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EXTRACELLULAR VESICLES FROM GENETICALLY-MODIFIED MICROALGAE CONTAINING ENDOGENOUSLY-LOADED CARGO, THEIR PREPARATION, AND USES

NºPublicación:  US2025108080A1 03/04/2025
Solicitante: 
AGS THERAPEUTICS SAS [FR]
AGS THERAPEUTICS SAS
US_2025108080_PA

Resumen de: US2025108080A1

Provided are compositions containing extracellular vesicles (MEVs) produced in and isolated from genetically-engineered microalgae, which encode cargo, such as nucleic acids and polypeptides. The MEVs are endogenously loaded by the microalgae with the cargo. The MEVs have a variety of applications as a delivery system for therapeutics, including vaccines, anti-cancer therapeutics, diagnostics, and other such uses. The fate of MEVs upon administration depends upon the route of administration.

A METHOD FOR PREPARING AND SELECTING A POLYMERIC FLOCCULANT FOR USE IN HARVESTING MICROALGAE

NºPublicación:  AU2023350703A1 03/04/2025
Solicitante: 
UNIV OF TECHNOLOGY SYDNEY
UNIVERSITY OF TECHNOLOGY SYDNEY
AU_2023350703_A1

Resumen de: AU2023350703A1

The present invention relates to a method for harvesting microalgae, the method comprising (i) mixing one or more monomers, a photoinitiator, water and a deoxygenation system to form a mixture; (ii) exposing the mixture to UV light so as to form an aqueous solution of one or more polymers; (iii) introducing the aqueous solution of one or more polymers to a suspension comprising microalgae in an amount sufficient to produce flocculated microalgae; and (iv) harvesting the flocculated microalgae. The present invention also relates to a method for preparing and selecting a polymeric flocculant for use in harvesting microalgae and a related apparatus.

Integrated device based on microalgae culture harvesting and water circulation

NºPublicación:  CN222684635U 28/03/2025
Solicitante: 
GUIZHOU UNIV
\u8D35\u5DDE\u5927\u5B66
CN_222684635_U

Resumen de: CN222684635U

The utility model discloses an integrated device based on microalgae culture harvesting and water circulation, which comprises a microalgae culture tank, a microalgae temporary storage tank and a microalgae harvesting tank, the microalgae culture tank is fixedly arranged below the microalgae temporary storage tank, the microalgae harvesting tank is fixedly arranged on one side of the microalgae culture tank, and the microalgae harvesting tank is fixedly arranged on the other side of the microalgae culture tank. An air inlet pipe and a liquid inlet pipe are respectively mounted at the top of the microalgae temporary storage tank, a culture conveying pipe is fixedly connected between the microalgae culture tank and the microalgae temporary storage tank, a valve C is mounted on the culture conveying pipe, a harvesting conveying pipe is fixedly connected between the microalgae temporary storage tank and the microalgae harvesting tank, and a valve A is mounted on the harvesting conveying pipe. According to the microalgae culture and harvesting integrated device, microalgae culture and harvesting are integrated, the structure is simplified, the construction cost is reduced, the operation difficulty is reduced, the possibility that microalgae are polluted is reduced, and commercial production of microalgae is met.

Closed microalgae culture treatment device for pollutant treatment and carbon sequestration

NºPublicación:  CN119709361A 28/03/2025
Solicitante: 
HANGZHOU ANCIENT SHIP TECH CO LTD
ZHEJIANG GUJIA INTELLIGENT TECH CO LTD
CSSC GUJIA ZHEJIANG TECH CO LTD
\u676D\u5DDE\u53E4\u4F3D\u8239\u8236\u79D1\u6280\u6709\u9650\u516C\u53F8,
\u6D59\u6C5F\u53E4\u4F3D\u667A\u80FD\u79D1\u6280\u6709\u9650\u516C\u53F8,
\u4E2D\u8239\u53E4\u4F3D\uFF08\u6D59\u6C5F\uFF09\u79D1\u6280\u6709\u9650\u516C\u53F8
CN_119709361_PA

