxime proxetil.

STRUCTURED MICROGEL ELECTROPHORETIC ARRAYS FOR RAPID MULTIPLEX NUCLEIC ACID DETECTION

Absstract of: US2025321205A1

Methods and devices are disclosed for rapid, multiplex molecular detection of diverse nucleic acid target molecules. The invention features an electrophoretic array with immobilized hydrogel microgel deposits. Each deposit comprises a three-dimensional, cross-linked polymer matrix containing an immobilized affinity-binding molecule and a porogen-derived pore network. This structure is configured for rapid molecular transport of nucleic acids (e.g., up to 800 bp), providing a localized environment for target capture, ligation of linear Rolling Circle Amplification (RCA) probes, and RCA. Target-specific components are anchored within distinct microgels for multiplexing. Electric fields enhance transport, reaction kinetics, and amplicon concentration. Detection is achieved in under 20 minutes. The specifically structured and fabricated microgels improve detection speed, sensitivity, and applicability to multiple different targets.

DUAL-MODE ELECTROCHEMICAL POINT-OF-CARE DETECTION, QUANTIFICATION AND PROFILING OF PATHOGENS

Absstract of: US2025321227A1

The present invention relates to diagnostic platform. More specifically, the invention relates to a dual platform composed of a detection and quantification unit and a characterization unit, systems, kits, methods and uses thereof in detection, quantification and characterization of pathogens, the system comprising monoclonal-antibody-based biosensor chips, for detection and/or quantification of pathogens in a sample, and substrate-based biosensor chips for enzymatically profiling the pathogen in the sample.

BIOMARKER COMPOSITION FOR PREDICTING HYPOPIGMENTATION KIT USING THE SAME AND INFORMATION PROVISION METHOD USING THE SAME

Absstract of: KR20250148070A

본 발명은 저색소침착증 예측용 바이오마커 조성물, 이를 이용한 키트 및 이를 이용한 정보제공방법에 관한 것으로, 보다 구체적으로는 코리네박테리움 세그토섬(Corynebacterium segmentosum), 코리네박테리움 투베르쿨로스테아리쿰(Corynebacterium tuberculostearicum), 큐티박테리움 아크네스(Cutibacterium acnes), 큐티박테리움 그래눌로섬(Cutibacterium granulosum), 로소넬라 클리블란덴시스(Lawsonella clevelandensis), Propionibacterium sp. oral taxon 193, 슈도모나스 올레오보란스(Pseudomonas oleovorans), 슈도모나스 푸티다(Pseudomonas putida), 로도코커스 sp. B7740(Rhodococcus sp. B7740), 및 스트렙토미세스 항생제(Streptomyces antibioticus)로 이루어지는 군에서 선택된 1종 이상의 미생물을 유효성분으로 포함하는 저색소침착증 예측용 바이오마커 조성물, 이를 이용한 키트 및 이를 이용한 정보제공방법에 관한 것이다.

Primer, probe and kit for constant-temperature fluorescence rapid detection of mycoplasma bovis

Absstract of: CN120776009A

The invention relates to the technical field of biological virus detection, in particular to a primer, a probe and a kit for constant-temperature fluorescence rapid detection of mycoplasma bovis. Based on a multi-enzyme constant-temperature rapid amplification technology and in combination with real-time fluorescence detection, the constant-temperature fluorescence rapid detection kit is established, the reaction is completed within 20 minutes under the constant-temperature condition of 39 DEG C, the sensitivity reaches 0.1 fg/mu L, standard strains of escherichia coli, salmonella and common golden yellow coccus and streptococcus and escherichia coli separated from cattle farms are not detected, and the specificity is high; the positive plasmid sample is repeatedly detected for 6 times, the results are consistent, and the coefficient of variation is 2.93%. The kit can be applied to rapid diagnosis and on-site monitoring of mycoplasma bovis, and provides technical support for epidemic situation control and epidemiological research.

