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Phycomycete symbiotic treatment method for purifying antibiotic resistance genes in swine wastewater

Resumen de: CN121873974A

The invention discloses a phycomycete symbiotic treatment method for purifying antibiotic resistance genes in swine wastewater, and relates to the technical field of pollution ecology, and the phycomycete symbiotic treatment method comprises the following steps: S1, expanding culture of microalgae and expanding culture domestication of fungi to obtain a microalgae expanding culture substance and a fungi expanding culture substance in a logarithmic phase; s2, establishment of a microalgae-fungus symbiont: respectively washing, precipitating and resuspending the microalgae expanding culture substance and the fungus expanding culture substance in the logarithmic phase to obtain a microalgae suspension and a fungus suspension, mixing the microalgae suspension and the fungus suspension, and performing shake cultivation for 7 days to obtain the microalgae-fungus symbiont; the temperature of the shake cultivation is 25 +/-2 DEG C, and the rotating speed is 150rpm; and S3, adding the microalgae-fungus symbiont into the swine wastewater, and alternately culturing for 10 days under light and dark conditions. According to the method, a mutualistic symbiotic relationship between microalgae-fungus communities is fully established through proper environmental parameter setting and nutrition supply, and the constructed phycomycete communities have a relatively strong synergistic effect, have a very strong capability of reducing ARGs, and are suitable for popularization.

Microalgae suspension culture system and method based on turbid circulating water carbon source

Resumen de: CN121873927A

The invention discloses a microalgae suspension culture system and method based on a turbid circulating water carbon source, and the system comprises a material supplementing unit which is used for pretreating turbid circulating water and mixing the pretreated turbid circulating water with a culture medium to obtain a carbon source adaptive culture medium in which the HCO3 <->/CO2 carbon source accounts for more than or equal to 60%; the intelligent vortex reactor is used for providing culture conditions for the carbon source adaptive culture medium and carrying out mixotrophic culture; the AI optimization reflux system is used for monitoring the microalgae density in the intelligent vortex reactor in real time and maintaining the biomass concentration in the microalgae suspension culture system by dynamically adjusting the reflux ratio based on the microalgae density monitored in real time; and the multi-mode cooperative control system is used for controlling the AI optimization backflow system to dynamically adjust the backflow proportion. By utilizing the suspension culture system, various problems of the existing suspension culture system can be solved.

Intelligent light control and nutrition circulation microalgae high-density culture equipment

Resumen de: CN121873928A

The invention relates to the technical field of microalgae culture, in particular to intelligent light control and nutrition circulation microalgae high-density culture equipment which comprises a culture tank and a tank cover detachably connected to the opening end of the culture tank, and further comprises an LED lamp panel provided with a plurality of lamp beads and arranged on one side of the culture tank; the reflecting plate is arranged on the other side of the culture tank; the light changing coating is pre-arranged on the light reflecting plate; the rotating sleeve is rotationally connected to the outer wall of the culture tank, and the LED lamp panel and the reflector are both fixedly connected to the rotating sleeve; by means of blue light emission, red light conversion and cyclic reflection, multi-layer efficient utilization of light energy is achieved, the light energy utilization rate is improved, energy consumption is reduced, meanwhile, the rotating sleeve drives the LED lamp panel and the reflector to rotate around the tank, illumination distribution in the tank is more uniform, growth difference of microalgae due to uneven illumination is effectively avoided, and the microalgae culture density and quality are improved.

