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Acidochlamydomonas sp. And application thereof

Resumen de: CN122465713A

The invention relates to the technical field of microbial environmental remediation, and particularly discloses an acidophilic chlamydomonas and application thereof.Microalgae in the scheme is an acidophilic chlamydomonas sp. DY1 separated from ardealite leachate, the algae strain is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC M 2026446, and the acidophilic chlamydomonas sp. DY1 is preserved in the China Center for Type Culture Collection (CCTCC). The acidophilic chlamydomonas can grow in the ardealite leachate with the pH value of 2.0-4.0, and has a good removal effect on ammonium nitrogen and total nitrogen in the ardealite leachate; the optimal addition initial algae density of the acidophilic chlamydomonas DY1 is 1.0 * 10 < 5 >-8 * 10 < 5 > cells/mL, and the optimal treatment concentration range of the phosphogypsum leachate is that ammonium nitrogen is 25-80 mg/L, and total nitrogen is 25-80 mg/L. The method can stably remove the nitrogen-containing nutritive salt in the phosphogypsum leachate and synchronously accumulate the microalgae biomass, has the characteristics of economy, high efficiency, no secondary pollution and simplicity and convenience in operation, and is of great significance in repairing and treating the phosphogypsum leachate.

Ecological purification method for treating aquaculture wastewater based on synergy of photosynthetic bacteria and microalgae

Resumen de: CN122464580A

The invention discloses an ecological purification method for treating aquaculture wastewater based on synergy of photosynthetic bacteria and microalgae. Breeding wastewater is subjected to physical interception and electrodialysis conductance reduction pretreatment and then is introduced into the microfluidic reaction chamber, and the system alternately operates between a mass transfer mixing phase and a sensing phase and between a partition degradation phase and an optical radiation phase. In the mass transfer mixing stage, bacteria and algae are mixed and suspended for cross-boundary mass transfer, the system extracts a characteristic dielectric relaxation time constant of complex impedance data, and mode switching is triggered in combination with a preset metabolic correlation mapping model. In the partition degradation stage, the system adaptively adjusts the alternating current driving frequency, the traveling wave dielectrophoresis force is utilized to drive the microalgae and the photosynthetic bacteria to generate transverse differential separation, and a liquid phase partition is constructed; and meanwhile, the dual-frequency light source array performs phase-locked pulse radiation according to a cell space displacement track, so that independent supply of specific wavelength energy is realized. And the purified water body is subjected to split-flow liquid discharge and gravity settling to complete biomass harvesting. According to the invention, the cooperation of e

Method for rapidly forming microalgae biofilm on surface of carrier

Resumen de: CN122465906A

The invention discloses a method for rapidly forming a microalgae biological membrane on the surface of a carrier, which comprises the following steps: (1) inoculating a target microalgae species into a culture medium, and carrying out enlarged culture under proper illumination and temperature conditions to obtain an algae solution in a logarithmic phase; (2) putting a carrier material into a bio-membrane reactor, introducing the algae liquid obtained in the step (1) into the bio-membrane reactor, and submerging the carrier by the algae liquid; (3) within 0-24 hours of biofilm formation starting, enabling the water flow shear stress on the surface of the carrier to be not higher than 0.02 Pa; when the biofilm formation is started, indoleacetic acid is added into the algae liquid, so that the instantaneous concentration of the indoleacetic acid in the algae liquid reaches 0.1 mg/L; (4) further reducing the shear stress of the water flow to 0.01 Pa or below within 24-72 hours of membrane formation starting; simultaneously and respectively adding indoleacetic acid into the algae liquid at the 24th hour, the 48th hour and the 72h; (5) 72 hours after starting biofilm formation, forming pulse type water flow in the biofilm reactor by regulating and controlling the rotating speed of a driving motor, and alternately changing the water flow shear stress between 0.05 pa and 0.15 pa every 30 minutes; and continuously running under the conditions until the seventh day. According to the i

Method for enhancing carbon sequestration of microalgae symbiotic system at extreme temperature