Resumen de: CN119709361A

The invention discloses a closed microalgae culture treatment device for pollutant treatment and carbon sequestration, and relates to the technical field of pollutant treatment.The closed microalgae culture treatment device is characterized in that a closed pipeline culture device in the prior art is modified, and adherent microalgae are cleaned by arranging a cylindrical sliding block in a tubular culture treatment unit; a plurality of annular air bags with adjustable expansion and contraction are arranged on the side wall of the cylindrical sliding block; an independent pipe body is arranged to be responsible for conveying a culture solution into the tubular culture treatment unit and outputting the culture solution from the tubular culture treatment unit; a channel for a culture solution to flow is formed in the cylindrical sliding block, and the culture solution is efficiently stirred in the moving process of the cylindrical sliding block; the closed microalgae culture treatment device for pollutant treatment and carbon sequestration has the technical effects that the cleaning strength of the closed microalgae culture treatment device for the pollutant treatment and the carbon sequestration is adjustable, the cleaning effect is stable, the microalgae culture solution is not easy to pollute during transfer, and the stirring efficiency of the microalgae solution is high.

Culture medium for realizing large-scale and low-cost microalgae growth

NºPublicación:  CN119709413A 28/03/2025
Solicitante: 
XINKE FUTURE BIO TECH XINJIANG CO LTD
\u946B\u79D1\u672A\u6765\u751F\u7269\u79D1\u6280\uFF08\u65B0\u7586\uFF09\u6709\u9650\u516C\u53F8
CN_119709413_PA

Resumen de: CN119709413A

The invention discloses a culture medium for realizing large-scale and low-cost growth of microalgae. The culture medium comprises the following raw materials: 0.25-2.5 g/L of water-soluble fertilizer; 25 to 150 mg/L of magnesium sulfate; 10 to 100 mg/L of calcium chloride; 0-80 mg/L of sodium carbonate; and trace elements. According to the culture medium, the production cost of microalgae is reduced, the stability of a formula is improved, and large-scale production in the microalgae industry can be realized, so that the biomass of various microalgae and the accumulation of bioactive substances are improved. Compared with traditional formulas such as BG11, BBM and HUT, the culture cost of the culture medium for producing 1 ton of algae powder is reduced by 30%-60% through accounting. The culture medium provided by the invention has the advantages that the growth speed of the microalgae is increased, the capability of promoting bioactive substances (grease, protein, astaxanthin and polysaccharide) synthesized by the microalgae is improved, and the culture cycle of the microalgae is shortened by 10-35%.

Agricultural microalgae fertilizer in-situ manufacturing and applying device and method

NºPublicación:  CN119709359A 28/03/2025
Solicitante: 
SHANDONG ANALYSIS AND TEST CENTER
\u5C71\u4E1C\u7701\u5206\u6790\u6D4B\u8BD5\u4E2D\u5FC3
CN_119709359_PA

Resumen de: CN119709359A

The invention discloses an agricultural microalgae fertilizer in-situ manufacturing and applying device and method, and belongs to the field of microbial fertilizer manufacturing, the device comprises a first box body, the first box body is filled with a culture solution and microalgae, the first box body is communicated with a supplement pipe, the side wall of the first box body is provided with a plurality of overflow small holes, and the overflow small holes are communicated with the supplement pipe; the supplementing pipe can supplement a culture solution into the first box body, the supplementing pipe injects the culture solution into the first box body, and when the liquid level of the culture solution reaches the overflow small hole, the culture solution containing microalgae flows out to the soil, and when injection of the culture solution into the first box body is stopped, the microalgae remaining in the first box body continues to grow; the device is easy and convenient to operate, efficient preparation and application of the microalgae fertilizer can be achieved on the premise that the activity of microalgae cells is not damaged, the manufacturing and transportation cost of the microalgae fertilizer can be greatly reduced, the utilization efficiency of the fertilizer can be improved, and healthy growth of crops can be promoted.

Method for efficiently removing nitrogen and phosphorus by using microalgae membrane reactor with alternate temperature

NºPublicación:  CN119707120A 28/03/2025
Solicitante: 
HEBEI UNIV OF TECHNOLOGY
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CN_119707120_PA