METHOD FOR PRODUCING BIOMASS FROM A CYANOBACTERIA (Machine-translation by Google Translate, not legally binding)

Resumen de: ES3063464A2

The invention relates to a method for producing microalgal biomass, wherein the method comprises cultivating the microalga in a diluted seawater effluent, the microalga being selected from the group consisting of one strain of Nodularia, two strains of Nodularia, one strain of Nodularia with one strain of Chrysoreinhardia, two strains of Nodularia with one strain of Chrysoreinhardia, one strain of Nodularia with one strain of Halochlorella, two strains of Nodularia with one strain of Halochlorella, one strain of Nodularia with one strain of Halochlorella and one strain of Chrysoreinhardia, and two strains of Nodularia with one strain of Halochlorella and one strain of Chrysoreinhardia. The invention also relates to a method for remediating an effluent, wherein the method comprises cultivating a microalga of the invention in the diluted seawater effluent. (Machine-translation by Google Translate, not legally binding)

SYSTEM FOR EXTRACTION OF CARBON DIOXIDE FROM THE ATMOSPHERE AND ITS SUBSEQUENT UTILIZATION IN A MICROALGAE BIOREACTOR

Resumen de: BG5342U1

This utility model relates to a system for extracting carbon dioxide from the atmosphere and its subsequent utilization in a bioreactor with microalgae, intended for application in the field of environmental technologies and nature conservation. The created system includes a fan housing with an integrated fan (1), in which a filter cartridge with a carbon dioxide absorbent (2) is mounted. The filter cartridge with carbon dioxide absorbent (2) is connected to a compressor station (3), which is connected to a pressure vessel (4). The pressure vessel (4) is connected to a carbon dioxide mixing tank with nutrient solution (5), which is connected to the microalgae bioreactor (6). The microalgae bioreactor (6) is constructed from multiple transparent tubes to which LED lighting fixtures (7) are mounted. The microalgae bioreactor (6) is connected to a separator (8), which is connected to a biomass tank (9) and to the carbon dioxide mixing tank with nutrient solution (5).

Method for observing local death of cyanobacteria cells by using UV device

Resumen de: CN121856164A

The invention discloses a method for observing local death of cyanobacteria cells by using a UV device, and relates to the technical field of cell research, and the method comprises the following steps: culturing cyanobacteria cells to a logarithmic phase to obtain a cyanobacteria sample; preparing an observation device; dropwise adding a cyanobacteria sample to the surface of the solidified culture medium, and air-drying and fixing; locally irradiating a sample area below the opening through a UV light source; sealing the sample through a cover glass and a second silicone rubber film sheet, and transferring the marking line to the cover glass; and observing the death process of cyanobacteria cells. Local precise UV stress of cyanobacteria protonema is realized by customizing a shading plate with a local opening, in-situ long-time stable observation is guaranteed by matching agar fixation and a sealed chamber design, and a continuous fluorescence microscopic imaging technology is combined; and the accurate visual tracking of the death time sequence, spatial distribution and death signal transmission process of different cells on the single protonema is realized.

Microalgae culture carbon dioxide intelligent feeding system based on multi-parameter linkage

Resumen de: CN121852167A

The invention relates to the technical field of microalgae culture process control, in particular to a microalgae culture carbon dioxide intelligent feeding system based on multi-parameter linkage. Comprising a photobioreactor, a temperature sensor, a pH sensor, a dissolved oxygen sensor, a conductivity sensor, an optical density or turbidity sensor, a photosynthetically active radiation intensity sensor, a circulating flow sensor, a carbon dioxide and air supply module and an embedded controller. The controller sequentially completes multi-sensor access and calibration, culture operation data acquisition and calculation of growth carbon demand parameters formed by illumination, frond concentration, temperature and the like and aeration safety parameters formed by dissolved oxygen, gas-liquid ratio and circulation flow. The volume fraction of carbon dioxide, the total gas flow and the gas distribution proportion of each aeration branch are optimized by integrating the two types of parameters, and intelligent linkage control over carbon dioxide feeding in the microalgae culture process is achieved.