Locusta migratoria zona pellucida membrane protein cell epitope peptide, hybridoma cell strain and anti-membrane protein epitope monoclonal antibody and application thereof

Absstract of: CN120775019A

The invention discloses a B cell epitope peptide hybridoma cell strain on migratory locust zona pellucida membrane protein LmPio and a monoclonal antibody and application thereof, LH10 is inserted into a passenger-carrying structural domain of a salmonella V-type secretion system MisL and is introduced into escherichia coli DH5alpha, the LH10 is displayed and expressed on the surface of DH5alpha thallus, the hybridoma cell strain is used as an immunogen, and the hybridoma cell strain can be used for detecting the B cell epitope peptide on the migratory locust zona pellucida membrane protein LmPio. After a mouse is immunized, splenocytes of the mouse are fused with Sp2/0 to obtain the LH10-resistant mAb hybridoma cell. The LH10 is inserted into a salmonella peg pilus operon and is introduced into an inert carrier bacterium S9H, the LH10 is subjected to functional display expression on the surface of an S9H thallus, and an S9H-peg-LH10-antibody direct mediated agglutination test established based on the display expression on the surface of the S9H thallus can specifically recognize an anti-LH10 mAb in a hybridoma cell supernatant, so that the monoclonal antibody aiming at the B cell epitope peptide LH10 on the membrane protein LmPio can be quickly screened out.

Antibacterial peptide IFD targeting gram-negative bacteria as well as preparation method and application of antibacterial peptide IFD

Absstract of: CN120775026A

The invention discloses an antibacterial peptide IFD targeting gram-negative bacteria as well as a preparation method and application thereof. The amino acid sequence of the antibacterial peptide IFD is shown as SEQ NO.1, and a C terminal is amidated by adopting-NH2. The antibacterial peptide is obtained by modifying 119-132 fragments of human-derived MD-2 protein, and is prepared by site-specific mutagenesis, hydrophobic and hydrophilic sequence insertion optimization and solid-phase chemical synthesis. The antibacterial peptide has high antibacterial activity on Gram-negative bacteria (such as escherichia coli and salmonella typhimurium), the GM Gram-negative bacteria MIC reaches 3.63 mu M and is increased by 5.24 times compared with the original peptide, the targeting property is high, and the hemolysis rate 1t when the hemolysis activity is 128 mu M is low; 3%), the Gram-negative bacteria therapeutic index is as high as 76.1, and the Gram-negative bacteria can be used as a feed antibiotic substitute for preparing drugs for treating Gram-negative bacteria infectious diseases.

Salmonella RAA fluorescent rapid detection primer, probe, detection method and application

Absstract of: CN120776011A

The invention discloses a primer, a probe and a detection method for salmonella RAA fluorescent rapid detection and application. The technical problem to be solved is to provide a salmonella detection method which is based on an RAA technology and is high in sensitivity, strong in specificity and good in repeatability. In particular discloses a composition for detecting salmonella in a tested sample, the composition comprises a pair of SA-invA-F2 and SA-invA-R1 and a probe of SA-invA-Probe, the SA-invA-F2 is a single-stranded DNA molecule of which the nucleotide sequence is SEQ ID No.4, and the SA-invA-R1 is a single-stranded DNA molecule of which the nucleotide sequence is SEQ ID No.3; a structure of the SA-invA-Probe is shown as SEQ ID No.9-tetrahydrofuran residue-SEQ ID No.10, a 26th nucleotide T of the SEQ ID No.9 is used for marking a fluorophore, a first nucleotide T of the SEQ ID No.10 is used for marking a quenching group, blocking modification is carried out on a 3'terminal, the lowest detection limit can reach 276copies/reaction and 164CFU/reaction, and the SA-invA-Probe can be used for industrial production.

Salmonella detection primer combination and detection method

Absstract of: CN120776019A

The invention discloses a salmonella detection primer combination and a detection method, and relates to the technical field of bioengineering. The primer is fimY-RPA-F3, and the sequence of the primer is as shown in SEQ ID No: 7; and the sequence of the fimY-RPA-R3 is as shown in SEQ ID No: 8. In order to establish a salmonella RPA rapid detection method, by optimizing primer design, reaction conditions and a detection process, the detection sensitivity and specificity are improved, the detection time is shortened, and the operation process is simplified, so that the requirement of on-site rapid detection is met, and a technical support is provided for food safety supervision and public health prevention and control.