Resumen de: CN122465712A

The invention discloses a method for enhancing carbon sequestration of a microalgae symbiotic system under heat stress, and belongs to the technical field of microalgae biological carbon sequestration. The method comprises the following specific steps: constructing a scenedesmus quadricauda and chlorella pyrenoidosa symbiotic system and inoculating the symbiotic system into a culture medium; adding exogenous betaine with the final concentration of 0.3-1.0 mM into the culture system; and introducing industrial flue gas containing carbon dioxide into the system at a heat stress temperature of 45 DEG C, and carrying out microalgae photosynthetic carbon sequestration culture under an illumination condition. The stability of the microalgae photosynthetic system at high temperature is remarkably enhanced by coupling the interspecific synergistic effect of the symbiotic microalgae with the osmotic regulation and antioxidant activation mechanism of the betaine. According to the method, the upper carbon sequestration temperature resistance limit of the microalgae is broken through to 45 DEG C, the dry weight and the carbon sequestration rate of the biomass under extreme heat stress are greatly improved, and the method is green, safe and easy for engineering amplification.

Composition containing microalga-derived extracellular vesicles

Resumen de: CN122477050A

The purpose of the present invention is to provide a composition containing extracellular vesicles derived from microalgae. In one embodiment of the present invention, a composition is prepared that contains (A) microalga-derived extracellular vesicles and one or more species selected from the group consisting of (B-1)-(B-3) (where (B-1) is one or more species selected from the group consisting of primary alcohols and polyols, (B-2) is one or more species selected from the group consisting of organic acids and salts thereof, and (B-3) is a chelating agent.

Internal light distribution photobioreactor

Resumen de: CN122465700A

An internal light distribution photobioreactor belongs to the technical field of microalgae large-scale culture related equipment, and comprises: a culture device, which is provided with a culture chamber for microalgae culture, and is of a thermal insulation structure and adjustable in culture temperature; the light gathering system is used for gathering sunlight and can provide redundant illumination for the culture device, and the light gathering ratio of the light gathering system is adjustable. According to the invention, the modular structure design of structures such as a light distribution device, a reflection system and a light condensation system can be realized. The light distribution device is innovatively arranged in the culture device, so that light distribution in the culture device can be realized, heat exchange can be realized in the culture device, and the temperature of a culture solution in the culture device can be quickly and directly adjusted. The reactor solves the problem of high difficulty in temperature adjustment and illumination intensity adjustment of the reactor in the prior art.

Microalgae lamp and culture device

Resumen de: CN224551486U

The utility model discloses a microalgae lamp and a culture device, and belongs to the technical field of microalgae culture, a light source plate is directly arranged on a heat dissipation plate, heat generated by the light source plate is directly transferred to the heat dissipation plate, and the heat dissipation area is increased due to the arrangement of heat dissipation strips, so that the heat dissipation effect is good, and the heat dissipation efficiency is high. According to the utility model, the light source plate is directly arranged on the heat dissipation plate without components such as a sealing structure and a lens, so that the manufacturing difficulty is reduced, the number of the components is reduced, the structure is simple, the light rays emitted by the lamp beads on the light source plate can directly irradiate the culture, the damage of the components such as the lens is avoided, the loss of light energy is greatly reduced, and the energy is saved. In addition, the first connecting piece is connected with one end of the heat dissipation plate, and the second connecting piece is connected with the other end of the heat dissipation plate, so that the light source mechanisms are integrated into a whole, installation and carrying are convenient, the structure is simple, and installation is easy.

Method for treating resistance genes in sewage by utilizing microalgae-fungal hypha symbiotic system and application of method

Resumen de: CN122444346A

The invention belongs to the technical field of environmental protection, and discloses an application of a microbial inoculum in treatment of sewage containing resistance genes or preparation of a product for treatment of sewage containing resistance genes, the microbial inoculum comprises seawater microalgae and fungal hyphae, the resistance genes comprise at least one of integron resistance genes, sulfonamides resistance genes, fluoroquinolones resistance genes and tetracycline resistance genes, and the salinity of the sewage is 1%-4%. According to the method for treating the sewage containing the resistance genes, the ARGs in the sewage can be effectively removed, the good removal effect on integron ARGs, sulfonamides ARGs, fluoroquinolones ARGs and tetracyclines ARGs in the sewage is achieved, and the total ARGs abundance removal rate reaches 97.66%. The method for treating the sewage containing the resistance genes is green, pollution-free and low in operation cost; operation is simple and a treatment period is short.