Microalgae incubator

Resumen de: CN224118983U

The utility model relates to a microalgae incubator which comprises a cylinder body, a guide cylinder, an air distributor and an LED (Light Emitting Diode) lamp strip, the guide cylinder is of a hollow structure and is arranged in the cylinder body, and an LED lamp strip is wound on the outer wall of the guide cylinder; the air distributor comprises a microporous aeration part and a large-aperture aeration part; a main air inlet pipe is arranged at the bottom end of the barrel, one end of the main air inlet pipe is connected with an air source, the other end of the main air inlet pipe is connected with a first air-distributing pipe and a second air-distributing pipe, the first air-distributing pipe is connected with the micropore aeration part, and the second air-distributing pipe is connected with the large-aperture aeration part. The micro-aeration holes generate fine bubbles, the large-aperture aeration holes generate large bubbles, the requirements of microalgae in different growth stages are met, the growth speed of the microalgae is increased, the large-aperture aeration holes and the micro-aeration holes are matched for use, dead zones in the barrel are reduced, the problem of blockage possibly caused by microporous aeration is avoided, and the aeration efficiency is improved. Therefore, the growth speed and quality of the microalgae are ensured.

Microalgae photoreactor capable of deeply and intelligently supplementing light and application system of microalgae photoreactor

Resumen de: CN121852171A

The invention relates to the technical field of microalgae culture, in particular to a deep intelligent light supplementing microalgae photoreactor which comprises a reactor body, a light guiding and supplementing unit, a multi-depth light intensity sensing unit, an intelligent control unit and a photovoltaic energy supply unit. The light guide element is used for efficiently conducting emergent light of the top LED light source to the middle and lower layers of a microalgae culture area in the reactor, and precise light supplement is realized by combining partition light intensity feedback and a microalgae type self-adaptive dimming strategy; the photovoltaic system provides green energy, and the dependence on a power grid is reduced. The light attenuation problem can be effectively relieved, and the light energy utilization efficiency and the microalgae culture stability are improved.

Microalgae carbonic anhydrase product, extraction method, consolidation dust suppression method and application

Resumen de: CN121852359A

The invention belongs to the cross technical field of biotechnology and environmental engineering, and discloses a microalgae carbonic anhydrase product, an extraction method, a consolidation dust suppression method and application. According to the method, the efficient consolidation and dust suppression of the sandy soil are realized by optimizing the enzyme activity and combining microorganism induced calcium carbonate precipitation (MICP) and enzyme induced calcium carbonate precipitation (EICP) technologies. The extracted carbonic anhydrase has high activity and stability, when the amount of algae liquid is 75 mL, the enzyme activity reaches up to 13.247 U/mL, and efficient catalytic performance can be kept within the wide pH value and temperature range. Application results show that the dust suppression efficiency of the microalgae carbonic anhydrase product reaches 89.853%, the evaporation rate within 10 hours is as low as 0.020 g. (m < 2 >. S) <-1 >, the water loss rate within 48 hours is only 18.835%, the wind resistance index reaches 30.986%, the dust suppression response speed is high, and the comprehensive performance is excellent.

Microalgae cell swelling inertia screening and impedance detection integrated microfluidic device

Resumen de: CN121847261A

The invention provides a microalgae cell swelling inertia screening and impedance detection integrated micro-fluidic device, and relates to the technical field of microalgae cell screening and detection. The microalgae cell swelling inertia screening and impedance detection integrated micro-fluidic device comprises a signal processing unit, the substrate is ITO (Indium Tin Oxide) conductive glass; the main micro-channel is of a micro-channel structure which is formed in a polydimethylsiloxane (PDMS) layer and is bonded on the substrate. According to the invention, the passive sorting module based on the expansion and contraction inertia effect and the differential impedance detection module are integrated in the single micro-fluidic chip, so that the full-process automation of'sample introduction-sorting-detection-output 'is realized. Sample loss, cross contamination and activity damage are avoided, interference of mixed cell populations on subsequent impedance signals is effectively eliminated through pre-sorting, and the accuracy of target microalgae detection is remarkably improved.