一种提高重组蛋白热稳定性和溶解度的双功能蛋白标签及基于其的产品和应用

Absstract of: CN120757621A

本发明公开了一种提高重组蛋白热稳定性和溶解度的双功能蛋白标签及基于其的产品和应用，属于蛋白工程技术领域。该双功能标签的氨基酸序列SEQ ID NO:1所示，具有70%‑95%以上的相似度，其氨基酸序列变异主要存在但不限于二级结构位置。该双功能标签可在目的蛋白的N端或C端使用，适用于原核表达系统中融合蛋白的表达和纯化。本发明通过理性设计改造天然蛋白的疏水性区域，解决了现有蛋白标签分子量大、易遮蔽目标蛋白活性结构域、标签切除后残留非天然氨基酸可能破坏蛋白正确折叠等技术问题，显著提升融合蛋白在大肠杆菌表达系统中的可溶性及热稳定性，适用于包涵体蛋白的功能性表达与工业化应用。

Dual ERA-LFD kit and primer combination for visually and rapidly detecting salmonella typhimurium or single-phase variants thereof and application of dual ERA-LFD kit and primer combination

Absstract of: CN120758648A

The invention relates to a dual ERA-LFD kit for visually and rapidly detecting salmonella typhimurium or a single-phase variant thereof, a primer group and application of the dual ERA-LFD kit and the primer group, and aims at solving the problems that an existing detection method is complex in operation, long in time consumption and depends on professional equipment, and primer cross interference, high false positive rate and incapability of distinguishing the single-phase variant caused by the fact that dual ERA primer design is not standard. Screening to obtain a specific primer probe combination aiming at the fljB gene deletion region and the STM4495 gene; a dual ERA-LFD detection system is constructed, and double targets are synchronously amplified under a constant temperature condition; visual interpretation is realized through a double-detection-line color development mode of the transverse flow test strip. The detection specificity can be remarkably improved, and false positive is effectively avoided; the accurate distinguishing between the salmonella typhimurium and the single-phase variants is realized; the detection process is rapid and simple, does not need professional instruments and personnel, and is suitable for on-site rapid screening; the detection cost is obviously reduced and the basic application feasibility is improved.

サルモネラの検出方法

Absstract of: JP2025149945A

【課題】 本発明が解決しようとする課題は、サルモネラの検出を、血清型横断的かつ他の菌種とは特異的に行うことである。【解決手段】サルモネラの加熱死菌体とアジュバントを含む抗原液を、ＢＡＬＢ／ｃマウスに接種した。接種後、マウスの脾細胞またはリンパ節細胞とＰ３Ｕ１とを融合させてハイブリドーマを作製し、その中から、サルモネラ特異性を示すモノクローナルＩｇＧ抗体を産生するハイブリドーマを選抜し、優れたサルモネラ特異性抗体を産生する３つのハイブリドーマを取得することに成功した。次いで、これらのモノクローナル抗体を用いて、サンドイッチＥＬＩＳＡにより複数血清型のサルモネラおよび他の菌種の検出を行ったところ、血清型横断的にサルモネラを検出し、他の菌種を検出しないという結果を得た。【選択図】図１

Extract of herbal composition as antimicrobial and/or anti-biofilm agent

Absstract of: CN120732933A

Described herein is an extract of a herbal composition comprising at least two different dry plants, the compounds are useful as antimicrobial and/or antibiotic film agents in the treatment or prevention of microbial infections caused by bacteria (e.g., Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Streptococcus, or Staphylococcus) or by fungi, as well as in the treatment or prevention of microbial infections caused by bacteria (e.g., Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Streptococcus, or Staphylococcus). It has been found that in such an extract, the active ingredients exert their biological effects in a synergistic manner. The extracts may form active ingredients of food supplements, nutraceuticals, pharmaceutical or cosmetic compositions or functional foods or food additives. Also described herein are methods for preparing the extracts.