Yeast microorganism as well as preparation method and application thereof

Resumen de: CN122439766A

The invention relates to the technical field of microorganisms, and discloses a yeast microorganism as well as a preparation method and application thereof. The yeast microorganism comprises inactivated saccharomycetes, arginine, chitosan, trace elements and an emulsified coating layer, the emulsified coating layer coats the surface modified arginine, the chitosan and the saccharomycetes adsorbing the trace elements, and the emulsified coating layer comprises cholesterol and phospholipid. After the surfaces of the yeast microorganisms are treated by adopting chitosan and arginine, the surface potential of the yeast microorganisms is positive, trace elements can be effectively adsorbed, the yeast microorganisms are coated by phospholipid cholesterol, the average density of the yeast microorganisms is smaller than that of untreated yeast microorganisms, the suspension property of the yeast microorganisms in water can be remarkably improved, and the yeast microorganisms can be more effectively ingested and utilized by cladocerans; bad water is reduced, the dilemma that existing cladocerans culture highly depends on microalgae as bait is improved, and a basis is provided for cladocerans industrial culture.

TEXTURED FOOD PRODUCT MADE FROM MICROALGAE

Resumen de: AU2025209239A1

The invention relates to the production of a novel food product made from one or more microalgae. It can be used as an intermediate for manufacturing a more complex product intended either for human food or for veterinary food. The invention also relates to the uses of said food product made from one or more microalgae. The invention also relates to a method for producing said food product made from one or more microalgae.

LIPID PRODUCING MARINE MICROALGA

Resumen de: US20260209683A1

0000 The invention provides marine microalgal strain of the Ulvales order of the Ulvophyceae class which have utility in the efficient and environmentally beneficial preparation of triacylglycerides and polyunsaturated fatty acids, which can in turn be used for fuels or feeds.

High-sensitivity and high-efficiency detection method for number of gametes of noctiluca noctiluca

Resumen de: CN122428024A

The invention relates to a high-sensitivity and high-efficiency method for detecting the number of gametes of noctiluca, which comprises the following steps: filtering a water sample to be detected, collecting microalgae cells, and extracting by adopting a Chelex-100 chelation method to obtain a DNA (Deoxyribose Nucleic Acid) template; a ddPCR reaction system is constructed, the ddPCR reaction system comprises the DNA template, a specific primer pair and a TaqMan probe, the sequence of the specific primer pair is an Ns175F primer as shown in SEQ ID NO.1 and an Ns268R primer as shown in SEQ ID NO.2, the sequence of the TaqMan probe is as shown in SEQ ID NO.3, the 5'end of the probe is labeled with a fluorescence reporter group, and the 3 'end of the probe is labeled with a fluorescence quenching group; carrying out PCR amplification on the ddPCR reaction system after the ddPCR reaction system is subjected to microdroplet segmentation; reading a fluorescence signal of a PCR amplification product, calculating the initial concentration of the target DNA according to the positive microdroplet proportion, and converting to obtain the concentration of the gametes of the noctiluca in the water sample to be detected. The method can solve the problems of low detection sensitivity, complicated operation and inaccurate quantification of low-abundance samples in the prior art.

Breeding method of milch goats and fresh goat milk

Resumen de: CN122423508A

A milch goat feeding method relates to the technical field of feeding, the milch goat feeding method comprises the following steps: feeding fodder including coarse fodder, concentrated fodder and a selenium preparation, adding the coarse fodder and the concentrated fodder according to four seasons in the growth period and the recovery period of milch goats for conventional feeding, and reducing the coarse fodder, increasing the concentrated fodder and adding the selenium preparation in the pregnancy period and the milk production period; the concentrated feed comprises corn, soybean meal, bran and trace elements, the trace elements comprise multivitamins, salts and a calcium preparation, the selenium preparation is a fresh microalgae wet material rich in organic selenium, and the calcium preparation comprises one or more of calcium hydrophosphate, bone meal and soybean curb residues. The milch goats are fed with compound feed composed of coarse feed, concentrated feed and selenium-rich fresh microalgae, and fresh goat milk rich in organic selenium and calcium is obtained.