Resumen de: CN119707120A

The invention discloses a temperature-alternating microalgae membrane reactor high-efficiency nitrogen and phosphorus removal method, which comprises a reactor main body, a ceramic membrane assembly is arranged in the reactor, and microalgae are inoculated in the reactor; the reactor main body is internally provided with a stirring device for uniformly suspending microalgae in the reactor; the reactor main body is made of a transparent material, and a light source is arranged outside the reactor main body; the top of the reactor main body is communicated with a water inlet pipeline for water feeding, and the liquid level is controlled by a liquid level controller; the top of the ceramic membrane component is communicated with a water outlet pipeline; a water outlet pump is arranged on the water outlet pipeline; a temperature control device is arranged on the outer side of the reactor and can monitor, regulate and control the temperature of liquid in the reactor. According to the method, the microalgae are subjected to suspension culture in the microalgae membrane reactor in a temperature alternating manner, the sensitivity of the microalgae to the environment temperature is fully utilized, the method has the advantages of low energy consumption, few byproducts and the like, and the synchronous nitrogen and phosphorus removal efficiency of the microalgae can be remarkably improved.

Culture method of seawater microalgae

NºPublicación:  CN119709415A 28/03/2025
Solicitante: 
YUNNAN YUNCAI JINKE BIOTECHNOLOGY CO LTD
\u4E91\u5357\u4E91\u5F69\u91D1\u53EF\u751F\u7269\u6280\u672F\u6709\u9650\u516C\u53F8
CN_119709415_PA

Resumen de: CN119709415A

The invention provides a culture method of seawater microalgae. The culture method comprises the following steps: sequentially carrying out first-step culture, second-step culture, third-step culture and fourth-step culture on algae species of the seawater microalgae to obtain the seawater microalgae, 4, culturing in an outdoor pipeline-type photobioreactor, and carrying out shading treatment on the pipeline-type photobioreactor until the shading area is greater than or equal to 65%; wherein the light intensity of the shading part is 0-200 mu mol photon.m <-2 >. S <-1 >, and the light intensity of the non-shading part is 0-2000 photon.m <-2 >. S <-1 >. According to the method, the seawater microalgae can grow in a better state through a culture method of expanding a culture system step by step and finally utilizing a sealed pipeline type photobioreactor to perform fourth-step culture, and the illumination intensity at the culture stage is adjusted, so that the culture efficiency and the yield of the seawater microalgae are improved, and the yield of fucoxanthin is improved.

Desert algae sand burying preservation method

NºPublicación:  CN119709410A 28/03/2025
Solicitante: 
XINJIANG JINZHENG BIOTECHNOLOGY CO LTD
\u65B0\u7586\u91D1\u6B63\u751F\u7269\u79D1\u6280\u6709\u9650\u516C\u53F8
CN_119709410_PA

Resumen de: CN119709410A

The invention relates to a desert algae sand burying preservation method. The desert microalgae are inoculated into the sandy soil culture medium obtained by mixing the algae culture medium and the sandy soil, so that desert algae cells are preserved in the culture medium close to the original living environment, the preservation time of the desert algae cells can be effectively prolonged, the survival rate can be increased, and long-term stable preservation of the desert algae cells with specific forms and physiological characteristics can be ensured. The method is simple and rapid to operate, low in application cost and capable of keeping the original hereditary characteristics of algae species for a long time and improving the preservation effect.

EMS-ARTP compound mutation chlorella pyrenoidosa mutant strain and application thereof

NºPublicación:  CN119709412A 28/03/2025
Solicitante: 
SOUTH CHINA UNIV OF TECHNOLOGY
\u534E\u5357\u7406\u5DE5\u5927\u5B66
CN_119709412_PA

Resumen de: CN119709412A

The invention discloses an EMS-ARTP compound mutation chlorella pyrenoidosa mutant strain and an application thereof. The preparation method comprises the following steps: mutagenizing a chlorella pyrenoidosa mutant strain CX41 by adopting an ethyl methanesulfonate (EMS) and normal-pressure and room-temperature plasma (ARTP) composite technology, screening and verifying to obtain a golden yellow chlorella pyrenoidosa mutant strain, naming the golden yellow chlorella pyrenoidosa mutant strain as chlorella pyrenoidosa Y11, and screening and verifying the golden yellow chlorella pyrenoidosa Y11 to obtain the chlorella pyrenoidosa Y11. The strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on the fifth floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou on October 23, 2024, and the preservation number is GDMCC No: 65324. The chlorella pyrenoidosa mutant strain Y11 has the characteristics of high yield of protein and rich carotenoids, and can be used for fermentation production of microalgae protein and carotenoids.