Preparation method and application of targeted biological bacterium and desert microalgae coupled sand stabilization composition

Resumen de: CN121825555A

The invention discloses a preparation method of a targeted biological bacterium and desert microalgae coupled sand stabilization composition. The preparation method comprises the following preparation steps: preparing a culture medium; the biomass of the fermentation liquor is larger than or equal to 2.0 * 10 < 9 > CFU.mL < 1 >, and the EPS yield of the fermentation liquor is larger than or equal to 10 g.L < 1 >. Mixing the targeted biological agent with the nostoc solution according to a volume ratio of 1: 1 to form a mixture; a synergist is added into the mixture; the invention relates to the technical field of desertification control and ecological restoration, bacteria and algae are coupled to form an initial biological membrane in a short time and then form a stable biological crust, so that wind erosion is effectively resisted; the microbial inoculum still keeps high activity under the conditions of pH 8.5 and high temperature, and is especially suitable for northwest desert environment; the organic soil conditioner in the composition can effectively reduce the soil salinity and alkalinity, form a granular structure and increase the soil organic matter content, industrial-grade raw materials and agricultural wastes are adopted, the culture medium cost is low, and the economic feasibility of large-scale application and popularization is achieved.

Culture method for remarkably improving biomass and pigment content of microalgae

Resumen de: CN121825741A

The invention discloses a culture method for remarkably improving the biomass and pigment content of microalgae, and relates to the technical field of microbial culture.The culture method comprises the following steps that tremella wastewater and pig raising wastewater are mixed according to the proportion of 1: 1-100: 1 or 1: 1-1: 100, a basic mixed solution is obtained, a composite enzymatic hydrolysate is added into the basic mixed solution, and the mixed solution is stirred to be uniform; the preparation method comprises the following steps: adding the raw materials into a stirrer, performing enzymolysis for 3-5 hours under the conditions that the temperature is 26-32 DEG C and the pH value is 6.5-7.2, standing for 2-3 hours after enzymolysis, and filtering supernate after enzymolysis by using a 0.22 mu m filter membrane to obtain a pretreatment solution. The tremella wastewater and the swine wastewater are mixed to serve as the microalgae culture medium, additional supplementation of a nitrogen source, a phosphorus source, vitamins and microelements is not needed, the problem that the cost is high due to the fact that various nutrient substances are added to traditional culture genes is solved, and agricultural and food industrial wastewater which originally easily causes water eutrophication is converted into useful resources; the economic efficiency of microalgae culture and the environmental benefit of resource utilization of wastewater are realized.

Microalgae carbon sequestration system and method

Resumen de: CN121825698A

The invention discloses a microalgae carbon sequestration system and method, and belongs to the field of carbon dioxide capture and utilization. The system comprises a natural pond, a gas supply unit, a photobioreactor, an MBR separation tank and the like, and stable operation is guaranteed through PLC automatic control and pipeline electric heat tracing. According to the method, wild type marine nannochloropsis oculata is taken as a material, CO2 gradient domestication is carried out, variable light intensity, bacteria-algae synergism and precise nutrition addition culture are combined, and then separation, concentration, dehydration and algal fat extraction are carried out to prepare the biodiesel. According to the method, the cost is reduced and the water body is purified by utilizing a natural pond water source, the microalgae carbon sequestration efficiency and biomass are remarkably improved, CO2 in a steel mill is converted into high-value biodiesel, and a low-cost and sustainable scheme is provided for carbon neutrality.

Low-energy-consumption layered bacteria-algae composite membrane aeration bio-membrane reactor system and application thereof

Resumen de: CN121823811A

The invention provides a low-energy-consumption layered bacteria-algae composite membrane aeration bio-membrane reactor system and application thereof. The system comprises a reactor body, and a membrane assembly, an oxygen supply device, a gas supply pressure monitoring device, a sewage circulating device and a light source which are arranged in the reactor body. A gas inlet pipe and a water outlet pipe are arranged at the top of the reactor body. The membrane assembly comprises a plurality of aeration membrane filaments arranged at intervals, bacterial biomembranes and microalgae biomembranes are sequentially loaded and grown on the surfaces of the aeration membrane filaments from inside to outside, and a layered composite biomembrane structure with bacteria inside and algae outside is formed. The oxygen supply device and the gas supply pressure monitoring device are communicated with the gas inlet pipe, the sewage circulating device is respectively communicated with the water inlet pipe and the water outlet pipe, and the light source is arranged outside the reactor and is used for illuminating the membrane component. The reactor has the capability of synchronously removing organic pollutants, nitrogen and phosphorus in sewage under the conditions of low aeration and even reduction or elimination of external aeration pressure. According to the layered bacteria-algae composite membrane aeration bio-membrane reactor disclosed by the invention, a stable internal bacteria-exter