Method and device for enhancing segmented carbon sequestration performance of microalgae

Resumen de: CN122427793A

The invention provides a method and device for enhancing the segmented carbon sequestration performance of microalgae, and the method comprises the following steps: mixing and dispersing CO2-containing gas and a culture solution in a Venturi tube by using a composite circulating gas supply structure formed by the Venturi tube and a photobioreactor, and then refluxing into the photobioreactor; the method comprises the following steps: regulating and controlling plant hormones by stages according to physiological statuses and culture targets in different growth stages in a microalgae culture process: not adding the plant hormones in an early stage or adding the plant hormones with the addition amount lower than that in a middle stage; in the middle stage, plant hormones are added to enhance photosynthesis, cell division and carbon assimilation ability of microalgae; in the later stage, according to the continuous carbon sequestration or target product accumulation requirement, the plant hormone adding amount and adding frequency are adjusted or adding is stopped; the culture temperature, the illumination intensity, the light-dark period, the CO2 concentration and the circulation flow are controlled. According to the method, the microalgae biomass accumulation, the chlorophyll content, the CO2 utilization rate and the light energy utilization efficiency can be remarkably improved.

Ecological treatment method for shrimp culture tail water

Resumen de: CN122403651A

The invention belongs to the technical field of wastewater treatment, and discloses an ecological treatment method for shrimp culture tail water, which comprises the following steps: S1, introducing the shrimp culture tail water subjected to precipitation and filtration treatment into a primary ecological pool, inoculating macroalgae into the primary ecological pool, and carrying out primary purification treatment on the shrimp culture tail water; s2, introducing the preliminarily purified prawn culture tail water into a secondary ecological pond, inoculating microalgae into the secondary ecological pond, and performing secondary purification treatment on the prawn culture tail water; the microalgae comprise dunaliella salina and chlorella, the initial inoculum size of the dunaliella salina and the chlorella is 1: (2-4), and the molar ratio of nitrogen to phosphorus in the water body in the secondary ecological pond is (12-14): 1; s3, introducing the shrimp culture tail water subjected to secondary purification into a third-stage ecological pond, and putting oysters and the shrimp culture tail water into the third-stage ecological pond for third-stage purification treatment. The total nitrogen, the total phosphorus, the chemical oxygen demand and the suspended matter content in the shrimp culture tail water treated by the treatment method are greatly reduced, and the effluent quality reaches the discharge standard.

Method for improving microalgae biomass and eicosapentaenoic acid yield through amino acid regulation

Resumen de: CN122405434A

The invention belongs to the fields of microalgae biotechnology, microalgae culture regulation and control and high-value lipid biomanufacturing, and particularly relates to a method for improving microalgae biomass and eicosapentaenoic acid yield through amino acid regulation and control, which comprises the following steps: by taking microalgae capable of synthesizing EPA as a culture object, carrying out amino acid regulation and control under a non-stress culture condition containing sufficient inorganic nitrogen sources, and carrying out amino acid regulation and control on the culture object to obtain the eicosapentaenoic acid. Exogenous amino acids or amino acid combinations are added into a culture system, photosynthesis, growth metabolism and lipid synthesis processes of the microalgae are regulated and controlled, and the microalgae biomass and EPA volume yield are synchronously increased; according to the invention, the specific exogenous amino acid or amino acid combination is added under the culture condition of sufficient nutrition, so that the synchronous promotion of microalgae growth and EPA accumulation is realized. Different from a traditional method for inducing lipid accumulation depending on nitrogen deficiency and other stress, the method can improve the microalgae biomass, photosynthetic performance, pigment yield and EPA volume yield under the condition that cell growth and photosynthetic activity are not obviously inhibited.