一种亚单位疫苗及其制备方法与应用

Absstract of: CN120737217A

本发明提供了一种亚单位疫苗及其制备方法与应用。所述疫苗包括式（1）的融合蛋白，D0a‑D1a‑Linker‑HA‑Linker‑D1b‑D0b（1）其中，D0a和D0b分别为细菌鞭毛蛋白FliC的D0结构域或其功能性片段的N端部分和C端部分，D0a和D0b组成细菌鞭毛蛋白FliC的D0结构域或其功能性片段；D1a和D1b分别为细菌鞭毛蛋白FliC的D1结构域或其功能性片段的N端部分和C端部分，D1a和D1b组成细菌鞭毛蛋白FliC的D1结构域或其功能性片段；HA为流感病毒HA蛋白的抗原表位或其功能性片段，linker为连接子，‑为肽键或其他化学键。可以用于流感病毒的净化工作，安全有效，具有较高的应用价值。

Enterococcus lactis NSK220, microbial inoculum and application

Absstract of: CN120738054A

The invention belongs to the technical field of food microorganisms, and particularly relates to an enterococcus lactis NSK220 strain, a microbial agent and application of the enterococcus lactis NSK220 strain. The invention provides the enterococcus faecium NSK220, the clearance rate of the enterococcus faecium NSK220 on DPPH is very high, the serum SOD enzyme activity can be improved by supplementing the strain NSK220 in diet, and it is indicated that the oxidation resistance of the strain NSK220 is high. Meanwhile, the enterococcus faecium NSK220 has relatively strong self-cohesion and surface hydrophobicity, which indicates that the strain has strong colonization ability and is beneficial to long-term play of probiotic functions in intestinal tracts; the strain NSK220 can also improve the feed intake and kidney function of animals, and is beneficial to the growth and body health of the animals. The enterococcus faecium NSK220 has an inhibition effect on escherichia coli, staphylococcus aureus, salmonella, pseudomonas aeruginosa and listeria monocytogenes.

FIMH INHIBITING COMPOSITIONS AND METHODS OF USE THEREOF

Absstract of: US2025304662A1

Among the various aspects of the present disclosure is the provision of FimH inhibiting compositions and methods of use thereof. FimH inhibiting compositions that target and inhibit FimH, including monoclonal antibodies, are described. Methods of identifying FimH inhibiting antibodies are also described. Further, a method of treating bacterial infections, including urinary tract infections, is described.

A PROCESS FOR PREPARING LACTOBACILLUS-BASED RECOMBINANT VACCINE CANDIDATE AGAINST MULTIPLE SALMONELLA SEROVAR IN POULTRY

Absstract of: WO2025202803A1

The process comprises providing a recombinant vaccine construct, wherein said construct comprises genetically modified Lactobacillus plantarum NC8 as a live vector; modifying the genetic structure of said Lactobacillus plantarum NC8 to express conserved Salmonella antigens, including PagN, SopE2, and FliC, anchored by a ptrk 892 backbone with a constitutive promoter, phosphoglycerate mutase (PGM); incorporating Signal Lp_2145 and cAM12 Anchor sequences into said genetic construct to enhance surface expression of recombinant proteins on Lactobacillus plantarum NC8; administering said recombinant vaccine orally to poultry, leveraging the probiotic properties of Lactobacillus plantarum NC8 for effective colonization of the poultry gastrointestinal tract; inducing a prolonged and intensified immune response by ensuring sustained high-level expression of target antigens through the utilization of the robust constitutive promoter, phosphoglycerate mutase (PGM); and optimizing immunogenicity through the surface expression of recombinant proteins on Lactobacillus plantarum NC8, fostering a robust and precisely targeted immune response.

ALTERING MICROBIAL POPULATIONS & MODIFYING MICROBIOTA

Absstract of: EP4623922A2

The invention relates to guided nucleases, CRISPR/Cas systems, crRNAs, single gRNAs, vectors, methods and pharmaceutical compositions, for example for targeting sporulating bacteria, or for targeting C difficile, Salmonella, E coli or Streptococcus.