Application of Synchrococcus elongatus PCC 7942

NºPublicación:  CN119709617A 28/03/2025
Solicitante: 
BEIJING INSTITUTE OF TECH
\u5317\u4EAC\u7406\u5DE5\u5927\u5B66
CN_119709617_PA

Resumen de: CN119709617A

The invention provides an application of a Synecocus elongatus PCC 7942, and relates to the technical field of biomedicine, in particular to an application of the Synecocus elongatus PCC 7942. According to the present invention, the Synchrococcus elongatus PCC 7942 belongs to cyanobacteria, and the research results of the inventor show that the strain has functions of I-type macrophage differentiation promotion, fibroblast activation inhibition and PD-L1 expression inhibition, can remodel the tumor microenvironment immunosuppression state, has good safety, and has good application prospects in tumor treatment. The invention also provides a method for inducing the Synecocus elongatus PCC 7942 to kill cancer cells, the method is simple and efficient, and the killing ability of the strain on cancer cells can be obviously improved only by performing ultrasonic treatment on the strain.

Algae-light complementary microalgae culture system and operation process thereof

NºPublicación:  CN119709367A 28/03/2025
Solicitante: 
SHANGHAI LIMING RESOURCES REUSE CO LTD
\u4E0A\u6D77\u9ECE\u660E\u8D44\u6E90\u518D\u5229\u7528\u6709\u9650\u516C\u53F8
CN_119709367_PA

Resumen de: CN119709367A

The invention provides an algae-light complementary microalgae culture system, which comprises a photovoltaic cell module, the photovoltaic cell module comprises an electric sunshade net, a storage battery and a plurality of groups of solar photovoltaic panels, and the electric sunshade net and the plurality of groups of solar photovoltaic panels are electrically connected with the storage battery; the sewage storage and transportation module is located under the electric sunshade net and electrically connected with the storage battery, and the sewage storage and transportation module is used for sewage storage, sterilization and temperature control; the microalgae culture module is located under the electric sunshade net and connected with the sewage storage and transportation module, and the microalgae culture module is used for culturing target microalgae; the remote control module is electrically connected with the electric sunshade net, the sewage storage and transportation module and the microalgae culture module. The problems that a photovoltaic power generation technology mentioned in the background technology is low in space utilization efficiency, and smoke CO2 and waste water generated by a biomass power plant are difficult to treat are solved.

Enhanced triacylglycerol productivity and extractability in engineered microalgae

NºPublicación:  CN119731197A 28/03/2025
Solicitante: 
COMMISSARIAT A LENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
UNIV JOSEPH FOURIER GRENOBLE 1
CENTRE NATIONAL DE LA RECHERCHE SCIENT
INSTITUT NATIONAL DE LA RECH AGRONOMIQUE INRA
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\u683C\u52D2\u8BFA\u5E03\u5C14\u963F\u5C14\u5351\u65AF\u5927\u5B66,
\u56FD\u5BB6\u79D1\u5B66\u7814\u7A76\u4E2D\u5FC3,
\u6CD5\u56FD\u56FD\u5BB6\u519C\u4E1A\u98DF\u54C1\u4E0E\u73AF\u5883\u7814\u7A76\u9662
CN_119731197_A

Resumen de: CN119731197A

The present invention relates to engineered single-cell native algae bacteria microalgae comprising a loss of homologous Seipin gene function, an in vitro process for the production of triacylglycerol (TAG), and uses thereof.

METHOD FOR CULTURING MICROALGAE USING AIR

NºPublicación:  WO2025063640A1 27/03/2025
Solicitante: 
KOREA UNIV RESEARCH AND BUSINESS FOUNDATION [KR]
\uACE0\uB824\uB300\uD559\uAD50 \uC0B0\uD559\uD611\uB825\uB2E8
WO_2025063640_A1

Resumen de: WO2025063640A1

The present invention relates to a method for culturing microalgae by using air. The method for culturing microalgae according to an embodiment of the present invention, in which microalgae is cultured inside a reactor, comprises: a step for adding a growth medium and limestone to the inside of the reactor; a step for supplying air to the inside of the reactor to which the growth medium and limestone were added, the air being supplied using an air pump; and a step for inoculating microalgae inside the reactor supplied with air, and growing the microalgae by performing a first photo-culture.