Microalgae sterilization sealing tank

Resumen de: CN224513494U

The utility model belongs to the technical field of microalgae sterilization, and particularly relates to a microalgae sterilization sealing tank which comprises a tank body, a sealing cover is mounted at the top of the tank body, a protective cover is sleeved on the surface of the tank body, a pipe valve is arranged at the bottom of the protective cover, penetrates through the protective cover and is communicated with the bottom of the tank body, and an ultraviolet sterilization lamp is arranged in the protective cover. A temperature sensor is arranged at the top of the sealing cover, the bottom of the temperature sensor penetrates into the tank body, a control module is arranged on one side of the protective cover and electrically connected with the ultraviolet germicidal lamp and the temperature sensor, one-way valves are arranged at the top of the sealing cover and communicate with the tank body, and the number of the one-way valves is two. The protective cover is divided into a culture solution inlet and a gas exchange port, and the inner wall of the protective cover is coated with a reflective coating. The utility model provides a microalgae sterilization sealing tank which is a sealed microalgae culture tank which is compact in structure and can realize sterilization without opening a cover.

Mine repairing method

Resumen de: CN122397412A

The invention belongs to the technical field of mine reclamation and ecological restoration, and particularly relates to a mine restoration method. The invention provides a mine remediation method which comprises the step of applying active algae liquid of nitrogen-fixing cyanobacteria with specific concentration and matured compost to a to-be-remedied mine, and the mine remediation method combines organic improvement of a bottom layer with biological crust of a surface layer. By applying the matured compost, the physicochemical properties of the mine matrix can be quickly improved, the porosity, the water holding capacity and basic nutrients are increased, and a favorable microenvironment is created for colonization of blue-green algae on the surface layer. Meanwhile, the formation of the blue-green algae crust can fix nitrogen in the atmosphere and secrete exopolysaccharides, so that the surface soil is further improved, water and soil loss is prevented, and the positive succession of an ecological system is further accelerated. And the mine remediation method is green, ecological and free of pollution, and meets the requirement of sustainable development.

Wall breaking equipment for extracting microalgae protein from seaweed

Resumen de: CN122405423A

The invention relates to the technical field of protease extraction, in particular to wall breaking equipment for extracting microalgae protein from seaweed, the wall breaking equipment comprises a filter box and a liquid inlet pipe, and the output end of the liquid inlet pipe extends into the filter box; the filtering device further comprises a filtering bag, a roller, a roller frame, a sliding frame, a first driving mechanism and a second driving mechanism, the sliding frame is vertically installed on the inner wall of the filtering box, the first driving mechanism and the second driving mechanism are both installed on the sliding frame, the filtering bag is made of an ultrafiltration membrane, and a bag opening of the filtering bag is connected with the first driving mechanism; the first driving mechanism drives the filter bag to enter and exit from the filter chamber of the filter box and conveys a bag opening of the filter bag to the position below the output end of the liquid inlet pipe, the two rollers are installed on the roller frame and make rolling contact with the outer walls of the two sides of the filter bag respectively, the roller frame is connected with the second driving mechanism, and the second driving mechanism drives the two rollers to move up and down.

Novel Microalgae and Microalgal Compositions

Resumen de: US20260201427A1

0000 Provided herein are cultures comprising eukaryotic microorganisms having a lipid profile comprising eicosapentaenoic acid. Also provided are compositions comprising the eukaryotic microorganisms as well as methods of using the eukaryotic microorganisms.

USE OF MICROALGAE AND MICROALGAE DERIVED COMPONENTS IN CULTIVATED FOOD PRODUCTION

Resumen de: US20260201313A1

Provided herein are methods for cultivated food production involving the use of microalgae. The methods include recycling spent cell media that are used to cultivate cells for cultivated food production as well as enriching culture medium used to cultivate the same cells. The microalgae are used to recycle the spent culture medium. The microalgae may be used to extract microalgae derived components to enrich the culture medium. The algae recycling and algae enriched growth media as provided herein may be used with any animal cell culture. In some variations, the microalgae or microalgae derived components may be used as a raw ingredient in the preparation of cell based seafood products. The methods provided herein may be used for the production of seafood products. The methods herein may provide at least one added advantage—the use of algae may add nutrients, flavors, and aromas typically desired in seafood.