Colloidal gold immunochromatography test paper for rapidly detecting pigeon type I paramyxovirus and preparation method of colloidal gold immunochromatography test paper

Absstract of: CN120721964A

The invention discloses colloidal gold immunochromatography test paper for rapidly detecting pigeon I-type paramyxovirus and a preparation method of the colloidal gold immunochromatography test paper. Viral diseases of pigeons, especially pigeon plague virus and pigeon adenovirus, are rapidly detected on the basis of a newly developed test strip, and the operation is simple and convenient; according to the design of antigens and antibodies of the pigeon plague virus and the pigeon adenovirus, rapid detection of the pigeon plague virus and the pigeon adenovirus can be conveniently carried out, and the obtained test strip is high in specificity and high in sensitivity. The pigeon I-type paramyxovirus detection kit has the advantages that the pigeon I-type paramyxovirus detection kit does not have cross reaction with chicken infectious bronchitis, chicken egg drop syndrome, chicken Marek's disease, avian influenza, fowl adenovirus, infectious bursal disease, escherichia coli, salmonella and other samples, but can have cross reaction with Newcastle disease, so that the pigeon I-type paramyxovirus detection kit can be used for diagnosing pigeon I-type paramyxovirus and Newcastle disease.

一种基因工程菌及其在抗革兰氏阴性菌感染中的应用

Absstract of: CN120718821A

本发明公开了一种基因工程菌及其在抗革兰氏阴性菌感染中的应用。所述基因工程菌为在出发菌株中利用细菌表面展示系统过表达菌毛黏附素、肠道消化酶识别位点和细菌素。本发明的基因工程菌能在肠道中肠激酶的作用下，释放有活性的细菌素并暴露出菌毛黏附素，并实现杀灭肠道内的鼠伤寒沙门氏菌，及预防鼠伤寒沙门氏菌的再次定植的效果。

Application of undaria pinnatifida fucoidin oligosaccharide in preparation of product for preventing/relieving intestinal inflammation induced by salmonella typhimurium

Absstract of: CN120695024A

The invention discloses application of undaria pinnatifida fucoidin oligosaccharide in preparation of a product for preventing/relieving intestinal inflammation induced by salmonella typhimurium, and belongs to the technical field of medicines. According to the application of the undaria pinnatifida fucoidin oligosaccharide in preparation of the salmonella typhimurium bacteriostatic agent, the molecular weight of the undaria pinnatifida fucoidin oligosaccharide is 4000-4500 Da, and main monosaccharides of the undaria pinnatifida fucoidin oligosaccharide are Man, Gal and Fuc. The undaria pinnatifida fucoidin oligosaccharide has a protection effect on liver and spleen organ injury, can improve the physical sign performance of the liver and spleen organ injury, relieve inflammation symptoms, effectively inhibit invasion of pathogenic bacteria, better protect colon tissues from serious damage and protect organs from pathological damage.

Salmonella enteritidis tolerant strain phage, phage preparation and application thereof

Absstract of: CN120699917A

The invention belongs to the technical field of biology, and relates to a bacteriophage, in particular to a salmonella enteritidis tolerant strain bacteriophage, a bacteriophage preparation and application of the bacteriophage preparation. The preservation number of the bacteriophage is CCTCC (China Center for Type Culture Collection) No.M 20251313, and the bacteriophage is preserved in the China Center for Type Culture Collection on June 9, 2025. The bacteriophage sBR4p4 is separated by taking a bacteriophage BS3 tolerant strain BR4 as host bacteria, and the bacteriophage is easy to separate. Biological characteristic determination results show that the sBR4p4 cracking spectrum and temperature tolerance range are wide, the stability is good, and the in-vitro cracking duration is long. Whole genome sequencing analysis shows that the bacteriophage sBR4p4 does not contain virulence genes, drug-resistant genes and lyogenic genes; and a basis is provided for further large-scale production of medicines for preventing or treating salmonella enteritidis and escherichia coli infection.

包含用于在没有任何佐剂的情况下经由阳离子多糖纳米颗粒递送灭活的完整细菌的系统的疫苗组合物

Nº publicación: CN120712079A 26/09/2025

Applicant:

瓦希纳诺公司

Absstract of: MX2025009500A

The invention relates to the field of vaccine compositions. The invention more particularly relates to a prophylactic vaccine composition that is intended for mammals and birds and comprises a killed whole bacterium, said bacterium being covered with a cationic agent, in particular cationic nanoparticles.