PLANT DISEASE RESISTANCE INDUCING AGENT, PLANT DISEASE RESISTANCE INDUCING METHOD, AND METHOD FOR PRODUCING PLANT DISEASE RESISTANCE INDUCING AGENT

NºPublicación:  US2025098687A1 27/03/2025
Solicitante: 
PANASONIC INTELLECTUAL PROPERTY MAN CO LTD [JP]
Panasonic Intellectual Property Management Co., Ltd
US_2025098687_PA

Resumen de: US2025098687A1

The present disclosure provides a plant disease resistance inducing agent that can effectively induce resistance to a plant disease. A plant disease resistance inducing agent according to the present disclosure contains a secretion product of a cyanobacterium.

MICROALGAE CULTURE APPARATUS

NºPublicación:  KR20250040844A 25/03/2025
Solicitante: 
주식회사포네이처스
WO_2025058231_PA

Resumen de: WO2025058231A1

The present invention relates to a microalgae culturing apparatus comprising: a housing which has a predetermined installation space thereinside; a culture unit which is made of a transparent material and is provided in the form of a water tank having an algae culture space filled with microalgae and a culture medium therein so as to be capable of installation in or removal from the installation space in the housing; a culture unit connection part which is provided on one side of the installation space in the housing so as to, when the culture unit is placed and installed thereon, maintain a watertight state and have a connection structure in which the inside of the culture unit and the inside of the culture unit connection part communicate with each other; a lighting part which is installed on one side of the installation space in the housing and outputs and provides light toward the culture unit when the lighting unit is driven; an air supply unit which is connected to the lower portion of the culture unit connection part and provides an air supply path for supplying air introduced into the installation space from the outside of the housing to the algae culture space in the culture unit; and a culture medium circulation unit which is connected to the lower portion of the culture unit connection part so as to suck in the microalgae and the culture medium discharged to the lower portion of the algae culture space in the culture unit and circulate and discharge the microalgae

METHOD FOR CULTURING MICROALGAE USING AIR

NºPublicación:  KR20250041590A 25/03/2025
Solicitante: 
고려대학교산학협력단

Resumen de: KR20250041590A

본 발명은 공기를 활용한 미세조류 배양방법에 관한 것으로, 본 발명의 실시예에 따른 미세조류 배양방법은 반응기의 내부에서 미세조류를 배양하는 미세조류 배양방법에 있어서, 반응기의 내부에 성장기 배지 및 라임스톤(limestone)을 첨가하는 단계, 공기펌프를 이용해 성장기 배지 및 라임스톤이 첨가된 반응기의 내부에 공기를 공급하는 단계, 및 공기가 공급된 반응기의 내부에 미세조류를 접종하고 제1 광배양하여 미세조류를 성장시키는 단계를 포함한다.

Algae-bacterium co-culture complex, culture method thereof, method for producing biomass by using algae-bacterium co-culture complex, biomass and related products

NºPublicación:  CN119685167A 25/03/2025
Solicitante: 
BINZHOU WEIQIAO GUOKE ADVANCED TECH RESEARCH INSTITUTE
BINZHOU LVDONG THERMAL POWER CO LTD
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\u6EE8\u5DDE\u7EFF\u52A8\u70ED\u7535\u6709\u9650\u516C\u53F8
CN_119685167_PA

Resumen de: CN119685167A

The invention relates to the technical field of bioengineering, and discloses an algae-bacterium co-culture complex which is obtained by co-culturing microalgae and magnetotactic bacteria. The composition of the biological hydrocarbon on the surface of the microalgae can provide a relatively anaerobic growth environment for magnetotactic bacteria, and attachment and symbiosis of the microalgae and the magnetotactic bacteria are achieved. Meanwhile, magnetosome particles, glycopeptides and other substances synthesized by magnetotactic bacteria in cells can enter microalgae cells through endocytosis to promote hydrocarbon synthesis of the microalgae cells, so that hydrocarbon accumulation of the microalgae cells is increased; magnetotactic bacteria attached to the surface and magnetosomes in algae cells endow the microalgae with the characteristics of magnetic cells, the cells can be recovered through an external magnetic field, and the magnetic separation and recovery operation is simple, the separation speed is high, the energy consumption is low, and the recovery rate is high. The invention also discloses a culture method of the algae-bacterium co-culture complex, a method for producing biomass by using the algae-bacterium co-culture complex, the biomass and related products.