Method for quickly starting phycomycete biofilm for treating mariculture tail water based on plant hormone regulation and control

Resumen de: CN122382052A

The invention discloses a phycomycete biofilm rapid starting method for treating mariculture tail water based on plant hormone regulation and control. The method comprises the following steps: 1, dilution and domestication pretreatment of activated sludge; 2, carrier pretreatment and bacterial biofilm pre-attachment; 3, culturing and enriching the seawater chlorella; 4, preparing a plant hormone working solution; and 5, quickly starting culture of the phycomycetes biological membrane. Through a staged strategy of activated sludge pre-biofilm formation and regulation and control of microalgae colonization by plant hormones, the starting time of mariculture tail algae bacteria biofilm is shortened to about 20 days from traditional 30-56 days, and the starting efficiency is improved by more than 50%; the used raw materials are common reagents, the cost is low, the operation steps are simple, and large-scale popularization and application are easy; a biological enhancement means is adopted, use of chemical disinfectants or harmful fungicide is not involved, the plant hormones can be naturally degraded after playing a promoting role, and secondary pollution to the mariculture environment is avoided.

Cultivation method of initial feed for Dongtaotai

Resumen de: CN122375519A

The invention provides a cultivation method of initial feed for Dongquasicera japonica. The cultivation method comprises the steps of composite microalgae collaborative cultivation, rotifer sterile domestication, gradient nutrient enrichment, probiotic field planting and low-temperature stabilization treatment. The method comprises the following steps: carrying out composite culture on chlorella, isochrysis sp. 3011 and chaetoceros mulleri, combining penicillin-streptomycin aseptic treatment and third-generation domestication, carrying out gradient reinforcement by adopting a composite microalgae liquid, a first reinforcer and a second reinforcer, adding composite probiotics for colonization, and carrying out low-temperature stabilization treatment by sodium alginate and sodium ascorbate. The DHA content and the astaxanthin content of the bait are remarkably increased, the fatty acid oxidation rate is reduced, the probiotic colonization amount is increased, the survival rate of the east astrostroma fry reaches 78.3%, the average body length is 5.2 mm, the intestinal villus height is 181 mu m, the enteritis morbidity is 2.7%, the anti-stress survival rate is 93.2%, and a high-quality bait solution is provided for large-scale fry breeding.

Illumination culture method for improving biomass of chlorella pyrenoidosa

Resumen de: CN122381929A

The invention relates to an illumination culture method for improving the biomass of chlorella pyrenoidosa, which comprises the following steps: inoculating a chlorella pyrenoidosa stock solution into a sterile liquid culture medium, and carrying out illumination culture on the chlorella pyrenoidosa by using a white light and red light composite spectrum. The invention develops the culture method for the chlorella pyrenoidosa, namely, the chlorella pyrenoidosa is subjected to illumination culture by using a composite spectrum of white light and red light, the culture method is simple to operate and easy to implement, and the method can obviously improve the growth rate and biomass accumulation of the chlorella pyrenoidosa, so that the yield of the chlorella pyrenoidosa is increased. The high-quality large-scale production of the chlorella pyrenoidosa is And the production energy consumption of unit biomass can be reduced, and the technical problem that yield increase and consumption reduction are difficult to consider in microalgae culture is solved.

Underwater illuminating lamp assembly for microalgae culture and photobioreactor

Nº publicación: CN122384042A 14/07/2026

Solicitante:

QINGDAO INST OF BIOENERGY AND BIOPROCESS TECHNOLOGY CHINESE ACADEMY OF SCIENCES
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Resumen de: CN122384042A

The invention relates to an underwater illuminating lamp assembly for microalgae culture, and also relates to a photobioreactor comprising the underwater illuminating lamp assembly. According to the underwater illuminating lamp assembly, 360-degree light source layout can be achieved, a stirring device and a ventilation device can be integrated, and the functions of illumination, stirring, aeration and the like of the photobioreactor are achieved. The underwater illuminating lamp assembly can take away heat generated by the lamp in time, reduce the temperature of the lamp tube, reduce the influence of high temperature on light emission and the service life of the lamp, reduce heat transfer of the heat of the lamp tube to a microalgae culture solution and reduce temperature rise of a microalgae culture system. The hyperboloid stirring paddle and the paddle surface gas distribution holes are adopted, so that the mixing is uniform, the gas-liquid contact is sufficient, the damage of stirring and shearing to algae cells is reduced, and the stirring energy consumption is low. In addition, the underwater lamp assembly is simple in structure, can be directly applied to a large microalgae culture container to form a photobioreactor, and is easy to remove, expand in scale and replace parts.