Water hyacinth super-subcritical resourceful treatment method

Resumen de: CN121817319A

The invention discloses a super-subcritical resourceful treatment method for water hyacinth. Comprising the following steps: pretreating water hyacinth; the pretreated water hyacinth is mixed with a composite hydrolysis auxiliary agent composed of cyanobacteria photosensitizer and ionic carbon nanosheets and water, and the mixture is placed in a supercritical/subcritical reaction kettle to be subjected to a hydrolysis reaction; after the reaction is finished, carrying out solid-liquid separation to obtain solid residues and a liquid extract; performing anaerobic fermentation on the solid residues to prepare a feed porridge material; adding plant nutritional ingredients into the liquid extract, and adjusting the pH value to prepare the plant nutrient solution. The mass ratio of the cyanobacteria photosensitizer to the ionic carbon nanosheet is 1: (3-8). The composite hydrolysis auxiliary agent disclosed by the invention can achieve a synergistic effect, the conversion efficiency and the product quality of the water hyacinth are remarkably improved, the cellulose digestibility of a feed porridge material is improved, the effective retention rate of protein is improved, a high-quality plant nutrient solution is synchronously prepared, and efficient and high-valued resource utilization of the water hyacinth is realized.

Microalgae culture device for realizing self-adaptive supply of dissolved oxygen by using waterproof breathable membrane tube

Resumen de: CN121825711A

The invention discloses a microalgae culture device capable of realizing self-adaptive supply of dissolved oxygen by using a waterproof breathable membrane tube, a large number of micropores with pore diameters smaller than those of microalgae cells and bacteria are distributed on the tube wall of the waterproof breathable membrane tube, and the waterproof breathable membrane tube can effectively prevent bacteria in air from entering a fermentation tank, ensure a sterile environment in the tank and improve the utilization rate of the microalgae. Microalgae cells can be prevented from entering the membrane tube to cause cell loss; meanwhile, the wall surface of the waterproof and breathable membrane tube has hydrophobicity, a liquid culture medium can be effectively prevented from entering the membrane tube, a gas supply channel in the membrane tube is prevented from being blocked, and the tube wall of the waterproof and breathable membrane tube only allows gas molecules such as oxygen and carbon dioxide to freely permeate; after the air pump pressurizes ambient air, the ambient air is injected into the waterproof breathable membrane tube from the air inlet of the fermentation tank, and oxygen in the gas in the waterproof breathable membrane tube penetrates through the tube wall under the driving of concentration gradient to enter the suspension type microalgae culture system so as to provide oxygen for heterotrophic growth of microalgae cells.

Method for evaluating ecological risk of perfluorinated compound based on microalgae chlorophyll concentration

Resumen de: CN121831120A

The invention discloses a method for evaluating the ecological risk of a perfluorinated compound based on the chlorophyll concentration of microalgae, which comprises the following steps: collecting a water body of a region to be evaluated, detecting the concentration of the perfluorinated compound in the water body, and obtaining a localized microalgae culture system at the same time; adding perfluorinated compound solutions with different concentrations into the localized microalgae culture system to form an experimental group, and setting a blank group without perfluorinated compounds; sampling and detecting the chlorophyll concentration of the microalgae in the experimental group and the blank group, and calculating the chlorophyll inhibition ratio of the microalgae; calculating the half effect concentration according to the function relationship between the concentration of the perfluorinated compound and the chlorophyll inhibition ratio of the microalgae; according to the concentration and the half effect concentration of the perfluorinated compounds in the water body, calculating to obtain a risk quotient for judging and evaluating the ecological risk of the perfluorinated compounds in the water body. According to the method, the half effect concentration, the mixed exposure effect and the risk quotient value are integrated to serve as ecological risk composite criteria, and the method has the advantages of being high in environmental correlation, high in sensitivity a