Chlorella pyrenoidosa white mutant strain FZU65-W1 and application thereof

NºPublicación:  CN119685168A 25/03/2025
Solicitante: 
FUZHOU UNIV
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CN_119685168_PA

Resumen de: CN119685168A

The invention discloses a chlorella pyrenoidosa white mutant strain FZU65-W1 and application of the chlorella pyrenoidosa white mutant The classification name of the chlorella pyrenoidosa white mutant strain FZU65-W1 is chlorella pyrenoidosa, the chlorella pyrenoidosa white mutant strain is preserved in the China General Microbiological Culture Collection Center on September 11, 2024, the preservation number is CGMCC N0.46151, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. Compared with an original strain FZU65, the chlorella pyrenoidosa white mutant strain FZU65-W1 has the advantages that the growth speed of algae cells under the heterotrophic condition is higher, and meanwhile, the cells are white due to the fact that chlorophyll and carotenoid are not synthesized in the cells. The chlorella pyrenoidosa white mutant strain FZU65-W1 can be used for producing high-quality protein through heterotrophic fermentation, so that not only can a later-stage protein extraction and preparation process be simplified, but also the sensory acceptance of algae powder can be improved, and a high-quality germplasm resource is provided for industrial production and application of microalgae protein.

Ecological tail water treatment system

NºPublicación:  CN119683792A 25/03/2025
Solicitante: 
ZHEJIANG MARICULTURE RES INSTITUTE
\u6D59\u6C5F\u7701\u6D77\u6D0B\u6C34\u4EA7\u517B\u6B96\u7814\u7A76\u6240
CN_119683792_PA

Resumen de: CN119683792A

The invention discloses an ecological tail water treatment system, which comprises a culture area, a tail water storage adjusting tank, a purification area and an inlet water treatment system which are sequentially and circularly communicated, the purification area comprises a vortex separation tank, an inclined plate sedimentation tank, a brush treatment tank, a filler denitrification tank, an aeration filler tank, a secondary sedimentation tank, a salt-tolerant plant area, a microalgae purification tank and a shellfish biological filter, a filtering system convenient to replace and clean and an aeration system with a strong water stirring function are additionally arranged on a dam of the tail water storage adjusting pond, the water inlet treatment system is communicated with the breeding area, and the vortex separation pond, the inclined plate sedimentation pond, the brush treatment pond, the filler denitrification pond and the aeration filler pond are all communicated with the fermentation pond. The water inlet treatment system comprises a water inlet pipe group penetrating through the tail water storage regulating reservoir, and the water inlet pipe group exchanges heat with water in the tail water storage regulating reservoir, so that the water temperature is balanced. The ecological tail water treatment system provided by the invention can fully adjust the temperature of inlet water, and can more efficiently and fully purify the circulating water body.

Ecological treatment module of ecological tail water treatment system

NºPublicación:  CN119683774A 25/03/2025
Solicitante: 
ZHEJIANG MARICULTURE RES INSTITUTE
\u6D59\u6C5F\u7701\u6D77\u6D0B\u6C34\u4EA7\u517B\u6B96\u7814\u7A76\u6240
CN_119683774_PA

Resumen de: CN119683774A

The invention discloses an ecological treatment module of an ecological tail water treatment system, the ecological treatment module comprises a salt-tolerant surface flow wetland, a microalgae purification pond and a biological filter, supernatant water treated by a pre-treatment process is used for treating water-soluble organic matters in water through the salt-tolerant surface flow wetland, flows into the microalgae purification pond for absorbing salts in the water, and flows into the biological filter; an aeration device is arranged in the microalgae purification pond and comprises an aeration hole and an anti-blocking assembly moving relative to the surface of the aeration hole, and then the microalgae flows into the biological filter to be used for filtering and purifying the algae, so that nitrogen fixation and carbon fixation are realized. The invention provides the ecological module of the ecological tail water treatment system, which has the advantages of high efficiency, energy conservation, better treatment effect and difficulty in aeration pipe blockage.

Nitrogen-fixing cyanobacteria heteroplasm as well as rapid induction method and application thereof

NºPublicación:  CN119685196A 25/03/2025
Solicitante: 
NORTHEAST NORMAL UNIV
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CN_119685196_PA

Resumen de: CN119685196A

The invention relates to a nitrogen-fixing cyanobacteria heteromorphic cell and a rapid induction method and application thereof, and the rapid induction comprises the following steps: putting nitrogen-fixing cyanobacteria in a nitrogen-deficient culture solution under an illumination condition, inducing for 12 hours, then putting the taken nitrogen-fixing cyanobacteria in a nitrogen-deficient phosphorus-supplemented culture solution, and continuously inducing for 12 hours; meanwhile, a high-content cell imaging analysis system is used for quantifying the quantity, the cell size and the relative fluorescence intensity of the heteromorphic cells formed by differentiation; compared with induction in a culture solution which is deficient in nitrogen and not supplemented with phosphorus, the number of the heteromorphic cells of the three nitrogen-fixing blue-green algae is averagely increased by 43.3%, and the number of the heteromorphic cells of the anabaena is increased by 116%; the culture mode of nitrogen deficiency and phosphorus supply can accelerate the process of inducing heteromorphic cell differentiation, and finds that the number of the heteromorphic cells differentiated from the three strains has the best effect under the conditions of 24 hours of induction in two steps and three times of phosphorus supply.

Microalgae nutrient solution, fertilizer composition and sugar beet fertilizing method

NºPublicación:  CN119662412A 21/03/2025
Solicitante: 
INNER MONGOLIA AGRICULTURAL UNIV
\u5185\u8499\u53E4\u519C\u4E1A\u5927\u5B66
CN_119662412_PA

Resumen de: CN119662412A

The invention provides a microalgae nutrient solution and application thereof, and belongs to the technical field of agriculture. The invention provides a microalgae nutrient solution. The microalgae nutrient solution comprises nitrogen-fixing blue-green algae and photosynthetic nutrient green algae, the content of algae single cells in the microalgae nutrient solution is greater than or equal to 1.0 * 10 < 6 >. The microalgae nutrient solution provided by the invention, as a microalgae fertilizer, can significantly improve the activities of urease, sucrase and catalase in soil and reduce the activity of polyphenol oxidase in the soil; the content of alkali-hydrolyzable nitrogen in soil is increased, and the nitrogen fertilizer absorption amount of beet is improved; the richness of soil microbial communities can be increased, and the number of actinomycetes and proteobacteria is increased; growth and development of the beet can be remarkably promoted, the plant height, fresh weight, leaf number, chlorophyll content and net photosynthetic rate of the beet can be improved, accumulation of dry matter of beet tuberous roots can be promoted, timely transfer of beet growth centers can be regulated, and then the yield and sugar yield of the beet can be increased.

Preparation method and application of chitosan diatomite composite flocculant

NºPublicación:  CN119660931A 21/03/2025
Solicitante: 
GUIZHOU UNIV
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CN_119660931_PA

Resumen de: CN119660931A

The invention discloses a preparation method and application of a chitosan and diatomite composite flocculant, and the preparation method comprises the following steps: preparing a chitosan-diatomite flocculant by using chitosan and diatomite as raw materials, and then adding gamma-aminopropyltriethoxysilane (APTES) to prepare the composite flocculant (APTES-CTS/DTE). Compared with chitosan serving as a flocculating agent, the composite flocculating agent has the advantages that the use amount of the chitosan is reduced; and compared with diatomite serving as a flocculating agent, the harvesting efficiency is remarkably improved. The composite flocculant provided by the invention overcomes the defects of environmental pollution risk and influence on downstream biomass product processing due to adoption of other inorganic/organic flocculants at the present stage, and large dosage and high cost when chitosan is adopted to harvest microalgae.

Method for improving cyanobacteria harvesting efficiency based on photoinduced cell growth

Nº publicación: CN119662498A 21/03/2025

Solicitante:

INST OF HYDROBIOLOGY CHINESE ACADEMY OF SCIENCES
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CN_119662498_PA

Resumen de: CN119662498A

The invention relates to a method for harvesting liquid culture of cyanobacteria. The method comprises the step of inducing cultured cyanobacteria cells to increase the length of the cyanobacteria cells before harvesting. According to the method, a light-sensitive gene regulation and control system and a gene which is regulated and controlled by the light-sensitive gene regulation and control system and affects the cellular morphology are transferred into target cyanobacteria cells, and expression of genes related to the cellular morphology is induced through irradiation of green light, so that the cell length is successfully increased; therefore, the cultured cyanobacteria can be harvested more efficiently by using a filter membrane filtration mode.

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