Built-in LED microalgae reactor and control method thereof

Resumen de: CN121825703A

The invention relates to a built-in LED microalgae reactor and a control method thereof.The built-in LED microalgae reactor comprises a reactor cavity used for storing microalgae cell suspension liquid and a plurality of plug-in light panel structures, a plurality of mounting channels are formed in the reactor cavity, each plug-in light panel structure is inserted into any mounting channel according to needs, and each plug-in light panel structure comprises a hollow heat dissipation plate; lED lamp strips are mounted on the surfaces, corresponding to the mounting channels, of the hollow heat dissipation plate, so that the LED lamp strips are arranged internally, light energy enters an algae liquid area along the shortest path, illumination dark areas formed at the top and the bottom of a traditional light guide plate are eliminated, the light energy utilization efficiency and the light field uniformity are remarkably improved, and through the synergistic effect of the heat dissipation channels and the liquid cooling pipelines in the hollow heat dissipation plate, the light field uniformity is improved. Heat dissipation can be achieved through natural convection, circulating heat exchange can be achieved through an external liquid cooling circulating module, temperature threshold triggering type automatic heat dissipation can be achieved, light attenuation increase and light effect reduction caused by LED heat accumulation are effectively avoided, and therefore the system heat

Living paint

Resumen de: GB2701006A

A living paint comprises a living photosynthetic microorganism, binder, growth medium, and water. The microorganism may comprise algae, cyanobacteria, or lichens. The binder may be composed of primary and secondary polysaccharide binders. The primary binder may be hydroxypropyl methylcellulose and the secondary binder may be agar or sodium alginate. The growth medium may comprise phosphate, nitrate, acid (e.g. citric acid), boronic acid, or salts of magnesium, manganese, copper, or cobalt. The paint may contain a humectant, e.g. sorbitol or corn syrup. Typically, the paint comprises 1-7 wt.% binder, 40-98 wt.% water, and 0.1-6 wt.% humectant. A method of making the paint comprises the addition of the microorganism to a composition containing water and binder, which is at least partially set, wherein the growth medium is present in the composition or incorporated during/after the addition. Use of the paint for increasing carbon dioxide absorption capacity of a substrate is also disclosed. No Figure

Photosynthetic micro-ecological scaffold for repairing chronic infectious wound and preparation method thereof

Nº publicación: CN121796359A 07/04/2026

Solicitante:

THE FIRST AFFILIATED HOSPITAL OF ANHUI MEDICAL UNIV
\u5B89\u5FBD\u533B\u79D1\u5927\u5B66\u7B2C\u4E00\u9644\u5C5E\u533B\u9662

Resumen de: CN121796359A

The photosynthetic micro-ecological scaffold comprises a core layer, a shell layer and a functional coating, the core layer is a photo-crosslinking hydrogel layer loaded with photosynthetic oxygen-producing microalgae and dispersed with an inorganic carbon source, the shell layer is coated outside the core layer, and the functional coating is coated outside the shell layer. The shell layer is a structure supporting layer formed by compounding a photo-crosslinking polymer and an ionic crosslinking polymer, and the functional coating is a polydopamine layer formed on the surface of the shell layer in situ. According to the invention, a biological photosynthetic system and a physical photothermal effect are creatively and deeply fused through material and structure design. By utilizing the photothermal effect of polydopamine, two key functions of'instant antibiosis' and'promotion of microalgae carbon source supply 'are simultaneously realized, so that the two core treatment modules of antibiosis and oxygen production are no longer isolated, but form mutually-promoted positive feedback circulation, and the mutually-aggravated vicious circle of'infection' and'hypoxia 'is synergistically broken from the mechanism.